Meal replacement powder for improving intestinal environment and preparation method thereof

Abstract

The invention relates to the technical field of meal replacement powder, provides meal replacement powder for improving intestinal environment and a preparation method, and aims to provide comprehensive nutrition and promote intestinal health through raw material combination in scientific proportion. The meal replacement powder consists of a component A (hemp seed protein powder and quinoa protein isolate), a component B (semen plantaginis husk powder and cactus fruit powder), a component C (oenothera biennis oil powder and sea buckthorn seed oil powder), a component D (agave syrup powder) and a component E (nattokinase and bacillus coagulans viable powder). The preparation method comprises the steps of low-temperature superfine grinding, multi-enzyme composite enzymolysis, embedding of probiotics and enzymes, fat microencapsulation, mixing and grinding, drying and packaging and the like. The meal replacement powder capable of improving the intestinal environment has positive effects in the aspects of promoting small intestine peristalsis, improving constipation, increasing the number of beneficial bacteria, reducing the number of harmful bacteria and maintaining intestinal microecological balance.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of meal replacement powder, in particular to a meal replacement powder for improving intestinal environment and a preparation method thereof. BACKGROUND

[0002] Meal replacement powder is a kind of food aiming to replace a meal or part of a meal to provide comprehensive nutrition, control calorie intake and assist in weight management. It usually contains protein, dietary fiber, vitamins, minerals and other nutrients to meet the daily energy and nutritional needs of the human body. Meal replacement powder is characterized by convenience, speed, balanced nutrition, and is suitable for health seekers, weight loss people and patients with specific diseases in fast-paced life.

[0003] Most of the existing meal replacement powder products ignore the balance of overall nutrition due to the pursuit of calorie control or the supplementation of certain nutrients, and there are deficiencies in the content of dietary fiber, active ingredients such as probiotics and enzymes, and absorption utilization rate, which cannot meet the needs of improving intestinal health. SUMMARY

[0004] In order to solve the problems in the background art, the present application provides a meal replacement powder for improving intestinal environment, which is made of the following raw materials by weight:

[0005] A component: including hemp seed protein powder 30 parts, quinoa separated protein 20 parts;

[0006] B component: including psyllium husk powder 20 parts, cactus fruit powder 10 parts;

[0007] C component: including evening primrose oil powder 10 parts, sea buckthorn seed oil powder 5 parts;

[0008] D component: including agave syrup powder 5 parts;

[0009] E component: including natto kinase 1 part, bacillus coagulans live bacteria powder 4 parts.

[0010] The preparation method of the meal replacement powder for improving intestinal environment includes the following steps:

[0011] S1: low-temperature ultrafine grinding; the solid raw materials, including hemp seed protein powder, quinoa separated protein, psyllium husk powder and cactus fruit powder, are subjected to low-temperature ultrafine grinding, and the grinding particle size reaches 50-80 microns;

[0012] S2: multi-enzyme complex enzymolysis; proteinase and cellulase are added to the ground hemp seed protein powder and quinoa separated protein, and enzymolysis reaction is carried out at 40°C-50°C to degrade protein macromolecules;

[0013] S3: Probiotic and enzyme encapsulation; Nattokinase and Bacillus coagulans live bacteria powder are mixed with sodium alginate and gelatin as wall materials, and microcapsule encapsulation is performed using supercritical CO2 as a solvent;

[0014] S4: Fat microencapsulation; Evening primrose oil powder and sea buckthorn seed oil powder are emulsified separately, and then mixed with modified starch and gum arabic as wall materials; the emulsion is converted into microcapsule particles using spray drying technology;

[0015] S5: Mixing and pulverizing; The components that have undergone the above treatment are mixed evenly in a vacuum mixer;

[0016] S6: Drying and Packaging; The granulated meal replacement powder is placed in a low-temperature vacuum dryer for drying to remove excess moisture and prevent oxidation; After drying, it is sealed in light-proof and moisture-proof packaging materials.

[0017] Furthermore, the specific process of step S1 includes:

[0018] S11: Accurately weigh the hemp seed protein powder, quinoa protein isolate, psyllium husk powder, and cactus fruit powder according to the formula ratio, mix them evenly and place them in a sealed container for later use.

[0019] S12: A liquid nitrogen tank is used to provide a continuous and stable supply of liquid nitrogen, and the temperature of the pulverizing chamber is maintained at -160°C by real-time monitoring and adjustment through a temperature controller.

[0020] S13: Select an ultrafine pulverizer with a high-precision classifying wheel, and set the blade speed to 85,000 rpm; adjust the gap between the classifying wheels to the range of 0.05-0.2 micrometers to achieve a particle size of 50-200 nanometers;

[0021] S14: Slowly feed the uniformly mixed raw materials into the crushing chamber through the low-temperature feeding system, with the feeding rate controlled at 0.8g / min; start the crusher and simultaneously turn on the liquid nitrogen spraying device to ensure that it is always at a low temperature of -160°C during the crushing process;

[0022] S15: Collect the pulverized nano-powder and remove surface residues by purging with nitrogen; transfer the powder to a low-temperature vacuum dryer, setting the temperature to 35°C and maintaining a vacuum of 10. -3 Pa, dry for 2 hours.

[0023] Furthermore, the specific process of step S2 includes:

[0024] S21: Place the crushed hemp seed protein powder and quinoa protein isolate in a sterile mixing container and stir at low speed for 5 minutes using a mechanical stirrer.

[0025] S22: Prepare a compound enzyme solution by adding 0.5% w / w neutral protease with 50,000 U / g activity units and 0.3% w / w cellulase with 30,000 U / g activity units. Adjust the pH of the enzyme hydrolysate to 6.5 using a precision pH meter and preheat it to the enzymatic reaction temperature of 45°C using a constant temperature water bath.

