Near-infrared fluorescence immunoassay analyzer

Abstract

The invention discloses a near-infrared fluorescence immunoassay analyzer, and relates to the field of biomedical engineering.The near-infrared fluorescence immunoassay analyzer comprises an analyzer body, a dome-type fluorescence collector is installed in an inner cavity of a sealing seat, a narrow-band light filter is installed at the bottom of the dome-type fluorescence collector, and a piezoelectric focusing lens set is installed at the bottom of a light source sliding block; a light source sliding block is arranged in the analyzer body, a laser range finder and an optical fiber beam splitter are respectively arranged on two sides of the light source sliding block, a double-layer reagent card base body is arranged in the center of the top of the analyzer body, a snakelike microfluidic channel is formed in the top of the double-layer reagent card base body, a shape memory alloy is arranged at the bottom of the double-layer reagent card base body, and a thermosensitive actuator array is arranged at the bottom of the shape memory alloy. According to the near-infrared fluorescence immunoassay analyzer, eight-path parallel scanning is combined with dynamic focusing, so that the single-sample full-index detection time can be greatly shortened, the near-infrared fluorescence immunoassay analyzer can be compatible with various complex biological samples, a chemical color developing agent is not needed, and the experiment cost and the environmental pollution risk are reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biomedical engineering, in particular to a near-infrared fluorescence immunoassay analyzer. BACKGROUND

[0002] Antigen-antibody specific binding reaction is the core principle of disease diagnosis, which is widely used in infectious disease screening, tumor marker detection and other fields. The current mainstream technologies include enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay, etc., which all need to rely on solid carriers to complete the formation of immune complexes and signal conversion.

[0003] In the field of immunoassay, the traditional methods such as enzyme-linked immunosorbent assay or chemiluminescence detection have the following inherent defects: Complex sample pretreatment: multiple steps of chemical treatment such as labeling, washing and color development are required for the sample, which is tedious and easy to introduce human error; limited sensitivity: visible light or ultraviolet band detection is easily disturbed by sample matrix such as serum background fluorescence and particulate matter scattering, making it difficult to detect weak signal targets; dynamic response lag: fixed light path cannot adapt to the deformation of microfluidic chip or the change of liquid refractive index, resulting in focus drift and signal attenuation; single-point detection efficiency is low: point-by-point scanning mode is time-consuming and difficult to meet the demand of high-throughput screening; high reagent dependence: relying on enzyme reaction or chemical substrate, there are batch differences, environmental pollution and storage stability problems, and it is also inconvenient to measure and analyze some new materials.

[0004] Therefore, it is necessary to provide a near-infrared fluorescence immunoassay analyzer to solve the above problems. SUMMARY

[0005] The main purpose of the present application is to provide a near-infrared fluorescence immunoassay analyzer, which can effectively solve the problems in the background art.

[0006] To achieve the above purpose, the technical scheme adopted by the present application is: A near-infrared fluorescence immunoassay analyzer, comprising an analyzer body, a double-layer reagent card substrate is installed in the middle of the top of the analyzer body, a serpentine microfluidic channel is formed in the top of the double-layer reagent card substrate, a shape memory alloy is installed at the bottom of the double-layer reagent card substrate, a thermal actuator array is installed at the bottom of the shape memory alloy, and the inner wall of the serpentine microfluidic channel is coated with an antibody; A sealing seat is installed on the top of the analyzer body, a dome-shaped fluorescence collector is installed in the inner cavity of the sealing seat, a narrow-band filter is installed at the bottom of the dome-shaped fluorescence collector, an arc-shaped slide rail is rotatably connected in the inner cavity of the sealing seat, and a light source sliding block is installed on the outer wall of the arc-shaped slide rail; A rotating ring is installed at the bottom of the arc-shaped slide rail, a gear ring is installed at the bottom of the rotating ring, a second gear is meshed on the side of the gear ring, a worm gear is provided at the bottom of the second gear, a connecting shaft is rotatably connected in the inner cavity of the analyzer body, a sleeve is sleeved on the outer wall of the connecting shaft, a lifting assembly is attached to the bottom of the double-layer reagent card base, limit frames are symmetrically arranged on both sides of the lifting assembly, and a support frame is provided on the opposite side of the two limit frames.

