Effect of uracholine on pancreatic secretion

Abstract

The invention relates to the technical field of animal nutrition, and provides an effect of uracholine on pancreas secretion, which is used for promoting animal pancreas secretion and digestion functions. The function of promoting pancreatic secretion and digestion comprises improvement of the activity of pancreatic digestive enzymes, and the pancreatic digestive enzymes comprise one or more of trypsin, amylase and lipase; according to the feed additive, 100 mg / kg to 1000 mg / kg of the feed additive is used in a single metering mode; the animals comprise one of poultry and pigs, the poultry comprise one of broilers, laying hens or ducks, and the broilers are fed with brooding feed for 0-21 days and fattening feed for 22-42 days. According to the technical scheme, the problems that in the prior art, digestive system development of young animals is not perfect, pancreas functions are relatively weak, endogenous digestive enzyme secretion is insufficient, feed nutrient substances are often incompletely digested, diarrhea is caused, and growth is slow are solved.

Description

Technical Field

[0001] This invention relates to the field of animal nutrition technology, specifically to the effect of uracholine on pancreatic secretion. Background Technology

[0002] In intensive animal husbandry, the growth rate of animals and feed conversion efficiency are key factors that determine economic benefits; efficient digestion and absorption of nutrients are the foundation for ensuring good growth performance of animals, and this process largely depends on the integrity and activity of the endogenous enzyme system in the digestive tract; the pancreas, as the most important digestive gland, secretes trypsin, pancreatic amylase, pancreatic lipase, etc., which are the core enzymes for breaking down proteins, carbohydrates and fats. For young animals (such as early-weaned piglets and chicks), their digestive systems are not yet fully developed, their pancreatic function is relatively weak, and their secretion of endogenous digestive enzymes is insufficient, often leading to incomplete digestion of feed nutrients and causing problems such as diarrhea and stunted growth. Similarly, under certain pathological conditions or stress conditions, the pancreatic function of adult animals may also be suppressed. Therefore, finding substances that can safely and effectively stimulate pancreatic exocrine function and increase the activity of endogenous digestive enzymes is of great significance for improving animal health and increasing breeding efficiency.

[0003] Uracholine, chemically known as clobecholine, is a parasympathomimetic drug or cholinergic agonist. Simply put, it's a drug that mimics the action of a neurotransmitter called acetylcholine in our bodies. It directly activates muscarinic acetylcholine receptors in the body, producing an effect similar to parasympathetic nerve excitation. A key chemical property is that it is not easily hydrolyzed by cholinesterase. Our bodies have an enzyme (acetylcholinesterase) that rapidly breaks down natural acetylcholine, thus terminating its effect. However, uracholine resists this breakdown, making its effects more prolonged and stable. Its core function is to stimulate pancreatic juice secretion. The exocrine part of the pancreas is responsible for producing and secreting pancreatic juice rich in digestive enzymes and bicarbonate, which is crucial for food digestion (especially of fats and proteins). Uracholine directly affects this process in the following ways: 1) Stimulates proenzyme secretion: This is its primary function. Pancreatic acinar cells are the factories that synthesize and store digestive enzymes (such as trypsinogen, pancreatic lipase, and pancreatic amylase). These cell membranes are rich in M3 muscarinic receptors. When clobetacholine binds to these receptors, it activates intracellular signaling pathways, leading to an increase in intracellular calcium ion concentration. Calcium ions, acting as key messengers, trigger the fusion of zymogen granules with the cell membrane, thereby releasing large amounts of digestive enzymes into the pancreatic duct system. Simply put, it "commands" the pancreas to release digestive enzymes.

[0004] 2) Potential effects on water and electrolyte secretion: The central cells of the pancreatic duct are responsible for secreting a bicarbonate-rich fluid to neutralize the acidic chyme from the stomach. This process is more complex to regulate, primarily controlled by the hormone secretin. Acetylcholine (and uracholine) have relatively weaker effects on this part, but they can indirectly enhance bicarbonate secretion through mechanisms such as increased blood flow or enhanced cellular sensitivity to secretin. Currently, the application of uracholine is mainly focused on clinical settings in humans.