[0026] S23: Slowly pour the well-mixed raw materials into the preheated enzymatic hydrolysate while stirring to ensure that the enzyme and substrate are in full contact. Set the enzymatic hydrolysis time to 4 hours. After the enzymatic hydrolysis time is reached, heat the enzymatic hydrolysate to 85°C and maintain it for 10 minutes to terminate the enzymatic hydrolysis reaction.

[0027] S24: Cool to room temperature and remove impurities and incompletely degraded macromolecules through a 0.22μm microporous membrane;

[0028] S25: Transfer the filtered enzymatic hydrolysate to a sterile storage container and seal it for storage at 4°C.

[0029] Furthermore, the specific process of step S3 includes:

[0030] S31: Core material preparation: Accurately weigh 20g of nattokinase and Bacillus coagulans live bacteria powder, with a live bacteria count ≥10. 9 CFU / g, 80g, mix well and place in a sterile container for later use;

[0031] S32: Wall material preparation, select sodium alginate 1.5% w / w and gelatin 1.0% w / w as wall material, dissolve in deionized water, use a magnetic stirrer at 60°C until completely dissolved, forming a transparent and homogeneous wall material solution;

[0032] S33: Core material-wall material mixing: Slowly add the core material to the preheated wall material solution while stirring with a high-speed disperser at 8,000 rpm to ensure that the core material is evenly dispersed in the wall material to form a stable emulsion.

[0033] S34: Supercritical CO2 microcapsule encapsulation. An emulsion is transferred to the reactor of a supercritical CO2 microcapsule encapsulation device. The reaction pressure is set to 30 MPa and the temperature to 35°C. Supercritical CO2 is introduced as a solvent. By adjusting the CO2 flow rate to 5 L / min and the reaction time to 4 hours, CO2 is allowed to fully penetrate and dissolve in the emulsion, promoting the deposition and solidification of the wall material to form microcapsules. Under high pressure and low temperature conditions, the supercritical state of CO2 facilitates rapid densification of the wall material, improving encapsulation efficiency and the mechanical strength of the wall material. After the reaction, CO2 is released by depressurization, causing the microcapsules to precipitate from the reactor. A freeze dryer is used, with the temperature set to -50°C and the vacuum degree to 10. -3Pa dries the microcapsules to remove residual moisture and CO2;

[0034] S35: The dried microcapsules are sieved through a vibrating screen to remove particles that are too large or too small, and then set aside for later use.

[0035] Furthermore, the specific process of step S4 includes:

[0036] S41: Emulsion preparation: Evening primrose oil powder and sea buckthorn seed oil powder are mixed with deionized water in a 1:2 ratio and stirred at 10,000 rpm for 10 minutes using a high-speed disperser to form a uniform oil-water mixture; 5% of the total oil content of the emulsifier Tween 80 is added to the oil-water mixture and high-speed shear emulsification is continued at 12,000 rpm for 30 minutes until a fine and uniform emulsion is formed;

[0037] S42: Select 20% modified starch and 10% gum arabic as the composite wall material, mix them evenly, and then slowly add them to the emulsion while stirring at a low speed of 500 rpm to avoid generating a lot of foam. The mixing time should be no less than 30 minutes to ensure that the wall material is fully adsorbed on the surface of the oil droplets.

[0038] S43: Start the spray dryer, set the inlet temperature to 180°C, the outlet temperature to 70°C, and the spray rate to 200 mL / min to ensure that the emulsion can be sprayed into the drying chamber evenly and continuously;

[0039] S44: Once the temperature in the drying chamber has stabilized, start the peristaltic pump to pump the emulsion into the nozzle of the spray dryer for atomization; adjust the nozzle pressure to 0.4 MPa to ensure that the droplets are small and uniform; the droplets quickly come into contact with the hot air in the drying chamber, the moisture evaporates rapidly, and the wall material solidifies to form microcapsule particles, which are then collected by a cyclone separator;

[0040] S45: Allow the collected microcapsule products to cool naturally to room temperature, then sieve them through a vibrating screen with an 80-mesh sieve to remove clumps and larger particles, and set aside for later use.

[0041] Furthermore, the specific process of step S5 includes:

[0042] S51: A vacuum mixer employing high vacuum and high-efficiency stirring, with the following parameter settings: Vacuum degree set to 10. -3 Pa; the stirring speed is set to 1000 rpm; the raw materials of each component after the previous treatment are added to the vacuum mixer in sequence, the stirring system is started, the set vacuum degree and stirring speed are continuously monitored and maintained, and the mixing time is set to 30 minutes;

[0043] S52: Use a sieve with an 800-micron aperture to perform preliminary screening of the mixed raw materials to remove larger particles or impurities;

[0044] S53: The raw materials after preliminary screening are sent to an ultra-fine pulverizer for fine grinding.

[0045] S54: Use a sieve with a 500-micron aperture to precisely sieve the finely ground meal replacement powder to ensure that the particle size of all particles does not exceed 500 microns.

[0046] The beneficial effects achieved by this invention are as follows:

[0047] First, this invention, through repeated formula optimization, designs a formula consisting of components A to E. By scientifically combining various high-quality raw materials, it provides comprehensive nutritional components, offering benefits such as improving gut health, promoting satiety, reducing glycemic response, providing healthy fats, anti-oxidation, thrombolysis, and improving gut microbiota. Specifically:

[0048] Component A provides a high-quality protein source; 30 servings of hemp seed protein powder + 20 servings of quinoa protein isolate: Both hemp seed protein powder and quinoa protein isolate are high-quality protein sources, rich in essential amino acids, which help enhance immunity and promote muscle growth and repair. Hemp seed protein powder not only contains high-quality plant protein but also Omega-3 and Omega-6 fatty acids, as well as various minerals and vitamins, providing comprehensive nutritional support. Quinoa protein isolate has complete protein characteristics, high digestibility and absorption, making it suitable as the main protein source for meal replacement powders. The amino acid composition of hemp seed protein powder and quinoa protein isolate is complementary, allowing them to compensate for each other's amino acid deficiencies and improve the bioavailability of the protein.

