A multi-channel microfluidic chip active valve

Abstract

The application discloses a kind of multi-channel microfluidic chip active valve, including bearing layer and flow control layer.Bearing layer extends along first direction;Flow control layer is arranged on bearing layer, and flow control layer is spaced apart and arranged with multiple groups of flow control components along the first direction on flow control layer, any one group of flow control components includes through portion for fluid to pass through and blocking portion for blocking fluid, the face of flow control component in flow control layer is first side face, microfluidic chip has second sealing surface that is combined with the first sealing surface, fluid inlet and outlet are arranged on the second sealing surface, and the first sealing surface is combined with the second sealing surface;Flow control layer is flexible material layer, and bearing layer and flow control layer form integrated valve body structure, driving force is applied to valve body structure, and valve body structure is driven to reciprocate along the first direction, so that through portion and blocking portion are switched at the alignment position of fluid inlet and outlet on second sealing surface.It can ensure high integration and miniaturization while realizing parallel control of multi-channel fluid.

Description

Technical Field

[0001] This invention relates to the field of microfluidics, and more particularly to a multi-channel microfluidic chip active valve. Background Technology

[0002] Microfluidic chips integrate basic operations such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes onto a small chip, automating the entire analysis process. Microfluidic technology has advantages such as low reagent consumption, fast analysis speed, and high integration. Microvalves are key actuators that control the flow and direction of fluid within the channels of a microfluidic chip.

[0003] Currently, the valve technologies used in microfluidic chips can be mainly divided into external solenoid valves, internally activated diaphragm valves, and rotary valves. Among them, external solenoid valves are driven by electromagnetic force, offering fast response speed and high control precision; however, these valves usually require a certain physical space between valves, limiting the integration and miniaturization of microfluidic chips. Furthermore, solenoid valves generate Joule heat during operation, which is detrimental to the detection of temperature-sensitive biochemical reaction samples.

[0004] Pneumatic diaphragm valves open and close flow channels by applying air pressure to deform an elastic diaphragm. Their advantage lies in their small dead volume, allowing them to be directly integrated into a chip. However, to achieve pneumatic control, the chip typically requires a multi-layer structure, resulting in a complex and costly manufacturing process. Furthermore, they rely on external air sources and control piping systems, leading to bulky and inaccessible testing equipment.

[0005] Rotary valves use the rotational movement of the valve core to align or misalign different flow channels, thus switching fluids. However, they can only switch one channel at a time, making them suitable for processing single samples. When faced with high-throughput, multi-index simultaneous testing requirements, they are difficult to implement multi-channel parallel fluid control, limiting their application scenarios and throughput.

[0006] In summary, existing microvalves have limitations in terms of integration and miniaturization when applied to high-throughput, multi-channel microfluidic chips. Therefore, there is an urgent need in the field for a microvalves structure that can achieve both high integration and miniaturization while enabling parallel control of multi-channel fluids. Summary of the Invention

[0007] The purpose of this invention is to provide a multi-channel microfluidic chip active valve, which solves the problem that existing microvalves have varying degrees of limitations in terms of integration and miniaturization when applied to high-throughput, multi-channel microfluidic chips.

[0008] To achieve this objective, the present invention adopts the following technical solution:

[0009] A multi-channel microfluidic chip active valve, comprising:

[0010] A support layer extending along a first direction;

[0011] A flow control layer is disposed on the support layer. Multiple sets of flow control components are arranged at intervals along a first direction on the flow control layer. Each set of flow control components includes a through part for fluid to pass through and a blocking part for blocking fluid. The surface of the flow control layer where the flow control components are disposed is a first sealing surface. The microfluidic chip has a second sealing surface that is in contact with the first sealing surface. Fluid inlet and outlet are disposed on the second sealing surface. The first sealing surface is in contact with the second sealing surface.

[0012] The flow control layer is a flexible material layer, and the bearing layer and the flow control layer form an integral valve body structure. The valve body structure reciprocates along a first direction so that the through part and the blocking part switch at the alignment position with the fluid inlet and outlet on the second sealing surface.