[0005] Surprisingly, to date, no studies have reported or suggested that uracholine promotes the exocrine function of the pancreas in animals.

[0006] The digestive system of young animals is not yet fully developed, and their pancreatic function is relatively weak. They also have insufficient secretion of endogenous digestive enzymes, which often leads to incomplete digestion of feed nutrients, causing diarrhea and stunted growth. Therefore, we proposed the effect of uracholine on pancreatic secretion. Summary of the Invention

[0007] This invention proposes the effect of uracholine on pancreatic secretion, which solves the problem mentioned in the background art that the digestive system of existing young animals is not fully developed, the pancreatic function is relatively weak, and the secretion of endogenous digestive enzymes is insufficient, which often leads to incomplete digestion of feed nutrients, causing diarrhea and stunted growth.

[0008] The technical solution of the present invention is as follows: Uracholine's effect on pancreatic secretion: Uracholine is added to feed to promote pancreatic secretion and digestive function in animals.

[0009] As a further technical solution of the present invention, the promotion of pancreatic secretion and digestive function includes increasing the activity of pancreatic digestive enzymes, wherein the pancreatic digestive enzymes include one or more of trypsin, amylase and lipase.

[0010] As a further technical solution of the present invention, the feed additive is used in a single dose at 100 mg / kg-1000 mg / kg of feed.

[0011] As a further technical solution of the present invention, the animal includes one of poultry and pig, and the poultry includes one of broiler chickens, laying hens or ducks.

[0012] As a further technical solution of the present invention, the feed composition is used to feed young animals.

[0013] As a further technical solution of the present invention, the feeding of broilers is divided into brooding feed for 0-21 days and fattening feed for 22-42 days.

[0014] As a further technical solution of the present invention, the formula of the brooder feed is: 55%-57% corn, 30%-35% soybean meal, 1.4%-1.5% dicalcium phosphate, 1%-1.1% limestone powder, 2%-3% vegetable oil, 0.3% salt, and 1% premix.

[0015] As a further technical solution of the present invention, the fertilizer formula is as follows: corn 60%-62%, soybean meal 25%-28%, wheat bran 3%-5%, dicalcium phosphate 1.3%, limestone powder 1.2%, vegetable oil 3%-4%, salt 0.3%, and premix 1%.

[0016] As a further technical solution of the present invention, each kilogram of the premix contains 1,500,000 IU of vitamin A, 400,000 IU of vitamin D3, 3,300 IU of vitamin E, 3,300 mg of vitamin K3, 9.5 g of iron, 6.5 g of copper, 9 g of zinc, 12 g of manganese, 33 mg of selenium, 150 mg of iodine, 70% of lysine, 350 g of lysine hydrochloride, 134 g of methionine, 70 g of threonine, and 30 g of tryptophan.

[0017] As a further technical solution of the present invention, the broiler chickens are fed with water at a rate of not less than 1.6 times the amount of feed. The 21st to 23rd day of feeding is a transition period. The feed during the transition period is brooder feed VIII on the 21st day, brooder feed VIII and fattening feed VIII on the 22nd day, and then transitions to feeding only fattening feed on the 23rd day.

[0018] The working principle and beneficial effects of this invention are as follows: 1. In this invention, uracholine is added as an additive to broiler feed. The broiler chickens are divided into two age groups: 0-22 days and 23-42 days. They are fed brooder feed and fattening feed respectively. By adding a certain amount of uracholine to their feed, the pancreatic secretion and digestive function of the broilers can be effectively promoted, resulting in better growth of the broilers.

[0019] 2. In this invention, uracholine is added as an additive to broiler feed. When white-feathered broilers are tested, the levels of trypsin, lipase, and amylase in their duodenum, jejunum, and pancreas all show an increase compared to broilers without the additive. After 42 days of feeding, the production performance of the broilers is significantly improved. Attached Figure Description

[0020] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0021] Figure 1 This is a camera image showing the feeding of broiler chickens according to the present invention; Figure 2 For the present invention Figure 1 A camera view from another angle; Figure 3 This is an anatomical diagram of the duodenum and pancreas of a broiler chicken according to the present invention. Figure 4 This is a schematic diagram showing the appearance and inner wall of the digestive tract (a) and cecum (b) of the white-feathered broiler chicken of the present invention.

