Royal jelly nourishing and moisturizing face cream and preparation method thereof

Abstract

The invention discloses a royal jelly nourishing and moisturizing face cream and a preparation method thereof, and belongs to the technical field of cosmetics, the face cream comprises the following components: phase A: pure water, a natural moisturizer, water-soluble active polysaccharide and the like; the B phase comprises a grease matrix, an emulsifier, a consistency regulator and the like, and the C phase comprises fresh royal jelly and nano blue thistle oil NLC, and the royal jelly face cream is prepared through synergism of all phase components and combination of processes such as step-by-step temperature control, gradient stirring, high-speed homogenization and vacuum defoaming, so that the problems of poor component absorption and thick and sticky texture of the traditional royal jelly face cream are solved, deep permeation and absorption of effective components are realized, and the skin care effect is improved. The texture is light and adapts to various skin types; meanwhile, the moisturizing power of the skin can be remarkably enhanced, dryness (the improvement rate is 55.61%) and desquamation (the improvement rate is 63.45%) are improved, the skin elasticity (the improvement rate is 36.88%) and the glossiness (the improvement rate is 30.50%) are improved, and the skin care product is mild and free of irritation and has excellent nourishing and moisturizing effects and good use experience.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a royal jelly nourishing and moisturizing face cream and its preparation method. Background Technology

[0002] Royal jelly is rich in proteins, amino acids, vitamins, and other active ingredients. Due to its excellent nourishing and moisturizing effects, it is widely used in moisturizing face creams. However, existing royal jelly moisturizing face creams still suffer from problems in practical applications, such as poor ingredient absorption, unpleasant texture, and difficulty in quality control, which seriously restricts their efficacy and user experience.

[0003] The large molecular components such as active proteins in royal jelly are difficult to penetrate the skin's stratum corneum barrier, resulting in a low actual skin absorption rate, which prevents its core effects such as moisturizing and anti-aging from being fully realized. Even 10-hydroxy-2-decenoic acid (royal jelly acid), which plays a key role in royal jelly, is difficult to achieve deep penetration and efficient absorption under current formulation and processing technology due to insufficient integration with other raw materials.

[0004] Some royal jelly moisturizing creams have a noticeable grainy texture, which directly affects the application experience. In addition, the texture of the cream is often too thick, sticky or oily, and it is not easily accepted by the skin after application. It is only suitable for dry skin. For oily and combination skin, it is easy to cause discomfort such as suffocation and excessive oiliness, which significantly limits the skin type suitability of the product.

[0005] Royal jelly is chemically unstable and easily oxidized and deteriorated by factors such as temperature and light. This means that existing royal jelly products need to be frozen at -18°C. However, the daily storage environment in a home often cannot meet this stringent requirement, causing the product to easily become ineffective before it is opened. At the same time, once opened, due to insufficient sealing and formula stability, bacteria can easily grow, further shortening the product's shelf life and affecting its safety.

[0006] The aforementioned issues prevent the natural advantages of royal jelly from being fully utilized in moisturizing face creams, necessitating a technological solution that addresses these pain points to enhance the product's efficacy, applicability, and stability. Summary of the Invention

[0007] The purpose of this invention is to address the shortcomings of existing technologies by providing a royal jelly nourishing and moisturizing face cream and its preparation method, thereby solving problems such as poor absorption of royal jelly components, unpleasant face cream texture, and difficulty in preservation, and achieving the effects of easy absorption, suitability for various skin types, easy preservation, and significant moisturizing and nourishing effects.

[0008] To solve the above-mentioned technical problems, the present invention provides a royal jelly nourishing and moisturizing face cream, comprising the following components by weight percentage:

[0009] Phase A: 70-72% pure water, 2-3% natural moisturizer, 0.3-0.6% water-soluble active polysaccharide, 0.1% propylparaben, and 0.05% disodium EDTA;

[0010] Phase B: 10-13% oil matrix, 1-1.5% emulsifier, 3-5% consistency regulator, 1-2% vitamin E acetate, 1-2% S170 and 0.2-0.5% methylparaben;

[0011] Phase C: 4-5% fresh royal jelly, 1-2% nano-blue thistle oil (NLC), and 0.05-0.2% plant fragrance.

[0012] This invention's royal jelly nourishing and moisturizing face cream enhances basic moisturizing and penetration through phase A natural moisturizers and water-soluble active polysaccharides, phase B optimizes the oil matrix and emulsification system to improve texture and stability, and phase C combines the synergistic activity of fresh royal jelly and nano-blue thistle oil (NLC). This not only solves the problems of poor absorption and heavy, sticky texture of traditional royal jelly face creams, achieving deep penetration and absorption of active ingredients, but also improves product stability (no need for strict freezing preservation). Its lightweight texture is suitable for various skin types. Simultaneously, it significantly enhances skin hydration, improves dryness and flaking, and enhances skin elasticity and radiance. It is gentle and non-irritating, combining excellent nourishing and moisturizing effects with a pleasant user experience.

