Biological feed containing degreased ganoderma lucidum spore powder and compound bacteria and application of biological feed

Abstract

The invention belongs to the technical field of biology, and discloses a feed additive which comprises degreased ganoderma lucidum spore powder, clostridium butyricum and bacillus coagulans. The additive provides the following components for each kilogram of basal feed: 50-200mg of degreased ganoderma lucidum spore powder; clostridium butyricum is 1 * 10 < 8 > cfu to 10 * 10 < 8 > cfu; and bacillus coagulans is 1 * 10 < 9 >-10 * 10 < 9 > cfu. Experimental verification shows that the combination of clostridium butyricum and bacillus coagulans and the degreased ganoderma lucidum spore powder can significantly improve and improve the intestinal health and production performance of piglets. Meanwhile, the invention further provides application of the additive and a biological feed containing the degreased ganoderma lucidum spore powder and the compound bacteria.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biotechnology, in particular to a feed additive, a biological feed containing defatted ganoderma lucidum spore powder and compound bacteria and application thereof. BACKGROUND

[0002] The application of antibiotics in feed is a "double-edged sword", which can prevent diseases and promote animal growth, but due to drug resistance and other problems, it seriously threatens the quality of livestock and poultry products and public health safety and is criticized by society.

[0003] Defatted ganoderma lucidum spore powder is a large amount of by-products produced after the extraction of spore oil from ganoderma lucidum spore powder. Usually, a small part of these by-products is used as fertilizer or feed, and most of them are discarded without treatment, which not only pollutes the environment but also causes great waste of resources.

[0004] In the prior art, the application of defatted ganoderma lucidum spore powder is mostly for extracting polysaccharides or as a food additive, such as the extraction method of ganoderma lucidum spore polysaccharide, its product and application with the publication number CN120157777A.

[0005] It is undeniable that the use of ganoderma lucidum spore powder in breeding is universal. However, due to the broken wall and defatting, the usability of defatted ganoderma lucidum spore powder is greatly reduced.

[0006] Therefore, the technical problem to be solved by the present application is how to economically utilize defatted ganoderma lucidum spore powder. SUMMARY

[0007] The purpose of the present application is to provide a feed additive. Through screening, we found that the combination of clostridium butyricum, bacillus coagulans and defatted ganoderma lucidum spore powder can significantly improve and improve the intestinal health and production performance of piglets.

[0008] Meanwhile, the application of the additive is also provided.

[0009] To achieve the above purpose, the present application provides the following technical scheme: a feed additive, the additive comprising defatted ganoderma lucidum spore powder, clostridium butyricum and bacillus coagulans;

[0010] The additive provides the following components per kilogram of basic feed:

[0011] Defatted ganoderma lucidum spore powder 50-200mg;

[0012] Clostridium butyricum 1x10 8 cfu~10x10 8 cfu;

[0013] Bacillus coagulans 1x10 9 ~10x109 cfu.

[0014] In the above-mentioned feed additive, Bacillus licheniformis is further included, 1x10 9 ~ 10x10 9 cfu Bacillus licheniformis is added per kilogram of base feed.

[0015] In the above-mentioned feed additive, the additive provides the following components per kilogram of base feed:

[0016] Ganoderma lucidum spore powder 50-200 mg;

[0017] Clostridium butyricum 1x10 8 cfu ~ 10x10 8 cfu;

[0018] Bacillus licheniformis 1x10 9 ~ 5x10 9 cfu;

[0019] Bacillus coagulans 5x10 9 ~ 10x10 9 cfu.

[0020] Meanwhile, the application further discloses a biological feed, comprising base feed and premix; the premix comprises the feed additive as mentioned above.

[0021] In the above-mentioned biological feed, the premix provides the following components per kilogram of base feed:

[0022] Cu 90-120 mg;

[0023] Zn 80-100 mg;

[0024] Fe 110-150 mg;

[0025] Mn 70-90 mg;

[0026] Se 0.25-0.35 mg;

[0027] I 0.1-0.2 mg.

