96-hole enzyme-labeled plate washing device
By designing a 96-well ELISA plate washing device with a moving mechanism and an electromagnet ring, the problems of incomplete cleaning and time-consuming processes in existing technologies have been solved, achieving efficient and thorough cleaning of ELISA plates and improving cleaning efficiency and ease of use.
Patent Information
- Application Number
- CN202511473927.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-15
- Publication Date
- 2025-11-14
AI Technical Summary
In the existing technology, the 96-well enzyme-labeled plate washing device does not clean thoroughly and takes a long time when changing the washing solution, resulting in low cleaning efficiency.
A first moving mechanism is designed, comprising a moving plate, a moving block, a mounting groove, a fixed plate, a slide, a slider, a first motor, a first gear, and a first toothed plate, and a second moving mechanism comprising a rotating seat, a rotating shaft, a second motor, a first bevel gear, a second bevel gear, a fixed bar, a second gear, and a second toothed plate. In conjunction with a hydraulic cylinder and an air pump, the mechanism enables the movement of the connector head in different positions and the rapid replacement of the suction head. Waste suction heads are collected using an electromagnet and an iron ring.
It achieves efficient cleaning of 96-well microplates, is easy to use, cleans thoroughly, reduces cleaning time and manual operation, and improves cleaning efficiency.
Smart Images
Figure CN120940303A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of enzyme-labeled plate cleaning technology, and more particularly to a 96-well enzyme-labeled plate washing device. Background Technology
[0002] Currently, there are generally two methods for washing plates in ELISA assays in laboratories. One is the traditional manual washing method, which involves inserting a multi-channel pipette tip, aspirating liquid, injecting it into the ELISA plate, draining the liquid, and patting it dry. This process is repeated until the required number of washes is completed. This method is slow and laborious when washing a large number of plates. The other method is automated washing using an ELISA plate washer. The prepared washing solution is poured into a washing bottle, the washing program is set, and the washing solution is injected into the ELISA plate through a washing tube and a washing head. The plate is shaken to mix, the washing head is inserted into the ELISA plate, and waste liquid is aspirated and discharged into a waste bottle through a waste liquid tube. This process is repeated until the required number of washes is completed. This method of washing plates is fast and labor-saving, but changing the washing solution is relatively troublesome. This is because the washing solution bottle of the enzyme-labeled plate washer is directly connected to the washing head through the washing solution hose, and the washing head directly sucks the waste liquid into the waste liquid tank through the waste liquid hose. When it is necessary to change the washing solution, the washing solution bottle and washing solution hose need to be thoroughly cleaned with clean water, which poses a risk of incomplete cleaning and takes a long time.
[0003] Therefore, it is necessary to provide a new 96-well enzyme-labeled plate washing device to solve the above-mentioned technical problems. Summary of the Invention
[0004] The technical problem solved by this invention is to provide a 96-well enzyme label washing device that is easy to use, has high cleaning efficiency, and cleans thoroughly.
[0005] To solve the above technical problems, the microplate washing device for enzyme labeling provided by the present invention includes: a base plate, two side plates symmetrically fixedly installed on the top of the base plate, a first guide strip fixedly installed on the side of the two side plates that are close to each other, a first moving mechanism installed between the two first guide strips, a hydraulic cylinder installed on the first moving mechanism, a connecting plate fixedly installed on the bottom of the hydraulic cylinder, a plurality of rectangularly distributed connectors fixedly installed on the bottom of the connecting plate, and a second moving mechanism provided on the first moving mechanism, the second moving mechanism being connected to the two first guide strips; The base plate is provided with a mounting plate on top, and the mounting plate is provided with a washing solution tank, at least one clean pipette tip box, a shaking device, and an exhaust pipette tip bucket on top. The shaking device is provided with an enzyme-labeled plate platform.
