Composition for promoting metabolism of hair papilla cells as well as preparation method and application of composition

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition for promoting metabolism of hair papilla cells as well as a preparation method and application of the composition. The composition disclosed by the invention is prepared from the following components: a cacumen biotae extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4. The components in the composition have a good synergistic effect, the mitochondrial membrane potential of the hair papilla cells can be obviously enhanced, the cell activity of the hair papilla cells is promoted, the cell metabolism function of the hair papilla cells is promoted, and therefore the health of hair follicles is effectively maintained.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a composition for promoting metabolism of hair papilla cells, a preparation method and application thereof. BACKGROUND

[0002] Hair follicle is an appendage of the epidermis of the scalp, which maintains the normal function of hair follicle stem cells by maintaining the periodic cycle of hair follicle stem cells in the anagen, catagen and telogen phases. Hair papilla cells are located at the bottom of the hair follicle and are the core cell group for regulation in the hair growth cycle. Hair papilla cells transmit the start signal of the hair follicle cycle to the papillary and secondary germinal zones by secreting a series of key signal molecules, and directly regulate the hair follicle cycle (anagen, catagen and telogen). The vitality of hair papilla cells directly affects the hair growth rate, density and anti-shedding ability. The functional decline of hair papilla cells is one of the key inducers of hair loss (such as androgenetic alopecia and stress-induced alopecia).

[0003] Mitochondria, as the core organelle of cell energy supply, provide the necessary energy for the life activities of hair papilla cells by efficiently producing adenosine triphosphate. Hair papilla cells need a large amount of energy to continuously synthesize and secrete a variety of signal molecules necessary for activating hair follicle stem cells and starting and maintaining the hair growth cycle, such as Wnt. The energy metabolism function of mitochondria supports various life activities of hair papilla. Dehydrogenase in mitochondria catalyzes the dehydrogenation of substrates in the tricarboxylic acid cycle and the electron transport chain, driving the efficient synthesis of ATP, providing the necessary energy for hair papilla cells to regulate the hair follicle growth cycle, synthesize signal molecules and maintain their high metabolic requirements. The dehydrogenase activity of mitochondria is essential for mitochondria to maintain normal energy metabolism, which can be detected by CCK-8 and other methods. In addition, mitochondria also maintain the homeostasis and health of hair papilla cells by regulating metabolic intermediates and maintaining cellular reactive oxygen species balance. Mitochondrial membrane potential, as one of the basic characteristics of mitochondria, refers to the transmembrane potential difference formed by the proton concentration gradient and electrochemical gradient between the inner and outer membranes of mitochondria maintained by the proton pump. This potential difference is the energy basis for mitochondria to synthesize "energy currency" adenosine triphosphate. Healthy mitochondrial function is the basis for hair papilla cells to effectively support hair follicle stem cell differentiation, drive the hair follicle into the hair growth cycle and maintain its vigorous growth ability. Once the mitochondrial function of hair papilla cells is impaired, the energy supply of the cells is insufficient or the oxidative stress is imbalanced, which will directly weaken the signal hub role of hair papilla cells in the hair follicle, leading to disorder of the hair follicle cycle, inhibition of hair growth and even hair loss.

[0004] Current hair care products focus on maintaining hair follicle health through anti-inflammatory, antioxidant, and 5α-reductase inhibition, but do not directly target the core of hair follicle cell health, failing to fundamentally address the problem of hair follicle health imbalance. Therefore, how to effectively regulate and maintain the mitochondrial function of hair papilla cells to maintain their normal energy metabolism function and other physiological functions is a technical problem that needs to be solved by those skilled in the art. SUMMARY

[0005] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a composition for promoting hair papilla cell metabolism, as well as a preparation method and application thereof, which can effectively enhance the mitochondrial membrane potential of hair papilla cells and maintain hair follicle health.

[0006] In a first aspect, the present application provides a composition comprising the following components: cypress leaf extract, trehalose, betaine, acetyl tetrapeptide-3, and myristoyl pentapeptide-4.

[0007] Specifically, cypress leaf extract is an important anti-hair loss raw material widely used in hair care products. Traditional research on the efficacy of cypress leaf extract has mainly focused on its anti-inflammatory, antioxidant, and antibacterial properties. However, the present application is based on the important role of cypress leaf extract in maintaining hair follicle health and cell function.

