Preparation and application of antibacterial film containing plant essential oil characteristic components

Abstract

The invention provides preparation and application of an antibacterial film containing plant essential oil characteristic components, and belongs to the technical field of food packaging materials. Sodium alginate and pectin are used as double-network base materials, glycerol is used as an emulsifier to prepare a film forming solution, and plant essential oil characteristic components obtained after molecular distillation are added into the film forming solution to prepare the slow-release film material containing the plant essential oil. According to the invention, the plant essential oil characteristic component is used as the antibacterial agent, the essential oil characteristic component can be used for realizing refined utilization of plant-derived bioactive components, the potential influence on food caused by introduction of unnecessary components is reduced, and the essential oil antibacterial agent has important significance on inhibition of microbial growth and prolonging of shelf life of products. The film material is a biomass material and still has good film-forming property and mechanical property under the condition that a cross-linking agent is not added, and the prepared slow-release film not only can weaken pungent smell of essential oil, but also can realize slow release of antibacterial components.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of food packaging materials, and particularly relates to a bacteriostatic film containing plant essential oil characteristic components and a preparation method and application thereof. BACKGROUND

[0002] Under the dual driving of consumption upgrading and health awareness awakening, the public's attention to food safety has extended from food itself to the packaging link, and the safety of food additives and packaging materials has become an important indicator for measuring food quality. On the one hand, with the continuous enhancement of people's food safety awareness, synthetic additives such as food preservatives are increasingly rejected by the public due to potential health risks, and the market demand for "additive-free" and "natural preservative" foods continues to rise. On the other hand, current mainstream plastic product packaging materials are not only difficult to degrade in the natural environment, easily causing "white pollution", but also generally lack long-term preservation and antibacterial ability, and cannot meet the quality assurance needs of food during storage and transportation. Under this background, finding and systematically studying green and safe, stable and efficient natural antioxidant and antibacterial packaging materials not only can solve the dual problems of food preservation and environmental protection, but also can meet the pursuit of consumers for healthy life, and has very important practical significance and broad market prospects. Plant essential oils, as a kind of natural volatile aromatic compounds extracted from plant organs, have become a research hotspot in the field of food packaging due to their natural, green and non-toxic side effects, and have irreplaceable research value. Plant essential oils are complex and rich in composition, usually containing terpenes, phenols, aldehydes and other active substances. These components endow them with excellent biological activities such as anti-inflammatory, antibacterial and antioxidant, and therefore they have been widely used in food preservation (inhibition of microbial growth), biological medicine (anti-inflammatory and antibacterial) and cosmetics (natural preservative) industries. However, liquid plant essential oils have obvious performance defects, which greatly limit their direct application in the field of food packaging. Firstly, they have poor stability and are easily affected by external environmental factors such as temperature, light and humidity, leading to oxidation and decomposition and resulting in reduced or lost activity. Secondly, they have strong volatility, which not only causes rapid loss of active ingredients, but also causes their own obvious odor (such as the pungent smell of cinnamon essential oil and the irritating smell of lemon essential oil) to penetrate into food, changing the original flavor of food, and therefore they are not suitable for direct addition to food or simple dispersion on the surface of packaging materials.

[0003] In the actual preparation process of food packaging film material, there are still many problems: on the one hand, due to the improper selection of film-forming matrix material (such as the permeability of the material itself is too strong, and the compatibility with essential oil is poor), the film lacks ideal slow-release ability, resulting in that the antibacterial ingredients either release too fast, the concentration is too high in a short period of time, affecting food safety, or release is blocked, and the antibacterial and fresh-keeping effect cannot be played; on the other hand, the conventional film packaging materials on the market are mainly artificial synthetic high molecular materials such as polyethylene and polypropylene, and a small amount of volatile organic compounds may be released during the processing or use of these materials, producing odors, which can penetrate into the food through the contact between the packaging and the food, affecting the flavor and quality of the food, and being contrary to the concept of "green and safe" packaging. Therefore, it is very important to further improve the comprehensive performance of the packaging material and promote the industrial application of green and safe food packaging materials. SUMMARY

[0004] The object of the present application is to provide a slow-release film material containing plant essential oil, a preparation method and application. The prepared slow-release film has good film-forming property and mechanical property, can reduce the irritating odor of essential oil, and can realize slow release of antibacterial ingredients.

