Bacterial-drug co-fermentation preparation for relieving constipation of sows as well as preparation method and application of bacterial-drug co-fermentation preparation

Abstract

The invention discloses a bacterium-medicine co-fermentation preparation for relieving constipation of sows as well as a preparation method and application thereof, relates to the technical field of feeds, and solves the problems that nutrition dilution is possibly caused by single use of high-dose dietary fibers or the effect is slow when single use of traditional Chinese medicines is used, and Cunninghamia lanceolata fibers cannot be directly used for sow feeds. The bacterium and medicine co-fermentation preparation comprises fermentation powder, functional dietary fibers and functional nutrients, and the weight ratio of the fermentation powder to the functional dietary fibers to the functional nutrients is 1: (0.5-2): (0.1-0.3). The bacterium-medicine co-fermentation preparation is good in palatability, can attract sows to eat food, improves the feed intake of the sows, and is also beneficial to improving the intestinal health of the sows and improving the digestion and absorption of digestive tracts of the sows on nutrient substances such as vitamins, mineral substances and fat.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of feed, in particular to a bacteria-drug co-fermentation preparation for relieving constipation of sows, a preparation method and application thereof. BACKGROUND

[0002] Constipation of sows is a common problem in large-scale breeding, and the existing solutions generally have certain limitations: chemical cathartics (such as magnesium sulfate) are easy to cause diarrhea and electrolyte disturbance; the effect of simply supplementing dietary fiber is limited; ordinary probiotics have the problems of low colonization rate and slow effect; traditional Chinese herbal prescriptions mostly rely on ingredients such as rhubarb, mirabilite, hemp seeds or raphanus sativus, which are easy to damage the spleen and stomach due to strong drug properties. In addition, traditional Chinese medicines are mostly prepared by simple crushing and mixing, which has low release rate of effective components, low bioavailability, and is difficult to fully exert the synergistic effect between various medicinal materials. Therefore, it is urgent to develop an efficient, safe, high-bioavailability and formula-innovative comprehensive solution.

[0003] On the other hand, conventional dietary fiber (such as bran, sugar beet meal) has limited water holding capacity and swelling capacity, and may contain anti-nutritional factors. Spruce wood fiber is a high-purity, high-water-holding, high-swelling and chemically inert dietary fiber, which provides a new technical path for solving the problem of sow constipation. However, due to the sensitivity of pregnant and lactating sows to changes in feed palatability, the direct addition of spruce wood fiber will affect the overall palatability of the feed, leading to a decrease in sow feed intake. In addition, spruce wood fiber is a non-fermentable dietary fiber, while what sows need for intestinal health is fermentable dietary fiber to provide substrates for intestinal microorganisms to produce short-chain fatty acids (such as butyric acid). Furthermore, the physical properties of spruce wood fiber will affect the digestion and absorption of nutrients such as vitamins, minerals and fats in the digestive tract.

[0004] In summary, it is a big problem to combine the physical properties of spruce wood fiber with the highly refined nutritional requirements and safety standards of sows, so it has not been successfully applied in sow feed field. SUMMARY

[0005] In view of the above problems, the present application provides a bacteria-drug co-fermentation preparation for relieving constipation of sows, a preparation method and application thereof. The bacteria-drug co-fermentation preparation has good palatability, can attract sows to eat and improve sow feed intake, and is also helpful to improve sow intestinal health, improve the digestion and absorption of nutrients such as vitamins, minerals and fats in the digestive tract of sows, and solve the problems of possible nutrient dilution caused by high-dose dietary fiber alone, slow effect of traditional Chinese medicine alone, and inability of spruce wood fiber to be directly used in sow feed. The specific technical scheme is as follows: A bacteria-drug co-fermentation preparation for relieving constipation of sows, the bacteria-drug co-fermentation preparation comprises fermented powder, functional dietary fiber and functional nutrients, and the weight ratio of the fermented powder, the functional dietary fiber and the functional nutrients is 1:(0.5-2):(0.1-0.3).

