Composition for improving permeation effect of bark extract of Campanumoea maculata and preparation method and application of composition

Abstract

The invention provides a composition for improving the permeation effect of a Campania maculata bark extract as well as a preparation method and application of the composition, and relates to the technical field of cosmetics. The invention provides an anti-aging composition for improving the permeation effect of a Campandra maculata bark extract. The anti-aging composition comprises recombinant human III type collagen, the Campandra maculata bark extract, an annona squamosa fruit extract, a Flos Magnoliae extract and a film-forming agent, the film-forming agent is composed of rhamnose and a trimethyl pentanediol / adipic acid / glycerol cross-linked polymer. The combination of the recombinant human III type collagen and the film-forming agent can strengthen hydration of cuticle, optimize the controlled release efficiency of active ingredients and broaden the permeation path, thereby forming an efficient permeation network from the skin surface to the deep layer. The combination of the Campandra mollissima bark extract, the Annona mollissima fruit extract and the Flos Magnoliae extract can construct a multi-dimensional antioxidant network, activate longevity proteins to resist saccharification and DNA damage, and comprehensively delay skin aging.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a composition for improving the penetration effect of Eupatorium Fortunei extract, a preparation method and application thereof. BACKGROUND

[0002] With the in-depth study of skin aging biology, skin aging is confirmed to be a complex process involving both internal mechanisms and external environment, which is manifested in a series of problems such as wrinkle formation, skin relaxation, decreased elasticity, impaired barrier function, and decreased gloss. In view of skin aging, the research focus in the field of cosmetics has shifted from traditional surface covering and basic moisturizing to achieving anti-aging and repair by adding high-activity ingredients (such as plant extracts, polypeptides, vitamins, etc.).

[0003] However, the stratum corneum, the outermost layer of the skin, constitutes a natural physical barrier that protects the body from external stimuli and prevents water loss, while also greatly limiting the transdermal absorption of active ingredients. Most active substances with a molecular weight greater than 500 Da or hydrophilicity have difficulty effectively penetrating this "brick wall structure", resulting in extremely low bioavailability and an inability to achieve an effective concentration in the target skin layer (such as the dermis), thereby severely restricting the efficacy of the final product.

[0004] Currently, in order to improve the penetration of active substances, chemical penetration enhancers, physical penetration enhancement techniques, and carrier systems are commonly used. Among them, chemical penetration enhancers include azone, alcohols, fatty acids, etc. Although these substances can disrupt the lipid structure of the stratum corneum to increase penetration, they often have potential skin irritation, and long-term use can damage the skin barrier, causing sensitivity, inflammation, and other problems, making it difficult to balance safety and efficacy; physical penetration enhancement techniques, such as micro-needles, ultrasound induction, etc., have significant effects, but are complex to operate, costly, and usually require professional personnel to operate in professional institutions, making it difficult to apply to daily household skincare products; carrier systems, such as liposomes, nanoemulsions, polymer microparticles, etc., can encapsulate active substances to assist their penetration through the stratum corneum. However, existing carrier systems have complex preparation processes or high costs. In addition, traditional penetration enhancers often have single functions, and there is an urgent need for a new solution that not only safely and efficiently breaks through the skin barrier to deliver multiple active ingredients, but also has certain skincare functions itself, thereby achieving the perfect integration of penetration enhancement and efficacy.

[0005] Therefore, it is of great significance to develop a new type of multifunctional composition that improves the transdermal penetration rate of multiple anti-aging ingredients without damaging the skin, for the development of the next generation of high-performance skincare products. SUMMARY

[0006] The present application aims to provide a composition for improving the permeation effect of Eucalyptus punctata tree bark extract and a preparation method and application thereof, and the composition provided by the present application has significant anti-aging effect.

[0007] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions. The present application provides an anti-aging composition for improving the permeation effect of Eucalyptus punctata tree bark extract, which comprises the following raw materials by weight: 0.05-0.2 parts of recombinant human type III collagen, 0.05-0.1 parts of Eucalyptus punctata tree bark extract, 0.01-0.03 parts of annona glabra fruit extract, 0.03-0.08 parts of jasminum mesnyi flower extract, and 1.5-2.5 parts of a film-forming agent; the film-forming agent is composed of rhamnose and trimethylpentanediol / adipic acid / glycerin cross-linked polymer.

[0008] Preferably, the weight ratio of the rhamnose and trimethylpentanediol / adipic acid / glycerin cross-linked polymer is (0.5-1):(1-1.5).

[0009] Preferably, the annona glabra fruit extract is obtained by anaerobic fermentation of annona glabra fruit with complex microorganisms.

[0010] More preferably, the complex microorganisms are composed of lactobacillus plantarum WCFS1 and animal bifidobacterium W3.

[0011] Preferably, the jasminum mesnyi flower extract is obtained by extraction of jasminum mesnyi flower buds with a deep eutectic solvent and purification with a macroporous adsorption resin.

[0012] More preferably, the deep eutectic solvent is composed of choline chloride and glycerol.

[0013] The present application also provides a preparation method of the above-mentioned anti-aging composition, which comprises: mixing recombinant human type III collagen, Eucalyptus punctata tree bark extract, jasminum mesnyi flower extract, annona glabra fruit extract, rhamnose, trimethylpentanediol / adipic acid / glycerin cross-linked polymer, and water, homogenizing to obtain the anti-aging composition.

[0014] Preferably, the rotation speed of the homogenization is 3000-4000 rpm, and the time is 10-15 min.

[0015] The present application also provides an application of the above-mentioned composition in the preparation of anti-aging and repair products.

[0016] Preferably, the aging is photoaging and natural aging.

