Combined regulator for improving sensory quality of tobacco leaves and application thereof

Abstract

The invention provides a combined regulator for improving sensory quality of tobacco leaves and application of the combined regulator. The combined regulator is prepared from the following components in parts by weight: 30 to 60 parts of mineral source potassium fulvate, 30 to 60 parts of gamma-polyglutamic acid, 30 to 60 parts of deep-sea fish protein fertilizer and 0.9 to 1.8 parts of phosphoenolpyruvic acid. The combined regulator provided by the invention is low in raw material price, and can improve the fertilizer utilization rate of crops, promote the growth of flue-cured tobacco roots, improve the soil structure, improve the sensory quality of tobacco leaves and improve the aroma of the tobacco leaves.

Description

Technical Field

[0001] This invention belongs to the field of tobacco planting technology, specifically relating to a combination regulator for improving the sensory quality of tobacco leaves and its application. Background Technology

[0002] Flue-cured tobacco is one of the world's most important economic crops, and the tobacco industry is a major pillar of the global economy. However, the long-term and excessive application of chemical fertilizers not only increases nutrient residues in the soil, leading to soil and water pollution and reduced fertilizer utilization, but also may negatively impact tobacco yield and quality. Therefore, improving fertilizer utilization and the soil microenvironment are crucial for promoting the green development of the tobacco economy and the tobacco industry.

[0003] CN108456065A discloses an organic compound fertilizer for tobacco cultivation and its preparation method, comprising 5-20 parts by weight of ammonium hydrogen phosphate, 3-6 parts by weight of potassium dihydrogen phosphate, 7-12 parts by weight of mushroom substrate, 2-6 parts by weight of boric acid, 2-6 parts by weight of polyvinylpyrrolidone, 2-3 parts by weight of dimethyl tetrachloroethylene, 3-6 parts by weight of humic acid, 0.5-2 parts by weight of ascorbic acid, 3-6 parts by weight of ammonium sulfate, and 8-12 parts by weight of wood ash. This invention improves the absorption effect of tobacco leaves by combining organic and inorganic fertilizers, ensuring normal growth of tobacco leaves. In particular, by simultaneously fermenting trace element additives with wood ash and rabbit manure, the trace elements can be evenly integrated with the fermentation products, which is more conducive to the absorption of trace elements by tobacco plants. Compared with existing organic compound fertilizers, this method is more suitable for tobacco cultivation.

[0004] CN110590455A discloses a special fertilizer for tobacco cultivation and its application method. The fertilizer uses fermented rapeseed meal as the organic component and ammonium nitrate, potassium nitrate, potassium sulfate, diammonium phosphate, borax, ammonium molybdate, and magnesium sulfate as the inorganic component to produce an organic-inorganic compound fertilizer. The resulting fertilizer has a formula of N-P₂O₅-K₂O = 10-16-12, is granular, and contains ≥0.5% B, ≥0.1% Mo, ≥5% MgO, and ≥35% nitrate nitrogen. This invention increases the phosphorus content in the tobacco fertilizer, which is beneficial for phosphate absorption; it effectively reduces nutrient loss and prolongs fertilizer effectiveness; the nutrient ratio is more reasonable, promoting the quality and efficiency of tobacco and preventing nutrient deficiency. Compared with conventionally used powdered organic-inorganic compound fertilizers, it is more beneficial for tobacco growth in the early stages of tobacco cultivation in Changning, Hunan Province, under conditions of high temperature and heavy rainfall.