[0013] Optionally, the carrier layer has a groove that extends along a first direction, and the flow control layer is embedded in the groove.

[0014] Optionally, the through portion is a strip-shaped groove formed on the flow control layer along the second direction, and the blocking portion is two spaced grooves formed on the flow control layer along the second direction.

[0015] Optionally, the portion of the first sealing surface having the flow control component protrudes outward.

[0016] Optionally, it also includes a drive unit for driving the valve body structure to reciprocate along a first direction, wherein the moving distance of the valve body structure is consistent with the distance between the through part and the blocking part in the same group of flow control components.

[0017] Optionally, the supporting layer is made of a rigid adhesive material, and is selected from polycarbonate (PC), polystyrene (PS), polymethyl methacrylate (PMMA), cyclic olefin copolymer (COC), or cyclic olefin polymer (COP).

[0018] Optionally, the fluid control layer is made of a soft rubber material, such as silicone or thermoplastic elastomer.

[0019] This invention also provides a microfluidic chip, including a chip body and the aforementioned multi-channel microfluidic chip active valve. The chip body has a valve body groove for assembling the valve body structure, and the second sealing surface is the groove wall of the valve body groove.

[0020] The chip body has a sample inlet groove on its upper surface and a sample channel that communicates with the sample inlet groove inside. The sample channel is branched into multiple sub-channels, and the end of each sub-channel is connected to the second sealing surface and forms an outlet.

[0021] The chip body also has multiple sample analysis slots arranged at intervals along the first direction. Each sample analysis slot corresponds to a multiple sub-channel. Each sample analysis slot is connected to a liquid inlet channel. The inlet end of each liquid inlet channel is connected to the second sealing surface and forms a liquid inlet. The liquid inlet and the liquid outlet are arranged at intervals along the second direction.

[0022] Each set of flow control components corresponds to a set of inlet and outlet ports, respectively aligning the through section or the blocking section with the inlet and outlet ports to achieve on / off control between the sub-channel and the inlet channel.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] This invention provides a multi-channel microfluidic chip active valve. By integrating multiple spaced valve groups into the valve body structure, and controlling all flow control components to switch synchronously through unidirectional reciprocating movement, it achieves parallel control of simultaneously opening or closing multiple fluid channels. This effectively overcomes the shortcomings of single-channel switching and greatly improves the efficiency of high-throughput detection. Furthermore, the multi-channel microfluidic chip active valve consists only of a carrier layer and a flow control layer, eliminating the need for multi-layered structures and complex pneumatic control systems required by pneumatic diaphragm valves, and the need for assembling multiple independent valve bodies as with solenoid valves. The simplified integrated structure significantly reduces the manufacturing and assembly costs of the valve body structure, effectively improving the portability and integration of the microfluidic chip active valve. In addition, the soft rubber material used in the flow control layer, through its elastic deformation capability, allows the flow control layer to fit tightly against the chip in both blocked and open states, effectively sealing the flow channel interface, ensuring valve sealing, preventing fluid leakage and cross-contamination, and improving the accuracy and reliability of detection results. Therefore, the multi-channel microfluidic chip active valve provided in this embodiment of the invention can achieve parallel control of multi-channel fluids while ensuring high integration and miniaturization. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] The structures, proportions, sizes, etc., shown in the accompanying drawings of this specification are only for the purpose of assisting those skilled in the art in understanding and reading the content disclosed in the specification, and are not intended to limit the conditions under which the present invention can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in the proportions, or adjustments to the size, without affecting the effects and objectives that the present invention can produce, should still fall within the scope of the technical content disclosed in the present invention.

[0027] Figure 1 This is a schematic diagram of the structure of a multi-channel microfluidic chip active valve.

[0028] Figure 2 This is a schematic diagram of the valve body structure.

[0029] Figure 3 This is a cross-sectional view of the structure of a multi-channel microfluidic chip active valve.

[0030] Figure 4 This is a cross-sectional view of the structure of a multi-channel microfluidic chip active valve.

[0031] Figure 5 This is a cross-sectional view of the structure of a multi-channel microfluidic chip active valve.