[0017] Compared with the prior art, the present application has the following beneficial effects: The application provides an anti-aging composition for improving the penetration effect of Erythrina variegate tree bark extract, which comprises recombinant human type III collagen, Erythrina variegate tree bark extract, annona glabra fruit extract, lantana camara flower extract and a film-forming agent; the film-forming agent is composed of rhamnose and trimethylpentanediol / adipic acid / glycerin cross-linked polymer. The recombinant human type III collagen is used in combination with the film-forming agent to strengthen the hydration of the stratum corneum, optimize the controlled release efficiency of active ingredients and broaden the penetration path, forming an efficient penetration network from the skin surface to the deep layer. The combination of Erythrina variegate tree bark extract, annona glabra fruit extract and lantana camara flower extract can construct a multi-dimensional antioxidant network, protect the support structure from promoting collagen synthesis and inhibiting degradation, activate long-lived proteins to resist glycation and DNA damage, and comprehensively delay skin aging (wrinkles, slackening and dullness); through multi-target anti-inflammatory soothing sensitive, the barrier is reconstructed from the stratum corneum to the deep cell structure, supporting wound healing and improving tolerance, and it is suitable for sensitive skin, damaged skin and post-medical art care. The composition of the application can be widely applied in the field of skin care products or anti-aging drugs by multi-component compounding to enhance the transdermal efficiency and anti-aging effect of active substances, and achieve better skin repair and youth effect. DETAILED DESCRIPTION

[0018] The application provides an anti-aging composition for improving the penetration effect of Erythrina variegate tree bark extract, which comprises the following raw materials in parts by weight: 0.05-0.2 parts of recombinant human type III collagen, 0.05-0.1 parts of Erythrina variegate tree bark extract, 0.01-0.03 parts of annona glabra fruit extract, 0.03-0.08 parts of lantana camara flower extract and 1.5-2.5 parts of a film-forming agent; the film-forming agent is composed of rhamnose and trimethylpentanediol / adipic acid / glycerin cross-linked polymer.

[0019] Compared with traditional chemical penetration enhancers (such as alcohol and propylene glycol), the recombinant human type III collagen of the application is more mild in penetration promotion, can combine with keratin in the stratum corneum through hydrogen bonds, adsorb water molecules to form a hydrated microenvironment, broaden the penetration channel, and can also encapsulate Erythrina variegate tree bark extract to target delivery to the dermis without damaging the lipid structure of the stratum corneum. In addition to the penetration promotion effect, the recombinant human type III collagen has high homology with human collagen and no allergenicity, can promote the proliferation and differentiation of keratinocytes, and cooperate with annona glabra fruit extract and lantana camara flower extract to repair the skin barrier; it can also supplement type III collagen in the dermis, form synergy with Erythrina variegate tree bark extract in inhibiting collagen degradation, strengthen the anti-aging effect, adapt to sensitive skin and post-medical art care, and avoid the dryness and irritation problems of traditional chemical penetration enhancers.

[0020] The weight ratio of rhamnose and trimethylpentanediol / adipic acid / glycerin cross-linked polymer in the application is preferably (0.5-1):(1-1.5), more preferably 0.8:1.2.

[0021] Rhamnose can enhance the moisturizing ability of the skin barrier by film-forming and moisturizing, and can form a breathable film on the surface of the skin. This film not only reduces water evaporation, but also increases the residence time of other active ingredients (such as Euphorbia tithymaloides extract) on the surface of the skin, thereby promoting their gradual penetration. Rhamnose also has soothing properties, which can reduce the impact of external stimuli on the skin, creating a more stable environment for active ingredient penetration. The trimethylpentanediol / adipic acid / glycerin crosslinked polymer has excellent film-forming and stability, and the film formed has the characteristics of balance between breathability and flexibility, which can wrap fat-soluble or water-soluble active ingredients, has a controlled release effect, and enhances the adhesion of active ingredients on the skin surface, reduces the loss of active ingredients due to volatilization or washing, and indirectly improves the penetration efficiency. The film-forming agent of the present application can form a breathable film on the skin surface, maintain hydration of the stratum corneum by locking water, soften the skin structure to promote active ingredient penetration, and its adhesion can prolong the residence time of active substances, achieve sustained release and absorption, thereby synergistically enhancing the penetration effect of Euphorbia tithymaloides extract and other components.

[0022] The recombinant human type III collagen and the film-forming agent have a synergistic effect in promoting penetration: the recombinant human type III collagen itself has good skin affinity and moisturizing properties, which can soften the stratum corneum and create favorable conditions for the penetration of other active ingredients. When the film-forming agent (rhamnose and trimethylpentanediol / adipic acid / glycerin crosslinked polymer) forms a film on the skin surface, it not only locks in moisture and maintains a high hydration state, but also prolongs the contact time of active substances with the skin through a slow-release effect. The collagen and the film-forming agent work together to further enhance the permeability of the stratum corneum, thereby significantly improving the penetration efficiency and bioavailability of active ingredients such as Euphorbia tithymaloides extract.

[0023] The Euphorbia tithymaloides extract of the present application is preferably the Euphorbia tithymaloides extract 1 in CN119837808B. The preparation method of the Euphorbia tithymaloides extract 1 is as follows: S1. Dry the Euphorbia tithymaloides bark to a water content of ≤5%, and grind it into coarse powder; S2. Mix the Euphorbia tithymaloides coarse powder with rapeseed oil and polyglyceryl-3 diisostearate, heat to 55℃, and stir at 300rpm for 20min to obtain a mixed solution; the mass ratio of Euphorbia tithymaloides coarse powder, rapeseed oil and polyglyceryl-3 diisostearate is 1:3:0.2; S3. Under N2 atmosphere, ultrasonic-assisted extraction is performed on the mixed solution in S2 to obtain an extract, the extraction temperature is 55℃, the ultrasonic frequency is 30kHz, and the extraction time is 45min; S4. Filter the extract to remove insoluble matter, centrifuge, and take the supernatant to obtain the Euphorbia tithymaloides extract.

[0024] The extract of the tree bark of Planchonella macropora contains polyphenols (such as flavonoids, tannins), polyols and terpenoids, can directly scavenge free radicals (ROS, hydroxyl radicals), inhibit lipid peroxidation reaction, provide electrons to neutralize oxidative active molecules, inhibit collagen degradation; and can activate Sirtuins family proteins to realize DNA repair, anti-glycation, telomere maintenance and inflammation regulation, so as to achieve anti-aging effect; and has the repairing effects of repairing deep cells, strengthening skin barrier and relieving inflammation. The extract of the tree bark of Planchonella macropora can be applied to skin care products to achieve the overall effects of lightening wrinkles, tightening skin, improving dullness, enhancing skin toughness and gloss.

