Ganoderma lucidum sporocarp large honeyed bolus and preparation method thereof

Abstract

The invention belongs to the field of food health care, and particularly relates to a ganoderma lucidum sporocarp big honeyed pill and a preparation method thereof. The method disclosed by the invention comprises the following steps: 1) treating ganoderma lucidum sporocarp, namely slicing, firstly carrying out oxidase inactivation and drying, then carrying out liquid nitrogen ultralow-temperature treatment, and pulverizing the obtained ganoderma lucidum sporocarp ice crystals by adopting an ultrafine pulverizer to obtain ganoderma lucidum sporocarp ultrafine powder; the honey is correspondingly refined; and mixing the ganoderma lucidum sporocarp superfine powder with refined honey to prepare a formed honeyed pill, and then drying and packaging the honeyed pill. The honey serving as a natural excipient is uniformly mixed with the crushed ganoderma lucidum sporocarp superfine powder, so that the mouth feel is improved, the preservation time is prolonged, and long-term preservation and taking are facilitated.

Description

Technical Field

[0001] This invention belongs to the field of food and health care, specifically relating to a large honey pill made from Ganoderma lucidum fruiting body and its preparation method. Background Technology

[0002] Reishi mushroom is known as the "immortal herb" and has been included in the list of raw materials for health food, playing an important role in the field of health.

[0003] Chinese patent CN201210098285.8 discloses a Ganoderma lucidum pill and its processing method. The method uses ethanol-extracted Ganoderma lucidum extract as the main ingredient and broken-cell wall Ganoderma lucidum spore powder as an auxiliary ingredient. The ethanol-extracted Ganoderma lucidum extract obtained through ethanol column gradient percolation and the broken-cell wall Ganoderma lucidum spore powder are mixed evenly in a mixer, formed into pills, dried, coated, cooled, and shaped to form Ganoderma lucidum pills. The unique triterpenoids and crude polysaccharides in these Ganoderma lucidum pills have significant effects on the human body. They can inhibit tumor growth, reduce adverse reactions caused by chemotherapy and radiotherapy, improve the efficiency of tumor treatment, improve the quality of life and prolong the survival of cancer patients, enhance the body's immune function, regulate antibody levels, and suppress autoimmunity. They also have liver-protecting and detoxifying effects, lower blood pressure, treat diabetes, improve cardiovascular health, and improve sleep.

[0004] Chinese patent CN202010735379.6 discloses a method for preparing Ganoderma lucidum pills, in which Ganoderma lucidum is sliced ​​into thin slices and distilled to obtain a solution with an alcohol content of 50-95%. 。 The edible wine is used as the extraction solvent and mixed evenly with Ganoderma lucidum slices. The moistened Ganoderma lucidum slices are layered in a percolation device and percolated multiple times with edible wines of different alcohol contents to obtain an extract. The obtained extract is vacuum concentrated, spray dried and ground to make Ganoderma lucidum extract powder. Using Ganoderma lucidum extract powder as the main ingredient, Ganoderma lucidum ultrafine powder as the auxiliary ingredient and refined honey as the binder, the extract is made into pills and coated with a coating to obtain Ganoderma lucidum pills.

[0005] From the perspective of raw materials, pills made from Ganoderma lucidum extract and broken-cell wall spore powder, while possessing high medicinal and health-promoting value, still have potential drawbacks: the preparation of the extract requires high-temperature extraction and concentration, which may lead to the degradation of heat-sensitive active ingredients (such as polysaccharides and some triterpenes); the bitterness of Ganoderma lucidum extract intensifies after concentration, while spore powder is slightly bitter and has a slightly earthy taste, which may reduce consumer acceptance, requiring the addition of flavoring agents; the high sugar content of the extract makes it prone to absorbing moisture and clumping, affecting the hardness and long-term storage stability of the pills; traditional honey pills rely on honey for binding, but the viscosity of the extract itself may interfere with the honey. The binding effect requires adjusting the honey ratio or adding other binders (such as starch); after the cell wall of the spore powder is broken, the unsaturated fatty acids are easily oxidized and rancid, shortening the shelf life; the extract has high viscosity, while the cell wall broken spore powder is light and fluffy, and the two have large differences in physical properties, making them prone to stratification when mixed, resulting in uneven distribution of active ingredients in the pills; the extract is highly hygroscopic, while the cell wall broken spore powder contains oily components, so the temperature and humidity must be strictly controlled when drying the pills, otherwise surface cracking or internal moisture residue may occur; the price of the cell wall broken spore powder is much higher than that of ordinary Ganoderma lucidum fruiting bodies, and the preparation of the extract requires a large amount of raw materials, increasing production costs.

