Preparation method of biological bacterial fertilizer

Abstract

The invention relates to the technical field of biological bacterial fertilizers, and particularly discloses a preparation method of a biological bacterial fertilizer. The preparation method of the biological bacterial fertilizer comprises the following steps: (1) mixing agricultural and forestry wastes and livestock and poultry manure according to a mass ratio of (3-5): (1-1.2), performing primary screening, and adjusting the moisture content to 50-60%; (2) adding a compound microbial agent, and uniformly mixing to obtain a mixture; (3) performing enzymolysis treatment and dynamic fermentation on the mixture to prepare coarse fertilizer grains; (4) crushing and granulating the fertilizer coarse grains, and screening to obtain the biological bacterial fertilizer; in addition, the preparation method disclosed by the invention has the advantages of improving the organic matter degradation efficiency and the fertilizer efficiency.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of biological bacterial fertilizer, and more particularly to a preparation method of biological bacterial fertilizer. BACKGROUND

[0002] With the continuous development of agriculture and environmental protection awareness, the application of biological bacterial fertilizer in agricultural production is increasingly valued. Biological bacterial fertilizer not only can improve soil structure and increase soil fertility, but also can reduce the use of chemical fertilizers and reduce environmental pollution, which is of great significance to the sustainable development of agriculture. It can promote the growth of crops, improve the yield and quality of crops, and play a key role in ensuring food security and agricultural product quality. Moreover, rational use of biological bacterial fertilizer helps to promote the construction of ecological agriculture and achieve resource recycling and ecological environment protection.

[0003] In the field of biological bacterial fertilizer preparation, in order to solve the problem of biological bacterial fertilizer preparation, various means are commonly used. Commonly used is to directly use single organic material, such as simply using agricultural and forestry waste or livestock and poultry manure, and simply stacking and fermenting. This method is relatively simple to operate and has low cost, but the nutrient composition is not rich enough due to the single material. Another method is to randomly mix different organic materials without considering their proportion, and then naturally ferment. In addition, some traditional methods lack effective use of microbial inoculants during fermentation, and only rely on naturally occurring microorganisms for fermentation, which is slow and unstable in fermentation effect. In addition, the moisture content, temperature and other conditions are not precisely controlled during the fermentation process, resulting in uneven quality of the final product.

[0004] However, the existing biological bacterial fertilizer preparation method has obvious defects. The traditional method does not have a scientific quality ratio when mixing materials, resulting in unbalanced nutrient composition and failing to meet the diversified needs of crop growth. At the same time, the lack of reasonable addition of compound microbial inoculants and precise control of fermentation parameters makes the fermentation process difficult to proceed fully, and the biological bacterial fertilizer has low fertilizer efficiency, which cannot achieve the ideal effect of increasing yield and improving soil. SUMMARY

[0005] In order to improve the degradation efficiency of organic matter and the fertilizer efficiency, the application provides a preparation method of biological bacterial fertilizer.

[0006] In a first aspect, the application provides a preparation method of biological bacterial fertilizer, which adopts the following technical scheme: A preparation method of biological bacterial fertilizer, comprising the following steps: (1) mixing agricultural and forestry waste and livestock and poultry manure according to a mass ratio of (3-5):(1-1.2), adjusting the moisture content to 50-60% after preliminary screening; (2) adding a compound microbial agent, and obtaining a mixture after mixing uniformly; (3) performing enzymatic hydrolysis treatment and dynamic fermentation on the mixture to obtain a coarse fertilizer granule; (4) performing crushing and granulation on the coarse fertilizer granule, and performing screening to obtain a biological bacterial fertilizer.

[0007] By adopting the above technical solution, the agricultural and forestry wastes and the wastes of the agricultural and forestry and animal husbandry are reused, the cellulose, lignin and other difficult-to-ferment-degrade substances in the raw materials are decomposed through enzymatic hydrolysis treatment, the degradation efficiency of the organic matter and the product fertilizer efficiency are improved, the enzymatic hydrolysis products are fully fermented through the compound microbial agent, and the activity of the fermented products is high.

