State monitoring system and method for rear light system

Abstract

The invention discloses a state monitoring system of a rear light system. The state monitoring system comprises a controller, a motor driver, a front light signal generator and a detection circuit, the controller is connected with the motor driver and used for responding to a monitoring instruction for the rear light system, controlling the motor driver, moving the front light signal generator installed at the position of an output shaft to a light path of the front light system and the rear light system and enabling a light emitting device of the front light signal generator to face an optical fiber head of the rear light system; the front optical signal generator is used for outputting Gaussian pulse optical signals which are the same as those of the front optical system to the optical fiber head; and the detection circuit is connected with the optical fiber head through an optical fiber and is used for converting the received Gaussian pulse optical signal into a voltage signal and determining a state monitoring result of the rear optical system based on the size of the voltage signal.

Description

Technical Field

[0001] This application relates to the field of detection technology, and in particular to a state monitoring system and method for a post-lighting system. Background Technology

[0002] Flow cytometers, especially spectroscopic flow cytometers, are very complex systems. During use, some problems inevitably occur with the instrument. If the systems before and after the cytometer are tested together, the detection process becomes complicated and inefficient. Summary of the Invention

[0003] In view of this, embodiments of this application provide at least one post-light system state monitoring system and method.

[0004] The technical solution of this application embodiment is implemented as follows:

[0005] This application provides a status monitoring system for a rear light system, which includes a controller, a motor driver, a front light signal generator, and a detection circuit.

[0006] The controller, connected to the motor driver, is used to control the motor driver in response to monitoring commands for the rear optical system, to move the front optical signal generator mounted on the output shaft to the optical path between the front and rear optical systems, and to orient the light-emitting device of the front optical signal generator toward the fiber optic head of the rear optical system.

[0007] The front optical signal generator is used to output the same Gaussian pulse optical signal as the front optical system to the fiber optic head;

[0008] The detection circuit, connected to the fiber optic connector via an optical fiber, is used to convert the received Gaussian pulse optical signal into a voltage signal, and to determine the state monitoring result of the optical system based on the magnitude of the voltage signal.

[0009] In the aforementioned state monitoring system of the rear optical system, the controller, connected to the front optical signal generator, is also used to acquire the signal characteristic information of the optical signal emitted by the microsphere in the front optical system, and control the front optical signal generator to output a Gaussian pulse optical signal with the same signal characteristic information.

[0010] In the aforementioned post-light system status monitoring system, the front-light signal generator also includes a voltage output circuit and a light signal driver; a controller, connected to the voltage output circuit, for controlling the voltage output circuit to output a Gaussian pulse voltage signal with the same signal characteristics; a light signal driver, connected to the voltage output circuit, for receiving the Gaussian pulse voltage signal output by the voltage output circuit and linearly converting the Gaussian pulse voltage signal into a Gaussian pulse current signal; and a light-emitting device, connected to the light signal driver, for linearly converting the Gaussian pulse current signal into a Gaussian pulse light signal output.

[0011] In the aforementioned post-optical system status monitoring system, the light-emitting device is a device capable of emitting light signals covering the entire wavelength band.

[0012] In the aforementioned post-optical system's status monitoring system, the detection circuit includes at least one detector; each of the at least one detector is connected to an optical fiber head via a corresponding optical fiber, and is used to receive Gaussian pulse optical signals of the corresponding wavelength band and convert the Gaussian pulse optical signals of the corresponding wavelength band into corresponding voltage signals; each detector is also used to determine the corresponding detector status result based on the magnitude of the corresponding voltage signal.

[0013] In the aforementioned post-optical system status monitoring system, the controller, connected to the motor driver, is used to control the motor driver to send the Gaussian pulse optical signal emitted by the front optical signal generator into the fiber optic head in response to the detection circuit not receiving the optical signal sent by the front optical system, so as to obtain the status monitoring result of the post-optical system based on the magnitude of the voltage signal determined by the detection circuit.

[0014] In the aforementioned post-light system status monitoring system, a photodiode is installed in the detector; the photodiode is equipped with a calibration bias voltage to fix the gain of the photodiode to the target gain, and the photodiode operates in the linear operating region.

[0015] In the aforementioned status monitoring system of the rear light system, the front light signal generator includes a temperature control circuit; the temperature control circuit is connected to the light-emitting device and is used to stabilize the output power of the light-emitting device.

[0016] This application provides a method for monitoring the state of a rear optical system, applied to a state monitoring system for a rear optical system. The state monitoring system includes a controller, a motor driver, a front optical signal generator, and a detection circuit. The controller is connected to the motor driver. The detection circuit is connected to the fiber optic connector via an optical fiber. In response to a monitoring command for the rear optical system, the controller controls the motor driver to move the front optical signal generator, mounted on its output shaft, to the optical path between the front and rear optical systems, and to orient the light-emitting device of the front optical signal generator toward the fiber optic connector of the rear optical system. The front optical signal generator outputs the same Gaussian pulse light signal as the front optical system to the fiber optic connector. The detection circuit converts the received Gaussian pulse light signal into a voltage signal and determines the state monitoring result of the rear optical system based on the magnitude of the voltage signal.

