Plant composition with anti-inflammatory and soothing effects as well as preparation method and application thereof

Abstract

The invention discloses a plant composition with anti-inflammatory and soothing effects as well as a preparation method and application thereof, the plant composition comprises a Chinese herbaceous peony root extract, a polygonatum cyrtonema rhizome / root extract and a gentiana macrophylla extract, and the components are uniformly mixed during preparation; the Chinese herbaceous peony root extract, the polygonatum cyrtonema rhizome / root extract and the gentiana macrophylla extract are compounded, an unexpected excellent effect is obtained, particularly, the antioxidant relieving and lasting effects are obviously improved, meanwhile, the irritation is low, and the composition is suitable for wider skin types and can effectively cope with various inflammation inducements; after the three components are compounded, a channel from the early stage to the late stage of inflammatory response is covered, the limitation of a single target spot is broken through, and the composition has the effects of resisting aging and repairing after-sun injury and can be used for preparing various cosmetics.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products, specifically to a plant composition with anti-inflammatory and soothing effects, its preparation method, and its application. Background Technology

[0002] In recent years, plant extracts have been widely used in the cosmetics industry due to their natural origin, low irritation, and multi-functionality. Many plants contain anti-inflammatory, soothing, and repairing properties. However, current anti-inflammatory and soothing cosmetics often rely on single plant extracts (such as purslane and centella asiatica) or synthetic hormones, which have issues such as limited target action and insufficient safety with long-term use. Meanwhile, some products rely on synthetic hormones, which can effectively relieve inflammation in the short term, but long-term use may lead to skin side effects and safety concerns. Furthermore, both immediate and long-lasting anti-inflammatory and soothing effects are not adequately addressed, especially regarding the limited types of inflammation they are suitable for, making it difficult to meet the diverse needs of different consumers. Summary of the Invention

[0003] The purpose of this invention is to overcome one or more shortcomings in the prior art and provide a new plant composition with anti-inflammatory and soothing effects.

[0004] The present invention also provides a method for preparing the above-mentioned plant composition with anti-inflammatory and soothing effects and its application in the preparation of cosmetics with anti-inflammatory and soothing effects.

[0005] To achieve the above objectives, the present invention employs the following technical solution:

[0006] A plant composition with anti-inflammatory and soothing effects, the plant composition comprising peony root extract, Solomon's seal rhizome / root extract and Gentiana macrophylla extract.

[0007] In some embodiments of the present invention, the mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5-9:2-5:1.

[0008] Furthermore, the mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5-7:3-4.5:1.

[0009] Furthermore, the mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5.5-6.5:3.5-4.5:1.

[0010] According to certain aspects of the present invention, the mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5-9∶2-5∶1.

[0011] The feeding ratio range is 5-9, including but not limited to 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, etc.;

[0012] The feeding ratio ranges from 2 to 5, including but not limited to 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, etc.

[0013] In this invention, peony root extract is an extract obtained from peony roots. Furthermore, the peony root extract can be commercially available or prepared according to the following method;

[0014] The peony root extract is prepared by the following method: peony root powder is extracted using an aqueous alcohol solvent as the solvent, the extract is collected and purified, and then dried.

[0015] In some embodiments of the present invention, during the extraction process of the peony root extract, the alcohol solvent in the aqueous alcohol solvent solution may include, but is not limited to, ethanol; the volume percentage of alcohol in the aqueous alcohol solvent solution may be 60 vol%-80 vol%.

[0016] In some embodiments of the present invention, during the extraction process of the peony root extract, the mass ratio of peony root powder to the volume ratio of the alcohol solvent aqueous solution is 1 (g): 6-9 (mL).

[0017] In some embodiments of the present invention, during the extraction of the peony root extract, the solvent extraction process is carried out in an ultrasonic treatment; further, the ultrasonic treatment is controlled at a power of 150-400W and the treatment time is 30-40 minutes.

[0018] In some embodiments of the present invention, during the extraction of the peony root extract, the extract is obtained by filtering a mixture after ultrasonic treatment and then concentrating it until the solid content in the concentrate is 50 wt.% or more (e.g., 50 wt.%-60 wt.%). Further, the impurity removal process involves passing the concentrate through a macroporous adsorption resin, first eluting it with deionized water until the eluent has no obvious color, then eluting it with an aqueous ethanol solution (volume concentration can be 30 vol%-50 vol%), and collecting the eluent. Even further, the collected eluent is concentrated under reduced pressure at 60-70°C until the solid content is 50 wt.% or more (e.g., 50 wt.%-60 wt.%), thus obtaining a concentrated extract again.

[0019] In some embodiments of the present invention, during the extraction process of the peony root extract, the concentrated solution obtained by concentrating the eluent is subjected to vacuum freeze-drying to obtain the peony root extract.

[0020] In this invention, the rhizome / root extract of Polygonatum multiflorum refers to the extract from the rhizome / root of each Polygonatum multiflorum plant. Further, the rhizome / root extract of Polygonatum multiflorum can be commercially available or prepared according to the following method;

[0021] The rhizome / root extract of Polygonatum multiflorum was prepared by the following method:

[0022] The washed rhizomes / roots of Polygonatum odoratum are steamed and dried in water, repeated several times until they turn black, and then Polygonatum odoratum powder is made.

[0023] Water was used as a solvent to extract Polygonatum odoratum powder by heating. The extract was concentrated and then an alcohol solvent or its aqueous solution was added to precipitate the crude product.

[0024] The crude product was dispersed in a mixture of water, chlorinated alkanes and alcohol, allowed to stand and separate into layers, and the supernatant was collected.

[0025] Add an alcohol solvent to the supernatant, precipitate, and dry the precipitate.

[0026] In some embodiments of the present invention, during the extraction process of the Polygonatum odoratum rhizome / root extract, the temperature of the water-bath steaming is 95-100°C, and the product can be dried in the sun until completely dry. Furthermore, the extraction can be repeated 8-10 times.

[0027] In some embodiments of the present invention, during the extraction process of the Polygonatum rhizome / root extract, when water is used as the solvent for heating extraction, the mass ratio of Polygonatum powder to water is 1 (g): 6-9 (mL). Further, the heating extraction temperature is 70-80°C, and even further, the heating extraction time can be 40-60 min, etc.

