A device and method for purifying siliceous sponge spicules

Abstract

The application discloses a purification device and method for siliceous sponge spicules, which adopts a purification method based on strain preparation, and has the advantages of high recovery rate of uniaxial siliceous sponge spicules, low broken needle rate, high purity and low breakage. The gravity separation technology is adopted to realize large-scale industrial production. The freeze-drying device is provided with a plurality of swing shafts symmetrically arranged on the barrel cover, one side of each swing shaft is rotationally connected with the barrel cover, the other side of each swing shaft is rotationally arranged on a supporting plate, a plurality of supporting rods are fixedly arranged on the swing shafts, the supporting rods are used for supporting sponge plates, a striking table is fixed on the barrel cover to support part of the supporting rods, a swing driving assembly is arranged on one side of each swing shaft, the swing driving assembly can drive the swing shaft to rotate by a certain angle and then automatically fall off, after the supporting rods fall off with the swing shaft, the supporting rods strike the striking table, so that the sponge plates on the supporting rods vibrate, when freeze-drying is performed, the vibrating sponge plates can make the sponge turn over to a certain extent, the freeze-drying effect is greatly improved, and the freeze-drying quality of the sponge is more uniform.

Description

TECHNICAL FIELD

[0001] The present application relates to sponge spicules, in particular to a purification device and method of siliceous sponge spicules. BACKGROUND

[0002] Modern scientific research has found that sponges contain a special skeletal structure, which is mainly composed of silicon dioxide or calcium carbonate. The siliceous spicule is catalyzed by silicate protease in the body of siliceous sponge animals, and has various structures, such as uniaxial spicule, four-spoke spicule, three-spoke spicule, and a kind of gemma small spicule.

[0003] Through the study of the mineralization mechanism and biomimetic application of sponge spicules, it is found that the special microcosmic concentric structure and biological silicon composition make it have hard and strong toughness, and have broad application prospect. In recent years, the application of sponge spicules in the field of biomedical materials is increasing, and the demand for them is increasing exponentially, and the price of raw materials is also rising.

[0004] At present, the traditional purification methods of sponge spicules are mostly alkali treatment method, oxidation method, physical separation method and enzymatic hydrolysis method. The alkali treatment method is simple in operation, low in cost, suitable for large-scale production, can effectively remove most of the organic impurities, and has high purity, but strong alkali may damage the surface structure of the microneedle, affect its mechanical properties, and need to strictly control the pH and temperature (usually 60-80℃), otherwise the microneedle may be dissolved, and the residual alkali needs to be washed several times, which may increase the environmental protection cost; the oxidation method has little damage to siliceous microneedles, is suitable for preserving the nano-scale surface pore structure, and hydrogen peroxide can sterilize at the same time, reducing the subsequent processing steps, but the oxidation is not complete, and alkali treatment or ultrasonic assistance is often needed, and NaClO may introduce chlorine residues, affecting product safety; the physical separation method has no chemical reagents, is suitable for products with not extremely high purity requirements, can preserve the natural surface characteristics of microneedles (such as porous structure), but the purity is relatively low (60-80%), and multiple separation is needed, which has high requirements for the uniformity of microneedle size, otherwise the efficiency is low; the enzymatic hydrolysis method has mild conditions (pH 7-8, 37-50℃), preserves the integrity of the microneedle structure, has high specificity, avoids chemical residues, and is suitable for medical-grade high-purity requirements, but the enzyme cost is high, the reaction time is long (24-72 hours are needed), multiple enzymes (such as protease + lipase) need to be used to completely remove impurities, the process is complex, and enzyme proteins may be left, which need to be inactivated.

[0005] Therefore, on the basis of not damaging the morphology of sponge spicules, it is an urgent problem for researchers to provide a technology that is easy to realize industrial production, and can greatly improve the recovery rate and purity of spicules. SUMMARY

[0006] The purpose of the present application is to provide a purification device and method of siliceous sponge spicules to solve the above problems.

