Anti-wrinkle, whitening and freckle-removing skin care product and preparation method thereof

Abstract

The invention provides an anti-wrinkle, whitening and freckle-removing skin care product and a preparation method thereof, and belongs to the technical field of cosmetics. The skin care product comprises the following components: a phase A: shea butter, simmondsia chinensis seed oil, tocopherol, cetostearyl alcohol, isooctyl palmitate, squalane and polyglycerol-3 methyl glucose distearate; the phase B is prepared from glycerol, 1, 2-hexanediol, sodium ascorbate phosphate and disodium ethylene diamine tetraacetate; a phase C: pterostilbene, nicotinamide, an ethyl ferulate compound, a ginseng extract and a sea-buckthorn extract; phase D: methylparaben and a stabilizer; and the balance of deionized water. Through the synergistic effect of the raw materials, the prepared skin care product not only has the effects of removing wrinkles, whitening and removing freckles, but also is good in stability and high in safety.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to an anti-wrinkle, whitening, and freckle-removing skincare product and its preparation method. Background Technology

[0002] As people's living standards improve, more and more people are paying attention to skin health and anti-wrinkle, whitening, and blemish-removing treatments. Skin, the outermost layer of the human body, plays a vital physiological role. Its barrier function protects the body's organs and tissues from external damage. It is crucial for the body's adaptation to the environment and for maintaining internal homeostasis. Cosmetics not only achieve beauty goals but also protect the skin. Therefore, cosmetics with anti-wrinkle, whitening, blemish-removing, and moisturizing properties are becoming increasingly common.

[0003] Ethyl ferulic acid has physiological functions such as whitening, sun protection, and anti-aging, and is often added to skin care products as an active ingredient. However, ethyl ferulic acid is a fat-soluble component with extremely poor solubility in water and is unstable, easily decomposing and losing its activity due to factors such as light, pH, and temperature. Experiments have shown that the content of ethyl ferulic acid drops sharply within three days in areas exposed to sunlight, indicating that light has a significant impact on the stability of ethyl ferulic acid. Therefore, most commercially available products containing ethyl ferulic acid are packaged in dark or light-proof packaging. Ethyl ferulic acid exhibits relatively good stability and a slower degradation rate at lower pH levels. However, at higher pH levels, the degradation rate of ethyl ferulic acid accelerates significantly, and it easily generates colored products, leading to product color changes. The degradation rate of ethyl ferulic acid increases significantly with rising temperatures; therefore, products containing ethyl ferulic acid need to be stored in a low-temperature environment, and refrigeration is recommended. Due to these drawbacks, the application of ethyl ferulic acid in cosmetics is greatly limited. In addition, ethyl ferulic acid, as a fat-soluble ingredient, can irritate the skin at high concentrations and easily cause skin sensitivity, making it difficult to meet consumers' demand for efficient and safe skincare.

[0004] Furthermore, existing anti-wrinkle, whitening, and spot-removing cosmetics mostly use single-function ingredients, making it difficult to simultaneously address skin problems such as wrinkles, age spots, and dullness. Moreover, cosmetics are prone to deterioration or separation during long-term storage, affecting product quality. Therefore, developing skincare products with stable and safe ingredients, along with excellent anti-wrinkle, whitening, and spot-removing multiple functions, has gradually become an important research direction in the current cosmetics field. Summary of the Invention

[0005] In view of this, the present invention provides an anti-wrinkle, whitening, and freckle-removing skin care product, the skin care product comprising the following components:

[0006] Phase A: Shea butter 5-9%, jojoba seed oil 4-8%, tocopherol 0.03-0.05%, cetearyl alcohol 1-5%, isooctyl palmitate 2-5%, squalane 8-16%, polyglycerol-3-methylglucose distearate 5-8%;

[0007] Phase B: Glycerin 8-15%, 1,2-hexanediol 5-12%, sodium vitamin C phosphate 0.1-0.5%, disodium ethylenediaminetetraacetate 0.01-0.05%;

[0008] Phase C: Pterostilbene 0.01-0.07%, Nicotinamide 1-3%, Ethyl Ferulic Acid Complex 0.1-0.5%, Ginseng Extract 0.05-0.10%, Hippophae Rhamnoides Extract 0.05-0.10%;

[0009] Phase D: Methylparaben 0.5-0.8%, stabilizer 0.01-0.03%;

[0010] The rest is deionized water.

