Plant extract composition and application thereof

Abstract

The invention provides a plant extract composition which contains oriental cherry extract and roxburgh rose extract, and the weight ratio of solid components of the oriental cherry extract to the roxburgh rose extract is 75: 25-10: 90. The composition disclosed by the invention can be used for remarkably promoting the secretion of anti-aging related protein type I collagen (COL-I) and elastin (Elastin).

Description

TECHNICAL FIELD

[0001] The present application relates to a plant extract, a collagen type I production promoter, and an elastin production promoter. BACKGROUND

[0002] With the improvement of people's living standards and the enhancement of beauty and health awareness, the demand for skin care and anti-aging is increasing. Collagen and elastin, as the key proteins to maintain skin elasticity and firmness, their production and maintenance are crucial to skin health. However, with age and the influence of environmental ultraviolet rays, the body's ability to synthesize collagen and elastin gradually weakens, leading to skin wrinkles, sagging, and other signs of aging. Therefore, finding a method or substance that can effectively promote collagen and elastin production has become a research hotspot in the current beauty and biomedical fields.

[0003] Cherry blossoms, as a symbol of spring, are not only known for their beautiful flowers, but also contain rich bioactive components such as polyphenols and flavonoids in their petals. These components have various biological activities such as antioxidant and anti-inflammatory effects. In recent years, cherry blossom extract has gradually attracted attention in the field of beauty and skin care, and is believed to have the effect of promoting skin metabolism and improving skin quality.

[0004] Rosa roxburghii, also known as wild pear or mountain pear, is a wild fruit tree with a wide distribution. Its fruit contains rich vitamin C, flavonoids, and polyphenols, which have significant antioxidant and anti-inflammatory effects. In traditional medicine, Rosa roxburghii has been used to treat various diseases, including skin diseases. In recent years, research has found that Rosa roxburghii extract can promote the proliferation and differentiation of skin cells, and has a positive effect on improving skin quality and promoting collagen synthesis.

[0005] Although the application of cherry blossom extract and Rosa roxburghii extract in the field of beauty and skin care has achieved certain results, the existing research results still have room for improvement in promoting collagen and elastin production. In order to meet people's pursuit of beauty and health, products with more excellent promotion effect have important practical significance and market value. SUMMARY

[0006] The purpose of the present application is to provide a plant extract composition that can significantly promote collagen and elastin production.

[0007] The present application was completed through repeated research and found that the combination of cherry blossom extract and Rosa roxburghii extract can exhibit a promotion effect on elastin and collagen production that exceeds the use of either alone at the same dose.

[0008] The present application includes the following embodiments.

[0009] [1] A plant extract composition containing cherry blossom extract and roxburgh rose extract, the weight ratio of the solid content of the cherry blossom extract to the roxburgh rose extract being 75:25 to 10:90.

[0010] [2] The composition according to [1], which does not contain other plant extracts.

[0011] [3] Use of the composition according to [1] or [2] in food.

[0012] [4] Use of the composition according to [1] or [2] in cosmetics.

[0013] [5] Use of a composition containing cherry blossom extract and roxburgh rose extract for the preparation of a collagen type I production promoter.

[0014] [6] Use of a composition containing cherry blossom extract and roxburgh rose extract for the preparation of an elastin production promoter.

[0015] The plant extract composition of the present application can be a composition in solid form or a composition in liquid form prepared to a certain concentration.

[0016] Effects of the Invention

[0017] The composition of the present application can significantly promote the secretion of anti-aging related proteins, collagen type I (COL-I) and elastin.

[0018] DETAILED DESCRIPTION OF THE INVENTION

[0019] The plant extract composition of the present application contains cherry blossom extract and roxburgh rose extract, the weight ratio of the solid content of the cherry blossom extract to the roxburgh rose extract being 75:25 to 10:90.

[0020] In the present application, the composition having the above-mentioned constitution can significantly increase the secretion levels of collagen type I and elastin, and in particular, can achieve a promotion level higher than when cherry blossom extract or roxburgh rose extract is used alone.

