Method and device for screening sensitive light sources of adult fishes
By designing a method and device for screening sensitive light sources for adult fish, the problem of unreasonable screening of sensitive light environments for adult fish in existing technologies has been solved, the stability and repeatability of experimental results have been achieved, and the pass rate of fish passage facilities has been improved.
Patent Information
- Application Number
- CN202511343206.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-19
- Publication Date
- 2025-11-28
AI Technical Summary
Existing experimental methods and devices lack representativeness and cannot effectively screen the sensitive light environment of adult fish. This leads to unreasonable design of fish passage facilities, affecting fish safety and reproductive migration. Furthermore, unreasonable selection and placement of light sources result in uneven light intensity distribution and an inability to precisely control water flow speed, thus affecting experimental results.
A method and device for screening sensitive light sources for adult fish were designed. The method involves adjusting the spectral color using filter paper, measuring the light environment with an illuminance meter, monitoring fish behavior via video, setting up a control experiment, and screening out the sensitive light environment for adult fish. The device includes a quick-switching monitoring mechanism, a filter switching mechanism, and an adjustment mechanism to ensure the uniformity and controllability of the light source and water flow.
This method enables the selection of sensitive light environments for adult fish through a single experiment, accurately reflecting fish responses, reducing light path interference and water flow unevenness, ensuring the stability and repeatability of experimental results, adapting to different lighting modes and water flow conditions, and improving the throughput of fish passage facilities.
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Figure CN121014569A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of fish behavior, in particular to a method and device for screening light sources sensitive to fish. BACKGROUND
[0002] Fish behavior is complexly regulated by a variety of biological and non-biological factors, among which environmental factors, especially light conditions, play a key role. According to the differences in the behavioral responses of fish to light stimulation, fish can be divided into two categories: positive phototaxis and negative phototaxis. As an important environmental signal, light conditions affect the key behavioral patterns of fish such as feeding, reproduction, and migration through direct photobiological effects and indirect ecological chain effects. Specifically, changes in parameters such as light intensity, spectral composition, and photoperiod can significantly regulate the phototactic behavior, clustering characteristics, and circadian rhythms of fish.
[0003] Fish are easily damaged or killed by friction and collision with the blades of the water turbine when passing through the water turbine, and many fish are easily attracted to the spillway by the water flow when the dam is releasing water. These not only have a certain impact on the normal operation of the equipment, but also cause the death of fish, and even lead to the extinction of some rare fish species. Therefore, when building a dam, the negative phototaxis of fish can be used to block fish from entering the water turbine power generation port and the spillway water inlet. In order to effectively protect aquatic biological resources and maintain river ecological connectivity, fish passing facilities are usually built to protect the fish migration channel during the construction of water conservancy projects. Studies have shown that based on the phototactic behavior characteristics of fish, optimizing the light conditions (such as the arrangement of light sources with specific wavelengths and intensities) at the entrance area of fish passing facilities can significantly improve the induction efficiency of fish, thereby improving the overall passing rate of fish passing facilities. Therefore, carrying out fish light sensitivity experiments and systematically studying the effects of different light parameters (such as light color and intensity) on the behavior of target fish have important scientific guiding significance for the optimization design of the light environment of fish passing facilities. The current experimental methods and experimental devices have the following defects:
[0004] 1. The experiment is not representative. Existing researches are mostly limited to the observation of the phototactic behavior of juvenile fish or immature individuals. However, fish passing facilities designed based on incomplete behavioral data may not effectively guide adult fish, resulting in damage to adult fish or their inability to identify the entrance of fish passing facilities. This failure of protection is particularly deadly to rare fish species (such as Chinese sturgeon and Yangtze sturgeon) that need to complete reproductive migration, as their inability to reach the spawning ground will directly lead to reproductive failure, which may eventually lead to irreversible population decline or even regional extinction.
[0005] 2. The light source selection and placement position is unreasonable. The light source selected in the past indoor experiment is mostly ordinary lamp tube or halide lamp, the light intensity distribution is extremely uneven, the light intensity range is not controllable, the experiment working condition that can be set is limited, the accurate wavelength and light intensity range required by the experiment cannot be obtained, and the light source placement position is only placed at one end of the water tank, which cannot effectively determine whether it is sensitive to the light environment.
[0006] 3. The water flow speed cannot be accurately controlled. In the past research, the circulating pump is directly used to pump water to create a flow field, and the water flow is directly controlled by the water pump, which will make the flow field distribution uneven, and vortex and drop flow are easily generated at the water inlet of the experimental device, which seriously affects the experimental results.