[0006] Preferably, the fermented powder comprises the following raw materials in parts by weight: Platycladi Semen 15-25 parts, Pruni Domestici Fructus 10-20 parts, Radix Pseudoginseng 20-30 parts, Radix Paeoniae Alba 10-15 parts, Angelica sinensis 8-12 parts, Platycodon 5-10 parts.

[0007] Preferably, the raw materials of the fermented powder further comprise soybean meal 10-15 parts, brown sugar 1-3 parts, sugar beet meal 10-15 parts and compound fermentation agent 1-5 parts.

[0008] Preferably, the compound fermentation agent is Lactobacillus plantarum (3.9-4.2)×109 CFU / g, Bacillus subtilis (2.4-2.6)×109 CFU / g, Bacillus licheniformis (1.4-1.6)×109 CFU / g, Saccharomyces cerevisiae 1×109 CFU / g and Clostridium butyricum 1×109 CFU / g, and the total number of viable bacteria is ≥1×1010 CFU / g.

[0009] Preferably, the functional dietary fiber is spruce wood fiber powder, and the mesh size is 80-120 mesh.

[0010] Preferably, the functional nutrients comprise the following raw materials in parts by weight: yeast selenium 1-3 parts, yeast zinc 1-3 parts, amino acid 3-7 parts and fatty acid 2-6 parts.

[0011] Preferably, the amino acid is arginine, glutamine and tryptophan in a mass ratio of (1-1.5):1:0.8, and the fatty acid is omega-3 fatty acid.

[0012] Preferably, a preparation method of the bacteria-drug co-fermentation preparation is provided, and the preparation method comprises the following steps: (1) preparing fermented powder: crushing Platycladi Semen, Pruni Domestici Fructus, Radix Pseudoginseng, Radix Paeoniae Alba, Angelica sinensis and Platycodon, and mixing the crushed products with sugar beet meal, soybean meal and brown sugar, sterilizing, inoculating compound fermentation agent, anaerobic fermentation, low-temperature drying and crushing to obtain the fermented powder; (2) mixing: uniformly mixing the fermented powder of step (1) with spruce wood fiber powder and functional nutrients to obtain the bacteria-drug co-fermentation preparation.

[0013] Preferably, in step (1), the crushing is crushing the material to pass through a 50-100 mesh sieve; the sterilization is sterilizing the material by heating to 121 DEG C for 10-15 min; the temperature of the anaerobic fermentation is 37-39 DEG C, and the time is 5-7 days; the low-temperature drying and crushing is drying at 37-39 DEG C for 80-100 min, and then feeding into a crusher to crush to pass through an 80-120 mesh sieve; in step (2), the mixing time is 4-6 min.

[0014] Preferably, the application of the bacteria and medicine co-fermentation preparation in the sow diet is used in the sow diet in the late pregnancy and / or lactation period, and the addition amount is 1%-5% of the weight of the sow diet.

[0015] In the bacteria and medicine co-fermentation preparation, 33.3 kg-66.7 kg of the fermentation powder and 33.3 kg-66.7 kg of the spruce wood fiber powder are contained in 100 kg of the final product.

[0016] Compared with the prior art, the bacteria and medicine co-fermentation preparation has the following beneficial effects: 1. The bacteria and medicine co-fermentation preparation has good palatability, can attract sows to eat, improve the sow feed intake, and help to improve the intestinal health of the sow, improve the digestive tract of the sow to digest and absorb nutrients such as vitamins, minerals and fat, and solve the problem that the physical properties of the spruce wood fiber, which is a good dietary fiber, cannot be directly used in the sow feed.