[0007] The above technical objective of the present application is achieved by the following technical solution: a purification method of siliceous sponge spicule, comprising the following steps:

[0008] Step 1: soak the untreated sponge raw material with clean water, stir and precipitate, and then filter out the silt; after centrifugal dewatering, freeze-drying equipment is used for freeze-drying, and then the sponge body powder is obtained by crushing.

[0009] Step 2: high-pressure steam sterilization is performed on the obtained sponge body powder to obtain sponge fragments.

[0010] Step 3: inoculate the working strain Streptomyces XL-34 into Gause No. 1 culture medium, and culture at 30℃ and 150rpm for 24 hours until OD600=1.0; mix the sponge fragments and the bacterial suspension in a sterile container at a solid-liquid ratio of 1:10-20mL, and shake well.

[0011] Step 4: set the fermentation temperature to 30℃, adjust the pH value to 7.2, and aerate the container containing the sponge fragments and the working strain for 3-7 days; when the organic matter is below the threshold value, terminate the fermentation.

[0012] Step 5: heat at 80℃ for 20min to terminate the activity of the bacterial population, and use ultrasonic cleaning to remove residual bacterial bodies and decomposition products.

[0013] Step 6: remove trace organic matter while retaining the inorganic framework by calcining at 300℃ for 10min.

[0014] Step 7: perform liquid-phase gravity separation on the product treated in step 6, and obtain single-axis siliceous sponge spicules after drying.

[0015] The freeze-drying equipment used in the above-mentioned purification method of siliceous sponge spicule comprises a base, a support fixedly installed on the base, and a freeze-drying barrel fixedly installed on the support; a first gas pipe and a second gas pipe are fixedly connected to the freeze-drying barrel; the first gas pipe is used for vacuum pumping and is connected to an external vacuum pumping system; the second gas pipe is used for discharging water vapor formed by ice sublimation and is connected to an external vapor condensation and collection system; and an evaporator is further installed in the bottom of the base.

[0016] A barrel cover is arranged on one side of the freeze-drying barrel; the barrel cover is used for closing the freeze-drying barrel; a locking structure is arranged between the barrel cover and the freeze-drying barrel; a moving seat is fixedly installed at the bottom of the barrel cover; and a roller is installed at the bottom of the moving seat.

[0017] A plurality of swing shafts are symmetrically arranged on the barrel cover, one side of the swing shaft is rotatably connected with the barrel cover, the other side of the swing shaft is rotatably arranged on a supporting piece, a plurality of supporting rods are fixedly arranged on the swing shaft, the supporting rods are used for supporting the sponge disc, an impact platform is fixed on the barrel cover to support part of the supporting rods, a swing driving assembly is arranged on one side of the swing shaft, the swing driving assembly can drive the swing shaft to rotate by a certain angle and then fall by itself, the supporting rods hit the impact platform after falling with the swing shaft, so that the sponge discs on the supporting rods vibrate, and the vibrating sponge discs can make the sponge turn over.

[0018] Preferably, a vibration head is arranged through the impact platform, springs are fixed between the vibration head and the impact platform, the vibration head is slidably connected with the impact platform, and one end of the vibration head abuts against the supporting rod.

[0019] Preferably, a collecting box is fixedly installed at the center position of the barrel cover, the supporting piece is fixed with the collecting box, the swing shafts are symmetrically arranged around the collecting box, a collecting chamber is formed in the collecting box, a plurality of collecting openings communicating with the collecting chamber are symmetrically formed in the collecting box, guide plates fixed with the collecting box are arranged at the bottom of the collecting openings, each guide plate is arranged at the bottom of one swing shaft, and a discharge nozzle is arranged at the bottom of the collecting box.

[0020] Preferably, the swing driving assembly comprises a rotating shaft fixed on one side of each swing shaft, a gear is fixed at one end of the rotating shaft, each gear is meshed with a rack, a matching plate is fixedly arranged between the two racks, the matching plate is slidably arranged on the collecting box, a cam is arranged on one side of the matching plate, the cam is fixed on a power shaft, the power shaft penetrates the freeze-drying barrel and is fixed with a motor shaft of a motor, the motor is fixed on a motor base, and the motor base is fixed with the base.