[0011] Preferably, the skincare product comprises the following components:

[0012] Phase A: Shea butter 6%, jojoba seed oil 5%, tocopherol 0.005%, cetearyl alcohol 2%, isooctyl palmitate 4%, squalane 12%, polyglycerol-3-methyl glucoside distearate 7%;

[0013] Phase B: Glycerin 11%, 1,2-hexanediol 8%, Sodium Vitamin C Phosphate 0.3%, Disodium Ethylenediaminetetraacetate 0.03%;

[0014] Phase C: Pterostilbene 0.05%, Nicotinamide 2%, Ethyl Ferulic Acid Complex 0.4%, Ginseng Extract 0.07%, Sea Buckthorn Extract 0.08%;

[0015] Phase D: Methylparaben 0.6%, stabilizer 0.02%;

[0016] The rest is deionized water.

[0017] Furthermore, the preparation method of the ethyl ferulic acid complex includes the following steps:

[0018] (1) Lecithin, ethyl ferulic acid, and cocoyl glucoside are mixed in a certain proportion to obtain a mixture. Then, the mixture is placed in 1,4-butanediol and heated at 65-80°C to dissolve it, thus obtaining an organic phase:

[0019] (2) Dissolve rhodioloside in deionized water to obtain an aqueous phase;

[0020] (3) After mixing the organic phase and the aqueous phase, homogenization was performed to obtain the ethyl ferulic acid complex.

[0021] Furthermore, the mass ratio of lecithin, ethyl ferulic acid, and cocoyl glucoside is 1.3-2.8:0.15-0.38:0.03-0.05, and preferably, the mass ratio of lecithin, ethyl ferulic acid, and cocoyl glucoside is 1.9:0.28:0.04.

[0022] Furthermore, the amount of 1,4-butanediol added accounts for 20-30% of the total mass of the system, preferably, the amount of 1,4-butanediol added accounts for 25% of the total mass of the system.

[0023] Furthermore, the mass fraction of rhodioloside in the aqueous phase is 0.2-0.7%, preferably, the mass fraction of rhodioloside in the aqueous phase is 0.6%.

[0024] Furthermore, the amount of deionized water added accounts for 65-75% of the total mass of the system, preferably, the amount of deionized water added accounts for 68% of the total mass of the system.

[0025] Furthermore, the homogenizer speed is 10000-15000 r / min, and the homogenization time is 2-5 min. Preferably, the homogenizer speed is 12000 r / min, and the homogenization time is 3 min.

[0026] Furthermore, the stabilizer is a wheat protein and nicotinamide mononucleotide complex.

[0027] Furthermore, the method for preparing the wheat protein and nicotinamide mononucleotide complex includes:

[0028] Wheat protein and nicotinamide mononucleotide were added to deionized water to obtain a suspension. The suspension was heated to 40-48℃ and reacted for 1-2.5 hours. The suspension was then cooled to room temperature to obtain a cooling solution. The cooling solution was freeze-dried to obtain a powdery substance, namely the wheat protein and nicotinamide mononucleotide complex.

[0029] Preferably, the method for preparing the wheat protein and nicotinamide mononucleotide complex includes:

[0030] Wheat protein and nicotinamide mononucleotide were added to deionized water to obtain a suspension. The suspension was heated to 45°C and reacted for 1 hour. The suspension was then cooled to room temperature to obtain a cooling solution. The cooling solution was freeze-dried to obtain a powdery substance, namely the wheat protein and nicotinamide mononucleotide complex.

[0031] Furthermore, the mass fraction of wheat protein in the suspension is 1.0-1.8%, preferably 1.5%.

[0032] Furthermore, the mass fraction of nicotinamide mononucleotide in the suspension is 0.5-0.7%, preferably, the mass fraction of nicotinamide mononucleotide in the suspension is 0.6%.