[0017] 2. The fermentation powder needs a certain time to act on the intestinal flora and the body metabolism, can last for a long time but has a slow effect, and the added spruce wood fiber powder is mild and non-stimulating, can be quickly absorbed after entering the mouth, and can swell after absorbing water, and the unique fiber structure and high water retention (1:8) of the spruce wood fiber powder can gently promote intestinal peristalsis, soften feces, increase the volume and humidity of feces, and provide timely physical defecation effect, that is, the spruce wood fiber powder acts as a physical intestinal peristalsis promoter, has an immediate effect on defecation, the fermentation powder fundamentally regulates the intestinal flora and the body, and the combination of the two can not only quickly regulate the sow intestinal tract, but also can long-term regulate the sow intestinal tract, can not only safely and effectively relieve constipation, but also can completely solve the constipation problem of the sow.

[0018] 3. The spruce wood fiber powder can combine with the prebiotics and probiotics contained in the fermentation powder, provides more abundant and long-acting "food" sources for the probiotics, significantly improves the colonization rate and activity of the probiotics in the intestinal tract, doubles the regulation effect of the mild fermentation powder, avoids the problem of nutrient dilution caused by high-dose dietary fiber or the slow effect caused by single use of traditional Chinese medicine, and realizes more optimal effect at a lower addition amount.

[0019] 4. The bacterial and medicinal co-fermentation preparation of the present application is added to the daily diet of sows, and the improvement rate of constipation symptoms is as high as 93.3% within 3 days, and the cure rate is 100% within 7 days. At the same time, the content of short-chain fatty acids in the intestinal contents of sows is significantly increased by 25%, confirming that the intestinal health is comprehensively improved. DETAILED DESCRIPTION

[0020] The specific embodiments of the present application are described in detail below, but it should be understood that the scope of protection of the present application is not limited by the specific embodiments. Unless otherwise defined, all professional terms used below have the same meaning as generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing the specific embodiments and are not intended to limit the scope of protection of the present application. Unless otherwise specified, the various raw materials, reagents, instruments and equipment used in the present application can be purchased from the market or can be prepared by existing methods.

[0021] Example 1 A bacterial and medicinal co-fermentation preparation for relieving constipation in sows, which is made of fermentation powder, spruce wood fiber powder and functional nutrients in a weight ratio of 1:0.5:0.1, the fermentation powder is made of the following raw materials in parts by weight: Platycladi Cacumen 15 parts, Pruni Mume Fructus 10 parts, Radix Pseudoginseng 20 parts, Radix Astragali 10 parts, Radix Rehmanniae 10 parts, Angelica sinensis 8 parts, Platycodon Grandiflorum 5 parts, Soybean Meal 10 parts, Brown Sugar 1 part, Sugar Beet Meal 10 parts, Compound Fermentation Inoculum 1 part; the functional nutrients are made of the following raw materials in parts by weight: Yeast Selenium 1 part, Yeast Zinc 1 part, Amino Acid 3 parts, Omega-3 Fatty Acid 2 parts; the amino acid is arginine, glutamine and tryptophan in a mass ratio of 1:1:0.8.

[0022] The compound fermentation inoculum is 3.9×10 9 CFU / g of Lactobacillus plantarum, 2.6×10 9 CFU / g of Bacillus subtilis, 1.5×10 9 CFU / g of Bacillus licheniformis, 1×10 9 CFU / g of Saccharomyces cerevisiae, and 1×10 9 CFU / g of Clostridium butyricum.

[0023] A preparation method of the bacterial and medicinal co-fermentation preparation as described above, comprising the following steps: (1) Preparation of fermentation powder: grind Platycladi Cacumen, Pruni Mume Fructus, Radix Pseudoginseng, Radix Astragali, Radix Rehmanniae, Angelica sinensis and Platycodon Grandiflorum to pass through a 50-mesh sieve, mix with sugar beet meal, soybean meal and brown sugar, heat the material to 121℃ and sterilize for 10 min, inoculate the compound fermentation inoculum, anaerobically ferment at 37℃ for 5 days, dry at 37℃ for 80 min, then grind to pass through an 80-mesh sieve in a pulverizer to obtain the fermentation powder; (2) Mixing: the fermented powder material of step (1) is mixed with spruce wood fiber powder with a particle size of 80 mesh and functional nutrients for 4 min to obtain the bacteria-medicine co-fermented preparation.