[0007] Therefore, a method and device for screening sensitive light sources of adult fish are proposed to solve the above problems. SUMMARY
[0008] Therefore, the technical problem to be solved by the present application is to provide a method and device for screening sensitive light environment experiment of adult fish. The sensitivity and avoidance of adult fish to a certain light environment can be inferred by one experiment, so that the sensitive light environment of adult fish is screened. It is beneficial to the indoor research and practical engineering application of light induction and fish driving technology
[0009] To achieve the above purpose, the present application provides the following technical scheme: a method and device for screening sensitive light sources of adult fish, comprising:
[0010] S1, adjust the filter paper at both ends of the water tank, switch the filter paper by rotating the middle part of the adjusting shaft, and create a specific light spectrum color light environment to be tested;
[0011] S2, turn on the light source module, and measure the light environment of the water tank with an illuminometer to obtain its distribution range; and take the dark lightless environment as a control group;
[0012] S3, according to the fish body type, finely adjust the adjusting mechanism to make the size of the water tank more suitable for the movement of the experimental fish, put the experimental fish into the water tank, adjust the water flow control assembly to make it become a still water environment, and then turn on the video monitoring system after the fish adapt to it, and observe and record the behavior of the experimental fish swimming freely in the water tank;
[0013] S4, set up a control experiment group, and perform a control experiment on the fish in a dark lightless environment to exclude the system error of the experiment;
[0014] S5, perform a light environment experiment on the fish to be tested: in the same time period as the free swimming behavior experiment, perform a light environment experiment on the fish to be tested, switch different filter papers for filtering, set the behavior and swimming speed of the fish under different light environment stimulation, analyze and determine the phototactic properties of the fish, and thus screen out positive phototaxis and negative phototaxis respectively.
[0015] As preferred, in S4, when the environment is dark, the two light source modules at the ends of the water tank are respectively set as the first light source and the second light source. When the first light source near the experimental fish at one end of the water tank is turned on, the light intensity of the first light source is reduced to 0 lx when the experimental fish swims through the middle of the water tank, and the second light source at the other end of the water tank is turned on at the same time. When the experimental fish swims through the middle of the water tank again and swims towards the first light source device, the second light source device is turned off, the first light source device is turned on, and the cycle is repeated. The light source is turned on for 20 minutes or until the experimental fish no longer responds.
[0016] As preferred, in S5, when the fish is phototaxis, the first light source device at the end of the water tank away from the experimental fish is turned on. When the experimental fish swims towards the light source and through the middle of the water tank, the light intensity of the first light source device is reduced to 0 lx, and the second light source device at the other end of the water tank is turned on at the same time. When the experimental fish exhibits positive phototaxis to the test light environment and turns around to swim towards the second light source device, the second light source device is turned off, and the first light source device is turned on again. The cycle is repeated for 20 minutes or until the experimental fish no longer responds.
[0017] A device for screening light sources sensitive to fish, comprising a water tank, the water tank is symmetrically arranged, the bottom of the water tank is provided with a water flow tank, the bottom of the water flow tank is provided with a support column, and the two ends of the water tank are provided with light source modules. The device further comprises a quick switching monitoring mechanism, a light filtering switching mechanism and an adjusting mechanism.
[0018] The quick switching monitoring mechanism is arranged in the middle of the symmetrically arranged water tank, and is used for adjusting the switching of the light source modules.
[0019] The light filtering switching mechanism is arranged on the outer surface of the water tank, comprising a light filtering paper, the light filtering paper is arranged on the outer surface of the two ends of the water tank, and the light filtering switching mechanism is used for adjusting the light filtering paper.
[0020] The adjusting mechanism is arranged in the water tank, and is used for adjusting the internal volume of the water tank and controlling the water flow.
[0021] As preferred, the quick switching monitoring mechanism comprises a transmission shaft, the transmission shaft is symmetrically rotatably arranged at the two ends of the water tank, a first transmission belt is transmissionally connected to the outer surface of the middle of the transmission shaft, a fixed plate is fixedly arranged on the first transmission belt in a symmetrical manner, a folding plate is fixedly arranged on the fixed plate, the folding plate is fixedly arranged on the light source module away from the fixed plate, a positioning device is arranged on the light source module away from the folding plate, a detection device is arranged on the outer surface of the water tank near the positioning device, and the positioning device and the detection device are electrically connected.
[0022] Preferably, the filter switching mechanism comprises adjusting shafts symmetrically arranged at both ends of the sink, outer surfaces of both ends of the adjusting shafts are rotatably mounted on the sink, both ends of the filter paper are wound around the middle part of the adjusting shaft, a belt pulley is fixedly mounted on the outer surface of one end of the adjusting shaft, a second transmission belt is drivingly mounted on the belt pulley, a damping wheel is mounted on the side of the sink close to the belt pulley, the second transmission belt is drivingly connected to the outer surface of the damping wheel away from the adjusting shaft, the middle part of both ends of the sink is made of light-transmitting glass, and the inner side of the filter paper slides on the side of the light-transmitting glass away from the middle part of the sink.
