Tea product for relieving gout and conditioning endocrine balance and preparation method thereof

Abstract

The invention belongs to the technical field of tea making, and discloses a tea product capable of relieving gout and conditioning endocrine balance and a preparation method of the tea product capable of relieving gout and conditioning endocrine balance. The health-care tea consists of 20-40 parts of tea leaves, 20-30 parts of roses, 10-20 parts of lalang grass rhizome, 10-15 parts of coix seed buds, 8-15 parts of semen plantaginis, 6-12 parts of lily polysaccharide, 5-10 parts of chicory, 4-8 parts of ligusticum chuanxiong hort, 3-7 parts of angelica sinensis, 2-4 parts of liquorice, 2-3 parts of dipotassium phosphate and 1-2 parts of sodium bicarbonate. The preparation method of the tea product capable of relieving gout and conditioning endocrine balance is simple and easy to implement, the prepared tea product has homology of medicine and food, the tea leaves are reasonably matched with the roses, the lalang grass rhizome, the coix seed buds, the semen plantaginis, the lily polysaccharide, the endive, the ligusticum chuanxiong hort, the angelica sinensis and the liquorice, the endocrine balance can be conditioned, early intervention of gout is achieved, and the effect of relieving gout is achieved. Diseases are prevented from developing to the end stage.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of tea making, and particularly relates to a tea product for relieving gout and regulating endocrine balance and a preparation method thereof. BACKGROUND

[0002] At present, gout is a common and complex type of arthritis, and people of all ages can suffer from this disease, with a higher incidence in men than in women. Gout patients often experience sudden joint pain at night, with acute onset, severe pain, edema, redness and inflammation at the joint site, and the pain gradually subsides until it disappears, lasting for several days or weeks. Gout is a crystal-related arthropathy caused by monosodium urate (MSU) deposition, and is directly related to hyperuricemia caused by purine metabolism disorder and / or reduced uric acid excretion.

[0003] Hyperuricemia is the basis for the occurrence of gout, and hyperuricemia and excessive saturation of uric acid in urine cause urate to deposit in the renal tubular lumen or interstitium, leading to acute inflammatory response. At present, the drugs for relieving gout mainly include non-steroidal anti-inflammatory drugs and glucocorticoids, which have short-term efficacy but high recurrence rate and strong dependence, and have obvious toxic and side effects when taken for a long time.

[0004] Puckowska et al. disclosed in patent CN111034835A a tea product for relieving gout and regulating endocrine balance, which is mainly made of white orchid root, honeysuckle, osmanthus, dandelion and honey. The process only involves mixing the medicinal materials according to the weight parts and directly packaging, without involving the enrichment or extraction of active ingredients or any optimization of the dissolution efficiency and stability of the components. The whole process stays at the most basic level of "mixing - sterilization - packaging", without special extraction of lily polysaccharides and active substances of coix seed sprouts, and without using modernized fractional filtration, concentration or enzymatic steps, so that the content of effective ingredients in the finished product is difficult to quantify and standardize.

[0005] It can be seen that the technical solution has obvious limitations: on the one hand, various functional ingredients are mostly in the form of raw material coarse powder, with low water solubility and bioavailability, which is difficult to meet the demand for rapid effect in clinical or large-scale drinking; on the other hand, the process lacks fine control of the pretreatment of raw materials, optimization of extraction carriers and proportioning of excipients, resulting in large quality fluctuations between batches of products and difficulty in matching the industrialized production standards of modern health tea. At the same time, the simple reliance on mixing of medicinal materials also fails to balance the taste and function, limiting the popularization and application of the formula in a wider population. SUMMARY

[0006] In view of the problems existing in the prior art, the present application provides a tea product for relieving gout and regulating endocrine balance and a preparation method thereof.

[0007] The present application is achieved in a kind of tea products for relieving gout and regulating endocrine balance, the tea products for relieving gout and regulating endocrine balance are made of tea leaf 20-40 parts, rose 20-30 parts, white cana 10-20 parts, yiyi bud 10-15 parts, psyllium 8-15 parts, lily polysaccharide 6-12 parts, chicory 5-10 parts, chuanqiong 4-8 parts, angelica 3-7 parts, licorice 2-4 parts, di-potassium hydrogen phosphate 2-3 parts and sodium bicarbonate 1-2 parts.