[0024] The bacteria-medicine co-fermented preparation is used in the diet of sows in the late pregnancy and / or lactation period, and the addition amount is 5% of the weight of the sow diet.

[0025] Example 2 A bacteria-medicine co-fermented preparation for relieving constipation of sows is prepared from fermented powder material, spruce wood fiber powder and functional nutrients in a weight ratio of 1:2:0.3. The fermented powder material is prepared from the following raw materials in parts by weight: Platycladi Cacumen 25 parts, Pruni Mume Fructus 20 parts, Radix Pseudostellariae 30 parts, Radix Astragali 15 parts, Radix Rehmanniae 15 parts, Angelica sinensis 12 parts, Platycodonis 10 parts, soybean meal 15 parts, brown sugar 3 parts, sugar beet meal 15 parts, and compound fermentation agent 5 parts. The functional nutrients are prepared from the following raw materials in parts by weight: selenium yeast 3 parts, zinc yeast 3 parts, amino acid 7 parts, and omega-3 fatty acid 6 parts. The amino acid is arginine, glutamine and tryptophan in a mass ratio of 1.5:1:0.8.

[0026] The compound fermentation agent is 4.2×10 9 CFU / g of Lactobacillus plantarum, 2.4×10 9 CFU / g of Bacillus subtilis, 1.5×10 9 CFU / g of Bacillus licheniformis, 1×10 9 CFU / g of Saccharomyces cerevisiae, and 1×10 9 CFU / g of Clostridium butyricum.

[0027] A preparation method of the bacteria-medicine co-fermented preparation, comprising the following steps: (1) Preparation of fermented powder material: Platycladi Cacumen, Pruni Mume Fructus, Radix Pseudostellariae, Radix Astragali, Radix Rehmanniae, Angelica sinensis and Platycodonis are crushed to pass through a 100-mesh sieve, mixed with sugar beet meal, soybean meal and brown sugar, and then the material is heated to 121℃ and sterilized for 15 min. The compound fermentation agent is inoculated and anaerobically fermented at 39℃ for 7 days. Then it is dried at 39℃ for 100 min, and then sent to a pulverizer to be crushed to pass through a 120-mesh sieve to obtain the fermented powder material; (2) Mixing: the fermented powder material of step (1) is mixed with spruce wood fiber powder with a particle size of 120 mesh and functional nutrients for 6 min to obtain the bacteria-medicine co-fermented preparation.

[0028] The bacteria-medicine co-fermented preparation is used in the diet of sows in the late pregnancy and / or lactation period, and the addition amount is 5% of the weight of the sow diet.

[0029] Example 3 A bacteria-drug co-fermentation preparation for relieving constipation of sows, which is made of fermentation powder, spruce wood fiber powder and functional nutrients in a weight ratio of 1:1:0.15, the fermentation powder is made of raw materials in the following weight parts: Platycladi Cacumen 17 parts, Pruni 12 parts, Radix Pseudostellariae 22 parts, Radix Astragali 11 parts, Radix Rehmanniae 11 parts, Angelica sinensis 9 parts, Platycodonis 6 parts, soybean meal 11 parts, brown sugar 1.5 parts, sugar beet meal 11 parts, and compound fermentation agent 2 parts; the functional nutrients are made of raw materials in the following weight parts: yeast selenium 1.5 parts, yeast zinc 1.5 parts, amino acid 4 parts, and omega-3 fatty acid 3 parts; the amino acid is arginine, glutamine and tryptophan in a mass ratio of 1.2:1:0.8.

[0030] The compound fermentation agent is 4.0×10 9 CFU / g of Lactobacillus plantarum, 2.5×10 9 CFU / g of Bacillus subtilis, 1.6×10 9 CFU / g of Bacillus licheniformis, 1×10 9 CFU / g of Saccharomyces cerevisiae, and 1×10 9 CFU / g of Clostridium butyricum.