[0008] Betaine is a highly effective osmoprotectant and methyl donor that helps cells maintain osmotic pressure balance, stabilize protein structure, and reduce environmental stress damage to cells, thereby creating a more stable internal environment for cells (including mitochondria) and supporting their efficient operation.

[0009] Specifically, trehalose is a natural biological protective agent with excellent water retention and cell membrane stabilizing effects. It can form a protective layer around cells (especially mitochondrial membranes), enhance cell tolerance and survival rate under stress, and help maintain mitochondrial membrane integrity and functional stability, providing a fundamental guarantee for energy metabolism.

[0010] Specifically, acetyl tetrapeptide-3 is a signal peptide that is generally believed to help stimulate the synthesis of structural proteins (such as collagen IV and laminin) at the dermal-epidermal junction (DEJ), enhance the stability of the hair follicle anchoring structure, and indirectly provide a healthier microenvironment for hair papilla cells.

[0011] Specifically, myristoyl pentapeptide-4 is a lipopeptide commonly used to promote the synthesis of extracellular matrix components (such as collagen, fibronectin, and glycosaminoglycans). It helps improve the microcirculation and nutrient supply around the hair follicle, supports the matrix environment for the survival and function of hair papilla cells, and indirectly assists in maintaining their energy metabolism and vitality.

[0012] As a traditional anti-hair loss raw material, the application has carried out in-depth research and expansion on the effect level of cypress leaves on hair papilla cells, and found that it can enhance the mitochondrial membrane potential of hair papilla cells, improve the metabolic capacity of hair papilla cells, promote the normal function and cell viability of hair papilla cells, and effectively maintain the health of hair follicles. The application further enhances the activation of cypress leaf extract on the mitochondrial function of hair papilla cells through the synergistic effect of betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4, so as to realize the obvious improvement of the metabolic capacity of hair papilla cells. That is, the composition described in the application takes cypress leaf extract as the core, enhances the energy metabolism capacity of hair papilla cells, and improves this effect through the synergistic effect of betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4, and realizes the obvious improvement of the energy metabolism capacity of hair papilla cells through the mutual cooperation and synergistic effect of various components.

[0013] In some embodiments of the application, the mass ratio of the cypress leaf extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 is 0.1:(0.01-0.1):(0.01-0.1):(0.000001-0.0001):(0.000001-0.0001).

[0014] In some embodiments of the application, the mass ratio of the cypress leaf extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 is 0.1:(0.01-0.1):(0.01-0.1):(0.00001-0.0001):(0.00001-0.0001).

[0015] In a second aspect of the application, a preparation method of the composition of the first aspect of the application is provided, comprising the following steps:

[0016] Mixing the components to obtain the composition.

[0017] In a third aspect of the application, the application of the composition of the first aspect of the application in preparing a product with anti-hair loss effect is provided.

[0018] In some embodiments of the application, the product includes a hair care product or an external skin preparation.

[0019] In some embodiments of the application, the anti-hair loss effect includes promoting the cell viability of hair papilla cells and / or promoting the cell metabolic function of hair papilla cells.

[0020] In a fourth aspect of the application, a hair care product is provided, which includes the composition of the first aspect of the application.

[0021] In some embodiments of the present application, the mass content of the composition in the wash care product is 0.01%-1%. The mass content of the composition can be any point value or any range value between 0.01%-1%, such as 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.

[0022] In some embodiments of the present application, the mass content of the composition in the wash care product is 0.1%-0.5%.

[0023] In some embodiments of the present application, the mass content of the composition in the wash care product is 0.0001%-0.1%. The mass content of the composition can be any point value or any range value between 0.0001%-1%, such as 0.0001%, 0.01%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.

[0024] In some embodiments of the present application, the mass content of the composition in the wash care product is 0.01%-0.1%. The mass content of the composition can be any point value or any range value between 0.01%-1%, such as 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.

[0025] In some embodiments of the present application, the mass content of the composition in the wash care product is 0.01%-0.1%. The mass content of the composition can be any point value or any range value between 0.01%-1%, such as 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.