[0006] From a technological perspective, during the percolation process, to ensure good extraction results, the Ganoderma lucidum needs to be compressed to a certain density. The traditional method involves adding the Ganoderma lucidum into the percolation cylinder while simultaneously compacting it with a stick. This compaction method often results in varying densities at different locations within the same height or at different heights within the same location. Due to these varying densities, ethanol solution easily penetrates the less dense Ganoderma lucidum, while less dense Ganoderma lucidum receives ethanol solution penetration. This leads to the less dense Ganoderma lucidum being fully soaked, resulting in less extracted components and underutilization of the Ganoderma lucidum raw material, thus increasing production costs.

[0007] Currently, when using Ganoderma lucidum fruiting bodies as raw materials to prepare corresponding products, the methods typically involve traditional physical processing and chemical extraction. Traditional physical processing methods include washing the fruiting bodies → slicing and crushing → continuous drying at 60-80℃ for 10-15 hours → producing processed tea slices or coarse powder. The drying process, using a single temperature, leads to surface hardening, internal moisture residue, high energy consumption, and low efficiency. Chemical extraction methods include water / alcohol extraction: the fruiting bodies are crushed and then extracted with water or ethanol → concentrated → dried to obtain an extract powder; acid / alkali-assisted methods: the fruiting bodies are treated with hydrochloric acid or alkali to improve the polysaccharide extraction rate; and supercritical CO2 extraction: high-pressure CO2 extraction of triterpenoids. Chemical extraction methods often result in the destruction of heat-sensitive components, and high temperatures or strong acids and alkalis cause degradation of polysaccharides and triterpenoids. There is also a risk of solvent residue, and the process involves multiple filtrations and concentrations, resulting in low industrial efficiency and cumbersome steps. Summary of the Invention

[0008] The technical problem to be solved by the present invention is to provide a large honey pill of Ganoderma lucidum fruiting body and its preparation method.

[0009] To solve the above-mentioned technical problems, the present invention provides a method for preparing large honey pills from Ganoderma lucidum fruiting bodies, comprising the following steps: 1) Processing of Ganoderma lucidum fruiting bodies a. Slicing: Select fresh Ganoderma lucidum fruiting bodies, clean them thoroughly (to remove surface dust and impurities), and slice them (approximately 3-5 mm thick). b. Divided into the following two stages: The first stage of oxidase inactivation and drying: oxidase is inactivated by microwave inactivation, which makes it less likely for the Ganoderma lucidum fruiting body to turn black. Then, under natural ventilation conditions (about 0.8~1.0 m / s), it is treated at 75±5℃ for 2±0.5 h, which reduces the water content of the fruiting body. The second stage involves ultra-low temperature treatment with liquid nitrogen at -196℃, which instantly forms ice crystals on the fruiting body of Ganoderma lucidum. c. Pulverization: The ice crystals of Ganoderma lucidum fruiting body are immediately pulverized using an ultra-micro pulverizer to obtain ultra-micro powder of Ganoderma lucidum fruiting body (D90<45μm). 2) Selection and refining of auxiliary materials Honey is used as an auxiliary ingredient; First, boil the bees at 90±1℃ and keep it at that temperature for 5±0.5 min (to remove impurities, destroy enzymes, kill bacteria, evaporate moisture, and increase viscosity). Simmer at 85±1℃ for 15±0.5 min (at this point, the honey will turn light yellow, form threads, and sink to the bottom without dispersing in water); this yields the refined honey. 3) Mixed raw materials Mix the ultrafine powder of Ganoderma lucidum fruiting body obtained in step 1) with the refined honey obtained in step 2) at a mass ratio of 1:(1.5±0.1) and stir evenly (to ensure that the components are fully integrated). 4) Molding The mixture obtained in step 3) is prepared into pellets to obtain shaped honey pills; 5) Drying and Packaging The shaped honey pills obtained in step 4) are dried at low temperature until the moisture content is ≤15% (to remove excess moisture and maintain the stability of its nutritional components). Then the dried honey pills are packaged and sealed for storage.