[0028] In the above-mentioned biological feed, the premix provides the following components per kilogram of base feed:

[0029] VA 1800-2200 IU;

[0030] VD3 1350-1650 IU;

[0031] VE 48-58 mg;

[0032] VK3 0.9-1.1mg;

[0033] VB1 5.5-6.5mg;

[0034] VB2 2.5-3.1mg;

[0035] VB6 2.5-3.1mg;

[0036] VB 12 0.09-0.11mg;

[0037] Folic acid 1.8-2.2mg;

[0038] Niacin 7-9mg;

[0039] Pantothenic acid 25-31mg;

[0040] Biotin 0.18-0.22mg.

[0041] In the above-mentioned biological feed, the premix provides the following components per kilogram of basal feed:

[0042] Phytase 450-550U / kg;

[0043] Acid protease 110-130U / kg;

[0044] Beta-glucanase 45-55U / kg;

[0045] Xylanase 140-16U / kg.

[0046] In the above-mentioned biological feed, the basal feed is a pig feed, a chicken feed or a duck feed.

[0047] Finally, the present application also provides the use of the feed additive as described above for preparing a feed.

[0048] In the above-mentioned use, the feed is a pig feed, a chicken feed or a duck feed

[0049] Compared with the prior art, the present application has the following beneficial effects:

[0050] The present application improves the average daily weight gain of pigs, reduces the feed-meat ratio, increases short-chain fatty acids such as butyric acid and propionic acid in the intestine, promotes the proliferation of probiotics such as lactic acid bacteria and bifidobacteria in the intestine, inhibits the growth of pathogenic bacteria such as Escherichia coli, and protects the intestinal microecological balance.

[0051] By adding defatted ganoderma lucidum spore powder and butyric acid clostridium and other probiotics in feed, the secretion of pig immunoglobulin (IgA, IgG) is promoted, the immune function is enhanced, the release of pro-inflammatory factors (such as IL-6, TNF-α) is inhibited, the oxidation stress of the body is relieved, the disease resistance is improved, and the dead and discarded rate is reduced.

[0052] The above experiment proves that the additive of the present application has good intestinal health improvement effect, and is a feed additive which can realize full utilization of agricultural waste and has high breeding utilization value. BRIEF DESCRIPTION OF DRAWINGS

[0053] Figure 1 is a column chart of average daily weight gain of pigs at different growth stages in experiment one;

[0054] Figure 2 is the intestinal microbial detection result of piglets in experiment one;

[0055] Figure 3 is a column chart of the content of acetic acid, propionic acid, butyric acid, isobutyric acid, iso-valeric acid and valeric acid at different growth stages in experiment one;

[0056] Figure 4 is a diagram of intestinal tissue morphology of piglets in experiment one. DETAILED DESCRIPTION

[0057] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0058] Raw material sources:

[0059] Clostridium butyricum group and Bacillus coagulans: provided by Zhejiang Qirun Biological Technology Co., Ltd.;

[0060] Bacillus licheniformis: SNKM-21 24419 Bacillus licheniformis preserved by Shanghai Academy of Agricultural Sciences, preservation number: CGMCC No.24419; preservation date: February 22, 2022, preservation unit: China General Microbiological Culture Collection Center.

[0061] Defatted ganoderma lucidum spore powder: defatted ganoderma lucidum spore powder is provided by Shanghai Bai Xin Biological Technology Co., Ltd., which is a processing by-product after the spore oil of ganoderma lucidum spore powder is broken and extracted by supercritical carbon dioxide, and the content of ganoderma triterpenes is 1.3%, and the content of polysaccharides is 1.5%.

[0062] Experiment 1: Regulation mechanism of Clostridium butyricum on intestinal health of piglets

[0063] To study the effects of Clostridium butyricum on the production performance, nutrient digestion and utilization and intestinal health of piglets, and to compare with the effects of conventional feed antibiotics and sodium butyrate, the feasibility of Clostridium butyricum replacing antibiotics in feed was explored, and the regulation mechanism of Clostridium butyricum on intestinal health of piglets was revealed, which provided experimental basis for the development of compound probiotics and the compatibility with defatted ganoderma lucidum spore powder in the later stage.