[0031] A preparation method of the bacteria-drug co-fermentation preparation, comprising the following steps: (1) preparing fermentation powder: crushing Platycladi Cacumen, Pruni, Radix Pseudostellariae, Radix Astragali, Radix Rehmanniae, Angelica sinensis and Platycodonis to pass through a 60-mesh sieve, mixing with sugar beet meal, soybean meal and brown sugar, sterilizing the materials by heating to 121℃ and keeping for 11 min, inoculating the compound fermentation agent, anaerobically fermenting at 37℃ for 5.5 days, drying at 37℃ for 85 min, and then crushing to pass through a 90-mesh sieve to obtain the fermentation powder; (2) mixing: mixing the fermentation powder of step (1) with spruce wood fiber powder having a particle size of 90 mesh and functional nutrients for 4.5 min to obtain the bacteria-drug co-fermentation preparation.

[0032] The bacteria-drug co-fermentation preparation is used in the diet of sows in the late pregnancy and / or lactation period, and the addition amount is 2% of the weight of the diet of sows.

[0033] Example 4 A kind of bacteria medicine co-fermentation preparation for relieving sow constipation, the bacteria medicine co-fermentation preparation is made of weight ratio 1:1.5:0.25 of fermentation powder, spruce wood fiber powder, functional nutrient, fermentation powder is made of the following weight parts of raw materials: Platycladi Seed 22 parts, Prunus Domestica 18 parts, raw atractylodes 28 parts, Radix Angelicae Sinensis 11 parts, Anemarrhena 9 parts, soybean meal 14 parts, brown sugar 2.5 parts, sugar beet meal 14 parts, compound fermentation inoculant 4 parts;Functional nutrient is made of the following weight parts of raw materials: Yeast Selenium 2.5 parts, Yeast Zinc 2.5 parts, Amino Acid 6 parts, ω-3 fatty acid 5 parts;Amino acid is mass ratio 1.4:1:0.8 of arginine, glutamine, tryptophan.

[0034] Compound fermentation inoculant is 4.1 × 10 9 CFU / g of Lactobacillus plantarum, 2.6 × 10 9 CFU / g of Bacillus subtilis, 1.5 × 10 9 CFU / g of Bacillus licheniformis, 1 × 10 9 CFU / g of Saccharomyces cerevisiae, 1 × 10 9 CFU / g of Clostridium butyricum.

[0035] A kind of preparation method of the above-mentioned bacteria medicine co-fermentation preparation, comprising the following steps: (1) preparation of fermentation powder: Platycladi Seed, Prunus Domestica, raw atractylodes, Radix Angelicae Sinensis, Anemarrhena, are ground to pass 90 mesh sieve, mixed with sugar beet meal, soybean meal, brown sugar, the material is heated to 121 DEG C and sterilized for 14 min, inoculated with compound fermentation inoculant, anaerobic fermentation at 39 DEG C temperature for 6.5 d, dried at 39 DEG C temperature for 95 min, then sent into the pulverizer and ground to pass 110 mesh sieve, to obtain fermentation powder; (2) mixing: the fermentation powder of step (1) is mixed with 110 mesh spruce wood fiber powder and functional nutrient for 5.5 min, to obtain the bacteria medicine co-fermentation preparation.

[0036] A kind of bacteria medicine co-fermentation preparation in the above-mentioned application in sow diet, is used in sow diet in late pregnancy and / or lactation, and the addition amount is 4% of the weight of sow diet.

[0037] Example 5 A kind of bacteria medicine co-fermentation preparation for relieving sow constipation, the bacteria medicine co-fermentation preparation is made of fermentation powder, spruce wood fiber powder, functional nutrient with weight ratio of 1:1.3:0.2, fermentation powder is made of the following raw materials by weight parts: Platycladi Cacumen 20 parts, Pruni 15 parts, raw Atractylodes 25 parts, Radix Angelicae Sinensis 10 parts, 8 parts of Platycodon, 13 parts of soybean meal, 2 parts of brown sugar, 10-15 parts of sugar beet meal, 3 parts of compound fermentation agent;Functional nutrient is made of the following raw materials by weight parts: Yeast Selenium 2 parts, Yeast Zinc 2 parts, Amino Acid 5 parts, Omega-3 fatty acid 4 parts;Amino acid is arginine, glutamine, tryptophan with mass ratio of 1.3:1:0.8.

