A screening culture device for phosphorus-accumulating bacteria used in biological phosphorus removal of sewage

Abstract

This invention relates to a screening and cultivation device for phosphorus-uptake bacteria used in biological phosphorus removal from wastewater. The device includes a screening and cultivation chamber containing a screening and cultivation rack. The rack comprises a longitudinally oriented "U"-shaped base. A rectangular base plate is fixed to the lower end of the base, and a central support column is fixed to the center of the base plate. The central support column passes through the base and houses a rotating seat and a circular support plate. The support plate is fixed to the upper surface of the rotating seat, which is connected to the central support column via bearings. The support plate around the rotating seat has several circular perforations. Several annularly distributed limiting rods are fixed to the support plate around the perforations. A circular top plate is fixed to the upper end of each limiting rod, and the outer ring of the top plate has several feed holes corresponding to the perforations. The top of the rack within this screening and cultivation device features an automatic screening structure that can sort and arrange the culture dishes, facilitating the removal of designated culture dishes by a robotic arm.

Description

Technical Field

[0001] This invention relates to the technical field of screening incubators, and more specifically to a screening and cultivation device for phosphorus-absorbing bacteria strains used in biological phosphorus removal from wastewater. Background Technology

[0002] Biological strain screening incubators are key equipment in microbial research and industrial production. Their core function is to provide precise environmental control for the cultivation, screening, and functional verification of bacterial strains. In actual bacterial culture, the strains are placed in petri dishes, which are then placed in the biological strain screening incubator. Currently, some incubators have racks for storing empty petri dishes and racks for storing strained petri dishes. These racks are rotating supports with multiple rotating storage positions, each capable of holding multiple stacked petri dishes. Different culture conditions can be set in each petri dish to cultivate the same type of bacterial community, such as phosphorus-absorbing bacteria used for biological phosphorus removal in wastewater. Finally, testing can determine the optimal culture basis for the bacterial community. However, testing requires selecting a specific petri dish, which is placed among stacked petri dishes. This necessitates removing all the petri dishes above the designated one before retrieving the specific one, which is cumbersome. Therefore, an incubator with an built-in rack that enables automatic screening is needed. Summary of the Invention

[0003] The purpose of this invention is to address the shortcomings of existing technologies by providing a screening and cultivation device for phosphorus-absorbing bacteria strains used in biological phosphorus removal from wastewater. The device has an automatic screening structure on the top of the built-in rack, which can screen and organize the culture dishes, making it convenient for a robotic arm to remove the specified culture dishes.

[0004] A screening and cultivation device for phosphorus-absorbing bacteria for biological phosphorus removal in wastewater includes a screening and cultivation chamber with a screening and cultivation rack inside. The screening and cultivation rack includes a longitudinally shaped "U" base. A rectangular base plate is fixed to the lower end of the base, and a central support column is fixed to the middle of the base plate. The central support column passes through the base and is fitted with a rotating seat and a circular support plate. The support plate is fixed to the upper surface of the rotating seat, and the rotating seat is connected to the central support column via a bearing. Several circular perforations are formed on the support plate around the rotating seat. Several annularly distributed limiting rods are fixed to the support plate around the perforations. A circular top plate is fixed to the upper end of the limiting rods, and several feed holes are formed on the outer ring of the top plate, which are directly opposite the perforations.

[0005] A circular positioning plate abuts against the upper surface of the top plate. The upper end of the central support column extends out of the top plate and is inserted and fixed to the positioning plate. A through hole is formed on the rear side of the positioning plate, which is opposite to the feed hole on the top plate. Several annular screening frames are abutted against the upper surface of the outer ring of the positioning plate and are opposite to the through hole. Spokes are fixedly connected between adjacent screening frames. The inner end of the spokes is fixedly connected to a T-shaped rotary seat. A rotary motor is fixedly connected to the upper surface of the rotary seat. The shaft of the rotary motor is inserted and fixed to the central support column. The rotary seat is connected to the upper end of the central support column through a bearing.

[0006] A lifting plate is inserted into the through hole of the positioning plate. A limiting prism is fixedly attached to the lower end face of the lifting plate. A vertical threaded hole is formed in the limiting prism. The lower end of the limiting prism passes through the feed hole of the top plate and the through hole of the supporting bottom plate and is inserted into the base. A protective sleeve opposite to the limiting prism is fixedly attached to the lower end face of the rear side of the bottom plate. A lead screw is inserted into the protective sleeve. The upper end of the lead screw is screwed into the threaded hole of the limiting prism, and the lower end is fixedly connected to the shaft of the lifting motor through a coupling. The lifting motor is fixedly connected to the bottom of the protective sleeve.

