Experimental tabulation system and method of sanger sequencer

Abstract

The invention discloses an experiment table compiling system and method for a sanger sequencer, and the system comprises an experiment method: determining an experiment type, a dye set, a sequencing analysis model and experiment model information in an established experiment method, binding and summarizing the experiment model, the dye set and the sequencing analysis model, and forming an experiment method; sample file names and sample file storage position information are determined in the established sample files, and the sample file names and the sample file storage positions are bound and summarized to form one sample file; according to the kit, an analysis method is defined in the established kit, Panel and interior label information is analyzed, and the analysis method, the Panel and the interior label are bound and summarized to form the kit; and a runtime library: binding and summarizing the established experimental methods, sample files and kits to form the runtime library. According to the invention, the user only needs to select the runtime library for one time to complete the tabulation processing of the experiment, so that errors caused by selection are reduced.

Description

TECHNICAL FIELD

[0001] The application relates to an experimental tabulation system and method of a Sanger sequencer and belongs to the technical field of data arrangement. BACKGROUND

[0002] In Sanger sequencing, software and users are generally used for interaction, and when specific to an experiment, various experimental parameters, dyes, sample naming methods, sample file storage positions, analysis methods used by samples, used panels and internal standards need to be defined and selected by the user. However, each needs to be selected by the user when establishing a form, thereby reducing the operation efficiency of the user, and too many parameters can cause the user to make errors. In actual application, some software can combine some parameters, such as combining a running model and dyes into one parameter. However, the selection times of the user cannot be completely reduced. SUMMARY

[0003] At least in view of one problem of the prior art, the application provides an experimental tabulation system and method of a Sanger sequencer, which can greatly reduce the workload of the user and reduce errors caused by selection.

[0004] In order to achieve the above-mentioned purpose, the application adopts the following technical scheme: an experimental tabulation system of a Sanger sequencer, comprising an experimental method, a sample file, a kit and a running library:

[0005] The experimental method: in the established experimental method, the experimental type, the dye set, the sequencing analysis model and the experimental model information are determined, the experimental model, the dye set and the sequencing analysis model are bound and summarized, an experimental method is formed, and the experimental method is used for sample experiment;

[0006] The sample file: in the established sample file, the sample file naming and the sample file storage position information are determined, the sample file naming and the sample file storage position are bound and summarized, a sample file is formed, and the sample file is used for saving sample experiment information;

[0007] The kit: in the established kit, the analysis method, the analysis panel and the internal standard information are determined, the analysis method, the panel and the internal standard are bound and summarized, a kit is formed, and the kit is used for sample detection;

[0008] The running library: the established experimental method, the sample file and the kit are bound and summarized, a running library is formed, and the running library is used for experimental tabulation processing.

[0009] Preferably, the sample file naming is set through a naming rule, a rule name control is additionally provided, a suitable rule name is selected according to needs to set the sample file naming, and a sample folder with a determined name is formed.

[0010] Preferably, the rule name control includes date, time, experiment name, run group, dial, disk hole, hole number, sample name, lane number, separator, and fixed text, etc.

[0011] Preferably, the sample file storage location adds a storage path option, and the storage path option includes a default file storage path and a custom storage path.

[0012] Preferably, the storage path option includes a default file storage path and a custom storage path.

[0013] Preferably, in the experiment method, the experiment method name is set by sequentially combining the information of the experiment model, the dye set, and the sequencing analysis model.

[0014] Preferably, in the kit, the kit name is set by analyzing the locus information of the Panel.

[0015] The method of the experiment tabulation system of the sanger sequencer comprises the following steps:

[0016] S1, first establish an experiment method: clear the experiment type, dye set, sequencing analysis model, and experiment model information, bind and combine the experiment type, dye set, sequencing analysis model, and experiment model to form an experiment method, and the experiment method name is set by sequentially combining the names of the experiment model, dye set, and sequencing analysis model;

[0017] S2, establish a sample file:

[0018] S2.1 Sample file naming: first set by naming rules, add a rule name control, which includes date, time, experiment name, run group, dial, disk hole, hole number, sample name, lane number, separator, and fixed text, etc., which can support selecting rule name to set sample file naming, and form a sample folder with a certain name;

[0019] S2.2 Sample file storage location: add a storage path option, which includes a default file storage path and a custom storage path, which can support selecting a storage path to set the storage location of the sample folder with a certain name;

[0020] S3, build a kit: clear the analysis method, analysis Panel, and internal standard information, bind and combine the analysis method, Panel, and internal standard to form a kit, and the kit name is set by the locus information of the Panel.

[0021] S4, establish a run library: bind and combine the established experiment method, sample file, and kit to form a run library.