[0008] Another purpose of the present application is to provide a kind of tea products for relieving gout and regulating endocrine balance for preparing the tea products for relieving gout and regulating endocrine balance, the preparation method of the tea products for relieving gout and regulating endocrine balance includes the following steps:

[0009] Step one, carry out the pretreatment of tea leaf: after screening, fresh tea leaf is laid in the airing room and is laid to the moisture content of 60-70%;

[0010] Step two, preparation of yiyi bud: after washing clean, yiyi is soaked in water and germinated;After washing the germinated yiyi, it is dried, crushed, sieved, shelled, and yiyi bud powder is obtained;Add distilled water, saccharify with saccharifying enzyme, filter, take the filtrate, concentrate, spray dry, and yiyi bud is obtained;

[0011] Step three, preparation of lily polysaccharide: after removing impurities, lily is washed, crushed, enzyme inactivation and deodorization, and filtered;After degreasing, add distilled water and adjust pH, and extract in water bath;Enzymatic hydrolysis, centrifugation, concentration, vacuum freeze-drying;After deproteinization, decolorization and dialysis, vacuum freeze-drying, lily polysaccharide is obtained;

[0012] Step four, preparation of composite tea product: preparation of white cana extract;After washing, baking and crushing, rose, psyllium, chicory, chuanqiong, angelica and licorice are mixed with tea leaf, white cana extract, lily polysaccharide, white cana and di-potassium hydrogen phosphate and sodium bicarbonate, sterilized, packaged, and the tea product for relieving gout and regulating endocrine balance is obtained.

[0013] Further, the pretreatment method of tea leaf in step one includes:

[0014] (1) screening the collected fresh tea leaf, removing old leaves, yellow leaves and damaged tea leaves, and laying the screened tea leaf in the airing room to 60-70% moisture content;

[0015] (2) The tea leaves obtained by spreading and airing are put into a fixation machine to fix the tea, the fixation temperature and time are adjusted, the fixation is performed until the moisture content is 40-50%, and the fixed tea is cooled to room temperature to obtain fixed tea leaves;

[0016] (3) The fixed tea leaves are put into a rolling machine to roll the tea leaves, and the rolled tea leaves are put into a drying device to dry the tea leaves, to obtain pretreated tea leaves.

[0017] Further, the rolling machine in step (3) has a rotating speed of 25-30 r / min, and the rolling time is 10-15 min.

[0018] Further, the preparation method of the coix seed sprouts in step two comprises:

[0019] (1) The coix seeds are washed with clean water for 1-3 times; after being washed clean, the coix seeds are soaked in water at 25-37℃ for 12-24 h, and germinated at room temperature for 2-4 days to obtain germinated coix seeds;

[0020] (2) The germinated coix seeds obtained in step (1) are washed with roots, and then dried at 40-60℃, crushed and sieved through a 80-120 mesh sieve, and shelled to obtain coix seed sprout powder;

[0021] (3) The coix seed sprout powder obtained in step (2) is added into distilled water at a mass ratio of 1:1, and saccharifying enzyme is added for saccharification, filtration, concentration of the filtrate, and spray drying to obtain coix seed sprouts.

[0022] Further, the saccharifying enzyme in step (3) is 2-4% of the mass of the coix seed sprout solution.

[0023] Further, the preparation method of the lily polysaccharide in step three comprises:

[0024] (1) After the impurities in the lily are removed, the lily is washed with normal temperature water for 2-4 times by using a vegetable washing machine, and crushed into a slurry by using a crusher, and subjected to enzyme inactivation at 50-65℃ and vacuum deodorization, filtration, and precipitation to obtain lily wet starch;

[0025] (2) The lily wet starch obtained in step (1) is defatted with ethyl acetate and petroleum ether respectively, added into distilled water, and adjusted to pH 5.0-6.0, and subjected to water bath extraction, and the supernatant is taken;

[0026] (3) After the supernatant is cooled, the starch is removed by enzymatic hydrolysis, and the supernatant is obtained by centrifugation; after the supernatant is concentrated, 2-4 times the volume of 95% ice ethanol is added to precipitate overnight, and the precipitate is vacuum freeze-dried to obtain lily crude extract;

[0027] (4) The lily crude extract obtained in step (3) is dissolved into a solution by adding water, subjected to deproteinization, decolorization, dialysis and ion exchange column chromatography, and vacuum freeze-dried again to obtain lily polysaccharide.

[0028] Further, the distilled water in step (2) is added in an amount of 1:20-40;

[0029] The water bath extraction temperature is 80-95℃, and the water bath extraction time is 3-6h.