[0033] Furthermore, the freeze-drying temperature is -50 to -30°C, preferably -48°C.

[0034] This invention also provides a method for preparing the aforementioned anti-wrinkle, whitening, and freckle-removing skincare product, comprising the following steps:

[0035] (1) Mix shea butter, jojoba seed oil, tocopherol, cetearyl alcohol, isooctyl palmitate, squalane, and polyglycerol-3-methylglucose distearate in proportion, heat and stir to obtain mixed phase A, and set aside;

[0036] (2) Mix deionized water, glycerol, 1,2-hexanediol, sodium vitamin C phosphate, and disodium ethylenediaminetetraacetate in proportion, heat and stir to obtain mixed phase B, and set aside;

[0037] (3) Add the mixed phase A to the mixed phase B, homogenize, cool down, then add pterostilbene, nicotinamide, ethyl ferulic acid complex, ginseng extract, sea buckthorn extract, methylparaben, and stabilizer, and place in an ice bath for ultrasonic treatment to obtain the anti-wrinkle, whitening and spot-removing skin care product.

[0038] Furthermore, the heating and stirring temperature in step (1) is 70-80°C, preferably 75°C.

[0039] Furthermore, the heating and stirring temperature in step (2) is 82-88℃, and the cooling temperature in step (3) is 40-50℃. Preferably, the heating and stirring temperature in step (2) is 86℃, and the cooling temperature in step (3) is 45℃.

[0040] Furthermore, in step (3), the homogenization speed is 8000-10000 rpm and the homogenization time is 3-10 min. Preferably, in step (3), the homogenization speed is 9000 rpm and the homogenization time is 7 min.

[0041] Furthermore, in step (3), the ultrasonic power is 500-800W and the ultrasonic treatment time is 10-15min. Preferably, in step (3), the ultrasonic power is 700W and the ultrasonic treatment time is 13min.

[0042] Furthermore, the skincare products include any one of lotion, cream, toner, serum, and facial cleanser.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] The ethyl ferulic acid ester complex provided by this invention has good stability and is safe and skin-friendly, effectively reducing the risk of skin irritation and allergies caused by cosmetics.

[0045] The wheat protein and nicotinamide mononucleotide complex used in this invention form a complex through hydrogen bonding and a three-dimensional network structure in the continuous phase, thereby improving the stability of skin care products, avoiding the defects of easy separation or deterioration of cosmetics during long-term storage, and achieving the effect of extending the shelf life of skin care products.

[0046] The extracts of pterostilbene, ginseng, and sea buckthorn added in this invention have synergistic effects. By synergistically increasing the activity of cytochrome C oxidase, they improve cell function, increase the metabolic rate, and reduce oxidative damage, thereby achieving skin care effects such as whitening, anti-wrinkle, and spot removal.

[0047] The skincare products prepared by this invention utilize the synergistic effect between various raw materials and have excellent anti-wrinkle, whitening, and freckle-removing effects, as well as good stability and high safety. Detailed Implementation

[0048] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0049] Unless otherwise specified, the test methods or experimental methods described in the following examples are all conventional methods; unless otherwise specified, the raw materials and additives are obtained from conventional commercial sources or prepared by conventional methods.

[0050] The preparation methods of the ethyl ferulic acid complex in the following examples or comparative examples are as follows:

[0051] (1) The lecithin, ethyl ferulic acid, and cocoyl glucoside were mixed evenly in a mass ratio of 1.9:0.28:0.04 to obtain a mixture. Then, the mixture was placed in 1,4-butanediol, wherein the amount of 1,4-butanediol added accounted for 25% of the total mass of the system. The mixture was heated at 68°C to dissolve the mixture and obtain an organic phase.

[0052] (2) Rhodioloside is dissolved in deionized water, and the mass fraction of rhodioloside in the aqueous phase is 0.6%, to obtain the aqueous phase;

[0053] (3) After mixing the organic phase and the aqueous phase, homogenize at 12000 r / min for 3 min to obtain ethyl ferulic acid complex.