[0049] Component B consists of 20 parts psyllium husk powder and 10 parts cactus fruit powder. Both psyllium husk powder and cactus fruit powder are rich in soluble dietary fiber, which increases stool volume, softens stool, promotes intestinal peristalsis, and effectively prevents and improves constipation. Dietary fiber can also absorb harmful substances in the intestines, helping to maintain intestinal health. Dietary fiber absorbs water and swells, forming a larger volume in the stomach, thereby increasing satiety, reducing food intake, and helping to control weight. Soluble dietary fiber can slow down the digestion and absorption of food in the gastrointestinal tract, thereby reducing postprandial blood sugar fluctuations, which is especially beneficial for diabetic patients.

[0050] Component C provides healthy fats; 10 parts evening primrose oil powder + 5 parts sea buckthorn seed oil powder; evening primrose oil powder and sea buckthorn seed oil powder are rich in various unsaturated fatty acids, such as gamma-linolenic acid (GLA), Omega-3, and Omega-6, which have significant effects on cardiovascular health and possess anti-inflammatory and endocrine-regulating properties. Evening primrose oil powder and sea buckthorn seed oil powder also contain various antioxidant components, such as vitamin E, which can scavenge free radicals in the body, delay the aging process, and protect cells from oxidative damage.

[0051] Component D is a low-GI sweetener; 5 parts agave syrup powder: As a low-GI (glycemic index) sweetener, agave syrup powder can provide sweetness while avoiding a sharp rise in blood sugar, making it suitable for diabetics and people who need to control their weight.

[0052] Component E consists of 1 part nattokinase and 4 parts live Bacillus coagulans powder: Nattokinase has a strong thrombolytic effect, capable of dissolving blood clots, reducing blood viscosity, and preventing cardiovascular and cerebrovascular diseases. Bacillus coagulans is a probiotic that effectively regulates the balance of intestinal flora, promotes the growth of beneficial bacteria, inhibits the reproduction of harmful bacteria, enhances intestinal immunity, and improves intestinal function. This probiotic can secrete a variety of digestive enzymes, helping the body to better digest and absorb nutrients from food, improving nutrient utilization.

[0053] Secondly, in the formulation of this invention, components A to E work synergistically to promote each other and jointly exert multiple beneficial effects such as improving the intestinal environment, providing comprehensive nutrition, and enhancing immunity. Specifically, this is manifested in:

[0054] The high-quality protein in component A provides essential nutrients for probiotics in the gut (such as the Bacillus coagulans live bacteria powder in component E). Protein is an important energy source for the growth and reproduction of probiotics. The presence of component A helps probiotics in component E to colonize and proliferate in the gut, thereby better playing their role in regulating the gut microbiota and enhancing intestinal immunity.

[0055] The soluble dietary fiber in component B promotes intestinal peristalsis and improves constipation, which aids in the digestion and absorption of protein and other nutrients in component A. A healthy gut environment ensures the full absorption and utilization of nutrients.

[0056] The soluble dietary fiber in component B can increase the water content in the intestines, which helps the unsaturated fatty acids in evening primrose oil powder and sea buckthorn seed oil powder to dissolve and be absorbed in the intestines, thereby improving the utilization rate of these healthy fats.

[0057] The unsaturated fatty acids in component C have anti-inflammatory effects, which can reduce intestinal inflammation and create a more favorable living environment for probiotics (Bacillus coagulans live bacteria powder). At the same time, these healthy fats are also essential for probiotics to synthesize cell membranes and produce energy.

[0058] The Bacillus coagulans live bacteria powder in component E improves the intestinal environment by regulating the balance of intestinal flora, promoting the growth of beneficial bacteria, and inhibiting the proliferation of harmful bacteria. This healthy intestinal environment facilitates the digestion and absorption of protein in component A, improving its bioavailability; it also promotes the fermentation and utilization of soluble dietary fiber in component B, enhancing its effect on improving intestinal health, and further promotes the absorption and utilization of unsaturated fatty acids in component C.

[0059] Third, the preparation process of the meal replacement powder for improving the intestinal environment of the present invention utilizes a series of advanced technologies, including low-temperature ultrafine grinding, multi-enzyme complex enzymatic hydrolysis, probiotic and enzyme encapsulation, emulsion preparation, and spray drying, to achieve efficient extraction, protection, dispersion, and stabilization of nutritional components, as detailed below:

[0060] By using low-temperature ultrafine grinding technology, solid raw materials are pulverized to the micron level, which significantly increases the specific surface area of ​​the raw materials and improves the dissolution rate and bioavailability of nutrients.

[0061] The use of multi-enzyme (protease and cellulase) combined enzymatic hydrolysis technology can effectively degrade large protein and polysaccharide molecules, reduce their molecular weight, and improve their digestibility and absorption in the body. Simultaneously, enzymatic hydrolysis also releases bioactive small peptides, further enhancing the product's nutritional value and health benefits.

[0062] By using supercritical CO2 as a solvent for microencapsulation technology, probiotics (Bacillus coagulans live bacteria powder) and enzymes (nattokinase) are encapsulated in a wall material (sodium alginate and gelatin), effectively protecting the activity of the probiotics and enzymes and improving their stability during storage and processing. This also facilitates uniform dispersion and release in meal replacement powders.

[0063] The oil phase (evening primrose oil powder and sea buckthorn seed oil powder) is uniformly dispersed in the aqueous phase using a high-speed disperser and spray dryer to form a stable emulsion, which is then spray-dried into a powdered meal replacement powder. This process not only ensures the uniform dispersion and stability of the oil phase components but also improves the product's solubility and reconstitution properties. Attached Figure Description

[0064] Figure 1 This is a flowchart of a method for preparing a meal replacement powder that improves the intestinal environment according to the present invention. Detailed Implementation

[0065] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. In addition, the forms of the various structures described in the following embodiments are merely illustrative. The present invention is not limited to the structures described in the following embodiments. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0066] The components of this invention are as follows:

[0067] Component A: Primarily contains protein. Hemp seeds are not only rich in high-quality plant protein, but also contain Omega-3 and Omega-6 fatty acids, as well as various minerals and vitamins, making them a nutrient-rich protein source. Quinoa protein isolate: Its complete protein properties make it ideal as a protein source.