[0030] Further, the preparation method of the composite tea product in step four comprises:

[0031] (1) tea, rose, white flag, seed of job's tears, plantain, lily polysaccharide, chicory, chuanqiong, angelica, licorice, dipotassium hydrogen phosphate and sodium bicarbonate are weighed according to the mass fraction, respectively, and prepared for use;

[0032] (2) the white flag is crushed and sieved, then extracted with ethanol reflux, the obtained extract is successively extracted with petroleum ether, ethyl acetate and n-butanol, the raffinate is evaporated and dried to obtain the white flag extract;

[0033] (3) the rose, plantain, chicory, chuanqiong, angelica and licorice are placed in a vegetable washing machine, and 10% pesticide residue cleaning solution is added to the water at 55-65℃ for cleaning, then washed with clean water for 2-4 times;

[0034] (4) the cleaned raw materials obtained in step (3) are baked in a hot air rotary furnace at 60-70℃ for 2-3h, and are crushed to 160 mesh fine powder, respectively, for standby;

[0035] (5) the rose powder, plantain powder, chicory powder, chuanqiong powder, angelica powder and licorice powder obtained in step (4) are mixed with the pretreated tea, white flag extract, lily polysaccharide, white flag and dipotassium hydrogen phosphate and sodium bicarbonate, sterilized, packaged 10g / pack, to obtain the composite tea product.

[0036] Further, the preparation method of the white flag extract in step (2) comprises:

[0037] 1) the white flag is crushed and sieved through a 100-120 mesh sieve, then extracted with 50-90wt% ethanol water solution at 80-85℃ for 1-3h, the obtained extract is concentrated to obtain the extract;

[0038] 2) the extract obtained in step 1) is suspended with water, then extracted with petroleum ether, the water phase obtained by extraction is extracted with ethyl acetate, and the water phase obtained after ethyl acetate extraction is extracted with n-butanol;

[0039] 3) the raffinate obtained after n-butanol extraction in step 2) is evaporated and dried to obtain the white flag extract.

[0040] In combination with the above technical solutions and the technical problems solved, the technical solutions to be protected by the present application have the advantages and positive effects of:

[0041] The tea product for relieving gout and regulating endocrine balance provided by the present application is prepared by reasonably matching tea leaves with rose, rhizoma imperatae, coix seed sprout, psyllium, lily polysaccharide, chicory, chuanqiong, angelica and licorice, which can remove toxins in the body, effectively enhance the resistance, achieve the purpose of relieving gout and regulating endocrine balance.

[0042] Among them, rhizoma imperatae is sweet, cold, and belongs to lung, stomach and bladder channels, which has the effects of cooling blood to stop bleeding and clearing heat to induce diuresis; chicory is slightly bitter and salty, and is cold, and belongs to liver, gallbladder and stomach channels, which has the effects of clearing liver and gallbladder, invigorating stomach and food digestion, diuresis and detumescence; coix seed is cool, and is sweet and mild, and belongs to spleen, stomach and lung channels, which has the effects of water diuresis and detumescence, spleen tonification and middle energizing, spleen dampness removing, spleen tonification and diarrhea stopping, dampness removing and arthralgia relieving, and can relax tendons and relieve spasm.

[0043] Rose is used for liver and stomach qi pain, food aversion and vomiting, irregular menstruation, sprain and pain, promoting qi circulation and relieving depression, and blood circulation and pain relief, and it is recorded in Bencao Zhengj i that rose has the strongest fragrance, is clear and not turbid, is harmonious and not violent, can relax the liver and invigorate the stomach, and can promote qi and activate blood without the side effects of pungent, warm, harsh and dry, and is the most effective and gentle among qi partition drugs, and the fragrance of rose is unmatched by other products.

[0044] Chuanqiong can activate blood and remove stasis, and can also promote qi and relieve pain, has the effect of unblocking qi and blood, and is used for treating chest and abdominal pain caused by qi stagnation and blood stasis, and can also dispel wind and relieve pain, and is a main medicine for treating headache, and lingzhi has different degrees of effects on neurasthenia, hyperlipidemia, angina pectoris, arrhythmia, Keshan disease, high altitude discomfort, hepatitis, hemorrhagic fever, indigestion, and bronchitis.

[0045] The preparation method of the tea product for relieving gout and regulating endocrine balance provided by the present application is simple and easy to operate, the prepared tea product is homologous in medicine and food, the matching is scientific, can effectively improve the onset of gout, regulate endocrine balance, achieve early intervention of gout, and avoid the development of the disease to the terminal stage. BRIEF DESCRIPTION OF DRAWINGS

[0046] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiments of the present application will be briefly introduced as follows. Obviously, the drawings described below are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0047] Figure 1 is a preparation method flow chart of the tea product for relieving gout and regulating endocrine balance provided by the present application;

[0048] Figure 2 is a pretreatment method flow chart of tea leaves provided by the present application;

[0049] Figure 3 Figure is a flow chart of a preparation method of the coix seed sprout provided by the embodiment of the present application. DETAILED DESCRIPTION

[0050] In order to make the objectives, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and not used to limit the present application.