[0008] Preferably, the compound microbial agent comprises the microbial agent and bran at a mass ratio of 1:(9.5-10.5), the microbial agent comprises Bacillus subtilis, actinomycete, Trichoderma and photosynthetic bacteria at a mass ratio of (3-4):(2-3):(1.5-2.5):(1-1.5), and the addition amount of the compound microbial agent is 0.5-1wt% of the mixture.

[0009] By adopting the above technical solution, the Bacillus subtilis, actinomycete, Trichoderma and photosynthetic bacteria are compounded to synthesize the synergistic compound microbial agent, the fermentation efficiency of the raw materials and the activity of the fermentation products are effectively improved, and the fermentation period is shortened, and the growth of pathogenic bacteria can be inhibited.

[0010] Preferably, the enzymatic hydrolysis treatment comprises the following steps: adding a mixed enzyme preparation at 45-55℃, and performing constant-temperature enzymatic hydrolysis for 24-48h at pH 6-7.5, and the addition amount of the mixed enzyme preparation is 0.3-0.8wt% of the mixture.

[0011] By adopting the above technical solution, the temperature, pH and addition amount of the mixed enzyme of the enzymatic hydrolysis are controlled, so that the cellulose, lignin and other substances in the raw materials can be fully degraded, and the proteins are preliminarily degraded, the fermentation period is effectively shortened, and the fermentation is facilitated and the effect is improved.

[0012] Preferably, the mixed enzyme preparation comprises cellulase and protease at a mass ratio of (2-5):(1-3).

[0013] By adopting the above technical solution, the mass ratio of the cellulase and the protease is controlled, so that the mixed enzyme preparation can be fully enzymatically hydrolyzed to the cellulose, lignin and proteins in the raw materials, the protease can decompose the proteins in the plants to expose the cellulose and lignin, which is beneficial to the enzymatic hydrolysis, and the small-molecule nutrient substances generated by the enzymatic hydrolysis are beneficial to the reproduction of the bacterial flora, and a good foundation is provided for the enzymatic hydrolysis treatment.

[0014] Preferably, the dynamic fermentation comprises the following steps: turning over every 8 hours, maintaining the temperature at 30-40 DEG C, and the duration is 7-10 days until the composting.

[0015] By adopting the technical scheme, sufficient oxygen supply is ensured by turning over, the temperature is controlled, the activity of the bacteria is promoted, the damage of high temperature to the bacteria is reduced, the fermentation time is short, C / N≤20, and the germination index is ≥90%, so that sufficient nitrogen elements are provided for crops, and the growth of crops is promoted.

[0016] Preferably, the bacterial agent is further treated as follows: the bacterial agent is added into water and uniformly dispersed to obtain a bacterial liquid for standby, sodium carboxymethyl starch and sodium caseinate are dissolved in water, and after being uniformly mixed, the homogeneous solution is obtained by standing at 4 DEG C for 24 hours, the bacterial liquid is added drop by drop while stirring, and the bacterial agent microcapsules are obtained by freeze-drying after being uniformly mixed.

[0017] By adopting the technical scheme, the bacterial agent is coated and the bacterial agent microcapsules are formed by using sodium carboxymethyl starch and sodium caseinate as the capsule wall, so that the bacterial agent is fully protected, the bacterial agent is not easily affected in the enzymatic hydrolysis process, and the activity of the bacterial agent in the fermentation process is maintained.

[0018] Preferably, the mass ratio of the sodium carboxymethyl starch and the sodium caseinate is (0.9-1.1):(5.6-5.9), and the concentration of the homogeneous solution is 1.5-2 g / mL.

[0019] By adopting the technical scheme, the mass ratio of the sodium carboxymethyl starch and the sodium caseinate is controlled, so that the embedding rate and the forming effect of the bacterial agent microcapsules are effectively controlled, the bacterial agent microcapsules are treated by the mixed enzyme preparation in the enzymatic hydrolysis process, the capsule wall is further destroyed, the bacterial agent as the core of the microcapsules is released at the end of the enzymatic hydrolysis process, and the next stage of fermentation is prepared.