[0026] In some embodiments of the present application, the mass content of the composition in the wash care product is 0.000001%-0.0001%. The mass content of the composition can be any point value or any range value between 0.000001%-0.0001%, such as 0.000001%, 0.000005%, 0.00001%, 0.00005%, 0.0001%, etc.

[0027] In some embodiments of the present application, the mass content of the myristoyl pentapeptide-4 in the hair care product is 0.000001%-0.0001%. The mass content of the myristoyl pentapeptide-4 can be any point value or any two-point range value between 0.000001%-0.0001%, such as 0.000001%, 0.000005%, 0.00001%, 0.00005%, 0.0001%, etc.

[0028] In some embodiments of the present application, the hair care product includes shampoo, hair care spray, hair oil, hair conditioner, or hair mask.

[0029] Compared with the prior art, the present application has the following advantages:

[0030] The composition of the present application contains biota leaf extract, trehalose, betaine, acetyl tetrapeptide-3, and myristoyl pentapeptide-4. Through this unique combination of formulations, good synergies between the components are produced, which can significantly enhance the mitochondrial membrane potential of hair papilla cells, promote the cell viability of hair papilla cells, and promote the cell metabolic function of hair papilla cells, thereby effectively maintaining the health of hair follicles. DETAILED DESCRIPTION

[0031] The content of the present application is further described in detail through specific examples. Unless otherwise specified, the raw materials, reagents, or devices used in the examples can be obtained from conventional commercial channels or can be obtained by existing technical methods. Unless otherwise specified, the test or test method is a conventional method in the art.

[0032] Example 1

[0033] A composition is formed by mixing biota leaf extract, betaine, trehalose, acetyl tetrapeptide-3, and myristoyl pentapeptide-4 in a mass ratio of 0.1:0.05:0.05:0.00005:0.00001.

[0034] Example 2

[0035] A composition is formed by mixing biota leaf extract, betaine, trehalose, acetyl tetrapeptide-3, and myristoyl pentapeptide-4 in a mass ratio of 0.1:0.01:0.05:0.00005:0.00001.

[0036] Example 3

[0037] A composition is formed by mixing biota leaf extract, betaine, trehalose, acetyl tetrapeptide-3, and myristoyl pentapeptide-4 in a mass ratio of 0.1:0.1:0.05:0.00005:0.00001.

[0038] Example 4

[0039] A composition, wherein Plukenetia volubilis extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 are mixed in a mass ratio of 0.1:0.05:0.01:0.00005:0.00001.

[0040] Example 5

[0041] A composition, wherein Plukenetia volubilis extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 are mixed in a mass ratio of 0.1:0.05:0.1:0.00005:0.00001.

[0042] Example 6

[0043] A composition, wherein Plukenetia volubilis extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 are mixed in a mass ratio of 0.1:0.05:0.05:0.00001:0.00001.

[0044] Example 7

[0045] A composition, wherein Plukenetia volubilis extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 are mixed in a mass ratio of 0.1:0.05:0.05:0.0001:0.00001.

[0046] Example 8

[0047] A composition, wherein Plukenetia volubilis extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 are mixed in a mass ratio of 0.1:0.05:0.05:0.00005:0.000001.

[0048] Example 9

[0049] A composition, wherein Plukenetia volubilis extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 are mixed in a mass ratio of 0.1:0.05:0.05:0.00005:0.0001.

[0050] Example 10

[0051] A composition, wherein Plukenetia volubilis extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 are mixed in a mass ratio of 0.1:0.01:0.01:0.000001:0.000001.

[0052] Example 11

[0053] A composition is mixed by cypress leaf extract, betaine, trehalose, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 according to the mass ratio of 0.1:0.1:0.1:0.0001:0.0001.

[0054] Comparative Example 1

[0055] The difference from Example 1 is only that the cypress leaf extract is replaced by an equal amount of Saururus chinensis extract.

[0056] Comparative Example 2

[0057] The difference from Example 1 is only that the betaine is replaced by an equal amount of urea.

[0058] Comparative Example 3

[0059] The difference from Example 1 is only that the trehalose is replaced by an equal amount of sorbitol.