[0051] In view of the problems in the prior art, the present application provides a tea product for relieving gout and regulating endocrine balance and a preparation method thereof, which will be described in detail below with reference to the drawings.

[0052] The tea product for relieving gout and regulating endocrine balance provided by the embodiment of the present application is composed of tea leaves 20-40 parts, rose flowers 20-30 parts, white flag grass 10-20 parts, coix seed sprouts 10-15 parts, psyllium 8-15 parts, lily polysaccharide 6-12 parts, chicory 5-10 parts, chuanxiong 4-8 parts, angelica 3-7 parts, licorice 2-4 parts, di-potassium hydrogen phosphate 2-3 parts and sodium bicarbonate 1-2 parts in terms of mass fraction.

[0053] As shown in Figure 1 The preparation method of the tea product for relieving gout and regulating endocrine balance provided by the embodiment of the present application includes the following steps:

[0054] S101, performing pretreatment of tea leaves: after screening, the fresh tea leaves are laid flat in a spreading room for spreading; after the spread tea leaves are put into a fixation machine for fixation, they are put into a rolling machine for rolling; the rolled tea leaves are put into a drying device for drying to obtain pretreated tea leaves;

[0055] S102, preparing coix seed sprouts: the coix seeds are cleaned with clean water and then soaked in water for germination; the germinated coix seeds are washed with roots, dried, crushed, sieved and shelled to obtain coix seed sprout powder; distilled water and saccharifying enzyme are added for saccharification, filtration, concentration of the filtrate, and spray drying to obtain coix seed sprouts;

[0056] S103, preparing lily polysaccharide: the lily is cleaned after removing impurities, crushed, enzyme-inactivated and deodorized, and filtered; after degreasing, the lily is added into distilled water and the pH is adjusted, and then extracted by water bath; enzyme hydrolysis, centrifugation, concentration, vacuum freeze-drying, deproteinization, decolorization, dialysis and vacuum freeze-drying are performed to obtain lily polysaccharide;

[0057] S104, preparing the compound tea product: preparing the Imperata cylindrical extract; washing, baking, and crushing the rose, psyllium, chicory, Rhizoma et Radix Notoginseng, angelica and licorice, mixing with tea, the Imperata cylindrical extract, lily polysaccharide, Imperata cylindrical and dipotassium hydrogen phosphate and sodium bicarbonate, sterilizing, packaging, and obtaining the tea product for relieving gout and regulating endocrine balance.

[0058] As shown in Figure 2 The preprocessing method of the tea in step S101 provided by the embodiment of the application comprises:

[0059] S201, screening the collected fresh tea leaves, removing old leaves, yellow leaves and damaged tea leaves, and laying the screened tea leaves in a spreading room until the moisture content is 60-70%;

[0060] S202, putting the spread tea leaves into a fixation machine to fix the tea leaves, adjusting the fixation temperature and time, fixing the tea leaves until the moisture content is 40-50%, and cooling to room temperature to obtain fixed tea leaves;

[0061] S203, putting the fixed tea leaves into a rolling machine to roll the tea leaves, and putting the rolled tea leaves into a drying device to dry the tea leaves, and obtaining the preprocessed tea leaves.

[0062] The rolling machine in step S203 provided by the embodiment of the application has a rotating speed of 25-30 r / min and a rolling time of 10-15 min.

[0063] As shown in Figure 3 The preparation method of the barley sprout in step S102 provided by the embodiment of the application comprises:

[0064] S301, washing the barley with clean water for 1-3 times; after washing, soaking the barley in water at 25-37 DEG C for 12-24 h, and germinating the barley at room temperature for 2-4 days to obtain the germinated barley;

[0065] S302, washing the germinated barley obtained in S301, drying the moisture of the germinated barley at 40-60 DEG C, crushing and passing through a 80-120 mesh sieve, and removing the hull to obtain the barley sprout powder;

[0066] S303, adding distilled water to the barley sprout powder obtained in S302 according to a mass ratio of 1:1, adding saccharifying enzyme for saccharification, filtering, concentrating the filtrate, and spray drying to obtain the barley sprout.

[0067] The saccharifying enzyme in step S303 provided by the embodiment of the application is 2-4% of the mass of the barley sprout powder solution.