[0005] In order to achieve the above-mentioned object of the application, the present application provides the following technical solutions: The present application provides a slow-release film material containing plant essential oil, and the preparation raw materials of the slow-release film material containing plant essential oil include the following components in mass / volume ratio: The ratio of sodium alginate, pectin, emulsifier, plant essential oil is 1 g:1 g:0.04-1 mL:0.25-10 mL.

[0006] Preferably, the emulsifier includes one of glycerol, glycerol monostearate, sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, Tween-80, polyoxyethylene ether, polyoxypropylene ether, ethylene oxide and propylene oxide block copolymer, polyol fatty acid ester, polyethylene glycol, starch, polyoxyethylene polyoxypropylene ether triblock copolymer.

[0007] Preferably, the plant essential oil is cypress essential oil.

[0008] The present application also provides a preparation method of the above-mentioned slow-release film material containing plant essential oil, characterized in that it comprises the following steps: (1) Dissolve sodium alginate, pectin and emulsifier in water, heat and stir to obtain a film-forming liquid; (2) Add cypress essential oil to the film-forming liquid, stir and ultrasonic to obtain an emulsion; (3) Place the emulsion for curing to obtain a slow-release film material containing plant essential oil.

[0009] Preferably, the volume ratio of the emulsifier to water in step (1) is 0.2 to 1:100.

[0010] Preferably, the heating and stirring temperature in step (1) is 60~80 ℃ and the time is 6~7 h.

[0011] Preferably, the stirring temperature in step (2) is 25~55 ℃ and the time is 20~40 min; the ultrasonic temperature is 25~40 ℃ and the time is 1~3 h.

[0012] Preferably, the temperature for static curing in step (3) is 20~45 ℃ and the time is 24~48 h.

[0013] The present invention also provides the application of the above-mentioned sustained-release film material containing plant essential oil or the sustained-release film material containing plant essential oil prepared by the above preparation method in the preparation of preservation and / or antibacterial products.

[0014] The beneficial effects of this invention compared to the prior art are as follows: (1) This invention uses sodium alginate and pectin as a dual-network substrate and glycerol as an emulsifier to prepare a film-forming solution. Rhododendron essential oil is added to the film-forming solution to prepare a slow-release film material containing plant essential oils. This invention uses characteristic components of plant essential oils as antibacterial agents. Using these characteristic components allows for the refined utilization of plant-derived bioactive components, reducing the potential impact of introducing unnecessary components on food. This is of great significance for inhibiting microbial growth and extending product shelf life.

[0015] (2) The film material of the present invention is made of biomass material, which still has good film-forming properties and mechanical properties without the addition of crosslinking agent. The prepared slow-release film can not only reduce the irritating smell of essential oil, but also achieve the slow release of antibacterial components.

[0016] (3) The preparation process of this invention is simple to operate and the parameters are easy to control, which can meet the needs of mass production, fit the development trend of green packaging, and has extremely high practical application value and broad industrialization prospects in the field of food packaging. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1The present invention provides the preparation process and images of the six types of eucalyptus essential oils in Example 1, wherein a is the preparation process and b is an image of the six types of eucalyptus essential oils. Figure 2 The diagram shows the antibacterial effects of six kinds of eucalyptus essential oils in Experiment Example 1 of this invention, numbered 1 to 6 from left to right. Figure 3 Images of the blank membrane and the sustained-release membrane prepared in Experimental Example 1 of the present invention, where a is the blank membrane and b is the sustained-release membrane; Figure 4 The surface smoothness of the sustained-release membranes at different concentrations in Experimental Example 1 of this invention is shown, where a is 0 μL / mL; b is 10 μL / mL; c is 30 μL / mL; and d is 50 μL / mL. Figure 5 The contact angles between the sustained-release membranes and water at different essential oil characteristic component contents in Experimental Example 1 of this invention are shown below; where a is 0 μL / mL; b is 10 μL / mL; c is 30 μL / mL; and d is 50 μL / mL. Figure 6 This is a schematic diagram illustrating the antibacterial effect of sustained-release films with different essential oil characteristic component contents in Experiment Example 1 of the present invention; Figure 7 This is a schematic diagram illustrating the sustained-release effect of sustained-release membranes with different contents of essential oil characteristic components in Experimental Example 1 of the present invention; Figure 8 This is a schematic diagram of the sustained-release effect of the 50 μL / mL sustained-release membrane in Experimental Example 1 of the present invention; Figure 9 This is a schematic diagram of the water vapor transmission rate of the 50 μL / mL sustained-release membrane in Experiment Example 1 of the present invention; Figure 10 This is a diagram showing the effect of a 50 μL / mL sustained-release membrane on the preservation of green grapes in Experiment Example 1 of this invention. Detailed Implementation