[0022] S5, set the experimental method, sample file and related parameters in the kit in advance, only select the run library to process the experiment table.

[0023] The beneficial effects of the present application are: 1. The present application sets a run library, binds and collects the experimental method, sample file and kit, as long as the user sets the related parameters in advance; 2. The related parameters in the present application include experimental model, dye set, sample file naming, sample file storage location, analysis method, analysis panel and internal standard, which are merged into a run library by a step-by-step merging manner; 3. In the present application, the user only needs to select the run library once to complete the experiment table processing, which greatly reduces the workload of the user and reduces the errors caused by selection. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a whole system control structure schematic diagram of the embodiment of the present application;

[0025] Figure 2 It is an experimental method related parameter editing schematic diagram of the embodiment of the present application;

[0026] Figure 3 It is a sample file naming rule setting schematic diagram of the embodiment of the present application;

[0027] Figure 4 It is a sample file folder storage schematic diagram of the embodiment of the present application;

[0028] Figure 5 It is a kit setting schematic diagram of the embodiment of the present application;

[0029] Figure 6 It is a run library setting schematic diagram of the embodiment of the present application;

[0030] Figure 7 It is an experimental table processing result schematic diagram of the embodiment of the present application. DETAILED DESCRIPTION

[0031] The following is a clear and complete description of the technical solutions in the embodiments of the present application, and the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0032] As shown in the figure, the embodiment of the present application provides an experimental table processing system of sanger sequencer, including experimental method, sample file, kit and run library: Figure 1

[0033] ​Experimental method: in the established experimental method, the experimental type, dye set, sequencing analysis model and experimental model information are determined, the experimental model, dye set and sequencing analysis model are bound and summarized to form an experimental method; the experimental method name is set by combining the information of the experimental model, dye set and sequencing analysis model in turn; the experimental method is used for sample experiment;

[0034] Sample file: in the established sample file, the sample file naming and sample file storage location information are determined, the sample file naming and sample file storage location are bound and summarized to form a sample file; the sample file naming is set through a naming rule, a rule name control is added, the sample file naming is set according to the need by selecting a suitable rule name, and a sample file folder with a determined name is formed; the rule name control includes date, time, experiment name, running group, dial, disk hole, hole position number, sample name, lane number, separator and fixed text; the sample file storage location adds a storage path option, and the storage path of the sample file folder is set according to the need by selecting a suitable storage path; the storage path option includes a default file storage path and a custom storage path; the sample file is used for saving sample experimental information;

[0035] The kit: in the established kit, the analysis method, analysis Panel and internal standard information are determined, the analysis method, Panel and internal standard are bound and summarized to form a kit; the kit name is set through the locus information of the analysis Panel; the kit is used for sample detection;

[0036] Run library: the established experimental method, sample file and kit are bound and summarized to form a run library, and tabulation processing is performed after sample experiment is completed.

[0037] Nomenclature of the present application:

[0038] The experimental model is a parameter sent by the software to the instrument, such as voltage, temperature, laser power, etc.

[0039] The dye set is the type of dye carried by the sample in this experiment;

[0040] The sample file naming is the naming rule of the sample name in this experiment;

[0041] The sample file storage location is the storage location of the file in this experiment;

[0042] The analysis method is the analysis method of the experimental kit used in this experiment;

[0043] The analysis PANEL is the locus information of the kit used in this experiment;

[0044] The internal standard, i.e. the molecular weight standard, is the internal standard parameter used in this experiment.

[0045] As Figures 2 to 7 shown, the method of the experimental tabulation system of the sanger sequencer includes the following steps,

[0046] S1, first establish an experimental method: clear the experimental type, dye set, sequencing analysis model and experimental model information, bind the experimental type, dye set, sequencing analysis model and experimental model, form an experimental method, and the experimental method name is set in the name of the experimental model, dye set and sequencing analysis model, such as Figure 1 As shown;

[0047] S2, establish a sample file:

[0048] S2.1 sample file naming: first set through the naming rule, add rule name control, rule name control includes date, time, experiment name, running group, dial, disk hole, hole position number, sample name, lane number, separator and fixed text, etc., which can support selecting rule name to set sample file naming, form a sample folder with a certain name, such as Figure 2 As shown;

[0049] S2.2 sample file storage location: add storage path option, which includes default file storage path and custom storage path, which can support selecting storage path to set the storage location of the sample folder with a certain name, such as Figure 3 As shown;

[0050] S3, build a kit: clear the analysis method, analysis Panel and internal standard information, bind and summarize the analysis method, Panel and internal standard, form a kit, and the kit name is set with the locus information of the Panel, such as Figure 4 As shown;

[0051] S4, establish a running library: bind and summarize the established experimental method, sample file and kit, form a running library, such as Figure 5 As shown;

[0052] S5, set the related parameters in the experimental method, sample file and kit in advance, only select the running library to process the experimental tabulation, such as Figure 6 As shown.