[0006] Preferably, the first moving mechanism includes a moving plate, with moving blocks fixedly installed on both sides of the moving plate. The moving blocks are slidably installed in the corresponding first guide strips. An installation groove is provided at the bottom of the moving plate, and a fixed plate is slidably installed in the installation groove. The hydraulic cylinder is fixedly installed at the bottom of the fixed plate, and a first motor is fixedly installed at the top of the fixed plate. A first gear is fixedly installed on the output shaft of the first motor, and a first toothed plate is fixedly installed on the inner wall of one side of the installation groove. The first gear meshes with the first toothed plate.
[0007] Preferably, sliding grooves are provided on both inner walls of the mounting groove, and sliders are fixedly installed on both sides of the fixing plate, with the sliders slidably installed in the sliding grooves.
[0008] Preferably, the second moving mechanism includes a rotating shaft rotatably mounted on the top of the moving plate, with second gears fixedly mounted at both ends of the rotating shaft. Below the second gears, a fixing strip is fixedly mounted on the top of the first guide strip. A second toothed plate is fixedly mounted on the top of the fixing strip. The second gear meshes with the second toothed plate. A second motor is fixedly mounted on the top of the moving plate and is connected to the rotating shaft.
[0009] Preferably, a first bevel gear is fixedly mounted on the output shaft of the second motor, and a second bevel gear is fixedly sleeved on the outer side of the rotating shaft, the second bevel gear meshing with the first bevel gear.
[0010] Preferably, the connecting plate is hollow, the connector is connected to the connecting plate, and an air pump is fixedly installed on the top of the connecting plate, with the air inlet end of the air pump connected to the connecting plate.
[0011] Preferably, a plurality of electromagnets are fixedly installed at the bottom of the connecting plate, the electromagnets are sleeved on the outside of the connector, and an iron ring is provided at the bottom of the electromagnet, the iron ring being sleeved on the outside of the connector.
[0012] Compared with related technologies, the microplate washing device for enzyme labeling provided by the present invention has the following advantages: This invention provides a microplate washing device for enzyme-linked immunosorbent assay (ELISA) plates. Through a first moving mechanism consisting of a moving plate, a moving block, a mounting groove, a fixed plate, a sliding groove, a slider, a first motor, a first gear, and a first toothed plate, and a second moving mechanism consisting of a rotating seat, a rotating shaft, a second motor, a first bevel gear, a second bevel gear, a fixed strip, a second gear, and a second toothed plate, the device enables the connector to move at any horizontal position. Simultaneously, in conjunction with a hydraulic cylinder, the connector can move between a clean tip box, an ELISA plate platform, and a waste tip container. An air pump allows the tip to draw the washing solution through the connecting plate and connector. An electromagnet and an iron ring enable the rapid removal of used tips from the connector, thus facilitating the collection of waste tips. Attached Figure Description
[0013] Figure 1 A schematic diagram of a preferred embodiment of the 96-well enzyme-labeled plate washing device provided by the present invention; Figure 2 for Figure 1 The diagram shows a cross-sectional view of the structure. Figure 3 for Figure 1 The diagram shows a top view of the connection structure of the mounting plate, washing tank, clean pipette tip box, shaking device, enzyme-labeled plate platform and waste gas pipette tip container. Figure 4 for Figure 2 The diagram shows a cross-sectional view of the connection structure of the hydraulic cylinder, connecting plate, connector, suction head, air pump, electromagnet, and iron ring. Figure 5 for Figure 2 The enlarged structural diagram of part A is shown.