[0025] The Annona glabra fruit extract is preferably obtained by anaerobic fermentation of Annona glabra fruit by a composite microorganism, and the composite microorganism preferably consists of Lactobacillus plantarum WCFS1 and Bifidobacterium animalis W3. 8 The number of bacteria in the composite microorganism is preferably (6-10) x 10 8 The number of bacteria in the composite microorganism is preferably (6-10) x 10 8 The number of bacteria in the composite microorganism is preferably (6-10) x 10 8 The inoculation amount of the composite microorganism is preferably 3%-5% of the weight of the slurry, and more preferably 4%. The preparation method of the Annona glabra fruit extract preferably comprises: after intermittent crushing mode of a cell disrupter is operated at 20000-30000 rpm for 30 s, the operation is paused for 30 s, and the operation is repeated for 3-4 times, a slurry is obtained; 12-17 times the weight of the slurry is added with water, and after sterilization, the composite microorganism is inoculated; anaerobic fermentation is carried out at 35-40 DEG C and pH 6-7 for 56-72 h; ultraviolet lamp sterilization is carried out; 0.22 mu m membrane filtration is carried out; the filtrate is vacuum freeze-dried to obtain the Annona glabra fruit extract.

[0026] The Lactobacillus plantarum WCFS1 and the Bifidobacterium animalis W3 are compounded to ferment the Chrysophyllum coccineum, ingredients in the Chrysophyllum coccineum are decomposed and transformed, the types of active ingredients in the extract are enriched, and the content of active ingredients (such as fermented polyphenols, short-chain fatty acids and active peptides) in the extract is improved. The preparation of the Chrysophyllum coccineum extract realizes efficient retention of active ingredients and functional synergism, delays aging through antioxidation, promotes collagen synthesis and moisturizing and anti-wrinkle, supports skin recovery through anti-inflammatory soothing, barrier reconstruction and wound healing in repair, has dual values of anti-aging and repair, is suitable for skin care products, medical dressings and the like, and is especially suitable for nursing of young-old skin, sensitive skin and medical beauty postoperative.

[0027] The preparation method of the Jasminum mesnyi flower extract preferably comprises: crushing dry Jasminum mesnyi flower buds through a 50-100 mesh sieve to obtain Jasminum mesnyi powder, adding a eutectic solvent aqueous solution at 1:15-20 g / mL, extracting at 60-70 ℃ and 200-400 rpm for 60-90 min, cooling to 25-30 ℃, and centrifuging at 8000-10000 rpm for 10-15 min to obtain supernatant; adsorbing the supernatant through an AB-8 type macroporous adsorption resin column at 1.5-2.5 BV / h, eluting (removing strong polar impurities such as low eutectic solvents, sugars and proteins) with 3-5 BV of water (flow rate 2 BV / h), and then eluting with 4-6 BV of 68%-72% ethanol solution (flow rate 1-2 BV / h), collecting the eluent of the 68%-72% ethanol solution, and vacuum freeze-drying the eluent to a water content of 2wt%-5wt% to obtain the Jasminum mesnyi flower extract. The preparation method of the eutectic solvent aqueous solution preferably comprises: mixing choline chloride and glycerol at a molar ratio of 1:1.5-2.5, stirring at 200-400 rpm and 70-80 ℃ for 50-60 min to obtain a eutectic solvent, mixing the eutectic solvent and water at a weight ratio of (7-8):(2-3), and stirring at 200-400 rpm for 5-10 min to obtain the eutectic solvent aqueous solution.

[0028] Compared with the traditional method, the Jasminum mesnyi flower extract is prepared by using a eutectic solvent in the present application, and a more environmentally friendly, efficient and mild method is used to obtain a Jasminum mesnyi flower extract with higher purity and higher activity; the Jasminum mesnyi flower extract contains rich lignans (such as magnolin and magnolol), volatile oil and flavonoids, has the effects of scavenging free radicals, antioxidation, inhibiting matrix metalloproteinase, protecting collagen and delaying cell aging, and also has the effects of anti-inflammatory, soothing, promoting barrier repair and inhibiting elastase. Therefore, the Jasminum mesnyi flower extract can effectively prevent and fade wrinkles, improve skin roughness and dullness, enhance skin tolerance, realize efficient anti-aging and repair, and the like.

[0029] This invention utilizes extracts from the bark of *Bretschneidera sinensis*, the fruit of *Annona squamosa*, and the flower of *Magnolia denudata* as active ingredients in an anti-aging composition. The combination of these three ingredients comprehensively scavenges free radicals, reduces oxidative damage, and enhances antioxidant effects. *Bretschneidera sinensis* bark extract reduces collagen degradation by inhibiting collagenase activity; *Magnolia denudata* flower extract specifically inhibits matrix metalloproteinases and elastases, preventing abnormal degradation of collagen and elastin; and *Annona squamosa* fruit extract promotes collagen and elastin synthesis. *Bretschneidera sinensis* bark extract can reduce skin yellowing and stiffness caused by the accumulation of advanced glycation end products (AGEs); *Magnolia denudata* flower extract can prolong the lifespan of epidermal cells and dermal fibroblasts; and *Annona squamosa* fruit extract can improve cellular energy metabolism, thereby… Delaying skin aging; the bark extract of *Bonbour glauca* can soothe nerve endings and reduce sensitivity symptoms such as redness and burning; the fruit extract of *Annona squamosa* can regulate the skin flora balance, reduce inflammation, and relieve itching and erythema; the flower extract of *Magnolia denudata* can quickly soothe acute inflammatory reactions and enhance skin immune tolerance. The three exert anti-inflammatory effects through multiple mechanisms such as inhibiting inflammatory factors, regulating flora, and enhancing tolerance; the fruit extract of *Annona squamosa* promotes the proliferation and differentiation of keratinocytes and accelerates the reconstruction of the stratum corneum; the flower extract of *Magnolia denudata* can enhance the barrier's water-locking ability; the bark extract of *Bonbour glauca* can reduce transdermal water loss, improve the dermal-epidermal junction structure, and enhance skin resilience. The combination of the three can achieve full structural reconstruction from the stratum corneum to the deep cells. As can be seen from the above, the combination of these three ingredients can construct a multi-dimensional antioxidant network, promoting collagen synthesis and inhibiting degradation to protect the supporting structure, activating longevity proteins to combat glycation and DNA damage, and comprehensively delaying skin aging (wrinkles, sagging, dullness); through multi-target anti-inflammatory and soothing effects, it can rebuild the barrier from the stratum corneum to the deep cells, supporting wound healing and improving tolerance, and is suitable for sensitive skin, damaged skin, and post-medical aesthetic care.