[0038] Compound fermentation agent is 4 × 10 9 CFU / g of Lactobacillus plantarum, 2.5 × 10 9 CFU / g of Bacillus subtilis, 1.6 × 10 9 CFU / g of Bacillus licheniformis, 1 × 10 9 CFU / g of Saccharomyces cerevisiae, 1 × 10 9 CFU / g of Clostridium butyricum.

[0039] A kind of preparation method of bacteria medicine co-fermentation preparation as described above, comprising the following steps: (1) preparation of fermentation powder: Platycladi Cacumen, Pruni, raw Atractylodes, Radix Angelicae Sinensis, Platycodon are crushed to pass 70 mesh sieve, mixed with sugar beet meal, soybean meal and brown sugar, the material is heated to 121 DEG C and sterilized for 13 min, inoculated with compound fermentation agent, anaerobic fermentation at 38 DEG C for 6d, dried at 38 DEG C for 90 min, then sent into the pulverizer and crushed to pass 100 mesh sieve, to obtain fermentation powder; (2) mixing: the fermentation powder of step (1) is mixed with spruce wood fiber powder with 100 mesh particle size and functional nutrient for 5 min to obtain the bacteria medicine co-fermentation preparation.

[0040] A kind of bacteria medicine co-fermentation preparation as described above is applied in sow diet, is used in sow diet in late pregnancy and / or lactation, and the addition amount is 3% of the weight of sow diet.

[0041] Example 6 Different from example 3 is that no sugar beet meal is added in the raw materials of fermentation powder, and other conditions are unchanged.

[0042] Example 7 Different from example 3 is that the fermentation strain is compound fermentation agent, and the amount of Lactobacillus plantarum is equal, and other conditions are unchanged.

[0043] Example 8 The difference from Example 3 is that no functional dietary fiber is added, and functional dietary fiber (spruce wood fiber powder) is added to the raw material of the fermented powder in an equal amount of konjac powder, and other conditions are unchanged.

[0044] Example 9 The difference from Example 3 is that no functional nutrient is added, and other conditions are unchanged.

[0045] Application Test 1 120 pregnant sows similar in body shape, body weight, and constipation symptoms and frequency were selected and randomly divided into 6 groups, 20 sows in each group, and the sows in each group were fed with the following feed: Example 3 group: basic daily ration + 2% basic daily ration weight of the bacteria and medicine co-fermented preparation prepared in Example 3; Example 6 group: basic daily ration + 2% basic daily ration weight of the bacteria and medicine co-fermented preparation prepared in Example 6; Example 7 group: basic daily ration + 2% basic daily ration weight of the bacteria and medicine co-fermented preparation prepared in Example 7; Example 8 group: basic daily ration + 2% basic daily ration weight of the bacteria and medicine co-fermented preparation prepared in Example 8; Example 9 group: basic daily ration + 2% basic daily ration weight of the bacteria and medicine co-fermented preparation prepared in Example 9.

[0046] Control group: basic daily ration + 2% basic daily ration weight of pure water.

