Jujube polysaccharide nano-selenium as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of food processing, and particularly relates to jujube polysaccharide nano-selenium as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing a jujube polysaccharide solution with a Na2SeO3 solution, then adding a Vc solution, stirring until an orange red solution is formed, dialyzing, and freeze-drying to obtain the jujube polysaccharide nano-selenium. The jujube polysaccharide adopted in the preparation process is used as a polysaccharide substance and has rich functional groups; according to the method, Chinese date polysaccharide serves as a stabilizer, Vc is utilized for reducing sodium selenite, the Chinese date polysaccharide nano-selenium is prepared, and the Chinese date polysaccharide nano-selenium prepared through the preparation method can reduce the content of TNF-alpha and IL-1beta in colon tissue and the content of LBP in serum and repair intestinal barriers and has a good anti-inflammatory effect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of food processing, and particularly relates to a jujube polysaccharide nano selenium as well as a preparation method and application thereof. BACKGROUND

[0002] Selenium (Se) is an essential trace element for human body, participates in various physiological activities, and plays an important role in delaying aging, improving immunity, preventing cardiovascular diseases, treating diabetes and resisting tumors. Human body can only obtain daily selenium requirement through food intake. However, the difference between the intake amount of selenium and the toxic dose is small, and when exceeding a certain safety limit, selenium will have toxic effects on human body. Therefore, how to provide a safe and effective selenium supplement method has become a difficult problem.

[0003] Nano selenium is a new source of selenium, with protein as the core and selenium as the membrane. Nano selenium has high water solubility, low toxicity and strong catalytic activity, and is more easily absorbed and transformed than inorganic or organic selenium. As a kind of elemental selenium, selenium nanoparticles (SeNPs) have high biological activity and bioavailability, low toxicity, and good application prospects in antioxidant, antitumor, diabetes treatment and antiobesity. However, due to high surface energy, SeNPs are usually unstable in aqueous solution, which seriously limits the actual application. SUMMARY

[0004] The purpose of the present application is to solve the problems of the prior art, and to provide a jujube polysaccharide nano selenium as well as a preparation method and application thereof. The following technical solutions are specifically used: In a first aspect, the present application provides a preparation method of jujube polysaccharide nano selenium, comprising the following steps: Mixing jujube polysaccharide solution and Na2SeO3 solution, then adding Vc solution, stirring until an orange-red solution is formed, dialysis, freeze-drying to obtain the jujube polysaccharide nano selenium.

[0005] As a further preferred embodiment, the jujube polysaccharide is prepared by the following steps: Drying and crushing jujube, and adding ethanol for soaking, drying after soaking, then adding water, and performing water extraction under the condition of water bath, centrifugation to obtain supernatant; Concentrating the supernatant, adding ethanol for alcohol precipitation, standing, centrifugation to obtain crude polysaccharide; Resolving the crude polysaccharide, then adding Sevag reagent to remove protein, shaking, centrifugation, concentration, freeze-drying to obtain the jujube polysaccharide.

[0006] As a further preferred embodiment, the ratio of jujube to water is 1:20.

[0007] As a further preferred embodiment, the temperature of the water bath is 90-100 DEG C, and the time of the water bath is 1.5-3 hours.

[0008] As a further preferred embodiment, the volume ratio of the crude polysaccharide to the Sevag reagent is 4:1.

[0009] As a further preferred embodiment, the concentration of the jujube polysaccharide solution is 0.6-3.0 mg / mL.

[0010] Since SeNPs are unstable, easy to aggregate and transform into non-biological active or gray selenium element with crystal structure, the present application uses Vc solution to reduce SeO3 2‒ to Se by using chemical reduction method, and by changing the polysaccharide concentration, the particle size and ZETA potential of the complex are changed, thereby affecting the stability.

[0011] As a further preferred embodiment, the volume ratio of the jujube polysaccharide solution, the Na2SeO3 solution and the Vc solution is 1:4:4.

[0012] In the second aspect, the present application provides a jujube polysaccharide nano selenium prepared by the above preparation method.

[0013] In the third aspect, the present application further provides the use of the above jujube polysaccharide nano selenium in preparing an anti-inflammatory product.