[0030] The anti-aging composition of the present invention preferably includes fibroin and mannan.

[0031] The anti-aging composition of the present invention preferably includes a moisturizer, an emulsifier, and a preservative, all of which are selected from raw materials known in the cosmetics field.

[0032] This invention also provides a method for preparing the anti-aging composition described above, comprising: mixing recombinant human type III collagen, *Bretschneidera sinensis* bark extract, *Magnolia denudata* flower extract, *Annona squamosa* fruit extract, rhamnose, trimethylpentanediol / adipic acid / glycerol crosspolymer, and water, and homogenizing to obtain the anti-aging composition. The homogenization speed is preferably 3000-4000 rpm, more preferably 3500 rpm, and the homogenization time is preferably 10-15 min, more preferably 12 min.

[0033] The application also provides a use of the composition in the preparation of anti-aging and repair products.

[0034] The technical solutions in the present application will be clearly and completely described below in combination with the embodiments in the present application. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0035] In the following examples, all are conventional methods unless otherwise specified.

[0036] In the following examples, all the materials and reagents used are commercially available unless otherwise specified.

[0037] Some raw materials and sources are as follows: The bark extract of Malpighia emarginata DC. is Malpighia emarginata DC. bark extract 1 in CN119837808B; Lactobacillus plantarum WCFS1 is purchased from China Center for Type Culture Collection, with the preservation number of CCTCC NO:M2021228, and is disclosed in CN115305217A; Bifidobacterium animalis W3 is purchased from China General Microbiological Culture Collection Center, with the preservation number of CGMCC No.25262, and is disclosed in CN117224462A; Recombinant human type III collagen is purchased from Xi'an Baiwangda Pharmaceutical Technology Co., Ltd.; Rhamnose is L-rhamnose, purchased from Xi'an Nanpu Biological Technology Co., Ltd.; Trimethylpentanediol / adipic acid / glycerin crosspolymer is purchased from Shanghai Zibang Biological Medicine Co., Ltd.; Lactobacillus delbrueckii WHH3887 is purchased from China General Microbiological Culture Collection Center, with the preservation number of CGMCC No.20090, and is disclosed in CN112625971A; Bifidobacterium adolescentis ZJ2 is purchased from China General Microbiological Culture Collection Center, with the preservation number of CGMCC NO.18901, and is disclosed in CN112725219A; Hydroxyl radical scavenging capacity detection kit, ABTS radical scavenging capacity detection kit, and superoxide anion scavenging capacity detection kit are all purchased from Beijing Solabio Science and Technology Co., Ltd.; Mouse superoxide dismutase (SOD) ELISA detection kit, mouse glutathione peroxidase (GSH-Px) ELISA detection kit, mouse catalase (CAT) ELISA detection kit, mouse malondialdehyde (MDA) ELISA detection kit are purchased from Shanghai Kai Bo Biotechnology Co., Ltd.

[0038] Example 1 Preparation of anti-aging composition (1) Preparation of annona glabra fruit extract The fresh annona glabra fruit was subjected to intermittent crushing mode of a cell disrupter at 25000 rpm for 30 s, paused for 30 s, repeated for 4 times, to obtain a slurry, 15 times slurry weight of water was added, sterilized, inoculated with composite microorganisms, anaerobic fermentation at 37℃, pH 6.5 for 64 h, sterilized by ultraviolet lamp, 0.22 μm membrane filtration, vacuum freeze-drying of the filtrate to a water content of 3wt%, to obtain annona glabra fruit extract; Among them, the composite microorganisms are composed of lactobacillus plantarum WCFS1 and animal bifidobacterium W3, the number of lactobacillus plantarum WCFS1 in the composite microorganisms is 8×10 8 Individual / g, the number of animal bifidobacterium W3 is 5×10 8 Individual / g, and the inoculation amount of the composite microorganisms is 4% of the slurry weight.

[0039] (2) Preparation of jasminum mesnyi flower extract The dried jasminum mesnyi flower buds were crushed to pass through an 80 mesh sieve to obtain jasminum mesnyi flower powder, which was added into a eutectic solvent aqueous solution at a ratio of 1:18 g / mL, extracted at 65℃, 300 rpm for 75 min, cooled to 28℃, centrifuged at 9000 rpm for 12 min to obtain supernatant; the supernatant was adsorbed on an AB-8 type macroporous adsorption resin column at a flow rate of 2BV / h, then eluted with 4BV of water at a flow rate of 2BV / h, and then eluted with 5BV of 70% ethanol solution at a flow rate of 1.5BV / h, and the eluate was collected, and the eluate was vacuum freeze-dried to a water content of 3wt% to obtain jasminum mesnyi flower extract; Among them, the preparation of eutectic solvent aqueous solution: choline chloride and glycerol were mixed at a molar ratio of 1:2, stirred at 300 rpm, 75℃ for 55 min to obtain eutectic solvent, and then mixed with water at a weight ratio of 7.5:2.5, stirred at 300 rpm for 8 min to obtain eutectic solvent aqueous solution.