[0021] Preferably, at least two guide rods are fixed on the matching plate, each guide rod penetrates a guide seat, and the guide seat is fixedly arranged on the collecting box.

[0022] Preferably, two slide rails are symmetrically fixed on the base, slide blocks are slidably installed in the slide rails, a connecting strip is fixedly connected with one side of the slide blocks, and the connecting strip is fixed with the moving base.

[0023] Preferably, an electric control integrated box is fixedly arranged on the base.

[0024] In summary, the present application has the following beneficial effects:

[0025] 1. The single-axis siliceous sponge spicule prepared by the purification method based on the strain has high recovery rate, low broken needle rate, high purity and is not easy to break.

[0026] 2. The gravity separation technology is adopted, and large-scale industrial production can be realized.

[0027] 3. The freeze-drying equipment is provided with a plurality of swing shafts symmetrically arranged on the barrel cover, one side of the swing shaft is rotationally connected with the barrel cover, the other side of the swing shaft is rotationally arranged on the supporting plate, a plurality of supporting rods are fixedly arranged on the swing shaft, the supporting rods are used for supporting the sponge disc, a striking table is fixed on the barrel cover to support part of the supporting rods, a swing driving assembly is arranged on one side of the swing shaft, the swing driving assembly can drive the swing shaft to rotate by a certain angle and then automatically fall off, the supporting rods fall off with the swing shaft and then strike the striking table, so that the sponge discs on the supporting rods all vibrate, and thus when freeze-drying is performed, the vibrating sponge discs can make the sponge turn over to a certain extent, the freeze-drying effect is greatly improved, and the freeze-drying quality of the sponge is more uniform.

[0028] 4. A vibrating head is arranged through the striking table, springs are fixed between the vibrating head and the striking table, the vibrating head and the striking table are in sliding connection, one end of the vibrating head abuts against the supporting rod, and the arrangement of the vibrating head and the springs makes the supporting rod directly strike the vibrating head after falling off, the vibrating head will descend and stretch the springs, so as to slow down the striking of the supporting rod and the striking table, and different elastic coefficients of the springs can bring different striking effects, so as to change the vibrating effect of the sponge disc on the supporting rod and adapt to sponge particles of different sizes.

[0029] 5. The swing driving assembly comprises a rotating shaft fixed on one side of each swing shaft, a gear is fixed on one end of the rotating shaft, each gear is in meshing connection with a rack, a matching plate is fixedly arranged between the two racks, the matching plate is slidingly arranged on the collecting box, a cam is arranged on one side of the matching plate, the cam is fixed on a power shaft, the power shaft penetrates through the freeze-drying barrel and is fixed with a motor shaft of a motor, the motor is fixed on a motor base, and the motor base is fixed with the base.

[0030] The matching plate is manually pushed downward to drive the rotating shaft to rotate by a certain angle, the sponge discs on the supporting rods will be inclined, and the sponge particles in the sponge discs will enter the collecting port along the guide plate under the action of their own gravity and finally enter the collecting chamber, the sponge particles in the collecting chamber will be discharged from the discharge nozzle, and the user only needs to place a storage container at the bottom of the discharge nozzle to collect all the freeze-dried sponge particles in the sponge discs at one time, which is very convenient. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description only show some embodiments of the application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0032] Figure 1 is a schematic view of the appearance of the purification equipment;

[0033] Figure 2 is a schematic diagram of the internal structure of the purification device;