[0017] In the above-mentioned state monitoring method of the rear optical system, the controller is connected to the front optical signal generator; the controller acquires the signal characteristic information of the optical signal emitted by the microsphere in the front optical system, and controls the front optical signal generator to output a Gaussian pulse optical signal with the same signal characteristic information.

[0018] It should be understood that the above general description and the following detailed description are merely exemplary and explanatory, and are not intended to limit the technical solutions of this application. Attached Figure Description

[0019] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with this application and, together with the specification, serve to explain the technical solutions of this application.

[0020] Figure 1 This is a schematic diagram of an exemplary instrument including a post-light system provided by related technologies;

[0021] Figure 2 This is a schematic diagram of an exemplary state monitoring system for a post-light system provided in an embodiment of this application. Figure 1 ;

[0022] Figure 3 This is a schematic diagram of an exemplary state monitoring system for a post-light system provided in an embodiment of this application. Figure 2 ;

[0023] Figure 4 This is a schematic diagram of an exemplary state monitoring system for a post-light system provided in an embodiment of this application. Figure 3 ;

[0024] Figure 5 This is a schematic diagram of an exemplary state monitoring system for a post-light system provided in an embodiment of this application. Figure 4 ;

[0025] Figure 6 This is a flowchart illustrating an exemplary status monitoring method provided in an embodiment of this application. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this application clearer, the technical solutions of this application are further described in detail below with reference to the accompanying drawings and embodiments. The described embodiments should not be regarded as limitations on this application. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0027] In the following description, references are made to “some embodiments,” which describe a subset of all possible embodiments. However, it is understood that “some embodiments” may be the same subset or different subsets of all possible embodiments and may be combined with each other without conflict.

[0028] The terms “first / second / third” are used merely to distinguish similar objects and do not represent a specific ordering of objects. It is understood that “first / second / third” may be interchanged in a specific order or sequence where permitted, so that the embodiments of this application described herein can be implemented in an order other than that illustrated or described herein.

[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains. The terminology used herein is for descriptive purposes only and is not intended to limit the scope of this application.

[0030] like Figure 1 As shown, the optical system of the flow cytometer 10 can be simply divided into a front optical system 11 and a rear optical system 12. The front optical system 11 includes a laser 111, a laser shaping 112, a flow chamber 113, a forward scattering detector 114, and a lens 115. The rear optical system 12 includes an optical fiber head 121, an optical fiber 122, and a detector 123. The light signal emitted by the laser 111 undergoes forward scattering and lateral scattering after passing through the laser shaping 112 and the flow chamber 113. The forward-scattered light then enters the receiver of the forward scattering detector 114, while the lateral scattering passes through the lens 115 to reach the rear optical system 12. The rear optical system 12 receives the light signal from the front optical system 11 and converts it into an electrical signal to achieve cell detection.

[0031] In cytometers, especially in spectroscopic flow cytometers, the back light system 12 has a large number of detection channels, particularly the five-laser spectroscopic flow cytometer with nearly 80 channels. If the front light system 11 and the back light system 12 are monitored together, the monitoring of the cytometer becomes quite complex. For these reasons, there is a need for a status monitoring system and method for the back light system of a flow cytometer.

[0032] This application provides a status monitoring system for a post-light system. Figure 2 This is a schematic diagram of the structure of a front light system state monitoring system 20 provided in an embodiment of this application.

[0033] like Figure 2As shown, the status detection system 20 includes a controller 21, a motor driver 22, a front optical signal generator 23, and a detection circuit 24. The controller 21, connected to the motor driver 22, is used to control the motor driver 22 in response to a monitoring command for the rear optical system 12, to move the front optical signal generator 23, which is mounted at the output shaft position, to the optical path between the front optical system 11 and the rear optical system 12, and to orient the light-emitting device 25 of the front optical signal generator 23 toward the fiber optic head 121 of the rear optical system 12. The front optical signal generator 23 is used to output the same Gaussian pulse light signal as the front optical system 11 to the fiber optic head 121. The detection circuit 24, connected to the fiber optic head 121 via the fiber optic cable 122, is used to convert the received Gaussian pulse light signal into a voltage signal and determine the status monitoring result of the rear optical system 12 based on the magnitude of the voltage signal.

[0034] In the embodiments of this application, if the cell analyzer 10 is set to perform a power-on self-test, then once the cell analyzer 10 is turned on, it is considered that it has received a monitoring command for the back-light system 12. Alternatively, the back-light system 12 can perform a self-test after a preset time. For example, in addition to the self-test that can be performed immediately upon power-on as discussed above, the quality control can also be set to three months, two weeks, one month, or one week based on the lifespan of the fiber optic connector 121, fiber optic cable 122, or detection circuit 24 in the back-light system 12, or based on experience regarding how long it may be used before problems occur. For example, the timing of quality control monitoring can be set based on actual needs and application scenarios, and this application does not limit this. If a preset time is set, then when the preset time is reached, it is considered that a monitoring command for the back-light system 12 has been received.