[0028] In some embodiments of the present invention, during the extraction process of the rhizome / root extract of Polygonatum odoratum, the extract is filtered to obtain a filtrate, which is then concentrated to a solid content of 50 wt.% or more (e.g., 50 wt.%-60 wt.%) to obtain a concentrate; further, the concentration process is carried out by vacuum concentration at 70-80°C in a rotary evaporator to a solid content of 50 wt.% or more (e.g., 50 wt.%-60 wt.%).

[0029] In some embodiments of the present invention, during the extraction process of the Polygonatum odoratum rhizome / root extract, the alcohol solvent or its aqueous solution added to the concentrate can be ethanol or an aqueous solution of ethanol; further, when adding the aqueous solution of alcohol solvent, the volume percentage of alcohol solvent in the final mixed system reaches 75 vol%-85 vol%.

[0030] In some embodiments of the present invention, during the extraction process of the rhizome / root extract of Polygonatum odoratum, an alcohol solvent or its aqueous solution is added to the concentrate, stirred evenly, allowed to stand at 1-10°C (moreover 2-6°C), and then filtered to obtain a precipitate, which is the crude product (Polygonatum odoratum crude polysaccharide).

[0031] In some embodiments of the present invention, during the extraction process of the rhizome / root extract of Polygonatum odoratum, the crude product is first dispersed in water, and then a mixed solvent of chloroalkane and alcohol is added; further, the crude product and water are mixed at a solid-liquid ratio of 1:15-25 (more specifically, 1:18-22); even further, the chloroalkane and alcohol are compounded at a volume ratio of 2-4:1 to prepare a mixed solvent. According to some specific aspects of the present invention, the chloroalkane can be chloroform, etc., and the alcohol can be n-butanol, etc.

[0032] In some embodiments of the present invention, during the extraction process of the Polygonatum odoratum rhizome / root extract, the static stratification can be performed multiple times, and the supernatant is finally combined.

[0033] In some embodiments of the present invention, during the extraction process of the rhizome / root extract of Polygonatum odoratum, an alcohol solvent (including but not limited to anhydrous ethanol) is slowly added dropwise to the supernatant so that the volume ratio of the alcohol solvent in the mixed system reaches 75 vol%-85 vol%.

[0034] In some embodiments of the present invention, during the extraction process of the Polygonatum odoratum rhizome / root extract, an alcohol solvent is added to the supernatant, and the mixture is allowed to stand at 1-10°C (moreover, 2-6°C), then filtered and freeze-dried under vacuum to obtain the Polygonatum odoratum rhizome / root extract.

[0035] In this invention, Gentiana macrophylla extract refers to an extract obtained from the whole Gentiana macrophylla plant. Further, the Gentiana macrophylla extract can be commercially available or prepared according to the following method;

[0036] The Gentiana macrophylla extract was prepared by the following method: Gentiana macrophylla powder was extracted by heating with water as solvent, the extract was collected and purified, and then dried.

[0037] In some embodiments of the present invention, during the extraction process of the Gentiana macrophylla extract, dried Gentiana macrophylla is weighed, washed, dried at 60°C to constant weight, and pulverized to obtain Gentiana macrophylla powder.

[0038] In some embodiments of the present invention, during the extraction process of the Gentiana macrophylla extract, when water is used as the solvent for heating extraction, the mass ratio of Gentiana macrophylla powder to water is 1 (g): 6-9 (mL). Further, the heating extraction operation can involve heating the liquid to boiling, reflux extraction, filtering, and collecting the filtrate.

[0039] In some embodiments of the present invention, during the extraction process of the Gentiana macrophylla extract, the collected filtrate is concentrated to a solid content of 50 wt.% or more (e.g., 50 wt.%-60 wt.%) to obtain a concentrate; further, the concentration process is to concentrate under reduced pressure at 70-80°C in a rotary evaporator to a solid content of 50 wt.% or more (e.g., 50 wt.%-60 wt.%).

[0040] In some embodiments of the present invention, during the extraction of the Gentiana macrophylla extract, the impurity removal process involves passing the concentrated solution through a macroporous adsorption resin, first eluting with deionized water until the eluent has no obvious color, then eluting with an aqueous ethanol solution (volume concentration can be 30 vol%-50 vol%), and collecting the eluent. Further, the collected eluent is concentrated under reduced pressure at 60-70°C to a solid content of 50 wt.% or more (e.g., 50 wt.%-60 wt.%), to obtain a concentrated solution again.

[0041] In some embodiments of the present invention, during the extraction process of the Gentiana macrophylla extract, the concentrated solution obtained by concentrating the eluent is subjected to vacuum freeze-drying to obtain Paeonia lactiflora root extract.

[0042] Another technical solution provided by the present invention is a method for preparing the above-mentioned plant composition with anti-inflammatory and soothing effects, wherein the preparation method includes: mixing and blending the components to obtain the plant composition.

[0043] Another technical solution provided by the present invention: the application of the above-mentioned plant composition with anti-inflammatory and soothing effects in the preparation of cosmetics with anti-inflammatory and soothing effects.

[0044] In some embodiments of the present invention, the plant composition comprises 0.1%-5% by weight in the cosmetic, more specifically 0.5%-4%.

[0045] According to certain aspects of the present invention, the plant composition in the cosmetic product comprises, by weight percentage, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, 2.1%, ... 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5%.

[0046] In some embodiments of the present invention, the cosmetic is a face cream, moisturizing cream, face mask, serum, etc.

[0047] According to some specific aspects of the present invention, when the cosmetic is a face cream, it comprises water, alcohol, surfactant, thickener, the above-mentioned plant composition having anti-inflammatory and soothing effects, oil and / or wax, etc.

[0048] In some embodiments of the present invention, the face cream contains, by weight percentage, 0.5%-10% alcohol, 0.01%-0.3% surfactant, 0.1%-2% thickener, 0.1%-5% of the above-mentioned plant composition with anti-inflammatory and soothing effects, 10%-40% oil and / or wax, and water to make up to 100%.

[0049] Furthermore, in face creams, alcohols may include panthenol, 1,2-hexanediol, etc.