[0007] Preferably, a gear ring is fixedly connected to the lower end of the rotating seat, and a gear meshes with the front side of the gear ring; a rotary motor is fixedly connected to the base, and the rotating shaft of the rotary motor extends out of the base and is inserted and fixed in the gear.

[0008] The lead screw and protective sleeve are both located on the rear side of the gear ring, and the base plate has clearance holes that are opposite to the lead screw and the limiting prism.

[0009] Preferably, the central axis of the central support column, the central axis of the rotating seat, the central axis of the supporting base plate, the central axis of the positioning plate, and the central axis of the rotating seat are all on the same straight line, and the perforations on the screening frame and the supporting base plate are evenly distributed in a ring around the central axis of the central support column.

[0010] Preferably, the diameter of the inner wall of the screening frame is greater than the diameter of the perforation, the diameter of the inner wall of the screening frame is equal to the diameter of the feed hole on the top plate, and the diameter of the feed hole on the top plate is equal to the diameter of the through hole on the positioning plate.

[0011] Preferably, at least three limiting stops are provided around the perforation, and the limiting stops are evenly distributed in a ring around the central axis of the perforation, with the central axis of the perforation coinciding with the central axis of the feed hole on the top plate.

[0012] The limiting stop is a round rod, and the outer wall of the limiting stop is tangent to the inner wall of the feed hole.

[0013] Preferably, the diameter of the lifting plate is smaller than the diameter of the perforation in the supporting base plate;

[0014] The filter frame has three sets of outwardly protruding, arc-shaped clamping and clearance openings formed on its frame wall; the clamping and clearance openings are evenly distributed in a ring around the central axis of the filter frame.

[0015] Preferably, the limiting prism is a ring-shaped prism, and planar grooves are formed on the outer walls of the opposite sides of the limiting prism. The base is formed with a hole with the same horizontal cross section as the limiting prism, and the limiting prism is inserted into the hole of the base.

[0016] The length of the protective sleeve is greater than the distance from the upper end face of the positioning plate to the lower end face of the supporting base plate.

[0017] Preferably, the base plate has a plurality of longitudinally distributed mounting holes on its two sides extending out of the side wall of the base.

[0018] The beneficial effects of this invention are as follows:

[0019] This screening and culture device has an automatic screening structure on the top of the built-in rack, which can screen and sort the culture dishes, making it convenient for the robotic arm to take out the specified culture dishes. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0021] Figure 2 This is a three-dimensional structural schematic diagram of the present invention from another angle;

[0022] Figure 3 This is a frontal structural diagram of the present invention;

[0023] Figure 4 This is a side view of the structure of the present invention;

[0024] Figure 5 for Figure 4 Schematic diagram of cross-section at point AA.

[0025] In the diagram: 1. Base; 2. Base plate; 21. Mounting hole; 3. Central support column; 4. Rotary seat; 5. Supporting base plate; 51. Through hole; 6. Limiting stop bar; 7. Top plate; 71. Feed hole; 8. Positioning plate; 81. Through hole; 9. Screening frame; 91. Clamping clearance opening; 10. Spoke; 11. Rotary seat; 12. Rotary motor; 13. Gear ring; 14. Gear; 15. Rotary motor; 16. Lifting plate; 17. Limiting prism; 18. Lead screw; 19. Protective sleeve; 20. Lifting motor. Detailed Implementation

[0026] Example: See Figures 1 to 5As shown in the figure, a screening and culturing device for phosphorus-uptake bacterial strains used in biological phosphorus removal from sewage includes a screening and culturing box. Inside the screening and culturing box, there is a screening and culturing placement rack. The screening and culturing placement rack includes a base 1 that is longitudinal and in the shape of a "冂". A rectangular bottom plate 2 is fixedly connected to the lower end of the base 1. A central support column 3 is fixedly connected to the middle of the bottom plate 2. The central support column 3 passes through the base 1 and is sleeved with a rotating seat 4 and an annular supporting bottom plate 5. The supporting bottom plate 5 is fixed on the upper end surface of the rotating seat 4. The rotating seat 4 is connected to the central support column 3 through a bearing; A number of circular through holes 51 are formed on the supporting bottom plate 5 around the rotating seat 4. A number of annularly distributed limiting stop rods 6 are respectively fixedly connected to the supporting bottom plate 5 around the through holes 51. The upper ends of the limiting stop rods 6 are fixedly connected to an annular top plate 7. A number of feeding holes 71 opposite to the through holes 51 are formed on the outer circle of the top plate 7;