[0005] CN103058760A discloses a slow-release compound fertilizer suitable for tobacco cultivation, belonging to a mixture of various fertilizers containing substances affecting urea nitrification in soil and components without special fertilizer effects. It is made from the following raw materials in parts by weight: urea 20-45%, ammonium sulfate 10-20%, potassium sulfate 20-40%, mixed phosphate fertilizer 10-30%, zinc, copper, boron, and molybdenum additives 0.1-0.5%, granulation aid 6-12%, and appropriate amount of water. The preparation method includes the following steps: ① preparation of potassium and nitrogen fertilizer complex, ② preparation of mixed phosphate fertilizer, ③ preparation of mixed granules of each component of the compound fertilizer, ④ stearic acid coating, ⑤ powdering with modified zeolite powder material, and ⑥ sieving. This invention overcomes traditional biases, reduces production costs, and is beneficial to environmental protection. It provides a slow-release compound fertilizer suitable for tobacco cultivation that is nutritionally comprehensive, has excellent slow-release performance, fully meets the nutrient requirements of tobacco, has high fertilizer utilization rate, and can improve both tobacco yield and quality.

[0006] The excessive use of chemical fertilizers currently leads to increased nutrient residues in the soil, ultimately impacting tobacco yield and quality. Therefore, finding a method to improve tobacco quality from the source has become an urgent problem to solve. Summary of the Invention

[0007] To address the shortcomings of existing technologies, the present invention aims to provide a combined regulator for improving the sensory quality of tobacco leaves and its application. The combined regulator provided by the present invention uses inexpensive raw materials, can improve fertilizer utilization in crops, promote root growth in flue-cured tobacco, improve soil structure, enhance the sensory quality of tobacco leaves, and increase the aroma of tobacco leaves.

[0008] To achieve this objective, the present invention adopts the following technical solution:

[0009] In a first aspect, the present invention provides a combined regulator for improving the sensory quality of tobacco leaves, wherein the combined regulator comprises, by weight, 30-60 parts of mineral-derived potassium humate, 30-60 parts of γ-polyglutamic acid, 30-60 parts of deep-sea fish protein fertilizer, and 0.9-1.8 parts of phosphoenol pyruvate.

[0010] The proportions of potassium humate can be 30, 35, 40, 45, 50, 55, or 60 parts, the proportions of γ-polyglutamic acid can be 30, 35, 40, 45, 50, 55, or 60 parts, the proportions of deep-sea fish protein fertilizer can be 30, 35, 40, 45, 50, 55, or 60 parts, and the proportions of phosphatenolpyruvic acid can be 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, or 1.8 parts, but are not limited to the values ​​listed above. Other unlisted values ​​within the above range are also applicable.

[0011] Among the above components, γ-polyglutamic acid can improve fertilizer utilization in flue-cured tobacco, promote root development and enhance stress resistance, improve soil structure and balance pH, thereby increasing the yield and quality of flue-cured tobacco. Potassium humate from mineral sources helps loosen the soil, retain water and prevent drought, and has nitrogen-fixing, phosphorus-solubilizing, potassium-activating, and micronutrient effects. Deep-sea fish protein is a high-quality protein extracted from deep-sea fish, rich in amino acids, including essential amino acids required for crop growth. These amino acids can be directly absorbed and utilized by crops, promoting their growth and development. In addition, deep-sea fish protein also contains abundant trace elements such as zinc, iron, and copper, which play an important role in crop growth and yield improvement. Phosphoenolpyruvate is a key substrate in the biosynthesis of phenylalanine, which can be synthesized through a series of reactions such as degradation, oxidation, and deamination to produce phenylethanol and phenylacetaldehyde, important aroma components of flue-cured tobacco. The combination of these four components works synergistically to effectively improve the sensory quality and aroma of tobacco leaves.

[0012] Preferably, the combined regulator comprises, by weight, 35-55 parts of mineral-derived potassium humate, 35-55 parts of γ-polyglutamic acid, 35-55 parts of deep-sea fish protein fertilizer, and 1.05-1.65 parts of phosphoenol pyruvate.

[0013] Secondly, the present invention provides the application of the combined regulators for improving the sensory quality of tobacco leaves as described above in tobacco cultivation.

[0014] Thirdly, the present invention provides a tobacco planting method in which the combined regulators for improving the sensory quality of tobacco leaves, as described above, are added during topdressing.