[0021] In the present application, the cherry blossom extract as an active ingredient can be a water extract or an ethanol extract, and is preferably a water extract. As the cherry blossom extract used in the present application, commercially available cherry blossom extract can be used, or an extract obtained by a known extraction method can be used. For example, an extract prepared by extracting dried cherry blossoms or fresh Prunus serrulata with water or a mixture of water and ethanol can be used.

[0022] Specifically, the following method can be used: prepare dry cherry raw materials, remove the unqualified raw materials such as moldy raw materials, add them to the extraction tank, add pure water, control the mass ratio of water and raw materials to be 50:1-10:1, then heat to 40-80℃ for extraction for 0.5-10 hours. Then filter to remove the residue, obtain the extraction liquid, filter the extraction liquid through a ceramic membrane with a pore size of 50-500 nm, then concentrate through an ultrafiltration membrane, and then freeze-dry to obtain a powder. Alternatively, after concentration, 20-50 mass% of malt dextrin, starch or the like can be added for spray drying to obtain a powder. Here, 20-50 mass% refers to the mass concentration of the auxiliary material in the finished powder after drying.

[0023] In the present application, the Rosa roxburghii Tratt extract as the active ingredient can be a water extract or an ethanol extract, and is preferably a water extract. As the Rosa roxburghii Tratt extract used in the present application, a commercially available Rosa roxburghii Tratt extract or an extract obtained by a known extraction method can be used. For example, an extract can be prepared by juicing fresh Rosa roxburghii Tratt fruits, filtering to remove particulate matter and macromolecular substances, and then concentrating.

[0024] Specifically, the following method can be used: prepare Rosa roxburghii Tratt fresh fruits, wash and juice to obtain Rosa roxburghii Tratt juice, filter the juice through a 100-300 mesh filter cloth, filter the filtrate through a ceramic membrane with a pore size of 50-500 nm to remove particulate matter and macromolecular substances, and then concentrate the obtained filtrate at a low temperature under vacuum at 40-70℃ to obtain an extract containing 20 mass% to 40 mass% of solid matter. Add a certain amount of auxiliary material (such as 20-50 mass% of malt dextrin, starch or the like) to the extract, and then dry using a spray dryer or a freeze dryer to obtain a powder. Here, 20-50 mass% refers to the mass concentration of the auxiliary material in the finished powder after drying.

[0025] As for freeze-drying, the general process is as follows: first, pre-freeze to -40℃ under vacuum, and maintain for several hours, usually 4-6 hours; then, sublimation drying stage, slowly increase the temperature from -40 to -20 to -15℃, maintain for 5-12 hours, then increase the temperature from -20 to -15℃ to -10℃, maintain for 10-24 hours; finally, desorption drying stage, slowly increase the temperature from -10℃ to 30-40℃, maintain for about 10 hours, to obtain the freeze-dried product.

[0026] Then crush, sieve and the like to obtain a finer powder.

[0027] The present inventors have found that the cherry extract and the Rosa roxburghii Tratt extract can produce a synergistic effect in promoting the secretion of elastin and collagen, and in particular, when the cherry extract and the Rosa roxburghii Tratt extract are used in combination at a solid content weight ratio of 90:10 to 10:90, a significant synergistic effect is exhibited.

[0028] In addition, when the weight ratio of the cherry blossom extract to the roxburgh rose extract is 75:25 to 10:90, the synergistic effect is more prominent. Examples

[0029] The technical idea of the present application will be further explained by the following examples. However, the following examples are merely presented to enable those skilled in the art to clearly understand the idea of the present application and are not intended to be a specific limitation on the present application.

[0030] Preparation of raw material extract

[0031] (1) Preparation of cherry blossom extract

[0032] Dry cherry blossom raw material is prepared, and unqualified raw material such as moldy raw material is removed and added to an extraction tank. Pure water is added, and the mass ratio of water to raw material is controlled to be 20:1. Then, the temperature is heated to 50°C, and the extraction time is 1 hour. After that, the residue is removed by filtration to obtain an extraction liquid. The extraction liquid is filtered by a 200 nm ceramic membrane, and then concentrated by an ultrafiltration membrane. Then, 20% malt dextrin is added for spray drying to obtain a powder.