[0007] Preferably, a piezoelectric focusing lens group is installed at the bottom of the light source slider, a laser rangefinder and an optical fiber beam splitter are respectively installed on both sides of the light source slider, a feeding conveyor belt is installed in the inner cavity of the analyzer body, the feeding conveyor belt extends to the top of one end of the support frame, the feeding conveyor belt is located at the top of the adapter rod, and indium gallium arsenide photoelectric sensors are symmetrically installed on both sides of the bottom of the dome-shaped fluorescence collector.

[0008] Preferably, a laser diode is installed at the bottom of the inner cavity of the light source slider, the output end of the laser diode is located at the top of the piezoelectric focusing lens group, a self-cleaning lens is installed at the bottom of the fiber beam splitter, a miniature air pump is installed on the side of the bottom of the self-cleaning lens, and the output end of the laser diode is also connected to a single-mode fiber through an FC / APC fiber optic connector to transmit the laser light source emitted by it to the fiber beam splitter. The fiber beam splitter is used to split the laser light source emitted by the laser diode into eight independent beams.

[0009] Preferably, reflection-enhancing prisms are symmetrically installed on both sides of the inner cavity of the serpentine microfluidic channel. The reflection-enhancing prisms are positioned at a 45-degree angle within the inner cavity of the serpentine microfluidic channel. The reflection-enhancing prisms are used to reflect the lateral scattered light source in the inner cavity of the serpentine microfluidic channel to the dome-shaped fluorescence collector. The reflection-enhancing prisms are higher than the serpentine microfluidic channel. A transparent PDMS layer is installed at the bottom of the inner cavity of the serpentine microfluidic channel. The antibody in the serpentine microfluidic channel is a streptavidin-biotin complex, used to bind quantum dot-labeled detection antibodies.

[0010] Preferably, the thermal actuator array comprises sixty-four units arranged in a grid pattern to cover the bottom of the double-layer reagent card substrate. The thermal actuator array contacts the shape memory alloy through the contacts of the units. The double-layer reagent card substrate is a flexible substrate.

[0011] Preferably, a main shaft is installed in the center of the second gear, a worm gear is installed on the outer wall of the main shaft, a stepper motor is installed at the bottom of the inner cavity of the analyzer body, the stepper motor is connected to the main shaft through a coupling, one end of the connecting shaft is rotatably connected in the inner cavity of the analyzer body, and a worm is installed at the other end of the connecting shaft, the worm meshing with the worm gear.

[0012] Preferably, an electric push rod is installed at the center of the bottom of the sleeve. The top of the electric push rod penetrates the outer wall of the sleeve and fits against the outer wall of the connecting shaft. Fixed posts are symmetrically installed at both ends of the top of the sleeve. A second guide rod is installed in the inner cavity of the fixed post. A second spring is wound around the outer wall of the second guide rod. Adaptor rods are symmetrically and movably connected to the bottom of both ends of the lifting assembly. The end of the adapter rod near the fixed post is movably connected to the inner cavity of the fixed post and sleeved on the outer wall of the second guide rod. The top of the second spring is connected to the adapter rod, and the bottom of the second spring is installed at the bottom of the inner cavity of the fixed post.

[0013] Preferably, limiting blocks are symmetrically installed around the top of the analyzer body cavity, the bottom of the limiting blocks are attached to the top of the double-layer reagent card base, the top of the support frame is attached to the bottom of the double-layer reagent card base, and a linear motor is installed on the side of the limiting frame near the support frame, and the output end of the linear motor is connected to the support frame.

[0014] Preferably, a first guide rod is symmetrically installed at the bottom of the inner cavity at both ends of the lifting assembly. A first spring is wound around the outer wall of the first guide rod. The other end of the adapter rod is movably connected to the inner cavity of the lifting assembly and sleeved on the outer wall of the first guide rod. The top of the first spring is connected to the adapter rod, and the bottom of the first spring is installed at the bottom of the inner cavity of the lifting assembly. The tops of both sides of the lifting assembly are inclined, and the bottoms of the opposite sides of the two limiting frames are also inclined.