[0035] In the embodiments of this application, the controller 21 may be a microcontroller unit (MCU), which can realize automated control by running pre-programmed instructions. A microcontroller is a microcomputer system that integrates key functional modules such as a central processing unit (CPU), memory (RAM / ROM), input / output interfaces (such as GPIO, UART, I2C, SPI), timers, analog-to-digital converters (ADCs), and digital-to-analog converters (DACs) on a single chip. The devices included in the controller in the embodiments of this application can be set according to actual needs, and this application does not limit them.

[0036] In the embodiments of this application, the motor driver 22 can be a servo motor, a DC motor, a stepper motor, or a servo motor, etc., and the type of motor driver 22 can be selected based on factors such as actual needs, control accuracy, speed performance, and torque. For example, if the motor driver 22 is a servo motor, the controller 21 can control the rotation angle of the servo motor through a PWM (Pulse Width Modulation) signal. For instance, after the servo motor receives a signal with a specific pulse width sent by the controller 21, the control circuit inside the servo motor compares the input signal and the reference signal to control the output shaft to reach the specified position and rotate to the target angle. If the input signal and the reference signal are inconsistent, the position and rotation angle of the output shaft need to be adjusted until the specified position and rotation angle corresponding to the reference signal are reached.

[0037] In this embodiment, a front light signal generator 23 can be installed at the output shaft position of the motor driver 22. If a monitoring command for the rear light system 12 is received, to avoid the high complexity of monitoring both the front light system 11 and the rear light system 12 together, a front light signal generator 23 is provided to output the same Gaussian pulse light signal as the front light system 11. Thus, after receiving the monitoring command, the controller 21 can control the motor driver 22 to move the front light signal generator 23 installed at the output shaft position into the optical path of the front light system 11 and the rear light system 12 to block the front light system 11. The light sent from the optical system 11 to the rear optical system 12 then directs the light-emitting device 25 of the front optical signal generator 23 toward the fiber optic head 121 of the rear optical system 12, so that the Gaussian pulse light signal emitted by the front optical signal generator 23, which is the same as that of the front optical system 11, is sent into the fiber optic head 121. In this way, even if the front optical system 11 fails, the Gaussian pulse light signal simulated by the front optical signal generator 23 is a standard light signal, indicating that the light signal entering the fiber optic head 121 is not a problem. Thus, if the monitoring results of the detection circuit 24 show a problem, it can be said that the problem lies with the rear optical system 12.

[0038] In the embodiments of this application, the fiber optic head 121 is a fiber optic connector, which is a key component for realizing a detachable connection between the fiber optic cable and the device. The fiber optic head 121 includes a single-mode fiber optic connector, a multimode fiber optic connector, or other types of fiber optic heads, which can be set according to actual needs and application scenarios, but it needs to be matched with the fiber optic cable 122.

[0039] In the embodiments of this application, the optical fiber 122 can be a single-mode fiber, a multi-mode fiber, or other fiber types. It can be set according to actual needs and application scenarios and matched with the aforementioned fiber optic head 121 to ensure that the optical signal can reach the optical fiber 122 through the fiber optic head 121. In this way, when the Gaussian pulse optical signal enters the optical fiber 122 through the fiber optic head 121, if the incident angle is greater than the critical angle when the optical signal enters the low refractive index cladding from the high refractive index fiber core, total internal reflection occurs, and the optical signal is confined to propagate within the fiber core to reach the detection circuit 24. The detection circuit 24 is connected to the fiber optic head 121 through the optical fiber 122 to receive the Gaussian pulse optical signal, and then converts the Gaussian pulse optical signal into a voltage signal. Based on the magnitude of the voltage signal, the state detection result of the optical system 12 is determined.

[0040] In this way, by simulating the optical signal of the front optical system, the monitoring of the front optical system and the rear optical system are separated. Furthermore, since the optical signal input to the rear optical system is based on the Gaussian pulse signal output by the front optical signal generator that is consistent with the front optical system, the standard of the optical signal input to the rear optical system can be guaranteed. This improves both the accuracy and efficiency of the rear optical system monitoring.

[0041] In some embodiments, such as Figure 2 As shown, the controller 21 is connected to the front light signal generator 23 and is also used to acquire the signal characteristic information of the light signal emitted by the microsphere in the front light system 11, and control the front light signal generator 23 to output a Gaussian pulse light signal with the same signal characteristic information.

[0042] In the embodiments of this application, the controller 21 is also connected to the front light signal generator 23 to obtain the signal characteristic information of the light signal emitted by the running microsphere in the front light system 11. For example, the signal characteristic information includes characteristic information such as amplitude, pulse width, and coefficient of variation (CV).

[0043] In the embodiments of this application, the microspheres generate a Gaussian beam in the cell analyzer 10 through a dot matrix light source, analyze the interference pattern of adjacent beams, and reconstruct the phase distribution of the sample. Generally, the quality requirements for the microspheres are high and the price is relatively expensive. Therefore, the Gaussian pulse light signal directly emitted by the front light signal generator 23 with the same signal characteristic information can ensure the accuracy of the Gaussian pulse light signal input to the rear light system 12 on the one hand, and reduce the cost on the other hand.

