A gel dressing for wound care and its preparation method

Abstract

This invention belongs to the field of nursing dressing technology, specifically relating to a gel dressing for wound care and its preparation method. The gel dressing for wound care comprises the following raw materials in parts by weight: 2-10 parts sodium alginate, 1-20 parts gelatin, 1-3 parts nanocomposite, and 1-3 parts glycerin. The nanocomposite is prepared from nWH and GA. During the inflammatory phase of the wound, it preferentially releases zinc ions and gallic acid, rapidly exerting antibacterial and antioxidant effects. In the later healing phase, it releases calcium, phosphorus, and magnesium ions, promoting immune regulation and tissue regeneration. Simultaneously, the nanocomposite combines with the sodium alginate and gelatin hydrogel matrix. The hydrogel network provides a stable carrier for the uniform dispersion of the nanocomposite, and its excellent liquid absorption, water retention, and breathability create an ideal moist healing environment for the wound. The gelatin component further endows the dressing with excellent cell adhesion.

Description

Technical Field

[0001] This invention belongs to the field of nursing dressing technology, specifically relating to a gel dressing for wound care and its preparation method. Background Technology

[0002] In the field of wound care, with the development of medical technology and the increasing demands for wound healing effects, higher standards have been set for the performance of clean antibacterial dressings. Traditional dressings, such as natural gauze, synthetic fiber dressings, foam dressings, and hydrogel dressings, while each with its own characteristics, also have certain drawbacks. Taking natural gauze as an example, it is mainly made of cotton or linen as a base fabric, which is treated with antibacterial or sterilization methods. Although it is convenient to use and has a wide range of applications, it has poor breathability and is prone to adhering to wound tissue, which is not conducive to regular wound maintenance and healing. Its waterproof performance is also not ideal; when it gets wet, a large amount of antibacterial components are lost, and it cannot maintain its antibacterial properties.

[0003] Furthermore, while the excellent absorbency of dressings keeps wounds clean and dry, it also creates conditions for the proliferation of bacteria and other microorganisms. Traditional inorganic antibacterial agents such as silver-loaded dressings can be quite harmful to the human body. Although natural organic antibacterial agents (such as Streptococcus lactis and chitosan) have advantages, they generally do not have broad-spectrum antibacterial activity. Therefore, developing a clean antibacterial dressing for nursing use that can effectively prevent tissue fluid leakage, has minimal irritation to the skin and mucous membranes, and effectively solves problems related to antibacterial components during preparation, as well as its preparation method, has significant practical and clinical value. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a wound care gel dressing and its preparation method. The wound care gel dressing is prepared using sodium alginate and gelatin as a matrix, combined with a specific core-shell structured nanocomposite and glycerin. During the inflammatory phase of a wound, it preferentially releases zinc ions and gallic acid, rapidly exerting antibacterial and antioxidant effects; in the later healing phase, it releases calcium, phosphorus, and magnesium ions, promoting immune regulation and tissue regeneration. Simultaneously, the sodium alginate and gelatin matrix form a gradient structure through a stepwise cross-linking process, balancing the adhesive stability of the dressing with the safety of dressing changes. This avoids the defects of traditional dressings, such as easy detachment or secondary damage during dressing changes, and solves the problems of single function and uncontrollable release of active ingredients in traditional wound dressings, demonstrating significant clinical application value.

[0005] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0006] The present invention provides a gel dressing for wound care, the gel dressing for wound care comprising the following raw materials in parts by weight: 2-10 parts sodium alginate, 1-20 parts gelatin, 1-3 parts nanocomposite, and 1-3 parts glycerin.

[0007] The nanocomposite comprises raw materials in the following mass ratio: Ca(OH)2:Mg(OH)2:GA (gallic acid):Zn(CH3COO)2·2H2O=1-1.5:0.35-0.5:0.17:0.22;

[0008] The preparation method of the nanocomposite includes the following steps:

[0009] A1. Dissolve Ca(OH)2 and Mg(OH)2 in deionized water at 80 °C. The total mass ratio of Ca(OH)2 and Mg(OH)2 to the amount of deionized water is 1.5-2 g:50 mL. Stir for 1 h to obtain a blend. Then, add H3PO4 aqueous solution dropwise to the blend. The volume ratio of H3PO4 aqueous solution to the blend is 0.9-1.1:1. Continue stirring at 80 °C for 12 h, centrifuge for 20 min, and dry for 12 h to obtain nWH (white calcium phosphate).