[0015] Preferably, a feedback stepper motor is installed in the inner cavity of the analyzer body, and a first gear is mounted on the feedback stepper motor through a first rotating shaft. A rack is movably connected in the inner cavity of the analyzer body, and the rack meshes with the first gear. The first gear is located at the bottom of the lifting assembly.

[0016] Compared with the prior art, the present invention provides a near-infrared fluorescence immunoassay analyzer, which has the following beneficial effects: This near-infrared fluorescence immunoassay analyzer uses a fiber optic beam splitter to divide a single laser beam into eight independent beams, which are simultaneously projected onto different areas of the dual-layer reagent card substrate through a self-cleaning lens. This allows for a single scan to cover eight times the detection area, significantly shortening the full sample detection time. The piezoelectric focusing lens group can adjust the focus in real time to compensate for deformation of the dual-layer reagent card substrate or changes in liquid refraction, ensuring that the laser focus is always accurately located at the reaction interface, thus effectively avoiding signal defocusing loss.

[0017] This near-infrared fluorescence immunoassay analyzer features a lifting assembly and rack that can lift the center of the double-layer reagent card substrate to form a parabolic bulge. Based on this, it can actively match the curvature of the laser scanning, improving fluorescence collection efficiency. Through the set thermal actuator array, it can drive the shape memory alloy to micro-shrink, locally correcting residual deformation errors and ensuring signal consistency across the entire area. The reflection enhancement prism can perform directional light guidance, efficiently reflecting laterally scattered fluorescence to the dome-shaped fluorescence collector. Therefore, it can overcome the defect of insufficient penetration depth of near-infrared light, resulting in a significant improvement in signal-to-noise ratio. Through the set serpentine microfluidic channel, it can accelerate reagent mixing through the Dean vortex effect, reduce the accumulation of reaction residues, and reduce background noise caused by fluid heterogeneity.

[0018] This near-infrared fluorescence immunoassay analyzer uses a narrow-band filter that transmits only the target fluorescence wavelength, filtering out ambient stray light and residual excitation light. With its indium gallium arsenide photoelectric sensor, it can capture near-infrared signals with high sensitivity, avoiding visible light background interference.

[0019] This near-infrared fluorescence immunoassay analyzer features a non-invasive reaction monitoring system. It utilizes near-infrared laser excitation for deep penetration, directly exciting fluorescent markers within microchannels through polydimethylsiloxane. This eliminates the need for opening the sample container or adding substrates, avoiding aerosol contamination and cross-contamination. A miniature air pump allows for air-blowing cleaning, preventing light path attenuation caused by droplet adhesion or dust obstruction, thus ensuring long-term detection stability.

[0020] This near-infrared fluorescence immunoassay analyzer uses an indium gallium arsenide photoelectric sensor to monitor the signal-to-noise ratio in real time. If the signal-to-noise ratio is lower than a preset threshold, the rack and pinion mechanism is triggered to perform fine-tuning. Therefore, it can adapt to interference from complex samples. Based on this analyzer, the instrument body uses eight-channel parallel scanning combined with dynamic focusing to significantly shorten the detection time of all indicators for a single sample. It is compatible with various complex biological samples and does not require chemical chromogenic reagents, thus reducing experimental costs and environmental pollution risks. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the analyzer body of the present invention; Figure 3 This is a schematic diagram of the sealing seat of the present invention; Figure 4 This is a schematic diagram of the arc-shaped slide rail of the present invention; Figure 5 This is a schematic diagram of the structure of the double-layer reagent card substrate of the present invention; Figure 6 This is a cross-sectional view of the analyzer body of the present invention; Figure 7 This is the present invention.Figure 6 Enlarged view of point A in the middle; Figure 8 This is a schematic diagram of the rotating ring structure of the present invention; Figure 9 This is the present invention. Figure 8 Enlarged view of point B in the middle.