[0044] In the embodiments of this application, signal characteristic information of an optical signal is acquired by way of example: an optical power meter can be used to measure the average power; a photodetector is connected to an oscilloscope to capture a single pulse waveform, and the peak-to-peak value (Vpp) and pulse width are measured; the pulse width is measured 50 times repeatedly, and the CV is calculated to evaluate stability; the pulse waveform diagram and CV statistical results are plotted based on the data. In this way, signal characteristic information, amplitude, pulse width, and CV can be obtained.

[0045] In the embodiments of this application, when the controller 21 acquires the optical signal of the front optical system 11, it needs to ensure that the front optical system 11 can work normally. In this way, the signal feature information acquired based on the optical signal of the normal front optical system 11 will be accurate. If the front optical system 11 is not normal, the optical signal it emits will also be abnormal. Then, the accuracy of the state detection of the rear optical system 12 based on the Gaussian pulse optical signal generated based on the abnormal optical signal will also be reduced. Therefore, it is necessary to ensure that the optical signal acquired by the front optical system 11 is a standard optical signal, so as to ensure the accuracy of the Gaussian pulse optical signal emitted by the front optical signal generator 23 that is consistent with it.

[0046] In the embodiments of this application, if the signal characteristic information of the light signal emitted by the microsphere in the front light system 11 is obtained, the front light signal generator 23 can be programmed to output a Gaussian pulse light signal with the same signal characteristic information. The Gaussian pulse light signal can be obtained by running the programmed output.

[0047] In the embodiments of this application, the controller 21 can obtain the signal characteristic information of the light signal emitted by the microsphere in the front light system 11, and then write a program to output a light signal with the same signal characteristic information. Based on the program, the front light signal generator 23 outputs a Gaussian pulse light signal with the same signal characteristic information.

[0048] This allows for the accurate and easy generation of the output Gaussian pulse light signal.

[0049] In some embodiments, such as Figure 3 As shown, the front optical signal generator 23 also includes a voltage output circuit 27 and an optical signal driver 28; a controller 21, connected to the voltage output circuit 27, is used to control the voltage output circuit 27 to output a Gaussian pulse voltage signal with the same signal characteristics; an optical signal driver 28, connected to the voltage output circuit 27, is used to receive the Gaussian pulse voltage signal output by the voltage output circuit 27 and linearly convert the Gaussian pulse voltage signal into a Gaussian pulse current signal; and a light-emitting device 25, connected to the optical signal driver 28, is used to linearly convert the Gaussian pulse current signal into a Gaussian pulse optical signal output.

[0050] In embodiments of this application, the front optical signal generator 23 further includes a voltage output circuit 27, which can be a Direct Digital Synthesizer (DDS). The controller 21 writes a program to directly supply the DDS, causing the DDS to output a Gaussian pulse voltage signal with the same signal characteristics. The DDS is a technology that digitally generates analog signals, its core being phase accumulation and lookup table conversion. An exemplary procedure is as follows: Under clock drive, the phase accumulator increments by a fixed step size (frequency control word K) to generate a linear phase value; the phase value is used as an address to read the corresponding digital amplitude value (such as a sine wave) from a waveform lookup table (ROM); the digital-to-analog converter (DAC) converts the digital amplitude into an analog signal, and after filtering out high-frequency noise by a low-pass filter (LPF), a smooth waveform is output. Thus, the program written by the controller 21 is a digital voltage signal, which is used by the DDS to generate an analog voltage signal.

[0051] In the embodiments of this application, the controller 21 controls the voltage output circuit 27 to output a Gaussian pulse voltage signal with the same signal characteristics. Then, the Gaussian pulse voltage signal with the same signal characteristics is input to the optical signal driver 28, which linearly converts the Gaussian pulse voltage signal into a Gaussian pulse current signal. Subsequently, the light-emitting device 25 linearly converts the Gaussian pulse current signal into a Gaussian pulse light signal for output. Since the Gaussian pulse voltage signal output by the voltage output circuit 27, the Gaussian pulse current signal output by the optical signal driver 28, and the Gaussian pulse light signal output by the light-emitting device 25 are all linear conversions of the input signals, the Gaussian pulse light signal output by the light-emitting device 25, like the voltage signal input to the voltage output circuit 27, is also a Gaussian pulse light signal with adjustable amplitude and pulse width. Because adjusting the program in the controller 21 can adjust the voltage signal of the voltage output circuit 27, and since the entire data transmission is linear, the Gaussian pulse light signal output by the light-emitting device 25 can also be adjusted.