[0040] (3) Preparation of anti-aging composition Accurately weigh: recombinant human type III collagen 0.15 parts, Malabark tree bark extract 0.08 parts, annona glabra fruit extract 0.02 parts, jasminum mesnyi flower extract 0.05 parts, rhamnose 0.8 parts, trimethylpentanediol / adipic acid / glycerin crosspolymer 1.2 parts, glycerin 4 parts, squalane / caprylic capric triglyceride 8 parts, steareth-21 2 parts, steareth-2 0.8 parts, phenoxyethanol 0.5 parts, ethylhexylglycerin 0.25 parts, and deionized water in balance, based on 100 parts by weight; Glycerin, rhamnose, trimethylpentanediol / adipic acid / glycerin crosspolymer, and deionized water were stirred at 72°C and 250 rpm for 18 min to obtain an aqueous phase; squalane / caprylic capric triglyceride, steareth-21, and steareth-2 were dissolved at 78°C for 8 min to obtain an oil phase; the oil phase was mixed with the aqueous phase, homogenized at 3500 rpm for 12 min to obtain a primary emulsion, and then cooled to 40°C; Malabark tree bark extract solution, annona glabra fruit extract solution, jasminum mesnyi flower extract solution, recombinant human type III collagen, phenoxyethanol, and ethylhexylglycerin were added, and the pH value was adjusted to 6.2 to obtain an anti-aging composition.

[0041] Example 2 Preparation of the anti-aging composition (1) Preparation of annona glabra fruit extract Fresh annona glabra fruits were subjected to intermittent crushing mode of a homogenizer at 20000 rpm for 30 s, paused for 30 s, and repeated for 4 times to obtain a slurry. Water was added at 12 times the weight of the slurry, sterilized, inoculated with a composite microorganism, and subjected to anaerobic fermentation at 35°C and pH 6 for 72 h. The fermentation product was sterilized by ultraviolet light and filtered through a 0.22 μm membrane. The filtrate was vacuum freeze-dried to a water content of 2 wt% to obtain annona glabra fruit extract. The composite microorganism was composed of Lactobacillus plantarum WCFS1 and Bifidobacterium animalis W3. The number of Lactobacillus plantarum WCFS1 in the composite microorganism was 6×10 8 The number of Bifidobacterium animalis W3 was 3×10 8 The inoculation amount of the composite microorganism was 5% of the weight of the slurry.

[0042] (2) Preparation of jasminum mesnyi flower extract The dried Jasminum mesnyi flower buds were crushed through a 50-mesh screen to obtain Jasminum mesnyi powder, which was extracted with a eutectic solvent aqueous solution at 60°C and 200 rpm for 90 min, and then centrifuged at 8000 rpm for 15 min after cooling to 25°C to obtain a supernatant; the supernatant was adsorbed on an AB-8 type macroporous adsorption resin column at 1.5 BV / h, eluted with 3 BV of water (flow rate 2 BV / h), and then eluted with 4 BV of a 68% ethanol solution (flow rate 1 BV / h), and the eluate of the 68% ethanol solution was collected and vacuum freeze-dried to a water content of 2 wt% to obtain a Jasminum mesnyi flower extract; The eutectic solvent aqueous solution was prepared by mixing choline chloride and glycerol at a molar ratio of 1:1.5, stirring at 200 rpm and 70°C for 60 min to obtain a eutectic solvent, and then mixing the eutectic solvent with water at a weight ratio of 7:3 and stirring at 200 rpm for 10 min.

[0043] (3) Preparation of an anti-aging composition The following ingredients were accurately weighed in 100 parts by weight: recombinant human collagen type III 0.05 parts, extract of Tabernaemontana pandacaqui bark 0.05 parts, extract of Anonidium venceyense fruit 0.03 parts, extract of Jasminum mesnyi flower 0.03 parts, rhamnose 1 part, trimethylpentanediol / adipic acid / glycerin crosspolymer 1.5 parts, panthenol 1.5 parts, squalane 5 parts, PEG-100 stearate 2 parts, benzyl alcohol 0.8 parts, and deionized water in the balance. The panthenol, rhamnose, trimethylpentanediol / adipic acid / glycerin crosspolymer, and deionized water were stirred at 70°C and 200 rpm for 20 min to obtain an aqueous phase; the squalane and PEG-100 stearate were dissolved at 75°C for 10 min to obtain an oil phase; the oil phase and the aqueous phase were mixed and homogenized at 3000 rpm for 15 min to obtain a primary emulsion, and then the pH value was adjusted to 6 by adding the extract of Tabernaemontana pandacaqui bark solution, the extract of Anonidium venceyense fruit solution, the extract of Jasminum mesnyi flower solution, the recombinant human collagen type III, and the benzyl alcohol to obtain an anti-aging composition.

[0044] Example 3 Preparation of an anti-aging composition (1) Preparation of an extract of Anonidium venceyense fruit The fresh Anonidium venceyense fruit was subjected to intermittent crushing mode of a homogenizer at 30000 rpm for 30 s, paused for 30 s, and repeated for 3 times to obtain a slurry, 17 times the weight of water was added to the slurry, sterilized, inoculated with a composite microorganism, and subjected to anaerobic fermentation at 40°C and pH 7 for 56 h, sterilized by ultraviolet light irradiation, and then filtered through a 0.22 μm membrane to obtain a filtrate, which was vacuum freeze-dried to a water content of 4 wt% to obtain an extract of Anonidium venceyense fruit. The complex microorganism is composed of Lactobacillus plantarum WCFS1 and Bifidobacterium animalis W3, the number of Lactobacillus plantarum WCFS1 in the complex microorganism is 1×10 9 The number of Bifidobacterium animalis W3 is 7×10 8 The inoculation amount of the complex microorganism is 3% of the weight of the serum.

[0045] (2) Preparation of Jasminum mesnyi flower extract The dried Jasminum mesnyi flower buds are crushed through a 100-mesh screen to obtain Jasminum mesnyi powder, which is added into a eutectic solvent aqueous solution at a ratio of 1:20 g / mL, extracted at 70℃ for 60 min under 400 rpm, cooled to 30℃, and centrifuged at 10,000 rpm for 10 min to obtain a supernatant; the supernatant is adsorbed by an AB-8 type macroporous adsorption resin column at a flow rate of 2.5 BV / h, eluted with 5 BV of water (flow rate 2 BV / h), and then eluted with 6 BV of 72% ethanol solution (flow rate 2 BV / h), and the eluate of the 72% ethanol solution is collected and vacuum freeze-dried to a water content of 3 wt% to obtain the Jasminum mesnyi flower extract. The eutectic solvent aqueous solution is prepared by mixing choline chloride and glycerol at a molar ratio of 1:2.5, stirring at 400 rpm and 80℃ for 50 min to obtain a eutectic solvent, and then mixing the eutectic solvent with water at a weight ratio of 8:2 and stirring at 400 rpm for 5 min.