[0022] In the diagram: 1. Analyzer body; 2. Feed conveyor belt; 4. Sealing seat; 5. Dome-shaped fluorescence collector; 6. Narrow-band filter; 7. Indium gallium arsenide photoelectric sensor; 8. Rotating ring; 9. Arc-shaped slide rail; 10. Light source slider; 11. Piezoelectric focusing lens assembly; 12. Laser rangefinder; 13. Fiber optic beam splitter; 14. Miniature air pump; 15. Double-layer reagent card substrate; 16. Serpentine microfluidic channel; 17. Reflection-enhancing prism; 18. Shape memory alloy; 19. Thermosensitive actuator array; 20. 21. Limiting frame; 22. Lifting assembly; 23. Linear motor; 24. Support frame; 25. First guide rod; 26. First spring; 27. Rack; 28. First gear; 29. ​​Feedback stepper motor; 30. Gear ring; 31. Second gear; 32. Main shaft; 33. Worm gear; 34. Stepper motor; 35. Worm; 36. Connecting shaft; 37. Sleeve; 38. Electric push rod; 39. Adapter rod; 40. Fixed column; 41. Second guide rod; 42. Second spring; 43. Limiting block. Detailed Implementation

[0023] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0024] Example 1: like Figures 1-3As shown, a near-infrared fluorescence immunoassay analyzer includes an analyzer body 1. A sealing seat 4 is mounted on the top of the analyzer body 1. A dome-shaped fluorescence collector 5 is installed inside the cavity of the sealing seat 4. A narrow-band filter 6 is installed at the bottom of the dome-shaped fluorescence collector 5. An arc-shaped slide rail 9 is rotatably connected inside the cavity of the sealing seat 4. A light source slider 10 is mounted on the outer wall of the arc-shaped slide rail 9. A piezoelectric focusing lens group 11 is mounted at the bottom of the light source slider 10. A laser rangefinder 12 and an optical fiber beam splitter 13 are respectively mounted on both sides of the light source slider 10. A feeding conveyor belt 2 is installed inside the cavity of the analyzer body 1. The feeding conveyor belt 2 is located at the top of the adapter rod 38. A support frame 23 is used for the middle... The feed conveyor belt 2 is located at the top of one end of the support frame 23. Indium gallium arsenide photoelectric sensors 7 are symmetrically installed on both sides of the bottom of the dome-shaped fluorescent collector 5. A laser diode is installed at the bottom of the inner cavity of the light source slider 10. The output end of the laser diode is located at the top of the piezoelectric focusing lens group 11. A self-cleaning lens is installed at the bottom of the fiber beam splitter 13. A miniature air pump 14 is installed on the side of the bottom of the self-cleaning lens. The output end of the laser diode is also connected to a single-mode fiber through an FC / APC fiber optic connector to transmit the laser light source emitted by it to the fiber beam splitter 13. The fiber beam splitter 13 is used to split the laser light source emitted by the laser diode into eight independent beams. The fiber optic beam splitter 13 divides a single laser into eight independent beams, which are simultaneously projected onto different areas of the dual-layer reagent card substrate 15 through a self-cleaning lens, achieving coverage of eight times the detection area in a single scan. This significantly shortens the detection time for the entire sample. The narrow-band filter 6 can perform real-time focusing to compensate for deformation or liquid refraction changes in the dual-layer reagent card substrate 15, ensuring that the laser focus is always accurately located at the reaction interface and avoiding signal defocusing loss.