[0050] Furthermore, in the face cream, the surfactant can be anionic surfactant, cationic surfactant, amphoteric surfactant, etc.; more preferably, in the face cream, the surfactant can be sodium stearoyl glutamate.

[0051] Furthermore, in the face cream, the thickener can be a natural thickener, a synthetic or semi-synthetic thickener; more preferably, in the face cream, the thickener can be an ammonium acryloyldimethyl taurate / VP copolymer.

[0052] Furthermore, in the face cream, the oil and / or wax may include, but are not limited to, polyglycerol-3-methylglucose distearate, rice bran oil, and phytosterol isostearyl dioleate. Even further, according to some specific aspects of the present invention, the oil and / or wax of the present invention are composed of polyglycerol-3-methylglucose distearate, rice bran oil, and phytosterol isostearyl dioleate, wherein the mass ratio of the polyglycerol-3-methylglucose distearate, the rice bran oil, and the phytosterol isostearyl dioleate is 1:3-8:0.05-0.5.

[0053] Furthermore, the cream also contains preservatives, including but not limited to p-hydroxyacetophenone. Even further, the preservatives comprise 0.1%-1% of the cream by weight.

[0054] Furthermore, by weight percentage, the face cream contains 1%-5% panthenol, 0.2%-1% 1,2-hexanediol, 0.1%-1% p-hydroxyacetophenone, 0.01%-0.3% sodium stearoyl glutamate, 0.1%-2% ammonium acryloyl dimethyl taurate / VP copolymer, 0.1%-5% of the above-mentioned plant composition with anti-inflammatory and soothing effects, 1%-5% polyglycerol-3-methylglucose distearate, 10%-33% rice bran oil, 0.1%-2% phytosterol isostearyl alcohol dimeric linoleate, and water to make up to 100%.

[0055] Furthermore, face creams may also contain moisturizers, skin conditioning agents, etc. Moisturizers may be polyol moisturizers or hyaluronic acid, etc., and skin conditioning agents may be some synthetic or naturally derived compounds, which will not be elaborated on again.

[0056] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art:

[0057] Given the unstable efficacy of existing anti-inflammatory and soothing cosmetics, their inability to effectively address different types of inflammatory responses, and the lack of a balance between immediate and long-lasting anti-inflammatory and soothing effects, the inventors of this invention, during extensive experimental research, unexpectedly discovered that the combination of peony root extract, Polygonatum rhizome / root extract, and Gentiana macrophylla extract yielded unexpectedly excellent results, particularly in terms of significant enhancement in antioxidant and soothing effects and their long-lasting effects. Furthermore, it exhibits low irritation, is suitable for a wider range of skin types, and effectively addresses various inflammatory triggers. Further research and analysis suggest that the combination of these three extracts covers the early to late pathways of the inflammatory response. The three extracts form a complete pathway coverage in the early (immune activation), middle (mediator release), and late (tissue damage) stages of the inflammatory response, overcoming the limitations of single-target treatments and possessing anti-aging and sun damage repair effects. Attached Figure Description

[0058] Figure 1 This is a comparison chart of the anti-inflammatory test results of peony root extract in the embodiments of the present invention;

[0059] Figure 2 This is a comparison chart of the anti-inflammatory test results of the rhizome / root extract of Polygonatum multiflorum in the embodiments of the present invention;

[0060] Figure 3 This is a comparative diagram showing the effect of Polygonatum multiflorum rhizome / root extract on fibroblasts in an embodiment of the present invention;

[0061] Figure 4 This is a comparison chart of the anti-inflammatory test results of Gentiana macrophylla extract in the embodiments of the present invention;

[0062] Figure 5 This is a comparison chart of the anti-inflammatory test results of various embodiments and comparative examples in this invention;

[0063] Figure 6 This is a test image showing the improved facial soothing effect of the face cream prepared in Application Example 1 of the present invention. Detailed Implementation

[0064] This invention provides an anti-inflammatory and soothing plant composition with a multi-target synergistic mechanism, comprising peony root extract, Polygonatum odoratum rhizome / root extract, and Gentiana macrophylla extract.

[0065] Currently, peony root extract, Polygonatum odoratum rhizome / root extract, and Gentiana macrophylla extract, when used alone, each have different or similar effects.

[0066] For example, peony root extract has repair and anti-inflammatory effects. It contains paeoniflorin and other substances that can inhibit IL-1α and IL-6, and inhibit the H1 receptor effect in anti-allergy.

[0067] For example, the rhizome / root extract of Polygonatum multiflorum has anti-inflammatory and soothing effects, and it can inhibit pro-inflammatory factors such as IL-6 and TNF-α and repair the skin barrier.

[0068] For example, Gentiana macrophylla extract has anti-inflammatory and soothing effects. It contains iridoids such as gentiopicrin, which can inhibit inflammatory factors such as IL-6.

[0069] During extensive experimental research, the inventors of this invention unexpectedly discovered that combining the three elements yielded surprisingly superior results, even better than using any single element alone.

[0070] Furthermore, experimental results showed that the combination of peony root extract, Polygonatum rhizome / root extract, and Gentiana macrophylla extract could achieve the best anti-inflammatory and soothing effects, with the preferred ratio being 5-9:2-5:1, and the combination of the three had a synergistic effect.

[0071] Further, the mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5-7:3-4.5:1. Even further, the mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5.5-6.5:3.5-4.5:1.

[0072] Peony root extract, Polygonatum rhizome / root extract, and Gentiana macrophylla extract are all commercially available or can be prepared using self-made methods.

[0073] The above-mentioned solution will be further described below with reference to specific embodiments; it should be understood that these embodiments are used to illustrate the basic principles, main features and advantages of the present invention, and the present invention is not limited to the scope of the following embodiments; the implementation conditions used in the embodiments can be further adjusted according to specific requirements, and the implementation conditions not specified are usually the conditions in conventional experiments.

[0074] Unless otherwise specified in the following examples, all raw materials are commercially available or prepared by conventional methods in the art.

[0075] The peony root extract described below was prepared according to the following method:

[0076] 1) Take fresh peony root, wash it, slice it, dry it with hot air at 60℃ until constant weight, and then grind it for later use;

[0077] 2) Weigh 10g of peony powder and add 70 vol% ethanol aqueous solution at a material-to-liquid ratio of 1:9 (g / mL). Place in an ultrasonic instrument and perform ultrasonic extraction at 400W for 30 min.