[0013] As a further description of the above technical solution, the natural moisturizer includes honey and panthenol, with a weight ratio of honey to panthenol of (9-10):(1-2). Honey (preferably acacia honey) enhances water retention and nourishment with its natural moisturizing factors, while panthenol enhances the longevity of moisturizing by promoting skin barrier repair. The two work synergistically to leverage the deep nourishing advantages of honey and improve the absorption efficiency of water-soluble active polysaccharides and other ingredients through the penetrating assistance of panthenol, further strengthening the basic moisturizing ability of phase A. This, combined with the optimized texture of phase B and the active ingredients of phase C, makes the face cream more targeted in terms of immediate hydration, long-lasting water retention, and improved skin softness, thereby enhancing the overall moisturizing and nourishing efficacy.

[0014] As a further description of the above technical solution, the water-soluble active polysaccharide includes sodium hyaluronate and tremella polysaccharide, with a mass ratio of sodium hyaluronate to tremella polysaccharide of 2:1. The water-soluble active polysaccharide utilizes the complementary and synergistic effects of sodium hyaluronate and tremella polysaccharide: sodium hyaluronate, with its high hydrophilicity, enhances the surface hydration and water-locking ability, while tremella polysaccharide nourishes the stratum corneum and promotes barrier repair, enhancing the long-lasting deep hydration. This approach leverages the immediate hydrating advantage of sodium hyaluronate while reducing the stickiness that high-concentration sodium hyaluronate may cause through the gentle nourishing properties of tremella polysaccharide. Together with phase A natural moisturizers, it enhances the layering of the basic moisturizing system and further assists in the penetration and absorption of phase C royal jelly active ingredients. This makes the cream more efficient in its progressive hydration—hydration-locking-nourishing—significantly improving dry skin and enhancing the overall balance of moisturizing and nourishing.

[0015] As a further description of the above technical solution, the oil matrix includes mineral oil, linseed oil, jojoba seed oil, caprylic / capric triglyceride, hydrogenated polyisobutylene and polydimethylsiloxane, with a mass ratio of (2-3):(2-3):(2-3):(1-2):(1-2):(1-2). Mineral oil provides basic sealing and water-locking capabilities, while flaxseed oil and jojoba seed oil replenish skin lipids and strengthen nourishment through their skin-friendly properties. Caprylic / capric triglycerides and dimethicone adjust the lightweight texture to reduce stickiness, and hydrogenated polyisobutylene enhances the smoothness of the skin feel. This formula, blended in specific proportions, ensures basic moisturizing and nourishing through mineral oil and nourishing oils, while balancing the texture with lightweight oils and silicone oils, avoiding the heaviness of traditional face creams. It is suitable for various skin types, including dry, oily, and combination skin. In addition, it works synergistically with phase A moisturizing ingredients and phase C active ingredients to provide a suitable carrier environment for the penetration and absorption of effective ingredients such as royal jelly, further enhancing the nourishing and moisturizing efficacy of the face cream.

[0016] As a further description of the above technical solution, the emulsifier includes stearyl alcohol polyether and glyceryl stearate, with a mass ratio of stearyl alcohol polyether to glyceryl stearate of 2:3. Stearyl alcohol polyether, with its excellent oil-water emulsification ability, promotes the uniform dispersion of the oil phase matrix and aqueous phase components, enhancing system stability; glyceryl stearate, while strengthening the emulsification effect, moderately thickens and adjusts the cream's texture, reducing graininess. The two are compounded in a specific ratio, synergistically enhancing each other. Stearyl alcohol polyether ensures long-term stability of oil-water dispersion (avoiding layering and sedimentation), while the thickening properties of glyceryl stearate balance the cream's consistency, making it neither too thin nor too thick. Together with the B-phase oil matrix, it optimizes the skin feel, making the cream easier to apply and spread. Simultaneously, it assists in the uniform distribution of the A-phase moisturizing ingredients and the C-phase active components, further improving the penetration efficiency of the effective ingredients and enhancing the overall product stability.

[0017] As a further description of the above technical solution, the stearyl alcohol polyether is at least one of stearyl alcohol polyether-2 and stearyl alcohol polyether-21. Both stearyl alcohol polyether-2 and stearyl alcohol polyether-21 are highly efficient nonionic emulsifiers, which can be flexibly selected or compounded according to the needs of the system: stearyl alcohol polyether-2 has both emulsifying and mild cleansing auxiliary properties, while stearyl alcohol polyether-21 focuses on enhancing the stability of the oil-water interface. When the two work together (or act alone), they can be precisely matched with glyceryl stearate, further improving the emulsification and dispersion efficiency of the oil phase matrix and the aqueous phase components, and enhancing the system's anti-stratification and anti-precipitation capabilities; at the same time, their mild emulsifying properties can reduce irritation to the skin barrier, and work synergistically with the oil matrix to regulate the smoothness of face cream application, avoiding sticky residue, making the texture more suitable for various skin types, and providing a stable carrier for the uniform distribution and penetration absorption of phase A moisturizing ingredients and phase C active components, further enhancing the stability of the product.