[0023] Preferably, the adjusting mechanism comprises an adjusting ring, a clamping shaft is fixedly mounted on the middle part of the adjusting ring, clamping grooves are uniformly formed on the clamping shaft, L-shaped pawls are clamped in the clamping grooves, a rotating shaft is rotatably connected to the middle part corner of the L-shaped pawl, a torsional spring is mounted on the rotating shaft, one end of the torsional spring is fixedly mounted on the sink, the other end of the torsional spring is fixedly mounted on the L-shaped pawl, a gear is fixedly connected to the end of the clamping shaft away from the adjusting ring, slide racks are symmetrically engaged with the gear teeth surfaces on both sides of the gear, the slide racks are slidingly mounted on the sink, a baffle is fixedly connected to the end of the slide rack away from the gear, the baffle slides in the sink, guide blocks are slidingly connected to the inner walls of both sides of the sink, return springs are sleeved on the outer surfaces of the guide blocks, one end of the return spring is fixedly connected to the guide block, the other end of the return spring is fixedly mounted on the sink, the end of the guide block close to the middle part of the sink is fixedly connected to the baffle, and a waterproof sealing strip is arranged at the contact point between the baffle and the inner wall of the sink.
[0024] Preferably, the adjusting mechanism further comprises a pressure increasing device, the pressure increasing device is mounted on the middle part of the sink, a spray head is mounted on the pressure increasing device, the spray head is fixedly mounted on the sink, the bottom right side of the sink is made of an inclined slope surface with a slope of 0-2 degrees, adjusting plates are rotatably mounted on the slope surface of the bottom of the sink, adjusting teeth are fixedly mounted on the outer surface of one end of the adjusting plate, the adjusting teeth are engaged with pull racks, the pull racks are slidingly mounted on the slope surface of the bottom of the sink, a threaded adjusting rod is rotatably mounted on the end of the pull rack away from the pressure increasing device, the threaded adjusting rod is threadedly mounted on the inner wall of the sink away from the pull rack, and a waterproof sealing gasket is installed outside the threaded connection between the pull rack and the threaded adjusting rod, for sealing the inside of the sink.
[0025] Preferably, the sink is made of opaque material, and the inner surface is coated with a black matt layer.
[0026] Compared with the prior art, the method and device for screening sensitive light sources of fish provided by the application have the following beneficial effects:
[0027] 1. The present application can analyze and judge the innate sensitivity and avoidance of adult fish to certain light environment through one experiment, thereby screening out the sensitive light environment of adult fish, which is beneficial to the experimental research of light attraction and fish driving technology and the rapid development of experiments in practical engineering.
[0028] 2. The present application does not need to rely on feed or bait for long-term domestication, and the verified sensitivity of adult fish to different light environments truly reflects the real natural reaction of fish, and the experimental results are clear.
[0029] 3. Compared with the filter paper in the prior art which needs to be adhered to the surface of the light source lens with glue, the present application is designed in a non-contact manner, and the filter film is installed between the light source and the experimental tank through an independent support or a winding mechanism, so that the light source lens is not contacted at all, and the risk of glue residue, scratches or pollution is completely eliminated; the original transmittance and light field distribution of the light source lens are maintained for a long time, and the stability and repeatability of the experimental light conditions are ensured.
[0030] 4. Through the adjustment mechanism setting, the core of the light detection is light stimulation, and the attenuation of light in water is closely related to water depth. For researches that require specific light intensity to reach the eyes or body of fish, accurate control of water depth is crucial. The adjustable volume allows researchers to set the water depth that best meets the experimental requirements, ensuring the consistency of light parameters between experimental groups or different experiments.
[0031] (1) Reduce light path interference: at a shallow water depth, the absorption and scattering of water to light can be reduced, making the light stimulation closer to the set value, especially in experiments that require precise control of light intensity.
[0032] (2) Adapt to different light modes: some experiments may require a vertical light gradient, and adjustable water depth makes it easier to create and maintain such a gradient.