[0020] Preferably, the biological bacterial fertilizer is further post-treated as follows: polyglutamic acid and chitosan are mixed and dissolved in an acetic acid solution, mixed at 70 DEG C for 2 hours to form a coating solution, and the biological bacterial fertilizer is subjected to fluidized bed coating treatment, and the coating thickness is 10-15 microns.

[0021] By adopting the technical scheme, the biological bacterial fertilizer after coating treatment has good slow-release performance, and the storage stability and application effect of the fertilizer are improved, the coating formed by polyglutamic acid and chitosan has good antibacterial performance, the activity of the biological bacterial fertilizer is maintained, and pollution is reduced.

[0022] In summary, the present application has the following beneficial effects: 1、Due to the reutilization of agricultural and forestry wastes and livestock manure and other wastes in the agricultural and forestry and animal husbandry industries in the present application, the difficult-to-ferment-degrade substances such as cellulose and lignin in the raw materials are decomposed through enzymatic hydrolysis treatment, the degradation efficiency of organic matter and the product fertilizer efficiency are improved, through the complex microbial agent, multiple strains synergistically act on each other, and the enzymatic hydrolysis products are fully fermented, and the activity of the fermented products is high.

[0023] 2、The Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria are compounded in the present application, and a synergistic complex microbial agent is composed, which can effectively improve the fermentation efficiency of the raw materials and the activity of the fermentation products, shorten the fermentation period, and inhibit the growth of pathogenic bacteria.

[0024] 3、The mass ratio of sodium carboxymethyl starch and sodium caseinate is controlled in the present application, which can effectively control the embedding rate and forming effect of the microbial agent microcapsules, the microbial agent microcapsules are treated by the mixed enzyme preparation during the enzymatic hydrolysis process, which can further destroy the capsule wall, so that the microbial agent as the core of the capsule can be released at the end of the enzymatic hydrolysis process, and prepare for the next stage of fermentation. DETAILED DESCRIPTION

[0025] The present application will be further described in detail below in combination with examples. Preparation examples 1-10 of the complex microbial agent

[0026] Preparation example 1 The complex microbial agent includes a microbial agent and bran in a mass ratio of 1:9.5, and the microbial agent includes Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria in a mass ratio of 3:2:1.5:1. The preparation method of the above complex microbial agent comprises the following steps: the microbial agent and the bran are fully mixed to obtain the complex microbial agent.

[0027] Preparation example 2 The complex microbial agent includes a microbial agent and bran in a mass ratio of 1:10.5, and the microbial agent includes Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria in a mass ratio of 4:3:2.5:1.5. The preparation method of the above complex microbial agent comprises the following steps: the microbial agent and the bran are fully mixed to obtain the complex microbial agent.

[0028] Preparation example 3 The difference between preparation example 3 and preparation example 1 is that in preparation example 3, the microbial agent includes Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria in a mass ratio of 3:1:3:0.5.

[0029] Preparation example 4 Preparation Example 4 is different from Preparation Example 1 in that the microbial agent in Preparation Example 4 comprises Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria in a mass ratio of 3:4:1:2.5.

[0030] Preparation Example 5 Preparation Example 5 is different from Preparation Example 1 in that the microbial agent in Preparation Example 5 is further treated by adding the microbial agent into water and uniformly dispersing to prepare 12 mL of microbial solution with a concentration of 1.21 x 10 5 CFU / mL for standby, dissolving carboxymethyl sodium starch and sodium caseinate in water, the mass ratio of carboxymethyl sodium starch and sodium caseinate being 0.9:5.6, uniformly mixing, and then obtaining 25 mL of homogeneous solution after standing at 4°C for 24 h, the concentration of the homogeneous solution being 1.5 g / mL, dropwise adding the microbial solution into the homogeneous solution while stirring, uniformly mixing, and then freeze-drying to obtain the microbial agent microcapsules.