[0064] 1. Materials and methods

[0065] 1.1 Experimental animals and management

[0066] In this experiment, 80 weaned DLY (Duroc × Landrace × Yorkshire) piglets of 28 ± 1 days old were randomly divided into basal diet group (without antibiotics, formula see Table 1), antibiotic group (40 g / kg colistin sulfate + 20 mg / kg aureomycin), Clostridium butyricum group (5 × 10 8 CFU / kg) and sodium butyrate group (500 mg / kg), with 4 replicates in each group, 5 pigs in each replicate, and a total of 20 pigs. The experimental animals were purchased from Shanghai Pig Farm, and the piglets in each group were fed with the corresponding experimental diet, free to eat and drink, and the feeding management was carried out according to the conventional feeding management mode.

[0067] The experiment lasted for 5 weeks, and the feed intake of the experimental pigs was recorded every day. The pigs' feces, urine, feeding habits and health status were observed and recorded in time. The weight of each group of piglets was recorded every week, and the feed intake (FI), average daily gain (ADG) and feed conversion efficiency (F / G) were calculated.

[0068] Table 1. Formula of basal diet

[0069]

[0070]

[0071] Note: The premix provides per kg of feed: Cu ≥ 100 mg, Fe ≥ 120 mg, Zn ≥ 75 mg, Mn ≥ 50 mg, Se ≥ 0.3 mg, I ≥ 0.14 mg, VA 2000 IU, VD3 1500 IU, VE 53 mg, VK3 1 mg, VB1 6 mg, VB2 2.8 mg, VB6 2.8 mg, VB 12 0.1 mg, folic acid 2 mg, niacin 8 mg, pantothenic acid 28 mg, biotin 0.2 mg.

[0072] 1.2 Nutrient apparent digestibility

[0073] At 2 and 4 weeks of the experiment, fecal samples were collected using stainless steel metabolic cages, mixed and then sampled. 10% hydrochloric acid was added to the fecal samples according to the weight of the fecal samples. The dry matter and crude protein contents of the feed and fecal samples were determined, and the apparent digestibility of dry matter and crude protein in the piglets was calculated using acid-insoluble ash as an endogenous indicator.

[0074] 1.3 Intestinal microorganism detection

[0075] Fecal samples were collected aseptically from piglets using rectal stimulation. LB medium, MRS medium plate counting and lactose fermentation tube test were used to detect the number of total bacteria, lactic acid bacteria and E. coli in the fecal samples. Fecal microbial DNA was extracted using a fecal sample DNA extraction kit (Solarbio Company) and stored at -20°C. The DNA concentration was determined using a nucleic acid concentration detector at 260 nm and 280 nm, and the DNA concentration was adjusted to 20 ng / μL using DEPC-treated double distilled water. Based on the 16S rRNA gene sequence of bacteria, real-time fluorescent quantitative PCR was used to detect the number of total bacteria, lactic acid bacteria, enterobacteria and Clostridium butyricum using specific primers. The primers were synthesized by GenScript Biotech (Shanghai) Co., Ltd., and the primer sequences and related information are shown in Table 2. The number of changes in total bacteria, enterobacteria, lactic acid bacteria and Clostridium butyricum in feces was determined using an absolute quantitative method using a real-time fluorescent quantitative PCR instrument (Applied Biosystems 7500). The real-time fluorescent quantitative PCR reaction conditions were as follows: 95°C for 5 min; 95°C for 15 s, 60°C for 1 min, 6 cycles; 95°C for 15 s, 58-64°C for 1 min, 80°C for 30 s, and the fluorescence value was read at 80°C, 40 cycles; the reaction system was 20 μL (see Table 3).