[0054] The preparation methods of the wheat protein and nicotinamide mononucleotide complex in the following examples or comparative examples are as follows:

[0055] 1.5 wt% wheat protein and 0.6 wt% nicotinamide mononucleotide were added to deionized water to obtain a suspension. The suspension was heated to 45 °C and reacted for 1 h. After the reaction was completed, the suspension was cooled to room temperature to obtain a cooling solution. The cooling solution was freeze-dried at -48 °C for 20 h to obtain a powdery substance, namely the wheat protein and nicotinamide mononucleotide complex.

[0056] The preparation method of sea buckthorn extract is as follows: 50g of sea buckthorn leaves are crushed and passed through an 80-mesh sieve. Then, 75% ethanol aqueous solution is added at a material-to-liquid ratio of 1:10. The mixture is extracted at 65℃ for 15h, filtered, and the ethanol is recovered by vacuum concentration to obtain extract a and filter residue a. Deionized water is added to filter residue a at a material-to-liquid ratio of 1:5. The mixture is extracted at 80℃ for 6h, filtered, and extract b is obtained. Extracts a and b are combined, freeze-dried at -50℃ for 3h, and then ultra-finely pulverized to obtain sea buckthorn extract powder.

[0057] The preparation method of ginseng extract is as follows: 120g of ginseng rhizome is washed, dried, pulverized and passed through an 80-mesh sieve. 75% ethanol aqueous solution is added at a material-to-liquid ratio of 1:5. The mixture is refluxed twice at 65℃ for 2 hours each time. The extracts are filtered and combined, and concentrated under reduced pressure to 15% of the original volume to obtain a concentrated extract. Then, deionized water is added to 200 mL, and the mixture is filtered to obtain a filtered ginseng extract. 80 mL of FL-2 adsorption resin is wet-packed into an adsorption column and soaked in 85% ethanol aqueous solution for 10 hours. Then, the mixture is gradually transferred to the aqueous phase. The filtered ginseng extract is passed through the adsorption column at a flow rate of 0.3 BV / h, and then eluted with deionized water and 75% ethanol at a flow rate of 0.5 BV / h. The eluent is collected, concentrated under reduced pressure until dry, and ginseng extract powder is obtained.

[0058] Rhodioloside (Rhodioloside), 98% purity, CAS No.: 10338-51-9, purchased from Xi'an Minglang Biotechnology Co., Ltd.

[0059] Example 1

[0060] An anti-wrinkle, whitening, and freckle-removing skincare product, the raw material composition of which is as follows:

[0061] Phase A: Shea butter 6%, jojoba seed oil 5%, tocopherol 0.05%, cetearyl alcohol 2%, isooctyl palmitate 4%, squalane 12%, polyglycerol-3-methyl glucoside distearate 7%;

[0062] Phase B: Glycerin 11%, 1,2-hexanediol 8%, Sodium Vitamin C Phosphate 0.3%, Disodium Ethylenediaminetetraacetate 0.03%;

[0063] Phase C: Pterostilbene 0.05%, Nicotinamide 2%, Ethyl Ferulic Acid Complex 0.4%, Ginseng Extract 0.07%, Sea Buckthorn Extract 0.08%;

[0064] Phase D: Methylparaben 0.6%, stabilizer 0.02%;

[0065] The rest is deionized water;

[0066] The preparation method of the anti-wrinkle, whitening, and freckle-removing skincare product includes the following specific steps:

[0067] (1) Mix shea butter, jojoba seed oil, tocopherol, cetearyl alcohol, isooctyl palmitate, squalane, and polyglycerol-3-methylglucose distearate in proportion, heat and stir at 75°C until fully dissolved to obtain mixed phase A, and set aside.

[0068] (2) Mix deionized water, glycerol, 1,2-hexanediol, sodium vitamin C phosphate and disodium ethylenediaminetetraacetate in proportion, heat and stir at 86°C until fully dissolved to obtain mixed phase B, and set aside.

[0069] (3) Add the mixed phase A to the mixed phase B, homogenize at 9000 rpm for 7 min, cool down to 45℃, then add pterostilbene, nicotinamide, ethyl ferulic acid complex, ginseng extract, sea buckthorn extract, methylparaben, and stabilizer, and place in an ice bath for 700W ultrasonic treatment for 13 min to obtain the anti-wrinkle, whitening and spot-removing skin care product.