[0010] As an improvement to the preparation method of the large honey pills of Ganoderma lucidum fruiting bodies of the present invention: the microwave inactivation in step 2) is as follows: the microwave generator power is 3~5kW, the frequency is 2450MHz, the inactivation time is 6~8 min, and the microwave temperature is 50~70℃.

[0011] As a further improvement to the preparation method of the large honey pills of Ganoderma lucidum fruiting bodies of the present invention: In step 5), the low-temperature drying temperature is 40±5 ℃.

[0012] As a further improvement to the preparation method of the large honey pills of Ganoderma lucidum fruiting bodies of the present invention: In step 4), the particle size of the formed honey pills is 9±1 mm.

[0013] The present invention also provides large honey pills of Ganoderma lucidum fruiting bodies prepared by any of the methods described above.

[0014] This invention has good market prospects and meets the modern demand for healthy food.

[0015] In step 4) of this invention: pellets of appropriate thickness (approximately 9±1 mm in diameter) are prepared for easy pelletizing. The pellets should be uniform in thickness, smooth in surface, and solid without voids. A pellet rolling board is used for small-scale preparation, while a pellet rolling machine is used for mass production. The pellets are placed in a mold and pressed to form honey pellets. A pellet rolling board can be used for manual pellet making, while a pellet rolling machine is used for mass production.

[0016] In the processing of Ganoderma lucidum fruiting bodies in this invention, a first-stage oxidase inactivation and drying at 75±5℃ are performed, followed by a second-stage ultra-low temperature treatment with liquid nitrogen, thereby instantly forming ice crystals in the Ganoderma lucidum fruiting bodies. This process balances pulverization efficiency with the preservation of effective components such as Ganoderma lucidum polysaccharides and triterpenes, and is far superior to traditional continuous high-temperature pulverization methods.

[0017] This invention uses honey as a formulator instead of chemical binders to enhance the dissolution of Ganoderma lucidum components while masking the bitterness of Ganoderma lucidum triterpenoids. No additional flavoring agents are needed. In addition, honey has natural antibacterial properties, which can extend the product's shelf life.

[0018] Currently, deep processing of Ganoderma lucidum fruiting bodies is still dominated by extracts (accounting for 70% of the market). This product innovates the use of raw materials, skipping the extraction process and directly using the whole fruiting body powder as medicine, avoiding the loss of components in the preparation of extracts. The entire process is free of organic solvents / strong acids and alkalis, which is in line with the trend of green production of health food. Drying and mechanical pelleting (pill strip machine / rolling machine) can achieve continuous production. Through the innovative combination of physical cell wall breaking and honey exfoliation, the integrity of natural products is preserved while enabling direct consumption, providing a new path for the high-value development of Ganoderma lucidum.

[0019] The Ganoderma lucidum honey pills prepared by this invention are rich in polysaccharides, triterpenes, and other components, which can significantly improve immunity and regulate intestinal flora, making them suitable for the development of health food products. This invention relates to a method for preparing Ganoderma lucidum honey pills that preserves as many of the effective components of Ganoderma lucidum as possible and fully utilizes the value of the Ganoderma lucidum fruiting body.

[0020] In summary, this invention uses honey as a natural excipient and mixes it evenly with pulverized Ganoderma lucidum fruiting body powder (Ganoderma lucidum fruiting body ultrafine powder) to improve the taste, extend the shelf life, and facilitate long-term storage and consumption.

[0021] The Ganoderma lucidum fruiting body honey pill product of the present invention can be mixed with other excipients that replenish qi, nourish blood, and calm the mind (medicinal and edible substances that conform to the "List of Raw Materials Available for Health Foods" such as mulberry, black sesame, wolfberry, red dates, etc.), and achieve health care effects from multiple levels through the synergistic effect of multiple active ingredients.

[0022] Directions for use: Ready to eat after opening the bag; chew directly.

[0023] Dosage: According to the specific product instructions, the general adult daily dose is about 1 to 2 pills. The specific dosage should be based on the instructions or the doctor's advice.

[0024] When to take: Can be taken at any time.