[0015] Preferably, the total amount of the combined regulator added is 91.5-183 kg / hm. 2 For example, 91.5 kg / hm 2 100kg / hm 2 110kg / hm 2 120kg / hm 2 130kg / hm 2 140kg / hm 2 150kg / hm 2 160kg / hm 2 170kg / hm 2 180kg / hm 2 Or 183 kg / hm 2 The values ​​listed above, but not limited to those listed above, also apply to other unlisted values ​​within the range mentioned above.

[0016] Preferably, the process of adding the combined regulator for improving the sensory quality of tobacco leaves as described above during fertilization specifically involves:

[0017] The combined regulator was mixed with water and applied as a hole irrigation during the seedling stage and the vigorous growth stage, respectively.

[0018] Preferably, the mass ratio of the combined regulator used during the crowning stage and the vigorous growth stage is (2.7-3.3):(1.7-2.3), wherein the number of parts of the combined regulator used during the crowning stage can be 2.7, 2.8, 2.9, 3, 3.1, 3.2 or 3.3, etc., and the number of parts of the combined regulator used during the vigorous growth stage can be 1.7, 1.8, 1.9, 2, 2.1, 2.2 or 2.3, etc., but is not limited to the values ​​listed above. Other values ​​not listed within the above range are also applicable.

[0019] Preferably, the mass ratio of the combined regulator to water is 1:(30-50), such as 1:30, 1:35, 1:40, 1:45 or 1:50, but not limited to the values ​​listed above. Other values ​​not listed within the above range are also applicable.

[0020] Preferably, the seedling stage is 18-24 days after transplanting.

[0021] Preferably, the vigorous growth period is 42-48 days after the tobacco seedlings are transplanted.

[0022] The aforementioned specific planting method can effectively improve the sensory quality of tobacco leaves by controlling the application of combined growth regulators.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] This invention provides a combined regulator to improve the sensory quality of tobacco leaves. By using a compound of four components that work synergistically and combined with specific planting methods, it can effectively improve the sensory quality and aroma of tobacco leaves. Detailed Implementation

[0025] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0026] In the following example, the mineral-derived potassium humate was purchased from Shandong Cangyuan Biotechnology Co., Ltd.

[0027] The deep-sea fish protein fertilizer was purchased from Weifang Aofeng Crop Disease Control Co., Ltd.

[0028] γ-polyglutamic acid was purchased from Shandong Hengke Biotechnology Co., Ltd.

[0029] The following examples each provide a tobacco cultivation method, and the general cultivation process is as follows:

[0030] In 2023, an experiment was conducted in Pangxie Village, Tianbaqiao Town, Qixingguan District, Bijie City, Guizhou Province, using Yunyan 87 as the test variety, covering an area of ​​200 mu. The specific practices of the demonstration group were as follows:

[0031] Seedlings were sown on February 9th using a floating seedling raising method. The seedling trays were filled with 2000 kg of water, and tobacco-specific fertilizer (purchased from Guizhou Ketai Jinfu Agricultural Development Co., Ltd.) was added at a ratio of 0.3% (mass fraction). Seedlings emerged on March 8th. By March 22nd, the seedlings had 4 leaves and 1 heart, and the leaves were pruned once. On April 3rd, the leaves were pruned once more. By April 16th, the seedlings had reached 5 leaves and 1 heart, with a height of 6-7 cm, green color, strong growth, robust and disease-free, and flexible stems. The seedlings were transplanted on April 19th. When transplanting, the roots of the seedlings were pulled out of the seedling trays along with the nutrient substrate.

[0032] On March 10th, fertilization and ridging were carried out. Rows were marked with a spacing of 1.2m, and furrows 18-24cm wide and 15-20cm deep were dug using these marked lines as the center line. Base fertilizer was applied according to local standards. Ridging was then carried out using a ridging machine, followed by manual trimming to achieve a ridge height of 27cm, a base width of 1.08m, and a top width of 57cm. Mulching was carried out on the same day the ridges were formed.