[0068] Component B: Primarily contains vitamins and dietary fiber. Psyllium husk powder is rich in soluble dietary fiber, which helps improve gut health and promotes intestinal motility. It also contains various vitamins and minerals, making it a relatively uncommon but nutrient-rich source of dietary fiber. Cactus fruit powder: Not only is it rich in vitamin C and various minerals, but it also contains a certain amount of dietary fiber; it is relatively rare as a food ingredient.

[0069] Component C: Primarily contains healthy fats. Evening primrose oil powder: Rich in gamma-linolenic acid (GLA), an Omega-6 fatty acid beneficial to human health, with anti-inflammatory and endocrine-regulating effects, and is a healthy source of fat.

[0070] Sea buckthorn seed oil powder: It is rich in unsaturated fatty acids, vitamin E and a variety of bioactive substances, and has a variety of health benefits such as anti-oxidation and anti-inflammation. It is also a relatively uncommon source of healthy fats.

[0071] Component D: Primarily contains sugar. Unlike traditional sucrose, agave syrup powder has a lower glycemic index (GI) and is rich in minerals and antioxidants, making it a niche but healthy sugar alternative.

[0072] Component E: Primarily contains enzymes and probiotics. Nattokinase is an enzyme with thrombolytic activity. Bacillus coagulans is a probiotic that offers various benefits for improving the gut environment.

[0073] The following is a description of some of the terminology used in this invention:

[0074] Tween 80, also known as polysorbate 80 or polyoxyethylene sorbitan monooleate, is an important nonionic surfactant. Tween 80 is obtained by esterification of sorbitan and oleic acid, followed by polymerization with ethylene oxide. Its molecule contains both hydrophilic polyoxyethylene segments and lipophilic oleate segments; this unique amphiphilic structure endows Tween 80 with excellent emulsifying properties.

[0075] The supercritical CO2 microcapsule encapsulation device is a specialized instrument for supercritical CO2 microcapsule encapsulation technology. Utilizing the unique physical properties of supercritical CO2, active components are effectively encapsulated within the wall material to form microcapsules.

[0076] The supercritical CO2 microcapsule encapsulation device is equipped with a high-pressure reactor to contain the core material, wall material, and supercritical CO2. The reactor can withstand the high-pressure environment, ensuring the stable presence of supercritical CO2. The CO2 supply system includes a CO2 storage tank, compressor, and flow control valves to provide a stable flow rate of supercritical CO2. The heating and cooling system precisely controls the temperature within the reactor to meet the requirements of the supercritical CO2 state. The stirring system ensures uniform mixing of the core and wall materials in the supercritical CO2, promoting the deposition and solidification of the wall material. The collection and separation system collects the microcapsules after the reaction and removes residual CO2 and solvent.

[0077] The supercritical CO2 microencapsulation device operates by mixing the core material and wall material in a specific ratio, then introducing supercritical CO2 into a high-pressure reactor via a CO2 supply system, adjusting the pressure and temperature to the set parameters. Microcapsule formation occurs under the influence of supercritical CO2, where the wall material gradually dissolves and deposits on the surface of the core material, forming microcapsules. A stirring system ensures uniform mixing of the core and wall materials, promoting microcapsule formation. After the reaction, residual CO2 and solvent are removed via a collection and separation system, yielding the microcapsule product. Further post-processing steps, such as drying and sieving, may be required.

[0078] Example 1: In this example, a meal replacement powder for improving the intestinal environment is made from the following ingredients in parts by weight:

[0079] Component A: Includes 30g of hemp seed protein powder and 20g of quinoa protein isolate;

[0080] Component B: includes 20 grams of psyllium husk powder and 10 grams of cactus fruit powder;

[0081] Component C: includes 10 grams of evening primrose oil powder and 5 grams of sea buckthorn seed oil powder;

[0082] Component D: Includes 5 grams of agave syrup powder;

[0083] Component E: includes 1 gram of nattokinase and 4 grams of live Bacillus coagulans powder.

[0084] Example 2: In this example, the raw materials from Example 1 are used, and the following steps are followed:

[0085] S1: Low-temperature ultrafine grinding; solid raw materials, including hemp seed protein powder, quinoa protein isolate, psyllium husk powder, and cactus fruit powder, are subjected to low-temperature ultrafine grinding to achieve a particle size of 50-80 micrometers.

[0086] S2: Multi-enzyme complex hydrolysis; Protease and cellulase are added to the pulverized hemp seed protein powder and quinoa protein isolate, and the enzymatic hydrolysis reaction is carried out at 40°C-50°C to degrade the protein macromolecules;

[0087] S3: Probiotic and enzyme encapsulation; Nattokinase and Bacillus coagulans live bacteria powder are mixed with sodium alginate and gelatin as wall materials, and microcapsule encapsulation is performed using supercritical CO2 as a solvent;

[0088] S4: Fat microencapsulation; Evening primrose oil powder and sea buckthorn seed oil powder are emulsified separately, and then mixed with modified starch and gum arabic as wall materials; the emulsion is converted into microcapsule particles using spray drying technology;

[0089] S5: Mixing and pulverizing; The components that have undergone the above treatment are mixed evenly in a vacuum mixer;

[0090] S6: Drying and Packaging; The granulated meal replacement powder is placed in a low-temperature vacuum dryer for drying to remove excess moisture and prevent oxidation; After drying, it is sealed in light-proof and moisture-proof packaging materials.