[0025] Friendly reminder: Children under seven years old should consume this product under adult supervision. Attached Figure Description

[0026] The specific embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0027] Figure 1 A curve of glucose standard solution; Figure 2 Curve for ursolic acid standard solution; Figure 3 This is a picture of the finished product of Lingzhi honey pills. Detailed Implementation

[0028] The present invention will be further described below with reference to specific embodiments, but the scope of protection of the present invention is not limited thereto: Example 1: A method for preparing Ganoderma lucidum honey pills, comprising the following steps: 1) Processing of Ganoderma lucidum fruiting bodies a. Slicing: Select fresh Ganoderma lucidum fruiting bodies, clean them thoroughly (to remove surface dust and impurities, etc.), and slice them (approximately 3-5 mm thick). b. It consists of the following two stages: The first stage involves oxidase inactivation and drying: Oxidase is inactivated using microwave inactivation to prevent the Ganoderma lucidum fruiting bodies from turning black. The microwave generator has a power of 5kW, a frequency of 2450MHz, an inactivation time of 8 minutes, and a microwave temperature of 60±5℃. After inactivation, the fruiting bodies are treated at 75±5℃ for 2 hours under natural ventilation conditions (approximately 0.8~1.0 m / s) to reduce the moisture content (to less than 35%). The second stage involves ultra-low temperature treatment with liquid nitrogen at -196℃, which instantly forms ice crystals on the fruiting bodies of Ganoderma lucidum.

[0029] c. Pulverization: The ice crystals of Ganoderma lucidum fruiting bodies are immediately pulverized using an ultra-micro pulverizer (preferably an ultra-low temperature ultra-micro pulverizer) to obtain ultra-micro powder of Ganoderma lucidum fruiting bodies (D90<45μm).

[0030] 2) Selection and refining of auxiliary materials Honey is used as an auxiliary ingredient; First, boil the bees at 90±1℃ and keep it for 5 minutes (to remove impurities, destroy enzymes, kill bacteria, evaporate moisture, and enhance viscosity). Simmer at 85±1℃ for 15 minutes (at this point, the honey will turn light yellow, form threads, and sink to the bottom without dispersing in water); this yields the refined honey.

[0031] 3) Mixed raw materials Mix the ultrafine powder of Ganoderma lucidum fruiting body obtained in step 1) with the refined honey obtained in step 2) at a mass ratio of 1:1.5 and stir evenly (to ensure that the components are fully integrated).

[0032] 4) Molding The mixture obtained in step 3) is formed into pellets of appropriate thickness (approximately 9 mm in diameter) for easy pelletizing. The pellets should be uniform in thickness, smooth in surface, and solid without voids. A pellet rolling board is used for small-scale preparation, while a pellet rolling machine is used for mass production. The pellets are placed in a mold and pressed to form honey pellets. Pelletizing can be done manually using a pellet rolling board, while a pellet rolling machine is used for mass production. The resulting shaped honey pellets have a particle size of approximately 9 mm.

[0033] 5) Drying and Packaging The shaped honey pills obtained in step 4) are dried at a low temperature of 40°C until the moisture content is ≤15% (to remove excess moisture and maintain the stability of its nutritional components). Then, the dried honey pills are packaged in a conventional manner and sealed for storage.

[0034] Experiment 1: High-performance liquid chromatography-mass spectrometry analysis of active ingredients in water extracts of Ganoderma lucidum fruiting bodies To detect whether the active ingredients of Ganoderma lucidum fruiting bodies were lost after the two-stage process of this invention, high performance liquid chromatography-mass spectrometry analysis of the active ingredients was performed on its water extract.

[0035] 1. Sample preparation: Weigh an appropriate amount of the ultrafine powder of Ganoderma lucidum fruiting body obtained in step 1) of Example 1, add deionized water at a material-to-liquid ratio of 1:30 (g / mL), extract in a 90 ℃ water bath for 2 h, extract twice, combine the two aqueous extracts, concentrate under reduced pressure at 65 ℃ to 30% of the original volume, and then dry (dry at 65 ℃ for 48 h) to obtain the aqueous extract of Ganoderma lucidum fruiting body.

[0036] The concentration of the water extract of Ganoderma lucidum fruiting body was diluted to 10 mg / mL with ultrapure water, filtered through a 0.22 μm filter, and then placed into a liquid chromatography bottle for use in liquid chromatography. The ultrapure water filtered through the 0.22 μm filter was used as a blank solution.