[0010] A2, take nWH, add it to anhydrous ethanol, the ratio of nWH to anhydrous ethanol is 2g:10mL, sonicate for 30min to obtain suspension I;

[0011] A3. Dissolve Zn(CH3COO)2·2H2O in deionized water at a ratio of 0.11 g: 10 mL to form a zinc source solution. Dissolve GA in anhydrous ethanol at a ratio of 0.17 g: 20 mL. Stir magnetically to form a GA solution. Slowly add the zinc source solution dropwise to suspension I. Continue stirring and sonicating for 30 min. Then add the GA solution at a dropping rate of 1-2 drops / second to form a mixture. Continue stirring magnetically and react at room temperature for 12-24 h to obtain suspension II.

[0012] A4. Centrifuge suspension II, collect the precipitate, wash the precipitate 3-4 times with a mixed solution of anhydrous ethanol and deionized water, and then vacuum dry at 60 °C for 12 h to obtain the nanocomposite.

[0013] This invention also provides a method for preparing a gel dressing for wound care, specifically including the following steps:

[0014] S1, take sodium alginate and gelatin, add them to deionized water at 60-70 ℃, the total mass of sodium alginate and gelatin to the amount of deionized water is 1g:16-20 mL, stir for 2-4 h, then add glycerol, continue stirring for 15 min, cool to room temperature, and get mixture I;

[0015] S2, the nanocomposite was added to deionized water at a ratio of 1 g to 9-15 mL. The mixture was then sonicated for 30 min under ice bath conditions to obtain mixture II.

[0016] S3, under stirring, add mixture II dropwise to mixture I, increase the stirring speed, continue stirring for 1 h, and then vacuum dry for 15-20 min to obtain sol solution;

[0017] S4. Pour the sol solution into a mold, controlling the thickness to 1-5 mm. Spray a 1.0%-3.0% calcium chloride aqueous solution evenly onto the surface for pre-crosslinking. After standing to form a stable epidermis, immerse it in a sufficient amount of calcium chloride solution for overall crosslinking for 1-3 hours to form a solid hydrogel. Rinse the surface of the solid hydrogel with water to remove excess ions. After drying the surface moisture, cut it into the required size, package it, and sterilize it with cobalt-60 source irradiation to obtain a wound care gel dressing.

[0018] Compared with the prior art, the beneficial effects achieved by the present invention are as follows:

[0019] The wound care gel dressing prepared in this invention incorporates a core nanocomposite, achieving a synergistic effect of multiple advantages through structural design and process optimization: the core-shell nanocomposite uses leucobionic calcium phosphate as its core, continuously releasing calcium and phosphorus ions to meet the long-term needs of tissue regeneration, while the outer gallic acid-zinc ion metal-organic framework exerts broad-spectrum antibacterial and antioxidant effects. The two form a synergistic effect of antibacterial, anti-inflammatory, and repair-promoting properties, specifically addressing core issues such as difficult-to-control wound infection and slow healing processes. Simultaneously, this core-shell structure possesses intelligent responsiveness to the wound microenvironment: during the inflammatory phase, i.e., the slightly acidic environment caused by infection, the shell preferentially degrades and releases antibacterial components, Zn... 2+The nanocomposite preferentially releases gallic acid (GA), rapidly inhibiting the spread of infection. As the wound heals and enters the proliferative phase, the environment becomes neutral, and the core gradually becomes exposed. The white phosphorus calcium stone core releases calcium and magnesium ions in an orderly manner to promote tissue repair, achieving on-demand release of functional components, actively regulating the immune microenvironment, and promoting macrophage polarization towards the reparative M2 phenotype, thus achieving highly effective anti-inflammatory effects. The nanocomposite is combined with sodium alginate and gelatin hydrogel matrix. The hydrogel network provides a stable carrier for the uniform dispersion of the nanocomposite, and its excellent liquid absorption, water retention, and air permeability create an ideal moist healing environment for the wound. The gelatin component further endows the dressing with excellent cell adhesion, providing a scaffold for cell migration. At the same time, the uniformly distributed nanocomposite, in turn, enhances the mechanical properties of the hydrogel. The wound care gel dressing prepared by this invention forms a gradient structure with a wear-resistant and leak-proof surface and a breathable and water-retaining interior through a stepwise cross-linking process. This effectively locks in wound exudate to maintain a moist healing environment while reducing the risk of leakage. The addition of gelatin enhances the adhesion between the dressing and the wound, making it less likely to fall off, while glycerin improves the flexibility of the gel, significantly reducing secondary damage to newly formed tissues during dressing changes and significantly improving the patient's nursing experience. Attached Figure Description

[0020] Figure 1 The image shows the healing process of the wound care gel dressing prepared according to the present invention.