[0032] Figure 6 for Figure 5 A magnified view of part A in the middle.

[0033] Figure 7 This is a cross-sectional view of the valve body structure.

[0034] Illustrations: 100, Chip body; 110, Valve body groove; 120, Sample inlet groove; 130, Sample channel; 140, Outlet; 150, Inlet; 160, Inlet channel; 170, Sample analysis cell; 200, Valve body structure; 1, Support layer; 11, Embedded groove; 2, Flow control layer; 21, Flow control component; 211, Through section; 212, Blocking section; 3, Clamping plate; 31, Elastomer; 32, Ear plate; 4, Drive section; 41, Cam; 42, Fixing plate; 43, Spring; 44, Elliptical wheel; 45, Fixing block; 46, Transverse groove; 47, Longitudinal groove. Detailed Implementation

[0035] To make the objectives, features, and advantages of this invention more apparent and understandable, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described below are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0036] In the description of this invention, it should be understood that the terms "upper," "lower," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. It should be noted that when a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be a component positioned centrally in the connection.

[0037] This invention provides a multi-channel microfluidic chip active valve, comprising a carrier layer and a flow control layer. The carrier layer extends along a first direction; the flow control layer is disposed on the carrier layer, and multiple sets of flow control components are arranged at intervals along the first direction on the flow control layer. Each set of flow control components includes a through portion for fluid to pass through and a blocking portion for blocking fluid. The surface of the flow control layer where the flow control components are disposed is a first side surface. The microfluidic chip has a second sealing surface that is in contact with the first sealing surface. Fluid inlet and outlet are disposed on the second sealing surface, and the first sealing surface is in contact with the second sealing surface. The flow control layer is a flexible material layer, and the carrier layer and the flow control layer form an integral valve body structure. The valve body structure reciprocates along the first direction to switch the alignment positions of the through portion and the blocking portion at the fluid inlet and outlet on the second sealing surface.

[0038] This invention provides a multi-channel microfluidic chip active valve. By integrating multiple spaced valve groups into the valve body structure, and controlling all flow control components to switch synchronously through unidirectional reciprocating movement, it achieves parallel control of simultaneously opening or closing multiple fluid channels. This effectively overcomes the shortcomings of single-channel switching and greatly improves the efficiency of high-throughput detection. Furthermore, the multi-channel microfluidic chip active valve consists only of a carrier layer and a flow control layer, eliminating the need for multi-layered structures and complex pneumatic control systems required by pneumatic diaphragm valves, and the need for assembling multiple independent valve bodies as with solenoid valves. The simplified integrated structure significantly reduces the manufacturing and assembly costs of the valve body structure, effectively improving the portability and integration of the microfluidic chip active valve. In addition, the soft rubber material used in the flow control layer, through its elastic deformation capability, allows the flow control layer to fit tightly against the chip in both blocked and open states, effectively sealing the flow channel interface, ensuring valve sealing, preventing fluid leakage and cross-contamination, and improving the accuracy and reliability of detection results. Therefore, the multi-channel microfluidic chip active valve provided in this embodiment of the invention can achieve parallel control of multi-channel fluids while ensuring high integration and miniaturization.

[0039] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0040] like Figures 1-7 As shown, this invention provides a multi-channel microfluidic chip active valve for use in microfluidic chips with multi-channel fluid control, such as in scenarios involving multi-index joint detection and multi-sample parallel analysis. Through structural improvements, this invention effectively resolves the contradictions in existing microfluidic valve technologies regarding parallel control, cost, integration, and portability, providing a microfluidic chip active valve that balances high integration and miniaturization while enabling multi-channel fluid parallel control.