[0033] (3) Water flow uniformity control: by adjusting the angle of the adjustment plate, the flow speed of the water flow can be controlled, so that different water flow environments can be created in the tank to better simulate the water flow conditions in the real environment, which is beneficial to the accuracy of the test, and at the same time meets the adjustable control of different water flows. BRIEF DESCRIPTION OF DRAWINGS
[0034] Figure 1 is a schematic diagram of the three-dimensional structure of the present application;
[0035] Figure 2 is an auxiliary schematic diagram of the three-dimensional structure of the present application;
[0036] Figure 3 is a schematic diagram of the structural connection relationship of the light filter switching mechanism of the present application;
[0037] Figure 4 For the present application Figure 3 Enlarged view of A in the present application;
[0038] Figure 5 For the present application adjustment mechanism structure connection relationship auxiliary schematic diagram;
[0039] Figure 6 For the present application Figure 5 Enlarged view of B in the present application;
[0040] Figure 7 For the present application water flow regulation assembly structure connection relationship schematic diagram;
[0041] Figure 8 For the present application Figure 7 Enlarged view of C in the present application;
[0042] Figure 9 For the present application water flow regulation assembly structure connection relationship auxiliary schematic diagram.
[0043] In the figure:
[0044] 1, sink; 11, support; 12, light source module; 13, water flow tank;
[0045] 2, quick switching monitoring mechanism; 21, transmission shaft; 22, first transmission belt; 23, fixed plate; 24, folding plate; 25, positioning device; 26, detection device;
[0046] 3, light filter switching mechanism; 31, light filter paper; 32, adjustment shaft; 33, pulley; 34, second transmission belt; 35, damping wheel;
[0047] 4, adjustment mechanism; 41, adjustment ring; 42, clamping shaft; 43, L-shaped pawl; 44, gear; 45, sliding rack; 46, baffle; 47, guide block; 48, return spring;
[0048] 5, water flow regulation assembly; 51, booster device; 52, spray head; 53, adjustment plate; 54, adjustment tooth; 55, pull rack; 56, threaded adjustment rod. DETAILED DESCRIPTION
[0049] The technical solutions of the embodiments of the present application will be described in detail below with reference to the accompanying drawings of the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.
[0050] The present application will be further described in detail below according to the drawings and embodiments.
[0051] Example 1, please refer to Figures 1 to 9 As shown:
[0052] To address the problems mentioned in the technical solutions, this application provides a device for screening sensitive light sources for adult fish, including a water tank 1, the water tanks 1 being symmetrically arranged, a water flow channel 13 being installed at the bottom of the water tank 1, a support column 11 being installed at the bottom of the water flow channel 13, and light source modules 12 being arranged at both ends of the water tank 1. It also includes a quick-switching monitoring mechanism 2, a filter switching mechanism 3, and an adjustment mechanism 4.
[0053] The quick-switching monitoring mechanism 2 is set in the middle of the water tank 1 in a symmetrical arrangement. The quick-switching monitoring mechanism 2 is used to adjust the switching of the light source module 12.
[0054] The filter switching mechanism 3 is disposed on the outer surface of the water tank 1, and includes filter paper 31. The filter paper 31 is disposed on the outer surfaces of both ends of the water tank 1. The filter switching mechanism 3 is used to adjust the filter paper 31.
[0055] The regulating mechanism 4 is installed in the water tank 1. The regulating mechanism 4 is used to regulate the internal volume of the water tank 1 and control the water flow.
[0056] The water tank 1 is made of opaque material with a black matte coating on the inner surface. The dimensions of the water tank 1 range from 7-10m in length, 2-5m in width, and 1-2m in height. The preferred dimensions in this scheme are 8m in length, 3m in width, and 1.6m in height. The specific dimensions can be made according to the specific implementation.
[0057] Specifically, such as Figure 2 As shown, the two ends of the drive shaft 21 are symmetrically rotated and mounted on the water tank 1. The outer surface of the middle part of the drive shaft 21 is connected to the first drive belt 22. The first drive belt 22 is symmetrically fixedly mounted with a fixing plate 23. The fixing plate 23 is fixedly mounted with a folding plate 24. The end of the folding plate 24 away from the fixing plate 23 is fixedly mounted on the light source module 12. The end of the light source module 12 away from the folding plate 24 is mounted with a positioning device 25. The outer surface of the water tank 1 is mounted with a detection device 26 on the side close to the positioning device 25. The positioning device 25 and the detection device 26 are electrically connected.
[0058] The first transmission belt 22 is equipped with a damping device in its middle. Rotating the first transmission belt 22 allows for position switching of the light source module 12. Simultaneously, pushing the light source module 12 towards the middle of the transmission shaft 21 causes the folding plate 24 to fold, facilitating rapid position switching of the light source module 12. This design specifically considers the reliability of the equipment under special operating conditions. For example, if one side of the light source module 12 fails unexpectedly, the operator can continue working using the other side of the device or its backup state by rotating and adjusting the first transmission belt 22. This bidirectional operation / redundancy design effectively prevents unexpected interruptions to the experiment and ensures the continuity of the experimental process.
