Application of Qamgur puree in preparation of preparation for dispelling effects of alcohol and protecting liver

Abstract

The invention discloses brassica rapa primary pulp prepared by adopting a specific process and application of the brassica rapa primary pulp in preparation of a preparation for dispelling effects of alcohol and protecting liver. The preparation method comprises the following steps: cleaning with specific parameters, sorting, crushing, squeezing, filtering, percolating, carrying out double-effect concentration, filling and sterilizing to obtain the Brassica rapa raw stock healthy beverage with the effects of dispelling the effects of alcohol and protecting the liver. The prepared healthy beverage adopts pure plant brassica rapa as a raw material, the production process is simple, convenient and feasible, the beverage is convenient to carry and use, green and safe, and the puree product not only contains various nutritional ingredients, but also has the effects of dispelling the effects of alcohol and protecting the liver. Through experiments, the brassica rapa protoplasm provided by the invention can effectively enhance the activity of ethanol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), including enhancing the activity of key enzymes ADH and ALDH in an ethanol metabolism path, accelerating the in-vivo ethanol metabolism speed and relieving liver injury caused by drunkenness, has the effect of protecting the body, and meanwhile, remarkably improves the liver fat accumulation condition of the body, so that the liver injury caused by drunkenness is relieved. The Brassica rapa primary pulp product provides a new choice for dispelling the effects of alcohol and protecting the liver, and has a wide market application prospect.

Description

Technical Field

[0001] This invention belongs to the technical field of health beverages, specifically relating to the technical field of processing and application of Chaga mushroom, and more specifically relating to the technical field of using Chaga mushroom puree in the preparation of hangover relief and liver protection preparations. Background Technology

[0002] The World Health Organization (WHO) states that alcohol poisoning is one of the world's major public health problems. Most of the ethanol ingested through alcohol is metabolized by the liver, damaging liver cell structure and disrupting normal cell metabolism, producing cytotoxic metabolites. Alcoholic Liver Disease (ALD) is a significant cause of morbidity and mortality from liver-related diseases worldwide. ALD refers to toxic pathological damage to the liver caused by short-term heavy drinking or long-term alcoholism. In its early stages, the main pathological manifestation is hepatocyte steatosis, which gradually progresses to alcoholic hepatitis, eventually leading to liver fibrosis, alcoholic cirrhosis, and in severe cases, liver cancer, seriously threatening human health. Although research on the mechanisms of alcohol metabolism and the pathology of liver damage has made some progress, clinical interventions for ALD still have limitations. Modern medicine mostly adopts methods such as alcohol abstinence and nutritional support. However, the efficacy of existing drugs is insufficient; in severe cases, glucocorticoids and metoprolol can be added for intervention, but all of these have significant side effects. Traditional Chinese medicine records alcoholic liver damage under the categories of "alcohol jaundice," "alcohol disease," and "alcohol accumulation." The Compendium of Materia Medica states that "a small amount of alcohol can harmonize qi and blood, while excessive drinking can kill instantly," suggesting that moderate alcohol consumption can relax muscles, dispel cold, and regulate qi and blood, while excessive drinking damages the liver, spleen, and other internal organs, potentially leading to liver damage over time. Based on the pathogenesis, clinical treatment for this condition often focuses on "treating the spleen," combining strengthening the body's resistance with eliminating pathogenic factors, and adding medications to detoxify the liver and aid digestion. Therefore, exploring natural or synthetic active ingredients and developing efficient and safe strategies for detoxifying the liver and relieving alcohol poisoning has become an important direction in current pharmacological and nutritional research.