[0041] like Figure 1 and Figure 2 As shown, in this embodiment, the multi-channel microfluidic chip active valve includes a carrier layer 1 and a flow control layer 2. The carrier layer 1 extends along a first direction; the flow control layer 2 is disposed on the carrier layer 1, and multiple sets of flow control components 21 are arranged at intervals along the first direction on the flow control layer 2. Each set of flow control components 21 includes a through portion 211 for fluid to pass through and a blocking portion 212 for blocking fluid. The surface of the flow control layer 2 where the flow control components 21 are disposed is a first sealing surface. The microfluidic chip has a second sealing surface that is in contact with the first sealing surface. Fluid inlet and outlet are disposed on the second sealing surface. The first sealing surface is in contact with the second sealing surface. The flow control layer 2 is a flexible material layer. The carrier layer 1 and the flow control layer 2 form an integral valve body structure 200. The valve body structure 200 is moved back and forth along the first direction to switch the through portion 211 and the blocking portion 212 at the alignment position with the fluid inlet and outlet on the second sealing surface.

[0042] Specifically, the carrier layer 1 can be an injection-molded, elongated plate-like structure extending along the first direction, providing a support for the parallel control of the multi-channel microfluidic chip. The fluid control layer 2 is an elongated structure made of a flexible rubber material with elastic deformation capabilities. The fluid control layer 2 can be tightly connected to the carrier layer 1 by means of casting and curing or bonding, thereby forming an integral, rigid-flexible valve body structure 200 with the carrier layer 1.

[0043] Meanwhile, multiple sets of flow control components 21 are arranged at intervals along the first direction on the flow control layer 2. Each set of flow control components 21 includes a through section 211 and a blocking section 212. The through section 211 allows fluid to pass through, while the blocking section 212 blocks the fluid. The distance between the through section 211 and the blocking section 212 in each set of flow control components 21 is consistent. This facilitates the simultaneous control of multiple parallel fluid channels.

[0044] For example, the microfluidic chip may have an adapter structure for assembling the valve body structure 200, such as a mounting groove or mounting cavity. The microfluidic chip has multiple parallel fluid channels, each with a fluid inlet and a fluid outlet connected to the valve body structure 200, and the fluid inlet and fluid outlet are in the same plane, which is the second sealing surface. The side of the flow control layer 2 with the through portion 211 and the blocking portion 212 is the first sealing surface. When the valve body structure 200 is assembled in the microfluidic chip, the first sealing surface and the second sealing surface are in contact, and each set of flow control components 21 corresponds to a set of fluid inlets and fluid outlets.

[0045] By driving the valve body structure 200 to reciprocate along a first direction, the distance it moves is equal to the distance between the through section 211 and the blocking section 212. When the through section 211 moves to align with the fluid inlet and fluid outlet, the fluid channel is opened, allowing fluid to pass through; when the blocking section 212 moves to align with the fluid inlet and fluid outlet, the fluid channel is blocked, thereby realizing the parallel control function of simultaneously opening or closing multiple fluid channels. This effectively overcomes the defects of single-channel switching and greatly improves the efficiency of high-throughput detection. Moreover, the multi-channel microfluidic chip active valve consists only of a carrier layer 1 and a flow control layer 2, eliminating the need for the multi-layer structure and complex pneumatic control system required by pneumatic diaphragm valves, and eliminating the need to assemble multiple independent valve bodies as with solenoid valves. The simplified integrated structure significantly reduces the manufacturing and assembly costs of the valve body structure 200, effectively improving the portability and integration of the microfluidic chip active valve. Therefore, it is possible to achieve parallel control of multi-channel fluids while ensuring high integration and miniaturization.

[0046] Furthermore, the carrier layer 1 has a groove 11 extending along a first direction, and the flow control layer 2 is embedded in the groove 11. The flow control layer 2, made of soft rubber, is embedded in the groove 11 and tightly connected to the carrier layer 1. The flow control component 21 is disposed on the upper surface of the flow control layer 2, which serves as the first sealing surface. The carrier layer 1 can be tightly assembled into the microfluidic chip via an interference fit, and the first sealing surface and the second sealing surface achieve a tight fit, ensuring both fluid channel control and a sealing effect between the valve body structure 200 and the microfluidic chip.

[0047] Furthermore, the through portion 211 is formed by a strip-shaped groove on the flow control layer 2 along the second direction, and the blocking portion 212 is formed by grooves spaced apart on the flow control layer 2 along the second direction. For example, when the through portion 211 is aligned with the fluid inlet and fluid outlet of the fluid channel, the fluid can maintain the continuity of the fluid channel through the strip-shaped groove; when the blocking portion 212 is aligned with the fluid inlet and fluid outlet of the fluid channel, the fluid inlet and fluid outlet are aligned with the two grooves respectively, thereby blocking the fluid channel.