[0018] As a further description of the above technical solution, the viscosity modifier includes cetearyl alcohol, stearic acid, and beeswax, with a mass ratio of cetearyl alcohol, stearic acid, and beeswax of 2:1:(0.1-0.5). These three components work synergistically to construct a suitable texture system: cetearyl alcohol serves as the basic thickening framework, ensuring the cream's basic consistency and structural stability; stearic acid enhances the intermolecular forces, further strengthening the cream's smoothness and resistance to deformation; and the low proportion of beeswax, with its natural lubricating properties, adjusts the smoothness of the skin feel without increasing the heaviness. This avoids the stiffness or looseness caused by excessive amounts of a single ingredient, and works synergistically with emulsifiers and oil matrices to make the cream's texture uniform, delicate, and of moderate consistency. It is easy to apply and spread without being too thin, causing the loss of moisturizing ingredients, or too thick, causing a sticky feeling, making it suitable for various skin types. Simultaneously, the stable viscosity structure helps to evenly disperse active ingredients such as C-phase royal jelly, further enhancing the cream's nourishing and moisturizing efficacy.

[0019] The present invention also provides a method for preparing the above-mentioned royal jelly nourishing and moisturizing face cream, comprising the following steps:

[0020] S1. After mixing the B phase raw materials, heat to 80-85℃ and stir for 0.5-1.5 hours until the mixture is uniform;

[0021] S2. Mix pure water and water-soluble active polysaccharide, heat to 50-60℃, stir until the water-soluble active polysaccharide is completely dissolved, and transfer to an emulsifying pot.

[0022] S3. Add the remaining raw material of phase A to the emulsification pot, stir at 40-60 r / min and heat to 80-85℃, and maintain for 20-40 min;

[0023] S4. Transfer the homogenized B phase raw material from step S1 to an emulsifying pot, stir at low speed for 1-3 minutes, then homogenize and emulsify at high speed for 2-5 minutes, and finally defoam under vacuum for 2-4 times while stirring at low speed.

[0024] S5. When the temperature is cooled to 45-55℃, add the C phase raw material, continue stirring and cooling until the temperature drops to 30-40℃, stop stirring, let it stand and stabilize, and then package it to obtain the royal jelly nourishing and moisturizing face cream.

[0025] The preparation method of this invention achieves synergistic effects through precise control of parameters at each stage: First, the B-phase raw material is heated and stirred to ensure uniform dispersion of the oil components, laying the foundation for subsequent emulsification stability; then, the A-phase raw material is specifically treated to promote the full dissolution and mixing of water-soluble components at a specific temperature, ensuring the homogeneity of the aqueous system; next, through a combination of low-speed premixing, high-speed homogenization emulsification (promoting nanoscale fusion of oil and water phases), and vacuum defoaming, the graininess is completely eliminated, the system stability is improved, and the problems of traditional face creams being thick and prone to separation are solved; finally, the C-phase active ingredients are added at a suitable temperature to avoid high temperature damaging the activity of royal jelly, and finally, a stable and homogeneous product is formed after standing. The entire process, through step-by-step temperature control, gradient stirring, and precise emulsification, not only ensures the stability and uniform dispersion of each phase component (especially the active ingredients of royal jelly), but also enhances the penetration and absorption efficiency of the effective ingredients, making the finished product light and delicate in texture, suitable for various skin types, while extending the shelf life and synergistically improving the nourishing and moisturizing effects and the comfort of use.

[0026] As a further description of the above technical solution, the low-speed stirring speed in step S4 is 25-35 r / min, the high-speed homogenization emulsification speed is 2500-3500 r / min, and the vacuum degree of vacuum defoaming is 0.04-0.06 Pa. The 25-35 r / min low-speed stirring gently premixes the oil and water phases, avoiding the introduction of excessive air bubbles from vigorous stirring, thus laying a uniform foundation for subsequent emulsification. The 2500-3500 r / min high-speed homogenization emulsification achieves nanoscale fusion of the oil and water phases through strong shear force, uniformly dispersing active ingredients such as royal jelly in the matrix, significantly improving ingredient compatibility and penetration efficiency, and solving the problems of uneven ingredient dispersion and poor absorption in traditional processes. The 0.04-0.06 Pa vacuum defoaming precisely eliminates air bubbles within the system, preventing voids or a grainy texture, ensuring the cream is delicate and smooth, structurally stable and does not separate, while also facilitating the efficient penetration of royal jelly active ingredients, further enhancing the nourishing and moisturizing effects and user experience.