[0022] Figure 4 In the middle: 1. Esophagus; 2. Croup; 3. Proventricle; 4. Gizzard; 5. Meckel's process (remnant of the yolk sac); 6. Small intestine; 7. Cecum; 8. Rectum. B: The arrow indicates the spiral crest, a protrusion on the inner wall of the cecum. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0024] It should be noted that in the following embodiments and comparative examples, any aspects not mentioned, such as feeding climate and temperature, must be consistent with the environment and climate suitable for poultry farming, as well as the feeding-related equipment used, to ensure the rigor of the experiment. Furthermore, in the following examples, all feed ingredients involved in this invention were purchased from the same batch and the same manufacturer.

[0025] Those skilled in the art should understand that these embodiments are merely illustrative of the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. The scope of protection of the present invention is defined by the claims.

[0026] (a) Identifying the problem The inventors of this application have discovered that the addition of uracholine to broiler feed can significantly improve the production performance of broilers and effectively enhance the secretion of digestive enzymes and pancreatic function. Currently, there is no reliable method to increase the secretion of digestive enzymes and improve pancreatic function in broilers.

[0027] Based on the above, this application uses uracholine as an additive in the feed of broiler chickens to investigate its effects on the secretion of digestive enzymes and pancreatic function in broilers.

[0028] In this application, uracholine is added to the feed as an additive to enhance the secretion of digestive enzymes in broilers and improve their pancreatic function.

[0029] To verify the effects of the above embodiments, the test materials and reagents used in the following embodiments, unless otherwise specified, can be obtained commercially. Any techniques or conditions not specifically mentioned in the embodiments can be performed according to the techniques or conditions described in the literature in this field or according to the product instructions.

[0030] (II) Experimental Objective: (1) To determine the effect of different dosages of uracholine on promoting the secretion of digestive enzymes by the pancreas in broilers; (2) To investigate the optimal amount of uracholine added to broilers under the basal diet of corn-soybean meal; (3) Explore and reveal the mechanism of action of uracholine on the production performance and pancreatic function of broilers.

[0031] (III) Experimental Design: The experiment selected 420 one-day-old AA broilers from the same batch and randomly divided them into 5 treatment groups: a control group (corn-soybean meal basal diet), and control groups supplemented with 100g / kg, 200g / kg, and 300g / kg of uracholine, respectively. Each group had 6 replicates, with 14 chickens in each replicate. The experiment was conducted in two stages: 0d-21d and 22d-42d.

[0032] (iv) Measurement indicators: This experiment mainly determined the effects and mechanisms of action of different amounts of uracholine additives on broiler production performance, promotion of trypsin secretion, promotion of pancreatic cell synthesis, improvement of digestive capacity and pancreatic function under basal diet feeding conditions.

[0033] (V) Test Results: In this study, SPSS 22.0 software was used to perform statistical analysis on the data of each group. One-way ANOVA was used for statistical analysis, and linear and quadratic terms were compared. Ducan's multiple comparison analysis was used to analyze the differences between treatments.

[0034] That is, when P < 0.05, the difference is considered significant, and P values ​​between 0.05 and 0.1 are classified as trends.

[0035] The relevant data obtained after the experiment are as follows: Table 1. Effects of different doses of uracholine added to the diet on broiler production performance.

[0036] The table above shows that under the feeding conditions of a corn-soybean meal basal diet, the addition of an appropriate dose of uracholine can significantly improve the production performance of broilers. Its mechanism of action is closely related to promoting digestive enzyme secretion and optimizing pancreatic function. Table 1 data shows that during the entire trial period of 0–42 days, the broiler group treated with 200 g / kg uracholine showed the best performance in terms of body weight (3.117 kg) and stage weight gain, and the feed conversion ratio (1.345) was significantly lower than other groups (P < 0.001), indicating that this level of addition can effectively improve feed conversion efficiency and promote growth.