[0021] Figure 2 This is a graph showing the antioxidant properties of the wound care gel dressing prepared according to the present invention. Detailed Implementation

[0022] To enable those skilled in the art to better understand the technical solutions of the present invention and to make the above-mentioned features, objectives, and advantages of the present invention clearer and easier to understand, the present invention will be further described below with reference to embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those familiar to those skilled in the art. Furthermore, any methods and materials similar to or equivalent to those described herein may be applied to this invention. The preferred embodiments and materials described herein are for illustrative purposes only and do not limit the scope of this application.

[0024] Unless otherwise specified, all methods described in the following embodiments are conventional. Unless otherwise specified, all materials used in the following embodiments are new materials purchased from the market.

[0025] Example 1: This example provides a wound care gel dressing, which includes the following raw materials in parts by weight: 2 parts sodium alginate, 1 part gelatin, 1 part nanocomposite, and 1 part glycerin;

[0026] The nanocomposite comprises raw materials in the following mass ratio: Ca(OH)2:Mg(OH)2:GA:Zn(CH3COO)2·2H2O=1:0.35:0.17:0.22;

[0027] The preparation method of the nanocomposite includes the following steps:

[0028] A1. Dissolve Ca(OH)2 and Mg(OH)2 in deionized water at 80 °C. The total mass ratio of Ca(OH)2 and Mg(OH)2 to the amount of deionized water is 1.5 g: 50 mL. Stir at 400 rpm for 1 h to obtain a blend. Then, add 0.5 M H3PO4 aqueous solution dropwise to the blend at 12.5 mL / min. The volume ratio of M3PO4 aqueous solution to the blend is 0.9:1. Continue stirring at 80 °C for 12 h. Centrifuge the obtained product at 9500 rpm for 20 min, repeat 3 times. Dry the centrifuged product at 80 °C for 12 h to obtain nWH.

[0029] A2, take nWH, add it to anhydrous ethanol, the ratio of nWH to anhydrous ethanol is 2 g: 10 mL, sonicate for 30 min to obtain suspension I;

[0030] A3. Dissolve Zn(CH3COO)2·2H2O in deionized water at a ratio of 0.11 g: 10 mL to form a zinc source solution. Dissolve GA in anhydrous ethanol at a ratio of 0.17 g: 20 mL. Stir magnetically to form a GA solution. Slowly add the zinc source solution dropwise to suspension I. Continue stirring and sonicating for 30 min. Then add the GA solution at a dropping rate of 1 drop / second to form a mixture. Continue stirring magnetically and react at room temperature for 12 h to obtain suspension II.

[0031] A4. Centrifuge suspension II, collect the precipitate, wash the precipitate three times with a mixed solution of anhydrous ethanol and deionized water, and then vacuum dry at 60 °C for 12 h to obtain the nanocomposite.

[0032] This embodiment also provides a method for preparing a gel dressing for wound care, specifically including the following steps:

[0033] S1, take sodium alginate and gelatin, add them to deionized water at 60 ℃, the total mass of sodium alginate and gelatin to the amount of deionized water is 1g:16 mL, stir for 2 h, then add glycerin, continue stirring for 15 min, cool to room temperature, and obtain mixture I;

[0034] S2, the nanocomposite was added to deionized water at a ratio of 1 g to 9 mL, and the mixture was sonicated for 30 min under ice bath conditions to obtain mixture II.

[0035] S3, under continuous stirring at 200 rpm, mixture II was added dropwise to mixture I, the stirring speed was increased to 400 rpm, and stirring was continued for 1 h. Then, the mixture was vacuum dried and treated under vacuum at -0.1 MPa for 15 min to obtain a sol solution.