[0053] The application sets a running library, the user sets the related parameters in advance: running model, dye set, sample naming method, sample file storage location, analysis method, analysis PANEL, internal standard, then merges the seven parameters into a running library through the way of step-by-step merging, the user only needs to select the running library once to complete the tabulation processing of the experiment, greatly reducing the workload of the user and reducing the errors caused by selection.

[0054] It is apparent for a person skilled in the art that the present application is not limited to the details of the above-described exemplary embodiments, but that it can be implemented in other concrete forms without departing from the spirit and essential characteristics of the present application. Therefore, the embodiments should be considered in all respects as illustrative and not restrictive, the scope of the present application being defined by the appended claims rather than the above description, and all changes falling within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

[0055] Furthermore, it should be understood that although the present specification describes exemplary embodiments, the present specification does not limit the application to only the described exemplary embodiments. The present specification describes the exemplary embodiments with sufficient details to a person skilled in the art, and the person skilled in the art should consider the present specification as a whole, and the technical solutions in the exemplary embodiments can be combined appropriately to form other embodiments that can be understood by the person skilled in the art.

Claims

1. An experimental cataloging system for a Sanger sequencer, characterized in that, Includes experimental methods, sample files, reagent kits, and runtime libraries: The experimental method described is as follows: the experimental type, dye set, sequencing analysis model, and experimental model information are clearly defined in the established experimental method. The experimental model, dye set, and sequencing analysis model are bound and summarized to form an experimental method for the experiment on the sample. The sample file: The sample file name and sample file save location information are specified in the established sample file. The sample file name and sample file save location are bound and summarized to form a sample file for saving sample experimental information. The kit: The analytical method, analytical panel and internal standard information are clearly defined in the established kit. The analytical method, panel and internal standard are bound and summarized to form a kit for sample detection. The runtime library: It binds and summarizes the established experimental methods, sample files, and reagent kits to form a runtime library, which is used for experimental table processing.

2. The experimental cataloging system for a Sanger sequencer according to claim 1, characterized in that, The sample files are named according to naming rules. A rule name control is added, and the appropriate rule name is selected as needed to set the sample file name, forming a sample folder with a specific name.

3. The experimental cataloging system for a Sanger sequencer according to claim 2, characterized in that, The rule name control includes date, time, experiment name, run group, dial, disk hole, well number, sample name, lane number, separator, and fixed text.

4. The experimental cataloging system for a Sanger sequencer according to claim 2, characterized in that, The sample file storage location has been updated to include a storage path option, allowing users to select a suitable storage path to set the storage location of the sample folder as needed.

5. The experimental cataloging system for a Sanger sequencer according to claim 4, characterized in that, The storage path options include a default file storage path and a custom storage path.

6. The experimental cataloging system for a Sanger sequencer according to claim 1, characterized in that, In the experimental method described, the experimental method name is determined by sequentially combining information from the experimental model, dye set, and sequencing analysis model.

7. The experimental cataloging system for a Sanger sequencer according to claim 1, characterized in that, The kit name is determined by analyzing the gene locus information of the panel.

8. The method for experimental cataloging of the Sanger sequencer according to any one of claims 1 to 7, characterized in that, Includes the following steps: S1. First, establish the experimental method: clarify the experimental type, dye set, sequencing analysis model, and experimental model information, bind and combine the experimental type, dye set, sequencing analysis model, and experimental model to form an experimental method, and set the name of the experimental method by combining the names of the experimental model, dye set, and sequencing analysis model in sequence. S2. Create sample files: S2.1 Sample file naming: First, set the naming rules and add a rule name control. The rule name control includes date, time, experiment name, run group, dial, disk hole, well position number, sample name, lane number, separator, and fixed text, etc. It can support selecting the rule name to set the sample file name and form a sample folder with a fixed name. S2.2 Sample file save location: Added storage path options, including default file storage path and custom storage path, which can support selecting the storage path to set the save location of sample folders with specific names; S3. Establish a reagent kit: Define the analytical methods, analytical panels and internal standard information, bind and summarize the analytical methods, panels and internal standards to form a reagent kit, and set the name of the reagent kit based on the gene locus information of the panel; S4. Create a runtime library: Bind and summarize the established experimental methods, sample files, and reagent kits to form a runtime library; S5. Pre-set the experimental methods, sample files, and relevant parameters in the reagent kit, and only select the runtime library for experimental table processing.

Images