[0047] The basic daily ration is prepared from the following raw materials: 622 kg of corn, 100 kg of flour, 20 kg of soybean oil, 180 kg of soybean meal, 30 kg of fish meal, 15 kg of stone powder, 15 kg of calcium hydrogen phosphate, 3 kg of L-lysine hydrochloride, 2 kg of DL-methionine, 3 kg of L-threonine, 5 kg of sodium chloride, 3 kg of compound vitamin, and 2 kg of compound trace element. The preparation method is as follows: the corn, flour, soybean oil, soybean meal, fish meal, stone powder, calcium hydrogen phosphate, L-lysine hydrochloride, and sodium chloride are mixed uniformly, the material is heated to 121℃ and sterilized for 15 min, 8x10 9 CFU / g of Lactobacillus plantarum is inoculated, anaerobic fermentation is carried out at 38℃ for 7 d, drying is carried out at 39℃ for 100 min, then the material is crushed to pass through a 100 mesh sieve, DL-methionine, L-threonine, compound vitamin, and compound trace element are added, and stirring is carried out uniformly to obtain the basic daily ration.

[0048] Moreover, each kg of the basic daily ration contains 18000 IU of vitamin A, 2000 IU of vitamin D3, 50 mg of vitamin E, 2 mg of vitamin K, 0.8 mg of vitamin B1, 6.5 mg of vitamin B2, 4 mg of vitamin B6, 0.02 mg of vitamin B12, 18.5 mg of niacin, 15.5 mg of pantothenic acid, 1 mg of folic acid; each kg of the basic daily ration contains 160 mg of iron, 15 mg of copper, 100 mg of zinc, 40 mg of manganese, 0.1 mg of selenium, and 0.5 mg of iodine.

[0049] The test period is 7 days, and the initial number of test pigs, the final number of test pigs, the incidence of constipation (%), the average feces score (score standard: 1 point = dry and hard, 2 points = solid, 3 points = normal stool without compaction signs, 4 points = loose stool, and 5 points = watery), the average daily feed intake (kg / d), the total SCFA in feces (mmol / kg), and the number of lactic acid bacteria in feces (log10 CFU / g) are recorded during the experiment. The experimental results are shown in Table 1.

[0050] Table 1 Note: The data are represented as mean ± standard deviation. P<0.05 indicates a significant difference, and P<0.01 indicates a very significant difference.

[0051] The fungus and medicine co-fermented preparation containing spruce wood fiber provided by the present application can significantly reduce the incidence of constipation in late pregnant sows, effectively improve the feces form of sows, and optimize the intestinal flora structure, greatly increase the intestinal SCFA level and the number of beneficial bacteria, thereby comprehensively improving the intestinal health of sows.

[0052] Application Test 2 30 late pregnant sows with similar body type, body weight, and constipation symptoms and frequency are selected, and the fungus and medicine co-fermented preparation prepared in Example 1 is fed to the sows at 2% of the weight of the basic daily ration every day. The basic daily ration used is the same as that disclosed in Application Test 1, the test period is 7 days, and the number of constipated sows on the 0th day, the 3rd day, and the 7th day and the short-chain fatty acid content in the intestinal contents of the sows are recorded during the experiment. The experimental results are shown in Table 2.

[0053] Table 2 Note: The constipated sows show scattered granular feces.

[0054] From the number of experiments in Table 2, it can be seen that the bacteria and medicine co-fermentation preparation of the application is added in the sow diet, and the improvement rate of constipation symptoms within 3 days is as high as 93.3%, and the cure rate within 7 days is 100%. At the same time, the content of short-chain fatty acids in the intestinal contents of sows is significantly increased by 25%, which confirms that the intestinal health is comprehensively improved. The spruce wood fiber powder of the application can combine with the prebiotics and probiotics contained in the fermented powder material, providing more abundant and long-acting "food" source for probiotics, significantly improving the colonization rate and activity of probiotics in the intestinal tract, doubling the conditioning effect of the fermented powder material with mild drug properties, avoiding the problem of nutrient dilution that may be caused by using high-dose dietary fiber alone or the slow effect of using traditional Chinese medicine alone, and achieving better results at a lower addition amount. The intestinal health of sows is comprehensively improved by adding the bacteria and medicine co-fermentation preparation of the application in the sow diet.