[0027] As a further description of the above technical solution, the vacuum defoaming in step S4 is performed at intervals of 20-40 seconds, with a cumulative time of 1-2 minutes. Setting the vacuum defoaming interval to 20-40 seconds avoids sudden pressure changes in the system caused by continuous vacuuming, allowing sufficient time for bubbles to rise and aggregate, preventing damage to the nanoscale fusion structure of the oil and water phases due to instantaneous negative pressure impact. The limitation on the cumulative processing time precisely controls the degree of defoaming, ensuring the complete elimination of residual bubbles (avoiding pores and a grainy texture) without causing the sedimentation of active ingredients due to over-processing. While ensuring the stability of the emulsion system, it further enhances the smoothness and fineness of the cream texture, allowing active ingredients such as royal jelly to be more evenly dispersed in the matrix, laying the foundation for the efficient penetration of subsequent active ingredients, and ultimately improving the stability of the product's skin feel and nourishing and moisturizing effects.

[0028] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0029] 1. The royal jelly nourishing and moisturizing face cream of this invention achieves multiple synergistic advantages through a scientifically formulated blend of phase A moisturizing and nourishing components, phase B emulsifying and stabilizing components, and phase C active functional components. The active ingredients of royal jelly penetrate the skin efficiently via a nano-level emulsification system, solving the problem of poor absorption in traditional products. The texture, synergistically regulated by the oil matrix and emulsifiers, is light, delicate, and non-sticky, suitable for various skin types including dry, oily, and combination skin. Simultaneously, the synergistic effect of each component enhances the system's stability, eliminating the need for stringent freezing preservation and extending shelf life. In terms of efficacy, it significantly improves dry, flaky skin, enhances elasticity and radiance, and is gentle and non-irritating, offering both excellent nourishing and moisturizing effects and a comfortable user experience.

[0030] 2. The preparation method of this invention achieves efficient synergy through stepwise temperature control, gradient stirring, and precise process parameters (such as low-speed premixing, high-speed homogenization emulsification, specific vacuum defoaming, and interval time settings). Stepwise processing of each phase of raw materials ensures component stability, while gradient speed and homogenization emulsification promote nanoscale fusion of oil and water phases, solving the problems of uneven component dispersion and rough texture in traditional processes. The control of the interval and duration of vacuum defoaming avoids residual bubbles and system damage, protecting the activity of active ingredients such as royal jelly while enhancing component compatibility and penetration efficiency. Simultaneously, by utilizing nanoemulsification technology and specific preparation processes, 10-hydroxy-2-decenoic acid (royal jelly acid, molecular weight 182 Daltons) in royal jelly is fully integrated with other raw materials, allowing for deep penetration into the skin barrier and absorption by cells, solving the problem of difficult absorption of large molecular components. Furthermore, process optimization improves the stability of royal jelly, eliminating the need for low-temperature freezing and allowing for storage under normal household conditions; it is also less prone to bacterial growth after opening. It completely solves the problems of poor ingredient absorption, poor texture and user experience, and difficulty in preserving royal jelly, helping to stabilize the texture of the finished product, improve its efficacy, and facilitate industrial production. Detailed Implementation

[0031] The claims of the present invention will be further described in detail below with reference to specific embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made by any person within the scope of protection of the claims of the present invention are still within the scope of protection of the claims of the present invention.

[0032] It is worth noting that, unless otherwise specified, all components used in this invention are materials existing in the art and can be obtained through commercial channels or prepared by existing methods.

[0033] Example 1

[0034] This embodiment provides a royal jelly nourishing and moisturizing face cream, comprising the following components by weight percentage:

[0035] Phase A: 71.4% pure water, 2% locust honey, 0.3% panthenol, 0.2% sodium hyaluronate (pure powder, purity ≥99%), 0.1% tremella polysaccharide, 0.1% propylparaben, 0.05% disodium EDTA;

[0036] Phase B: Mineral oil (pharmaceutical grade white mineral oil, viscosity range of 10-20 mm² / s at 40℃) 3%, linseed oil 2%, jojoba seed oil 2.5%, caprylic / capric triglyceride 1.5%, hydrogenated polyisobutylene 2%, polydimethylsiloxane 1%, stearyl alcohol polyether-21 0.3%, stearyl alcohol polyether-2 0.2%, glyceryl stearate 0.75%, cetearyl alcohol 2%, stearic acid 1%, beeswax 0.3%, vitamin E acetate 1%, S170 1.5%, methylparaben 0.4%;

[0037] Phase C: 5% fresh royal jelly (active ingredient 10-hydroxy-2-decenoic acid content ≥1.4%), 1.2% nano-blue thistle oil (NLC), and 0.2% cherry blossom fragrance.

[0038] Its preparation method includes the following steps:

[0039] S1. Add the B phase raw materials to the oil phase pot in sequence, heat to 82°C, and stir for 1 hour until the mixture is uniform.

[0040] S2. Add pure water and water-soluble active polysaccharides to the aqueous phase pot, turn on heating and stirring, raise the temperature to 55°C, stir for about 2 hours until the water-soluble active polysaccharides are completely dissolved, and transfer all the raw materials in the aqueous phase pot to the emulsification pot.

[0041] S3. Add the remaining raw material of phase A to the emulsifying pot, set the speed to 50 r / min, heat to 82℃, and stir for 30 min;

[0042] S4. Transfer the homogenized B phase raw material from step S1 to the emulsifying pot. First, stir at a low speed of 30 r / min for 1 minute, then turn on the homogenizer and set the speed to 3000 r / min for high-speed homogenization and emulsification for 2 minutes. Then, turn off the homogenizer and perform vacuum defoaming 3 times under a low speed of 30 r / min at 0.05 Pa.