[0003] In recent years, zebrafish (*Danio rerio*) has emerged as a novel vertebrate model organism, demonstrating unique advantages in liver disease research. Its transparent embryos, short developmental cycle, and highly conserved genes (over 70% homology with humans), coupled with its liver tissue structure highly similar to that of mammals, provide an ideal platform for high-throughput drug screening and pathological mechanism analysis. Furthermore, zebrafish larvae can be directly exposed to ethanol and drug molecules in the aquatic environment, facilitating real-time observation of the dynamic process of liver injury and repair effects, thus overcoming the limitations of traditional mammalian models in terms of real-time imaging and operational convenience. Studies have shown that the zebrafish ethanol metabolism pathway is highly similar to that of humans, and the expression patterns of key enzymes such as alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), and cytochrome P450 2E1 (CYP2E1) can effectively mimic the human alcohol metabolism process. It is noteworthy that traditional medicinal and edible plant resources have attracted significant attention in the field of hangover relief and liver protection. Chamaegu (Brassicarapa L.), also known as turnip, is a cruciferous plant rich in glucosinolates, isothiocyanates, polyphenols, and vitamins. Its pulp is often used in traditional medicine to relieve alcohol poisoning and protect liver function.

[0004] Chamaegu is a common vegetable consumed by various ethnic groups in Xinjiang, China, and is widely cultivated in the region. Its tubers are eaten cooked or used to make pickled vegetables. Studies have shown that Chamaegu contains vitamins, proteins, carbohydrates, crude fiber, linoleic acid, flavonoids, and various amino acids. The Ming Dynasty medical text *Compendium of Materia Medica* records that Chamaegu's roots and leaves are "bitter, warm, and non-toxic, benefiting the five internal organs." Chamaegu polysaccharides possess anti-tumor, antioxidant, anti-inflammatory, hepatoprotective, and immune-enhancing properties. Feng Qi analyzed the hepatoprotective effects of Chamaegu extract using an in vitro model of alcoholic liver injury induced by ethanol in HepG2 cells, finding that it significantly inhibited ethanol-induced proliferative toxicity. Kong Hanrui's research found that BRCpe combined with cisplatin synergistically inhibited the proliferation of HepG2 cells and promoted HepG2 cell apoptosis. The mechanism may be that the two synergistically activate the mitochondrial signaling pathway, enhancing the sensitivity of cisplatin to tumor cells, providing a scientific basis and theoretical foundation for using Chamaegu polysaccharides as an adjuvant drug for treating liver cancer or as a chemosensitizer for cisplatin. Therefore, there is an urgent need to develop a natural product that can alleviate alcoholic liver damage without toxic side effects, and to verify its functional properties. Currently, the main production method is to process Chamaegu (a type of medicinal herb) into liquid beverages, solid beverages, tablets, and sauces. Extracts from Chamaegu roots have been made into "Chamaegu Honey Paste" and "Chamaegu Capsules" for use in adjuvant immunotherapy of tumors. Domestically, a series of related oral liquids, capsules, honey pastes, and essential oils have also been developed, and acute toxicity tests on Chamaegu Honey Paste have been completed. Summary of the Invention

[0005] Based on the above, this application aims to provide a product of *Chamaegu* (a type of wild herb) extract and its use in the preparation of hangover remedies and liver-protecting agents. Addressing the technical challenges of the incompletely elucidated target points and multi-dimensional regulation of the active ingredients in this product's hangover remedy and liver-protecting effects, which limit further product development and application, this application uses zebrafish as a model animal. Through establishing a zebrafish excitation-phase intoxication experiment, evaluating liver-protecting efficacy, and monitoring changes in alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) levels, the hangover remedy and liver-protecting effects of *Chamaegu* extract are systematically evaluated. The related technical solution not only provides a scientific basis for the medicinal value of *Chamaegu* extract but also lays a theoretical foundation for developing ALD prevention and treatment strategies based on natural products and for the standardized application of the zebrafish model. This extract product offers a new option for hangover remedy and liver protection and has broad market application prospects.

[0006] To achieve this technical objective, the present invention adopts the following technical solution: On the one hand, this application provides the use of Chamaegu raw pulp in the preparation of hangover relief and liver protection preparations, wherein the concentration of Chamaegu raw pulp is not higher than 3.12 μL / mL.