[0033] Transplanting was carried out on April 19th. Holes were drilled along the center line of the ridge top, with a plant spacing of 0.55m. After drilling, the tobacco seedlings were planted vertically, and then watered quickly, 1kg of water per hole. Once there was no standing water in the holes, the roots were surrounded with 3cm of supplementary soil.

[0034] The specific methods for each example are as follows:

[0035] Example 1

[0036] Weigh out the following combination regulator: γ-polyglutamic acid 60 kg / hm 2 Potassium humate from mineral sources 60 kg / hm 2 60 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.8 kg / hm 2 .

[0037] During the tobacco seedling stage (20 days after transplanting), weigh 36 kg / hm² of γ-polyglutamic acid. 2 Potassium humate from mineral sources 36 kg / hm 2 36 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.08 kg / hm 2After mixing the combined regulators, dilute with 4320 kg / hm of water. 2 Apply the acupoint treatment.

[0038] During the vigorous growth period of flue-cured tobacco (42 days after transplanting), weigh 24 kg / hm² of γ-polyglutamic acid. 2 Potassium humate from mineral sources 24 kg / hm 2 24 kg / hm² of deep-sea fish protein fertilizer 2 And phosphoenol pyruvate 0.72kg / hm 2 After mixing the combined regulators, dilute with 2880 kg / hm of water. 2 Apply the acupoint treatment.

[0039] Example 2

[0040] The combined regulator weighed out as follows: γ-polyglutamic acid 57 kg / hm 2 Potassium humate from mineral sources: 57 kg / hm 2 57 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.71 kg / hm 2 .

[0041] During the tobacco seedling stage (20 days after transplanting), weigh 34.2 kg / hm² of γ-polyglutamic acid. 2 Potassium humate from mineral sources: 34.2 kg / hm 2 34.2 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.026 kg / hm 2 After mixing the combined regulators, dilute with water at a rate of 4104 kg / hm². 2 Apply the acupoint treatment.

[0042] During the vigorous growth period of flue-cured tobacco (42 days after transplanting), 21.6 kg / hm² of γ-polyglutamic acid was weighed. 2 Potassium humate from mineral sources: 21.6 kg / hm 2 Deep-sea fish protein fertilizer 21.6 kg / hm 2 And phosphoenol pyruvate 0.648 kg / hm 2 After mixing the combined regulators, dilute with 2736 kg / hm of water. 2 Apply the acupoint treatment.

[0043] Example 3

[0044] The combined regulator was weighed as follows: γ-polyglutamic acid 54 kg / hm 2 Potassium humate from mineral sources: 54 kg / hm 2 54 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.62 kg / hm 2 .

[0045] During the tobacco seedling stage (20 days after transplanting), weigh 32.4 kg / hm² of γ-polyglutamic acid. 2 Potassium humate from mineral sources: 32.4 kg / hm 2 32.4 kg / hm² of deep-sea fish protein fertilizer 2 And phosphoenol pyruvate 0.972 kg / hm 2 After mixing the combined regulators, dilute with water at a rate of 4104 kg / hm². 2 Apply the acupoint treatment.

[0046] During the vigorous growth period of flue-cured tobacco (42 days after transplanting), 21.6 kg / hm² of γ-polyglutamic acid was weighed. 2 Potassium humate from mineral sources: 21.6 kg / hm 2 Deep-sea fish protein fertilizer 21.6 kg / hm 2 And phosphoenol pyruvate 0.648 kg / hm 2 After mixing the combined regulators, dilute with 2736 kg / hm of water. 2 Apply the acupoint treatment.

[0047] Example 4

[0048] The combined regulator was weighed as follows: γ-polyglutamic acid 51 kg / hm 2 Potassium humate from mineral sources 51 kg / hm 2 51 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.53 kg / hm 2 .