[0043] S5. Turn on the cooling water and cool under low speed stirring at 30r / min. When the temperature drops to 50℃, stop stirring, add the C phase raw material, continue stirring and cooling until the temperature drops to 35℃, stop stirring, remove from the pot, and let stand for 24 hours to stabilize before packaging (50g / bottle) to obtain the royal jelly nourishing and moisturizing face cream.

[0044] Example 2

[0045] This embodiment provides a royal jelly nourishing and moisturizing face cream, comprising the following components by weight percentage:

[0046] Phase A: 70% pure water, 2.5% locust honey, 0.5% panthenol, 0.4% sodium hyaluronate (pure powder, purity ≥99%), 0.2% tremella polysaccharide, 0.1% propylparaben, and 0.05% disodium EDTA.

[0047] Phase B: Mineral oil (pharmaceutical grade white mineral oil, viscosity range of 10-20 mm² / s at 40℃) 3%, linseed oil 3%, jojoba seed oil 3%, caprylic / capric triglyceride 1%, hydrogenated polyisobutylene 1%, polydimethylsiloxane 2%, stearyl alcohol polyether-21 0.2%, stearyl alcohol polyether-2 0.2%, glyceryl stearate 0.6%, cetearyl alcohol 2%, stearic acid 1%, beeswax 0.1%, vitamin E acetate 1.4%, S170 1%, methylparaben 0.2%;

[0048] Phase C: 4.5% fresh royal jelly (active ingredient 10-hydroxy-2-decenoic acid content ≥1.4%), 2% nano-blue thistle oil (NLC), and 0.05% rose fragrance.

[0049] Example 3

[0050] This embodiment provides a royal jelly nourishing and moisturizing face cream, comprising the following components by weight percentage:

[0051] Phase A: 72% pure water, 1.8% locust honey, 0.2% panthenol, 0.2% sodium hyaluronate (pure powder, purity ≥99%), 0.1% tremella polysaccharide, 0.1% propylparaben, 0.05% disodium EDTA;

[0052] Phase B: Mineral oil (pharmaceutical grade white mineral oil, viscosity range of 10-20 mm² / s at 40℃) 2%, linseed oil 2%, jojoba seed oil 2%, caprylic / capric triglyceride 2%, hydrogenated polyisobutylene 1%, polydimethylsiloxane 1%, stearyl alcohol polyether-21 0.3%, stearyl alcohol polyether-2 0.3%, glyceryl stearate 0.9%, cetearyl alcohol 3%, stearic acid 1.5%, beeswax 0.5%, vitamin E acetate 1.5%, S170 2%, methylparaben 0.5%;

[0053] Phase C: 4% fresh royal jelly (active ingredient 10-hydroxy-2-decenoic acid content ≥1.4%), 1% nano-blue thistle oil (NLC), and 0.05% gardenia fragrance.

[0054] Comparative Example 1

[0055] This comparative example provides a royal jelly nourishing and moisturizing face cream, comprising the following components by weight percentage:

[0056] Phase A: 72.6% pure water, 2% locust honey, 0.3% panthenol, 0.2% sodium hyaluronate (pure powder, purity ≥99%), 0.1% tremella polysaccharide, 0.1% propylparaben, 0.05% disodium EDTA;

[0057] Phase B: Mineral oil (pharmaceutical grade white mineral oil, viscosity range of 10-20 mm² / s at 40℃) 3%, linseed oil 2%, jojoba seed oil 2.5%, caprylic / capric triglyceride 1.5%, hydrogenated polyisobutylene 2%, polydimethylsiloxane 1%, stearyl alcohol polyether-21 0.3%, stearyl alcohol polyether-2 0.2%, glyceryl stearate 0.75%, cetearyl alcohol 2%, stearic acid 1%, beeswax 0.3%, vitamin E acetate 1%, S170 1.5%, methylparaben 0.4%;

[0058] Phase C: 5% fresh royal jelly (active ingredient 10-hydroxy-2-decenoic acid content ≥1.4%), 0.2% cherry blossom fragrance.

[0059] Comparative Example 2

[0060] This comparative example provides a royal jelly nourishing and moisturizing face cream, comprising the following components by weight percentage:

[0061] Phase A: 71.4% pure water, 2% locust honey, 0.3% panthenol, 0.2% sodium hyaluronate (pure powder, purity ≥99%), 0.1% tremella polysaccharide, 0.1% propylparaben, 0.05% disodium EDTA;

[0062] Phase B: Mineral oil (pharmaceutical grade white mineral oil, viscosity range of 10-20 mm² / s at 40℃) 3%, linseed oil 2%, jojoba seed oil 2.5%, caprylic / capric triglyceride 1.5%, hydrogenated polyisobutylene 2%, polydimethylsiloxane 1%, stearyl alcohol polyether-21 0.3%, stearyl alcohol polyether-2 0.2%, glyceryl stearate 0.75%, cetearyl alcohol 2%, stearic acid 1%, beeswax 0.3%, vitamin E acetate 1%, S170 1.5%, methylparaben 0.4%;

[0063] Phase C: 5% fresh royal jelly (active ingredient 10-hydroxy-2-decenoic acid content ≥1.4%), 1.2% common blue thistle oil (NLC), and 0.2% cherry blossom fragrance.