[0007] On the other hand, the chamaegu pulp is prepared by the following steps: (1) Remove the surface soil from the obtained Chamaegu, wash it again to ensure the cleanliness of the sample, and manually sort it to remove bad or inferior fruits such as those with mold or insect infestation. (2) Crush the cleaned Chamaegu into small pieces of about 2mm x 2mm; (3) Squeeze the crushed material to extract the juice and place it in a 2000L storage tank. The juice is cloudy white and semi-transparent. (4) The obtained pressed residue is percolated at a material ratio of about 1:1, with hot water temperature greater than 70℃, and mixed with fruit residue at about 40-50℃; (5) Combine the juice sample with the fruit residue extracted by hot water, and perform a second extraction by percolation to collect the percolate; (6) The obtained percolate was concentrated at -0.02~-0.07MPa and 80-90℃ for about 0.5-2 h. The sugar content of the original pulp was monitored by a sugar content analyzer. The final sugar content was about 14-22 Brix. (7) Keep the bottled Chamaegu concentrated solution at 70-90℃ for 15 min-1.5 h to effectively inactivate the microorganisms in the Chamaegu juice; (8) The concentrated juice of Chamaegu is placed in a plastic bucket, then transferred to the filling line and filled into pre-cleaned and sterilized glass bottles, 30 mL per bottle, to obtain Chamaegu raw juice.

[0008] Preferably, in step (2), a peeling device can be added after cleaning to remove the outer skin and the periphery of the stem of the Chamaegu.

[0009] Preferably, in step (4), the slag is soaked in hot water for 3-4 hours; Preferably, the obtained percolate is concentrated at -0.04~-0.07 MPa and 80℃ for about 1 hour, and the sugar content of the original pulp is monitored by a sugar content analyzer. The final sugar content is about 20 Brix. Preferably, the bottled Chamaegu concentrate is kept at 70°C for 15 minutes.

[0010] Furthermore, this application provides the use of the aforementioned Chamaegu raw pulp in the preparation of a hangover remedy and liver-protecting agent, wherein the use is for improving the hangover remedy and liver-protecting function of the subject, or for use in the preparation of a product for improving the hangover remedy and liver-protecting function of the subject.

[0011] The improvement of the target's hangover relief and liver protection functions includes hangover relief during the excitement period, liver protection, increasing ADH and ALDH levels, and liver tissue recovery. Furthermore, this application provides the use of the aforementioned Chamaegu raw pulp in the preparation of hangover relief and liver protection preparations, wherein the use is that the Chamaegu raw pulp, or the Chamaegu raw pulp together with edible excipients, is prepared into a food product. The stated uses refer to the product being taken orally, subcutaneously, intramuscularly, or intraperitoneally. By implementing the technical solution of the present invention, the following beneficial effects can be achieved: This invention discloses a Chamaegu (a type of wild herb) puree prepared using a specific process and its use in the preparation of hangover remedies and liver-protecting agents. A health beverage containing Chamaegu puree with hangover relieving and liver-protecting effects is obtained through a combination of washing, sorting, crushing, pressing, filtering, percolation, double-effect concentration, bottling, and sterilization using specific parameters. The health beverage prepared by this invention uses pure Chamaegu as raw material. The production process is simple, easy to carry and use, green, and safe. This puree product not only contains various nutrients but also has hangover relieving and liver-protecting effects. Through experiments, the Chamaegu puree provided in this application can effectively enhance the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), including enhancing the activity of key enzymes in the ethanol metabolism pathway, ADH and ALDH, accelerating the rate of ethanol metabolism in the body, and reducing liver damage caused by drunkenness, thus protecting the body. Simultaneously, it significantly improves the accumulation of fat in the liver. This Chamaegu puree product provides a new option for hangover relieving and liver protection and has broad market application prospects. Attached Figure Description

[0012] Figure 1 The image shown is a typical behavior diagram of zebrafish after sample treatment, and the detection results are displayed. Figure 2 The image shows the results of the total movement distance of zebrafish after sample processing. Figure 3The image shows the typical detection results of fat staining intensity in zebrafish livers after sample processing. Figure 4 The image shows the results of the zebrafish liver fat staining intensity test after sample processing; Figure 5 The results of ADH content detection in zebrafish after sample treatment are shown. Figure 6 The results of ALDH content detection in zebrafish after sample treatment are shown. Figure 7 The image shown is a typical image of a zebrafish liver tissue pathological section after sample processing, and the results of the detection are shown. Detailed Implementation The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0013] Unless otherwise specified, the technical means used in the following implementation examples are conventional means well known to those skilled in the art.