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0021] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0022] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0023] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0024] This invention provides a method for preparing a sustained-release thin-film material containing eucalyptus oil, characterized by comprising the following steps: (1) Preparation of eucalyptus essential oil: (1.1) After enzymatic hydrolysis of the *Rhododendron simsii* powder, the essential oil of *Rhododendron simsii* was extracted using a steam distillation apparatus. The content of the extracted essential oil was determined and the extraction rate was calculated. The enzyme used for enzymatic hydrolysis was one of cellulase, hemicellulase, pectinase, α-amylase, or β-glucosidase. (1.2) Using molecular distillation, the essential oil of *Gynostemma pentaphyllum* from step (1.1) was taken according to... Figure 1 The separation process is shown in the figure. First, the heavy phase component and light phase component 6 of the essential oil of *Gynostemma pentaphyllum* are collected by distillation under the conditions of distillation temperature of 50~70℃, feed rate of 0.5~2 mL / min, rotor speed of 200~300r / min and vacuum degree of 300~400Pa. (1.3) The heavy phase to be separated in (1.2) is subjected to molecular distillation again under the same conditions as in (1.2) to obtain the heavy phase and light phase component 5 to be separated; (1.4) Repeat step (1.2) again to obtain component 4, which consists of the heavy phase and the light phase to be separated; (1.5) Take the heavy phase to be separated from (1.4), keep the feed rate and rotor speed constant, and separate the heavy phase component 1 and the light phase to be separated at 500~700 Pa and 70~90℃; (1.6) Take (1.5) the light phase to be separated, keep the feed rate and rotor speed constant, and separate the light phase component 3 and heavy phase component 2 at 400~600 Pa and 80~95℃.

[0025] (2) Dissolve sodium alginate, pectin and emulsifier in water, heat and stir to obtain film-forming solution; (3) Add eucalyptus oil to the film-forming solution, stir and sonicate to obtain an emulsion; (4) The emulsion was left to stand and cure to obtain a slow-release film material containing plant essential oil.

[0026] Example 1 Example 1 of this invention provides a method for preparing a sustained-release thin film material containing eucalyptus oil, the specific steps of which are as follows: (1) Preparation of Rhododendron essential oil according to Figure 1 The process for separating *Rhododendron simsii* essential oil involved enzymatic hydrolysis of *Rhododendron simsii* powder using pectinase. 50.0 g of *Rhododendron simsii* branch powder was accurately weighed. The enzyme concentration was 1.5%, the pH was 5.5, the material-to-liquid ratio was 1:20, the hydrolysis time was 4 h, and the hydrolysis temperature was 55 ℃. Under these conditions, the extraction rate of *Rhododendron simsii* essential oil reached 1.65%.

[0027] Molecular distillation was used to fractionate the essential oil of *Aglaonema*. The process conditions were: distillation temperature 60 ℃, feed rate 1 mL / min, rotor speed 250 r / min, and vacuum degree 380 Pa. The heavy phase component to be separated and the light phase component 6 were obtained. The above steps were repeated twice under the same conditions to finally obtain the light phase components 4 and 5, as well as the heavy phase to be separated after the third distillation.

[0028] The heavy phase to be separated after the third distillation is taken. The feed rate and rotor speed are kept constant. The vacuum and distillation temperature are then increased. A fourth distillation at 600 Pa and 80 °C yields heavy phase component 1 and a light phase component. The light phase component is then distilled a fifth time at 500 Pa and 90 °C to obtain light phase component 3 and heavy phase component 2. Figure 1 As shown.

[0029] Figure 1 The results show that components 1 and 2 are darker in color and are heavy phase components, while components 3 / 4 / 5 / 6 are light phase components. Component 5 was used in the following experiments.