[0034] Figure 3 is Figure 2 is an enlarged schematic diagram of A in

[0035] Figure 4 is Figure 2 is a sectional view of

[0036] Figure 5 is a side view of Figure 2

[0037] Figure 6 is an enlarged schematic diagram of B in Figure 5

[0038] Figure 7 is a sectional view of Figure 1

[0039] In the figure: 11, base; 12, moving seat; 14, roller; 15, barrel cover; 16, slide rail; 17, connecting strip; 18, sliding block; 19, electric control integrated box; 20, motor base; 21, motor; 22, power shaft; 23, freeze-drying barrel; 24, No. 1 air pipe; 25, No. 2 air pipe; 26, collection box; 27, collection port; 28, swing shaft; 29, support rod; 30, impact platform; 31, guide plate; 32, collection chamber; 33, discharge nozzle; 34, matching plate; 35, support piece; 36, rotating shaft; 37, gear; 38, rack; 39, guide rod; 40, guide seat; 41, vibration head; 42, spring; 43, cam; 44, locking structure. DETAILED DESCRIPTION

[0040] A method for purifying siliceous sponge spicules, comprising the following steps: EXAMPLE

[0041] Step 1: Soak the untreated sponge raw material with water for 1-4 times, stir and precipitate, filter out the silt, centrifugal dewatering, freeze-drying by freeze-drying equipment, then crush, further destroy the organic matrix structure, get sponge powder, the sponge powder increases the surface area, facilitates microbial penetration.

[0042] Step 2: Steam sterilization of the obtained sponge powder to obtain sponge fragments; steam temperature is set to 101-133℃, sterilization for 7-20 minutes.

[0043] Step 3: Inoculate the working strain Streptomyces XL-34 into Gause No. 1 medium, 30℃, 150rpm shaking culture for 24 hours to OD600=1.0, mix the sponge fragments and bacterial suspension in a sterile container according to the solid-liquid ratio of 1:10~20mL, and shake well. ​​​

[0044] Step 4: Set the fermentation temperature to 30℃, adjust the pH value to 7.2 with NaOH / HCl, aerate the container containing sponge fragments and working strains (0.5-1vvm), and ferment for 3-7 days (sample daily to monitor degradation rate), and terminate fermentation when the organic matter is below the threshold value (≤0.05%).

[0045] Step 5: Heat at high temperature 80℃ for 20min to terminate the activity of the bacterial population, and use ultrasonic cleaning to remove residual bacterial bodies and decomposition products.

[0046] Step 6: Remove trace organic matter by calcining at high temperature 300℃ for 10min while retaining the inorganic skeleton.

[0047] Step 7: Perform liquid gravity separation on the product treated in step 6, adjust the inclination of the separation bed surface and the flow rate of the flushing water flow to allow impurities and spicules to naturally distribute due to gravity and particle size differences, collect the materials in different areas, and dry to obtain high-purity products.

[0048] The purified uniaxial siliceous sponge spicule obtained has a purity of ≥99%, a broken needle rate of less than 10%, and a recovery rate of 80%. Example

[0049] This example provides a purification method for siliceous sponge spicules according to the present application. Compared with Example 1:

[0050] Steps 1 and 2 are consistent;

[0051] In step 3, two strains of Streptomyces XL-34 and Bacillus subtilis are inoculated, Streptomyces XL-34 is inoculated into Gause No. 1 medium, and cultured at 30℃ with 150rpm shaking for 24 hours until OD600≈1.0, Bacillus subtilis is inoculated into LB medium, and cultured at 30℃ with 150rpm shaking for 24 hours until OD600≈1.0; the inoculation ratio of the two strains is 2:1; digestion is performed according to a solid-liquid ratio of sponge fragments to bacterial solution of 1:20mL, and other conditions are consistent;

[0052] In step 4, the fermentation temperature is set to 32℃, the pH value is adjusted to 7.0 with NaOH / HCl, and other conditions are consistent;

[0053] In step 5, the bacterial population activity is terminated by heating at high temperature 90℃ for 10min;

[0054] Steps 6 and 7 are consistent.

[0055] In this example, the purified siliceous sponge spicule obtained has a purity of ≥99%, a broken needle rate of <15%, and a recovery rate of 87%.