[0036] S4. The sol solution is poured into a mold, with a thickness of 1 mm. A 1.0% calcium chloride aqueous solution is sprayed evenly on the surface for pre-crosslinking. After standing to form a stable epidermis, it is immersed in a sufficient amount of calcium chloride solution for overall crosslinking for 1 h to form a solid hydrogel. The surface of the solid hydrogel is rinsed with water to remove excess ions. After drying the surface moisture, it is cut, packaged, and sterilized by cobalt-60 irradiation with an irradiation dose of 15 kGy to obtain a wound care gel dressing.

[0037] Example 2: This example provides a wound care gel dressing, which includes the following raw materials in parts by weight: 4 parts sodium alginate, 2 parts gelatin, 2 parts nanocomposite, and 2 parts glycerin;

[0038] The nanocomposite comprises raw materials in the following mass ratio: Ca(OH)2:Mg(OH)2:GA:Zn(CH3COO)2·2H2O=1.5:0.4:0.17:0.22;

[0039] The preparation method of the nanocomposite includes the following steps:

[0040] A1. Dissolve Ca(OH)2 and Mg(OH)2 in deionized water at 80 °C. The total mass ratio of Ca(OH)2 and Mg(OH)2 to the volume of deionized water is 2 g: 50 mL. Stir at 400 rpm for 1 h to obtain a blend. Then, add 0.5 M H3PO4 aqueous solution dropwise to the blend at 12.5 mL / min. The volume ratio of H3PO4 aqueous solution to the blend is 1:1. Continue stirring at 80 °C for 12 h. Centrifuge the obtained product at 9500 rpm for 20 min, repeat 3 times. Dry the centrifuged product at 80 °C for 12 h to obtain nWH.

[0041] A2, take nWH, add it to anhydrous ethanol, the ratio of nWH to anhydrous ethanol is 2g:10mL, sonicate for 30min to obtain suspension I;

[0042] A3. Dissolve Zn(CH3COO)2·2H2O in deionized water at a ratio of 0.11 g: 10 mL to form a zinc source solution. Dissolve GA in anhydrous ethanol at a ratio of 0.17 g: 20 mL. Stir magnetically to form a GA solution. Slowly add the zinc source solution to suspension I. Continue stirring and sonicating for 30 min. Then add the GA solution at a rate of 2 drops / second to form a mixture. Continue stirring magnetically and react at room temperature for 24 h to obtain suspension II.

[0043] A4. Centrifuge suspension II, collect the precipitate, wash the precipitate four times with a mixed solution of anhydrous ethanol and deionized water, and then vacuum dry at 60 °C for 12 h to obtain the nanocomposite.

[0044] This embodiment also provides a method for preparing a gel dressing for wound care, specifically including the following steps:

[0045] S1, take sodium alginate and gelatin, add them to deionized water at 65 ℃, the total mass of sodium alginate and gelatin to the amount of deionized water is 1g:18 mL, stir for 2-4 h, then add glycerin, continue stirring for 15 min, cool to room temperature, and get mixture I;

[0046] S2, the nanocomposite was added to deionized water at a ratio of 1 g to 12 mL. The mixture was then sonicated for 30 min under ice bath conditions to obtain mixture II.

[0047] S3, under continuous stirring at 300 rpm, add mixture II dropwise to mixture I, increase the stirring speed to 500 rpm, continue stirring for 1 h, then vacuum dry, and treat with vacuum to -0.1 MPa for 20 min to obtain sol solution;

[0048] S4. Pour the sol solution into a mold, controlling the thickness to 3 mm. Spray a 2.0% calcium chloride aqueous solution evenly onto the surface for pre-crosslinking. After standing to form a stable epidermis, immerse it in a sufficient amount of calcium chloride solution for overall crosslinking for 1-3 hours to form a solid hydrogel. Rinse the surface of the solid hydrogel with water to remove excess ions. After drying the surface moisture, cut it, package it, and sterilize it by irradiation with a cobalt-60 source at a dose of 20 kGy to obtain a gel dressing for wound care.