[0059] Specifically, as shown in Figure 2 The filter switching mechanism 3 includes an adjusting shaft 32 symmetrically arranged at both ends of the water tank 1, the outer surface of both ends of the adjusting shaft 32 is rotatably mounted on the water tank 1, both ends of the filter paper 31 are wound in the middle of the adjusting shaft 32, the outer surface of one end of the adjusting shaft 32 is fixedly mounted with a belt pulley 33, the second transmission belt 34 is drivingly mounted on the belt pulley 33, the damping wheel 35 is mounted on the side of the water tank 1 close to the belt pulley 33, the second transmission belt 34 is drivingly connected to the outer surface of the damping wheel 35 away from the adjusting shaft 32, the middle part of both ends of the water tank 1 is provided with light transmission glass, and the inner side of the filter paper 31 slides away from the middle part of the water tank 1.
[0060] Wherein, the light source module 12 is installed with light transmission glass at both ends, the adjusting shaft 32 can be rotated by rotating the handle at one end of the transmission shaft 21, the filter paper 31 can be wound by rotating the adjusting shaft 32, since the filter paper 31 is wound with different light transmission filter membranes in multiple sections on the adjusting shaft 32, the different light transmission filter membranes of the filter paper 31 can be switched by rotating the adjusting shaft 32, compared with the prior art of manually pasting filter paper on the lens, not only the operation is complicated, but also the used filter paper cannot be reused, resulting in waste of materials, through the design of the scheme, different light filtering requirements can be switched by rotating the transmission shaft 21, at the same time, the filter paper 31 can be wound by rotating the adjusting shaft 32 after use, not only can prevent dust from adhering to the surface of the filter paper 31, but also the scheme can be reused, and the operation is convenient, which is beneficial to the operation of the experimenters.
[0061] Specifically, as shown in Figure 2 The adjusting ring 41 is fixedly mounted with a clamping shaft 42 in the middle, the clamping shaft 42 is uniformly provided with a clamping groove, and the L-shaped pawl 43 is clamped in the clamping groove, the L-shaped pawl 43 is rotatably connected with a rotating shaft at the middle corner, and the rotating shaft is provided with a torsional spring, one end of the torsional spring is fixedly mounted on the water tank 1, the other end of the torsional spring is fixedly mounted on the L-shaped pawl 43, the clamping shaft 42 is fixedly connected with a gear 44 away from the adjusting ring 41, the gear 44 is symmetrically meshed with a sliding rack 45 on both sides of the gear 44, the sliding rack 45 is slidingly mounted on the water tank 1 on the upper surface, the sliding rack 45 is fixedly connected with a baffle 46 away from the gear 44, the baffle 46 is slidingly arranged in the water tank 1, the water tank 1 is slidingly connected with a guide block 47 on the inner wall, the guide block 47 is sleeved with a return spring 48 on the outer surface, one end of the return spring 48 is fixedly connected with the guide block 47, the other end of the return spring 48 is fixedly mounted on the water tank 1, the guide block 47 is fixedly connected with the baffle 46 away from the middle part of the water tank 1, and the contact point between the baffle 46 and the inner wall of the water tank 1 is provided with a waterproof sealing strip;
[0062] Wherein, the scheme is provided with inclined grooves at both ends of the bottom of the water tank 1, as shown in Figure 9As shown. By rotating the threaded adjusting rod 56, the rack 55 is driven, which in turn drives the rotating adjusting gear 54 to rotate. The rotation of the rotating adjusting gear 54 causes the adjusting plate 53 to adjust its angle. By changing the angle of the adjusting plate 53, the flow rate of the water can be controlled. This design can create different water flow environments inside the main body, more realistically simulating the natural water flow state, thereby improving the accuracy of the experiment and meeting the control requirements for flexible adjustment of different flow rates.
[0063] Specifically, such as Figure 2 As shown, the pressurizing device 51 is installed in the middle of the water tank 13. A nozzle 52 is installed on the pressurizing device 51. The nozzle 52 is fixedly installed on the water tank 13. The bottom right side of the water tank 13 is set as an inclined slope with a slope of 0-2 degrees. An adjusting plate 53 is evenly rotated and installed on the bottom slope of the water tank 13. An adjusting tooth 54 is fixedly installed on the outer surface of one end of the adjusting plate 53. A toothed rack 55 is engaged with the tooth surface of the adjusting tooth 54. The toothed rack 55 is slidably installed on the bottom slope of the water tank 13. A threaded adjusting rod 56 is rotatably installed on the end of the toothed rack 55 away from the pressurizing device 51. The threaded adjusting rod 56 is threadedly installed on the inner wall of the water tank 1 at the end away from the toothed rack 55. A waterproof sealing gasket is installed on the outside of the threaded connection between the toothed rack 55 and the threaded adjusting rod 56 for sealing and waterproofing inside the water tank 1.