[0048] For example, the portion of the first sealing surface with the flow control component 21 protrudes outward. That is, the portion of the upper surface of the flow control layer 2 with the through portion 211 and the blocking portion 212 protrudes in the direction away from the bearing layer 1, so that the flow control layer 2 at the positions of the through portion 211 and the blocking portion 212 fits more tightly with the second sealing surface, further improving the sealing effect of the valve body structure 200 and effectively preventing fluid leakage.

[0049] like Figure 5 , Figure 6 As shown, in this embodiment of the invention, the multi-channel microfluidic chip active valve further includes a drive unit 4 for driving the valve body structure 200 to reciprocate along a first direction. The moving distance of the valve body structure 200 is consistent with the distance between the through portion 211 and the blocking portion 212 in the same group of flow control components 21. Since the distance between the through portion 211 and the blocking portion 212 in each group of flow control components 21 is consistent, and each group of flow control components 21 corresponds to a set of fluid inlets and fluid outlets, the distance between multiple groups of flow control components 21 can be specifically set according to the distance between multiple parallel fluid channels. Thus, when the drive unit 4 drives the valve body structure 200 to reciprocate along the first direction, the through portion 211 and the blocking portion 212 can be aligned with the fluid inlet and fluid outlet on the second sealing surface, respectively, to achieve parallel control of simultaneously opening or closing multiple fluid channels.

[0050] The drive unit 4 can be an electric actuator, with its telescopic end connected to the flow control layer, thereby driving the flow control layer 2 to reciprocate; or, a sliding hole extending in a first direction can be formed on the support layer 1, with a control rod slidably disposed within the sliding hole, the control rod connected to the flow control layer 2, thereby driving the flow control layer 2 to reciprocate by manipulating the control rod. The present invention does not impose any special limitations on the driving method of the drive unit 4.

[0051] In an exemplary embodiment of the present invention, two symmetrically arranged clamping plates 3 are slidably disposed within the groove 11, and a flow control layer 2 is disposed between the two clamping plates 3. An elastic body 31 is disposed between the clamping plates 3 and the sidewall of the groove 11. Specifically, the two clamping plates 3 are arranged along a first direction and are located on opposite sides within the groove 11. The flow control layer 2 is disposed between the two clamping plates 3, and the elastic body 31 is disposed between the clamping plates 3 and the sidewall of the groove 11. The elastic body 31 is tightly connected to the clamping plates 3 and the groove 11 by adhesive bonding, and the elastic deformation force of the elastic body 31 is greater than the elastic deformation force of the flow control layer 2. For example, driving the two clamping plates 3 to move away from each other compresses the elastic body 31, thereby reducing the degree of adhesion between the flow control layer 2 and the support layer 1, facilitating the movement of the flow control layer 2. After the flow control layer 2 moves an appropriate distance, the elasticity of the elastic body 31 can drive the two clamping plates to move closer to each other, so that the flow control layer 2 and the support layer 1 are tightly adhered again.

[0052] For example, when the valve body structure 200 needs to move in the first direction, the two clamping plates 3 can be moved away from each other, reducing the adhesion between the flow control layer 2 and the chip body 100 to facilitate the movement of the valve body structure 200. After the movement is completed, the two clamping plates 3 return to their original positions, clamping the flow control layer 2 again to improve the sealing effect between the flow control layer 2 and the microfluidic chip. It should be noted that the movement of the two clamping plates 3 only changes the adhesion force between the flow control layer 2 and the chip body 100, and does not completely disengage the flow control layer 2 from the chip body 100. Therefore, the flow control layer 2 can always maintain adhesion to the chip body 100 to ensure the sealing effect during the movement of the valve body structure 200.