[0014] As a further preferred embodiment, the anti-inflammatory product can be used to reduce the content of TNF-alpha and IL-1 beta in colon tissue, reduce the content of LBP in serum and repair intestinal barrier.

[0015] The beneficial effects of the present application are: The jujube polysaccharide used in the preparation process of the present application has abundant functional groups as a polysaccharide substance; the method uses jujube polysaccharide as a stabilizer, reduces sodium selenite by using Vc, and prepares jujube polysaccharide nano selenium; the jujube polysaccharide nano selenium prepared by the preparation method of the present application can reduce the content of TNF-alpha and IL-1 beta in colon tissue and the content of LBP in serum, and can repair intestinal barrier, and has good anti-inflammatory effect. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings used in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0017] Figure 1The figure shows the determination results of the particle size of Jujube polysaccharide selenium nanoparticles prepared by different concentrations of Jujube polysaccharide solution; Figure 2 The figure shows the infrared spectrum of JP and JP-SeNPs prepared in Example 2; Figure 3 The figure shows the scanning electron microscope images of JP (A) and JP-SeNPs (B) prepared in Example 2; Figure 4 The figure shows the X-ray diffraction pattern of JP-SeNPs prepared in Example 2; Figure 5 The figure shows the X-ray photoelectron spectrogram of JP-SeNPs prepared in Example 2, where A and B are the full spectrum and selenium spectrum, respectively; Figure 6 The figure shows the effect of pH value on the stability of JP-SeNPs prepared in Example 2; Figure 7 The figure shows the effect of temperature on the storage stability of JP-SeNPs prepared in Example 2 Figure 8 The figure shows the effect of JP-SeNPs prepared in Example 2 on the TNF-α content of DSS-induced C57BL / 6 mice; Figure 9 The figure shows the effect of JP-SeNPs prepared in Example 2 on the IL-1β content of DSS-induced C57BL / 6 mice; Figure 10 The figure shows the effect of JP-SeNPs prepared in Example 2 on the LBP content of DSS-induced C57BL / 6 mice.

[0018] Figure 11 The figure shows the effect of JP-SeNPs prepared in Example 2 on the tight junction protein content of DSS-induced C57BL / 6 mice. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0020] The reagents and main instruments used in the following embodiments are as follows: Main reagents such as sodium selenite (Shanghai Aladdin Bio-Chem Technology Co., Ltd.), Vc (Shanghai Aladdin Bio-Chem Technology Co., Ltd.) Main instruments: nano force potential instrument (Malvern PANalytical), magnetic stirrer (Guohua (Changzhou) Instrument Manufacturing Co., Ltd.) Example 1 A preparation method of jujube polysaccharide nano selenium, and the specific preparation process is as follows: (1) Extraction of jujube polysaccharide After drying the jujube at 60℃ for 4h, it was crushed and passed through a 40 mesh sieve. 95% ethanol was added overnight at a solid-liquid ratio of 1:5 (g / mL). After drying, the liquid-solid ratio was 1:20 (g / mL) and the water bath was 95℃ for 2h. The extraction was repeated twice, centrifuged, and the supernatant was concentrated to 1 / 10 of the volume. Anhydrous ethanol was added to precipitate the polysaccharide, which was placed in the refrigerator at 4℃ overnight and centrifuged to obtain crude polysaccharide.

[0021] The obtained crude polysaccharide was redissolved and the protein was removed by Sevag method. The volume ratio of sample to Sevag reagent was 4:1, shaken, centrifuged, and the supernatant was collected. The process was repeated 5 times, and the supernatant was concentrated and freeze-dried to obtain jujube polysaccharide (JP).

[0022] (2) Preparation of jujube polysaccharide nano selenium The jujube polysaccharide was dissolved in water to prepare a jujube polysaccharide solution with a concentration of 0.6 mg / mL. The solution was mixed with 0.01 mol / L Na2SeO3 solution at a volume ratio of 1:4, stirred at room temperature with a magnetic stirrer for 30 min, and then 0.04 mol / L Vc solution was added in an equal volume of Na2SeO3 solution. Stirring was carried out at 35℃ for 6h until a stable orange-red solution was formed. The solution was dialyzed at 4℃ for 48h and freeze-dried to obtain jujube polysaccharide nano selenium (JP-SeNPs).