[0031] Preparation Example 6 Preparation Example 6 is different from Preparation Example 1 in that the microbial agent in Preparation Example 6 is further treated by adding the microbial agent into water and uniformly dispersing to prepare 12 mL of microbial solution with a concentration of 1.52 x 10 5 CFU / mL for standby, dissolving carboxymethyl sodium starch and sodium caseinate in water, the mass ratio of carboxymethyl sodium starch and sodium caseinate being 1.1:5.9, uniformly mixing, and then obtaining 25 mL of homogeneous solution after standing at 4°C for 24 h, the concentration of the homogeneous solution being 2 g / mL, dropwise adding the microbial solution into the homogeneous solution while stirring, uniformly mixing, and then freeze-drying to obtain the microbial agent microcapsules.

[0032] Preparation Example 7 Preparation Example 7 is different from Preparation Example 5 in that the mass ratio of carboxymethyl sodium starch and sodium caseinate in Preparation Example 7 is 0.9:4.5.

[0033] Preparation Example 8 Preparation Example 8 is different from Preparation Example 5 in that the mass ratio of carboxymethyl sodium starch and sodium caseinate in Preparation Example 8 is 1.1:6.2.

[0034] Preparation Example 9 Preparation Example 9 is different from Preparation Example 5 in that the concentration of the homogeneous solution in Preparation Example 9 is 1 g / mL.

[0035] Preparation Example 10 Preparation Example 10 is different from Preparation Example 5 in that the concentration of the homogeneous solution in Preparation Example 10 is 2.5 g / mL. Embodiment

[0036] Embodiment 1 A preparation method of a biological microbial fertilizer, characterized in that the method comprises the following steps: (1) mixing the agricultural and forestry waste and livestock manure according to the mass ratio of 3:1, adjusting the moisture content to 60% after primary screening; (2) adding the compound microbial agent, obtaining a mixture after uniform mixing, the addition amount of the compound microbial agent is 0.5wt% of the mixture, and the compound microbial agent is the compound microbial agent prepared in Preparation Example 1; (3) performing enzymatic treatment and dynamic fermentation on the mixture to prepare the coarse granular fertilizer, the enzymatic treatment includes the following steps: adding a mixed enzyme preparation at 45℃, the mixed enzyme preparation includes cellulase and protease at a mass ratio of 2:1, constant temperature enzymolysis for 48h, pH is 7.5, and the addition amount of the mixed enzyme preparation is 0.3wt% of the mixture, and the dynamic fermentation includes the following steps: turning over once every 8 hours, maintaining the temperature at 30℃, and continuing for 10 days until rotting; (4) performing crushing and granulation on the coarse granular fertilizer, and screening to obtain the bio-fertilizer with a particle size of 5mm.

[0037] Example 2

[0038] A preparation method of a bio-fertilizer, characterized in that the method comprises the following steps: (1) mixing the agricultural and forestry waste and livestock manure according to the mass ratio of 5:1.2, adjusting the moisture content to 50% after primary screening; (2) adding the compound microbial agent, obtaining a mixture after uniform mixing, the addition amount of the compound microbial agent is 1wt% of the mixture, and the compound microbial agent is the compound microbial agent prepared in Preparation Example 2; (3) performing enzymatic treatment and dynamic fermentation on the mixture to prepare the coarse granular fertilizer, the enzymatic treatment includes the following steps: adding a mixed enzyme preparation at 55℃, the mixed enzyme preparation includes cellulase and protease at a mass ratio of 5:3, constant temperature enzymolysis for 48h, pH is 7.5, and the addition amount of the mixed enzyme preparation is 0.8wt% of the mixture, and the dynamic fermentation includes the following steps: turning over once every 8 hours, maintaining the temperature at 40℃, and continuing for 7 days until rotting; (4) performing crushing and granulation on the coarse granular fertilizer, and screening to obtain the bio-fertilizer with a particle size of 5mm.

[0039] Example 3

[0040] The difference between Example 3 and Example 1 is that, in Example 3, the addition amount of the compound microbial agent is 0.1wt% of the mixture.

[0041] Example 4

[0042] The difference between Example 4 and Example 1 is that, in Example 4, the addition amount of the compound microbial agent is 1.5wt% of the mixture.