[0014] Example 1: A type of Chamaegu raw pulp (1) Remove the surface soil from the obtained Chamaegu, wash it again to ensure the cleanliness of the sample, and manually sort it to remove bad or inferior fruits such as those with mold or insect infestation. (2) Crush the cleaned Chamaegu into small pieces of about 2mm x 2mm; (3) Squeeze the crushed material to extract the juice and place it in a 2000L storage tank. The juice is cloudy white and semi-transparent. (4) The obtained pressed residue is percolated at a material ratio of about 1:1, with hot water temperature greater than 70℃, and mixed with fruit residue at about 40-50℃; (5) Combine the juice sample with the fruit residue extracted by hot water extraction, and perform a second extraction by percolation to collect the percolate; (6) The obtained percolate was concentrated at -0.02~-0.07MPa and 80-90℃ for about 0.5-2 h. The sugar content of the original pulp was monitored by a sugar content analyzer. The final sugar content was about 14-22 Brix. (7) Keep the bottled Chamaegu concentrated solution at 70-90℃ for 15 min-1.5 h to effectively inactivate the microorganisms in the Chamaegu juice; (8) The concentrated juice of Chamaegu is placed in a plastic bucket, then transferred to the filling line and filled into pre-cleaned and sterilized glass bottles, 30 mL per bottle, to obtain Chamaegu raw juice.

[0015] Example 2: A type of Chamaegu raw pulp The obtained Chamaegu fruit was cleaned of surface dirt and washed again to ensure sample cleanliness. It was then manually sorted, removing moldy, insect-damaged, or other defective fruit. The cleaned Chamaegu fruit was crushed into approximately 2mm x 2mm particles. The juice was extracted from the crushed material and placed in a 2000L storage tank; the juice was a cloudy, white, semi-transparent liquid. The pressed residue was percolated with the fruit pomace at a material ratio of approximately 1:1, using hot water at approximately 90℃ and a temperature of approximately 40-50℃. The extracted juice was combined with the hot water-extracted pomace and subjected to a second extraction via percolation, collecting the percolate. The percolate was concentrated at -0.02~-0.03MPa and 90℃ for approximately 2 hours. The final sugar content was measured using a saccharimeter, reaching approximately 14 Brix. The bottled Chamaegu concentrated solution was then kept at 90℃ for 1.5 hours. h, to effectively inactivate microorganisms in Chamaegu juice; Chamaegu concentrated juice is placed in plastic buckets, then transferred to the filling line, and filled into pre-cleaned and sterilized glass bottles, 30 mL per bottle, to obtain Chamaegu raw pulp.

[0016] Example 3: A kind of Chamaegu raw pulp The obtained Chamaegu fruit was cleaned of surface dirt and washed again to ensure sample cleanliness. It was then manually sorted, removing moldy, insect-damaged, or other damaged or inferior fruit. After washing, a peeling device was added to remove the outer skin and the periphery of the stem, crushing the fruit into approximately 2mm x 2mm particles. The crushed material was squeezed to extract juice, which was placed in a 2000L storage tank. The juice was a cloudy, white, semi-transparent liquid. The resulting pressed residue was soaked in hot water at approximately 70℃ for 3-4 hours at a material-to-water ratio of approximately 1:1, and then mixed with the fruit pomace and heated to approximately 40-50℃. Percolation was performed; the juice sample was combined with the pomace extracted by hot water extraction, and a second extraction was performed by percolation to collect the percolate; the obtained percolate was concentrated at -0.04~-0.07 MPa and 80℃ for about 1 hour, and the sugar content of the original pulp was monitored using a saccharimeter, with the final sugar content being about 20 Brix; the bottled Chamaegu concentrated liquid was kept at 70℃ for 15 minutes to effectively inactivate the microorganisms in the Chamaegu juice; the Chamaegu concentrated liquid was placed in plastic barrels, then transferred to the filling line, and filled into pre-cleaned and sterilized glass bottles, 30 mL per bottle, to obtain Chamaegu original pulp.