[0070] Example 2

[0071] An anti-wrinkle, whitening, and freckle-removing skincare product, the raw material composition of which is as follows:

[0072] Phase A: Shea butter 5%, jojoba seed oil 4%, tocopherol 0.03%, cetearyl alcohol 1%, isooctyl palmitate 2%, squalane 8%, polyglycerol-3-methyl glucoside distearate 5%;

[0073] Phase B: Glycerin 8%, 1,2-hexanediol 5%, Sodium Vitamin C Phosphate 0.1%, Disodium Ethylenediaminetetraacetate 0.01%;

[0074] Phase C: Pterostilbene 0.01%, Nicotinamide 1%, Ethyl Ferulic Acid Complex 0.1%, Ginseng Extract 0.05%, Sea Buckthorn Extract 0.05%;

[0075] Phase D: 0.5% methylparaben, 0.01% wheat protein and nicotinamide mononucleotide complex stabilizer;

[0076] The rest is deionized water;

[0077] The preparation method of the anti-wrinkle, whitening, and freckle-removing skincare product includes the following specific steps:

[0078] (1) Mix shea butter, jojoba seed oil, tocopherol, cetearyl alcohol, isooctyl palmitate, squalane, and polyglycerol-3-methyl glucoside distearate in proportion, heat and stir at 70°C until fully dissolved to obtain mixed phase A, and set aside.

[0079] (2) Mix deionized water, glycerol, 1,2-hexanediol, sodium vitamin C phosphate and disodium ethylenediaminetetraacetate in proportion, heat and stir at 82°C until fully dissolved to obtain mixed phase B, and set aside for later use;

[0080] (3) Add the mixed phase A to the mixed phase B, homogenize at 8000 rpm for 3 min, cool down to 40℃, then add pterostilbene, nicotinamide, ethyl ferulic acid complex, ginseng extract, sea buckthorn extract, methylparaben, and stabilizer, place in an ice bath and sonicate at 500W for 10 min to obtain the anti-wrinkle, whitening and spot-removing skin care product.

[0081] Example 3

[0082] An anti-wrinkle, whitening, and freckle-removing skincare product, the raw material composition of which is as follows:

[0083] Phase A: Shea butter 9%, jojoba seed oil 8%, tocopherol 0.05%, cetearyl alcohol 5%, isooctyl palmitate 5%, squalane 16%, polyglycerol-3-methyl glucoside distearate 8%;

[0084] Phase B: Glycerin 15%, 1,2-hexanediol 12%, Sodium Vitamin C Phosphate 0.5%, Disodium Ethylenediaminetetraacetate 0.05%;

[0085] Phase C: Pterostilbene 0.07%, Nicotinamide 3%, Ethyl Ferulic Acid Complex 0.5%, Ginseng Extract 0.10%, Sea Buckthorn Extract 0.10%;

[0086] Phase D: Methylparaben 0.8%, wheat protein and nicotinamide mononucleotide complex stabilizer 0.03%;

[0087] The rest is deionized water;

[0088] The preparation method of the anti-wrinkle, whitening, and freckle-removing skincare product includes the following specific steps:

[0089] (1) Mix shea butter, jojoba seed oil, tocopherol, cetearyl alcohol, isooctyl palmitate, squalane, and polyglycerol-3-methylglucose distearate in proportion, heat and stir at 80°C until fully dissolved to obtain mixed phase A, and set aside.

[0090] (2) Mix deionized water, glycerol, 1,2-hexanediol, sodium vitamin C phosphate and disodium ethylenediaminetetraacetate in proportion, heat and stir at 88°C until fully dissolved to obtain mixed phase B, and set aside.

[0091] (3) Add the mixed phase A to the mixed phase B, homogenize at 10,000 rpm for 10 min, cool down to 50℃, then add pterostilbene, nicotinamide, ethyl ferulic acid complex, ginseng extract, sea buckthorn extract, methylparaben, and stabilizer, and place in an ice bath for 800W ultrasonic treatment for 15 min to obtain the anti-wrinkle, whitening and spot-removing skin care product.