[0025] Example 2: like Figures 1-5As shown, a near-infrared fluorescence immunoassay analyzer has a double-layer reagent card substrate 15 mounted at the center of the top of the analyzer body 1. A serpentine microfluidic channel 16 is formed at the top of the double-layer reagent card substrate 15. A shape memory alloy 18 is mounted at the bottom of the double-layer reagent card substrate 15, and a thermal actuator array 19 is mounted at the bottom of the shape memory alloy 18. Reflection-enhancing prisms 17 are symmetrically mounted on both sides of the inner cavity of the serpentine microfluidic channel 16, positioned at a 45-degree angle within the inner cavity of the serpentine microfluidic channel 16. The reflection-enhancing prisms 17 are used to reflect the lateral scattered light source within the inner cavity of the serpentine microfluidic channel 16 to the dome-shaped fluorescence collector 5. The bottom of the reflection-enhancing prism 17 is attached to the bottom of the inner cavity of the serpentine microfluidic channel 16, and the top of the reflection-enhancing prism 17 is higher than the serpentine microfluidic channel 16. A transparent PDMS layer is installed at the bottom of the inner cavity of the serpentine microfluidic channel 16. The thermal actuator array 19 includes sixty-four units, which are arranged in a grid to cover the bottom of the double-layer reagent card substrate 15. The thermal actuator array 19 contacts the shape memory alloy 18 through the contacts of the units. The double-layer reagent card substrate 15 is a flexible substrate. The temperature control range of the thermal actuator array 19 is 25℃-42℃, which is used to maintain the required temperature for at least 20 minutes to complete the formation of the sandwich immune complex. The laser diode in the light source slider 10 is used to emit 785nm near-infrared laser to excite fluorescent markers. The piezoelectric focusing lens group 11 is used to dynamically adjust the focal length to compensate for the focal plane shift caused by the deformation of the double-layer reagent card substrate 15. The fiber beam splitter 13 is used to divide the single laser into 8 paths. The bottom of the self-cleaning lens is coated with a hydrophobic coating to prevent droplet adhesion. The micro air pump 14 is used to maintain the self-cleaning lens and keep the light transmittance of the self-cleaning lens greater than 95%. The dome-shaped fluorescence collector 5 is used to collect the fluorescence scattered laterally by the reflection enhancement prism 17. Its bottom is a parabolic curved surface used to focus the light spot. The focused light spot is transmitted to the narrow band filter 6 through the outlet. The narrow band filter 6 is used to filter out stray light. The indium gallium arsenide photoelectric sensor 7 is used to convert the fluorescence into an electrical signal. The dual-layer reagent card substrate 15 integrates a serpentine microfluidic channel 16, a shape memory alloy 18, a thermal actuator array 19, and a transparent PDMS layer. The serpentine microfluidic channel 16 is a single-channel continuous serpentine design, with its curved sections inducing Dean vortices. The reflection-enhancing prism 17 is set at a 45-degree angle to improve reflectivity. The thermal actuator array 19 has 64 independently temperature-controlled units to drive the local contraction of the shape memory alloy 18. The thermal actuator array 19 also monitors the temperature changes of each area in real time through independent temperature control units. When a local temperature deviation is detected to exceed a set threshold, the current output is automatically adjusted. When the thermal actuator array 19 detects deformation error, it changes the phase transition state of the shape memory alloy 18 by local heating. The streptavidin-biotin complex on the inner wall of the serpentine microfluidic channel 16 is used to specifically bind to the antigen in the sample. The quantum dot-labeled detection antibody forms a sandwich complex, which is maintained at its optimal reaction temperature by the thermal actuator array 19.