[0017] Example 4: A Chamaegu Original Pulp The obtained Chamaegu fruit was cleaned of surface dirt and washed again to ensure sample cleanliness. It was then manually sorted, removing moldy, insect-damaged, or other damaged or inferior fruit. After washing, a peeling device was added to remove the outer skin and the periphery of the stem, crushing the fruit into approximately 2mm x 2mm particles. The crushed material was squeezed to extract juice, which was placed in a 2000L storage tank. The juice was a cloudy, white, semi-transparent liquid. The resulting pressed residue was soaked in hot water at approximately 70℃ for 3-4 hours at a material-to-water ratio of approximately 1:1, and then mixed with the fruit pomace and heated to approximately 40-50℃. Percolation was performed; the juice sample was combined with the pomace extracted by hot water extraction, and a second extraction was performed by percolation to collect the percolate; the obtained percolate was concentrated at -0.04~-0.07 MPa and 90℃ for about 1 hour, and the sugar content of the original pulp was monitored using a saccharimeter, with the final sugar content being about 22 Brix; the bottled Chamaegu concentrated liquid was kept at 70℃ for 30 minutes to effectively inactivate the microorganisms in the Chamaegu juice; the Chamaegu concentrated liquid was placed in plastic barrels, then transferred to the filling line, and filled into pre-cleaned and sterilized glass bottles, 30 mL per bottle, to obtain Chamaegu original pulp.

[0018] Example 5: Comparison of the activity of Chamaegu pulp Based on the *Chamaegu* raw pulp prepared in Examples 2 to 4 above, the total phenols and uric acid inhibition activity (xanthine oxidase inhibition rate) were determined according to the relevant methods in "Analysis of Flavonoid, Phenolic Acid Content and Antioxidant Capacity of Tartary Buckwheat Beverages" and "Construction of Zebrafish Hyperuricemia Model and Screening of Uric Acid-Lowering Functional Food and Medicinal Materials". The results are shown in Table 1 below. Table 1: Comparison of the activity of Chamaegu raw pulp

[0019] Example 6: Alcohol Detoxification and Liver Protection Test Animal experiments were conducted using the Chamaegu raw pulp prepared in Example 3 to demonstrate that the present invention has the effect of relieving hangovers and protecting the liver. All experimental zebrafish were raised in aquarium water at 28 ℃ (water quality: 200 mg of readily soluble sea salt added per 1 L of reverse osmosis water, conductivity 450~550 μS / cm; pH 6.5~8.5; hardness 50~100 mg / L CaCO3), bred and provided by the fish farming center of Hangzhou Huante Biotechnology Co., Ltd., with experimental animal use license number: SYXK (Zhejiang) 2022-0004, and husbandry management in accordance with the requirements of international AAALAC certification (certification number: 001458), IACUC ethics review number: IACUC-2025-11612-01.

[0020] Experiment 1: Determination of Maximum Detectable Concentration (MTC) Wild-type AB strain zebrafish, 5 days post-fertilization (5 dpf), were randomly selected and placed in 6-well plates, with 30 zebrafish treated in each well (experimental group). Samples were administered in water (concentrations shown in Table 1). A normal control group and a model control group were also included, with a volume of 3 mL per well. Except for the normal control group, all other experimental groups were treated with anhydrous ethanol to establish a zebrafish alcoholic fatty liver model. After treatment at 28℃ for 1 day, the MTC of the samples in the model zebrafish was measured. The experimental results are shown in Table 2. Table 2: Results of the experiment to explore the concentration of samples for hangover relief and liver protection (n = 30)

[0021] As shown in Table 2, compared with the control group, it can be clearly seen that under the experimental conditions of this invention, the MTC of the liver-protecting and hangover-relieving effects of Chamaegu raw juice is 3.12 μL / mL.