[0064] Efficacy test:

[0065] To verify the actual efficacy of the royal jelly nourishing and moisturizing face cream of the present invention, human efficacy evaluation tests were conducted on the face creams of Example 1, Comparative Example 1, and Comparative Example 2, as detailed below:

[0066] Test sample

[0067] Royal jelly nourishing and moisturizing face creams prepared in Example 1, Comparative Example 1, and Comparative Example 2.

[0068] Subject selection

[0069] Healthy subjects aged 20–55 years with dry, loose, and dull skin, no chronic diseases or skin conditions, who voluntarily signed informed consent and complied with the testing requirements were selected. Subjects who had used antihistamines within the past week, immunosuppressants within the past month, or anti-inflammatory drugs at the test site within the past two months, or who had inflammatory skin diseases, diabetes, asthma, etc., were excluded. Other subjects included breastfeeding / pregnant women, those with scars / pigmentation abnormalities at the test site that might affect the results, and those participating in other clinical trials were also excluded. Ninety-three eligible subjects (aged 30–54 years, mean age 44.16 ± 7.79 years) were selected and randomly divided into three groups: Control Group 1, Control Group 2, and the experimental group, with 31 subjects in each group. All subjects completed the 14-day test without any withdrawals.

[0070] Test environment and cycle

[0071] Temperature 21±1℃, humidity 50±10%RH, test period 14 days.

[0072] Testing methods and procedures

[0073] Instructions for use: After cleansing their face in the morning and evening, subjects should take an appropriate amount of face cream (control group 1 uses face cream of Comparative Example 1, control group 2 uses face cream of Comparative Example 2, and experimental group uses face cream of Example 1) and apply it to the cheeks, forehead, chin, and tip of the nose. Then, spread the face cream from the center of the forehead outwards and gently massage in circular motions to help the face cream absorb. Use once in the morning and once in the evening.

[0074] Test method:

[0075] For nourishing efficacy, refer to T / CAFFCI 68-2023, the testing method for nourishing efficacy in cosmetics.

[0076] For moisturizing effects and gentle and non-irritating properties, refer to "Liu Weiyi, Zhou Lin, Zhao Hua. Evaluation of cosmetic efficacy (XII) - Consumer use test [J]. Daily Chemical Industry, 2021, 51(06):485-490";

[0077] Cosmetic Safety Technical Specifications (2015 Edition), Cosmetic Classification Rules and Classification Catalog, and Cosmetic Efficacy Claim Evaluation Specifications.

[0078] Test process:

[0079] Before use (D0): Subjects register their information and sign an informed consent form; laboratory technicians screen subjects' skin types according to inclusion and exclusion criteria; subjects cleanse their faces with the designated facial cleanser and sit quietly in the test environment for 30 minutes; subjects' baseline skin values ​​are tested (Cutometer dual MPA580 measures cheek skin elasticity, Glossymeter GL 200 measures cheek skin radiance); subjects complete a self-assessment questionnaire (degree of dryness of the entire face, degree of peeling, etc.); samples are collected and usage logs are recorded.

[0080] 14 days after use (D14): Subjects cleansed their faces with the designated facial cleanser and sat quietly in the test environment for 30 minutes; skin data values ​​(skin elasticity, radiance) were tested after 14 days of sample use; a post-use self-assessment questionnaire (full face) was completed, and samples and usage logs were collected; adverse skin reactions were recorded.

[0081] Test indicators and judgment criteria

[0082] Instrument testing indicators: Skin elasticity R2 value (%): The closer the R2 value is to 1, the better the elasticity. The calculated improvement rate is: (value after use - value before use) / value before use × 100%; Skin radiance: The higher the value, the higher the radiance. The calculated improvement rate is: (value after use - value before use) / value before use × 100%.

[0083] Subjective assessment indicators: Skin dryness: scored on a 10-point scale (0 points for no dryness, 9 points for extreme dryness), improvement rate calculated as (pre-use score - post-use score) / pre-use score × 100%; Skin peeling: scored on a 10-point scale (0 points for no peeling, 9 points for severe peeling), improvement rate calculated as above; Subject acceptance: including 19 indicators such as "easily absorbed", "mild and non-irritating", "moisturizing / nourishing effect", the percentage of subjects who accepted the indicators as "somewhat agree" (4 points) and "agree" (5 points).

[0084] Safety indicators: Referencing the "Cosmetic Safety Technical Specifications" (2015 edition), adverse skin reactions were recorded (graded from 0 to 4: grade 0 is no reaction; grade 1 is slight erythema; grade 2 is erythema, infiltration, and papules; grade 3 is erythema, edema, papules, and vesicles; grade 4 is erythema, edema, and bullae).