[0046] (3) Preparation of anti-aging composition Accurately weigh 0.18 parts of recombinant human collagen type III, 0.1 parts of Tabernaemontana pandacaqui bark extract, 0.01 parts of Mimusops elengi fruit extract, 0.08 parts of Jasminum mesnyi flower extract, 0.5 parts of rhamnose, 1 part of trimethylpentanediol / adipic acid / glycerin crosspolymer, 3 parts of glycerin, 2 parts of vaseline, 1.5 parts of lanolin, 4 parts of caprylic / capric triglyceride, 1.2 parts of cetostearyl alcohol polyether-20, 1 part of cetostearyl alcohol, 0.5 parts of phenoxyethanol, 0.3 parts of ethylhexylglycerin, and the balance of deionized water, based on 100 parts by weight. Stir the glycerin, rhamnose, trimethylpentanediol / adipic acid / glycerin crosspolymer, and deionized water at 75℃ and 300 rpm for 15 min to obtain an aqueous phase; dissolve the vaseline, lanolin, caprylic / capric triglyceride, cetostearyl alcohol polyether-20, and cetostearyl alcohol in the oil phase at 78℃ for 8 min; mix the oil phase and the aqueous phase, homogenize at 4000 rpm for 10 min to obtain a preliminary emulsion, and then cool to 40℃, add the Tabernaemontana pandacaqui bark extract solution, Mimusops elengi fruit extract solution, Jasminum mesnyi flower extract solution, recombinant human collagen type III, phenoxyethanol, and ethylhexylglycerin, and adjust the pH value to 6.5 to obtain the anti-aging composition.

[0047] Comparative Example 1 Different from Example 1, the recombinant human type III collagen was abandoned, the weight fraction of rhamnose was adjusted to 0.86 parts, and the weight fraction of trimethylpentanediol / adipic acid / glycerin crosslinking polymer was adjusted to 1.29 parts.

[0048] Comparative Example 2 Different from Example 1, the rhamnose and trimethylpentanediol / adipic acid / glycerin crosslinking polymer were abandoned, and the weight fraction of recombinant human type III collagen was adjusted to 2.15 parts.

[0049] Comparative Example 3 Different from Example 1, the recombinant human type III collagen was replaced by laurocapram.

[0050] Comparative Example 4 Different from Example 1, the complex microorganism in step (1) was composed of Lactobacillus delbrueckii WHH3887 and Bifidobacterium adolescentis ZJ2, the number of Lactobacillus delbrueckii WHH3887 in the complex microorganism was 8×10 8 cells / g, the number of Bifidobacterium adolescentis ZJ2 was 5×10 8 cells / g, and the inoculation amount of the complex microorganism was 4% of the weight of the serum, and the other conditions were unchanged.

[0051] Comparative Example 5 Different from Example 1, the aqueous eutectic solvent solution in step (2) was replaced by an ethanol solution with a volume fraction of 70%.

[0052] Test Example 1 In vitro antioxidant assay The anti-aging compositions of Examples 1-3 and Comparative Examples 1-5 were mixed with deionized water at a volume ratio of 1:1 to obtain the test samples.

[0053] The hydroxyl radical scavenging rate, ABTS + radical scavenging rate, and superoxide radical scavenging rate of the test samples of Examples 1-3 and Comparative Examples 1-5 were determined, respectively.

[0054] The hydroxyl radical scavenging rate, ABTS + radical scavenging rate, and superoxide radical scavenging rate were detected by spectrophotometry according to the kit instructions.

[0055] The hydroxyl radical scavenging rate, ABTS + radical scavenging rate, and superoxide radical scavenging rate of the test samples of the anti-aging compositions of Examples 1-3 and Comparative Examples 1-5 are shown in Table 1.

[0056] Table 1 Antioxidant capacity of anti-aging compositions of each group Grouping Hydroxyl radical scavenging rate (%) ABTS+ free radical scavenging rate (%) Superoxide radical scavenging rate (%) Example 1 92.52 94.27 88.33 Example 2 90.17 92.53 86.71 Example 3 91.31 93.16 87.58 Comparative Example 1 69.80 71.51 67.90 Comparative Example 2 82.65 86.82 81.86 Comparative Example 3 75.98 77.68 73.54 Comparative Example 4 80.24 82.35 78.12 Comparative Example 5 83.56 85.14 81.45 The results of Table 1 show that the antioxidant capacity of Examples 1-3 is the strongest. It can be seen from the comparison of Example 1 and Comparative Examples 1-3 that the antioxidant performance of the discarded or replaced recombinant human type III collagen is reduced, indicating that the recombinant human type III collagen has certain antioxidant performance. It can be seen from the comparison of Example 1 and Comparative Examples 4-5 that the specific complex microorganism has an effect on the activity of the fermentation extract, and the deep eutectic solvent extraction is more efficient and can improve the antioxidant performance of the extract.

[0057] Test Example 2 Natural aging animal experiment SPF level 8-week-old 18-22g Kunming mice were selected, and after adaptive feeding for 1 week, the hair on the nape of the neck of the mice was shaved off with an area of about 2cm x 2cm. After 72h of shaving, the mice were evenly divided into 11 groups, 10 in each group, half male and half female, and there was no significant difference in the body weight, health status, etc. of the mice in each group.

[0058] The grouping situation is as follows: The blank control group: 200mg / kg of physiological saline was subcutaneously injected on the nape of the neck every day, and 0.1mL of distilled water was applied to the shaved area every day; The model group: 200mg / kg of D-galactose was injected on the nape of the neck every day, and 0.1mL of distilled water was applied to the shaved area every day; The positive control group: 200mg / kg of D-galactose was injected on the nape of the neck every day, and 0.1mL of 5% vitamin E cream was applied to the shaved area every day; The Example 1-3 group: 200mg / kg of D-galactose was injected on the nape of the neck every day, and 0.1mL of the corresponding anti-aging composition was applied to the shaved area every day.