[0060] Comparative Example 4

[0061] The difference from Example 1 is only that the acetyl tetrapeptide-3 is replaced by an equal amount of histidine.

[0062] Comparative Example 5

[0063] The difference from Example 1 is only that the myristoyl pentapeptide-4 is replaced by an equal amount of histidine.

[0064] Composition efficacy test

[0065] The compositions of Examples 1-11 were mixed with DMEM medium respectively to prepare test samples 1-11; the compositions of Comparative Examples 1-5 were mixed with DMEM medium respectively to prepare comparative test samples 1-5; the mass content of the efficacy components in each test sample / comparative test sample in DMEM medium is shown in Table 1.

[0066] Table 1

[0067]

[0068]

[0069] 1. Promote hair papilla cell viability efficacy test

[0070] The experimental steps are as follows: KNU201 immortalized hair papilla cells were inoculated in a 96-well plate, the cell density was adjusted to 7×10 4 cells / mL, a standard 96-well plate was taken, 100 μL of cell suspension was inoculated in each well, and the final cell density was 7×10 3The cells were incubated in a 5% CO2, 37°C incubator for 24 hours. The experimental groups were divided into a blank control group and a sample group. The sample group was added with samples 1-11 and comparative samples 1-5 and incubated for another 24 hours. The blank control group was added with 100 μL of DMEM medium. Each group had three parallel holes. The cells were incubated in a 5% CO2, 37°C incubator for 24 hours. 10 μL of CCK-8 working solution was added to each hole. The holes were incubated in an incubator for 0.5 hours. The absorbance value (OD) at 450 nm was measured by an enzyme-labeled instrument. The cell viability was calculated based on the blank control group. Cell viability % = (sample group OD) / (blank control group OD) x 100%.

[0071] The results are shown in Table 2.

[0072] Table 2 Cell viability of the hair papilla cells in each experimental group

[0073]

[0074]

[0075] 2. Test of the efficacy of promoting the metabolic capacity of hair papilla cells

[0076] The experimental steps are as follows: KNU201 immortalized hair papilla cells were inoculated in a 96-well plate. The cell density was adjusted to 7 x 10 4 cells / mL. A standard 96-well plate was taken. 100 μL of cell suspension was inoculated in each hole to make the final cell density 7 x 10 3The cells were placed in a 5% CO2, 37°C incubator for constant temperature culture for 24 h. The experimental groups were divided into a blank control group and a sample group. The sample group was added with samples 1-11 and comparative samples 1-5 respectively and continued to be cultured for 24 h. The blank control group was only added with 100 μL of DMEM medium. Each group was provided with three parallel holes. The cells were placed in a 5% CO2, 37°C incubator for constant temperature culture for 24 h. Before use, the required amount of working solution was calculated according to the amount of 100 μL per hole. The JC-1 probe mother liquor was diluted with the JC-1 staining buffer in the kit according to a final concentration of 1 μg / mL. The Hoechst dye was diluted according to a final concentration of 1 μg / mL and was ready for use in the dark. The cell culture medium was replaced with the JC-1 probe working solution. The cells were incubated in a 37°C incubator in the dark for 30 minutes. The cells were washed twice with the JC-1 staining buffer. The JC-1 staining buffer was removed. 100 μL of DMEM medium was added to each hole. The cells were placed in a fluorescence microscope system. DAPI, Green and Texas Red channels were selected. The appropriate focusing mode, exposure time and gain were selected. The cells were photographed. The red and green fluorescence intensity values of each cell were measured. The red / green fluorescence ratio of the blank control group was taken as the benchmark. The red / green fluorescence ratio of each cell was calculated. The mitochondrial membrane potential red / green fluorescence ratio % = (red fluorescence intensity of each cell in the sample group / green fluorescence intensity of each cell in the sample group) / (red fluorescence intensity of each cell in the blank control group / green fluorescence intensity of each cell in the blank control group) x 100%.

[0077] The results are shown in Table 3.