[0014] The diagram is labeled as follows: 1. Base plate, 2. Side plate, 3. First guide strip, 4. First moving mechanism, 41. Moving plate, 42. Moving block, 43. Mounting groove, 44. Fixing plate, 45. Slide groove, 46. Slider, 47. First motor, 48. First gear, 49. First toothed plate, 5. Suction head, 6. Second moving mechanism, 61. Rotating seat, 62. Rotating shaft, 63. Second motor, 64. First bevel gear, 65. Second bevel gear, 66. Fixing strip, 67. Second gear, 68. Second toothed plate, 7. Reinforcing plate, 8. Hydraulic cylinder, 9. Connecting plate, 10. Connector, 11. Mounting plate, 12. Washing solution tank, 13. Clean suction head box, 14. Shaking device, 15. Enzyme-labeled plate platform, 16. Waste gas suction head container, 17. Air pump, 18. Electromagnet, 19. Iron ring. Detailed Implementation
[0015] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0016] Please refer to the following: Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 ,in, Figure 1 A schematic diagram of a preferred embodiment of the 96-well enzyme-labeled plate washing device provided by the present invention; Figure 2 for Figure 1 The diagram shows a cross-sectional view of the structure. Figure 3 for Figure 1 The diagram shows a top view of the connection structure of the mounting plate, washing tank, clean pipette tip box, shaking device, enzyme-labeled plate platform and waste gas pipette tip container. Figure 4 for Figure 2 The diagram shows a cross-sectional view of the connection structure of the hydraulic cylinder, connecting plate, connector, suction head, air pump, electromagnet, and iron ring. Figure 5 for Figure 2 The diagram shows an enlarged view of part A. The 96-well enzyme-labeled plate washing device includes: a base plate 1, two side plates 2 symmetrically fixedly mounted on the top of the base plate 1, a reinforcing plate 7 fixedly mounted between the two side plates 2, a first guide strip 3 fixedly mounted on the side of the two side plates 2 that are close to each other, a first moving mechanism 4 mounted between the two first guide strips 3, a hydraulic cylinder 8 mounted on the first moving mechanism 4, a connecting plate 9 fixedly mounted on the bottom of the hydraulic cylinder 8, a plurality of rectangularly distributed connectors 10 fixedly mounted on the bottom of the connecting plate 9, and a second moving mechanism 6 provided on the first moving mechanism 4, the second moving mechanism 6 being connected to the two first guide strips 3; The top of the base plate 1 is provided with an installation plate 11. The top of the installation plate 11 is provided with a washing liquid tank 12, at least one clean pipette tip box 13, a shaking device 14, and an exhaust gas pipette tip container 16. The shaking device 14 is provided with an enzyme-labeled plate platform 15. The clean pipette tip box 13 is provided with a pipette tip 5, and the pipette tip 5 is adapted to the connector 10.
[0017] The first moving mechanism 4 includes a moving plate 41, on both sides of which a moving block 42 is fixedly installed. The moving block 42 is slidably installed in the corresponding first guide strip 3. The bottom of the moving plate 41 is provided with a mounting groove 43, and a fixed plate 44 is slidably installed in the mounting groove 43. The hydraulic cylinder 8 is fixedly installed at the bottom of the fixed plate 44. The top of the fixed plate 44 is fixedly installed with a first motor 47. A first gear 48 is fixedly installed on the output shaft of the first motor 47. A first toothed plate 49 is fixedly installed on the inner wall of one side of the mounting groove 43. The first gear 48 meshes with the first toothed plate 49.
[0018] When the first motor 47 is started, the first motor 47 drives the first gear 48 to rotate. While rotating, the first gear 48 moves on the first gear plate 49. The first gear 48 drives the fixing plate 44 to move in the mounting groove 43 through the first motor 47, thereby enabling the connector 10 to move in one horizontal direction.
[0019] The mounting groove 43 has sliding grooves 45 on both sides of its inner wall, and the fixing plate 44 has sliders 46 fixedly installed on both sides, and the sliders 46 are slidably installed in the sliding grooves 45.
[0020] The second moving mechanism 6 includes a rotating shaft 62 rotatably mounted on the top of the moving plate 41. A plurality of rotating seats 61 are fixedly mounted on the top of the moving plate 41. The rotating shaft 62 is rotatably mounted on the rotating seats 61. A second gear 67 is fixedly mounted on both ends of the rotating shaft 62. A fixing strip 66 is fixedly mounted on the top of the first guide strip 3 below the second gear 67. A second toothed plate 68 is fixedly mounted on the top of the fixing strip 66. The second gear 67 meshes with the second toothed plate 68. A second motor 63 is fixedly mounted on the top of the moving plate 41. The second motor 63 is connected to the rotating shaft 62.