[0030] (2) Preparation of sustained-release film material containing eucalyptus oil 1.0 g sodium alginate, 1.0 g pectin, and 0.9 mL glycerol were added to 100 mL distilled water and heated and stirred at 60 °C for 6 h to obtain a film-forming solution. 20 mL of the film-forming solution was taken, and the fifth component of the eucalyptus essential oil was added to prepare a solution with an essential oil concentration of 10 μL / mL. The solution was stirred at 25 °C for 30 min, and then sonicated at 500 W for 2 h at 25 °C to obtain a white emulsion. All the white emulsion was added to a petri dish, and the petri dish was incubated in a constant temperature and humidity chamber at 25 °C and 40% humidity for 48 h to obtain a sustained-release film material containing eucalyptus essential oil.

[0031] Example 2 Example 2 of the present invention provides a method for preparing a sustained-release film material containing eucalyptus oil based on the fifth component of the eucalyptus oil described in Example 1. The specific steps are as follows: 1.0 g sodium alginate, 1.0 g pectin, and 0.9 mL Tween-80 were added to 100 mL distilled water and heated and stirred at 60 ℃ for 6 h to obtain a film-forming solution. 20 mL of the film-forming solution was taken, and an appropriate amount of the fifth component of *Rhododendron simsii* essential oil was added to prepare a solution with an essential oil concentration of 10 μL / mL. The solution was stirred at 30 ℃ for 30 min, and then sonicated at 25 ℃ with 200 W power for 2 h to obtain a white emulsion. All the white emulsion was added to a petri dish, and the petri dish was placed in a constant temperature and humidity chamber at 25 ℃ and 40% humidity for 48 h to obtain a sustained-release film material containing *Rhododendron simsii* essential oil.

[0032] Example 3 Example 3 of the present invention provides a method for preparing a sustained-release film material containing eucalyptus essential oil based on the fifth component of the eucalyptus essential oil described in Example 1. The specific steps are as follows: 1.0 g sodium alginate, 1.0 g pectin, and 0.6 mL polyethylene glycol-600 were added to 100 mL distilled water and heated and stirred at 60 °C for 6 h to obtain a film-forming solution. 20 mL of the film-forming solution was taken, and an appropriate amount of the fifth component of *Rhododendron simsii* essential oil was added to prepare a solution with an essential oil concentration of 30 μL / mL. The solution was stirred at 40 °C for 30 min, and then sonicated at 30 °C with 500 W power for 2 h to obtain a white emulsion. All the white emulsion was added to a petri dish, and the petri dish was placed in a constant temperature and humidity chamber at 40 °C and 40% humidity for 48 h to obtain a sustained-release film material containing *Rhododendron simsii* essential oil.

[0033] Example 4 Example 4 of the present invention provides a method for preparing a sustained-release film material containing eucalyptus oil based on the fifth component of the eucalyptus oil described in Example 1. The specific steps are as follows: 1.0 g sodium alginate, 1.0 g pectin, and 0.6 g polyoxyethylene polyoxypropylene ether triblock copolymer were added to 100 mL of distilled water and heated and stirred at 60 °C for 6 h to obtain a film-forming solution. 20 mL of the film-forming solution was taken, and an appropriate amount of the fifth component of *Rhododendron simsii* essential oil was added to prepare a solution with an essential oil concentration of 50 μL / mL. The solution was stirred at 55 °C for 30 min, and then sonicated at 30 °C with 200 W power for 2 h to obtain a white emulsion. All the white emulsion was added to a petri dish, and the petri dish was placed in a constant temperature and humidity chamber at 40 °C and 40% humidity for 48 h to obtain a sustained-release film material containing *Rhododendron simsii* essential oil.

[0034] Example 5 Example 5 of the present invention provides a method for preparing a sustained-release film material containing eucalyptus essential oil based on the fifth component of the eucalyptus essential oil described in Example 1. The specific steps are as follows: 1.0 g sodium alginate, 1.0 g pectin, and 0.1 g glyceryl monostearate were added to 100 mL of distilled water and heated and stirred at 80 °C for 6 h to obtain a film-forming solution. 20 mL of the film-forming solution was taken, and an appropriate amount of the fifth component of *Rhododendron molle* essential oil was added to prepare a solution with an essential oil concentration of 50 μL / mL. The solution was stirred at 55 °C for 30 min, and then sonicated at 40 °C with 500 W power for 2 h to obtain a white emulsion. All the white emulsion was added to a petri dish, and the petri dish was placed in a constant temperature and humidity chamber at 40 °C and 40% humidity for 24 h to obtain a sustained-release film material containing *Rhododendron molle* essential oil.