[0064] In this design, inclined slots are provided at both ends of the bottom of the water tank 1. By rotating the adjusting screw 56, the rack 55 can be driven to rotate the adjusting gear 54. After the adjusting gear 54 rotates, the adjusting plate 53 can be adjusted in angle. The angle adjustment of the adjusting plate 53 can control the flow speed of the water, thereby creating different water flow environments in the water tank 1 to better simulate the water flow conditions in the real environment. This is beneficial to the accuracy of the experiment and also satisfies the adjustable controllability of different water flows.
[0065] Example 2, a method for screening adult fish for sensitive light environments, includes the following steps:
[0066] Step 1: Adjust the filter paper at both ends of the water tank. Switch the filter paper by rotating the middle of one end of the adjustment shaft to create a light environment for the specific spectral color to be tested.
[0067] Step 2: Turn on the light source module and use an illuminance meter to measure the light environment of the water tank to obtain its distribution range; use a dark environment as a control group.
[0068] Step 3: Fine-tune the adjustment mechanism according to the size of the fish to make the size of the tank more suitable for the movement of the experimental fish. Put the experimental fish into the tank and adjust the water flow control component to make it a still water environment. After the fish adapts, turn on the video monitoring system to observe and record the behavior of the experimental fish swimming freely in the tank.
[0069] Step four, set up a control experiment group, and carry out a control experiment on fish in a dark environment, so as to exclude system errors of the experiment;
[0070] Step five, carry out a to-be-tested light environment experiment on fish: in the same time period as the free-swimming behavior experiment, carry out a to-be-tested light environment experiment on fish, filter light according to switching different filter papers, set the behavior and swimming speed of fish under different light environment stimulation, analyze and determine the properties of phototaxis of fish, and thus screen out positive phototaxis and negative phototaxis respectively.
[0071] When screening positive phototaxis, the light source module 12 located on both sides of the water tank 1 in the scheme is the first light source device and the second light source device respectively, which is conducive to distinguishing the test, the second light source device far from the experimental fish at one end of the water tank 1 is turned on, when the experimental fish swims towards the light source and passes through the middle of the water tank 1, the light intensity of the second light source device is reduced to 0 lx, and the first light source device at the other end of the water tank 1 is turned on; when the experimental fish shows positive phototaxis reaction to the to-be-tested light environment, the fish will turn around and swim towards the first light source device, when the experimental fish swims through the middle of the water tank 1 again and swims towards the second light source device, the first light source device is turned off, and the second light source device is turned on again, and the cycle is repeated for 20 minutes or until the experimental fish no longer reacts; the swimming behavior of fish is recorded by the video monitoring system, the average speed of fish swimming towards the light source, the average speed of turning around, and the number of turning around are calculated, if the average speed of fish swimming towards the light source, the average speed of turning around, and the number of turning around are higher than the corresponding average speed and turning around number when the fish freely swims, then the screened out is positive phototaxis; when screening positive phototaxis, the specific method for calculating the average speed of fish swimming and the average speed of turning around is as follows: when the fish freely swims, the time from starting to swim to reaching the middle of the water tank 1 is t1, and the distance is d1, then the average speed is d1 / t1, and the time from 1m before turning around to 1m after turning around is t2, then the average speed of turning around is 2 / t2; when the light source device is started, the time from starting to react to reaching the middle of the water tank 1 is T1, the distance is D1, then the average speed of swimming towards the light source is D1 / T1, and the time from 1m before turning around to 1m after turning around is T2, then the average speed of turning around is 2 / T2;
[0072] When screening for negative phototaxis, the first light source device near one end of the water tank 1 is turned on, and when the experimental fish swims away from the light source and passes through the middle of the water tank 1, the light intensity of the first light source device is reduced to 0 lx, and the second first light source device at the other end of the water tank 1 is turned on. When the experimental fish exhibits negative phototaxis to this test light environment, the fish will turn around and swim towards the first light source device. When the experimental fish swims through the middle of the water tank 1 again towards the first light source device, the second first light source device is turned off and the first light source device is turned on again. This cycle is repeated for 20 minutes or until the experimental fish no longer responds. The swimming behavior of the fish is recorded by the video monitoring system, and the average speed of the fish swimming away from the light source, the average speed of turning around, and the number of turns are calculated. If the average speed of the fish swimming away from the light source, the average speed of turning around, and the number of turns are higher than the corresponding average speed and the number of turns when the fish swims freely, then the screened is negative phototaxis. When screening for negative phototaxis, the specific method for calculating the average speed of the fish swimming away from the light source and the average speed of turning around is as follows:
[0073] When the fish swims freely, the time from starting to swim to reaching the middle of the water tank 1 is t1, and the distance is d1, then the average speed is d1 / t1, and the time from 1m before turning around to 1m after turning around is t2, then the average speed of turning around is 2 / t2. When the light source device is turned on, let the time from starting to respond to reaching the middle of the water tank 1 be T3, and the distance traveled be D3, then the average speed of swimming away from the light source is D3 / T3, and the time from 1m before turning around to 1m after turning around is T4, then the average speed of turning around is 2 / T4.