[0037] 2. Chromatographic conditions: ACQUITY UPLCTMI-Class and Xevo G3XS liquid chromatography-mass spectrometry (LC-MS) were used for separation. A Waters BEH T3 1.8 m (2.1 mm × 150 mm) column was employed. The mobile phase consisted of 0.1% formic acid (A) and acetonitrile (B) aqueous solution. The flow rate was 0.3 mL / min, the injection volume was 3 µL, and the column temperature was 40 ℃. Elution conditions are shown in Table 1.

[0038] Table 1 Gradient elution conditions for high performance liquid chromatography

[0039] Positive ion mode was used for testing. The sampled mass range was 50–1200 Da. The parameters were set as follows: capillary voltage 3 kV; ion source temperature 100 °C; nebulizer temperature 280 °C; nebulizer flow rate 800 L / h; cone voltage 40 V; collision energy was low / high (6 V / 20–30 V).

[0040] Using the established chromatographic and mass spectrometric conditions, and comparing with the Waters Traditional Medicine database, the chemical constituents of Ganoderma lucidum fruiting body extract were analyzed using UNIFI 1.9.2 software. Considering both retention time and mass spectrometric data, 18 triterpenoids, 7 esters, 3 nucleosides, 9 sterols, and 12 flavonoids were identified in the aqueous extract of Ganoderma lucidum fruiting body. The triterpenoids included ganoderic acid A, ganoderic acid B, ganoderic acid G, ganoderic acid H, ganoderic acid beta, ganoderic acid delta, ganoderic acid epsilon, ganoderic acid theta, ganoderic acid Z, ganoderic lactone, and ganode-8-en-ricacid LM2. LM2 and other compounds were detected; sterols included β-sitosterol and 22,23-dihydrostigmasterol; flavonoids included apigenin, pinocembrin, acacetin and their glycoside derivatives; esters included acetylated and malonylated glycosides (such as 6''-O-Malonyldaidzin). In addition, organic acids, alkaloids and polyphenols were also detected.

[0041] Experiment 2: Detection of active ingredient content in Ganoderma lucidum fruiting bodies:

[0042] (1) Detection of polysaccharide content in Ganoderma lucidum fruiting bodies: a. Preparation of polysaccharide standard solution Accurately measure anhydrous glucose standard and dissolve it in purified water to prepare a stock solution with a concentration of 0.12 mg / mL. Weigh 0.1 g of analytical grade anthrone and mix it with 100 mL of 80% sulfuric acid to prepare an anthrone sulfate solution as an anthrone colorimetric reagent, and store it protected from light.

[0043] A gradient concentration of 0.2 mL was designed. 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, 1.0 mL, and 1.2 mL of glucose standard solution were added to test tubes, respectively. Distilled water was added to a final volume of 2 mL. The volume was then quickly brought to 8 mL with the prepared anthrone colorimetric reagent and mixed thoroughly. This yielded polysaccharide standard sample test solutions with concentrations of 3 μg / mL, 6 μg / mL, 9 μg / mL, 12 μg / mL, 15 μg / mL, and 18 μg / mL. After mixing, the solutions were allowed to stand in the dark for 15 min to allow the colorimetric reaction to occur. The colorimetric solution was then transferred to an ice bath and cooled for 15 min to terminate the reaction. The absorbance was then measured at 625 nm using ultraviolet spectrophotometry.

[0044] Table 2 Detection of glucose standard solution

[0045] R 2 Correlation coefficient formula:

[0046] Where R 2 Represents the correlation coefficient of the regression equation. A standard curve was plotted with anhydrous glucose concentration (X, μg / mL) on the x-axis and absorbance (Y) on the y-axis (e.g., ...). Figure 1 The regression equation is Y = 0.044X - 0.024, R0 2 =0.9975 (n=6).

[0047] b. Determination of polysaccharide content in Ganoderma lucidum fruiting bodies Accurately weigh 2.00 g of the Ganoderma lucidum fruiting body ultrafine powder obtained in step 1) of Example 1, add 60 mL of deionized water and let it swell at room temperature for 1 h. Connect a reflux condenser and reflux in a water bath at 70±5 °C for 4 h. Filter while hot, add an equal amount of deionized water to the filter residue, and reflux the filter residue again for 3 h. Combine the filtrates obtained from the two processes.