[0049] Example 3: This example provides a wound care gel dressing, which includes the following raw materials in parts by weight: 10 parts sodium alginate, 20 parts gelatin, 3 parts nanocomposite, and 3 parts glycerin;

[0050] The nanocomposite comprises raw materials in the following mass ratio: Ca(OH)2:Mg(OH)2:GA:Zn(CH3COO)2·2H2O=1.5:0.5:0.17:0.22;

[0051] The preparation method of the nanocomposite includes the following steps:

[0052] A1. Dissolve Ca(OH)2 and Mg(OH)2 in deionized water at 80 °C. The total mass ratio of Ca(OH)2 and Mg(OH)2 to the volume of deionized water is 2 g: 50 mL. Stir at 400 rpm for 1 h to obtain a blend. Then, add 0.5 M H3PO4 aqueous solution dropwise to the blend at 12.5 mL / min. The volume ratio of H3PO4 aqueous solution to the blend is 1.1:1. Continue stirring at 80 °C for 12 h. Centrifuge the obtained product at 9500 rpm for 20 min, repeat 3 times. Dry the centrifuged product at 80 °C for 12 h to obtain nWH.

[0053] A2, take nWH, add it to anhydrous ethanol, the ratio of nWH to anhydrous ethanol is 2g:10mL, sonicate for 30min to obtain suspension I;

[0054] A3. Dissolve Zn(CH3COO)2·2H2O in deionized water at a ratio of 0.11 g: 10 mL to form a zinc source solution. Dissolve GA in anhydrous ethanol at a ratio of 0.17 g: 20 mL. Stir magnetically to form a GA solution. Slowly add the zinc source solution to suspension I. Continue stirring and sonicating for 30 min. Then add the GA solution at a rate of 2 drops / second to form a mixture. Continue stirring magnetically and react at room temperature for 24 h to obtain suspension II.

[0055] A4. Centrifuge suspension II, collect the precipitate, wash the precipitate four times with a mixed solution of anhydrous ethanol and deionized water, and then vacuum dry at 60 °C for 12 h to obtain the nanocomposite.

[0056] This embodiment also provides a method for preparing a gel dressing for wound care, specifically including the following steps:

[0057] S1, take sodium alginate and gelatin, add them to deionized water at 70 ℃, the total mass of sodium alginate and gelatin to the amount of deionized water is 1g:20 mL, stir for 4 h, then add glycerin, continue stirring for 15 min, cool to room temperature, and get mixture I;

[0058] S2, the nanocomposite was added to deionized water at a ratio of 1 g to 15 mL. The mixture was then sonicated for 30 min under ice bath conditions to obtain mixture II.

[0059] S3, under continuous stirring at 400 rpm, mixture II was added dropwise to mixture I, the stirring speed was increased to 600 rpm, and stirring was continued for 1 h. Then, the mixture was vacuum dried and treated under vacuum at -0.1 MPa for 20 min to obtain a sol solution.

[0060] S4. The sol solution is poured into a mold, with a thickness of 5 mm. A 3.0% calcium chloride aqueous solution is sprayed evenly on the surface for pre-crosslinking. After standing to form a stable epidermis, it is immersed in a sufficient amount of calcium chloride solution for overall crosslinking for 3 hours to form a solid hydrogel. The surface of the solid hydrogel is rinsed with water to remove excess ions. After drying the surface moisture, it is cut into the required size, packaged, and sterilized by cobalt-60 irradiation with an irradiation dose of 25 kGy to obtain a wound care gel dressing.

[0061] The difference between Comparative Example 1 and Example 2 is that no nanocomposite was added; the rest of the parts are exactly the same as Example 2.

[0062] The difference between Comparative Example 2 and Example 2 is that nWH is not added; the rest is exactly the same as Example 2.

[0063] The difference between Comparative Example 3 and Example 2 is that the nanocomposite is replaced with ZnO, and the rest is exactly the same as Example 2.

[0064] Experimental Example 1: Adhesion Performance: Using the wound care gel dressings prepared in Examples 1-3 and Comparative Examples 1-3 as samples, the adhesion performance of the samples on various substrate surfaces (polytetrafluoroethylene (PTFE), wood, glass, pigskin, pig bone, pig heart, pig liver, and pork) was tested on a tensile testing machine at a loading rate of 50 mm / min and a force of 100 N. In short, the sample was uniformly applied to the surface of these substrates, compressed under a fixed normal load of 100 N for 3 min, and then subjected to an lap shear adhesion test. The adhesive strength of the hydrogel can be calculated using the following formula: σ = F max / S,F max The maximum force measured during the lap shear adhesion test is represented by S, where S is the contact area. Each test was repeated three times, and the adhesion strength results on pigskin are recorded in Table 1.