[0076] Table 2. Real-time fluorescent quantitative PCR primer information

[0077]

[0078] Table 3. Real-time fluorescent quantitative PCR reaction system composition

[0079]

[0080]

[0081] 1.4 Short-chain fatty acid content determination

[0082] Accurately take 1 g of fecal sample into a 10 ml centrifuge tube, add 5 mL of double distilled water, mix well, take 1 mL of supernatant, add 0.2 mL of metaboric acid, and store at -20°C overnight. After thawing, centrifuge at 12000 rpm for 10 min, and take the supernatant and store at -20°C. Use metaboric acid as an internal standard to detect the concentrations of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, and hexanoic acid in the sample solution by gas chromatography, and calculate the content of short-chain fatty acids in the fecal sample based on the mass of the fecal sample.

[0083] 1.5 Organ index determination

[0084] At the end of the 5th week of the experiment, the piglets were weighed on an empty stomach, blood was collected, and the piglets were put to sleep with carbon dioxide, and blood was collected after the piglets were put to sleep. The weights of the heart, liver, spleen, kidney, stomach, duodenum, jejunum, ileum, colon, and cecum were measured, and the ratios of the weights of the organs and intestinal segments to the body weight were calculated.

[0085] 1.6 Intestinal tissue morphological analysis

[0086] Immediately after the piglets were slaughtered, jejunum tissue was collected, washed with physiological saline, fixed with formaldehyde, and paraffin-embedded for sectioning. The paraffin sections of the intestinal tissue were stained with hematoxylin-eosin, and the morphological structure integrity of the intestinal tissue was evaluated under a microscope. The villus height and crypt depth were measured, and the ratio of the villus height to the crypt depth was calculated.

[0087] 2. Results and analysis

[0088] 2.1 Production performance

[0089] The production performance of the piglets in each group during the entire experiment is shown in Table 4. There was no significant difference in feed intake among the groups. The piglets in the Clostridium butyricum group had the highest weight gain and the lowest feed conversion ratio, followed by the piglets in the antibiotic group, and the piglets in the sodium butyrate group had the worst performance, but the differences among the groups were not significant (p>0.05). The average daily gain (ADG) of the piglets in the Clostridium butyricum group was significantly higher than that of the piglets in the antibiotic group (p=0.043) in the first week of the experiment, and was extremely significantly higher than that of the piglets in the basal diet group (p=0.006) in the fourth week of the experiment. The differences in weight gain among the groups in the other stages were not significant (p>0.05). Figure 1

[0090] Table 4. Production performance of piglets during the entire experiment

[0091] Group Control group Antibiotic group Clostridium butyricum group Sodium butyrate group Initial weight (kg) 8.58±0.48 8.88±0.49 8.75±0.44 8.73±0.43 Final weight (kg) 17.89±1.02 18.23±1.59 19.51±0.99 17.44±1.11 Total weight gain (kg) 9.04±0.57 9.30±1.06 10.30±0.77 8.68±0.72 Feed conversion ratio 1.73±0.05 1.68±0.03 1.49±0.04 1.75±0.03

[0092] 2.2 Organ index

[0093] ​The results of organ index analysis of piglets (Table 5) showed that the spleen index of piglets in the antibiotic group was the highest, higher than that in the sodium butyrate group (p<0.05) and the basal diet group (p=0.053); the ileum index of piglets in the antibiotic group was the lowest, significantly lower than that in the Clostridium butyricum group and the sodium butyrate group (p<0.05); the colon index of piglets in the sodium butyrate group was the highest, increased by 11.66%, 9.10% and 9.44% compared with the basal diet group, the antibiotic group and the Clostridium butyricum group, respectively, but the difference did not reach a significant level (p>0.05).

[0094] Table 5. Organ index of piglets

[0095]

[0096]

[0097] 2.3 Digestibility of nutrients

[0098] As shown in Table 6, there was no significant difference in the apparent digestibility of dry matter and crude protein of piglets in each group at the 2nd and 4th weeks. The apparent digestibility of crude protein of piglets in the sodium butyrate group was the lowest at each stage, which was 7.18% lower than that in the control group at the 2nd week, but the difference did not reach a significant level (p>0.05).