[0092] Comparative Example 1

[0093] The difference from Example 1 is that it does not contain pterostilbene.

[0094] Comparative Example 2

[0095] The difference from Example 1 is that it does not contain the ethyl ferulic acid ester complex.

[0096] Comparative Example 3

[0097] The difference from Example 1 is that an equal amount of ethyl ferulic acid was used to replace the ethyl ferulic acid complex.

[0098] Comparative Example 4

[0099] The difference from Example 1 is that it does not contain rhodioloside.

[0100] Comparative Example 5

[0101] The difference from Example 1 is that it does not contain ginseng extract or sea buckthorn extract.

[0102] Comparative Example 6

[0103] The difference from Example 1 is that it does not contain stabilizers.

[0104] Comparative Example 7

[0105] The difference from Example 1 is that an equal amount of soy protein is used to replace wheat protein.

[0106] Comparative Example 8

[0107] The difference from Example 1 is that no ultrasonic treatment was performed.

[0108] 1) Safety evaluation

[0109] Skin irritation test: Ten mice were selected, and the hair on their backs was shaved. The cosmetic product provided in Example 1 was used as a sample and adhered to the back of the mouse using non-irritating adhesive tape. The sample was then gently pressed by hand to ensure even application to the skin. After 72 hours, the sample was removed, and the mice were observed for any irritant reactions such as erythema, itching, papules, or vesicles. There were no statistically significant differences in sex, age, weight, or body length among the selected mice (P > 0.05).

[0110] Rating:

[0111] Grade 1: No erythema, itching, papules or vesicles appeared on the skin in the test area.

[0112] Grade 2: Mild erythema and / or mild itching appeared on the skin in the test area, without papules or vesicles.

[0113] Grade 3: Obvious erythema and / or significant itching appeared on the skin in the test area, with occasional papules and vesicles.

[0114] Grade 4: Obvious erythema and / or obvious itching, and / or obvious papules and vesicles appear on the skin in the test area.

[0115] The reaction results were recorded according to the above standards. The test results showed that after 72 hours of irritation test on mice, no erythema, itching, papules or vesicles appeared on the skin of the test area, indicating that the anti-wrinkle, whitening and freckle-removing skin care product provided by the present invention is safe and non-irritating.

[0116] Testing showed that the anti-wrinkle, whitening, and freckle-removing skincare products provided in Examples 2-3 were also safe and non-irritating, and their effects were not significantly different from those in Example 1.

[0117] The skincare products provided by Comparative Examples 1-2 and 4-8 were safe and non-irritating, but the skincare product provided by Comparative Example 3 was less safe. This indicates that modifying ethyl ferulic acid helps improve its safety and reduce the risk of skin irritation and allergies.

[0118] II) Stability Evaluation

[0119] Room temperature observation: Weigh 30g of the anti-wrinkle, whitening and freckle-removing skin care product provided in Example 1 as the test sample and place it in a sealed container. Place it at room temperature for 2 months and observe its properties. Repeat the test 3 times for each test sample.

[0120] Heat resistance test: Weigh 30g of the test sample and place it at 45℃ for 36h. After it returns to room temperature, observe whether there are any changes in the properties of the test sample.

[0121] Cold resistance test: Weigh 30g of the test sample and place it at -25℃ for 36h. After it returns to room temperature, observe whether there are any changes in the properties of the test sample.

[0122] Centrifugation test: Weigh 30g of the test sample and place it in a centrifuge tube. Centrifuge at 2000r / min for 20min at 25℃ and observe whether there are any changes in the properties of the test sample.

[0123] The stability evaluation results showed that there was no significant difference in the properties of the tested samples and no stratification was observed, indicating that the anti-wrinkle, whitening and freckle-removing skin care product provided in Example 1 of the present invention has good stability.

[0124] Testing showed that the anti-wrinkle, whitening, and freckle-removing skincare products provided in Examples 2-3 also exhibited good stability, and their effects were not significantly different from those in Example 1.