[0085] Result determination basis

[0086] Nourishing effect: Compared with before use (D0), after 14 days of use (D14), both skin luster and skin elasticity R2 value were significantly improved (P<0.05), and the sample was judged to have nourishing effect; otherwise, the sample was judged not to have nourishing effect.

[0087] Moisturizing efficacy: Compared with before use, after 14 days of use, the subjects' self-assessment scores (skin dryness / skin peeling) showed significant improvement, indicating that the sample had moisturizing efficacy; otherwise, the sample was determined not to have moisturizing efficacy.

[0088] Mild and non-irritating: After 14 days of use, no adverse skin reactions related to the sample were observed in any of the subjects with sensitive skin. 100.00% of the subjects agreed that the sample was mild and non-irritating, and the sample was determined to be mild and non-irritating.

[0089] Test Results

[0090] The results of the instrument-detected skin elasticity R2 value (%) and skin luster on the cheek are shown in Table 1:

[0091] Table 1 Instrument test results

[0092]

[0093] As shown in Table 1, the experimental group demonstrated significantly better improvement in both skin elasticity (R²) and skin radiance compared to control groups 1 and 2. Detailed analysis follows:

[0094] Regarding skin elasticity R² value, the experimental group showed an improvement rate of 36.88% after 14 days of use (P<0.001, highly significant difference), with a positive improvement rate of 96.77%. Control group 1 showed an improvement rate of 18.44% (P<0.05, significant difference), with a positive improvement rate of 80.00%. Control group 2 showed an improvement rate of 23.96% (P<0.01, significant difference), with a positive improvement rate of 86.67%. It is evident that the experimental group showed a significantly higher improvement in skin elasticity than the two control groups, and the vast majority of subjects (96.77%) experienced improved elasticity, indicating that it is more effective in enhancing skin elasticity.

[0095] Regarding skin radiance, the experimental group showed an improvement rate of 30.50% (P<0.001, highly significant difference) and a positive improvement rate of 100%; control group 1 showed an improvement rate of 12.48% (P<0.05, significant difference) and a positive improvement rate of 83.33%; and control group 2 showed an improvement rate of 19.21% (P<0.01, significant difference) and a positive improvement rate of 90.00%. The experimental group not only showed the greatest improvement in radiance, but all subjects also observed improvement in radiance, indicating that its effect on improving skin radiance is more comprehensive and significant.

[0096] The subjective evaluation results of the subjects are shown in Table 2. The experimental group showed significantly better results than control groups 1 and 2 in improving both skin dryness and flaking. Regarding skin dryness, the experimental group showed a significantly greater improvement than the two control groups, indicating its superior moisturizing efficacy in relieving skin dryness. Similarly, the experimental group showed significantly better improvement in flaking than the control groups, further confirming its advantages in deep moisturizing and repairing the skin barrier. Therefore, the experimental group outperformed control group 1 (lacking nano-blue thistle oil NLC) and control group 2 (using ordinary blue thistle oil NLC) in both the magnitude and statistical significance of improvement in skin dryness and flaking, demonstrating that the addition of nano-blue thistle oil NLC effectively enhances the product's moisturizing efficacy.

[0097] Table 2 Subjective Evaluation Results

[0098]

[0099] The results of the subjects' agreement evaluation are shown in Table 3. Among them, the evaluation index (feelings after using the sample-1) is: 1 point indicates disagreement, 2 points indicates partial disagreement, 3 points indicates neither disagreement nor agreement, 4 points indicates partial agreement, and 5 points indicates agreement; D14 agreement is the sum of the percentages of "4 points" and "5 points"; the evaluation index (feelings after using the sample-2) is: 1 point indicates "yes", 2 points indicates "no", and D14 agreement is the percentage of "1 point is yes".

[0100] Table 3. Results of Subjects' Acceptance Evaluation

[0101]

[0102] The experimental group showed significantly higher acceptance rates across all evaluation indicators than control groups 1 and 2. Specifically, the experimental group achieved 100.00% acceptance rates for core indicators such as "pleasant skin feel," "easy absorption," and "acceptance of the nourishing effect," as well as 100.00% for "overall acceptance of the trial sample." Control group 1, lacking nano-blue thistle oil NLC, generally had lower acceptance rates across all indicators, particularly for "pleasant skin feel" and "nourishing effect," with acceptance rates below 60%, reflecting poor ingredient absorption and weak perceived efficacy. Control group 2, using ordinary blue thistle oil NLC, had a higher acceptance rate than control group 1, but still lower than the experimental group, highlighting the crucial role of nanoscale carriers in enhancing the product's user experience and perceived efficacy. Furthermore, all three groups showed high acceptance rates (>96.00%) for "mild and non-irritating trial sample," indicating that all three samples were mild and non-irritating.