[0023] Example 2 A preparation method of jujube polysaccharide nano selenium, and the specific preparation process is similar to that of Example 1, the only difference is that "preparing a jujube polysaccharide solution with a concentration of 0.6 mg / mL" is replaced by "preparing a jujube polysaccharide solution with a concentration of 0.8 mg / mL", and the rest of the process remains unchanged, jujube polysaccharide nano selenium is prepared.

[0024] Example 3 A preparation method of jujube polysaccharide nano selenium, and the specific preparation process is similar to that of Example 1, the only difference is that "preparing a jujube polysaccharide solution with a concentration of 0.6 mg / mL" is replaced by "preparing a jujube polysaccharide solution with a concentration of 1.0 mg / mL", and the rest of the process remains unchanged, jujube polysaccharide nano selenium is prepared.

[0025] Example 4 A preparation method of jujube polysaccharide nano selenium, the specific preparation process is similar to that of example 1, the only difference is that the "preparation of jujube polysaccharide solution with a concentration of 0.6 mg / mL" is replaced by "preparation of jujube polysaccharide solution with a concentration of 2.0 mg / mL", the rest is unchanged, jujube polysaccharide nano selenium is prepared.

[0026] Example 5 A preparation method of jujube polysaccharide nano selenium, the specific preparation process is similar to that of example 1, the only difference is that the "preparation of jujube polysaccharide solution with a concentration of 0.6 mg / mL" is replaced by "preparation of jujube polysaccharide solution with a concentration of 3.0 mg / mL", the rest is unchanged, jujube polysaccharide nano selenium is prepared.

[0027] Example 6 The jujube polysaccharide nano selenium prepared above is subjected to performance characterization test, specifically as follows: GraphPad Prism software is used for analysis, P<0.05 represents significant difference.

[0028] (1) Particle size and zeta potential determination Particle size and zeta potential are important indicators affecting the stability of nanoparticles. Large particle size of nanoparticles will cause aggregation on one hand, and on the other hand, it will be greatly affected by gravity, with accelerated sedimentation speed and poor stability. The greater the absolute value of zeta potential, the more stable it is. Malvern nanoparticle size potential instrument is used to detect the particle size and potential of the sample. The specific process is as follows: take an appropriate amount of dialyzed reaction liquid and place it in a sample cell, and detect under the conditions of detection temperature 25℃ and equilibrium time 120s.

[0029] The detection results are shown in Figure 1 When the concentration of jujube polysaccharide solution is 0.8 mg / mL, the particle size of JP-SeNPs is 142 nm, and the zeta potential is-26.66 mV. It shows that when the concentration of jujube polysaccharide solution is 0.8 mg / mL, jujube polysaccharide nano selenium is the most stable.

[0030] (2) Infrared spectrum determination Take 1-2 mg of jujube polysaccharide and jujube polysaccharide nano selenium sample and mix with potassium bromide powder, then grind into powder. Use solid tabletting method to scan by Fourier infrared spectrometer, and set the scanning range to 400-4000 cm -1 , resolution is 4cm -1 .

[0031] The results are shown in Figure 2As shown, the absorption bands observed in FT-IR spectra of JP and JP-SeNPs were almost the same, which indicated that the skeleton structure of JP was kept unchanged after the formation of SeNPs. Specifically, the absorption peak of JP at 3417.4 cm -1 was the characteristic peak of O-H of polysaccharide, while the O-H characteristic absorption peak of JP-SeNPs was shifted to lower wavelength 3383.1 cm -1 . This red shift phenomenon indicated that SeNPs might interact with O-H group and then form Se-O bond.

[0032] (3) Scanning electron microscopy determination The sample to be tested was fixed on the sample stage with conductive glue, and the surface was sprayed with gold to enhance the electrical conductivity. The acceleration voltage and working distance were adjusted to the appropriate parameters, and the surface of the sample was photographed at different magnifications to observe its morphological characteristics.

[0033] The results are shown in Figure 3 , and it can be seen from Figure 3 A that JP showed a loose flaky structure on the surface, while SeNPs modified by JP showed single complete and well dispersed spherical nanoparticles as shown in Figure 3 B, which indicated that JP could effectively inhibit the aggregation of SeNPs.