[0049] During the tobacco seedling stage (20 days after transplanting), weigh 30.6 g / hm of γ-polyglutamic acid. 2 Potassium humate from mineral sources: 30.6 kg / hm 2 30.6 kg / hm² of deep-sea fish protein fertilizer 2 And phosphoenol pyruvate 0.918 kg / hm 2 After mixing the combined regulators, dilute with water at a rate of 3888 kg / hm². 2 Apply the acupoint treatment.

[0050] During the vigorous growth period of flue-cured tobacco (42 days after transplanting), weigh 20.4 kg / hm² of γ-polyglutamic acid. 2 Potassium humate from mineral sources: 20.4 kg / hm 2 Deep-sea fish protein fertilizer 20.4 kg / hm 2 And phosphoenol pyruvate 0.612 kg / hm 2 After mixing the combined regulators, dilute with 2592 kg / hm of water. 2 Apply the acupoint treatment.

[0051] Example 5

[0052] The combined regulator weighed out as follows: γ-polyglutamic acid 48 kg / hm 2 Potassium humate from mineral sources 48 kg / hm 2 48 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.44 kg / hm 2 .

[0053] During the tobacco seedling stage (20 days after transplanting), weigh 28.8 g / hm of γ-polyglutamic acid. 2 Potassium humate from mineral sources: 28.8 kg / hm 2 Deep-sea fish protein fertilizer 28.8 kg / hm 2 And phosphoenol pyruvate 0.864 kg / hm 2 After mixing the combined regulators, dilute with water at a rate of 3672 kg / hm². 2 Apply the acupoint treatment.

[0054] During the vigorous growth stage of flue-cured tobacco (42 days after transplanting), 19.2 kg / hm² of γ-polyglutamic acid was weighed. 2 Potassium humate from mineral sources: 19.2 kg / hm 2 Deep-sea fish protein fertilizer 19.2g / hm 2 And phosphoenol pyruvate 0.576 kg / hm 2 After mixing the combined regulators, dilute with 2448 kg / hm of water. 2 Apply the acupoint treatment.

[0055] Example 6

[0056] In this embodiment, except that γ-polyglutamic acid is not added to the combined regulator, and the reduced portion is allocated proportionally to potassium humate from mineral sources, deep-sea fish protein fertilizer, and phosphoenol pyruvate (treated according to the method during both the crowning and vigorous growth stages), the rest is the same as in Example 1.

[0057] Example 7

[0058] In this embodiment, except that no mineral-derived potassium humate is added to the combined regulator, and the reduced portion is allocated proportionally to γ-polyglutamic acid, deep-sea fish protein fertilizer, and phosphoenol pyruvate (treated according to the method during both the crowning and vigorous growth stages), the rest is the same as in Example 1.

[0059] Example 8

[0060] In this embodiment, except that deep-sea fish protein fertilizer is not added to the combined regulator, and the reduced portion is allocated proportionally to mineral-derived potassium humate, γ-polyglutamic acid, and phosphoenolpyruvic acid (treated according to this method during both the basal and vigorous growth stages), the rest is the same as in Example 1.

[0061] Example 9

[0062] In this embodiment, except that phosphoenol pyruvate is not added to the combined regulator, and the reduced portion is allocated proportionally to potassium humate from mineral sources, deep-sea fish protein fertilizer, and γ-polyglutamic acid (treated according to the method during both the crowning stage and the vigorous growth stage), the rest is the same as in Example 1.

[0063] Example 10

[0064] In this embodiment, except for the following steps, everything else is the same as in Embodiment 1:

[0065] During the tobacco seedling stage (20 days after transplanting), weigh 24 kg / hm² of γ-polyglutamic acid. 2 Potassium humate from mineral sources 24 kg / hm 2 24 kg / hm² of deep-sea fish protein fertilizer 2 And phosphoenol pyruvate 0.72kg / hm 2 After mixing the combined regulators, dilute with 2880 kg / hm of water. 2 Apply the acupoint treatment.