[0078] 3) Filter, collect the extract, and concentrate it under reduced pressure at 65±5℃ on a rotary evaporator until the solid content is about 55wt.%;

[0079] 4) Pass the concentrated solution through a macroporous adsorption resin. First, elute with deionized water until the effluent has no obvious color, then elute with 40 vol% ethanol aqueous solution until no obvious color is found. Collect the eluent.

[0080] 5) The eluent was concentrated under reduced pressure at 65±5℃ until the solid content was about 55wt.%, to obtain the concentrate;

[0081] 6) The concentrate was freeze-dried under vacuum for 48 hours to obtain peony root extract.

[0082] Anti-inflammatory test of peony root extract (sometimes referred to as peony in the following tests):

[0083] The experiment used an ELISA kit to evaluate the anti-inflammatory effect of the samples on mouse monocytes / macrophages (RAW264.7) by detecting the concentrations of IL1-α and IL-6. The detection method is as follows:

[0084] (1) Cell inoculation

[0085] After passaged RAW264.7 cells to the third generation, once the cells had confined the culture flask to a single layer (70%–80%), they were then injected with 1×10⁻⁶ cells per cell line. 5 Inoculate 1 mL per well into 24-well plates at a concentration of 1 mL / mL and incubate in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0086] (2) Cell treatment methods

[0087] Remove the culture medium and add 500 μL of sample solution diluted with culture medium. Divide the samples into four groups: blank control group (cell culture medium treatment), cell inflammation model group (10 μg / mL LPS treatment), positive control group (10 μg / mL LPS + 200 μg / L dexamethasone treatment), and sample group (0.5% peony, 1% peony, 2% peony + 10 μg / mL LPS treatment groups). Continue incubation for 24 h. Centrifuge the culture supernatant for 3 min (4℃, 15000 rpm / min). Detect the IL1-α and IL-6 levels in the supernatant according to the ELISA kit instructions.

[0088] (3) Data Analysis

[0089] All experiments were repeated three times. Results are expressed as mean ± standard deviation (SD). Data were analyzed statistically using one-way ANOVA. A p-value < 0.05 was considered statistically significant.

[0090] The results are shown in Tables 1 and 2, and Figure 1 .

[0091] Table 1. Inhibitory effect of peony on IL1-α

[0092]

[0093] Note: *P<0.05; ** P<0.01; *** P<0.001.

[0094] Table 2. Inhibitory effect of peony on IL-6

[0095]

[0096] Note: *P<0.05; ** P<0.01;*** P<0.001.

[0097] The experimental results showed that, compared with the blank control group, LPS treatment significantly upregulated the expression of inflammatory cytokines IL-1α and IL-6 in mouse monocytes and macrophages, and different concentrations of peony root extract showed good inhibitory effects on both of these inflammatory cytokines. The inhibition rate of IL-1α by 0.5% peony root extract was 25.01%, 1% peony root extract was 22.01%, and 2% peony root extract was 38.43%; the inhibition rate of IL-6 by 0.5% peony root extract was 19.76%, 1% peony root extract was 31.14%, and 2% peony root extract was 18.59%. These results indicate that, in the LPS-induced mouse monocyte and macrophage inflammation model, peony root extract can effectively inhibit the expression of IL-1α and IL-6, thereby exerting an anti-inflammatory effect.

[0098] H1 receptor experiment:

[0099] Histamine H1 receptors are a subtype of histamine receptors, primarily distributed in smooth muscle cells, vascular endothelial cells, the heart, and the central nervous system. The H1 receptor activity inhibition rate reflects the degree to which an active ingredient blocks H1 receptor signal transduction. A higher inhibition rate indicates that the substance is more effective in blocking the inflammatory response induced by histamine-H1 receptor binding.

[0100] The detection method used in this experiment is as follows:

[0101] (1) Cell inoculation

[0102] The cell line used in this experiment was HEK cells. After passaged to the third generation, and once the cells had filled a single layer (70%–80%) in the culture flask, they were cultured at a rate of 1×10⁻⁶ cells / mL. 5 Inoculate 1 mL per well into 24-well plates at a concentration of 1 mL / mL and incubate in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0103] (2) Cell treatment methods

[0104] Remove the culture medium and add 500 μL of sample solution diluted with culture medium. Divide the samples into a blank control group and a sample group (0.05% peony root and 0.09% peony root treatment group), and continue incubation for 24 h. Detect the inhibition rate of H1 receptor activity of the samples according to the ELISA kit instructions.

[0105] (3) Data Analysis

[0106] All experiments were repeated three times. Results are expressed as mean ± standard deviation (SD). Data were analyzed statistically using one-way ANOVA. A p-value < 0.05 was considered statistically significant.

[0107] The results are shown in Table 3.

[0108] Table 3. Inhibition of H1 receptors by different concentrations of peony.

[0109] Sample Name Inhibition rate (%) ± SD p-value Blank control group -0.13±0.59 / 0.05% peony 0.13±0.65 0.638 0.09% peony 6.11±2.04 0.007**

[0110] Note: Compared with the blank control group, **P<0.01.

[0111] The results of the inhibition rate of peony on H1 receptor activity showed that 0.09% of peony had a good inhibitory effect on H1 receptors, with an inhibition rate of 6.11%. This indicates that peony can effectively block the binding of histamine-H1 receptors, thereby inhibiting the inflammatory response.

[0112] The rhizome / root extract of Polygonatum multiflorum was prepared according to the following method:

[0113] 1) Take fresh rhizomes / roots of Polygonatum multiflorum and wash them clean;

[0114] 2) After washing, the Polygonatum multiflorum is steamed and dried nine times: steamed for 2 hours (100℃) in water, then dried in the sun until completely dry. Repeat this process 9 times until the color turns black. Grind it into Polygonatum powder for later use.

[0115] 3) Weigh 10g of Polygonatum powder, add deionized water at a material-to-liquid ratio of 1:8 (g / mL), heat and extract for 50 minutes, with the temperature controlled within the range of 75±5℃;

[0116] 4) After filtration, the extracts and filtrates were combined and concentrated under reduced pressure at 75±5℃ in a rotary evaporator until the solid content was about 55wt.%.