[0091] The specific process of step S1 includes:

[0092] S11: Accurately weigh the hemp seed protein powder, quinoa protein isolate, psyllium husk powder, and cactus fruit powder according to the formula ratio, mix them evenly and place them in a sealed container for later use.

[0093] S12: A liquid nitrogen tank is used to provide a continuous and stable supply of liquid nitrogen, and the temperature of the pulverizing chamber is maintained at -160°C by real-time monitoring and adjustment through a temperature controller.

[0094] S13: Select an ultrafine pulverizer with a high-precision classifying wheel, and set the blade speed to 85,000 rpm; adjust the gap between the classifying wheels to the range of 0.05-0.2 micrometers to achieve a particle size of 50-200 nanometers;

[0095] S14: Slowly feed the uniformly mixed raw materials into the crushing chamber through the low-temperature feeding system, with the feeding rate controlled at 0.8g / min; start the crusher and simultaneously turn on the liquid nitrogen spraying device to ensure that it is always at a low temperature of -160°C during the crushing process;

[0096] S15: Collect the pulverized nano-powder and remove surface residues by purging with nitrogen; transfer the powder to a low-temperature vacuum dryer, setting the temperature to 35°C and maintaining a vacuum of 10. -3 Pa, dry for 2 hours.

[0097] The specific process of step S2 includes:

[0098] S21: Place the crushed hemp seed protein powder and quinoa protein isolate in a sterile mixing container and stir at low speed for 5 minutes using a mechanical stirrer.

[0099] S22: Prepare a compound enzyme solution by adding 0.5% w / w neutral protease with 50,000 U / g activity units and 0.3% w / w cellulase with 30,000 U / g activity units. Adjust the pH of the enzyme hydrolysate to 6.5 using a precision pH meter and preheat it to the enzymatic reaction temperature of 45°C using a constant temperature water bath.

[0100] S23: Slowly pour the well-mixed raw materials into the preheated enzymatic hydrolysate while stirring to ensure that the enzyme and substrate are in full contact. Set the enzymatic hydrolysis time to 4 hours. After the enzymatic hydrolysis time is reached, heat the enzymatic hydrolysate to 85°C and maintain it for 10 minutes to terminate the enzymatic hydrolysis reaction.

[0101] S24: Cool to room temperature and remove impurities and incompletely degraded macromolecules through a 0.22μm microporous membrane;

[0102] S25: Transfer the filtered enzymatic hydrolysate to a sterile storage container and seal it for storage at 4°C.

[0103] The specific process of step S3 includes:

[0104] S31: Core material preparation: Accurately weigh 20g of nattokinase and Bacillus coagulans live bacteria powder, with a live bacteria count ≥10. 9 CFU / g, 80g, mix well and place in a sterile container for later use;

[0105] S32: Wall material preparation, select sodium alginate 1.5% w / w and gelatin 1.0% w / w as wall material, dissolve in deionized water, use a magnetic stirrer at 60°C until completely dissolved, forming a transparent and homogeneous wall material solution;

[0106] S33: Core material-wall material mixing: Slowly add the core material to the preheated wall material solution while stirring with a high-speed disperser at 8,000 rpm to ensure that the core material is evenly dispersed in the wall material to form a stable emulsion.

[0107] S34: Supercritical CO2 microcapsule encapsulation. An emulsion is transferred to the reactor of a supercritical CO2 microcapsule encapsulation device. The reaction pressure is set to 30 MPa and the temperature to 35°C. Supercritical CO2 is introduced as a solvent. By adjusting the CO2 flow rate to 5 L / min and the reaction time to 4 hours, CO2 is allowed to fully penetrate and dissolve in the emulsion, promoting the deposition and solidification of the wall material to form microcapsules. Under high pressure and low temperature conditions, the supercritical state of CO2 facilitates rapid densification of the wall material, improving encapsulation efficiency and the mechanical strength of the wall material. After the reaction, CO2 is released by depressurization, causing the microcapsules to precipitate from the reactor. A freeze dryer is used, with the temperature set to -50°C and the vacuum degree to 10. -3 Pa dries the microcapsules to remove residual moisture and CO2;

[0108] S35: The dried microcapsules are sieved through a vibrating screen to remove particles that are too large or too small, and then set aside for later use.

[0109] The specific process of step S4 includes:

[0110] S41: Emulsion preparation: Evening primrose oil powder and sea buckthorn seed oil powder are mixed with deionized water in a 1:2 ratio and stirred at 10,000 rpm for 10 minutes using a high-speed disperser to form a uniform oil-water mixture; 5% of the total oil content of the emulsifier Tween 80 is added to the oil-water mixture and high-speed shear emulsification is continued at 12,000 rpm for 30 minutes until a fine and uniform emulsion is formed;

[0111] S42: Select 20% modified starch and 10% gum arabic as the composite wall material, mix them evenly, and then slowly add them to the emulsion while stirring at a low speed of 500 rpm to avoid generating a lot of foam. The mixing time should be no less than 30 minutes to ensure that the wall material is fully adsorbed on the surface of the oil droplets.

[0112] S43: Start the spray dryer, set the inlet temperature to 180°C, the outlet temperature to 70°C, and the spray rate to 200 mL / min to ensure that the emulsion can be sprayed into the drying chamber evenly and continuously;

[0113] S44: Once the temperature in the drying chamber has stabilized, start the peristaltic pump to pump the emulsion into the nozzle of the spray dryer for atomization; adjust the nozzle pressure to 0.4 MPa to ensure that the droplets are small and uniform; the droplets quickly come into contact with the hot air in the drying chamber, the moisture evaporates rapidly, and the wall material solidifies to form microcapsule particles, which are then collected by a cyclone separator;

[0114] S45: Allow the collected microcapsule products to cool naturally to room temperature, then sieve them through a vibrating screen with an 80-mesh sieve to remove clumps and larger particles, and set aside for later use.