[0026] Example 3: like Figure 1 , Figures 6-9As shown, a near-infrared fluorescence immunoassay analyzer has a rotating ring 8 installed at the bottom of an arc-shaped slide rail 9, a gear ring 29 installed at the bottom of the rotating ring 8, a second gear 30 meshing with the side of the gear ring 29, and a worm gear 32 installed at the bottom of the second gear 30. A connecting shaft 35 is rotatably connected to the inner cavity of the analyzer body 1, and a sleeve 36 is fitted on the outer wall of the connecting shaft 35. A lifting assembly 21 is attached to the bottom of the double-layer reagent card base 15. Limiting frames 20 are symmetrically arranged on both sides of the lifting assembly 21, and a support frame 23 is arranged on the opposite side of the two limiting frames 20. A main shaft 31 is installed in the center of the second gear 30, and a worm gear 32 is installed on the outer wall of the main shaft 31. The bottom of the inner cavity of the analyzer body 1... A stepper motor 33 is installed, which is connected to the main shaft 31 via a coupling. One end of the connecting shaft 35 is rotatably connected to the inner cavity of the analyzer body 1, and the other end of the connecting shaft 35 is equipped with a worm gear 34, which meshes with a worm wheel 32. An electric push rod 37 is installed at the center of the bottom of the sleeve 36. The top of the electric push rod 37 penetrates the outer wall of the sleeve 36 and fits against the outer wall of the connecting shaft 35. Fixed posts 39 are symmetrically installed at both ends of the top of the sleeve 36. A second guide rod 40 is installed in the inner cavity of the fixed post 39, and a second spring 41 is wound around the outer wall of the second guide rod 40. Adaptor rods 38 are symmetrically and movably connected to the bottom of both ends of the lifting assembly 21, and the adapter rods 38 are close to the fixed posts 39. One end of the second spring 41 is movably connected to the inner cavity of the fixed column 39 and sleeved on the outer wall of the second guide rod 40. The top of the second spring 41 is connected to the adapter rod 38, and the bottom of the second spring 41 is installed at the bottom of the inner cavity of the fixed column 39. Limiting blocks 42 are symmetrically installed around the top of the inner cavity of the analyzer body 1. The bottom of the limiting blocks 42 is attached to the top of the double-layer reagent card base 15. The top of the support frame 23 is attached to the bottom of the double-layer reagent card base 15. A linear motor 22 is installed on the side of the limiting frame 20 near the support frame 23. The output end of the linear motor 22 is connected to the support frame 23. The bottom of the inner cavities at both ends of the lifting assembly 21 is symmetrically equipped with first guide rods 24. The outer wall of the first guide rod 24 is... The first spring 25 is wound around the other end of the adapter rod 38, which is movably connected to the inner cavity of the lifting assembly 21 and sleeved on the outer wall of the first guide rod 24. The top of the first spring 25 is connected to the adapter rod 38, and the bottom of the first spring 25 is installed at the bottom of the inner cavity of the lifting assembly 21. The tops of both sides of the lifting assembly 21 are inclined, and the bottoms of the opposite sides of the two limiting frames 20 are also inclined. A feedback stepper motor 28 is installed in the inner cavity of the analyzer body 1. A first gear 27 is installed on the feedback stepper motor 28 through the first rotating shaft. A rack 26 is movably connected in the inner cavity of the analyzer body 1. The rack 26 meshes with the first gear 27. The first gear 27 is located at the bottom of the lifting assembly 21. The lifting assembly 21 is used to lift the center of the double-layer reagent card substrate 15 to produce a parabolic deformation, and the rack 26 is used to assist in compensating for deformation errors.

[0027] Example 4: The samples injected into the serpentine microfluidic channel 16 include novel materials and biological samples. The biological samples include serum, and the novel materials include fluorescently labeled nanomaterials, biocompatible polymers, hydrogels, etc.

[0028] It should be noted that the present invention is a near-infrared fluorescence immunoassay analyzer. In use, the sample is injected into the serpentine microfluidic channel 16, the double-layer reagent card substrate 15 containing the serum sample is placed at the feed conveyor belt 2, the feed conveyor belt 2 is started to continuously transport it to the top of the support frame 23, the linear motor 22 is started, and the double-layer reagent card substrate 15 is driven to rise through the support frame 23, and the double-layer reagent card substrate 15 is limited by the limiting block 42. The thermal actuator array 19 is activated to preheat the double-layer reagent card substrate 15 to 40°C in order to avoid hysteresis of the initial deformation of the shape memory alloy 18. Start the stepper motor 33, which drives the worm gear 32 and the second gear 30 to rotate through the main shaft 31. After the second gear 30 rotates, it meshes with the gear ring 29, so that the gear ring 29 can drive the arc slide rail 9 to rotate through the rotating ring 8. At the same time, start the arc slide rail 9, and move the piezoelectric focusing lens group 11 to the center of the top of the double-layer reagent card substrate 15 through the light source slider 10. When the worm gear 32 rotates, it meshes with the worm 34. Based on this, the sleeve 36 is driven to rotate through the connecting shaft 35. After the sleeve 36 rotates, it drives the lifting assembly 21 to rotate through the fixed column 39 and the adapter rod 38. After the inclined top of the lifting assembly 21 comes into contact with the inclined side of the limiting frame 20, it will be squeezed, so that the lifting assembly 21 can drive one end of the adapter rod 38 to move in the inner cavity of the fixed column 39. When the lifting assembly 21 moves between the two limiting frames 20, the second spring 41 can push the lifting assembly 21 to the top through the adapter rod 38. At this time, the electric push rod 37 is activated to disengage it from the outer wall of the connecting shaft 35. When the connecting shaft 35 rotates, it will not drive the sleeve 36 to rotate, so that the lifting assembly 21 can always be located between the two limiting frames 20. After the upward-splashing lifting component 21 contacts the double-layer reagent card substrate 15, it can lift the middle part of the double-layer reagent card substrate 15 to form a parabolic surface. The piezoelectric focusing lens group 11 adjusts the focus in real time according to the state of the double-layer reagent card substrate 15. At this time, the reflection enhancement prism 17 reflects the fluorescence to the dome-shaped fluorescence collector 5, and the narrow band filter 6 filters out stray light. The indium gallium arsenide photoelectric sensor 7 collects signal-to-noise ratio data in real time. When the signal-to-noise ratio data is less than 10, the feedback stepper motor 28 is activated so that the first gear 27 can mesh with the rack 26. Based on this, the lifting assembly 21 is driven to lift further, and the state of the double-layer reagent card substrate 15 is adjusted again. At the same time, the piezoelectric focusing lens group 11 continues to focus in real time.