[0043] Example 5

[0044] Example 5 differs from Example 1 in that, in Example 5, the complex microbial agent is the complex microbial agent prepared in Preparation Example 3.

[0045] Example 6

[0046] Example 6 differs from Example 1 in that, in Example 6, the complex microbial agent is the complex microbial agent prepared in Preparation Example 4.

[0047] Example 7

[0048] Example 7 differs from Example 1 in that, in Example 7, the complex microbial agent is the complex microbial agent prepared in Preparation Example 5.

[0049] Example 8

[0050] Example 8 differs from Example 1 in that, in Example 8, the complex microbial agent is the complex microbial agent prepared in Preparation Example 6.

[0051] Example 9

[0052] Example 9 differs from Example 1 in that, in Example 9, the complex microbial agent is the complex microbial agent prepared in Preparation Example 7.

[0053] Example 10

[0054] Example 10 differs from Example 1 in that, in Example 10, the complex microbial agent is the complex microbial agent prepared in Preparation Example 8.

[0055] Example 11

[0056] Example 11 differs from Example 1 in that, in Example 11, the complex microbial agent is the complex microbial agent prepared in Preparation Example 9.

[0057] Example 12

[0058] Example 12 differs from Example 1 in that, in Example 12, the complex microbial agent is the complex microbial agent prepared in Preparation Example 10.

[0059] Example 13

[0060] Example 13 differs from Example 1 in that, in Example 13, the mixed enzyme preparation includes cellulase and protease in a mass ratio of 2:0.5.

[0061] Example 14

[0062] Example 14 differs from Example 1 in that in Example 14, the mixed enzyme preparation comprises cellulase and protease in a mass ratio of 2:4.

[0063] Example 15

[0064] Example 15 differs from Example 1 in that in Example 15, the mixed enzyme preparation is added in an amount of 0.1wt% of the mixture.

[0065] Example 16

[0066] Example 16 differs from Example 1 in that in Example 16, the mixed enzyme preparation is added in an amount of 1.2wt% of the mixture.

[0067] Example 17

[0068] Example 17 differs from Example 1 in that in Example 17, the bio- bacterial fertilizer is further post-processed as follows: polyglutamic acid and chitosan are mixed in a mass ratio of 1:6 and dissolved in an 8wt% acetic acid solution, mixed at 70°C for 2h, forming an 8g / mL coating solution, and the bio-bacterial fertilizer is subjected to fluidized bed coating treatment with a coating thickness of 10μm.

[0069] Example 18

[0070] Example 18 differs from Example 1 in that in Example 17, the bio- bacterial fertilizer is further post-processed as follows: polyglutamic acid and chitosan are mixed in a mass ratio of 1:6 and dissolved in an 8wt% acetic acid solution, mixed at 70°C for 2h, forming a 10g / mL coating solution, and the bio-bacterial fertilizer is subjected to fluidized bed coating treatment with a coating thickness of 15μm. Comparative Example

[0071] Comparative Example 1 Comparative Example 1 differs from Example 1 in that in Comparative Example 1, no enzymatic treatment is performed. Detection Method

[0072] According to the preparation methods of Examples 1-18 and Comparative Examples 1-2, bio-bacterial fertilizers are obtained, and their performance indicators are determined according to NY-T525-2021 “Organic Fertilizer”, and the organic matter content, total nutrient content and seed germination number are recorded in Table 1.