[0055] The foregoing description of specific exemplary embodiments of the application is intended to be illustrative only and is not intended to limit the application to the precise forms described. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the application. The exemplary embodiments are chosen and described in order to explain the principles of the application and its practical application and to allow others skilled in the art to understand the application for various exemplary embodiments with various modifications being applicable. The scope of the application is intended to be defined by the claims and their equivalents.

Claims

1. A bacterio-phyto co-fermented preparation for use in alleviating constipation in sows, characterized in that, The bacteria-medicine co-fermentation preparation comprises fermented powder, functional dietary fiber and functional nutrient, and the weight ratio of the fermented powder, the functional dietary fiber and the functional nutrient is 1:(0.5-2):(0.1-0.3).

2. The fungicide-fermenting agent for use according to claim 1, characterized in that, The fermented powder comprises the following raw materials in parts by weight: Platycladi Cacumen 15-25 parts, Pruni Domestici Fructus 10-20 parts, Radix Pseudoginseng 20-30 parts, Radix Paeoniae Alba 10-15 parts, Angelica sinensis 8-12 parts and Platycodon 5-10 parts.

3. The fungicide-fermenting agent for use according to claim 2, characterized in that, The raw materials of the fermented powder further comprise soybean meal 10-15 parts, brown sugar 1-3 parts, sugar beet meal 10-15 parts and compound fermentation agent 1-5 parts.

4. The fungicide-fermenting agent for use according to claim 3, characterized in that, The complex fermentation microbial agent is (3.9-4.2) x 10 9 CFU / g of Lactobacillus plantarum, (2.4-2.6) x 10 9 CFU / g of Bacillus subtilis, (1.4-1.6) x 10 9 CFU / g of Bacillus licheniformis, 1 x 10 9 CFU / g of Saccharomyces cerevisiae, 1 x 10 9 CFU / g of Clostridium butyricum, and the total viable bacteria number is ≥ 1 x 10 10 CFU / g.

5. The fungicide-fermenting agent for use according to claim 1, characterized in that, The functional dietary fiber is spruce wood fiber powder, and the mesh size is 80-120 mesh.

6. The fungicide-fermenting agent for use in claim 1, characterized in that, The functional nutrient comprises the following raw materials in parts by weight: yeast selenium 1-3 parts, yeast zinc 1-3 parts, amino acid 3-7 parts and fatty acid 2-6 parts.

7. The fungicide-fermenting agent for use according to claim 6, characterized in that, The amino acid is arginine, glutamine and tryptophan in a mass ratio of (1-1.5):1:0.8, and the fatty acid is omega-3 fatty acid.

8. A method for preparing a bacterio-phytoco ferment preparation according to any one of claims 1 to 7, characterized by, The method comprises the following steps: (1) preparing fermented powder: crushing Platycladi Cacumen, Pruni Domestici Fructus, Radix Pseudoginseng, Radix Paeoniae Alba, Angelica sinensis and Platycodon, mixing with sugar beet meal, soybean meal and brown sugar, sterilizing, inoculating compound fermentation agent, anaerobic fermentation, low-temperature drying and crushing to obtain fermented powder; (2) mixing: uniformly mixing the fermented powder of step (1) with spruce wood fiber powder and functional nutrient to obtain the bacteria-medicine co-fermentation preparation.

9. The preparation method according to claim 8, characterized in that, In step (1), the crushing is crushing the materials to pass through a 50-100 mesh sieve; the sterilization is sterilizing the materials by heating to 121 ℃ for 10-15 min; the temperature of the anaerobic fermentation is 37-39 ℃, and the time is 5-7 days; the low-temperature drying and crushing is drying at 37-39 ℃ for 80-100 min, and then crushing to pass through an 80-120 mesh sieve; in step (2), the mixing time is 4-6 min.

10. The use of a bacteriophage co-fermented preparation according to any one of claims 1 to 7 in the daily ration of sows, characterized in that, The bacteria-medicine co-fermentation preparation is used in the daily ration of sows in the late gestation period and / or lactation period, and the addition amount is 1%-5% of the weight of the daily ration of sows.

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