[0052] In the embodiments of this application, the optical signal driver 28 may be an LED (Light Emitting Diode) driver, which may include a DDS voltage signal buffer and amplification circuit, an LED constant current control circuit, and LED soft start protection circuits, etc. The DDS voltage signal buffer and amplification circuit can generate high-precision dimming or modulation signals (such as PWM waveforms) for dynamically adjusting LED brightness or achieving special effects (such as gradients and strobe). It isolates the DDS signal source from the subsequent load, preventing LED load changes (such as current fluctuations) from backflowing to the signal source, ensuring signal stability. It can also amplify the weak signal generated by DDS (such as 0-3.3V) to a voltage sufficient to drive the constant current control circuit (such as 0-10V), or directly adjust the LED's supply voltage. The LED constant current control circuit keeps the current through the LED constant, avoiding current overload or brightness instability caused by input voltage fluctuations or changes in the LED's forward voltage (Vf). An exemplary implementation can be achieved by adjusting the MOSFET / transistor on-resistance through a current sensing resistor and operational amplifier feedback. The LED soft-start protection circuit can prevent a large current surge to the LED or power module at power-on by controlling the power supply rise rate (such as using a capacitor or thyristor).

[0053] For example, the MCU (controller 21) controls the DDS (voltage output circuit 27) to output a Gaussian pulse voltage signal with adjustable amplitude and pulse width. This signal is input to the LED driver (light signal driver 28) as the modulation signal for the LED. The LED driver linearly converts the voltage signal output by the DDS into a current signal to drive the LED. Therefore, the final light signal output by the LED (light-emitting device 25) is essentially linearly related to the Gaussian signal controlled by the MCU's DDS, i.e., it is a Gaussian pulse light signal with adjustable amplitude and pulse width.

[0054] Thus, by writing a program to output a light signal that is essentially the same as that of a flow cytometer when running microspheres: with amplitude, pulse width, and CV all within 1%, a Gaussian pulse light signal with the same effect as a post-light system can be provided without running microspheres with an instrument.

[0055] In some embodiments, such as Figure 4 As shown, the light-emitting device 25 includes multiple light-emitting diodes 251, and the light signals emitted by the multiple light-emitting diodes 251 can cover the entire wavelength band.

[0056] In the embodiments of this application, since the detection circuit 24 in the backlight system 12 detects light signals of different wavelengths, the light-emitting device 25 needs to be a device that can emit light signals covering the entire wavelength range. Thus, the light-emitting device 25 may include multiple light-emitting diodes (LEDs) 251, or it may be an OLED (organic light-emitting diode) 251. The light signals emitted by the multiple light-emitting diodes 251 can cover the entire wavelength range. For example, the full wavelength range is 350nm-800nm. Among the multiple light-emitting diodes 251, there may be diodes that emit light signals of 350nm-450nm, 450nm-600nm, and 600nm-800nm. Of course, among the multiple light-emitting diodes 251, there may also be diodes that emit light signals of 350nm-600nm. There may be diodes that emit light signals of 550nm-800nm. Regardless of how many are included, as long as the multiple light-emitting diodes 251 can cover the entire wavelength range, the detection circuit 24 can detect light signals of different wavelength ranges.

[0057] In this way, there is no need to set different light-emitting devices 25 for different wavelengths, which can reduce the complexity of the circuit.

[0058] In some embodiments, such as Figure 5 As shown, the detection circuit 24 may include at least one detector 123. Each detector 123 is connected to the fiber optic head 121 via a corresponding fiber optic cable 122 and is used to receive Gaussian pulse light signals of the corresponding wavelength band and convert the Gaussian pulse light signals of the corresponding wavelength band into corresponding voltage signals. Each detector 123 is also used to determine the corresponding detector state result based on the magnitude of the corresponding voltage signal.

[0059] In embodiments of this application, the detection circuit 24 includes at least one detector 123, such as Figure 5 As shown, at least one detector 123 can be detector A, detector B, detector C, detector D, and detector E. Each detector 123 is connected to an optical fiber head 121 via an optical fiber 122. After receiving a Gaussian pulse light signal, the optical fiber head 121 can reach the detector through the optical fiber 122, so that the detector can obtain the corresponding voltage signal. It should be noted that detectors A, B, C, D, and E can receive light signals of different wavelengths or light signals with overlapping wavelengths, based on the monitoring wavelength of the original detector of the post-light system 12 in the cell analyzer 10.

[0060] In the embodiments of this application, after the front light signal generator 23 emits a Gaussian pulse light signal, each of the at least one detector 123 converts the Gaussian pulse light signal of the corresponding band into a corresponding voltage signal, and then determines the corresponding detector state result based on the magnitude of the corresponding voltage signal.

[0061] For example, if the detection circuit 24 receives a Gaussian pulse signal emitted by the front light signal generator 23, and if the voltage signal detected by detector A is 0, but detectors B, C, D, and E all receive voltage signals, then there is a problem with the branch of detector A where the voltage signal is 0.

[0062] For example, for a light-emitting device including multiple light-emitting diodes 251, since the wavelengths of the light signals emitted by different light-emitting diodes may differ, and the wavelengths of the light signals received by each detector 123 in the detection circuit 24 also differ, the relationship between each light-emitting diode 251 and its corresponding detector 123 can be determined. Accordingly, the controller 21 can also control the state of any one of the multiple light-emitting diodes 251 to perform detection by its corresponding detector 123. The controller 21 can control the multiple light-emitting diodes 251 by setting a switch or other device on each light-emitting diode 251 to achieve control.