[0027] A circular positioning plate 8 is abutted on the upper end surface of the top plate 7. The upper end of the central support column 3 extends out of the top plate 7 and is inserted and fixed on the positioning plate 8. A through hole 81 opposite to the feeding hole 71 on the top plate 7 is formed on the rear side of the positioning plate 8; A number of circular screening frames 9 opposite to the through hole 81 are abutted on the upper end surface of the outer circle of the positioning plate 8. A radial rod 10 is fixedly connected between adjacent screening frames 9. The inner end of the radial rod 10 is fixedly connected to a T-shaped rotary seat 11. A rotary motor 12 is fixedly connected to the upper end surface of the rotary seat 11. The rotating shaft of the rotary motor 12 is inserted and fixed on the central support column 3. The rotary seat 11 is connected to the upper end of the central support column 3 through a bearing;

[0028] A lifting plate 16 is inserted into the through hole 81 of the positioning plate 8. A limiting prism 17 is fixedly connected to the lower end surface of the lifting plate 16. A vertical threaded hole is formed inside the limiting prism 17; The lower end of the limiting prism 17 passes through the feeding hole 71 of the top plate 7 and the through hole 51 of the supporting bottom plate 5 and is inserted on the base 1; A protective sleeve 19 opposite to the limiting prism 17 is fixedly connected to the lower end surface at the rear side of the bottom plate 2. A lead screw 18 is inserted into the protective sleeve 19. The upper end of the lead screw 18 is screwed into the threaded hole of the limiting prism 17, and the lower end is fixedly connected to the rotating shaft of the lifting motor 20 through a coupling. The lifting motor 20 is fixedly connected to the bottom of the protective sleeve 19.

[0029] A gear ring 13 is inserted and fixedly connected to the lower end of the rotating seat 4. A gear 14 is meshed with the front side of the gear ring 13; A rotating motor 15 is fixedly connected to the base 1. The rotating shaft of the rotating motor 15 extends out of the base 1 and is inserted and fixed inside the gear 14; The rotating motor 15 can drive the rotating seat 4 to rotate based on gear transmission. The rotating seat 4 drives the supporting bottom plate 5 to rotate. Circular culture dishes arranged in layers can be stored on the supporting bottom plate 5 between the limiting stop rods 6. The rotation of the supporting bottom plate 5 can move the circular culture dishes to directly below the through hole 81 of the positioning plate 8.

[0030] The lead screw 18 and the protective sleeve 19 are both located on the rear side of the gear ring 13. The base plate 2 is formed with clearance holes opposite to the lead screw 18 and the limiting prism 17. When the protective sleeve 19 moves down, it can pass through the clearance holes and enter the protective sleeve 19.

[0031] The central axis of the central support column 3, the central axis of the rotating seat 4, the central axis of the supporting base plate 5, the central axis of the positioning plate 8, and the central axis of the rotating seat 11 are all on the same straight line. The perforations 51 on the screening frame 9 and the supporting base plate 5 are evenly distributed in a ring around the central axis of the central support column 3.

[0032] The diameter of the inner wall of the screening frame 9 is greater than the diameter of the perforation 51. The diameter of the inner wall of the screening frame 9 is equal to the diameter of the feed hole 71 on the top plate 7. The diameter of the feed hole 71 on the top plate 7 is equal to the diameter of the through hole 81 on the positioning plate 8. The circular culture dish needs to pass through the feed hole 71 and the through hole 81 to be removed from the screening culture placement rack under the lifting action of the lifting plate 16.

[0033] At least three limiting rods 6 are provided around the perforation 51. The limiting rods 6 are evenly distributed in a ring around the central axis of the perforation 51. The central axis of the perforation 51 coincides with the central axis of the feed hole 71 on the top plate 7. The limiting rods 6 can be used to position the circular culture dish and prevent it from falling off the screening culture placement rack.

[0034] The limiting rod 6 is a round rod, and the outer wall of the limiting rod 6 is tangent to the inner wall of the feed hole 71. In addition to the limiting function mentioned above, the limiting rod 6 also has a guiding function, which can prevent interference between the round culture dish and the top plate 7 during the upward movement of the culture dish.

[0035] The diameter of the lifting plate 16 is smaller than the diameter of the through hole 51 on the supporting base plate 5;

[0036] The screening frame 9 has three sets of outwardly protruding, arc-shaped clamping and clearance openings 91 formed on its frame wall. The clamping and clearance openings 91 are evenly distributed in a ring around the central axis of the screening frame 9. The clamping and clearance openings 91 are equipped with grippers that can be inserted into the grippers, so that the circular culture dishes can be grasped and placed by the grippers.