[0125] The skincare products provided by Comparative Examples 1-2 and 4-5 showed good stability, but the skincare products provided by the other comparative examples showed poor stability. This indicates that the addition of a specific ethyl ferulic acid complex and stabilizer, as well as ultrasonic treatment, helps to improve the storage stability of the skincare products and extend their shelf life.

[0126] III) Evaluation of Whitening Efficacy

[0127] According to the "Cosmetics - Tyrosinase Activity Inhibition Test Method, T / SHRH 015-2018", the inhibition rate (%) of tyrosinase in the cosmetics prepared in the examples and comparative examples was tested. The higher the inhibition rate of tyrosinase, the better the whitening effect of the "Noble Lady Cream".

[0128] IV) Evaluation of the skin-removing efficacy

[0129] Subjects: Female volunteers aged 18-55 years with facial pigmentation were randomly divided into 11 groups of 10 each. There were no statistically significant differences in pigmentation size, severity, and average age among the groups (P>0.05), indicating comparability.

[0130] Test instrument: German CK MPA6 multi-probe skin analyzer, Mexameter MX18 (melanin and hemoglobin test probes), to test the melanin content of the skin.

[0131] Test method: After cleansing the face every morning and evening, apply the cosmetic prepared in the example or comparative example. After 60 days of continuous use, test the melanin content on the volunteers' faces. The ambient temperature during the test was 25±1℃ and the humidity was 55±3%. Before the test, wash the face with water and dry it. Select a 3*3cm area in the middle of the forehead, the middle of the right cheek and the middle of the left cheek. Use a probe to test the average melanin content in this area and calculate the average melanin content of each group.

[0132] (v) Evaluation of wrinkle-reducing efficacy

[0133] Subjects: Female volunteers aged 18-55 with facial wrinkles were randomly divided into 11 groups of 10 each. There were no statistically significant differences in the severity of wrinkles and average age among the groups (P>0.05), indicating comparability.

[0134] Experimental instruments: CCD test sensor, sinusoidal stripe projector, PRIMOSlite rapid 3D skin imaging system.

[0135] Test Method: After cleansing the face every morning and evening, apply the cosmetic prepared in the examples or comparative examples. After 60 days of continuous use, test the wrinkles on the volunteers' faces. The ambient temperature during the test was 25±1℃ and the humidity was 55±3%. Before the test, wash the face with water and dry it. Select the outer corner of the eye area as the test area. Illuminate the test area with a sinusoidal stripe projector and use a CCD test sensor to collect three-dimensional image data of the area. Process the collected data through the PRIMOS lite skin rapid three-dimensional imaging system and calculate the average wrinkle shrinkage rate through the Overlay virtual image overlap test method.

[0136] The results of the whitening, spot removal, and wrinkle reduction tests are shown in Table 1.

[0137] Table 1

[0138] Group Tyrosinase inhibition rate (%) Average melanin content Average wrinkle shrinkage rate (%) Example 1 93 95 63 Example 2 90 103 62 Example 3 86 107 60 Comparative Example 1 71 138 39 Comparative Example 2 73 124 42 Comparative Example 3 77 116 45 Comparative Example 4 75 138 40 Comparative Example 5 69 141 36 Comparative Example 6 85 109 60 Comparative Example 7 83 108 62 Comparative Example 8 77 120 55

[0139] As shown in the table above, the addition of pterostilbene, ethyl ferulic acid complex, ginseng extract, and sea buckthorn extract, along with ultrasonic treatment, can effectively enhance the whitening, wrinkle-reducing, and spot-removing effects of skincare products.

[0140] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An anti-wrinkle, whitening, and freckle-removing skincare product, characterized in that, The skincare product includes the following components: Phase A: Shea butter 5-9%, jojoba seed oil 4-8%, tocopherol 0.03-0.05%, cetearyl alcohol 1-5%, isooctyl palmitate 2-5%, squalane 8-16%, polyglycerol-3-methylglucose distearate 5-8%; Phase B: Glycerin 8-15%, 1,2-hexanediol 5-12%, sodium vitamin C phosphate 0.1-0.5%, disodium ethylenediaminetetraacetate 0.01-0.05%; Phase C: Pterostilbene 0.01-0.07%, Nicotinamide 1-3%, Ethyl Ferulic Acid Complex 0.1-0.5%, Ginseng Extract 0.05-0.10%, Hippophae Rhamnoides Extract 0.05-0.10%; Phase D: Methylparaben 0.5-0.8%, stabilizer 0.01-0.03%; The rest is deionized water.