[0063] For example, if the M light-emitting diode 251 in the light-emitting device 25 is in the working state, and the other light-emitting diodes 251 are in the off state, and detectors A, B, and C are detectors for their corresponding bands, then the corresponding detection range is the detection branch where detectors A, B, and C are located.

[0064] Thus, for each detector 123, if the voltage signal of the detector is faulty when there is no problem with the input optical signal, it indicates that there is a problem with the corresponding detector branch. Furthermore, by controlling the working state of multiple light-emitting diodes, the corresponding branch of the detector can be detected separately, which can directly locate whether there is a problem in a certain branch, thus improving the efficiency of fault location and providing greater flexibility in detection.

[0065] In some embodiments, the controller 21, connected to the motor driver 22, is configured to control the motor driver 22 to send a Gaussian pulse optical signal emitted by the front optical signal generator 23 into the fiber optic head 121 in response to the detection circuit 24 not receiving an optical signal sent by the front optical system 11, so as to obtain the status monitoring result of the rear optical system 12 based on the magnitude of the voltage signal determined by the detection circuit 24.

[0066] In the embodiments of this application, the controller 21 is connected to the motor driver 22. If the detection circuit 24 does not receive the light signal sent by the front light system 11, that is, each detector 123 in the detection circuit 24 does not detect the corresponding Gaussian pulse light signal, then there may be a problem in the front light system 11. If the controller 21 controls the motor driver 22 to send the Gaussian pulse light signal emitted by the front light signal generator 23 into the fiber optic head 121, and each detector 123 of the detection circuit 24 shows a voltage signal, it indicates that there is a problem with the front light system 11 of the cell analyzer 10, or a problem with the liquid circuit. This indicates that it is unrelated to the rear light system 12, and also indicates that the rear light system 12 is not faulty.

[0067] For example, if at least one detector 123 has no voltage signal before the controller 21 controls the motor driver 22 to send the Gaussian pulse light signal emitted by the front optical signal generator 23 into the fiber optic head 121, that is, when the rear optical system 12 is still receiving the light signal from the front optical system 11, and after the controller 21 controls the motor driver 22 to send the Gaussian pulse light signal emitted by the front optical signal generator 23 into the fiber optic head 121, that is, when the rear optical system 12 receives the Gaussian pulse light signal emitted by the front optical signal generator 23, and at least one detector 123 has a voltage signal, then it indicates that there is a problem with the front optical system 11.

[0068] For example, if, before the controller 21 controls the motor driver 22 to send the Gaussian pulse optical signal emitted by the front optical signal generator 23 into the fiber optic head 121, that is, while the rear optical system 12 is still receiving the optical signal from the front optical system 11, if at least one detector 123 has a voltage signal, it indicates that there is a problem with the rear optical system 12. In this case, there is a problem with the branch where at least one detector 123 does not have a voltage signal.

[0069] In this way, by checking whether the front optical signal generator 23 sends an optical signal to the rear optical system 12, it is possible to directly determine whether the problem is with the front optical system 11 or the rear optical system 12, thus improving the efficiency of troubleshooting.

[0070] In some embodiments, the detector is provided with a photodiode and a calibration bias voltage is provided to fix the gain of the photodiode to a target gain, and the photodiode operates in the linear operating region.

[0071] In the embodiments of this application, a photodiode is used to convert the optical signal transmitted by the optical fiber 122 into a voltage signal. Since the optical signal input and voltage output of the photodiode change exponentially, the conversion of the optical signal into a voltage signal is not linear. Therefore, the photodiode corresponding to each detector 123 can be calibrated to a fixed gain and made to operate in the linear region.

[0072] In the embodiments of this application, if it is required that the detector can detect the light signal, the target gain of the photodiode can be set by the bias voltage of the photodiode. At this time, the controller 21 can be used to control the motor drive circuit to send the light-emitting device 25 of the front light signal generator 23 to the rear light system 12. Then, based on the bias voltage, the voltage signal converted by the expected photodiode is adjusted to the target voltage, and the photodiode is made to work in the linear region.

[0073] In the embodiments of this application, each detector 123 is provided with a photodiode, so that the Gaussian pulse light signal coming from the optical fiber 122 can be converted into a voltage signal.

[0074] In the embodiments of this application, each detector 123 may also be provided with an analog-to-digital converter to convert the analog voltage signal into a digital voltage signal.

[0075] In some embodiments, the front light signal generator 23 includes a temperature control circuit; the temperature control circuit is connected to the light-emitting device 25 and is used to stabilize the output power of the light-emitting device 25.

[0076] In the embodiments of this application, the light-emitting device 25 can be an LED. If the temperature rises, the threshold current of the laser diode will increase and the slope efficiency will decrease, thereby reducing the output power. In addition, the emission wavelength of the light-emitting device 25 is also very sensitive to temperature. This will also affect the Gaussian pulse light signal input to the back light system 12. Therefore, the output power of the light-emitting device 25 can be guaranteed by setting a temperature control circuit.

[0077] For example, the basic working principle of the temperature control circuit can be to monitor the temperature of the light-emitting device 25 in real time by a temperature sensor, compare the measured value with the set value, generate an error signal, and then adjust the control output based on the error signal to drive the cooling element and stabilize the temperature within the set range.