[0037] The limiting prism 17 is a ring-shaped prism. Planar grooves are formed on the outer walls of the opposite sides of the limiting prism 17. The base 1 has a hole with the same horizontal cross section as the limiting prism 17. The limiting prism 17 is inserted into the hole of the base 1.

[0038] The length of the protective sleeve 19 is greater than the distance from the upper end face of the positioning plate 8 to the lower end face of the supporting base plate 5.

[0039] The base plate 2 extends from the side wall of the base 1 on both sides and has several longitudinally distributed mounting holes 21. Bolts are installed in the mounting holes 21, and the base plate 2 can be fixedly connected to the screening incubator by bolts.

[0040] Working principle: This structure is a screening and cultivation device for phosphorus-absorbing bacteria for biological phosphorus removal in wastewater. The top of the screening and cultivation device is equipped with a positioning plate 8, a screening frame 9, a spoke 10 and a rotating seat 11.

[0041] Under normal circumstances, if the circular culture dish placed on the top of the support base plate 5 in the placement rack rotates under the action of the rotating seat 4, the circular culture dish on its top is moved to the direct below the through hole 81 of the positioning plate 8, and then the circular culture dish on its top is moved out of the positioning plate 8 and into the screening frame 9 by the upward movement of the limiting prism 17, and the robot can grab the circular culture dish in the screening frame 9.

[0042] When the specified circular culture dish is not at the top, the column containing the specified circular culture dish is moved directly below the through hole 81. After the circular culture dish is moved into the screening box 9, the rotary motor 12 is started. Based on the screening box 9, the circular culture dish can be moved above the positioning plate 8. Then the limiting prism 17 pushes the next circular culture dish into the next screening box 9. Through the step-by-step pushing of the limiting prism 17 and the step-by-step movement of the screening box 9, the specified circular culture dish is moved into the screening box 9 directly above the through hole 81, where it can be grasped by a mechanical gripper.

[0043] Furthermore, the other circular culture dishes set on the positioning plate 8 can be moved one by one to the lifting plate 16, and then the lifting plate 16 moves down layer by layer, and then the circular culture dishes in the screening box 9 move to the top of the through hole 81; gradually realizing the reset of the circular culture dishes.

[0044] The embodiments described are illustrative of the invention and are not intended to limit the invention. Any person skilled in the art can modify the embodiments without departing from the spirit and scope of the invention; therefore, the scope of protection of the invention should be as set forth in the claims.

Claims

1. A screening and cultivation device for phosphorus-absorbing bacteria strains for biological phosphorus removal in wastewater, comprising a screening culture box, a screening culture placement rack inside the screening culture box, the screening culture placement rack comprising a longitudinally arranged "U"-shaped base (1), a rectangular base plate (2) fixedly connected to the lower end of the base (1), a central support column (3) fixedly connected to the middle of the base plate (2), the central support column (3) passing through the base (1) and fitted with a rotating seat (4) and an annular supporting base plate (5), the supporting base plate (5) being fixed to the upper surface of the rotating seat (4), the rotating seat (4) being connected to the central support column (3) via bearings; characterized in that: The rotating seat (4) has several circular perforations (51) formed on the supporting base plate (5) around it. Several ring-shaped limiting rods (6) are fixedly connected to the supporting base plate (5) around the perforations (51). A ring-shaped top plate (7) is fixedly connected to the upper end of the limiting rod (6). Several feed holes (71) are formed on the outer ring of the top plate (7) and are directly opposite to the perforations (51). A circular positioning plate (8) is abutted on the upper surface of the top plate (7). The upper end of the central support column (3) extends out of the top plate (7) and is inserted and fixed on the positioning plate (8). A through hole (81) is formed on the rear side of the positioning plate (8) opposite to the feed hole (71) on the top plate (7). Several circular screening frames (9) are abutted on the upper surface of the outer ring of the positioning plate (8) and opposite to the through hole (81). A spoke (10) is fixedly connected between adjacent screening frames (9). The inner end of the spoke (10) is fixedly connected to the T-shaped rotary seat (11). A rotary motor (12) is fixedly connected to the upper surface of the rotary seat (11). The shaft of the rotary motor (12) is inserted and fixed on the central support column (3). The rotary seat (11) is connected to the upper end of the central support column (3) through a bearing. The positioning plate (8) has a through hole (81) into which a lifting plate (16) is inserted. A limiting prism (17) is fixedly attached to the lower end face of the lifting plate (16). A vertical threaded hole is formed in the limiting prism (17). The lower end of the limiting prism (17) passes through the feed hole (71) of the top plate (7) and the through hole (51) of the supporting base plate (5) and is inserted into the base (1). A protective sleeve (19) opposite to the limiting prism (17) is fixedly attached to the lower end face of the rear side of the base plate (2). A lead screw (18) is inserted in the protective sleeve (19). The upper end of the lead screw (18) is screwed into the threaded hole of the limiting prism (17), and the lower end is fixedly connected to the shaft of the lifting motor (20) through a coupling. The lifting motor (20) is fixedly attached to the bottom of the protective sleeve (19). The screening culture placement rack contains a circular culture dish. The circular culture dish rotates under the action of the rotating seat (4). The circular culture dish at the top of the screening culture placement rack moves to the bottom of the through hole (81) of the positioning plate (8). The circular culture dish at the top of the screening culture placement rack is lifted to the screening frame (9) above the positioning plate (8) by the upward movement of the limiting prism (17). The rotary motor (12) is started, and the screening frame (9) rotates on the positioning plate (8) to transfer the circular culture dish to the positioning plate (8). By inserting the limiting prisms (17) one by one and moving the screening frames (9) one by one, the circular culture dishes are distributed on the positioning plate (8). When the specified circular culture dish enters the screening frame (9) above the through hole (81), the robot arm grabs the specified circular culture dish from the screening frame (9). After the specified circular culture dish is grabbed, the circular culture dishes distributed on the positioning plate (8) are transferred to the screening culture placement rack one by one and reset by moving the screening frames (9) one by one and moving the lifting plate (16) layer by layer.