[0074] As shown in Figure 1 The device for screening the light-sensitive environment of adult fish includes a water tank 1, a first light source device at one end of the water tank 1, and a second first light source device at the other end of the water tank 1. The water tank 1 is also provided with a video monitoring system for monitoring the swimming behavior of the fish and a support for adjusting the monitoring height. Light source devices are placed at both ends of the water tank 1, and the light sources are turned on alternately. The experimental fish will exhibit a clear "turning" behavior when swimming towards or away from the light source, allowing accurate and convenient observation and judgment of the phototaxis and sensitivity of adult fish.
[0075] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting; it is not intended to exclude myriad other embodiments of the present application that other inventors can develop based on the same general inventive concepts embodied by the described embodiments. That is, although the present application is described in terms of particular embodiments and implementations, it is to be understood that the terminology used is for the purpose of descriptive clarity and that it is intended to be limited only by the words recited in the appended claims. The scope of the present application shall be limited only by the claims.
[0076] While the embodiments of the application have been shown and described herein, it is to be understood that the application is not limited to these embodiments. Rather, numerous modifications are possible without departing from the spirit and scope of the present application as delineated by the claims and their equivalents.
Claims
1. A method for screening sensitive light sources for adult fish, characterized in that, include: S1, adjust the filter paper (31) at both ends of the water tank (1), and switch the filter paper (31) by rotating the middle of one end of the adjustment shaft (32) to create a light environment for the specific spectral color to be tested; S2, turn on the light source module (12), and at the same time use an illuminance meter to measure the light environment of the water tank (1) to obtain its distribution range; and use the dark environment as the control group; S3. According to the size of the fish, the adjustment mechanism (4) is finely adjusted so that the size of the tank (1) is more suitable for the movement of the experimental fish. The experimental fish is placed in the tank (1), and the water flow control component (5) is adjusted to make it a still water environment. After the fish adapts, the video monitoring system is turned on to observe and record the behavior of the experimental fish swimming freely in the tank (1). S4. Set up a control experimental group to conduct a control experiment on fish (in a dark environment) to eliminate systematic errors in the experiment; S5, Conduct a test light environment experiment on fish: During the same time period as the free swimming behavior experiment, conduct a test light environment experiment on fish, filter light by switching different filter paper (31), set the swimming behavior and swimming speed of fish under different light environment stimuli, analyze and judge the phototaxis of fish, and thus screen out positive phototaxis and negative phototaxis respectively.
2. The method for screening sensitive light sources for adult fish according to claim 1, characterized in that, In S4, when the environment is dark, the light source modules (12) at both ends of the water tank (1) are set as the first light source and the second light source, respectively. When the first light source is turned on at the end of the water tank (1) near the experimental fish, when the experimental fish swims away from the light source and passes through the middle of the water tank (1), the light intensity of the first light source is reduced to 0 lx, and the second light source at the other end of the water tank is turned on. When the experimental fish swims through the middle of the water tank (1) again and swims towards the first light source device, the second light source device is turned off and the first light source device is turned on. This cycle is repeated for 20 minutes or until the experimental fish no longer reacts.
3. The method for screening sensitive light sources for adult fish according to claim 1, characterized in that, In step S5, when the fish being screened are phototactic, the first light source device at the end of the tank furthest from the experimental fish is turned on. When the experimental fish swims towards the light source and passes through the middle of the tank, the light intensity of the first light source device is reduced to 0 lx, and at the same time, the second light source device at the other end of the tank is turned on. When the experimental fish exhibits a positive phototactic response to this light environment, the fish will turn around and swim towards the second light source device. When the experimental fish swims through the middle of the tank and towards the first light source device again, the second light source device is turned off, and the first light source device is turned on again. This cycle is repeated for 20 minutes or until the experimental fish no longer react.
4. A device for screening sensitive light sources for adult fish, applicable to the method for screening sensitive light sources for adult fish as described in any one of claims 3 (1), comprising a water tank (1), the water tank (1) being symmetrically arranged, a water flow channel (13) being installed at the bottom of the water tank (1), a support column (11) being installed at the bottom of the water flow channel (13), and light source modules (12) being provided at both ends of the water tank (1), characterized in that, It also includes a quick-switching monitoring mechanism (2), a filter switching mechanism (3), and an adjustment mechanism (4); The quick-switching monitoring mechanism (2) is set in the middle of the water tank (1) symmetrically arranged, and the quick-switching monitoring mechanism (2) is used to adjust the switching of the light source module (12); The filter switching mechanism (3) is set on the outer surface of the water tank (1) and includes filter paper (31). The filter paper (31) is set on the outer surfaces of both ends of the water tank (1). The filter switching mechanism (3) is used to adjust the filter paper (31). The adjustment mechanism (4) is installed in the water tank (1) and is used to adjust the internal volume of the water tank (1) and control the water flow.