2. The anti-wrinkle, whitening, and freckle-removing skincare product according to claim 1, characterized in that, The preparation method of the ethyl ferulic acid complex includes the following steps: (1) Lecithin, ethyl ferulic acid, and cocoyl glucoside are mixed in a certain proportion to obtain a mixture. Then, the mixture is placed in 1,4-butanediol and heated at 65-80°C to dissolve it, thus obtaining an organic phase: (2) Dissolve rhodioloside in deionized water to obtain an aqueous phase; (3) After mixing the organic phase and the aqueous phase, homogenization was performed to obtain the ethyl ferulic acid complex.

3. The anti-wrinkle, whitening, and freckle-removing skincare product according to claim 2, characterized in that, The mass ratio of lecithin, ethyl ferulic acid, and cocoyl glucoside is 1.3-2.8:0.15-0.38:0.03-0.

05.

4. The anti-wrinkle, whitening, and freckle-removing skincare product according to claim 2, characterized in that, The amount of 1,4-butanediol added accounts for 20-30% of the total mass of the system, the mass fraction of rhodioloside in the aqueous phase is 0.2-0.7%, the amount of deionized water added accounts for 65-75% of the total mass of the system, the homogenizer speed is 10000-15000 r / min, and the homogenization time is 2-5 min.

5. The anti-wrinkle, whitening, and freckle-removing skincare product according to claim 1, characterized in that, The stabilizer is a wheat protein and nicotinamide mononucleotide complex.

6. The anti-wrinkle, whitening, and freckle-removing skincare product according to claim 5, characterized in that, The method for preparing the wheat protein and nicotinamide mononucleotide complex includes: Wheat protein and nicotinamide mononucleotide were added to deionized water to obtain a suspension. The suspension was heated to 40-48℃ and reacted for 1-2.5 hours. The suspension was then cooled to room temperature to obtain a cooling solution. The cooling solution was freeze-dried to obtain a powdery substance, namely the wheat protein and nicotinamide mononucleotide complex.

7. The anti-wrinkle, whitening, and freckle-removing skincare product according to claim 6, characterized in that, The suspension contains 1.0-1.8% wheat protein by mass, 0.5-0.7% nicotinamide mononucleotide by mass, and the freeze-drying temperature is -50 to -30°C.

8. The method for preparing the anti-wrinkle, whitening, and freckle-removing skincare product according to any one of claims 1-7, characterized in that, Includes the following steps: (1) Mix shea butter, jojoba seed oil, tocopherol, cetearyl alcohol, isooctyl palmitate, squalane, and polyglycerol-3-methylglucose distearate in proportion, heat and stir to obtain mixed phase A, and set aside; (2) Mix deionized water, glycerol, 1,2-hexanediol, sodium vitamin C phosphate, and disodium ethylenediaminetetraacetate in proportion, heat and stir to obtain mixed phase B, and set aside; (3) Add the mixed phase A to the mixed phase B, homogenize, cool down, then add pterostilbene, nicotinamide, ethyl ferulic acid complex, ginseng extract, sea buckthorn extract, methylparaben, and stabilizer, and place in an ice bath for ultrasonic treatment to obtain the anti-wrinkle, whitening and spot-removing skin care product.

9. The preparation method of the anti-wrinkle, whitening, and freckle-removing skincare product according to claim 8, characterized in that, The heating and stirring temperature in step (1) is 70-80℃, the heating and stirring temperature in step (2) is 82-88℃, and the cooling temperature in step (3) is 40-50℃.

10. The preparation method of the anti-wrinkle, whitening, and freckle-removing skincare product according to claim 8, characterized in that, In step (3), the homogenization speed is 8000-10000 rpm, the homogenization time is 3-10 min, the ultrasonic power is 500-800 W, and the ultrasonic treatment time is 10-15 min.