[0065] Experiment Example 2: In vitro antibacterial performance test: The wound care gel dressings prepared in Examples 1-3 and Comparative Examples 1-3 were used as test samples. The antibacterial performance of the test samples was tested using Staphylococcus aureus and Escherichia coli: (1) 100 μL of bacterial suspension and 900 μL of sterile PBS hydrogel extract were added to a 24-well plate, with 900 μL of sterile PBS solution as the control group. Co-cultured at 37℃ for 1 h; (2) The bacterial suspension co-cultured with hydrogel was evenly spread on an agar plate by the coating method, and cultured at 37℃ for another 24 h; (3) The agar plate was removed, and the colonies were counted and photographed. Each group of experiments was repeated 3 times. The relative survival rate of bacteria was calculated using the following formula: relative bacterial activity (%) = A / B × 100%, where A is the number of colonies in the experimental group and B is the number of colonies in the control group. Each group of experiments was repeated 3 times, and the results are recorded in Table 1.

[0066] Experimental Example 3: Hemolysis Rate Test: Using the wound care gel dressing prepared in Example 2 of this invention as the sample, 1.5 mL of mouse blood was collected and centrifuged at 1500 rpm / min for 5 min to obtain red blood cells. The red blood cells were then washed with PBS (pH 7.4) solution three times and diluted to 5% (V / V) to obtain a red blood cell solution. A sample (5 mm in diameter, 1 mm in thickness) was added to 2 mL of the red blood cell solution and incubated at 37 ℃ at 100 rpm / min for 1 h. Six parallel samples were set up for each group. After incubation, the sample was centrifuged at 1500 rpm / min for 5 min. 200 μL of the suspension was added to a 96-well plate. The absorbance of the solution at 540 nm was measured using UV-vis. The hemolysis rate was calculated using the following formula: Hemolysis rate (%) = (Am-Ap) / (An-Ap) × 100; where, A... m A represents the absorbance of hydrogel groups with different concentrations. n and A p The absorbance values ​​of the positive control group (water) and the negative control group (PBS) are shown in Table 1.

[0067] Table 1: Performance Test Results

[0068]

[0069] Table 1 shows that the bonding strength of Examples 1-3 was significantly higher than that of Comparative Examples 1 and 3, but slightly lower than that of Comparative Example 2. This indicates that the wound care gel dressing prepared by the present invention retains the excellent adhesiveness of GA and can adhere firmly to the skin surface. Regarding antibacterial properties, Examples 1-3 of the present invention exhibited excellent antibacterial performance, with significantly higher antibacterial effects than Comparative Examples 1-3. This suggests that the introduction of nWH and the GA / Zn... 2+It produced a strong synergistic antibacterial effect; the hemolysis rate results showed that it effectively controlled Zn. 2+ The release behavior of GA avoids the impact on the red blood cell membrane caused by instantaneous high concentration release.

[0070] Example 4: Antioxidant Performance Test: Using the wound care gel dressings prepared in Examples 1-3 and Comparative Examples 1-3 as samples, the antioxidant performance of the samples was tested using 1,1-diphenyl-2-picrylhydrazine (DPPH) as a free radical. The samples (0.001%, 0.0025%, 0.005%, 0.0075%, 0.01%, and 0.015% (W / V)) were mixed with a DPPH ethanol solution (0.050% (W / V)). After incubation in the dark for 0, 20, 40, and 60 min, the absorbance of the reaction solution at 516 nm was measured using UV-vis, and the DPPH scavenging efficiency of each sample was calculated using the following formula: DPPH scavenging efficiency (%) = (A0 - A S ) / A0×100; where A0 is the blank absorbance (DPPH + ethanol), A S The absorbance of the sample (DPPH + ethanol + sample) is shown in the following figure. Figure 2 As shown.