2. The screening and cultivation device for phosphorus-absorbing bacteria for biological phosphorus removal in wastewater according to claim 1, characterized in that: The lower end of the rotating seat (4) is fitted with a gear ring (13), and the front side of the gear ring (13) is engaged with a gear (14); a rotary motor (15) is fixedly connected to the base (1), and the shaft of the rotary motor (15) extends out of the base (1) and is inserted and fixed inside the gear (14). The lead screw (18) and the protective sleeve (19) are both located on the rear side of the gear ring (13), and the bottom plate (2) is formed with clearance holes opposite to the lead screw (18) and the limiting prism (17).

3. The screening and cultivation device for phosphorus-absorbing bacteria strains for biological phosphorus removal in wastewater according to claim 1, characterized in that: The central axis of the central support column (3), the central axis of the rotating seat (4), the central axis of the supporting base plate (5), the central axis of the positioning plate (8), and the central axis of the rotating seat (11) are all on the same straight line. The perforations (51) on the screening frame (9) and the supporting base plate (5) are evenly distributed in a ring around the central axis of the central support column (3).

4. The screening and cultivation device for phosphorus-absorbing bacteria strains for biological phosphorus removal in wastewater according to claim 3, characterized in that: The diameter of the inner wall of the screening frame (9) is greater than the diameter of the through hole (51). The diameter of the inner wall of the screening frame (9) is equal to the diameter of the feed hole (71) on the top plate (7). The diameter of the feed hole (71) on the top plate (7) is equal to the diameter of the through hole (81) on the positioning plate (8).

5. The screening and cultivation device for phosphorus-absorbing bacteria strains for biological phosphorus removal in wastewater according to claim 3, characterized in that: At least three limiting rods (6) are provided around the perforation (51). The limiting rods (6) are evenly distributed in a ring around the central axis of the perforation (51). The central axis of the perforation (51) coincides with the central axis of the feed hole (71) on the top plate (7). The limiting stop (6) is a round rod, and the outer wall of the limiting stop (6) is tangent to the inner wall of the feed hole (71).

6. The screening and cultivation device for phosphorus-absorbing bacteria for biological phosphorus removal in wastewater according to claim 4, characterized in that: The diameter of the lifting plate (16) is smaller than the diameter of the through hole (51) on the supporting base plate (5); The screening frame (9) has three sets of outwardly protruding, arc-shaped clamping and clearance openings (91) formed on its frame wall; the clamping and clearance openings (91) are evenly distributed in a ring around the central axis of the screening frame (9).

7. The screening and cultivation device for phosphorus-absorbing bacteria for biological phosphorus removal in wastewater according to claim 1, characterized in that: The limiting prism (17) is a ring-shaped prism. Planar grooves are formed on the outer walls of the two opposite sides of the limiting prism (17). The base (1) has a socket with the same horizontal cross section as the limiting prism (17). The limiting prism (17) is inserted into the socket of the base (1). The length of the protective sleeve (19) is greater than the distance from the upper end face of the positioning plate (8) to the lower end face of the supporting base plate (5).

8. The screening and cultivation device for phosphorus-absorbing bacteria for biological phosphorus removal in wastewater according to claim 1, characterized in that: The base plate (2) has several longitudinally distributed mounting holes (21) extending from the side walls of the base (1) on both sides.

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