[0073] Table 1 Performance indicators of bio-bacterial fertilizers Item Organic matter content / % Total nutrient content / % Seed germination / % Example 1 57.18 5.12 93.23 Example 2 58.35 5.21 94.32 Example 3 52.86 4.73 90.92 Example 4 53.01 4.68 91.12 Example 5 52.91 4.81 89.89 Example 6 52.81 4.69 90.21 Example 7 62.35 5.96 97.68 Example 8 63.15 6.12 97.92 Example 9 60.10 5.53 94.95 Example 10 60.06 5.50 95.12 Example 11 59.91 5.49 94.89 Example 12 60.03 5.51 94.91 Example 13 52.94 4.79 89.08 Example 14 53.05 4.81 88.76 Example 15 52.83 4.69 90.17 Example 16 53.91 4.73 90.06 Example 17 59.13 5.39 95.94 Example 18 59.06 5.41 96.07 Comparative Example 1 53.12 4.02 79.62 As can be seen from Examples 1-2, Comparative Example 1 and Table 1, the bio-fertilizer prepared in Examples 1-2 has high organic matter content, total nutrient content and seed germination number, indicating that the bio-fertilizer prepared in Examples 1-2 has high nutrient content and high fertilizer efficiency, and has better growth promotion effect on crops after application. In Examples 1-2, the raw materials are subjected to enzymatic treatment before fermentation, which can decompose cellulose, lignin and other difficult-to-ferment substances in the raw materials, thereby increasing the nutrient content and types of the raw materials before fermentation, and further improving the fermentation efficiency of organic matter and the fertilizer efficiency of the fermentation product. Through the synergistic effect of multiple strains in the composite microbial agent, the fermentation efficiency can be improved, the fermentation period can be shortened, and the activity of the fermentation product can be increased.

[0074] Compared with Examples 1-2, the organic matter content, total nutrient content and seed germination number of Examples 3-4 are all decreased. The addition amount of the composite microbial agent is changed in Examples 3-4, indicating that the addition amount of the composite microbial agent has an effect on the fermentation effect. When the addition amount of the composite microbial agent is reduced, the fermentation rate is reduced, and the product is not completely degraded. When the addition amount of the composite microbial agent is increased, the competition among the microbial populations is easy to occur, and the nutrients are insufficient, which affects the reproduction and expansion of the microbial populations.

[0075] Compared with Examples 1-2, the organic matter content, total nutrient content and seed germination number of Examples 5-6 are all decreased. The mass ratio of Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria in the microbial agent is changed in Examples 5-6, indicating that the mass ratio of Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria has an effect on the fermentation effect of the composite microbial agent. The four exist in a synergistic effect, which can promote the fermentation efficiency, improve the fermentation effect and the activity of the fermentation product, and shorten the fermentation period. At the same time, the growth of pathogenic bacteria can be effectively inhibited.

[0076] Compared with Examples 1-2, the organic matter content, total nutrient content and seed germination number of Examples 7-8 are increased. In Examples 7-8, the microbial agent is treated to form a microbial capsule. The capsule wall is formed by carboxymethyl starch sodium and sodium caseinate to coat and protect the microbial agent, so that the activity of the microbial agent can be maintained during the enzymatic treatment. The carboxymethyl starch sodium and sodium caseinate can be slowly decomposed by the enzyme during the enzymatic treatment. After the enzymatic treatment, the capsule wall is destroyed, and the microbial agent is released at the end of the enzymatic treatment, which provides a high-activity fermentation microbial agent for fermentation treatment.

[0077] Compared with examples 7-8, the organic matter content, total nutrient content and seed germination number of examples 9-12 are decreased, the mass ratio of sodium carboxymethyl starch and sodium caseinate is changed in examples 9-10, and the concentration of the homogeneous solution is changed in examples 11-12, which indicates that the mass ratio of sodium carboxymethyl starch and sodium caseinate and the concentration of the two affect the effect of the microbial agent microcapsule, reduce the protection effect of the microbial agent microcapsule on the microbial agent, and affect the release time of the microbial agent, so that the microbial agent is released too early or too late, is affected by enzyme hydrolysis or the fermentation progress is delayed, thereby reducing the effect of the fermentation product.

[0078] Compared with examples 1-2, the organic matter content, total nutrient content and seed germination number of examples 13-14 are decreased, and the mass ratio of cellulase and protease in the mixed enzyme preparation is changed in examples 13-14, which indicates that the protease can decompose the protein in the raw material, expose cellulose and lignin, etc. to the outside, which is beneficial to the enzyme hydrolysis effect and rate of cellulase, and the small molecule nutrient substances generated by enzyme hydrolysis are beneficial to the reproduction of the microbial population and provide a good foundation for enzyme hydrolysis treatment.