[0115] The specific process of step S5 includes:

[0116] S51: A vacuum mixer employing high vacuum and high-efficiency stirring, with the following parameter settings: Vacuum degree set to 10. -3 Pa; the stirring speed is set to 1000 rpm; the raw materials of each component after the previous treatment are added to the vacuum mixer in sequence, the stirring system is started, the set vacuum degree and stirring speed are continuously monitored and maintained, and the mixing time is set to 30 minutes;

[0117] S52: Use a sieve with an 800-micron aperture to perform preliminary screening of the mixed raw materials to remove larger particles or impurities;

[0118] S53: The raw materials after preliminary screening are sent to an ultra-fine pulverizer for fine grinding.

[0119] S54: Use a sieve with a 500-micron aperture to precisely sieve the finely ground meal replacement powder to ensure that the particle size of all particles does not exceed 500 microns.

[0120] Experiment Example 1: The specific experimental conditions for this experiment are as follows:

[0121] Experimental animals: 100 male Kunming mice, SPF grade, weighing 18-22g.

[0122] Experimental group feed: Meal replacement powder for improving the intestinal environment prepared according to the formula in Example 1 and the preparation method in Example 2.

[0123] Control group diet: standard mouse diet.

[0124] Reagents and equipment: Loperamide hydrochloride (for constructing a constipation model), precision electronic balance, high-speed multi-functional grinder, ultrafine grinding and vibration mill, constant temperature water bath, electric drying oven, microbial culture equipment (for intestinal flora analysis).

[0125] Adaptive feeding: 100 mice were acclimatized and fed in the experimental environment for 5 days.

[0126] Grouping: Mice were randomly divided into 5 groups, with 20 mice in each group.

[0127] Blank control group: This group of mice did not receive any special treatment and maintained a normal breeding environment. It served as the baseline group for the experiment and was used to compare with other treatment groups to evaluate the effect of the experimental treatment.

[0128] Model control group: This group of mice was given a constipation model via gavage with loperamide hydrochloride, but did not receive the intervention of the meal replacement powder to improve the intestinal environment. This group was used to assess whether the constipation model was successfully established and served as a reference for the treatment group to observe the effect of the meal replacement powder on improving constipation.

[0129] Low-dose group: After establishing a constipation model, this group of mice received a low-dose (equivalent to 2.5 times the recommended human dose) of a meal replacement powder to improve the intestinal environment. By observing the response of this group of mice, the effect of the low-dose meal replacement powder on improving constipation can be evaluated.

[0130] Medium-dose group: This group of mice, after establishing a constipation model, received a medium dose (equivalent to 5 times the recommended human dose) of meal replacement powder to improve the intestinal environment. This group was used to further evaluate the differences in the effects of the meal replacement powder at different doses.

[0131] High-dose group: After establishing a constipation model, this group of mice received a high dose (equivalent to 10 times the recommended human dose) of a meal replacement powder to improve the intestinal environment. By observing the response of this group of mice, we can assess whether the high-dose meal replacement powder is more effective than the low- and medium-dose groups in improving constipation.

[0132] The experimental method is as follows:

[0133] Constipation model construction:

[0134] Except for the blank control group, mice in the other groups were administered loperamide hydrochloride by gavage to establish a constipation model for a certain period of time.

[0135] Meal replacement powder intervention:

[0136] Each intervention group was given the corresponding meal replacement powder or standard feed by gavage according to the set dose for a certain period of time (e.g., 14 days).

[0137] Indicator Testing:

[0138] Small intestinal motility experiment: assessing small intestinal peristalsis by measuring the rate of ink propulsion in the small intestine.

[0139] Defecation experiment: Record the time of the first black stool, the number of stools, weight, and water content within 6 hours.

[0140] Gut microbiota analysis: Fecal samples were collected from mice to analyze the gut microbiota and assess changes in the number of beneficial and harmful bacteria.

[0141] Data collection: Record various indicators of mice in each group.

[0142] Statistical analysis: Data analysis was performed using statistical software (such as Graphpad Prism 8). One-way ANOVA was used for comparisons between groups, and Tukey's HSD test was used for multiple comparisons.

[0143] The following is a set of specific experimental data tables obtained from statistical analysis results, verifying the laxative effect of the meal replacement powder for improving the intestinal environment of the present invention and its impact on the intestinal flora.

[0144] Table 1: Small Intestinal Motility Experiment Data

[0145] As shown in Table 1, compared with the model control group, the small intestinal ink propulsion rate of mice in the medium-dose and high-dose groups was significantly improved. This indicates that the meal replacement powder can effectively promote small intestinal peristalsis and improve intestinal motility at medium and high doses. Although the low-dose group also showed some improvement, the effect was not as significant as that of the medium- and high-dose groups.

[0146] Table 2: Defecation Experiment Data

[0147] Table 2 shows that the time to first black stool was significantly shortened in the medium and high dose groups, indicating that the meal replacement powder can accelerate defecation. The number and weight of stool particles increased within 6 hours in both the medium and high dose groups, indicating that the meal replacement powder helps increase stool volume. The increased water content in the feces of the medium and high dose groups indicates that the meal replacement powder helps soften the feces, making them easier to pass.

[0148] Table 3: Gut microbiota analysis data

[0149] As shown in Table 3, the number of Bifidobacteria and Lactobacilli in the intestines of mice in the medium and high dose groups increased significantly. These are beneficial bacteria that have a positive effect on intestinal health. At the same time, the number of harmful bacteria such as Enterococcus and Escherichia coli in mice in the medium and high dose groups decreased, indicating that the meal replacement powder helps to inhibit the growth of harmful bacteria.