[0048] The collected filtrate was concentrated to a paste (approximately 5g) in a 65°C water bath. It was reconstituted with 5 mL of purified water, and then slowly mixed with 75 mL of ethanol. The mixture was incubated at 4°C for 12 h. The prepared solution was centrifuged at 4000 rpm, 25°C, and 10 min. The supernatant was discarded, and the precipitate was dissolved in 5 mL of 65°C hot water and brought to a final volume of 50 mL with purified water. The mixture was centrifuged again under the same conditions. 3 mL of the supernatant was taken and brought to a final volume of 25 mL with purified water to obtain the Ganoderma lucidum polysaccharide test solution. The test solution was diluted a certain factor and allowed to stand in the dark for 15 min to allow for color development. The colorimetric solution was transferred to an ice bath and cooled for 15 min to terminate the colorimetric reaction. Then, using a UV spectrophotometer, 200 μL of the test solution was measured at a wavelength of 625 nm, and the absorbance was recorded.

[0049] Based on the obtained standard curve, the sample to be tested was diluted 70 times, and the absorbance was 0.698. Substituting this into the standard curve Y=0.044X-0.024, the concentration of the diluted sample was found to be 16.4 μg / mL. Therefore, the sugar content of the Ganoderma lucidum fruiting body ultrafine powder obtained in step 1) of Example 1 is approximately 1.43%, which meets the requirement in the Chinese Pharmacopoeia that Ganoderma lucidum contains no less than 0.9% Ganoderma lucidum polysaccharides.

[0050] (2) Detection of triterpenoid and sterol content a. Preparation of standard solutions Accurately weigh ursolic acid standard and dissolve it in methanol to prepare a 0.2 mg / mL standard solution. Accurately weigh 500 mg of vanillin, dissolve it in glacial acetic acid, and dilute to a 10 mL amber volumetric flask. Mix well to obtain a 50 mg / mL colorimetric working solution, and prepare immediately before use.

[0051] The indicator was prepared by mixing the colorimetric working solution and perchloric acid in a volume ratio of 1:4.

[0052] Five groups of ursolic acid standard solutions were transferred to test tubes at concentrations of 0.1 mL, 0.2 mL, 0.3 mL, 0.4 mL, and 0.5 mL, respectively, and evaporated to dryness. A total of 1 mL of indicator was added. The reaction system was transferred to a water bath and heated at 70°C for 15 min, followed by an ice bath to terminate the reaction for 5 min. Ethyl acetate was added to each of the different experimental levels of the reaction system to bring the volume to 5 mL, and after mixing, the absorbance was measured at 550 nm.

[0053] Table 3 Detection of ursolic acid standard solution

[0054] A standard curve was plotted with ursolic acid concentration (X, μg / mL) on the x-axis and absorbance (Y) on the y-axis (e.g., ...). Figure 2 The regression equation is obtained as Y = 0.0088X - 0.0068, R0 2 =0.9989 (n=5).

[0055] b. Determination of triterpenoid and sterol content in Ganoderma lucidum samples Take 2 g of the ultrafine powder of Ganoderma lucidum fruiting body obtained in step 1) of Example 1, add 50 mL of ethanol, extract by ultrasonication for 45 min (140 W, 42 kHz, 45 °C), filter, add ethanol to the filtrate to make up to 100 mL, and then dilute to a certain multiple (take 1 mL and add ethanol to make up to 10 mL) to prepare the Ganoderma lucidum sample triterpenoid and sterol test solution. Take 200 μL and measure the absorbance at a wavelength of 550 nm.

[0056] The obtained absorbance was 0.125; substituting it into the standard curve "Y=0.0088X-0.0068", the concentration of the test solution was found to be 14.98 μg / mL. Therefore, the Ganoderma lucidum sample contained approximately 0.75% triterpenes and sterols, meeting the requirement of the 2020 edition of the Chinese Pharmacopoeia that Ganoderma lucidum should contain no less than 0.5% triterpenes and sterols.

[0057] (3) Quality testing of Ganoderma lucidum honey pills According to the Chinese Pharmacopoeia (2020 Edition), 20 pills of the test sample were taken, and the total weight was accurately measured. After obtaining the average weight of the pills, the weight of each pill was then accurately measured. The weight of each pill was compared with the labeled weight. No more than 2 pills exceeded the weight difference limit, and no single pill exceeded the limit by more than 100%. For the Lingzhi honey pills, 20 pills were taken, and the total weight was accurately measured. After obtaining the average weight of the pills, the weight of each pill was then accurately measured. The weight of each pill was compared with the labeled weight. All pills met the weight difference limit of ±7.5%.