[0022] Example 7: Evaluation of the effects of alcohol detoxification and liver protection (efficacy in detoxification during the excitement phase) Wild-type AB strain zebrafish with a 5-day pf (dpf) count were randomly selected and placed in 6-well plates, with 30 zebrafish treated in each well (experimental group). Samples were administered in water (concentrations shown in Table 2), with a positive control of RU21 at a concentration of 100 μg / mL. A normal control group and a model control group were also included, with a volume of 3 mL per well. After treatment at 28℃ for one day, 10 zebrafish from each group were randomly selected and placed in 96-well plates, one zebrafish per well, with a volume of 200 μL per well. Except for the normal control group, the other experimental groups were administered anhydrous ethanol to establish a zebrafish alcohol intoxication model during the excitation phase. The total movement distance of the zebrafish was measured using a behavioral analyzer, and the statistical analysis results of this index were used to evaluate the alcohol-relieving and liver-protective effects (excitation-phase alcohol detoxification) of the samples. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS software. p A value <0.05 indicates that the difference is statistically significant. The experimental results are shown in Table 3.

[0023] Table 3: Experimental results evaluating the efficacy of the samples in relieving hangovers and protecting the liver (relieving hangovers during the excitement phase) (n = 10)

[0024] Compared with the model control group, *p<0.05, **p<0.01 Under the conditions of this experiment, Chamaegu raw liquor has an effect of relieving hangovers during the excitatory phase. See appendix. Figure 1 and attached Figure 2 Appendix Figure 1 The black line represents the slow-speed running distance, the green line represents the medium-speed running distance, and the red line represents the fast-speed running distance. Figure 2 Compared with the model control group, *p<0.05, **p<0.01.

[0025] Example 8: Evaluation of the effects of alcohol detoxification and liver protection (liver protection effect) Wild-type AB strain zebrafish with a 5 dpf growth rate were randomly selected and placed in 6-well plates, with 30 zebrafish treated in each well (experimental group). Samples were administered in water (concentrations shown in Table 3), with a positive control of RU21 at a concentration of 100 µg / mL. A normal control group and a model control group were also included, with a volume of 3 mL per well. Except for the normal control group, all other experimental groups were treated with anhydrous ethanol to establish a zebrafish alcoholic fatty liver model. After treatment at 28℃ for 1 day, whole-body fat was stained with Oil Red O. After destaining and bleaching, 10 zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software to analyze the intensity of liver fat staining. The statistical analysis results of this index were used to evaluate the alcohol-relieving and liver-protective efficacy of the samples. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS software. p A value <0.05 indicates a statistically significant difference. The results are shown in Table 4.

[0026] Table 4: Experimental results evaluating the hangover relief and liver protection efficacy of the samples (n = 10)

[0027] Compared with the model control group, **p<0.01, ***p<0.001 Chamaegu extract has hangover-relieving and liver-protecting effects, specifically by reducing the intensity of fat staining in the liver. (See appendix.) Figure 3 And attached diagram Figure 4 Appendix Figure 3 The yellow dashed box indicates the analysis area. Figure 4 Compared with the model control group, **p<0.01, ***p<0.001 Example 9: Evaluation of the effects of alcohol detoxification and liver protection (ADH content) Wild-type AB strain zebrafish with a dpf of 5 were randomly selected and placed in 6-well plates, with 30 zebrafish treated in each well (experimental group). Samples were administered via water-soluble solution at concentrations shown in Table 5. The positive control RU21 was administered at a concentration of 100 µg / mL. A normal control group and a model control group were also included, with a volume of 3 mL per well. Except for the normal control group, all experimental groups were administered anhydrous ethanol via water-soluble solution to establish a zebrafish alcoholic fatty liver model. The experiment was conducted in triplicate. After treatment at 28℃ for 1 day, data were collected using a multi-functional microplate reader according to the Zebrafish ADH Elisa Kit instructions. The ADH content in the zebrafish was analyzed, and the statistical analysis results were used to evaluate the alcohol-relieving and liver-protective effects of the sample. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS software, and p < 0.05 indicated statistical significance. Under the experimental conditions, *Chamaegu* extract showed alcohol-relieving and liver-protective effects, specifically by increasing the ADH content in zebrafish. See Table 5 and Appendix for details. Figure 5 .