[0068] The preparation method of the lily polysaccharide in step S103 provided by the embodiment of the application comprises:

[0069] (1) after removing impurities in lily, the lily is washed with normal temperature water for 2-4 times by using a vegetable washing machine, and is crushed into slurry by using a crusher, and is subjected to enzyme inactivation at 50-65 DEG C and vacuum deodorization, filtration, and precipitation to obtain lily wet starch;

[0070] (2) after removing impurities in lily, the lily is washed with normal temperature water for 2-4 times by using a vegetable washing machine, and is crushed into slurry by using a crusher, and is subjected to enzyme inactivation at 50-65 DEG C and vacuum deodorization, filtration, and precipitation to obtain lily wet starch;

[0071] (3) after removing impurities in lily, the lily is washed with normal temperature water for 2-4 times by using a vegetable washing machine, and is crushed into slurry by using a crusher, and is subjected to enzyme inactivation at 50-65 DEG C and vacuum deodorization, filtration, and precipitation to obtain lily wet starch;

[0072] (4) after removing impurities in lily, the lily is washed with normal temperature water for 2-4 times by using a vegetable washing machine, and is crushed into slurry by using a crusher, and is subjected to enzyme inactivation at 50-65 DEG C and vacuum deodorization, filtration, and precipitation to obtain lily wet starch;

[0073] The adding amount of distilled water in step (2) provided by the embodiment of the application is 1:20-40; the water bath extraction temperature is 80-95 DEG C, and the water bath extraction time is 3-6 h.

[0074] The preparation method of the composite tea product in step S104 provided by the embodiment of the application comprises:

[0075] (1) tea leaves, rose flowers, white orris root, seed of Job's tears, psyllium, lily polysaccharide, chicory, chuanqiong, angelica, licorice, dipotassium hydrogen phosphate and sodium bicarbonate are respectively weighed according to mass fraction, and are prepared for use;

[0076] (2) the white orris root is crushed and sieved, and is extracted by refluxing with ethanol, the obtained extract is sequentially extracted with petroleum ether, ethyl acetate and n-butanol, the raffinate is evaporated and dried to obtain white orris root extract;

[0077] (3) the rose flowers, psyllium, chicory, chuanqiong, angelica and licorice are placed in a vegetable washing machine, and are washed by adding 10% pesticide residue cleaning solution into water at 55-65 DEG C, and then are washed with clean water for 2-4 times;

[0078] (4) the washed raw materials obtained in step (3) are respectively baked in a hot air rotary furnace at 60-70 DEG C for 2-3 h, and are respectively crushed into fine powder of 160 mesh, and are prepared for use;

[0079] (5) the rose flower powder, psyllium powder, chicory powder, chuanqiong powder, angelica powder and licorice powder obtained in step (4) are mixed with pretreated tea leaves, white orris root extract, lily polysaccharide, white orris root, dipotassium hydrogen phosphate and sodium bicarbonate, sterilized, packaged 10 g per bag to obtain a composite tea product.

[0080] The preparation method of the Imperata cylindrical extract in step (2) provided by the embodiment of the present application comprises:

[0081] 1) crushing the Imperata cylindrical, passing through a 100-120 mesh sieve, and then refluxing and extracting the Imperata cylindrical with 50-90 wt% ethanol water solution at 80-85 DEG C for 1-3 hours, concentrating the obtained extract to obtain an extract;

[0082] 2) suspending the extract obtained in step 1) with water, and then extracting with petroleum ether, extracting the obtained water phase with ethyl acetate again, and then extracting the obtained water phase with n-butanol again;

[0083] 3) evaporating and drying the residue obtained after the n-butanol extraction in step 2) to obtain the Imperata cylindrical extract.

[0084] The tea product for relieving gout and regulating endocrine balance provided by the present application is prepared by reasonably matching tea leaves with rose, Imperata cylindrical, coix seed, psyllium, lily polysaccharide, chicory, chuanqiong, angelica and licorice, which can remove toxins in the body, effectively enhance the resistance, and achieve the purposes of relieving gout and regulating endocrine balance.

[0085] The preparation method of the tea product for relieving gout and regulating endocrine balance provided by the present application is simple and easy to operate, and the prepared tea product is homologous in medicine and food, and is reasonably matched, which can effectively improve the onset of gout, regulate endocrine balance, achieve early intervention of gout, and avoid the development of the disease to the terminal stage.