[0058] The Ganoderma lucidum honey pills are dark brown, smooth, uniformly spherical, and have a moisture content of less than 13%. Each Ganoderma lucidum honey pill weighs 0.6 g and has a particle size of 9 mm.

[0059] Take 6 test tablets, select a sieve basket with an aperture of about 1.0 mm, and test them with a baffle according to the method of disintegration time test (General Chapter 0921) under the tablets. If they completely disintegrate within 1 hour, it meets the disintegration time limit requirements of the Chinese Pharmacopoeia (2020 edition).

[0060] Comparative Example 1: Step b of step 1) in Example 1 is modified as follows: The oxidase was inactivated and dried using only microwave inactivation, with the microwave inactivation process conditions being the same as in Example 1. After inactivation, it was dried at a high temperature of 75°C for 2 hours under forced ventilation (wind speed 2.5~3 m / s).

[0061] Then, it is directly subjected to conventional pulverization to obtain Ganoderma lucidum fruiting body powder.

[0062] The rest is the same as in Example 1.

[0063] According to the above method, the sugar content in the Ganoderma lucidum fruiting body powder was 1.12%, and the triterpenoid and sterol content was 0.63%.

[0064] Comparative Example 2: Step b of step 1) in Example 1 is modified as follows: The oxidase was first inactivated using a microwave inactivation method, with the same process conditions as in Example 1. After inactivation, it was subjected to ultra-low temperature treatment in liquid nitrogen at -196°C, thereby instantly forming ice crystals in the Ganoderma lucidum fruiting body. The rest is the same as in Example 1.

[0065] According to the above method, the content of iodine in the ultrafine powder of Ganoderma lucidum fruiting body is 1.03%, and the content of triterpenes and sterols is 0.54%.

[0066] Finally, it should be noted that the above examples are merely some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments and many variations are possible. All variations that can be directly derived or conceived by those skilled in the art from the disclosure of the present invention should be considered within the scope of protection of the present invention.

Claims

1. A method for preparing large honey pills from Ganoderma lucidum fruiting bodies, characterized in that... Includes the following steps: 1) Processing of Ganoderma lucidum fruiting bodies a. Slicing: Select fresh Ganoderma lucidum fruiting bodies, clean them thoroughly, and slice them; b. Divided into the following two stages: The first stage of oxidase inactivation and drying: oxidase was first inactivated by microwave inactivation, and then treated at 75±5℃ for 2±0.5 h under natural ventilation conditions, thereby reducing the water content of the fruiting body. The second stage involves ultra-low temperature treatment with liquid nitrogen at -196℃ to form ice crystals in the fruiting body of Ganoderma lucidum; c. Crushing: The ice crystals of Ganoderma lucidum fruiting bodies are immediately crushed using an ultra-fine pulverizer to obtain ultra-fine powder of Ganoderma lucidum fruiting bodies; 2) Selection and refining of auxiliary materials Honey is used as an auxiliary ingredient; First, boil the bees at 90±1℃ and maintain the temperature for 5±0.5 min; Simmer at 85±1℃ for 15±0.5 min to obtain refined honey; 3) Mixed raw materials Mix the ultrafine powder of Ganoderma lucidum fruiting body obtained in step 1) with the refined honey obtained in step 2) at a mass ratio of 1:(1.5±0.1) and stir evenly. 4) Molding The mixture obtained in step 3) is prepared into pellets to obtain shaped honey pills; 5) Drying and Packaging The shaped honey pills obtained in step 4) are dried at low temperature until the moisture content is ≤15%. Then the dried honey pills are packaged and sealed for storage.

2. The method for preparing Ganoderma lucidum fruiting body honey pills according to claim 1, characterized in that: The microwave inactivation in step 2) is as follows: the microwave generator power is 3~5kW, the frequency is 2450MHz, the inactivation time is 6~8min, and the microwave temperature is 50~70℃.

3. The method for preparing Ganoderma lucidum fruiting body honey pills according to claim 2, characterized in that: The low-temperature drying temperature in step 5) is 40±5℃.

4. The method for preparing large honey pills of Ganoderma lucidum fruiting bodies according to any one of claims 1 to 3, characterized in that: In step 4), the particle size of the formed honey pills is 9±1 mm.

5. Ganoderma lucidum fruiting bodies prepared by any one of the methods described in claims 1 to 4.

Images