[0021] A first bevel gear 64 is fixedly mounted on the output shaft of the second motor 63, and a second bevel gear 65 is fixedly sleeved on the outer side of the rotating shaft 62. The second bevel gear 65 meshes with the first bevel gear 64.
[0022] The connecting plate 9 is hollow, the connector 10 is connected to the connecting plate 9, and an air pump 17 is fixedly installed on the top of the connecting plate 9. The air inlet of the air pump 17 is connected to the connecting plate 9.
[0023] Multiple electromagnets 18 are fixedly installed at the bottom of the connecting plate 9. The electromagnets 18 are sleeved on the outside of the connector 10. The bottom of the electromagnet 18 is provided with an iron ring 19, which is sleeved on the outside of the connector 10. A rubber sleeve is provided on the outside of the iron ring 19.
[0024] The second motor 63 is started, which drives the first bevel gear 64 to rotate. The first bevel gear 64 drives the second bevel gear 65 to rotate. The second bevel gear 65 drives the rotating shaft 62 to rotate. The rotating shaft 62 drives the second gear 67 to rotate, causing the second gear 67 to move on the second gear plate 68. The second gear 67 drives the moving plate 41 to move through the rotating shaft 62 and the rotating seat 61, thereby realizing the movement of the connector 10 in another horizontal direction.
[0025] The working principle of the 96-well enzyme-labeled plate washing device provided by this invention is as follows: When cleaning a 96-well microplate is required, first, install the 96-well microplate on the microplate stand 15. Then, move the hydraulic cylinder 8, connecting plate 9, and connector 10 using the first moving mechanism 4 and the second moving mechanism 6, moving connector 10 to directly above the clean pipette tip box 13. Next, move the connecting plate 9 and connector 10 downwards using the hydraulic cylinder 8, inserting connector 10 into the pipette tip 5 inside the clean pipette tip box 13. Then, reverse the hydraulic cylinder 8 to remove the pipette tip 5 from the clean pipette tip box 13. Then, move connector 10 with the pipette tip 5 inserted to directly above the washing solution tank 12 using the first moving mechanism 4 and the second moving mechanism 6. Then, activate the hydraulic cylinder 8 to insert the pipette tip 5 into the washing solution tank 12. Finally, activate the air pump 17 to draw the cleaning solution from the washing solution tank 12 into the pipette tip 5. The first moving mechanism 4, the second moving mechanism 6, and the hydraulic cylinder 8 move the pipette tip 5, which has been sucking up the cleaning solution, to the top of the ELISA plate platform 15. Then, the air pump 17 pumps the cleaning solution into the 96-well ELISA plate on the ELISA plate platform 15 that needs to be cleaned. Then, the shaking device 14 is started to shake the 96-well ELISA plate to mix it. After that, the solution is manually poured out and patted dry. Then, the above operation is repeated several times until the plate is cleaned. The first moving mechanism 4, the second moving mechanism 6, and the hydraulic cylinder 8 are started to move the used pipette tip to the top of the waste pipette tip container 16. Then, the electromagnet 18 is de-energized, and the electromagnet 18 loses its magnetism. Under the action of gravity, the iron ring 19 strikes the used pipette tip 5, thereby removing the used pipette tip 5 from the connector 10, thus completing the collection of waste pipette tips and the cleaning of the 96-well ELISA plate.