[0078] like Figure 6 As shown, this application provides an exemplary flowchart of a state monitoring method for a rear optical system. The state monitoring system includes a controller, a motor driver, a front optical signal generator, and a detection circuit. The controller is connected to the motor driver; the detection circuit is connected to an optical fiber head via an optical fiber. Exemplarily, the state monitoring method for the rear optical system includes the following steps S601 to S603:

[0079] Step S601: In response to the monitoring command for the rear optical system, the controller controls the motor driver to move the front optical signal generator installed at the output shaft position to the optical path between the front and rear optical systems, and to orient the light-emitting device of the front optical signal generator toward the fiber optic head of the rear optical system.

[0080] In the embodiments of this application, if the cell analyzer is set to perform a self-test upon power-on or to perform quality control upon reaching a preset time, then when the preset time arrives or the cell analyzer is powered on, it indicates that a monitoring command for the rear optical system has been received. The motor driver is then controlled to move the front optical signal generator installed at the output shaft position to the optical path between the front and rear optical systems, blocking the optical signal emitted by the front optical system. Then, the Gaussian pulse signal emitted by the front optical signal generator is output to the fiber optic head of the rear optical system.

[0081] Step S602: The front optical signal generator outputs the same Gaussian pulse optical signal as the front optical system to the fiber head.

[0082] In the embodiments of this application, the front optical signal generator outputs the same Gaussian pulse optical signal as the front optical system to the fiber optic head. In this way, the front optical system and the rear optical system are monitored separately, which avoids the complexity of monitoring the front optical system and the rear optical system together and improves the detection efficiency.

[0083] Step S603: The detection circuit converts the received Gaussian pulse optical signal into a voltage signal, and determines the state monitoring result of the optical system based on the magnitude of the voltage signal.

[0084] In the embodiments of this application, after the detection circuit receives the Gaussian pulse light signal, it can convert the Gaussian pulse light signal into a voltage signal, and then determine the state monitoring result of the optical system based on the magnitude of the voltage signal.

[0085] For example, the detection circuit may include at least one detector. If more than one of the detectors has a voltage signal, it indicates that there is a problem with the other detector branches that do not have a voltage signal. If there is no voltage signal in the detection circuit when the front optical signal generator does not transmit the Gaussian pulse optical signal to the fiber optic head, and there is a voltage signal in the detection circuit when the front optical signal generator transmits the Gaussian pulse signal to the fiber optic head, it indicates that there is a problem with the front optical system and there is no problem with the rear optical system.

[0086] In the embodiments of this application, the detection circuit is equipped with a corresponding analog-to-digital converter for each detector to convert the voltage signal into a digital signal and upload it to the PC software. In this way, the PC software can determine whether there is a signal after each detection, and whether the signal amplitude and coefficient of variation meet the requirements. After the monitoring is completed, the PC software can output a report on the status monitoring results of the back-light system, showing the status of each branch in the status monitoring system, such as whether there is a fault, and roughly where the fault is located.

[0087] This not only allows for the differentiation between the monitoring of the front and rear light systems, but also simplifies the monitoring method and improves monitoring efficiency.

[0088] In some embodiments, the controller is connected to the front light signal generator; the method further includes: the controller acquiring signal characteristic information of the light signal emitted by the microsphere in the front light system, and controlling the front light signal generator to output a Gaussian pulse light signal with the same signal characteristic information.

[0089] In the embodiments of this application, the controller can acquire the signal characteristic information of the light signal emitted by the microsphere in the front light system when both the front and rear light systems are working normally, or at least when the front light system is working normally. In this way, the signal characteristic information of the acquired light signal can be used as a basis for subsequent monitoring. Otherwise, if the signal characteristic information of the acquired light signal itself is problematic, it is impossible to determine whether the problem is in the rear light system.

[0090] In the embodiments of this application, after the controller obtains the signal characteristic information, it will write a program to make the front light signal generator output a Gaussian pulse light signal that is the same as the signal characteristic information.

[0091] This application provides a state monitoring method for a rear optical system, applied to a state monitoring system for a rear optical system. The state monitoring system includes a controller, a motor driver, a front optical signal generator, and a detection circuit. The controller is connected to the motor driver; the detection circuit is connected to the fiber optic connector via an optical fiber. The method includes: the controller, in response to a monitoring command for the rear optical system, controls the motor driver to move the front optical signal generator, mounted on its output shaft, to the optical path between the front and rear optical systems, and aligns the light-emitting device of the front optical signal generator towards the fiber optic connector of the rear optical system; the front optical signal generator outputs a Gaussian pulse optical signal identical to that of the front optical system to the fiber optic connector; the detection circuit converts the received Gaussian pulse optical signal into a voltage signal and determines the state monitoring result of the rear optical system based on the magnitude of the voltage signal. Thus, the rear optical system can be detected using a simulated Gaussian pulse optical signal from the front optical system, separating the front optical system from the monitoring process, reducing the complexity of monitoring, and improving monitoring efficiency.