[0034] (4) X-ray diffraction analysis An appropriate amount of sample was taken on a glass sample carrier, crushed and compacted, placed on the sample stage, Cu Kα radiation, tube voltage 40 kV, tube current 30 mA, scanning range 10°-90°, scanning rate 4 ° / min.

[0035] The results are shown in Figure 4 , according to the standard spectrum analysis of selenium, there are two sharp characteristic peaks at 2θ of 24° and 30°. However, from the experimental results, it can be seen that JP-SeNPs only showed two broadened diffraction peaks, which were similar to the diffraction characteristics of polysaccharide, and were significantly different from the sharp Bragg diffraction peaks in the selenium standard card. Therefore, the results of XRD spectrum showed that when jujube polysaccharide was used as a template to stabilize nanoseelenium, selenium did not form a crystal structure, but was in an amorphous state of amorphous form.

[0036] (5) X-ray photoelectron spectroscopy analysis An appropriate amount of nanoseelenium lyophilized powder was determined. The analysis conditions were: the vacuum degree of the analysis chamber was 5x10 -9torr, X-ray source is monochromatic Al Kα source (Mono AlKα), energy 1486.6 eV, 5 mA x 15 kV, beam spot size 700 x 300 μm, scanning mode CAE. Full spectrum scan: pass energy 160 eV; narrow spectrum scan: pass energy 40 eV. Without special instructions, the number of scans is 1. The resulting spectra are finally calibrated with the C1s at 284.6 eV.

[0037] The results are shown in Figure 5 A, as Figure 5 can be seen, three characteristic peaks of C1s, O1s and Se3d are detected in the full spectrum, and the Se3d peak corresponds to zero-valent selenium. As Figure 5 B can be seen, by peak fitting the spectrum in the range of 50-62 eV, it is found that the two fitting peaks of Se3d are located at 53.50 eV and 54.45 eV. By comparison with NIST X-ray photoelectron spectroscopy database, it is known that the two peaks are both the electron binding energy peaks of zero-valent selenium, and the 59.1 eV electron binding energy peak corresponding to sodium selenite is not observed. This indicates that the reaction is complete, and the selenium in JP-SeNPs exists in the form of zero-valent, which is consistent with the analysis of the full spectrum.

[0038] Example 7 Analysis of the stability of Jujube polysaccharide selenium nanoparticles under different factors (1) Explore the effect of pH on the stability of JP-SeNPs By adding 0.01 mol / L of HCL and 0.01 mol / L of NaOH to adjust the solution, jujube polysaccharide selenium nanoparticle solutions with different pH (2-10) were prepared, and the particle size was measured by a nanoparticle size potential instrument after mixing.

[0039] The results are shown in Figure 6 , the pH value is relatively stable in the range of 4-10 with a smaller change range. When the pH value is 2, the particle size is about 600 nm, at this time JP-SeNPs is unstable, because the electrochemical properties of SeNPs surface have changed, new chemical bonds may be produced, further affecting the stability. In food production, processing, storage and other aspects, the pH value of food is changing all the time. Therefore, it is necessary to evaluate the pH stability of nanomaterials for predicting food applications.

[0040] (2) Explore the effect of storage temperature on the stability of JP-SeNPs Jujube polysaccharide selenium nanoparticles were stored at 4 ℃ and room temperature for 37 days, respectively, and the particle size was measured by a nanoparticle size potential instrument.

[0041] The change of particle size of JP-SeNPs during storage is shown in Figure 7As shown, it can be concluded that the optimal storage temperature of JP-SeNPs is 4 ℃.

[0042] The present application uses jujube polysaccharide solution with different concentrations as a stabilizer to prepare jujube polysaccharide selenium nanoparticles, and the jujube polysaccharide selenium nanoparticles are characterized and the stability is studied. The results show that when the concentration of jujube polysaccharide solution is 0.8 mg / mL, the jujube polysaccharide selenium nanoparticles are most stable, the particle size is 142 nm, and the zeta potential is-26.66 mV. By FT-IR, it can be concluded that the OH group in JP interacts with the Se atom in SeNPs through a hydrogen bond-like interaction. By SEM, it is found that JP-SeNPs are spherical particles, which are formed under the coating of JP. By XRD, it is known that when jujube polysaccharide is used as a template to stabilize selenium nanoparticles, selenium does not form a crystal structure, but is in an amorphous state. According to XPS, the selenium in JP-SeNPs is zero-valent selenium. According to the stability experiment, under strong acid conditions, JP-SeNPs may form new chemical bonds, thereby further affecting its stability. According to the storage stability, the storage stability is best at 4 ℃, and the above results lay a foundation for further research on jujube polysaccharide selenium nanoparticles.