[0117] 5) Slowly add the concentrated solution dropwise to a 95% ethanol aqueous solution to make the final ethanol volume fraction reach 80 vol%. Stir well, let stand at 4°C for 8 hours, filter, collect the precipitate, and obtain the crude product.

[0118] 6) Take the crude product, dissolve it in an appropriate amount of distilled water (solid-liquid ratio 1:20, g / mL) to obtain a mixture, then add the same volume of chloroform-n-butanol (3:1, v / v) to the mixture, shake for 5 min, let stand for separation, take the supernatant, repeat 3 times, and combine the supernatants.

[0119] 7) Slowly add anhydrous ethanol dropwise to the supernatant to make the final volume fraction of ethanol 80%. Let it stand at 4-5℃ for 8 hours, filter and freeze dry under vacuum to obtain Polygonatum odoratum rhizome / root extract.

[0120] Anti-inflammatory test of Polygonatum odoratum rhizome / root extract (sometimes referred to as Polygonatum odoratum in the following tests):

[0121] The anti-inflammatory effects of the samples on mouse monocytes / macrophages (RAW264.7) were evaluated using an ELISA kit by detecting the concentrations of IL-6 and TNF-α. The detection methods are as follows:

[0122] (1) Cell inoculation

[0123] After passaged RAW264.7 cells to the third generation, once the cells had confined the culture flask to a single layer (70%–80%), they were then injected with 1×10⁻⁶ cells per cell line. 5 Inoculate 1 mL per well into 24-well plates at a concentration of 1 mL / mL and incubate in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0124] (2) Cell treatment methods

[0125] Remove the culture medium and add 500 μL of sample solution diluted with culture medium. Divide the samples into four groups: blank control (cell culture medium treatment), cell inflammation model group (10 μg / mL LPS treatment), positive control group (10 μg / mL LPS + 200 μg / L dexamethasone treatment), and sample group (0.063% Polygonatum sibiricum, 0.125% Polygonatum sibiricum, 0.25% Polygonatum sibiricum + 10 μg / mL LPS treatment groups). Continue incubation for 24 h. Centrifuge the culture supernatant for 3 min (4℃, 15000 rpm / min), and detect the levels of IL-6 and TNF-α in the supernatant according to the ELISA kit instructions.

[0126] (3) Data Analysis

[0127] All experiments were repeated three times. Results are expressed as mean ± standard deviation (SD). Data were analyzed statistically using one-way ANOVA. A p-value < 0.05 was considered statistically significant.

[0128] The results are shown in Tables 4 and 5, and Figure 2 .

[0129] Table 4. Inhibitory effect of different concentrations of Polygonatum on IL-6

[0130]

[0131] Note: *P<0.05; ** P<0.01; ***P<0.001.

[0132] Table 5. Inhibitory effects of different concentrations of Polygonatum sibiricum on TNF-α.

[0133] Note: *P<0.05; ** P<0.01; *** P<0.001.

[0134] The experimental results showed that, compared with the blank control group, LPS treatment significantly upregulated the expression of inflammatory cytokines IL-6 and TNF-α in mouse monocytes and macrophages, and that different concentrations of Polygonatum sibiricum treatment had a good inhibitory effect on both of these inflammatory cytokines. The inhibition rate of IL-6 by 0.063% Polygonatum sibiricum was 55.81%, 56.16% by 0.125% Polygonatum sibiricum, and 57.32% by 0.25% Polygonatum sibiricum; the inhibition rate of TNF-α by 0.063% Polygonatum sibiricum was 34.83%, 36.05% by 0.125% Polygonatum sibiricum, and 44.18% by 0.25% Polygonatum sibiricum. These results indicate that in the LPS-induced mouse monocyte-macrophage inflammation model, Polygonatum sibiricum can effectively inhibit the expression of IL-6 and TNF-α, thereby exerting an anti-inflammatory effect.

[0135] Test on the effects of Polygonatum on fibroblasts:

[0136] Fibroblasts maintain skin barrier homeostasis by synthesizing collagen and various extracellular matrix components, and promote tissue repair and barrier restoration through proliferation under inflammatory conditions, thus playing a positive role in skin homeostasis and inflammation relief. This experiment used the 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) method to detect the effect of samples on fibroblast proliferation. The detection method is as follows:

[0137] (1) Cell inoculation

[0138] The cells used in this experiment were fibroblasts. After passaged to the third generation, and once the cells had covered a single layer (70%–80%) in the culture flask, they were cultured at a rate of 1×10⁻⁶ cells / mL. 5 Inoculate 1 mL per well into 24-well plates at a concentration of 1 mL / mL and incubate in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0139] (2) Cell treatment methods

[0140] Each group of cells was divided into 1×10 5Cells were seeded at a concentration of [number] cells / mL into 24-well plates, and 500 μL of sample solution diluted with culture medium was added to each well. The plates were divided into a blank control group, a UVB irradiation group, and sample groups (0.01% Polygonatum sibiricum, 0.03% Polygonatum sibiricum, and 0.05% Polygonatum sibiricum treatment groups), and incubation was continued for 24 h. Before UVB irradiation, the culture medium was removed, and the cells were washed twice with phosphate-buffered saline (PBS), followed by PBS to cover the cells. UVB irradiation was performed using a Vilber Lourmat (Marne la Vallee, France) device, with the radiation dose set to 400 mJ / cm². 2 After irradiation, the cells were incubated in a CO2 incubator (37°C, 5% CO2) for 24 hours. Then, MTT solution to a final concentration of 0.5 mg / mL was added to the cells, and the cells were incubated at 37°C for 1 hour. The solution was discarded, and the resulting blue-purple formazan crystals were dissolved in dimethyl sulfoxide (DMSO). Finally, the absorbance was measured at 570 nm using an enzyme-linked immunosorbent assay (ELISA) reader (Bio Tek, USA) to calculate cell viability.

[0141] (3) Data Analysis

[0142] All experiments were repeated three times. Results are expressed as mean ± standard deviation (SD). Data were analyzed statistically using one-way ANOVA. A p-value < 0.05 was considered statistically significant.