[0079] Compared with examples 1-2, the organic matter content, total nutrient content and seed germination number of examples 15-16 are decreased, and the addition amount of the mixed enzyme preparation is changed in examples 15-16, which indicates that the content of the mixed enzyme preparation affects the enzyme hydrolysis effect, and too little addition amount of the mixed enzyme preparation is prone to incomplete enzyme hydrolysis, and too much addition amount of the mixed enzyme preparation is prone to excessive enzyme hydrolysis, which is not conducive to maintaining the activity of the microbial agent.

[0080] Compared with examples 1-2, the organic matter content, total nutrient content and seed germination number of examples 17-18 are increased, and the biological bacterial fertilizer is coated in examples 17-18, which has good slow-release performance, can improve the storage stability and application effect of the fertilizer, and the coating formed by polyglutamic acid and chitosan has good antibacterial performance, which is beneficial to maintaining the activity of the biological bacterial fertilizer and reducing the pollution of the fertilizer.

[0081] The specific embodiments are only an explanation of the present application, and are not a limitation of the present application, and those skilled in the art can make modifications to the embodiments without creative contribution after reading the present specification, but as long as the modifications are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A method for preparing a bio-fertilizer, characterized in that: The method comprises the following steps: (1) mixing the agricultural and forestry wastes and the livestock and poultry manure according to the mass ratio of 3-5:1-1.2, adjusting the moisture content to 50-60% after primary screening; (2) adding a compound microbial agent, and obtaining a mixture after uniform mixing; (3) performing enzymatic treatment and dynamic fermentation on the mixture to obtain a coarse granular fertilizer; (4) performing crushing, granulation and screening on the coarse granular fertilizer to obtain a biological bacterial fertilizer.

2. The method according to claim 1, characterized in that: The compound microbial agent comprises a microbial agent and bran in a mass ratio of 1:9.5-10.5, the microbial agent comprises Bacillus subtilis, actinomycetes, Trichoderma and photosynthetic bacteria in a mass ratio of 3-4:2-3:1.5-2.5:1-1.5, and the addition amount of the compound microbial agent is 0.5-1wt% of the mixture.

3. The method according to claim 1, characterized in that: The enzymatic treatment comprises the following steps: adding a mixed enzyme preparation at 45-55℃, and performing constant-temperature enzymolysis for 24-48h at pH 6-7.5, and the addition amount of the mixed enzyme preparation is 0.3-0.8wt% of the mixture.

4. The method according to claim 3, characterized in that: The mixed enzyme preparation comprises cellulase and protease in a mass ratio of 2-5:1-3.

5. The method for preparing a bio-fertilizer according to claim 1, characterized in that: The dynamic fermentation comprises the following steps: turning over once every 8h, maintaining the temperature at 30-40℃, and continuing for 7-10d until the compost is rotted.

6. The method for preparing a bio-fertilizer according to claim 2, characterized in that: The microbial agent is further treated as follows: adding the microbial agent into water and uniformly dispersing to obtain a microbial liquid for standby, dissolving carboxymethyl sodium starch and sodium caseinate in water, uniformly mixing, and then standing at 4℃ for 24h to obtain a homogeneous solution, and then adding the microbial liquid drop by drop while stirring, uniformly mixing, and then freeze-drying to obtain a microbial agent microcapsule.

7. The method for preparing a bio-fertilizer according to claim 6, characterized in that: The mass ratio of the carboxymethyl sodium starch and the sodium caseinate is 0.9-1.1:5.6-5.9, and the concentration of the homogeneous solution is 1.5-2g / mL.

8. The method for preparing a bio-fertilizer according to claim 1, characterized in that: The biological bacterial fertilizer is further post-treated as follows: mixing polyglutamic acid and chitosan in an acetic acid solution and mixing at 70℃ for 2h to form a coating solution, and then performing fluidized bed coating treatment on the biological bacterial fertilizer, and the coating thickness is 10-15μm.