[0059] The Comparative Example 1-5 group: 200mg / kg of D-galactose was injected on the nape of the neck every day, and 0.1mL of the corresponding anti-aging composition was applied to the shaved area every day.

[0060] After 8 weeks of continuous injection and application, the mice were sacrificed after the last application, and the blood was taken from the eyeball into a centrifuge tube, and after standing, it was centrifuged (3000rpm, 10min) to obtain serum for determination of malondialdehyde (MDA).

[0061] Subsequently, the mice were sacrificed, and the skin tissue in the shaved area was quickly cut off, washed with pre-cooled physiological saline to remove bloodstains and attachments, and dried with filter paper. 0.1g of skin tissue was mixed with 0.9mL of 4°C PBS solution (pH 7.4), homogenized at 3000rpm for 15s under ice bath conditions, and repeated 4 times to obtain skin tissue homogenate.

[0062] The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in the skin tissue homogenate liquid were detected according to the kit instructions, and the content of malondialdehyde (MDA) in the serum was detected.

[0063] The results of SOD activity, GSH-Px activity, CAT activity in the skin tissue and the content of MDA in the serum are shown in Table 2.

[0064] Table 2 SOD activity, GSH-Px activity, CAT activity and MDA content of mice in each group Group SOD (U / mL) GSH-Px (U / mL) CAT (U / mL) MDA (nmol / mL) Blank control group 98.27 903.23 11.32 5.37 Model group 55.18 437.81 5.02 13.80 Positive control group 90.61 836.12 10.20 5.66 Example 1 Group 93.45 842.67 10.57 5.55 Example 2 group 92.80 840.51 10.39 5.79 Example 3 Group 91.07 838.05 10.28 5.71 Comparative Example 1 72.24 694.98 7.36 9.24 Comparative Example 2 74.36 705.70 7.53 9.03 Comparative Example 3 77.92 721.34 7.95 8.89 Comparative Example 4 83.59 757.06 8.41 8.48 Comparative Example 5 86.73 769.49 8.74 7.91 The data in Table 2 show that the SOD activity, GSH-Px activity and CAT activity in the skin tissue of the model group are significantly reduced compared with the blank control group, indicating that the mouse natural aging model is successfully constructed. Examples 1-3 are close to or better than the positive control group, indicating that the composition of the application has good anti-aging ability. The comparison of Example 1 and Comparative Examples 1-2 shows that there is a synergistic effect between the recombinant human type III collagen and the film-forming agent. The two are compounded to promote the penetration of the effective ingredient, while controlling the release efficiency, and efficiently penetrating the effective ingredient into the skin. The comparison of Example 1 and Comparative Example 3 shows that a suitable penetration enhancer can improve the anti-aging effect of the body. The comparison of Example 1 and Comparative Examples 4-5 shows that the extraction of raw materials using specific complex microorganisms and solvents can affect the anti-aging effect.

[0065] Test Example 3 Photoaging animal experiment SPF level 8-week-old Kunming mice weighing 18-22 g were selected, and after adaptive feeding for 1 week, the hair on the neck and back of the mice was shaved off with an area of about 2 cm x 2 cm. After 72 h of shaving, the mice were evenly divided into 11 groups, 10 mice in each group, half male and half female, and there was no significant difference in body weight, health status, etc. between the mice in each group.

[0066] The grouping situation is as follows: Blank control group: 1 h after ultraviolet irradiation every day, 0.1 mL of distilled water was applied to the shaved part; Model group: 1 h after ultraviolet irradiation every day, 0.1 mL of distilled water was applied to the shaved part; Positive control group: 1 h after ultraviolet irradiation every day, 0.1 mL of 5% vitamin E cream was applied to the shaved part; Example 1-3 group: 1 h after ultraviolet irradiation every day, 0.1 mL of the corresponding anti-aging composition was applied to the shaved part.

[0067] Comparative Examples 1-5 group: 1 h after ultraviolet irradiation every day, 0.1 mL of the corresponding anti-aging composition was applied to the shaved part.

[0068] The ultraviolet irradiation method is: UVA and UVB combined ultraviolet irradiation, the irradiation dose ratio of UVA and UVB is 9:1, irradiation once a day, the dose of combined ultraviolet irradiation is 100 mJ / cm 2 in the first week, the dose is 200 mJ / cm 2 in the second week, the dose is 300 mJ / cm 2 in the third week, and the dose is 400 mJ / cm 2 .

[0069] (1) Macroscopic score After 24 hours of applying the corresponding composition at the eighth week, at least five researchers who do not know the grouping situation score the irradiated area of the depilated back of the mice under standard lighting conditions in a blind manner, the scoring criteria are shown in Table 3, and the scoring results are shown in Table 4.

[0070] Table 3 Macroscopic scoring criteria wrinkle erythema Scales / Desquamation score Skin is smooth and firm, without any visible wrinkles. The skin color was normal, with no difference from the un-irradiated areas, and no signs of redness. The skin surface is free of any scales or dry flakes. 0 The skin is slightly rough, with extremely fine and sparse superficial lines. The skin is light pink with a slight redness. Slightly dry skin with fine powdery flakes 1 Clear but shallow wrinkles are visible; the lines are independent of each other and do not form a network. The skin appears distinctly pink or has localized mild redness. Dry skin with visible fine white scales 2 Wrinkles have deepened significantly, some forming incomplete mesh-like patterns. Skin is sagging. The skin is dark red or the red area is expanding. Extensive skin desquamation, with large, white or grayish-white scales visible on the skin. 3 Deep, coarse wrinkles cover the surface, forming a dense, rough, net-like or grooved pattern, giving it a leather-like appearance. The skin is deep red to purplish-red, with a rich color, and may be accompanied by slight swelling. —— 4 Table 4 Scoring of mice in each group Group Wrinkles (divided) Erythema (divided) Scales / Desquamation (classified) Total score (points) Blank control group 0 0 0 0 Model group 4.5 4.4 3.1 12.0 Positive control group 1.6 1.5 1.4 4.5 Example 1 Group 1.0 1.1 1.2 3.3 Example 2 group 1.1 1.4 1.3 3.8 Example 3 Group 1.3 1.2 1.3 3.8 Comparative Example 1 2.1 2.2 2.0 6.3 Comparative Example 2 2.3 2.0 1.8 6.1 Comparative Example 3 1.8 3.7 1.7 7.2 Comparative Example 4 1.9 1.8 1.5 5.2 Comparative Example 5 1.9 1.6 1.6 5.1 The results in Table 4 show that the model group has a significantly higher score in wrinkles, erythema and scales / desquamation than the blank control group, indicating that the photoaging mouse model is successfully constructed. The effects of Examples 1-3 are higher than those of the positive control group, indicating that the composition of the application has a significant effect on anti-photoaging. As can be seen from the comparison between Example 1 and Comparative Examples 1-2, the recombinant human type III collagen and the film-forming agent have a synergistic effect, which can relieve the symptoms of skin wrinkles, erythema and scales / desquamation caused by ultraviolet light. As can be seen from the comparison between Example 1 and Comparative Example 3, the erythema score is the highest, indicating that the recombinant human type III collagen has lower skin irritation than laurocapram. As can be seen from the comparison between Example 1 and Comparative Examples 4-5, different complex microorganisms and solvents can affect the anti-photoaging effect of raw material extraction.