[0143] See results Figure 3 As shown in the figure. The effect of Polygonatum sibiricum on fibroblast proliferation was assessed using the MTT assay. The results showed that fibroblast survival rate was significantly reduced after UVB irradiation compared to the untreated control group. However, cells treated with 0.01%, 0.03%, and 0.05% Polygonatum sibiricum and then irradiated with the same amount of UVB showed a significant proliferation effect compared to the UVB control group.

[0144] The Gentiana macrophylla extract described below was prepared using the following method:

[0145] 1) Weigh the dried Gentiana macrophylla, wash it, dry it at 60℃ to constant weight, pulverize it to obtain Gentiana macrophylla powder, and set it aside;

[0146] 2) Weigh 10g of Gentiana macrophylla powder and add deionized water at a material-to-liquid ratio of 1:8 (g / mL). Heat to boiling and reflux for 2 hours. Filter and collect the filtrate.

[0147] 3) Concentrate the filtrate under reduced pressure at 75±5℃ using a rotary evaporator until the solid content is approximately 55wt.%;

[0148] 4) Pass the concentrated solution through a macroporous adsorption resin, first elute with deionized water until the eluent has no obvious color, then elute with 40 vol% ethanol aqueous solution until no obvious color is found, and collect the eluent.

[0149] 5) The collected eluent was concentrated under reduced pressure at 65±5℃ until the solid content was about 55wt.%, to obtain a concentrated solution;

[0150] 6) The concentrate was freeze-dried under vacuum for 48 hours to obtain Gentiana macrophylla extract.

[0151] Anti-inflammatory test of Gentiana macrophylla extract (sometimes referred to as Gentiana macrophylla in the following tests):

[0152] The ELISA kit was used to evaluate the anti-inflammatory effect of Gentiana macrophylla on mouse monocytes / macrophages (RAW264.7) by detecting IL-6 concentration. The detection method is as follows:

[0153] (1) Cell inoculation

[0154] After passaged RAW264.7 cells to the third generation, once the cells had confined the culture flask to a single layer (70%–80%), they were then injected with 1×10⁻⁶ cells per cell line. 5 Inoculate 1 mL per well into 24-well plates at a concentration of 1 mL / mL and incubate in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0155] (2) Cell treatment methods

[0156] Remove the culture medium and add 500 μL of sample solution diluted with culture medium. Divide the samples into three groups: a blank control group (cell culture medium treatment), a cell inflammation model group (20 μg / mL LPS treatment), and sample groups (0.5% Gentiana macrophylla, 1% Gentiana macrophylla, and 3% Gentiana macrophylla + 20 μg / mL LPS treatment groups). Continue incubation for 24 h. Centrifuge the culture supernatant for 3 min (4℃, 15000 rpm / min). Detect the IL1-α and IL-6 levels in the supernatant according to the ELISA kit instructions.

[0157] (3) Data Analysis

[0158] All experiments were repeated three times. Results are expressed as mean ± standard deviation (SD). Data were analyzed statistically using one-way ANOVA. A p-value < 0.05 was considered statistically significant.

[0159] The results are shown in Table 6 and... Figure 4 As shown.

[0160] Table 6. Inhibitory effects of different concentrations of Gentiana macrophylla on IL-6.

[0161] Note: *P<0.05;** P<0.01; *** P<0.001.

[0162] The experimental results showed that, compared with the blank control group, LPS treatment significantly upregulated the expression of the inflammatory cytokine IL-6 in mouse monocytes and macrophages, and different concentrations of Gentiana macrophylla treatment all showed good inhibitory effects on IL-6. At the highest concentration of 3%, the inhibition rate reached 91.19%. These results indicate that in the LPS-induced mouse monocyte and macrophage inflammation model, Gentiana macrophylla extract can effectively inhibit the expression of the inflammatory cytokine IL-6, thereby exerting an anti-inflammatory effect.

[0163] Example 1

[0164] This example provides a plant composition with anti-inflammatory and soothing effects and its preparation method. The plant composition contains peony root extract, Polygonatum rhizome / root extract and Gentiana macrophylla extract, and the mass ratio of peony root extract, Polygonatum rhizome / root extract and Gentiana macrophylla extract is 6:4:1.

[0165] This plant composition is prepared by mixing and blending peony root extract, Solomon's seal rhizome / root extract and Gentiana macrophylla extract in a specified ratio.

[0166] Example 2

[0167] This example provides a plant composition with anti-inflammatory and soothing effects and its preparation method. The plant composition contains peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract, and the mass ratio of peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract is 5.8:4.2:1.

[0168] This plant composition is prepared by mixing and blending peony root extract, Solomon's seal rhizome / root extract and Gentiana macrophylla extract in a specified ratio.

[0169] Example 3

[0170] This example provides a plant composition with anti-inflammatory and soothing effects and its preparation method. The plant composition contains peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract, and the mass ratio of peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract is 6.2:3.8:1.

[0171] This plant composition is prepared by mixing and blending peony root extract, Solomon's seal rhizome / root extract and Gentiana macrophylla extract in a specified ratio.

[0172] Example 4

[0173] This example provides a plant composition with anti-inflammatory and soothing effects and its preparation method. The plant composition contains peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract, and the mass ratio of peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract is 5.5:4.5:1.

[0174] This plant composition is prepared by mixing and blending peony root extract, Solomon's seal rhizome / root extract and Gentiana macrophylla extract in a specified ratio.

[0175] Example 5

[0176] This example provides a plant composition with anti-inflammatory and soothing effects and its preparation method. The plant composition contains peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract, and the mass ratio of peony root extract, Polygonatum odoratum rhizome / root extract and Gentiana macrophylla extract is 6.5:3.5:1.

[0177] This plant composition is prepared by mixing and blending peony root extract, Solomon's seal rhizome / root extract and Gentiana macrophylla extract in a specified ratio.

[0178] Comparative Example 1

[0179] This comparative sample provides a single peony root extract.

[0180] Comparative Example 2

[0181] This comparative example provides a single rhizome / root extract of Polygonatum odoratum.

[0182] Comparative Example 3

[0183] This comparative example provides a single Gentiana macrophylla extract.