[0033] (2) Preparation of roxburgh rose extract

[0034] Roxburgh rose fresh fruit is prepared, washed, and juiced to obtain roxburgh rose juice. The roxburgh rose juice is filtered by a 200-mesh filter cloth, and the filtrate is filtered by a 200 nm filter ceramic membrane to remove particulate matter and macromolecular substances. The obtained filtrate is vacuum low-temperature concentrated at 60°C to obtain an extract containing 40% by mass of solid. After adding 20% by mass of malt dextrin to the extract, the mixture is dried in a spray dryer to obtain a roxburgh rose extract powder.

[0035] Preparation of composition

[0036] The cherry blossom extract and the roxburgh rose extract powder are weighed and mixed in a certain weight ratio to prepare a composition.

[0037] Example 1

[0038] Cherry blossom extract-roxburgh rose extract composition (cherry blossom-roxburgh rose composition): The cherry blossom extract and the roxburgh rose extract powder are weighed and mixed in a weight ratio of 90:10. A sample solution of a corresponding concentration is prepared using sterile PBS buffer, and the sample is filtered through a 0.22 μm filter membrane before being measured.

[0039] Example 2

[0040] The weight ratio of the cherry blossom extract to the roxburgh rose extract is 75:25, and the same method as in Example 1 is used to obtain a composition solution.

[0041] Example 3

[0042] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 65:35.

[0043] Example 4

[0044] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 60:40.

[0045] Example 5

[0046] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 50:50.

[0047] Example 6

[0048] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 40:60.

[0049] Example 7

[0050] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 35:65.

[0051] Example 8

[0052] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 30:70.

[0053] Example 9

[0054] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 26:74.

[0055] Example 10

[0056] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 25:75.

[0057] Example 11

[0058] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 20:80.

[0059] Example 12

[0060] A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to roxburgh rose extract was 10:90.

[0061] Comparative Example 1

[0062] Using the cherry extract prepared above, without adding the roxburghii extract, a composition solution was obtained in the same manner as in Example 1.

[0063] Comparative Example 2

[0064] Using the roxburghii extract prepared above, without adding the cherry extract, a composition solution was obtained in the same manner as in Example 1.

[0065] <Effect of the composition on the promotion of collagen type I and elastin secretion in the fibroblast model damaged by UVA>

[0066] 1 Test equipment

[0067] Carbon dioxide incubator (Thermo), microplate reader (Tecan), inverted microscope (CEWEI), water bath, vortex shaker, electronic scale, ultraviolet irradiation lamp (Philips), irradiation dose meter (Taimas)

[0068] 2 Materials and reagents

[0069] Fetal bovine serum FBS (Gibco), high-sugar DMEM medium (Gibco), fibroblasts HSF (Chinese Academy of Sciences Cell Bank), double-antibiotic (penicillin + streptomycin, Gibco), complete medium (10% FBS + 90% high-sugar DMEM + 1% double-antibiotic), COL-I detection kit (Elabscience), Elastin detection kit (Elabscience).

[0070] 3 Cell culture

[0071] 3.1 Cell recovery and subculture

[0072] The fibroblasts were taken from liquid nitrogen and quickly placed in a 37℃ water bath to dissolve; then 5ml of preheated medium was added, centrifuged at 1000rpm for 5min to discard the supernatant, and preheated complete medium was added and gently blown to resuspend the cells for culture. Fibroblasts growing to 80-90% were washed twice with PBS, 0.25% trypsin was used for digestion, incubated at 37℃ for 1min, the digestion was terminated with medium, centrifuged at 1000rpm for 5min to discard the supernatant, preheated complete medium was added and gently blown to resuspend the cells for subculture.

[0073] 3.2 Cell culture and inoculation

[0074] The cells were cultured using high-sugar DMEM medium (containing 10% FBS and 1% double-antibiotic), and fibroblasts growing to 80-90% were collected and counted. According to the counting results, the cells were inoculated at a density of 2-8x104 cells / ml were inoculated into 24-well cell culture plates at 500 μl per well.