[0086] The Imperata cylindrical is sweet, cold, and belongs to the lung, stomach and bladder channels, and has the effects of cooling blood and stopping bleeding, and clearing heat and diuresis; the chicory is slightly bitter and salty, and is cool, and belongs to the liver, gallbladder and stomach channels, and has the effects of clearing liver and gallbladder, invigorating stomach and digesting food, diuresis and detumescence; the coix seed is cool, and is sweet and mild, and belongs to the spleen, stomach and lung channels, and has the effects of water-eliminating and detumescence, spleen-invigorating and center-nourishing, spleen-dampness-eliminating, spleen-stomach-stopping, dampness-eliminating and arthralgia-relieving, and can relax the tendons and relieve the spasm.

[0087] The rose is used for liver and stomach qi pain, food aversion and vomiting, irregular menstruation, sprain and pain, and can promote qi circulation, relieve stagnation, and regulate blood, and stop pain, and it is recorded in the Bencao Zhengj i that the rose has the strongest fragrance, is clear and not turbid, is harmonious and not violent, can soothe the liver and stomach, promote qi circulation and activate blood, and has no side effects of pungent, warm, harsh and dry, and is the most effective and gentle among the qi partition drugs, and the aromatic products are almost unmatched.

[0088] The chuanqiong can activate blood and resolve stasis, and can also promote qi circulation and stop pain, has the effect of unblocking qi and blood, and is used for treating chest and abdomen pain caused by qi stagnation and blood stasis, and can also dispel wind and stop pain, and is a main medicine for treating headache, and the ganoderma lucidum has different degrees of effects on neurasthenia, hyperlipidemia, angina pectoris, arrhythmia, Keshan disease, high altitude discomfort, hepatitis, hemorrhagic fever, indigestion, and bronchitis.

[0089] Example 1

[0090] The tea leaves were pre-processed according to the first step of claim 1 to obtain tea semi-product, using the fresh leaves of the above Lngzheng tea as raw material. After the moisture content of the fresh leaves was reduced to 65%, the tea leaves were fixed at 90°C to a moisture content of 45%, rolled for 12 minutes, and dried to a moisture content of 5% to obtain tea powder with distinct white hairs and fresh aroma.

[0091] The tea powder was further mixed with the coix seed sprout powder (100 mesh) prepared according to claim 4, lily polysaccharide powder, white flag root extract, and auxiliary ingredients (rose, plantain, chicory, etc.) in a formula ratio, vacuum sterilized and packaged into 8g / pack composite tea. After sensory evaluation, the tea had golden yellow and bright soup, complex aroma, mellow taste, and no peculiar smell.

[0092] Example 2

[0093] In this example, the preferred glutinous coix seeds were soaked and germinated at 25°C for 16 hours according to the coix seed sprout process of claims 2-5, with a germination rate of 95%. The coix seeds were dried to a moisture content of 3%, crushed and sieved through an 80 mesh sieve to obtain fine and delicate white coix seed sprout powder.

[0094] The coix seed sprout powder was reacted with saccharifying enzyme (enzyme amount accounting for 3% of the mass of the coix seed sprout powder) in a 60°C water bath for 1 hour, filtered, concentrated, and spray dried to obtain coix seed sprout powder with an enzyme activity retention rate of more than 85%. The powder was light yellow, had good solubility after being suspended in water, and had no grainy feeling.

[0095] Example 3

[0096] In this example, the local seasonal lilies were selected according to the process of claims 6-7, washed, cleaned, crushed, and then enzyme-inactivated and deodorized at 60°C for 30 minutes to obtain viscous wet starch. After defatting, the lilies were extracted at a solid-liquid ratio of 1:30 at 90°C for 4 hours, the residue was removed by centrifugation, and the lilies were cooled and de-starched by enzymatic hydrolysis. The lilies were concentrated, precipitated with 4 volumes of ice ethanol, vacuum freeze-dried to obtain lily polysaccharide crude product.

[0097] The crude product was further treated by deproteinization, activated carbon decolorization, and 3kDa dialysis, and then freeze-dried again to obtain white and loose lily polysaccharide powder. The polysaccharide content was determined by the phenol-phosphoric acid method to be 92.4%, and the lily polysaccharide had good viscoelasticity and was suitable for compounding.

[0098] Example 4

[0099] In this example, the white flag root extract was prepared according to claims 8-9. Crushed white flag root to 120 mesh was extracted with 70% ethanol by reflux extraction for 2 hours. After concentration, stepwise extraction was performed with petroleum ether, ethyl acetate, and n-butanol to obtain yellow-brown viscous extract, which was dried to obtain white flag root extract.

[0100] The total saponin content in the extract is 6.8% determined by ultraviolet spectrophotometry, and the extract has significant diuresis and relieving stranguria effects. After mixing the series of powders in step 1-3 with the extract in a certain proportion, homogenizing with sodium bicarbonate and dipotassium hydrogen phosphate, sterilizing, the composite tea product is prepared. The HPLC fingerprint spectrum of the product is consistent with the standard, and the product meets the quality requirements.