[0056] Reference Figures 1-7The freeze-drying equipment used in the method for purifying siliceous sponge spicules comprises a base 11, a support fixedly installed on the base 11, a freeze-drying barrel 23 fixedly arranged on the support, a first air pipe 24 and a second air pipe 25 fixedly and communicatively arranged on the freeze-drying barrel 23, the first air pipe 24 being used for vacuumizing and being connected with an external vacuumizing system, the second air pipe 25 being used for discharging water vapor formed by ice sublimation and being connected with an external steam condensing and collecting system, and an evaporator (not shown, being a prior art and not shown in the drawing for the purpose of displaying the drawing and being consistent with a conventional freeze-drying equipment) further installed at the bottom of the base 11.

[0057] A barrel cover 15 is arranged on one side of the freeze-drying barrel 23, the barrel cover 15 being used for closing the freeze-drying barrel 23, a locking structure 44 being arranged between the barrel cover 15 and the freeze-drying barrel 23, the locking and unlocking of the barrel cover 15 and the freeze-drying barrel 23 being realized through the locking structure 44 (the locking structure can be used according to actual requirements, and any conventional locking structure in the art can be adopted in the present application), a moving seat 12 being fixedly arranged at the bottom of the barrel cover 15, and a roller 14 being installed at the bottom of the moving seat 12, the barrel cover 15 and the freeze-drying barrel 23 being designed in a pull-out type after being unlocked, so as to facilitate the taking and placing of sponge plates.

[0058] A plurality of swing shafts 28 are symmetrically arranged on the barrel cover 15, one side of each swing shaft 28 being rotatably connected with the barrel cover 15, the other side of each swing shaft 28 being rotatably arranged on a support plate 35, a plurality of supporting rods 29 being fixedly arranged on the swing shafts 28, the supporting rods 29 being used for supporting sponge plates, a striking table 30 being fixedly arranged on the barrel cover 15 to support part of the supporting rods 29, a swing driving assembly being arranged on one side of each swing shaft 28, the swing driving assembly being capable of driving the swing shaft 28 to rotate by a certain angle and then falling by itself, the supporting rods 29 falling along with the swing shaft 28 and then striking the striking table 30, so that the sponge plates on the supporting rods 29 are vibrated, and thus the vibrating sponge plates can make the sponge turn over to a certain extent during freeze-drying, greatly improving the freeze-drying effect and making the sponge freeze-drying more uniformly.

[0059] In particular, a vibrating head 41 is arranged through the striking table 30, a spring 42 being fixedly arranged between the vibrating head 41 and the striking table 30, the vibrating head 41 being slidably connected with the striking table 30, one end of the vibrating head 41 abutting against the supporting rod 29, the arrangement of the vibrating head 41 and the spring 42 enabling the vibrating head 41 to directly strike the supporting rod 29 after the supporting rod 29 falls, the vibrating head 41 being lowered and stretching the spring 42, so as to slow down the striking of the supporting rod 29 and the striking table 30, and different striking effects can be brought by replacing the spring 42 with different elastic coefficients, so as to change the vibrating effect of the sponge plates on the supporting rods 29 and adapt to sponge particles of different sizes.

[0060] A collecting box 26 is fixedly installed at the center of the bucket cover 15, the support plates 35 are fixed with the collecting box 26, the swing shafts 28 are symmetrically arranged with the collecting box 26 as the center, a collecting chamber 32 is formed in the collecting box 26, a plurality of collecting openings 27 are symmetrically formed on the collecting box 26 and communicate with the collecting chamber 32, guide plates 31 are arranged at the bottom of the collecting openings 27 and are fixed with the collecting box 26, each of the guide plates 31 is arranged at the bottom of one of the swing shafts 28, and a discharge nozzle 33 is arranged at the bottom of the collecting box 26;