[0043] Example 8 Anti-inflammatory activity of jujube polysaccharide selenium nanoparticles Under the stimulation of DSS, C57BL / 6 mice were modeled for acute colitis, and the expression of inflammatory factors in the colon tissue was detected to determine the related mechanism of inflammation. In this embodiment, a DSS-induced C57BL / 6 mouse acute colitis model was established, and the expression of related inflammatory factors in C57BL / 6 mice under the stimulation of DSS was explored to evaluate the biological activity of JP and JP-SeNPs.

[0044] Main reagents: dextran sulfate sodium (MP Biomedicals), PBS buffer (Seville Biotechnology), ELISA kit (Shanghai Enzyme-linked Biotechnology Co., Ltd.).

[0045] Experimental method Animal experiment design C57BL / 6 mice (about 20 grams in weight, 6 weeks old, male). The temperature of the mouse growth environment is 25±0.5 ℃, the humidity is 50-60%, and the light cycle is 12 h. The mouse model of ulcerative colitis was established by DSS induction.

[0046] The mice were randomly divided into 4 groups (n=6), including: normal group (labeled as group A): throughout the normal drinking water, and at the same time, normal saline was given by gavage for 14 days. Colitis model group (labeled as group B): DSS-containing drinking water was given for 7 days, and normal saline was given by gavage for 14 days. Colitis + JP group (labeled as group C), colitis + JP-SeNPs group (labeled as group D): DSS-containing drinking water was given for 7 days, and JP and JP-SeNPs at 3 mg / kg and 15 mg / kg, respectively, were given by gavage to the mice for 14 days.

[0047] Effects of Jujuboside and Jujuboside Nano Selenium on Tight Junction Protein in Colon Tissue, Secretion Level of Inflammatory Factors, and Lipopolysaccharide Binding Protein (LBP) Content in Serum. Control group, model group, jujuboside group, and jujuboside nano selenium group were set up, and the instructions in the test kit were used for determination.

[0048] Data processing GraphPad Prism software was used for analysis, and P<0.05 represented significant difference.

[0049] Results and analysis 1. Effects of Jujuboside and Jujuboside Nano Selenium on Secretion of Inflammatory Factors in DSS-induced Acute Colitis in C57BL / 6 Mice 1) Effects of JP and JP-SeNPs on TNF-α content Tumor necrosis factor (TNF-α) is a cytokine produced by various immune or non-immune cells, which can release IL-6 and IL-1β, and is one of the strongest inflammatory mediators. Abnormal TNF-α can cause many diseases, such as cancer, rheumatoid arthritis, Crohn's disease, etc.

[0050] The results are shown in Figure 8 Compared with the blank group, the content of TNF-α in the model group was significantly increased. After treatment with JP and JP-SeNPs under DSS stimulation, the content of TNF-α decreased to different degrees.

[0051] 2) Effects of JP and JP-SeNPs on IL-1β content IL-1β is a pro-inflammatory factor that can bind to immune cell surface receptors to activate NF-κB and MAPK signaling pathways.

[0052] The results are shown in Figure 9 Compared with the blank group, the content of IL-1β in the model group was significantly increased. After treatment with JP and JP-SeNPs under DSS stimulation, the content of IL-1β decreased to different degrees.

[0053] 2. Effect of Jujube Polysaccharide and Jujube Polysaccharide Nano Selenium on the Content of Lipopolysaccharide Binding Protein (LBP) in Serum of DSS-induced C57BL / 6 Mice with Acute Colitis 1) Effect of JP and JP-SeNPs on the content of LBP LBP binds to CD14 receptors, activates immune cells such as monocytes, endothelial cells, and releases inflammatory mediators such as IL-1, IL-6 and TNF-α. Excessive activation may trigger systemic inflammatory response syndrome. From Figure 10 As shown in FIG. 4, compared with the blank group, the content of LBP in the model group was significantly increased. After DSS stimulation, the content of LBP was decreased to different degrees after treatment with JP and JP-SeNPs.