[0103] Safety evaluation results: After 14 days of use of the sample by the three groups of subjects, the skin reaction in the observation area was grade 0 (no adverse reaction), and no irritation symptoms such as erythema or papules appeared. This meets the evaluation standard for adverse skin reaction in human trials in the "Cosmetic Safety Technical Specifications" (2015 edition) (grade 0 means no reaction).

[0104] Conclusion: Compared with before use, after 14 days of using the royal jelly nourishing and moisturizing face cream of the present invention, the skin elasticity R2 value was significantly improved (P<0.05), with an improvement rate of 36.88%, and the skin radiance was significantly improved (P<0.05), with an improvement rate of 30.50%, proving that the royal jelly nourishing and moisturizing face cream of the present invention has nourishing effects; the subjects' self-assessed skin dryness and skin peeling were significantly improved (P<0.05), with improvement rates of 55.61% and 63.45%, respectively, proving that the royal jelly nourishing and moisturizing face cream of the present invention has moisturizing effects; no adverse skin reactions related to the sample were observed in any subjects, and 100.00% of the subjects agreed that the sample was mild and non-irritating. The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it; those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the present invention.

Claims

1. A royal jelly nourishing and moisturizing face cream, characterized in that, Includes the following components by weight percentage: Phase A: 70-72% pure water, 2-3% natural moisturizer, 0.3-0.6% water-soluble active polysaccharide, 0.1% propylparaben, and 0.05% disodium EDTA; Phase B: 10-13% oil matrix, 1-1.5% emulsifier, 3-5% consistency regulator, 1-2% vitamin E acetate, 1-2% S170 and 0.2-0.5% methylparaben; Phase C: 4-5% fresh royal jelly, 1-2% nano-blue thistle oil (NLC), and 0.05-0.2% plant fragrance.

2. The royal jelly nourishing and moisturizing face cream according to claim 1, characterized in that: The natural moisturizers mentioned include honey and panthenol, with the weight ratio of honey to panthenol being (9-10):(1-2).

3. The royal jelly nourishing and moisturizing face cream according to claim 1, characterized in that: The water-soluble active polysaccharide includes sodium hyaluronate and tremella polysaccharide, with a mass ratio of sodium hyaluronate to tremella polysaccharide of 2:

1.

4. The royal jelly nourishing and moisturizing face cream according to claim 1, characterized in that: The oil matrix includes mineral oil, linseed oil, jojoba seed oil, caprylic / capric triglyceride, hydrogenated polyisobutylene, and polydimethylsiloxane. The mass ratio of mineral oil, linseed oil, jojoba seed oil, caprylic / capric triglyceride, hydrogenated polyisobutylene, and polydimethylsiloxane is (2-3):(2-3):(2-3):(1-2):(1-2):(1-2).

5. The royal jelly nourishing and moisturizing face cream according to claim 1, characterized in that: The emulsifiers include stearyl alcohol polyether and glyceryl stearate, with a mass ratio of stearyl alcohol polyether to glyceryl stearate of 2:

3.

6. The royal jelly nourishing and moisturizing face cream according to claim 5, characterized in that: The stearyl alcohol polyether is at least one of stearyl alcohol polyether-2 and stearyl alcohol polyether-21.

7. The royal jelly nourishing and moisturizing face cream according to claim 1, characterized in that: The consistency modifiers include cetearyl alcohol, stearic acid and beeswax, with the mass ratio of cetearyl alcohol, stearic acid and beeswax being 2:1:(0.1~0.5).

8. The method for preparing the royal jelly nourishing and moisturizing face cream according to any one of claims 1-7, characterized in that, Includes the following steps: S1. Mix the B phase raw materials and heat to 80-85℃, stirring until the mixture is uniform; S2. Mix pure water and water-soluble active polysaccharide, heat to 50-60℃, stir until the water-soluble active polysaccharide is completely dissolved, and transfer to an emulsifying pot. S3. Add the remaining raw material of phase A to the emulsification pot, stir at 40-60 r / min and heat to 80-85℃, and maintain for 20-40 min; S4. Transfer the homogenized B phase raw material from step S1 to an emulsifying pot, stir at low speed for 1-3 minutes, then homogenize and emulsify at high speed for 2-5 minutes, and finally defoam under vacuum for 2-4 times while stirring at low speed. S5. When the temperature is cooled to 45-55℃, add the C phase raw material, continue stirring and cooling until the temperature drops to 30-40℃, stop stirring, let it stand and stabilize, and then package it to obtain the royal jelly nourishing and moisturizing face cream.

9. The method for preparing the royal jelly nourishing and moisturizing face cream according to claim 8, characterized in that: The low-speed stirring speed in step S4 is 25-35 r / min, the high-speed homogenization emulsification speed is 2500-3500 r / min, and the vacuum degree of vacuum defoaming is 0.04-0.06 Pa.

10. The method for preparing the royal jelly nourishing and moisturizing face cream according to claim 8 or 9, characterized in that: The vacuum defoaming process described in step S4 is performed at intervals of 20 to 40 seconds, with a total duration of 1 to 2 minutes.

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