[0053] Furthermore, when the drive unit 4 moves the valve body structure 200, it can simultaneously drive the two clamping plates 3 to move away from each other. This causes the degree of contact between the flow control layer 2 and the chip body 100 to decrease during the switching process and recover after the switching is completed, ensuring the sealing effect between the flow control layer 2 and the chip body 100 while facilitating the switching of the flow control layer 2.

[0054] like Figure 5 , Figure 6 As shown, in an exemplary embodiment, the driving unit 4 can drive the valve body structure 200 to move along the first direction while simultaneously driving the two clamping plates 3 to move away from each other by rotating the cam 41. For example, a transverse sliding groove 46 is formed on the mounting groove of the microfluidic chip assembling valve body structure 200, and a fixing plate 42 is fixedly connected to the bearing layer 1. The fixing plate 42 is located in the transverse sliding groove 46, and a spring 43 along the first direction is provided between the fixing plate 42 and the side wall of the transverse sliding groove 46. The cam 41 is rotatably connected to the microfluidic chip, and the cam 41 abuts against the fixing plate 42. When the cam 41 is rotated, the spring 43 keeps the fixing plate 42 in contact with the cam 41, thereby allowing the valve body structure 200 to reciprocate. Meanwhile, the bottom of the groove 11 of the bearing layer 1 is symmetrically provided with longitudinal sliding grooves 47, and the bottoms of the two longitudinal sliding grooves 47 are connected to form a sliding cavity; the lower end of the clamping plate 3 is fixedly connected with an ear plate 32, which is located in the longitudinal sliding groove 47. An elliptical wheel 44 is provided in the sliding cavity, and the two sides of the elliptical wheel 44 with smaller diameter abut against the two ear plates 32 respectively. The elliptical wheel 44 is coaxially arranged with the cam 41. Therefore, when the cam 41 is rotated, the cam 41 drives the valve body structure 200 to reciprocate along the first direction. At the same time, the elliptical wheel 44 drives the two clamping plates 3 to move away from each other first, and then move closer to each other under the reset of the elastic body 31. The rotation of the cam 41 can be automatically controlled by a motor or electric push rod, or it can be manually controlled.

[0055] like Figure 7As shown, in another exemplary embodiment, the drive unit 4 uses a motor, electric push rod, or manual operation to drive the valve body structure 200 to move along the first direction, and the two clamping plates 3 can move away from each other during the movement. For example, longitudinal grooves 47 are symmetrically opened at the bottom of the groove 11 of the bearing layer 1, and the bottoms of the two longitudinal grooves 47 are connected to form a sliding cavity; an ear plate 32 is fixedly connected to the lower end of the clamping plate 3, the ear plate 32 is located in the longitudinal groove 47 and its end face is a pointed surface with two symmetrical inclined surfaces, a rhomboid fixing block 45 is provided in the sliding cavity, the fixing block 45 is fixedly connected to the chip body 100; and the two adjacent inclined surfaces of the fixing block 45 along the second direction are respectively in contact with the inclined surfaces on the same side of the two ear plates 32. When the drive unit 4 moves the valve body structure 200, the ear plate 32 is driven by the inclined surface that is in contact with the fixing block 45, which can drive the two clamping plates 3 to move away from each other first, so that the deformation of the flow control layer 2 is reduced and the adhesion between the flow control layer 2 and the chip body 100 is reduced; then, the ear plate 32 contacts the inclined surface on the other side of the fixing block 45, so that under the elastic force of the elastic body 31, the two clamping plates 3 can move closer to each other again, so that the deformation of the flow control layer 2 is increased and the adhesion between the flow control layer 2 and the chip body 100 is increased.

[0056] In one embodiment of the present invention, the carrier layer 1 is made of a rigid adhesive material, such as polycarbonate (PC), polystyrene (PS), polymethyl methacrylate (PMMA), cyclic olefin copolymer (COC), or cyclic olefin polymer (COP). The flow control layer 2 is made of a soft adhesive material, such as silicone or thermoplastic elastomer. The soft adhesive material used in the flow control layer 2 utilizes its own elastic deformation capability to ensure a tight fit between the flow control layer 2 and the chip in both blocked and open states, effectively sealing the flow channel interface, ensuring the valve's sealing performance, preventing fluid leakage and cross-contamination, and improving the accuracy and reliability of the detection results.