[0150] This experimental example demonstrates that the meal replacement powder of the present invention, which improves the intestinal environment, effectively promotes small intestinal peristalsis, especially at medium and high doses. The meal replacement powder can shorten defecation time, increase stool volume and weight, and improve stool water content, thereby alleviating constipation. The meal replacement powder can increase the number of beneficial bacteria and reduce the number of harmful bacteria, helping to improve the intestinal flora structure and maintain the intestinal microecological environment. Its mechanism is as follows:

[0151] Water-holding capacity and swelling capacity of dietary fiber: Component B, containing psyllium husk powder and cactus fruit powder, is rich in soluble dietary fiber, which contains many hydrophilic groups. These groups can bind tightly to water molecules, forming hydrogen bonds, thereby attracting and retaining a large amount of water. This strong water-holding capacity allows dietary fiber to absorb and retain a large amount of water in the intestines, increasing the water content within the intestines.

[0152] When these dietary fibers enter the intestines, their strong water-holding capacity allows the stool to absorb and retain enough moisture, thus keeping the stool soft and easy to pass, avoiding constipation caused by overly dry stool.

[0153] Dietary fiber expands significantly in volume when it comes into contact with water, thus increasing the volume of chyme. This increase in volume not only makes people feel fuller after eating and reduces food intake, but also helps to mechanically stimulate the intestinal wall.

[0154] Promotes intestinal peristalsis: The increased volume of chyme and the increased weight of intestinal contents put greater pressure on the intestinal wall. This mechanical stimulation accelerates intestinal peristalsis, thereby promoting defecation.

[0155] Lubricating the intestinal wall and softening stool: Dietary fiber can form a lubricating film in the intestine, reducing friction between feces and the intestinal wall, making it easier and smoother to pass stool.

[0156] Because dietary fiber can absorb and retain a lot of water, it increases the water content of feces, thereby softening the stool, reducing resistance during defecation, and making the defecation process smoother.

[0157] Adjusting the gut microbiota structure: Dietary fiber can serve as a food source for beneficial bacteria in the gut (such as Bifidobacteria and Lactobacilli), especially probiotics like Bacillus coagulans (component E), promoting the growth and reproduction of these beneficial bacteria. An increase in the number of beneficial bacteria helps maintain gut health and enhances gut immunity.

[0158] Dietary fiber can absorb harmful substances in the intestines, reducing their bioavailability and thus inhibiting the growth and reproduction of harmful bacteria (such as enterococci and Escherichia coli). This inhibitory effect helps improve the gut microbiota structure and reduce the threat of harmful bacteria to gut health.

[0159] Through the water-holding capacity, swelling capacity, lubrication, and stool-softening effects of dietary fiber, the meal replacement powder of this invention significantly improves constipation and makes defecation smoother. By adjusting the intestinal flora structure, increasing the number of beneficial bacteria, and inhibiting the growth of harmful bacteria, the meal replacement powder of this invention effectively improves the intestinal microecological environment, enhances gastrointestinal motility, and improves overall health.

[0160] The meal replacement powder in this invention achieves multiple beneficial effects, such as promoting bowel movements, improving the intestinal microecological environment, and preventing constipation, by scientifically proportioning components A to E, especially component B which is rich in dietary fiber, and utilizing the water-holding and swelling power, lubrication and softening effect of dietary fiber, as well as its ability to adjust the intestinal flora structure.

[0161] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A meal replacement powder for improving the intestinal environment, characterized in that, It is made from the following raw materials in parts by weight: Component A: consists of 30 parts hemp seed protein powder and 20 parts quinoa protein isolate; Component B: consists of 20 parts psyllium husk powder and 10 parts cactus fruit powder; Component C: includes 10 parts evening primrose oil powder and 5 parts sea buckthorn seed oil powder; Component D: Includes 5 parts of agave syrup powder; Component E: includes 1 part nattokinase and 4 parts live Bacillus coagulans powder.

2. The method for preparing meal replacement powder for improving the intestinal environment as described in claim 1, characterized in that: It includes the following steps: S1: Low-temperature ultrafine grinding; solid raw materials, including hemp seed protein powder, quinoa protein isolate, psyllium husk powder, and cactus fruit powder, are subjected to low-temperature ultrafine grinding to achieve a particle size of 50-80 micrometers. S2: Multi-enzyme complex hydrolysis; Protease and cellulase are added to the pulverized hemp seed protein powder and quinoa protein isolate, and the enzymatic hydrolysis reaction is carried out at 40°C-50°C to degrade the protein macromolecules; S3: Probiotic and enzyme encapsulation; Nattokinase and Bacillus coagulans live bacteria powder are mixed with sodium alginate and gelatin as wall materials, and microcapsule encapsulation is performed using supercritical CO2 as a solvent; S4: Fat microencapsulation; Evening primrose oil powder and sea buckthorn seed oil powder are emulsified separately, and then mixed with modified starch and gum arabic as wall materials; the emulsion is converted into microcapsule particles using spray drying technology; S5: Mixing and pulverizing; The components that have undergone the above treatment are uniformly mixed in a vacuum mixer and then pulverized in an ultra-fine pulverizer; S6: Drying and Packaging; The granulated meal replacement powder is placed in a low-temperature vacuum dryer for drying to remove excess moisture and prevent oxidation; After drying, it is sealed in light-proof and moisture-proof packaging materials.

3. The meal replacement powder for improving the intestinal environment according to claim 1, characterized in that, The specific process of step S1 includes: S11: Accurately weigh the hemp seed protein powder, quinoa protein isolate, psyllium husk powder, and cactus fruit powder according to the formula ratio, mix them evenly and place them in a sealed container for later use. S12: A liquid nitrogen tank is used to provide a continuous and stable supply of liquid nitrogen, and the temperature of the pulverizing chamber is maintained at -160°C by real-time monitoring and adjustment through a temperature controller. S13: Select an ultrafine pulverizer with a high-precision classifying wheel, and set the blade speed to 85,000 rpm; adjust the gap between the classifying wheels to the range of 0.05-0.2 micrometers to achieve a particle size of 50-200 nanometers; S14: Slowly feed the uniformly mixed raw materials into the crushing chamber through the low-temperature feeding system, with the feeding rate controlled at 0.8g / min; start the crusher and simultaneously turn on the liquid nitrogen spraying device to ensure that it is always at a low temperature of -160°C during the crushing process; S15: Collect the pulverized nano-powder and remove surface residues by purging with nitrogen; transfer the powder to a low-temperature vacuum dryer, setting the temperature to 35°C and maintaining a vacuum of 10. -3 Pa, dry for 2 hours.