[0099] Table 6. Apparent digestibility of nutrients

[0100]

[0101] 2.4 Intestinal microorganisms

[0102] The results of microbial count of fecal samples showed that the number of Escherichia coli in the feces of piglets in the antibiotic group and the Clostridium butyricum group was significantly lower than that in the control group (p<0.05) at the 2nd week, and the number of lactic acid bacteria in the feces of piglets in the Clostridium butyricum group was extremely significantly higher than that in the control group (p<0.01). At the 4th week, the total number of bacteria in the feces of piglets in the antibiotic group was significantly lower than that in the control group (p<0.01), and the number of Escherichia coli in the feces of piglets in the Clostridium butyricum group was significantly lower than that in the control group (p<0.05). Figure 2 The results of quantitative PCR detection of microorganisms showed that the addition of antibiotics tended to reduce the total number of bacteria in the feces of piglets at the 1st week, but significantly increased the number of enterobacteria (p<0.01); the addition of Clostridium butyricum in feed had no significant effect on the total number of bacteria and the number of enterobacteria in the feces, but significantly increased the number of lactic acid bacteria and Clostridium butyricum (p<0.01); sodium butyrate had no significant effect on the total number of bacteria in the feces, but significantly increased the number of enterobacteria and lactic acid bacteria (p<0.01). The number of main microorganisms in the feces of piglets at other stages showed similar regularity.

[0103] 2.5 Short-chain fatty acid content

[0104] As shown in Table 7, the content of short-chain fatty acids in the feces of piglets in the antibiotic group was the highest, which was 1.48 times and 1.27 times higher than that in the control group and the Clostridium butyricum group, respectively, and the difference reached a significant level (p<0.05); the content of short-chain fatty acids in the feces of piglets in the sodium butyrate group was the lowest, which was 0.62 times and 0.74 times lower than that in the control group and the Clostridium butyricum group, respectively, and the difference reached a significant level (p<0.05). Figure 3As shown, the addition of Clostridium butyricum and sodium butyrate to the diet significantly increased the levels of acetic acid, propionic acid, butyric acid, isobutyric acid, isovaleric acid, and valeric acid in the rectal contents of piglets during the first week. The SCFAs with the largest increases were acetic acid, propionic acid, and butyric acid. In the second week of the experiment, Clostridium butyricum significantly increased the propionic acid content in feces (p<0.01), while sodium butyrate had no significant effect on the propionic acid content in piglet feces (p>0.05). Neither Clostridium butyricum nor sodium butyrate had a significant effect on the isovaleric acid content in the piglet intestine (p>0.05), while their effects on the levels of acetic acid, isobutyric acid, and butyric acid showed a similar trend to the first week.

[0105] 2.6 Intestinal tissue morphology and structure

[0106] Depend on Figure 4 It was found that the jejunal villi in the control group were more disordered, while the villi in the other three groups were more densely and orderly arranged. The villi in the Clostridium butyricum group and the sodium butyrate group were longer and thinner than those in the antibiotic group. The jejunal villi height and the villi height / crypt depth ratio in the Clostridium butyricum group and the sodium butyrate group were significantly higher than those in the control group and the antibiotic group, while the crypt depth in the Clostridium butyricum group was significantly lower than that in the control group.

[0107] 3. Conclusion

[0108] During weaning, the drastic changes in the source, composition, and digestibility of food disrupt the gut microbiota balance of piglets, damaging the integrity of the intestinal mucosa and leading to diarrhea and decreased production performance. Therefore, maintaining a stable gut microbiota structure and intestinal mucosal integrity in weaned piglets is crucial for ensuring gut health and preventing diarrhea.

[0109] This embodiment confirms that the application of *Clostridium butyricum* in the diet of weaned piglets to replace antibiotics not only does not lead to a decline in production performance, but the piglets in the *Clostridium butyricum* group showed the greatest weight gain and the highest feed conversion efficiency throughout the trial. *Clostridium butyricum* promotes the proliferation of beneficial bacteria such as lactic acid bacteria while inhibiting the growth of *Escherichia coli*, which is beneficial to the balance of the piglet's intestinal microecology and maintaining the integrity of the intestinal morphology and structure, thus promoting intestinal health. Therefore, adding *Clostridium butyricum* to a low-protein, amino acid-balanced diet for piglets to replace antibiotics is feasible.