[0029] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. A near-infrared fluorescence immunoassay analyzer comprising an analyzer body (1), characterized in that: The middle of the top of the analyzer body (1) is provided with a double-layer reagent card matrix (15), the top of the double-layer reagent card matrix (15) is provided with a serpentine micro-flow channel (16), the bottom of the double-layer reagent card matrix (15) is provided with a shape memory alloy (18), the bottom of the shape memory alloy (18) is provided with a thermal sensitive actuator array (19), and the inner wall of the serpentine micro-flow channel (16) is coated with an antibody; The top of the analyzer body (1) is provided with a sealing seat (4), the inner cavity of the sealing seat (4) is provided with a dome-shaped fluorescence collector (5), the bottom of the dome-shaped fluorescence collector (5) is provided with a narrow-band filter (6), and the inner cavity of the sealing seat (4) is rotatably connected with an arc-shaped sliding rail (9), and the outer wall of the arc-shaped sliding rail (9) is provided with a light source sliding block (10). The bottom of the arc-shaped sliding rail (9) is provided with a rotating ring (8), the bottom of the rotating ring (8) is provided with a gear ring (29), the side edge of the gear ring (29) is engaged with a second gear (30), the bottom of the second gear (30) is provided with a worm gear (32), the inner cavity of the analyzer body (1) is rotatably connected with a connecting shaft (35), the outer wall of the connecting shaft (35) is sleeved with a sleeve (36), the bottom of the double-layer reagent card matrix (15) is attached with a jacking assembly (21), and the two sides of the jacking assembly (21) are symmetrically provided with limiting frames (20), and the opposite sides of the two limiting frames (20) are provided with support frames (23).

2. The near-infrared fluorescent immunoassay apparatus according to claim 1, characterized by: The bottom of the light source sliding block (10) is provided with a piezoelectric focusing lens group (11), the two sides of the light source sliding block (10) are respectively provided with a laser range finder (12) and a fiber optic beam splitter (13), the inner cavity of the analyzer body (1) is provided with a feeding conveyor belt (2), the feeding conveyor belt (2) extends to the top of one end of the support frame (23), the feeding conveyor belt (2) is located at the top of the adaptive rod (38), and the two sides of the bottom of the dome-shaped fluorescence collector (5) are symmetrically provided with indium gallium arsenide photoelectric sensors (7).

3. The near-infrared fluorescent immunoassay apparatus according to claim 2, characterized by: The inner cavity of the light source sliding block (10) is provided with a laser diode, the output end of the laser diode is located at the top of the piezoelectric focusing lens group (11), the bottom of the fiber optic beam splitter (13) is provided with a self-cleaning lens, the side edge of the bottom of the self-cleaning lens is provided with a micro air pump (14), the output end of the laser diode is also connected with a single-mode optical fiber through an FC / APC optical fiber connector, which is used to transmit the laser light source emitted by the laser diode to the fiber optic beam splitter (13), and the fiber optic beam splitter (13) is used to divide the laser light source emitted by the laser diode into eight independent light beams.