[0028] Table 5: Experimental results evaluating the efficacy of the samples in relieving hangovers and protecting the liver (ADH content) (n = 3)

[0029] Compared with the model control group, **p<0.01, ***p<0.001 Figure 5 Compared with the model control group, **p<0.01, ***p<0.001 Implementation 10: Evaluation of the effects of alcohol detoxification and liver protection (ALDH content) Wild-type AB strain zebrafish with a 5-day pf (dpf) count were randomly selected and placed in 6-well plates, with 30 zebrafish treated in each well (experimental group). Samples were administered via water-soluble solution at concentrations shown in Table 6. The positive control, RU21, was administered at a concentration of 100 µg / mL. A normal control group and a model control group were also included, with a volume of 3 mL per well. Except for the normal control group, all experimental groups were administered anhydrous ethanol via water-soluble solution to establish a zebrafish alcoholic fatty liver model. The experiment was conducted in triplicate. After treatment at 28℃ for one day, data were collected using a multi-functional microplate reader according to the Zebrafish ALDH Elisa Kit instructions. The ALDH content in the zebrafish was analyzed, and the statistical analysis results were used to evaluate the alcohol-relieving and liver-protective effects of the sample. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS software, and p < 0.05 indicated statistical significance. Under the experimental conditions, Zebrafish extract showed alcohol-relieving and liver-protective effects, specifically by increasing the ALDH content in zebrafish. See Table 6 and Appendix for details. Figure 6 .

[0030] Table 6: Experimental results evaluating the efficacy of the samples in relieving hangovers and protecting the liver (ALDH content) (n = 3)

[0031] Compared with the model control group, *p<0.05, **p<0.01 Figure 6 Compared with the model control group, *p<0.05, **p<0.01 Example 11: Evaluation of the efficacy of alcohol detoxification and liver protection (pathological sections) Wild-type AB strain zebrafish with a 5 dpf growth rate were randomly selected and placed in 6-well plates, with 30 zebrafish treated in each well (experimental group). The samples were administered water-soluble solutions at the concentrations specified in the appendix. Figure 7 As shown, the positive control RU21 was at a concentration of 100 µg / mL. A normal control group and a model control group were also set up, with a volume of 3 mL per well. Except for the normal control group, all other experimental groups were treated with anhydrous ethanol to establish a zebrafish alcoholic fatty liver model. After treatment at 28℃ for 1 day, the zebrafish were fixed with 4% histocellular fixative. Following a series of steps including dehydration, embedding, sectioning, and staining, the zebrafish underwent histopathological H&E staining analysis. The impact of the samples on the zebrafish liver tissue structure was evaluated through histopathological analysis.

[0032] Under the above experimental conditions, the liver cells of zebrafish in the normal control group were tightly arranged, with intact cell structure, clear outline, regular nucleus size and shape, and no fatty vacuoles were observed. In the model control group, the liver cells were disordered, with incomplete cell structure, significantly increased internuclear distance, and obvious fatty vacuoles, as indicated by the black arrows, indicating successful model establishment. Compared with the model control group, the positive control group (RU21 100 μg / mL) showed a significant reduction in fatty vacuoles and a smaller internuclear distance in liver tissue, indicating that RU21 has an ameliorative effect on alcoholic fatty liver. The groups treated with Chamaegu extract at concentrations of 0.781, 1.56, and 3.12 μL / mL also showed a significant reduction in fatty vacuoles and a smaller internuclear distance in liver tissue, indicating that Chamaegu extract has an ameliorative effect on alcoholic fatty liver. In conclusion, Chamaegu extract has a detoxifying and liver-protective effect, specifically manifested in liver tissue recovery. (See attached figure) Figure 7 Appendix Figure 7 The red dashed box in the middle represents the liver area being analyzed, and the black arrow points to the fatty vacuoles.

[0033] In summary, the Chamaegu raw pulp prepared by the technical solution provided in this application and its use in the preparation of hangover relief and liver protection preparations have hangover relief and liver protection effects and uric acid reduction effects. The hangover relief and liver protection effects are specifically manifested in the effects of relieving hangovers during the excitement period, liver protection, increasing the ADH and ALDH content in zebrafish, and liver tissue recovery.