[0071] (2) Skin index After the mice are scored, the eyeballs are enucleated to collect blood in centrifuge tubes, which are centrifuged (3000 rpm, 10 min) after standing, and serum is separated for determination of malondialdehyde (MDA).

[0072] Subsequently, the mice are sacrificed, the skin tissue of the shaved area is quickly cut off, washed with pre-cooled normal saline to remove bloodstains and attachments, and dried with filter paper. 0.1 g of skin tissue is mixed with 0.9 mL of 4°C PBS solution (pH 7.4), homogenized at 3000 rpm under ice bath conditions for 15 s, and repeated 4 times to obtain skin homogenate.

[0073] The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in the homogenate of skin tissue were detected, and the content of malondialdehyde (MDA) in serum was detected.

[0074] The activity of SOD was detected by hydroxylamine method, the activity of GSH-Px was detected by colorimetry, the activity of CAT was detected by ammonium molybdate method, and the content of MDA was detected by thiobarbituric acid method.

[0075] The results of the activities of SOD, GSH-Px and CAT in skin tissue and the content of MDA in serum of the mice in each group are shown in Table 5.

[0076] Table 5: SOD activity, GSH-Px activity, CAT activity and MDA content of mice in each group Group SOD (U / mL) GSH-Px (U / mL) CAT (U / mL) MDA (nmol / mL) Blank control group 96.23 896.63 11.53 5.15 Model group 52.65 428.27 4.81 14.23 Positive control group 88.91 821.82 10.38 5.81 Example 1 Group 86.08 830.58 10.17 5.69 Example 2 group 88.54 824.06 10.22 5.73 Example 3 Group 88.34 835.91 10.49 5.57 Comparative Example 1 70.39 683.78 7.15 9.30 Comparative Example 2 72.82 692.80 7.30 9.24 Comparative Example 3 68.16 677.14 6.92 9.72 Comparative Example 4 80.70 759.49 8.24 8.08 Comparative Example 5 82.47 765.35 8.56 7.86 The results in Table 5 show that the activities of SOD, GSH-Px and CAT in skin tissue of the model group are significantly lower than those of the blank control group, indicating that the mouse photoaging model is successfully constructed. The compositions in Examples 1-3 are better than the positive control group, indicating that the compositions have good anti-photoaging effect. The comparison between Example 1 and Comparative Examples 1-2 shows that the recombinant human type III collagen and the film-forming agent have a synergistic effect. The two are compounded to promote the penetration of the effective components, control the release efficiency, efficiently penetrate the effective components into the skin, and improve the anti-photoaging effect. The comparison between Example 1 and Comparative Example 3 shows that a suitable penetration enhancer can improve the anti-photoaging effect of the body. The comparison between Example 1 and Comparative Examples 4-5 shows that the extraction of raw materials by specific complex microorganisms and solvents can affect the anti-photoaging effect.

[0077] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. An anti-aging composition for improving the permeation effect of an extract from the bark of a tree of the genus Eupatorium, characterized in that, The composition comprises the following raw materials by weight: 0.05-0.2 parts of recombinant human type III collagen, 0.05-0.1 parts of extract of Symplocos paniculata tree bark, 0.01-0.03 parts of extract of Annona glabra fruit, 0.03-0.08 parts of extract of Jasminum mesnyi flower, and 1.5-2.5 parts of a film-forming agent. The film-forming agent is composed of rhamnose and trimethylpentanediol / adipic acid / glycerol cross-linked polymer.

2. The anti-aging composition according to claim 1, characterized by, The weight ratio of the rhamnose and trimethylpentanediol / adipic acid / glycerol cross-linked polymer is (0.5-1):(1-1.5).

3. The anti-aging composition according to claim 1, wherein The extract of Annona glabra fruit is obtained by anaerobic fermentation of Annona glabra fruit with complex microorganisms.

4. The anti-aging composition according to claim 3, characterized by, The complex microorganisms are composed of Lactobacillus plantarum WCFS1 and Bifidobacterium animalis W3.

5. The anti-aging composition according to claim 1, wherein The extract of Jasminum mesnyi flower is obtained by extraction of Jasminum mesnyi flower buds with a deep eutectic solvent and purification with a macroporous adsorption resin.

6. The anti-aging composition according to claim 5, wherein The deep eutectic solvent is composed of choline chloride and glycerol.

7. A method of preparing the anti-aging composition according to any one of claims 1 to 6, characterized in that, The composition comprises: The recombinant human type III collagen, the extract of Symplocos paniculata tree bark, the extract of Jasminum mesnyi flower, the extract of Annona glabra fruit, rhamnose, trimethylpentanediol / adipic acid / glycerol cross-linked polymer, and water are mixed and homogenized to obtain an anti-aging composition.

8. The preparation method according to claim 7, characterized in that, The rotation speed of the homogenization is 3000-4000 rpm, and the time is 10-15 min.

9. Use of the composition according to any one of claims 1-6 in the preparation of an anti-aging and repair product.

10. Use according to claim 9, characterized in that, The aging is photoaging and natural aging.