[0026] Compared with related technologies, the 96-well enzyme-labeled plate washing device provided by the present invention has the following advantages: This invention provides a 96-well enzyme-labeled plate washing device. Through a first moving mechanism 4 consisting of a moving plate 41, a moving block 42, a mounting groove 43, a fixed plate 44, a sliding groove 45, a slider 46, a first motor 47, a first gear 48, and a first toothed plate 49, and a second moving mechanism 6 consisting of a rotating seat 61, a rotating shaft 62, a second motor 63, a first bevel gear 64, a second bevel gear 65, a fixed strip 66, a second gear 67, and a second toothed plate 68, the connector 10 can move at any horizontal position. Simultaneously, in conjunction with a hydraulic cylinder 8, the connector 10 can move between the clean pipette tip box 13, the enzyme-labeled plate platform 15, and the waste pipette tip container 16. With the air pump 17, the pipette tip 5 can draw the washing solution through the connecting plate 9 and the connector 10. With the electromagnet 18 and the iron ring 19, the used pipette tip 5 can be quickly removed from the connector 10, thereby enabling the collection of waste pipette tips 5.
[0027] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.
Claims
1. A 96-well enzyme-labeled plate washing device, characterized in that, include: The base plate has two side plates symmetrically fixedly installed on its top. A first guide strip is fixedly installed on the side of the two side plates that are close to each other. A first moving mechanism is installed between the two first guide strips. A hydraulic cylinder is installed on the first moving mechanism. A connecting plate is fixedly installed on the bottom of the hydraulic cylinder. A plurality of rectangularly distributed connectors are fixedly installed on the bottom of the connecting plate. A second moving mechanism is provided on the first moving mechanism. The second moving mechanism is connected to the two first guide strips. The base plate is provided with a mounting plate on top, and the mounting plate is provided with a washing solution tank, at least one clean pipette tip box, a shaking device, and an exhaust pipette tip bucket on top. The shaking device is provided with an enzyme-labeled plate platform.
2. The 96-well enzyme-labeled plate washing device according to claim 1, characterized in that, The first moving mechanism includes a moving plate, on both sides of which a moving block is fixedly installed. The moving block is slidably installed in the corresponding first guide strip. The bottom of the moving plate has an installation groove, in which a fixed plate is slidably installed. The hydraulic cylinder is fixedly installed at the bottom of the fixed plate. The top of the fixed plate has a first motor fixedly installed. The output shaft of the first motor has a first gear fixedly installed. A first toothed plate is fixedly installed on the inner wall of one side of the installation groove. The first gear meshes with the first toothed plate.
3. The 96-well enzyme-labeled plate washing device according to claim 2, characterized in that, The mounting groove has sliding grooves on both sides of its inner wall, and sliders are fixedly installed on both sides of the fixing plate, with the sliders slidably installed in the sliding grooves.
4. The 96-well enzyme-labeled plate washing device according to claim 2, characterized in that, The second moving mechanism includes a rotating shaft rotatably mounted on the top of the moving plate. Second gears are fixedly mounted on both ends of the rotating shaft. A fixing strip is fixedly mounted on the top of the first guide strip below the second gear. A second toothed plate is fixedly mounted on the top of the fixing strip. The second gear meshes with the second toothed plate. A second motor is fixedly mounted on the top of the moving plate. The second motor is connected to the rotating shaft.
5. The 96-well enzyme-labeled plate washing device according to claim 4, characterized in that, A first bevel gear is fixedly mounted on the output shaft of the second motor, and a second bevel gear is fixedly sleeved on the outer side of the rotating shaft. The second bevel gear meshes with the first bevel gear.
6. The 96-well enzyme-labeled plate washing device according to claim 1, characterized in that, The connecting plate is hollow, the connector is connected to the connecting plate, and an air pump is fixedly installed on the top of the connecting plate, with the air inlet of the air pump connected to the connecting plate.
7. The 96-well enzyme-labeled plate washing device according to claim 6, characterized in that, Multiple electromagnets are fixedly installed at the bottom of the connecting plate. The electromagnets are sleeved on the outside of the connector. The bottom of the electromagnet is provided with an iron ring, which is also sleeved on the outside of the connector.