[0054] 3. Effect of Jujube Polysaccharide and Jujube Polysaccharide Nano Selenium on Tight Junction Proteins (ZO-1 and Occludin) in Colon Tissue of DSS-induced C57BL / 6 Mice with Acute Colitis 1) Effect of JP and JP-SeNPs on the content of ZO-1 ZO-1 protein is one of these tight junction proteins. ZO-1 protein not only participates in maintaining the mechanical barrier and permeability of mucosal epithelium, but also is involved in regulating cell material transport, maintaining epithelial polarity, and is related to information transmission and regulation of cell proliferation and differentiation, tumor cell metastasis and gene transcription. From Figure 11 As shown in FIG. 5, compared with the blank group, the fluorescence intensity of ZO-1 protein in the model group was significantly weakened. After DSS stimulation, the fluorescence intensity of ZO-1 protein was increased to different degrees after treatment with JP and JP-SeNPs.

[0055] 2) Effect of JP and JP-SeNPs on the content of Occludin Occludin, as a structural protein, participates in regulating cell permeability in cell tight junction and constitutes tight junction complex, and plays a key role in maintaining epithelial permeability and mucosal barrier function. From Figure 11 As shown in FIG. 6, compared with the blank group, the fluorescence intensity of Occuidin protein in the model group was significantly weakened. After DSS stimulation, the fluorescence intensity of Occludin protein was increased to different degrees after treatment with JP and JP-SeNPs.

[0056] The present application found that JP and JP-SeNPs have a protective effect on C57BL / 6 mouse model of colitis, can reduce the content of TNF-α and IL-1β in colon tissue, the content of LBP in serum and repair the intestinal barrier.

[0057] The embodiments of the present application are described above with reference to the drawings, and the principles and implementation manners of the present application are described herein by applying specific examples, and the above descriptions of the embodiments are only used to help understand the core ideas of the present application, but the present application is not limited to the specific implementation manners described above, and the specific implementation manners described above are only illustrative rather than limiting, and those of ordinary skill in the art can make many forms under the inspiration of the present application without departing from the scope of the present application and the protection scope of the claims, and all of them belong to the protection of the present application.

Claims

1. A method for preparing jujube polysaccharide nano-selenium, characterized in that, Includes the following steps: The jujube polysaccharide solution was mixed with Na2SeO3 solution, then vitamin C solution was added and stirred until an orange-red solution was formed. The solution was dialyzed and freeze-dried to obtain the jujube polysaccharide nano-selenium.

2. The preparation method according to claim 1, characterized in that, The jujube polysaccharide was prepared by the following steps: The jujubes were dried and crushed, then soaked in ethanol. After soaking, they were dried again, then water was added, and water extraction was performed under water bath conditions. After centrifugation, the supernatant was obtained. The supernatant was concentrated, ethanol was added for alcohol precipitation, and the mixture was allowed to stand and centrifuged to obtain crude polysaccharide. The crude polysaccharide was reconstituted, then Sevag reagent was added to remove proteins, followed by shaking, centrifugation, concentration, and freeze-drying to obtain the jujube polysaccharide.

3. The preparation method according to claim 2, characterized in that, The ratio of jujubes to water is 1:

20.

4. The preparation method according to claim 2, characterized in that, The water bath temperature is 90℃-100℃, and the water bath time is 1.5h-3h.

5. The preparation method according to claim 2, characterized in that, The volume ratio of the crude polysaccharide to the Sevag reagent is 4:

1.

6. The preparation method according to claim 1, characterized in that, The concentration of the jujube polysaccharide solution is 0.6 mg / mL to 3.0 mg / mL.

7. The preparation method according to claim 6, characterized in that, The volume ratio of the jujube polysaccharide solution, Na2SeO3 solution, and Vc solution is 1:4:

4.

8. A jujube polysaccharide nano-selenium, characterized in that, It is prepared by the preparation method according to any one of claims 1-7.

9. The application of the jujube polysaccharide nano-selenium according to claim 8 in the preparation of anti-inflammatory products.

10. The application according to claim 9, characterized in that, The anti-inflammatory product can be used to reduce the levels of TNF-α and IL-1β in colon tissue, reduce the level of LBP in serum, and repair the intestinal barrier.

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