[0061] The swing driving assembly comprises a rotating shaft 36 fixed at one side of each of the swing shafts 28, a gear 37 fixed at one end of the rotating shaft 36, each of the gears 37 engaged with a rack 38, a matching plate 34 fixedly arranged between the two racks 38, the matching plate 34 slidingly arranged on the collecting box 26, a cam 43 arranged at one side of the matching plate 34, the cam 43 fixed with the power shaft 22, the power shaft 22 penetrating the freeze-drying barrel 23 and fixed with the motor shaft of the motor 21, the motor 21 fixed with the motor base 20, the motor base 20 fixed with the base 11; the motor 21 drives the power shaft 22 to rotate, the power shaft 22 drives the cam 43 to rotate, the cam 43 drives the matching plate 34 to descend when the cam 43 rotates, until the cam 43 is separated from the matching plate 34, the matching plate 34 drives the rack 38 to descend, the rotating shaft 36 and the swing shaft 28 are driven to rotate through the engagement between the rack 38 and the gear 37, the swing shaft 28 drives the support rod 29 and the sponge disc on the support rod 29 to rotate, after the cam 43 is separated from the matching plate 34, the sponge disc on the support rod 29 falls under the action of its own gravity, drives the support rod 29 and the swing shaft 28 to rotate, the support rod 29 collides with the impact platform 30, drives the sponge particles in the sponge disc to be stirred;

[0062] At least two guide rods 39 are fixed on the matching plate 34, each of the guide rods 39 penetrates a guide seat 40, and the guide seat 40 is fixedly arranged on the collecting box 26.

[0063] Two slide rails 16 are symmetrically fixed on the base 11, a sliding block 18 is slidably installed in the slide rail 16, one side of the sliding block 18 is fixedly connected with a connecting strip 17, the connecting strip 17 is fixed with the moving seat 12, after the barrel cover 15 is manually pulled out, the matching plate 34 is manually pushed downward, the rotating shaft 36 is driven to rotate by a certain angle, the sponge disc on the supporting rod 29 is inclined, the sponge particles in the sponge disc are driven to enter the collecting port 27 along the guide plate 31 under the action of gravity, and finally enter the collecting chamber 32, the sponge particles in the collecting chamber 32 are discharged from the discharge nozzle 33, the user only needs to place a storage container at the bottom of the discharge nozzle 33 to collect all the freeze-dried sponge particles in the sponge disc at one time, which is very convenient; after the collection is completed, the empty sponge disc is manually taken away.

[0064] An electric control integrated box 19 is fixed on the base 11 to control the work of various electric appliances and provide power supply.

[0065] Freeze-drying equipment working principle:

[0066] The barrel cover 15 is manually pulled out, the sponge disc containing wet sponge particles is placed on the supporting rod 29, the barrel cover 15 is manually reset and locked through the locking structure 44, and the freeze-drying operation is performed after the locking is completed.

[0067] During the freeze-drying process, the motor 21 is controlled to start through the electric control integrated box 19, the motor 21 drives the power shaft 22 to rotate, the power shaft 22 drives the cam 43 to rotate, the cam 43 drives the matching plate 34 to descend when rotating, until the cam 43 is separated from the matching plate 34, the matching plate 34 drives the rack 38 to descend when descending, the rotating shaft 36 and the swing shaft 28 are driven to rotate through the meshing of the rack 38 and the gear 37, the supporting rod 29 and the sponge disc on the supporting rod 29 are driven to rotate by the swing shaft 28, after the cam 43 is separated from the matching plate 34, the sponge disc on the supporting rod 29 falls under the action of gravity, driving the supporting rod 29 and the swing shaft 28 to rotate, the supporting rod 29 collides with the impact table 30, driving the sponge particles in the sponge disc to tumble;

[0068] After the freeze-drying is completed, the motor 21 is turned off, the locking structure 44 is manually unlocked, the barrel cover 15 is pulled out, the matching plate 34 is manually pushed down, the rotating shaft 36 is rotated by a certain angle, the sponge disc on the supporting rod 29 is tilted, the sponge particles in the sponge disc are under the action of gravity, enter the collecting port 27 along the guide plate 31, and finally enter the collecting chamber 32, the sponge particles in the collecting chamber 32 are discharged from the discharge nozzle 33, and the user only needs to place a receiving container at the bottom of the discharge nozzle 33 to collect all the freeze-dried sponge particles in the sponge disc at one time.