4. The meal replacement powder for improving the intestinal environment according to claim 1, characterized in that, The specific process of step S2 includes: S21: Place the crushed hemp seed protein powder and quinoa protein isolate in a sterile mixing container and stir at low speed for 5 minutes using a mechanical stirrer. S22: Prepare a compound enzyme solution by adding 0.5% w / w neutral protease with 50,000 U / g activity units and 0.3% w / w cellulase with 30,000 U / g activity units. Adjust the pH of the enzyme hydrolysate to 6.5 using a precision pH meter and preheat it to the enzymatic reaction temperature of 45°C using a constant temperature water bath. S23: Slowly pour the well-mixed raw materials into the preheated enzymatic hydrolysate while stirring to ensure that the enzyme and substrate are in full contact. Set the enzymatic hydrolysis time to 4 hours. After the enzymatic hydrolysis time is reached, heat the enzymatic hydrolysate to 85°C and maintain it for 10 minutes to terminate the enzymatic hydrolysis reaction. S24: Cool to room temperature and remove impurities and incompletely degraded macromolecules through a 0.22μm microporous membrane; S25: Transfer the filtered enzymatic hydrolysate to a sterile storage container and seal it for storage at 4°C.

5. The meal replacement powder for improving the intestinal environment according to claim 1, characterized in that, The specific process of step S3 includes: S31: Core material preparation: Accurately weigh 20g of nattokinase and Bacillus coagulans live bacteria powder, with a live bacteria count ≥10. 9 CFU / g, 80g, mix well and place in a sterile container for later use; S32: Wall material preparation, select sodium alginate 1.5% w / w and gelatin 1.0% w / w as wall material, dissolve in deionized water, use a magnetic stirrer at 60°C until completely dissolved, forming a transparent and homogeneous wall material solution; S33: Core material-wall material mixing: Slowly add the core material to the preheated wall material solution while stirring with a high-speed disperser at 8,000 rpm to ensure that the core material is evenly dispersed in the wall material to form a stable emulsion. S34: Supercritical CO2 microcapsule encapsulation. An emulsion is transferred to the reactor of a supercritical CO2 microcapsule encapsulation device. The reaction pressure is set to 30 MPa and the temperature to 35°C. Supercritical CO2 is introduced as a solvent. By adjusting the CO2 flow rate to 5 L / min and the reaction time to 4 hours, CO2 is allowed to fully penetrate and dissolve in the emulsion, promoting the deposition and solidification of the wall material to form microcapsules. Under high pressure and low temperature conditions, the supercritical state of CO2 facilitates rapid densification of the wall material, improving encapsulation efficiency and the mechanical strength of the wall material. After the reaction, CO2 is released by depressurization, causing the microcapsules to precipitate from the reactor. A freeze dryer is used, with the temperature set to -50°C and the vacuum degree to 10. -3 Pa dries the microcapsules to remove residual moisture and CO2; S35: The dried microcapsules are sieved through a vibrating screen to remove particles that are too large or too small, and then set aside for later use.

6. The meal replacement powder for improving the intestinal environment according to claim 1, characterized in that, The specific process of step S4 includes: S41: Emulsion preparation: Evening primrose oil powder and sea buckthorn seed oil powder are mixed with deionized water in a 1:2 ratio and stirred at 10,000 rpm for 10 minutes using a high-speed disperser to form a uniform oil-water mixture; 5% of the total oil content of the emulsifier Tween 80 is added to the oil-water mixture and high-speed shear emulsification is continued at 12,000 rpm for 30 minutes until a fine and uniform emulsion is formed; S42: Select 20% modified starch and 10% gum arabic as the composite wall material, mix them evenly, and then slowly add them to the emulsion while stirring at a low speed of 500 rpm to avoid generating a lot of foam. The mixing time should be no less than 30 minutes to ensure that the wall material is fully adsorbed on the surface of the oil droplets. S43: Start the spray dryer, set the inlet temperature to 180°C, the outlet temperature to 70°C, and the spray rate to 200mL / min to ensure that the emulsion can be sprayed into the drying chamber evenly and continuously; S44: Once the temperature in the drying chamber has stabilized, start the peristaltic pump to pump the emulsion into the nozzle of the spray dryer for atomization; adjust the nozzle pressure to 0.4 MPa to ensure that the droplets are small and uniform; the droplets quickly come into contact with the hot air in the drying chamber, the moisture evaporates rapidly, and the wall material solidifies to form microcapsule particles, which are then collected by a cyclone separator; S45: Allow the collected microcapsule products to cool naturally to room temperature, then sieve them through a vibrating screen with an 80-mesh sieve to remove clumps and larger particles, and set aside for later use.

7. The meal replacement powder for improving the intestinal environment according to claim 1, characterized in that, The specific process of step S5 includes: S51: A vacuum mixer employing high vacuum and high-efficiency stirring, with the following parameter settings: Vacuum degree set to 10. -3 Pa; the stirring speed is set to 1000 rpm; the raw materials of each component after the previous treatment are added to the vacuum mixer in sequence, the stirring system is started, the set vacuum degree and stirring speed are continuously monitored and maintained, and the mixing time is set to 30 minutes; S52: Use a sieve with an 800-micron aperture to perform preliminary screening of the mixed raw materials to remove larger particles or impurities; S53: The raw materials after preliminary screening are sent to an ultra-fine pulverizer for fine grinding. S54: Use a sieve with a 500-micron aperture to precisely sieve the finely ground meal replacement powder to ensure that the particle size of all particles does not exceed 500 microns.

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