[0075] 3.3 Administration

[0076] According to the experimental design, administration was performed in groups, and 500 μl was administered per well, and three replicates were designed for each group. Except for the control group, UVA irradiation was performed at a dose of 5 J / cm 2 , and incubation was performed in an incubator (37°C, 5% CO2, 95% RH) for 24 hours.

[0077] 3.4 Result detection

[0078] The cell culture supernatant was centrifuged at 12,000 rpm for 2 minutes, and the supernatant was taken, and the COL-I and Elastin detection kits were used to detect the concentrations of type I collagen (COL-I) and elastin in each sample, respectively.

[0079] Blank control group: a sample that was not subjected to UVA irradiation and was not administered.

[0080] UVA model control group: a sample that was subjected to UVA irradiation but was not administered.

[0081] Administered sample group: a sample that was administered at a concentration of 0.5 mg / ml or 0.1 mg / ml and was subjected to UVA irradiation.

[0082] The UVA model control group showed a significant decrease in the secretion of type I collagen and elastin compared to the control sample due to UVA irradiation.

[0083] The administered sample group showed an increase in the secretion of type I collagen and elastin relative to the UVA model control group, although it was subjected to UVA irradiation, because it was able to stimulate fibroblasts to secrete type I collagen and elastin. Therefore, by calculating the change rate of the protein secretion amount of the administered sample group relative to the UVA model control group, it was possible to evaluate the difference in the effect of the administered composition on stimulating protein secretion.

[0084] The effects of the compositions of each of the examples and comparative examples of the blank control group, the UVA model control group, and the administered sample group on the production of type I collagen and elastin by fibroblasts are shown in Tables 1 and 2 below.

[0085] Table 1 Influence of the blank control group, the UVA model control group, and the administered sample group on the production of type I collagen

[0086]

[0087] Table 2 Influence of the blank control group, the UVA model control group, and the administered sample group on the production of elastin

[0088]

[0089] From the data of the above examples and comparative examples, it can be seen that the control group after UV A irradiation exposure, compared with the blank group without UV A irradiation exposure, the amount of type I collagen and elastin produced by fibroblasts is reduced. The sample group, although exposed to UVA irradiation, after treatment with cherry extract and rose hip extract, produces more type I collagen and elastin than the blank group exposed only to UVA irradiation.

[0090] When the sample group uses cherry extract and rose hip extract in combination, a significant synergistic promotion effect can be observed, that is, when used in combination, it can show a significantly higher promotion effect on the secretion of type I collagen and elastin than when used alone at the same dose. In particular, when the cherry extract and rose hip extract are used in a weight ratio of 75:25 to 10:90, the synergistic effect is more significant.

[0091] It should be noted that in the above determination of the secretion amount of type I collagen and elastin, the concentration of active ingredients (cherry extract and / or rose hip extract) in the fibroblast culture is the concentration used for ex vivo experiments, and is not the concentration of the composition itself. The composition product of the present application can be a solid composition or a liquid composition prepared according to the need for a specific concentration. Those skilled in the art can select appropriate product forms and active ingredient concentrations according to the technical idea of the present application and the actual application needs.

[0092] The composition of the present application can be used for the preparation of type I collagen production promoters, the preparation of elastin production promoters, food use, cosmetic use, etc.

Claims

1. A plant extract composition comprising cherry blossom extract and prickly pear extract, wherein the solid component weight ratio of the cherry blossom extract to the prickly pear extract is 75:25 to 10:

90.

2. The composition according to claim 1, wherein it does not contain any other plant extracts.

3. Use of the composition according to claim 1 or 2 in food.

4. Use of the composition according to claim 1 or 2 in cosmetics.

5. Use of a composition containing cherry blossom extract and prickly pear extract in the preparation of a type I collagen production promoter.

6. Use of a composition containing cherry blossom extract and prickly pear extract in the preparation of an elastin production promoter.

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