[0069] The above embodiments are only for illustrating the technical concept and characteristics of the present application, and the purpose is to enable those skilled in the art to understand the content of the present application and implement it, and cannot limit the protection scope of the present application. Any equivalent changes or modifications made according to the spirit and essence of the present application shall be covered within the protection scope of the present application.

Claims

1. A freeze-drying device for purifying silica sponge bone needles, comprising a base (11), a bracket fixedly mounted on the base (11), a freeze-drying barrel (23) fixedly mounted on the bracket, a first gas pipe (24) and a second gas pipe (25) fixedly connected on the freeze-drying barrel (23), the first gas pipe (24) being used for vacuuming and connected to an external vacuuming system, the second gas pipe (25) being used for discharging and collecting water vapor formed by ice sublimation and connected to an external vapor condensation and collection system, and an evaporator also being installed at the bottom of the base (11), characterized in that: A lid (15) is provided on one side of the freeze-drying barrel (23). The lid (15) is used to close the freeze-drying barrel (23). A locking structure (44) is provided between the lid (15) and the freeze-drying barrel (23). A movable seat (12) is fixed at the bottom of the lid (15). A roller (14) is installed at the bottom of the movable seat (12). A plurality of swing shafts (28) are symmetrically arranged on the bucket lid (15). One side of the swing shaft (28) is rotatably connected to the bucket lid (15), and the other side of the swing shaft (28) is rotatably arranged on the support plate (35). A plurality of support rods (29) are fixedly arranged on the swing shaft (28). The support rods (29) are used to support the sponge disc. An impact platform (30) is fixed on the bucket lid (15) to support part of the support rods (29). A swing drive assembly is arranged on one side of the swing shaft (28). The swing drive assembly includes a rotating shaft (36) fixed to one side of each swing shaft (28), a gear (37) fixed at one end of the rotating shaft (36), each gear (37) meshing with a rack (38), a mating plate (34) fixed between the two racks (38), the mating plate (34) being slidably disposed on the collection box (26), a cam (43) being disposed on one side of the mating plate (34), the cam (43) being fixed on the power shaft (22), the power shaft (22) passing through the freeze-drying barrel (23) and being fixed to the motor shaft of the motor (21), the motor (21) being fixed on the motor base (20), and the motor base (20) being fixed to the base (11); A vibrating head (41) is provided through the impact platform (30), and a spring (42) is fixed between the vibrating head (41) and the impact platform (30). The vibrating head (41) is slidably connected to the impact platform (30), and one end of the vibrating head (41) abuts against the support rod (29). The swing drive assembly can drive the swing shaft (28) to rotate and then fall down on its own. After the support rod (29) falls down with the swing shaft (28), it hits the impact platform (30), causing the sponge discs on the support rod (29) to vibrate. The vibrating sponge discs will cause the sponge to flip.

2. The freeze-drying equipment according to claim 1, characterized in that: A collection box (26) is fixedly installed at the center of the bucket lid (15). The support plate (35) is fixed to the collection box (26). The swing shaft (28) is symmetrically arranged with the collection box (26) as the center. A collection chamber (32) is opened inside the collection box (26). Several collection ports (27) communicating with the collection chamber (32) are symmetrically opened on the collection box (26). A guide plate (31) fixed to the collection box (26) is provided at the bottom of the collection port (27). Each guide plate (31) is mounted on the bottom of one of the swing shafts (28). A discharge nozzle (33) is provided at the bottom of the collection box (26).

3. The freeze-drying equipment according to claim 1, characterized in that: At least two guide rods (39) are fixed on the mating plate (34), each of the guide rods (39) passing through the guide seat (40), the guide seat (40) being fixedly mounted on the collection box (26).

4. The freeze-drying equipment according to claim 1, characterized in that: Two slide rails (16) are symmetrically fixed on the base (11). A slider (18) is slidably installed in the slide rail (16). A connecting strip (17) is fixed on one side of the slider (18). The connecting strip (17) is fixed to the movable seat (12).

5. The freeze-drying equipment according to claim 1, characterized in that: An integrated electrical control box (19) is fixedly installed on the base (11).

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