[0184] Comparative Example 4

[0185] This example provides a plant composition and its preparation method, which is basically the same as in Example 1, except that the mass ratio of peony root extract, Polygonatum rhizome / root extract and Gentiana macrophylla extract is 4:6:1.

[0186] Comparative Example 5

[0187] This example provides a plant composition and its preparation method, which is basically the same as in Example 1, except that the mass ratio of peony root extract, Polygonatum rhizome / root extract and Gentiana macrophylla extract is 1:4:6.

[0188] Comparative Example 6

[0189] This example provides a plant composition and its preparation method, which is basically the same as in Example 1, except that the mass ratio of peony root extract, Polygonatum rhizome / root extract and Gentiana macrophylla extract is 1:1:1.

[0190] Comparative Example 7

[0191] This example provides a plant composition and its preparation method, which is basically the same as in Example 1, except that: the peony root extract is replaced with an equal amount of purslane extract, and the mass ratio of purslane extract, Polygonatum rhizome / root extract and Gentiana macrophylla extract is 6:4:1.

[0192] Comparative Example 8

[0193] This example provides a plant composition and its preparation method, which is basically the same as in Example 1, except that the plant composition contains peony root extract and gentian root extract, and the mass ratio of peony root extract to gentian root extract is 5.5:1.

[0194] Application Example 1

[0195] This example provides a face cream containing water, panthenol, 1,2-hexanediol, p-hydroxyacetophenone, sodium stearoyl glutamate, ammonium acryloyl dimethyl taurate / VP copolymer, the plant composition with anti-inflammatory and soothing effects of Example 1, polyglycerol-3-methylglucose distearate, rice bran oil, and phytosterol isostearyl dioleate. The specific ingredient ratios are shown in Table 7.

[0196] The preparation method of this face cream includes: mixing each ingredient according to the formula raw materials, and stirring at a stirring speed of 1300±200rpm for 10min to make it evenly.

[0197] Application Example 2

[0198] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the anti-inflammatory and soothing plant composition of Example 2.

[0199] Application Example 3

[0200] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the anti-inflammatory and soothing plant composition of Example 3.

[0201] Application Example 4

[0202] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the anti-inflammatory and soothing plant composition of Example 4.

[0203] Application Example 5

[0204] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the anti-inflammatory and soothing plant composition of Example 5.

[0205] Application Comparative Example 1

[0206] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the plant composition of Comparative Example 4.

[0207] Application Comparative Example 2

[0208] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the plant composition of Comparative Example 5.

[0209] Application Comparative Example 3

[0210] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the plant composition of Comparative Example 6.

[0211] Application Comparative Example 4

[0212] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the plant composition of Comparative Example 7.

[0213] Application Comparative Example 5

[0214] This example provides a face cream and its preparation method, which is basically the same as in Example 1, except that the anti-inflammatory and soothing plant composition of Example 1 is replaced with the plant composition of Comparative Example 8.

[0215] Table 7 Face Cream Recipes

[0216]

[0217] The purslane extract mentioned in the comparative examples was purchased from Huiwen Biotechnology Co., Ltd. Acryloyl dimethyl taurate ammonium / VP copolymer was purchased from CLARIANT, brand name Aristoflex AVC; polyglycerol-3-methyl glucoside distearate was purchased from EVONIK, brand name... Care 450MB; rice bran oil, purchased from Shanghai Kerry Oils & Grains Industry Co., Ltd., brand name Lijing Rice Bran Oil; phytosterol isostearyl dioleate, purchased from Shanghai Huiwen Biotechnology Co., Ltd., brand name H-PID.

[0218] Performance testing

[0219] (I) Safety performance testing:

[0220] This experiment used a patch test to verify the safety of the plant composition in this invention.

[0221] Evaluation method:

[0222] Subjects (average age 25 years, 24-27 years) underwent skin patch testing on their forearms using Finn Chambers. The test sites were first wiped with 70% ethanol and dried. Then, 25-30 μL of test sample was added to the Finn Chamber, which was then fixed in place over the forearm area, ensuring it remained dry. The patch was removed after 24 hours. The test sites were marked with a marker, and observed again at 30 minutes and 24 hours, with detailed information recorded from the testers. The degree of irritation was classified according to the International Contact Dermatitis Research Group (ICDRG) criteria. The assessment was conducted 30 minutes after patch removal and again after 24 hours. Finally, the skin reaction was assessed according to the International Association for the Study of Contact Dermatitis's criteria (Table 8) to determine whether irritation was present.

[0223] Table 8. Criteria for Skin Reaction by the International Association for the Study of Contact Dermatitis

[0224]

[0225] The test results are as follows:

[0226] Table 9 Results of Skin Patch Test

[0227]

[0228]

[0229] The results showed that the plant composition Tri-AS01 (Gentiana macrophylla + Polygonatum sibiricum + Paeonia lactiflora) of the present invention was non-irritating.

[0230] (II) Anti-inflammatory test

[0231] The ELISA kit was used to evaluate the anti-inflammatory effect of the Tri-AS01 plant composition on mouse monocytes / macrophages (RAW264.7) by detecting IL-6 concentration. The detection method is as follows:

[0232] (1) Cell inoculation

[0233] After passaged RAW264.7 cells to the third generation, once the cells had confined the culture flask to a single layer (70%–80%), they were then injected with 1×10⁻⁶ cells per cell line. 5Inoculate 1 mL per well into 24-well plates at a concentration of 1 mL / mL and incubate in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0234] (2) Cell treatment methods

[0235] Remove the culture medium and add 500 μL of sample solution diluted with culture medium. Divide the samples into three groups: a blank control group (cell culture medium treatment), a cell inflammation model group (20 μg / mL LPS treatment), and a sample group (2% Gentiana macrophylla, 2% Polygonatum sibiricum, 2% Paeonia lactiflora, 2% Tri-AS01 + 20 μg / mL LPS treatment). Continue incubation for 24 h. Centrifuge the culture supernatant for 3 min (4℃, 15000 rpm / min), and determine the IL-6 content in the supernatant according to the ELISA kit instructions.