[0078] Table 3: Mitochondrial membrane potential red / green fluorescence ratio % of the hair papilla cells in each experimental group

[0079] Experimental group Mitochondrial membrane potential red / green fluorescence ratio % Blank control group 100.00％±12.27％ Sample group 1 132.89％±4.08％ Sample group 2 121.61％±4.72％ Sample group 3 109.51％±7.64％ Sample group 4 105.10％±6.57％ Sample group 5 101.38％±8.54％ Sample group 6 98.41％±7.53％ Sample group 7 98.43％±9.68％ Sample group 8 103.07％±6.59％ Sample group 9 107.13％±10.18％ Sample group 10 104.57％±7.75％ Sample group 11 127.24％±10.35％ Comparative sample group 1 90.36％±8.24％ Comparative sample group 2 86.91％±7.10％ Comparative sample group 3 91.06％±9.25％ Comparative sample group 4 92.72％±8.65％ Comparative sample group 5 95.56％±8.98％

[0080] The cell viability results of the hair papilla cells KNU201 in Table 2 show that the sample groups 1-11 all have hair papilla cell viability up-regulation effects and can promote the metabolic function of the hair papilla cells. The effect of the sample group 1 is the best. The effect of the sample group 1 is also obviously better than that of the comparative sample group 1-5, which indicates that the selected components in the present application can exert a synergistic effect.

[0081] The mitochondrial membrane potential results of the hair papilla cells KNU201 in Table 3 show that the sample groups 1-11 all have hair papilla cell mitochondrial function up-regulation effects and can enhance the metabolic function of the hair papilla cells. The effect of the sample group 1 is the best. The effect of the sample group 1 is also obviously better than that of the comparative sample group 1-5, which indicates that the selected components in the present application can exert a synergistic effect.

[0082] Application Example 1

[0083] The composition of Example 1 was used to prepare a shampoo. The specific formula is shown in Table 4.

[0084] Table 4

[0085]

[0086]

[0087] Application Example 2

[0088] The composition of Example 10 was used to prepare a shampoo, the specific formulation of which is shown in Table 5.

[0089] Table 5

[0090]

[0091]

[0092] Application Example 3

[0093] The composition of Example 11 was used to prepare a shampoo, the specific formulation of which is shown in Table 6.

[0094] Table 6

[0095]

[0096] The shampoos of the above-mentioned Application Examples 1-3 can be prepared according to conventional methods.

[0097] The shampoos of Application Examples 1-3, due to containing the composition of the present application, have the effects of the composition of the present application accordingly.

[0098] The above describes the preferred embodiments of the present application in detail, but the present application is not limited to the above-mentioned embodiments, and those skilled in the art can make various equivalent modifications or replacements without departing from the spirit of the present application, and these equivalent modifications or replacements are all included in the scope defined by the claims of the present application.

Claims

1. A composition characterized in that, The composition comprises the following components: biota leaf extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4.

2. The composition of claim 1, wherein, The mass ratio of the biota leaf extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4 is 0.1:(0.01-0.1):(0.01-0.1):(0.000001-0.0001):(0.000001-0.0001).

3. Process for the preparation of a composition according to claim 1 or 2, characterised in that, The method comprises the following steps: The components are mixed to obtain the composition.

4. Use of the composition of claim 1 or 2 in the preparation of a product with the efficacy of preventing hair loss.

5. Use according to claim 4, characterized in that, The product comprises a washing and protecting product or an external skin preparation.

6. Use according to claim 4, characterized in that, The efficacy of preventing hair loss comprises promoting the cell viability of hair papilla cells and / or promoting the cell metabolic function of hair papilla cells.

7. A cleansing product characterized by, The washing and protecting product comprises the composition of claim 1 or 2.

8. The wash and care product according to Claim 7, characterized in that, The mass content of the composition in the washing and protecting product is 0.01%-1%.

9. The wash and care product according to Claim 7, characterized in that, The mass content of the biota leaf extract in the washing and protecting product is 0.0001%-0.1%, the mass content of the trehalose in the washing and protecting product is 0.01%-0.1%, the mass content of the betaine in the washing and protecting product is 0.01%-0.1%, the mass content of the acetyl tetrapeptide-3 in the washing and protecting product is 0.000001%-0.0001%, and the mass content of the myristoyl pentapeptide-4 in the washing and protecting product is 0.000001%-0.0001%.

10. The wash and care product according to Claim 7, characterized in that, The washing and protecting product comprises shampoo, hair care spray, hair oil, hair conditioner or hair mask.

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