[0092] It should be understood that the phrase "one embodiment" or "an embodiment" throughout the specification means that a specific feature, structure, or characteristic related to the embodiment is included in at least one embodiment of this application. Therefore, "in one embodiment" or "in an embodiment" appearing throughout the specification does not necessarily refer to the same embodiment. Furthermore, these specific features, structures, or characteristics can be combined in any suitable manner in one or more embodiments. It should be understood that in the various embodiments of this application, the sequence numbers of the above steps / processes do not imply a sequential order of execution; the execution order of each step / process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application. The sequence numbers of the above embodiments of this application are merely descriptive and do not represent the superiority or inferiority of the embodiments.

[0093] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0094] The above description is merely an embodiment of this application, but the scope of protection of this application is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application.

Claims

1. A status monitoring system for a post-optical system, characterized in that, The condition monitoring system includes a controller, a motor driver, a front light signal generator, and a detection circuit. The controller, connected to the motor driver, is used to control the motor driver in response to a monitoring command for the rear optical system, to move the front optical signal generator, which is mounted at the output shaft position, to the optical path between the front optical system and the rear optical system, and to orient the light-emitting device of the front optical signal generator toward the fiber optic head of the rear optical system. The front optical signal generator is used to output the same Gaussian pulse optical signal as the front optical system to the fiber head; The detection circuit is connected to the fiber optic connector via an optical fiber and is used to convert the received Gaussian pulse optical signal into a voltage signal, and to determine the status monitoring result of the back-optical system based on the magnitude of the voltage signal.

2. The status monitoring system for the post-optical system according to claim 1, characterized in that, The controller is connected to the front light signal generator and is also used to acquire the signal characteristic information of the light signal emitted by the microsphere in the front light system, and control the front light signal generator to output the Gaussian pulse light signal that is the same as the signal characteristic information.

3. The status monitoring system for the post-optical system according to claim 2, characterized in that, The front optical signal generator also includes a voltage output circuit and an optical signal driver; The controller is connected to the voltage output circuit and is used to control the voltage output circuit to output a Gaussian pulse voltage signal with the same characteristics as the signal. The optical signal driver is connected to the voltage output circuit and is used to receive the Gaussian pulse voltage signal output by the voltage output circuit and linearly convert the Gaussian pulse voltage signal into a Gaussian pulse current signal. The light-emitting device is connected to the optical signal driver and is used to linearly convert the Gaussian pulse current signal into the Gaussian pulse optical signal output.

4. The status monitoring system for the post-optical system according to claim 2, characterized in that, The light-emitting device includes multiple light-emitting diodes; the light signals emitted by the multiple light-emitting diodes can cover the entire wavelength band.

5. The status monitoring system for the post-optical system according to claim 4, characterized in that, The detection circuit includes at least one detector; Each of the at least one detector is connected to the fiber optic head via a corresponding optical fiber, and is used to receive Gaussian pulse light signals of the corresponding wavelength band and convert the Gaussian pulse light signals of the corresponding wavelength band into corresponding voltage signals. Each detector is also used to determine the corresponding detector state result based on the magnitude of the corresponding voltage signal.

6. The status monitoring system for the post-optical system according to claim 1, characterized in that, The controller, connected to the motor driver, is used to control the motor driver to send the Gaussian pulse optical signal emitted by the front optical signal generator into the optical fiber head in response to the detection circuit not receiving the optical signal sent by the front optical system, so as to obtain the status monitoring result of the rear optical system based on the magnitude of the voltage signal determined by the detection circuit.

7. The status monitoring system for the post-light system according to claim 5, characterized in that, The detector is equipped with a photodiode; The photodiode is provided with a calibrated bias voltage to fix the gain of the photodiode to the target gain, and the photodiode operates in the linear operating region.

8. The status monitoring system for the post-light system according to claim 2, characterized in that, The front optical signal generator includes a temperature control circuit; The temperature control circuit is connected to the light-emitting device and is used to stabilize the output power of the light-emitting device.

9. A method for monitoring the state of a post-optical system, characterized in that, A status monitoring system for a rear-light system includes a controller, a motor driver, a front-light signal generator, and a detection circuit; the controller is connected to the motor driver. The detection circuit is connected to the fiber optic connector via an optical fiber; the method includes: In response to a monitoring command for the rear optical system, the controller controls the motor driver to move the front optical signal generator, which is mounted on the output shaft, to the optical path between the front optical system and the rear optical system, and to orient the light-emitting device of the front optical signal generator toward the fiber optic head of the rear optical system. The front optical signal generator outputs the same Gaussian pulse optical signal as the front optical system to the fiber optic head. The detection circuit converts the received Gaussian pulse light signal into a voltage signal and determines the state monitoring result of the post-light system based on the magnitude of the voltage signal.

10. The method for monitoring the state of a post-optical system according to claim 9, characterized in that, The controller is connected to the front light signal generator; the method further includes: The controller acquires the signal characteristic information of the light signal emitted by the microsphere in the front light system, and controls the front light signal generator to output the Gaussian pulse light signal that is the same as the signal characteristic information.

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