[0066] During the vigorous growth period of flue-cured tobacco (42 days after transplanting), weigh 36 kg / hm² of γ-polyglutamic acid. 2 36 kg / hm of mineral-derived potassium humate 2 36 kg / hm² of deep-sea fish protein fertilizer 2 Phosphoenol pyruvate 1.08 kg / hm 2 After mixing the combined regulators, dilute with 4320 kg / hm of water. 2 Apply the acupoint treatment.

[0067] Effect test:

[0068] 1. Aroma component detection: Take tobacco leaves with a grade of C3F after curing (C3F: grade classification of flue-cured tobacco, C: middle leaf; F: orange-yellow color after curing; 3: grade; abbreviated as middle orange three; is a typical representative part and grade of tobacco leaf detection), divide them into two parts, one part for aroma component detection and the other part for sensory quality detection.

[0069] Detection method: The analysis was performed using a gas chromatography-mass spectrometry (GC-MS) system 8890-7000D (internal standard method for quantification, and the relative content of each component was calculated according to the formula).

[0070] Sample pretreatment: The samples were ground in liquid nitrogen and vortexed to mix evenly. 500 mg of each sample was weighed and placed in a headspace sample vial. 2 mL of saturated NaCl solution and 10 μL of 3-hexanone internal standard solution (50 μg / mL) were added. The samples were extracted using fully automated headspace solid phase microextraction (HS-SPME). The extract was then used for GC-MS analysis.

[0071] The specific steps for GC determination are as follows:

[0072] HS-SPME conditions: Under constant temperature of 60℃, shake for 5 min, insert a 120μm DVB / CWR / PDMS extraction head into the sample headspace vial, perform headspace extraction for 15 min, and then desorb at 250℃ for 5 min.

[0073] GC conditions: Inlet temperature: 250℃; Split mode: Splitless; Carrier gas: Helium (≥99.999%); Carrier gas flow rate: Constant flow, 1.2 mL / min; Solvent delay: 3.5 min.

[0074] Temperature program: Start at 40℃, hold for 3.5 min, increase to 100℃ at 10℃ / min, then increase to 180℃ at 7℃ / min, and finally increase to 280℃ at 25℃ / min, hold for 5 min.

[0075] Mass spectrometry conditions: Ion source: Electron impact (EI) source; Electron energy: 70 eV; Ion source temperature: 230℃; Quadrupole temperature: 150℃; Mass spectrometry interface temperature: 280℃; Scanning mode: Select ion detection mode (SIM) for precise qualitative and quantitative ion scanning.

[0076] The tobacco leaves obtained from the planting methods of Examples 1-10 and the control group (without any added combination regulators compared to Example 1) were tested, and the results are as follows:

[0077] Table 1

[0078]

[0079] 2. Sensory quality

[0080] The above-mentioned tobacco leaves were taken, shredded, and sampled. National experts in tobacco sensory quality assessment were engaged to conduct sensory quality testing and analysis. Sensory quality was assessed using a percentage-based evaluation, and the evaluation criteria are as follows:

[0081] Score / Indicator Fragrance style and degree of prominence flue gas concentration energy 3.6～5.0 powerful concentrated big 2.1～3.5 Strong Concentrated Larger 1.6～2.0 medium medium medium 0.1～1.5 Weak Lighter smaller ≤0.1 weak light Small

[0082] Score / Indicator Fragrance Aroma Mixed gases Irritating Aftertaste 7.6～9.0 Good, relatively good sufficient, relatively sufficient light Small Comfort 6.1～7.5 Upper-middle Still enough Lighter smaller More comfortable 4.6～6.0 medium have have have Comfortable 3.1～4.5 Lower middle less heavier Larger Uncomfortable ≤3.0 Poor few Heavy big tongue

[0083] The results are as follows:

[0084] Table 2

[0085]

[0086]

[0087] As shown in Table 1, after using the combined regulator of this invention, the levels of phenylethyl alcohol, phenylacetaldehyde, phenylacetic acid, and methyl phenylacetate, which are phenylalanine compounds, were significantly increased. Phenylacetic acid imparts a sweet and honey-like aroma to tobacco, while phenylacetaldehyde has a honey and rose-like aroma. Furthermore, the aroma-modifying component of 2-phenylethyl alcohol, phenylacetic acid, has a honey-sweet aroma and plays a positive role in enhancing the honey-sweet aroma. The application of the combined regulator significantly increased the levels of these substances, which is beneficial for improving the overall aroma content of flue-cured tobacco, enhancing its sensory quality, and particularly strengthening the honey-sweet aroma of Bijie tobacco leaves.