[0057] like Figure 3 , Figure 4As shown, this embodiment of the invention also provides a microfluidic chip, including a chip body 100 and the aforementioned multi-channel microfluidic chip active valve. The chip body 100 has a valve body groove 110 for assembling the valve body structure 200, and the second sealing surface is the groove wall of the valve body groove 110. A sample inlet groove 120 is formed on the upper surface of the chip body 100, and its interior has a sample channel 130 communicating with the sample inlet groove 120. The sample channel 130 branches into multiple sub-channels, and the end of each sub-channel is connected to the second sealing surface and forms an outlet 140. The chip body 100 also has multiple channels arranged at intervals along a first direction. The sample analysis tank 170 has multiple sample analysis tanks 170 corresponding to multiple sub-channels. Each sample analysis tank 170 is connected to a liquid inlet channel 160. The inlet end of the liquid inlet channel 160 is connected to the second sealing surface and forms a liquid inlet 150. The liquid inlet 150 and the liquid outlet 140 are arranged at intervals along the second direction. A transparent observation window can be provided at the opening of the sample analysis tank 170. Each set of flow control components 21 corresponds to a set of liquid inlets 150 and liquid outlets 140. The through part 211 or the blocking part 212 is aligned with the liquid inlet 150 and the liquid outlet 140, respectively, to realize the on / off control between the sub-channel and the liquid inlet channel 160.

[0058] Specifically, the valve body groove 110 is adapted to the valve body structure 200. Positioning blocks can be installed on the groove wall of the valve body groove 110. When the valve body structure 200 slides in the valve body groove 110, and the through-hole 211 and the blocking part 212 align with the liquid outlet 140 and liquid inlet 150 respectively, a matching positioning groove can be installed on the bearing layer 1 at the position corresponding to the positioning block, facilitating precise alignment of the flow control component 21 with the liquid outlet 140 and liquid inlet 150. Sample liquid enters the sample channel 130 from the sample inlet groove 120 and is distributed to each sub-channel. When the through-hole 211 aligns with the liquid inlet 150 and liquid outlet 140, the liquid can enter the inlet channel 160; when the blocking part 212 aligns with the liquid inlet 150 and liquid outlet 140, the liquid is blocked. This allows for parallel control of simultaneously opening or closing multiple fluid channels.

[0059] The above-described embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-channel microfluidic chip active valve, characterized in that, The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve.

2. The multi-channel microfluidic chip active valve of claim 1, wherein, The application relates to a multichannel microfluidic chip active valve.

3. The multi-channel microfluidic chip active valve of claim 2, wherein, The application relates to a multichannel microfluidic chip active valve.

4. The multi-channel microfluidic chip active valve of claim 3, wherein, The application relates to a multichannel microfluidic chip active valve.

5. The multi-channel microfluidic chip active valve of claim 2, wherein, The application relates to a multichannel microfluidic chip active valve.

6. The multi-channel microfluidic chip active valve of claim 1, wherein, The application relates to a multichannel microfluidic chip active valve.

7. The multi-channel microfluidic chip active valve of claim 1, wherein, The application relates to a multichannel microfluidic chip active valve.

8. A microfluidic chip, characterized by, The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel microfluidic chip active valve. The application relates to a multichannel A plurality of sample analysis grooves (170) are arranged at intervals along a first direction inside the chip body (100), and the plurality of sample analysis grooves (170) correspond to the plurality of sub-channels one by one. Each sample analysis groove (170) is communicated with a liquid inlet channel (160), the inlet end of the liquid inlet channel (160) is communicated to the second sealing surface and forms a liquid inlet (150), and the liquid inlet (150) and the liquid outlet (140) are arranged at intervals along a second direction. Each group of flow control components (21) corresponds to a group of liquid inlets (150) and liquid outlets (140), and the through portions (211) or the blocking portions (212) are aligned with the liquid inlets (150) and the liquid outlets (140) respectively, so as to realize the on-off control between the sub-channels and the liquid inlet channels (160).

Images