[0035] Experimental Example 1 Test Example 1 of this invention tested the effect of the sustained-release film containing characteristic components of eucalyptus essential oil prepared in Example 1. The specific steps are as follows: (1) Screening of antibacterial activity of different characteristic components of eucalyptus essential oil Six characteristic components of the essential oil from Example 1 were taken and added to a 0.4% (w / v) Tween-80 solution to prepare 400 μL / mL and 800 μL / mL solutions, respectively. 10 μL of each solution was added dropwise to filter paper. The dried filter paper was then placed on a solid culture medium, which was incubated at 37 ℃ for 24 h. The results are as follows: Figure 2 As shown.

[0036] Figure 2 The results showed that characteristic component 5, as the light phase component, exhibited the best antibacterial effect, with inhibition zone diameters reaching 15 mm and 22 mm at 400 μL / mL and 800 μL / mL, respectively.

[0037] (2) Preparation of sustained-release membranes and blank membranes with different contents of essential oil characteristic components Preparation of sustained-release membranes with different essential oil characteristic component contents: 1.0 g sodium alginate, 1.0 g pectin, and 0.9 mL glycerol were added to 100 mL distilled water and heated and stirred at 60 ℃ for 6 h to obtain a film-forming solution. 20 mL of the film-forming solution was taken, and an appropriate amount of the fifth component of *Rhododendron simsii* essential oil was added to prepare solutions with essential oil concentrations of 10 / 30 / 50 / 100 μL / mL, respectively. After stirring for 30 min, the solutions were sonicated at 60 ℃ for 2 h to obtain a white emulsion. The white emulsion was added to a petri dish, and the petri dish was placed in a constant temperature and humidity chamber at 25 ℃ and 40% humidity for 48 h to obtain a sustained-release film material (sustaining membrane) containing *Rhododendron simsii* essential oil.

[0038] Preparation of blank membrane: 1.0 g sodium alginate, 1.0 g pectin, and 0.9 mL glycerol were added to 100 mL distilled water and heated and stirred at 60 ℃ for 6 h to obtain the film-forming solution. 20 mL of the film-forming solution was added to a petri dish, and the dish was placed in a constant temperature and humidity chamber at 25 ℃ and 40% humidity for 48 h. The results are as follows. Figure 3 As shown.

[0039] Figure 3 The results showed that the slow-release membrane had greater opacity, which was related to the content of essential oils.

[0040] (3) Surface morphology characterization of sustained-release membranes and blank membranes with different contents of essential oil characteristic components A 0.5 × 0.5 cm thin film was cut, sputter-coated with gold, and then subjected to scanning electron microscopy. The results are as follows: Figure 4 As shown.

[0041] Figure 4 The results showed that the surface smoothness of the slow-release film initially increased and then decreased with the increase of essential oil content.

[0042] (4) Hydrophilicity of sustained-release membranes and blank membranes with different contents of essential oil characteristic components Cut a 1.0 × 1.0 cm film and test its hydrophilicity using a contact angle meter. The results are as follows. Figure 5 As shown.

[0043] Figure 5 The results showed that the surface contact angle between the slow-release membrane and water increased with the increase of essential oil content. Compared with the blank membrane, the hydrophilicity of the slow-release membrane decreased slightly, with a maximum contact angle of 26.2°.

[0044] (5) Antibacterial effect of sustained-release membranes with different contents of essential oil characteristic components Prepare a solid culture medium. Add 200 μL of diluted bacterial suspension to the surface of the solid culture medium and spread the suspension evenly using a spreading stick. Cut a 1 cm diameter film and place it in the culture medium. Incubate the culture medium at 37 °C for 24 h.

[0045] Figure 6 The results showed that when the concentration of essential oil characteristic components reached 50 μL / mL, a distinct antibacterial zone appeared near the film, demonstrating effective antibacterial ability.

[0046] (6) Infrared structural characterization of sustained-release membranes and blank membranes with different contents of essential oil characteristic components First, a potassium bromide pellet was used to remove the background. Then, a 2×6 cm film was cut and pressed onto the potassium bromide pellet. A clamp was used to fix it in place, and infrared testing was performed. The results are as follows: Figure 7 As shown.

[0047] Figure 7 The results show that the absorption peak intensity of the oxygen-containing functional groups and double bond structure of the sustained-release membrane increases with the increase of essential oil characteristic components.