[0110] Experiment 2: Effects of adding defatted Ganoderma lucidum spore powder and probiotics to feed on the production performance of piglets

[0111] This study investigates the effects of adding defatted Ganoderma lucidum spore powder and compound probiotics to feed on the production performance of piglets, using production performance as the main indicator, and explores the combined effect of the two, providing experimental basis and reference for the development of new biological feed formulations and products.

[0112] 1. Materials and Methods

[0113] 1.1 Laboratory Animals and Their Management

[0114] The experiment selected 210 weaned DLY (Duroc x Landrace x Yorkshire) piglets of 21±1 days of age, which were randomly divided into 4 groups according to the principle of uniform weight, and were fed with basal diet (group A), basal diet + defatted Ganoderma lucidum spore powder (group B, 100 g / t), basal diet + probiotics (group C, 5 x 10 8 CFU / kg butyric acid Clostridium group + 6 x 10 9 CFU / kg Bacillus coagulans + 4 x 10 9 CFU / kg Bacillus licheniformis), basal diet + defatted Ganoderma lucidum spore powder + probiotics (group D, 100 g / t defatted Ganoderma lucidum spore powder + 5 x 10 8 CFU / kg butyric acid Clostridium group), basal diet + defatted Ganoderma lucidum spore powder + probiotics (group E, 100 g / t defatted Ganoderma lucidum spore powder + 5 x 10 8 CFU / kg butyric acid Clostridium group + 10 x 10 9 CFU / kg Bacillus coagulans), basal diet + defatted Ganoderma lucidum spore powder + probiotics (group F, 100 g / t defatted Ganoderma lucidum spore powder + 5 x 10 8 CFU / kg butyric acid Clostridium group + 10 x 10 9 CFU / kg Bacillus licheniformis), basal diet + defatted Ganoderma lucidum spore powder + probiotics (group G, 100 g / t defatted Ganoderma lucidum spore powder + 5 x 10 8 CFU / kg butyric acid Clostridium group + 6 x 10 9 CFU / kg Bacillus coagulans + 4 x 10 9 CFU / kg Bacillus licheniformis). Each group had 6 replicates, 5 pigs per replicate, a total of 30 pigs. The defatted Ganoderma lucidum spore powder was provided by Shanghai Bai Xin Biological Technology Co., Ltd., which was a by-product of processing after the spore oil was extracted from the broken wall of Ganoderma lucidum spore powder by carbon dioxide supercritical extraction. The test detected that the content of ganoderma triterpenes was 1.3%, and the content of polysaccharides was 1.5%. The piglets in each group were fed with different feeds, and other feeding and management conditions were completely consistent. They were free to eat and drink, and the feeding and management was carried out according to the conventional feeding and management mode.

[0115] 1.2 Test animals and management

[0116] The test period was 4 weeks, and the feed intake of the test pigs was recorded every day. The pigs' feces, urine, feeding habits, and health status were observed and recorded in time. Each week, the weight of each group of piglets was measured, and the feed intake (FI), average daily gain (ADG), and feed conversion efficiency (F / G) were calculated. The diarrhea rate and culling rate were recorded and calculated every day.

[0117] 2. Results and analysis

[0118] The production performance of each group of piglets during the whole experiment is shown in Table 11. The experimental results show that, compared with the control group, the addition of defatted ganoderma lucidum spore powder and compound probiotics in feed can significantly improve the weight gain and feed intake of piglets, and reduce the feed conversion ratio. There is no significant difference in the production performance of piglets between the defatted ganoderma lucidum spore powder group and the compound probiotics group, but the production performance of piglets with the simultaneous addition of defatted ganoderma lucidum spore powder and compound probiotics is significantly better than that of piglets with the addition of a single test group. Only one piglet in the control group died during the experiment, and the compound probiotics significantly reduced the incidence of diarrhea in piglets. The above results show that the two functional feed additives have different functions and action pathways for improving the production performance of piglets, have obvious additive effects, and can complement each other to obtain better production performance when combined.