[0101] Example 5

[0102] In this example, the efficacy of the composite tea is evaluated by using an acute high uric acid model of rats. 48 male SD rats are randomly divided into four groups (control, high uric acid model, low-dose tea, and medium-dose tea), and the corresponding solutions are administered intragastrically for 14 days. The results show that the serum uric acid level of the medium-dose group is reduced from 235±12 μmol / L of the model group to 142±10 μmol / L (P<0.01), which has a significant uric acid-lowering effect.

[0103] At the same time, the XO (xanthine oxidase) activity in the kidney tissue of the tea group is reduced by 48% compared with the model group, and the urine pH is observed to increase by 0.3 units, indicating that the product has a good promoting effect on regulating endocrine-acid-base balance.

[0104] Example 6

[0105] In this example, process comparison experiments are carried out for different tea bases: green tea, oolong tea, and black tea are each used as a carrier, and yiren sprouts, lily polysaccharides, and other medicinal materials are respectively compounded according to the preparation process of examples 1-3.

[0106] The sensory evaluation and dissolution experiment results show that oolong tea is the best choice for industrial production as the tea base, which can best coordinate the release rate of tea polyphenols and medicinal substances, and the soup color, aroma, and dissolution rate of medicinal ingredients are better than those of green tea and black tea.

[0107] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any modification, equivalent replacement, and improvement within the technical range disclosed in the present application, which is within the spirit and principles of the present application, should be covered within the protection scope of the present application.

Claims

1. A method for preparing a tea product that relieves gout and regulates endocrine balance, characterized in that, Includes the following steps: Step 1: Pre-treatment of tea leaves: After screening, fresh tea leaves are spread out in a drying room for drying; the dried tea leaves are then put into a fixing machine for fixing, and then into a rolling machine for rolling; the rolled tea leaves are then put into a drying device for drying, resulting in pre-treated tea leaves. Step 2: Preparation of Job's tears sprouts: Rinse Job's tears with clean water and soak them in water to germinate; wash the germinated Job's tears along with the roots, dry them, crush them, sieve them, remove the shells, and obtain Job's tears sprout powder; add distilled water, add saccharifying enzyme to saccharify, filter, concentrate the filtrate, and spray dry it to obtain Job's tears sprouts; Step 3: Preparation of lily polysaccharides: After removing impurities from the lily bulbs, wash, crush, deodorize and inactivate enzymes, and filter; after defatting, add distilled water and adjust the pH, extract in a water bath; enzymatically hydrolyze, centrifuge, concentrate, and freeze-dry under vacuum; after deproteinization, decolorization, dialysis, and freeze-drying under vacuum, lily polysaccharides are obtained. Step four, preparation of compound tea products: preparation of Imperata cylindrica root extract; after washing, baking and pulverizing rose petals, plantain seeds, chicory, Ligusticum chuanxiong, Angelica sinensis and licorice, mix them with tea leaves, Imperata cylindrica root extract, lily polysaccharide, Imperata cylindrica root, dipotassium hydrogen phosphate and sodium bicarbonate, sterilize, and package to obtain tea products that relieve gout and regulate endocrine balance.

2. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 1, characterized in that, The tea pretreatment method in step one includes: (1) The fresh tea leaves collected are screened to remove old leaves, yellow leaves and tea leaves with broken leaves. The screened tea leaves are spread out in the drying room until the moisture content is 60-70%. (2) Put the tea leaves obtained from the drying process into the fixing machine for fixing, adjust the fixing temperature and time, fix until the moisture content is 40-50%, cool to room temperature, and obtain the fixed leaves; (3) Put the tea leaves after killing the green into a rolling machine for rolling, and put the rolled tea leaves into a drying device for drying to obtain pre-treated tea leaves.

3. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 2, characterized in that, The speed of the kneading machine in step (3) is 25-30 r / min, and the kneading time is 10-15 min.

4. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 1, characterized in that, The preparation method of the Job's tears sprouts in step two includes: (1) Rinse the Job's tears with clean water 1 to 3 times; after cleaning, soak them in water at 25 to 37°C for 12 to 24 hours, and let them germinate at room temperature for 2 to 4 days to obtain sprouted Job's tears; (2) Take the sprouted coix seed obtained in step (1), wash it with the roots, dry it at 40-60℃, crush it and pass it through an 80-120 mesh sieve, remove the shell, and obtain coix seed sprout powder. (3) Add the Job's tears sprout powder obtained in step (2) to distilled water at a mass ratio of 1:1, add saccharifying enzyme to saccharify, filter, concentrate the filtrate, spray dry to obtain Job's tears sprout.

5. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 4, characterized in that, The saccharifying enzyme in step (3) is 2-4% of the mass of the coix seed sprout powder solution.

6. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 1, characterized in that, The preparation method of lily polysaccharide in step three includes: (1) After removing impurities from the lily, wash it 2-4 times with room temperature water using a vegetable washing machine, and then crush it into a slurry using a pulverizer. After enzyme inactivation and vacuum deodorization at 50-65℃, filter and precipitate to obtain lily wet starch. (2) After defatting the lily wet starch obtained in step (1) with ethyl acetate and petroleum ether respectively, add it to distilled water and adjust the pH to 5.0-6.0, extract in a water bath, and take the supernatant. (3) After cooling the supernatant, the starch is removed by enzymatic hydrolysis and centrifugation is performed to obtain the supernatant. After concentrating the supernatant, 2 to 4 times the volume of the concentrated liquid is added to 95% ice ethanol to precipitate overnight. The precipitate is then freeze-dried under vacuum to obtain crude lily extract. (4) Add water to the crude lily extract obtained in step (3) to dissolve it into a solution. After deproteinization, decolorization, dialysis and ion exchange column chromatography, it is freeze-dried under vacuum again to obtain lily polysaccharide.

7. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 6, characterized in that, The amount of distilled water added in step (2) is 1:20-40; The water bath extraction temperature is 80–95°C, and the water bath extraction time is 3–6 hours.

8. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 1, characterized in that, The preparation method of the compound tea product in step four includes: (1) Weigh out the following ingredients according to their mass percentages: tea leaves, rose petals, Imperata cylindrica root, Coix seed sprouts, Plantago asiatica seed, lily polysaccharide, chicory, Ligusticum chuanxiong, Angelica sinensis, licorice, dipotassium hydrogen phosphate, and sodium bicarbonate, and set aside for later use. (2) After crushing and sieving the Imperata cylindrica root, it was extracted by reflux with ethanol. The resulting extract was then extracted with petroleum ether, ethyl acetate and n-butanol in sequence. The extract was evaporated and dried to obtain Imperata cylindrica root extract. (3) Place rose petals, plantain seeds, chicory, Sichuan lovage, angelica and licorice in a vegetable washing machine, add 10% pesticide residue cleaning solution to water at 55-65℃ for cleaning, and then rinse with clean water 2-4 times. (4) The cleaned raw materials obtained in step (3) are baked in a hot air rotary oven at 60-70°C for 2-3 hours, and then pulverized into fine powder of 160 mesh for later use. (5) The rose pollen, plantain seed powder, chicory powder, chuanxiong powder, angelica powder and licorice powder obtained in step (4) are mixed evenly with the pretreated tea, Imperata cylindrica root extract, lily polysaccharide, Imperata cylindrica root, dipotassium hydrogen phosphate and sodium bicarbonate, sterilized and packaged in 10g / pack to obtain the compound tea product.

9. The method for preparing the tea product for relieving gout and regulating endocrine balance as described in claim 8, characterized in that, The preparation method of the Imperata cylindrica root extract in step (2) includes: 1) After crushing the Imperata cylindrica root and passing it through a 100-120 mesh sieve, extract it by reflux with a 50-90 wt% ethanol aqueous solution at 80-85℃ for 1-3 hours. The resulting extract is concentrated to obtain a paste. 2) After suspending the extract obtained in step 1) in water, extract it with petroleum ether, extract the aqueous phase obtained by extraction with ethyl acetate, and then extract the aqueous phase obtained by ethyl acetate extraction with n-butanol. 3) After evaporating and drying the raffinate obtained from the n-butanol extraction in step 2), the Imperata cylindrica root extract was obtained.

10. A tea product for relieving gout and regulating endocrine balance prepared using the method for preparing a tea product for relieving gout and regulating endocrine balance as described in any one of claims 1 to 9, characterized in that, The tea product for relieving gout and regulating endocrine balance is composed of 20-40 parts tea leaves, 20-30 parts rose petals, 10-20 parts Imperata cylindrica root, 10-15 parts Coix seed buds, 8-15 parts Plantago asiatica seed, 6-12 parts lily polysaccharide, 5-10 parts chicory, 4-8 parts Ligusticum chuanxiong, 3-7 parts Angelica sinensis, 2-4 parts licorice, 2-3 parts dipotassium hydrogen phosphate, and 1-2 parts sodium bicarbonate, by weight.

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