[0048] (7) The sustained-release effect of the sustained-release membrane A 50 mg sample of *Aristolochia debilis* film with a concentration of 50 μL / mL of essential oil characteristic components was immersed in 5 mL of ethanol solution. Samples of the solution were taken at 2 h, 4 h, 6 h, 8 h, 10 h, and 12 h for UV absorption rate testing.

[0049] Figure 8 The results showed that, with the increase of time, the release rate of the characteristic components of the essential oil at a concentration of 50 μL / mL was rapidly and gradually reduced after 4 h, demonstrating a long-term sustained-release capability.

[0050] (8) Water vapor transmission rate of the slow-release membrane and the blank membrane Take an appropriate amount of distilled water and place it in centrifuge tubes. Wrap the blank membrane and the sustained-release membrane with an essential oil characteristic component concentration of 50 μL / mL in the centrifuge tubes and weigh them. The control group is not wrapped. After standing for 8 hours, weigh them again and calculate the water loss. The results are as follows: Figure 9 As shown.

[0051] Figure 9 The results showed that the water permeability of the slow-release membrane with an essential oil characteristic component concentration of 50 μL / mL was slightly lower than that of the blank membrane, which is consistent with the microstructure of test 4) and the hydrophilicity of test 5).

[0052] (9) The effect of different packaging films on preserving green grapes Equal masses of green grapes were placed under commercial plastic wrap, blank film, and a slow-release film with a concentration of 50 μL / mL essential oil characteristic components, and stored at room temperature. The grapes were observed daily for bacterial growth and freshness. If the green grapes showed signs of spoilage, the time and a photograph were recorded. Results are as follows: Figure 10 As shown.

[0053] Figure 10The results showed that after 5 days of storage, the grapes wrapped in the product's plastic wrap exhibited significant oxidative deterioration, while the green grapes wrapped in the slow-release film with an essential oil characteristic component concentration of 50 μL / mL showed no significant oxidation even after 7 days.

[0054] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A sustained-release film material containing plant essential oils, characterized in that, The raw materials for preparing the sustained-release film material containing plant essential oils include the following components in the following mass-volume ratio: The ratio of sodium alginate, pectin, emulsifier, and plant essential oil is 1 g: 1 g: 0.04~1 mL: 0.25~10 mL.

2. The sustained-release film material containing plant essential oil according to claim 1, characterized in that, The emulsifier includes one of the following: glycerol, glyceryl monostearate, sodium lauryl sulfate, sodium dodecylbenzene sulfonate, Tween-80, polyoxyethylene ether, polyoxypropylene ether, ethylene oxide and propylene oxide block copolymer, polyol fatty acid ester, polyethylene glycol, starch, and polyoxyethylene polyoxypropylene ether triblock copolymer.

3. The sustained-release film material containing plant essential oil according to claim 1, characterized in that, The plant essential oil mentioned is eucalyptus essential oil.

4. A method for preparing a sustained-release film material containing plant essential oil as described in any one of claims 1 to 3, characterized in that, Includes the following steps: (1) Dissolve sodium alginate, pectin and emulsifier in water, heat and stir to obtain film-forming solution; (2) Add eucalyptus oil to the film-forming solution, stir and sonicate to obtain an emulsion; (3) The emulsion was left to stand and cure to obtain a slow-release film material containing plant essential oils.

5. The method for preparing the sustained-release film material containing plant essential oil according to claim 4, characterized in that, The volume ratio of the emulsifier to water in step (1) is 0.2~1:

100.

6. The method for preparing the sustained-release film material containing plant essential oil according to claim 4, characterized in that, In step (1), the heating and stirring temperature is 60~80 ℃ and the time is 6~7 h.

7. The method for preparing the sustained-release film material containing plant essential oil according to claim 4, characterized in that, The stirring temperature in step (2) is 25~55 ℃ and the time is 20~40 min; the ultrasonic temperature is 25~40 ℃ and the time is 1~3 h.

8. The method for preparing the sustained-release film material containing plant essential oil according to claim 4, characterized in that, The temperature for static curing in step (3) is 20~45 ℃ and the time is 24~48 h.

9. The use of a sustained-release film material containing plant essential oil as described in any one of claims 1 to 3, or a sustained-release film material containing plant essential oil prepared by the preparation method described in any one of claims 4 to 8, in the preparation of preservation and / or antibacterial products.

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