[0088] As shown in Table 2, the sensory quality of the flue-cured tobacco leaves was improved after applying the combined regulators, effectively enhancing the sensory quality of the flue-cured tobacco in the examples. The total scores were all higher than the control group, with the increase in the total sensory quality score of the flue-cured tobacco in the examples ranging from 6.21% to 11.37%, indicating a significant improvement in the sensory quality of the flue-cured tobacco in the examples. This invention, by applying combined regulators to flue-cured tobacco during the rosette stage and vigorous growth stage, can increase the photosynthetic capacity of tobacco leaves, improve chlorophyll synthesis, enhance the stress resistance of tobacco seedlings, innovate tobacco aroma enhancement technology, promote the absorption of nutrients by tobacco leaves and their conversion into aroma substances, and further improve the sensory quality of tobacco leaves.

[0089] Furthermore, data from Examples 1 and 6-10 show that the present invention, by using a compound of mineral-derived potassium humate, γ-polyglutamic acid, deep-sea fish protein fertilizer, and phosphoenol pyruvate, works synergistically and is combined with a specific application process to further improve the sensory quality and aroma of tobacco leaves.

[0090] The applicant declares that this invention illustrates the combined regulator for improving the sensory quality of tobacco leaves and its application through the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of the raw materials of this invention, addition of auxiliary components, and selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0091] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0092] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A combination regulator for improving the sensory quality of tobacco leaves, characterized in that, The combined regulator comprises, by weight, 30-60 parts of mineral-derived potassium humate, 30-60 parts of γ-polyglutamic acid, 30-60 parts of deep-sea fish protein fertilizer, and 0.9-1.8 parts of phosphoenol pyruvate.

2. The combined regulator for improving the sensory quality of tobacco leaves according to claim 1, characterized in that, The combined regulator comprises, by weight, 35-55 parts of mineral-derived potassium humate, 35-55 parts of γ-polyglutamic acid, 35-55 parts of deep-sea fish protein fertilizer, and 1.05-1.65 parts of phosphoenol pyruvate.

3. The application of a combined regulator for improving the sensory quality of tobacco leaves according to claim 1 or 2 in tobacco cultivation.

4. A method for cultivating tobacco, characterized in that, In the tobacco planting method, the combined regulator for improving the sensory quality of tobacco leaves as described in claim 1 or 2 is added during topdressing.

5. The tobacco planting method according to claim 4, characterized in that, The total addition amount of the combined regulator is 91.5-183 kg / hm. 2 .

6. The tobacco cultivation method according to claim 4 or 5, characterized in that, The process of adding the combined regulator for improving the sensory quality of tobacco leaves as described in claim 1 or 2 during fertilization is specifically as follows: The combined regulator was mixed with water and applied as a hole irrigation during the seedling stage and the vigorous growth stage, respectively.

7. The tobacco planting method according to claim 6, characterized in that, The mass ratio of the combined regulator used during the budding stage and the vigorous growth stage is (2.7-3.3):(1.7-2.3).

8. The tobacco cultivation method according to any one of claims 4-7, characterized in that, The mass ratio of the combined regulator to water is 1:(30-50).

9. The tobacco cultivation method according to any one of claims 4-8, characterized in that, The sown period is 18-24 days after the tobacco seedlings are transplanted.

10. The tobacco cultivation method according to any one of claims 4-9, characterized in that, The vigorous growth period is 42-48 days after the tobacco seedlings are transplanted.

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