[0037] From a physiological perspective, uracholine, as a cholinergic receptor agonist, can mimic the effects of acetylcholine, stimulating the contraction of smooth muscle and glandular secretion in the digestive tract, thereby enhancing gastrointestinal motility and promoting the secretion of digestive juices such as pancreatic juice and bile.

[0038] Pancreatic juice contains abundant proteases, amylases, and lipases. Increased secretion of these enzymes helps to more fully break down nutrients in the diet, improving the digestibility and absorption of protein, starch, and fat. This not only directly results in weight gain and a lower feed conversion ratio but also reduces the metabolic burden on the pancreas, indirectly optimizing pancreatic function.

[0039] Table 2. Effects of dietary urethanecholine supplementation on digestive enzymes in broilers.

[0040] In summary, the above results indicate that under the feeding conditions of a corn-soybean meal basal diet, the optimal supplementation dose of uracholine is 200 g / kg; the addition of 200 g / kg of uracholine to the diet can significantly improve the production performance of broilers and effectively enhance the secretion of digestive enzymes and improve pancreatic function.

[0041] It should be noted that in this application, adding a specific dose of uracholine to animal diets can significantly stimulate pancreatic tissue and increase the activity of a variety of key digestive enzymes, thereby providing a novel technical solution for solving the aforementioned industrial problems such as feeding young to adult animals.

[0042] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. The effect of uracholine on pancreatic secretion, characterized in that, Uracholine is added to feed to promote pancreatic secretion and digestive function in animals.

2. The effect of uracholine on pancreatic secretion according to claim 1, characterized in that, The promotion of pancreatic secretion and digestive function includes increasing the activity of pancreatic digestive enzymes, including one or more of trypsin, amylase, and lipase.

3. The effect of uracholine on pancreatic secretion according to claim 1, characterized in that, The feed additive is used in single doses of 100 mg / kg to 1000 mg / kg of feed.

4. The effect of uracholine on pancreatic secretion according to claim 3, characterized in that, The animals mentioned include one of poultry and pigs, and the poultry includes one of broiler chickens, laying hens, or ducks.

5. The effect of uracholine on pancreatic secretion according to claim 4, characterized in that, The feed composition is used to feed young animals.

6. The effect of uracholine on pancreatic secretion according to claim 4, characterized in that, Broiler feeding is divided into brooder feed for 0-21 days and fattening feed for 22-42 days.

7. The effect of uracholine on pancreatic secretion according to claim 6, characterized in that, The formula for the brooder feed is as follows: 55%-57% corn, 30%-35% soybean meal, 1.4%-1.5% dicalcium phosphate, 1%-1.1% limestone powder, 2%-3% vegetable oil, 0.3% salt, and 1% premix.

8. The effect of uracholine on pancreatic secretion according to claim 6, characterized in that, The fertilizer formula is as follows: corn 60%-62%, soybean meal 25%-28%, wheat bran 3%-5%, dicalcium phosphate 1.3%, limestone powder 1.2%, vegetable oil 3%-4%, salt 0.3%, and premix 1%.

9. The effect of uracholine on pancreatic secretion according to claim 7 or 8, characterized in that, The premix contains, per kilogram, 1,500,000 IU of vitamin A, 400,000 IU of vitamin D3, 3,300 IU of vitamin E, 3,300 mg of vitamin K3, 9.5 g of iron, 6.5 g of copper, 9 g of zinc, 12 g of manganese, 33 mg of selenium, 150 mg of iodine, 70% of lysine, 350 g of lysine hydrochloride, 134 g of methionine, 70 g of threonine, and 30 g of tryptophan.

10. The effect of uracholine on pancreatic secretion according to claim 7 or 8, characterized in that, Provide at least 1.6 times the amount of water when feeding broilers. Days 21 to 23 are the transition period. The feed during the transition period is brooder feed VIII on day 21, brooder feed VIII and fattening feed VIII on day 22, and then transition to feeding only fattening feed on day 23.

Images