[0034] The above embodiments are only for illustrating the technical concept and features of the present invention in a specific scenario. Their purpose is to enable those who need this technology to understand the content of the present invention and implement it. They do not limit the scope of protection of the present invention. All equivalent changes or modifications made according to the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. The use of Chamaegu raw pulp in the preparation of hangover remedies and liver-protecting agents, characterized in that, The concentration of the raw chamaegu pulp is no higher than 3.12 μL / mL.

2. The use of the Chamaegu raw material as described in claim 1 in the preparation of hangover relief and liver protection preparations, characterized in that, The stated use is for improving the liver function of a subject in relieving hangovers, or for use in preparing a product for improving the liver function of a subject in relieving hangovers.

3. The use of Chamaegu raw pulp as described in claim 2 in the preparation of hangover remedies and liver-protecting agents, characterized in that, The improvement of the target's hangover relief and liver protection functions includes hangover relief during the excitement period, liver protection, increasing ADH and ALDH levels, and liver tissue recovery.

4. The use of the Chamaegu raw material as described in claim 1 in the preparation of hangover relief and liver protection preparations, characterized in that, The intended use is for making Chamaegu puree, or for preparing food products together with edible additives.

5. The use of the Chamaegu raw material as described in claim 4 in the preparation of a hangover remedy and liver-protecting agent, characterized in that, The stated purpose is for the product to be taken orally, subcutaneously, intramuscularly, or intraperitoneally.

6. The use of the Chamaegu raw material as described in claim 1 in the preparation of hangover relief and liver protection preparations, characterized in that, The aforementioned Chamaegu raw pulp is prepared using the following steps: (1) Remove the surface soil from the obtained Chamaegu, wash it again to ensure the cleanliness of the sample, and manually sort it to remove bad or inferior fruits such as those with mold or insect infestation. (2) Crush the cleaned Chamaegu into small pieces of about 2mm × 2mm; (3) Squeeze the crushed material to extract the juice and place it in a 2000L storage tank. The juice is cloudy white and semi-transparent. (4) The obtained pressed residue is percolated at a material ratio of about 1:1, with hot water temperature greater than 70℃, and mixed with fruit residue at about 40-50℃; (5) Combine the juice sample with the fruit residue extracted by hot water, and perform a second extraction by percolation to collect the percolate; (6) The obtained percolate was concentrated at -0.02-0.07 MPa and 80-90℃ for about 0.5-2 h. The sugar content of the original pulp was monitored by a sugar content analyzer. The final sugar content was about 14-22 Brix. (7) Keep the bottled Chamaegu concentrated solution at 70-90℃ for 10 min-1.5 h to effectively inactivate the microorganisms in the Chamaegu juice; (8) The concentrated juice of Chamaegu is placed in a plastic bucket, then transferred to the filling line and filled into pre-cleaned and sterilized glass bottles, 30 mL per bottle, to obtain Chamaegu raw juice.

7. The use of Chamaegu raw pulp as described in claim 1 in the preparation of hangover remedies and liver-protecting agents, characterized in that, In the preparation steps of Chamaegu pulp, step (2) can also involve adding a peeling device after washing to remove the outer skin and the periphery of the stem of Chamaegu.

8. The use of the Chamaegu raw material as described in claim 1 in the preparation of a hangover remedy and liver-protecting agent, characterized in that, In the preparation steps of the Chamagu raw pulp, the slag in step (4) is soaked in hot water for 3-4 hours.

9. The use of the Chamaegu raw material as described in claim 1 in the preparation of a hangover remedy and liver-protecting agent, characterized in that, In the preparation steps of Chamaegu pulp, in step (6), the obtained percolate is concentrated at -0.04~-0.07MPa and 80℃ for about 1 h, and the pulp sugar content is monitored by a sugar content meter. The final sugar content is about 20 Brix.

10. The use of the Chamaegu raw pulp as described in claim 1 in the preparation of a hangover remedy and liver-protecting agent, characterized in that, In the preparation steps of Chamaegu raw pulp, the Chamaegu concentrated liquid bottled in step (7) is kept at 70°C for 15 minutes.

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