5. The device for screening adult fish sensitive light sources according to claim 4, characterized in that: The quick-switching monitoring mechanism (2) includes a drive shaft (21), which is symmetrically mounted on the water tank (1) at both ends. A first drive belt (22) is connected to the outer surface of the middle part of the drive shaft (21). A fixing plate (23) is symmetrically fixed on the first drive belt (22). A folding plate (24) is fixedly mounted on the fixing plate (23). The end of the folding plate (24) away from the fixing plate (23) is fixedly mounted on the light source module (12). A positioning device (25) is installed on the end of the light source module (12) away from the folding plate (24). A detection device (26) is installed on the outer surface of the water tank (1) near the positioning device (25). The positioning device (25) and the detection device (26) are electrically connected.
6. The device for screening adult fish sensitive light sources according to claim 4, characterized in that: The filter switching mechanism (3) includes an adjustment shaft (32), which is symmetrically arranged at both ends of the water tank (1). The outer surfaces of both ends of the adjustment shaft (32) are rotatably mounted on the water tank (1). The two ends of the filter paper (31) are wound around the middle of the adjustment shaft (32). A pulley (33) is fixedly mounted on the outer surface of one end of the adjustment shaft (32). A second transmission belt (34) is driven on the pulley (33). A damping wheel (35) is installed on the side of the water tank (1) near the pulley (33). The side of the second transmission belt (34) away from the adjustment shaft (32) is driven to the outer surface of the damping wheel (35). The middle of both ends of the water tank (1) is set as a light-transmitting glass, and the inner side of the filter paper (31) slides on the side of the light-transmitting glass away from the middle of the water tank (1).
7. The device for screening adult fish sensitive light sources according to claim 4, characterized in that: The adjusting mechanism (4) includes an adjusting ring (41), a retaining shaft (42) is fixedly installed in the middle of the adjusting ring (41), the retaining shaft (42) is evenly provided with retaining grooves, and an L-shaped pawl (43) is engaged in the retaining grooves. A rotating shaft is rotatably connected at the corner of the L-shaped pawl (43), and a torsion spring is installed on the rotating shaft. One end of the torsion spring is fixedly installed on the water tank (1), and the other end of the torsion spring is fixedly installed on the L-shaped pawl (43). A gear (44) is fixedly connected to the end of the retaining shaft (42) away from the adjusting ring (41). A sliding rack (45) is symmetrically meshed on both sides of the gear (44). The upper surface of the sliding rack (45) is slidably mounted on the gear. On the water tank (1), a baffle (46) is fixedly connected to the end of the sliding rack (45) away from the gear (44). The bottom of the baffle (46) slides in the water tank (1). Guide blocks (47) are slidably connected to the inner walls on both sides of the water tank (1). A reset spring (48) is sleeved on the outer surface of the guide block (47). One end of the reset spring (48) is fixedly connected to the guide block (47), and the other end of the reset spring (48) is fixedly installed on the water tank (1). The end of the guide block (47) near the middle of the water tank (1) is fixedly connected to the baffle (46). A waterproof sealing strip is provided at the contact point between the baffle (46) and the inner wall of the water tank (1).
8. The device for screening adult fish sensitive light sources according to claim 5, characterized in that: The adjustment mechanism (4) further includes a pressurizing device (51), which is installed in the middle of the water trough (13). A nozzle (52) is installed on the pressurizing device (51), and the nozzle (52) is fixedly installed on the water trough (13). The bottom right side of the water trough (13) is set as an inclined slope with a slope of 0-2 degrees. An adjustment plate (53) is evenly rotated and installed on the bottom slope of the water trough (13). An adjustment tooth (5) is fixedly installed on the outer surface of one end of the adjustment plate (53). 4) The tooth surface of the adjusting tooth (54) is engaged with a toothed rack (55). The toothed rack (55) is slidably installed on the bottom slope of the water tank (13). A threaded adjusting rod (56) is rotatably installed on the end of the toothed rack (55) away from the pressurizing device (51). The threaded adjusting rod (56) is threadedly installed on the inner wall of the water tank (1) at the end away from the toothed rack (55). A waterproof sealing gasket is installed on the outside of the threaded connection between the toothed rack (55) and the threaded adjusting rod (56) for sealing and waterproofing inside the water tank (1).