[0071] Figure 1 The results showed that on day 3, both groups of wounds exhibited varying degrees of inflammatory response. On day 7, Example 2 showed abundant granulation tissue and neovascularization. On day 14, both the control group and Example 2 showed granulation tissue and neovascularization, indicating that wound repair had entered the remodeling stage. Compared with the control group, the newly formed tissue in Example 2 was neatly arranged and the stratum corneum was thinner, indicating that the wound care gel dressing prepared in this invention has good wound healing ability. Figure 2 The wound care gel dressings prepared in Examples 1-3 of this invention can effectively remove free radicals generated at the wound site and accelerate wound healing.

[0072] In summary, the wound care gel dressing of this invention achieves a high degree of synergy between structure and function through scientific raw material formulation and precise preparation process. The nanocomposite with nWH as the core and gallic acid-zinc ion metal-organic framework as the shell precisely adapts to the natural process of wound healing; the sodium alginate and gelatin hydrogel matrix provide a stable carrier, possessing excellent liquid absorption and water retention, breathability, and bioadhesion. Combined with the gradient structure formed by the stepwise cross-linking process, it ensures stable adhesion while reducing damage during dressing changes. Experimental data further validates that this dressing exhibits outstanding adhesion strength and antibacterial properties, and the synergistic effect of the nanocomposite and its components is significantly superior to traditional dressings. In conclusion, this dressing effectively overcomes the bottlenecks of traditional products, such as single function and uncontrollable release, demonstrating significant advantages and clinical translational potential in the care of chronic, difficult-to-heal wounds.

[0073] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A gel dressing for wound care, characterized in that, The raw materials include the following components by weight: 2-10 parts of sodium alginate, 1-20 parts of gelatin, 1-3 parts of nanocomposite, and 1-3 parts of glycerol; The nanocomposite includes the following raw materials in the following mass ratio: Ca(OH)2:Mg(OH)2:gallic acid:Zn(CH3COO)2·2H2O = 1-1.5:0.35-0.5:0.17:0.22; The preparation method of the nanocomposite includes the following steps: A1, dissolving Ca(OH)2 and Mg(OH)2 in deionized water, stirring to obtain a blending liquid, then adding H3PO4 aqueous solution dropwise into the blending liquid, and continuing to stir, then centrifuging, drying to obtain white whitlockite; A2, taking the white whitlockite, adding anhydrous ethanol, and ultrasonic treatment to obtain a suspension I; A3, dissolving Zn(CH3COO)2·2H2O in deionized water to form a zinc source solution, dissolving gallic acid in anhydrous ethanol, magnetically stirring to form a gallic acid solution, slowly adding the zinc source solution into the suspension I, continuing to stir and ultrasonic, then adding the gallic acid solution to form a mixed solution, continuing to magnetically stir, and reacting at room temperature to obtain a suspension II; A4, centrifuging the suspension II, collecting the precipitate, washing, and vacuum drying to obtain the nanocomposite.

2. A gel dressing for the management of wounds according to claim 1, wherein In step A1, the total mass of Ca(OH)2 and Mg(OH)2 is 1.5-2 g, and the amount of deionized water is 50 mL. In step A2, the amount of white whitlockite is 2 g, and the amount of anhydrous ethanol is 10 mL.

3. The gel dressing for use in the care of wounds according to claim 1, characterized in that, In step A3, the amount of Zn(CH3COO)2·2H2O is 0.11 g, the amount of deionized water is 10 mL, the amount of gallic acid is 0.17 g, and the amount of anhydrous ethanol is 20 mL.

4. A process for the preparation of a gel dressing for the treatment of wounds according to any one of claims 1 to 3, characterized in that, Specifically includes the following steps: S1, taking sodium alginate and gelatin, adding deionized water, stirring, adding glycerol, continuing to stir, cooling to room temperature, and obtaining a mixture I; S2, adding the nanocomposite into deionized water, ultrasonic treatment under ice bath conditions, and obtaining a mixture II; S3, adding the mixture II dropwise into the mixture I under continuous stirring, continuing to stir, and then vacuum drying to obtain a sol solution; S4, pouring the sol solution into a mold, performing pre-crosslinking treatment to form a solid hydrogel, washing, cutting, sterilizing, and obtaining a gel dressing for wound care.

5. The method of claim 4, wherein the gel dressing is prepared by the steps of: In step S1, the total mass of sodium alginate and gelatin is 1 g, and the amount of deionized water is 16-20 mL. In step S2, the amount of nanocomposite is 1 g, and the amount of deionized water is 9-15 mL.

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