4. The near-infrared fluorescent immunoassay apparatus according to claim 1, characterized by: The two sides of the serpentine micro flow channel (16) cavity are symmetrically provided with reflective enhancement prisms (17), which are arranged at an angle of 45 degrees in the inner cavity of the serpentine micro flow channel (16), and are used for reflecting the lateral scattering light source in the serpentine micro flow channel (16) to the dome type fluorescence collector (5). The reflective enhancement prisms (17) are higher than the serpentine micro flow channel (16), and the bottom of the serpentine micro flow channel (16) cavity is provided with a transparent PDMS layer. The antibody in the serpentine micro flow channel (16) is a streptavidin-biotin complex, which is used for binding quantum dot labeled detection antibody.

5. The near-infrared fluorescence immunoassay analyzer according to claim 4, characterized by: The thermal actuator array (19) includes sixty-four units arranged in a grid to cover the bottom of the double-layer reagent card substrate (15), and the thermal actuator array (19) is in contact with the shape memory alloy (18) through the contacts of the units. The double-layer reagent card substrate (15) is a flexible substrate.

6. The near-infrared fluorescent immunoassay instrument according to claim 1, characterized in that: The second gear (30) is centrally provided with a main shaft (31), the outer wall of the main shaft (31) is provided with a worm gear (32), the bottom of the inner cavity of the analyzer body (1) is provided with a stepping motor (33), the stepping motor (33) is connected with the main shaft (31) through a shaft coupling, one end of the connecting shaft (35) is rotatably connected in the inner cavity of the analyzer body (1), the other end of the connecting shaft (35) is provided with a worm (34), and the worm (34) is engaged with the worm gear (32).

7. The near-infrared fluorescent immunoassay apparatus according to claim 6, characterized by: The bottom of the sleeve (36) is centrally provided with an electric push rod (37), the top of the electric push rod (37) penetrates through the outer wall of the sleeve (36) and is attached to the outer wall of the connecting shaft (35), the top of the sleeve (36) is symmetrically provided with a fixed column (39) at both ends, the inner cavity of the fixed column (39) is provided with a second guide rod (40), the outer wall of the second guide rod (40) is wound with a second spring (41), the bottom of both ends of the jacking assembly (21) is symmetrically movably connected with an adaptive rod (38), one end of the adaptive rod (38) close to the fixed column (39) is movably connected in the inner cavity of the fixed column (39) and is sleeved on the outer wall of the second guide rod (40), the top of the second spring (41) is connected with the adaptive rod (38), and the bottom of the second spring (41) is installed at the bottom of the inner cavity of the fixed column (39).

8. The near-infrared fluorescent immunoassay analyzer according to claim 1, characterized by: The top of the inner cavity of the analyzer body (1) is symmetrically provided with a limiting block (42) around, the bottom of the limiting block (42) is attached to the top of the double-layer reagent card substrate (15) around, the top of the support frame (23) is attached to the bottom of the double-layer reagent card substrate (15), and the side of the limiting frame (20) close to the support frame (23) is provided with a linear motor (22), and the output end of the linear motor (22) is connected with the support frame (23).

9. The near-infrared fluorescence immunoassay analyzer according to claim 7, characterized by: The bottom of the inner cavity of both ends of the jacking assembly (21) is symmetrically provided with a first guide rod (24), the outer wall of the first guide rod (24) is wound with a first spring (25), the other end of the adapter rod (38) is movably connected in the inner cavity of the jacking assembly (21) and is sleeved on the outer wall of the first guide rod (24), the top of the first spring (25) is connected with the adapter rod (38), the bottom of the first spring (25) is mounted on the bottom of the inner cavity of the jacking assembly (21), the top of both sides of the jacking assembly (21) is inclined, and the bottom of the opposite side of the two limiting frames (20) is also inclined.

10. The near-infrared fluorescent immunoassay instrument according to claim 1, characterized by: The inner cavity of the analyzer body (1) is provided with a feedback stepping motor (28), the feedback stepping motor (28) is provided with a first gear (27) through a first rotating shaft, the inner cavity of the analyzer body (1) is movably connected with a rack (26), the rack (26) and the first gear (27) are mutually engaged, and the first gear (27) is located at the bottom of the jacking assembly (21).

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