[0119] Table 11. Production performance and diarrhea of piglets

[0120]

[0121]

[0122] Conclusion:

[0123] 1. As shown by the above A group to C group, the performance of using compound probiotics alone is better than that of using defatted ganoderma lucidum spore powder alone;

[0124] 2. As shown by the D group to F group, the addition of clostridium butyricum and defatted ganoderma lucidum spore powder in the diet can significantly improve the diarrhea rate and reduce the feed conversion ratio; the use of bacillus coagulans can further improve the situation; the use of bacillus licheniformis is not as good as bacillus coagulans;

[0125] 3. As shown by the G group, the use of three kinds of bacteria can achieve the best improvement in the diarrhea rate and feed conversion ratio.

[0126] As shown by the above experiment, the addition of defatted ganoderma lucidum spore powder and compound probiotics in the diet of piglets can significantly improve the production performance of piglets, and the simultaneous addition of the two can obtain better production performance.

[0127] It is apparent for those skilled in the art that the present application is not limited to the details of the foregoing exemplary embodiments, and the present application can be implemented in other concrete forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be considered in all respects as illustrative and not restrictive, and the scope of the present application is defined by the appended claims rather than the foregoing description, and it is intended to encompass all changes falling within the meaning and range of equivalents of the claims.

Claims

1. A feed additive, characterized in that, The additive comprises defatted Ganoderma lucidum spore powder, Clostridium butyricum, and Bacillus coagulans. The additive provides the following components per kilogram of basal feed: defatted Ganoderma lucidum spore powder 50-200 mg; Clostridium butyricum 1 x 10 8 cfu ~ 10 x 10 8 cfu; Bacillus coagulans 1 x 10 9 ~ 10 x 10 9 cfu.

2. The feed additive according to claim 1, characterized in that, Also included is Bacillus licheniformis, 1 x 10 9 ~ 10 x 10 9 cfu Bacillus licheniformis per kg of base feed.

3. The feed additive according to claim 1, characterized in that, The additive provides the following components per kilogram of basal feed: defatted Ganoderma lucidum spore powder 50-200 mg; Clostridium butyricum 1 x 10 8 cfu ~ 10 x 10 8 cfu; Bacillus licheniformis 1 x 10 9 ~ 5 x 10 9 cfu; Bacillus coagulans 5 x 10 9 ~ 10 x 10 9 cfu.

4. A biological feed containing defatted Ganoderma lucidum spore powder and composite bacteria, characterized in that, The basal feed and premix are included; the premix comprises the feed additive as claimed in claim 1.

5. The bio-feed of claim 4, wherein, The premix provides the following components per kilogram of basal feed: Cu 90-120 mg; Zn 80-100 mg; Fe 110-150 mg; Mn 70-90 mg; Se 0.25-0.35 mg; I 0.1-0.2 mg.

6. The bio-feed of claim 4, wherein, The premix provides the following components per kilogram of basal feed: VA 1800-2200 IU; VD3 1350-1650 IU; VE 48-58 mg; VK3 0.9-1.1 mg; VB1 5.5-6.5 mg; VB2 2.5-3.1 mg; VB6 2.5-3.1 mg; VB 12 0.09-0.11 mg; Folic acid 1.8-2.2 mg; Niacin 7-9 mg; Pantothenic acid 25-31 mg; Biotin 0.18-0.22 mg.

7. The bio-feed of claim 4, wherein: The premix provides the following components per kilogram of basal feed: Phytase 450-550 U / kg; Acid protease 110-130 U / kg; Beta-glucanase 45-55 U / kg; Xylanase 140-16 U / kg.

8. The bio-feed of claim 4, wherein: The basal feed is pig feed, chicken feed, or duck feed.

9. Use of the feed additive as claimed in any one of claims 1 to 3 to prepare feed.

10. Use according to claim 9, characterized in that, The feed is pig feed, chicken feed, or duck feed.

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