[0236] Note: The plant composition made from peony root extract, Polygonatum rhizome / root extract and Gentiana macrophylla extract in Example 1 is abbreviated as Tri-AS01; peony root extract is abbreviated as peony, Polygonatum rhizome / root extract is abbreviated as Polygonatum, and Gentiana macrophylla extract is abbreviated as Gentiana macrophylla.

[0237] (3) Data Analysis

[0238] All experiments were repeated three times. Results are expressed as mean ± standard deviation (SD). Data were analyzed statistically using one-way ANOVA. A p-value < 0.05 was considered statistically significant.

[0239] The results are shown in Table 10 and... Figure 5 As shown.

[0240] Table 10 Inhibitory effect on IL-6

[0241]

[0242] Note: *P<0.05.

[0243] The experimental results showed that, compared with the blank control group, LPS treatment significantly upregulated the expression of the inflammatory cytokine IL-6 in mouse monocytes and macrophages. Treatment with the same concentration of extracts also showed good inhibitory effects on the IL-6 inflammatory cytokine. With the same 2% addition, the inhibition rate of IL-6 by Gentiana macrophylla was 81.17%, that of Polygonatum sibiricum was 68.9%, and that of Paeonia lactiflora was 79.82%. The 2% addition resulted in an inhibition rate of 63.92% for Comparative Example 8, 81.39% for Comparative Example 7, 69.28% for Comparative Example 6, 51.55% for Comparative Example 5, and 83.28% for Comparative Example 4. The plant composition Tri-AS01 (Gentiana macrophylla + Polygonatum sibiricum + Paeonia lactiflora) of this invention showed an inhibition rate of 93.3%, demonstrating that the specific ratio of these compounds resulted in a better inhibitory effect on the inflammatory cytokine IL-6.

[0244] (III) Facial Soothing Effect Improvement Test

[0245] The face cream samples prepared in Application Example 1 and Application Comparative Example 5 were distributed to the subjects, who were instructed to apply them evenly to their faces twice a day, morning and evening, and to conduct tests according to the following methods:

[0246] Several volunteers aged 18-45 were selected. On the day of the test, after cleansing their faces, the samples were left to rest for 30 minutes in a constant temperature and humidity environment. The Visia 7 instrument was used to measure facial erythema images and a* values ​​before use, immediately after use (30 minutes), 7 days after use, and 14 days after use for evaluation. The a* value, derived from the L*a*b* color space system, represents the color components from green to red. A higher value indicates a more pronounced degree of skin erythema, thus serving as an important indicator of skin inflammation and changes in erythema. Therefore, a decrease in the a* value directly reflects an improvement in the skin erythema condition. The improvement is expressed as the improvement rate of each value, calculated as follows:

[0247] Improvement rate of facial redness = (facial a* value after using the test sample - facial a* value before using the test sample) / facial a* value before using the test sample × 100%.

[0248] The test results and the calculated average data are shown in Table 11 below;

[0249] Table 11 Results of Improvement Rate for Facial Redness

[0250]

[0251]

[0252] The results above show that the test samples containing the plant composition provided by this invention exhibit better soothing effects. Specifically, the a* values ​​of the test cream samples prepared using the plant composition provided by this invention show improvement rates of 11.5% immediately after use (30 min), 18.8% after 7 days, and 25.4% after 14 days. The observation figures before and after using Example 1 are shown below. Figure 6 As shown, from Figure 6 It can also be clearly seen that the plant composition provided by the present invention can effectively improve facial redness, thereby achieving a soothing effect.

[0253] Furthermore, this invention utilizes the synergistic effect of plant extracts to reduce irritation while ensuring highly effective anti-inflammatory results, making it suitable for a wider range of skin types, especially sensitive skin. Simultaneously, by using natural plant ingredients instead of synthetic hormones, it avoids the potential long-term safety issues associated with synthetic hormones, ensuring the product's safety and suitability for long-term use.

[0254] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly. They should not be construed as limiting the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

[0255] The endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

Claims

1. A plant composition with anti-inflammatory and soothing effects, characterized in that, The plant composition comprises peony root extract, Polygonatum odoratum rhizome / root extract, and Gentiana macrophylla extract.

2. The plant composition with anti-inflammatory and soothing effects according to claim 1, characterized in that, The mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5-9:2-5:

1.

3. The plant composition with anti-inflammatory and soothing effects according to claim 2, characterized in that, The mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5-7:3-4.5:

1.

4. The plant composition with anti-inflammatory and soothing effects according to claim 3, characterized in that, The mass ratio of the peony root extract, the Polygonatum multiflorum rhizome / root extract, and the Gentiana macrophylla extract is 5.5-6.5:3.5-4.5:

1.

5. The plant composition with anti-inflammatory and soothing effects according to any one of claims 1-4, characterized in that, The peony root extract is prepared by the following method: peony root powder is extracted using an aqueous alcohol solvent as the solvent, the extract is collected and purified, and then dried.

6. The plant composition with anti-inflammatory and soothing effects according to any one of claims 1-4, characterized in that, The rhizome / root extract of Polygonatum multiflorum was prepared by the following method: The washed rhizomes / roots of Polygonatum odoratum are steamed and dried repeatedly until they turn black, thus producing Polygonatum odoratum powder. Water was used as a solvent to extract Polygonatum odoratum powder by heating. The extract was concentrated and then an alcohol solvent or its aqueous solution was added to precipitate the crude product. The crude product was dispersed in a mixture of water, chlorinated alkanes and alcohol, allowed to stand and separate into layers, and the supernatant was collected. Add an alcohol solvent to the supernatant, precipitate, and dry the precipitate.

7. The plant composition with anti-inflammatory and soothing effects according to any one of claims 1-4, characterized in that, The Gentiana macrophylla extract was prepared by the following method: Gentiana macrophylla powder was extracted by heating with water as solvent, the extract was collected and purified, and then dried.

8. A method for preparing a plant composition with anti-inflammatory and soothing effects as described in any one of claims 1-7, characterized in that, The preparation method includes: mixing and blending the various components to obtain a plant composition.

9. The use of a plant composition having anti-inflammatory and soothing effects as described in any one of claims 1-7 in the preparation of a cosmetic having anti-inflammatory and soothing effects.

10. The application according to claim 9, characterized in that, The plant composition comprises 0.1%-5% of the cosmetic product by weight, and more particularly 0.5%-4%.

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