Sectional type temperature control column oven for liquid chromatography

Abstract

A sectional type temperature control column oven for liquid chromatography comprises an oven body, a fan and a chromatographic column are arranged in the oven body, an upper baffle and a lower baffle are arranged in the oven body, the upper baffle and the lower baffle are fixedly connected to form a partition to divide the interior of the oven body into two temperature control areas located on the two sides of the partition, and the chromatographic column is located in the temperature control areas. The temperature control device has the beneficial effects that different temperature control areas are divided by the partitions, and the partition temperature control function of the chromatographic column is realized by combining a plurality of groups of fans, so that the chromatographic column is positioned in different temperature environments on the two sides of the partitions.

Description

Technical Field

[0001] This invention relates to the field of liquid chromatography technology, and in particular to a segmented temperature-controlled column oven for liquid chromatography. Background Technology

[0002] As an important component of a liquid chromatograph, the column oven's main function is to control the temperature of the area (cavity) where the chromatographic column is located, ensuring that the column is under accurate and stable specified temperature conditions during the analysis process. This, in turn, ensures the repeatability of retention times in the liquid chromatography analysis results and the reproducibility of the method.

[0003] In liquid chromatography (LC), sample separation primarily relies on isocratic or gradient elution of the mobile phase. The type, ratio, and additives of the mobile phase are then adjusted based on the results to achieve separation. In addition, temperature significantly affects the separation efficiency of LC. Therefore, during the development of LC methods, adjusting the column temperature can improve resolution and achieve better separation of different substances.

[0004] However, more and more samples require liquid chromatography analysis, and the complexity of the samples has also increased significantly. The previous adjustments to the type and ratio of mobile phase are no longer suitable for the separation and analysis of all samples.

[0005] Liquid chromatography theory shows that changes in column temperature have a significant impact on resolution. Therefore, temperature can be considered an important parameter for adjusting resolution, offering new insights into liquid chromatography separation. Consequently, a segmented temperature-controlled column oven for liquid chromatography is needed. Summary of the Invention

[0006] The purpose of this invention is to solve the above-mentioned problems by designing a segmented temperature-controlled column oven for liquid chromatography. The specific design scheme is as follows:

[0007] A segmented temperature-controlled column oven for liquid chromatography includes a chamber, a fan and a chromatographic column inside the chamber, an upper baffle and a lower baffle inside the chamber, the upper baffle and the lower baffle being fixedly connected to form a partition that divides the chamber into two temperature-controlled zones located on both sides of the partition, the temperature-controlled zones being specifically Peltier temperature-controlled zones, and the chromatographic column being located within the temperature-controlled zones.

[0008] The lower baffle is mounted on the padding layer, and the top of the box is provided with a box cover. The box cover is rotatably connected to the box body. When the box cover is closed, the temperature control area is formed between the box cover and the padding layer by the inner wall of the box, the box cover, and the padding layer. An insulation layer can also be provided on the box cover.

[0009] The number of fans is multiple, and the multiple fans are distributed in a rectangular array in the vertical cross section along the axial direction of the chromatographic column inside the chamber. In the vertical direction of the rectangular array distribution, the multiple fans can increase the temperature regulation capability of corresponding positions in the same temperature control area. In the horizontal direction of the rectangular array distribution, the multiple fans can differentiate the temperature control of different positions in the same temperature control area.

[0010] The partition is inserted into a limiting groove located on the inner wall of the chamber. The limiting groove is used to fix the partition. There are multiple sets of limiting grooves. The multiple sets of limiting grooves are distributed in a linear array along the axial direction of the chromatographic column in the chamber. The multiple limiting grooves can fix the partition at different positions in the chamber, thereby dividing the interior of the chamber into different temperature control zones.

[0011] The number of the partitions is at least one. When the number of the partitions is multiple, they can be fixed by multiple limiting grooves, thereby dividing multiple temperature control zones.

[0012] The chromatographic column passes through the partition, the upper baffle and the lower baffle are inserted and connected, the upper baffle and the lower baffle are respectively provided with mutually fitting grooves and blocks, and the upper baffle or the lower baffle is provided with a groove for fixing the chromatographic column by interference fit.

[0013] The beneficial effects of the segmented temperature-controlled column oven for liquid chromatography obtained by the above-described technical solution of the present invention are as follows:

[0014] By dividing the column into different temperature control zones with partitions and combining multiple fans, the chromatographic column can be divided into zones for temperature control, allowing the column to be in different temperature environments on both sides of the partition. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the segmented temperature-controlled column oven for liquid chromatography described in this invention;

[0016] Figure 2 This is a top view schematic diagram of the segmented temperature-controlled column oven for liquid chromatography described in this invention;

[0017] Figure 3 This is a schematic diagram of the structure of the segmented temperature-controlled column oven for liquid chromatography described in this invention after the chamber is concealed.

[0018] Figure 4 This is a schematic diagram of the structure of the upper baffle described in this invention;

[0019] Figure 5 This is a schematic diagram of the structure of the lower baffle described in this invention;

[0020] Figure 6This is a schematic diagram of the structure of the partition described in this invention;

[0021] Figure 7 This is a schematic diagram of the structure in Embodiment 1 of the present invention for the fan to achieve zoned control of the temperature control area;

[0022] Figure 8 This is a schematic diagram of the structure of different temperature control zones divided by partitions in Embodiment 2 of the present invention;

[0023] Figure 9 This is a schematic diagram of the structure in Embodiment 3 of the present invention, which uses a fan and a baffle to comprehensively control the temperature control area.

[0024] In the diagram, 1. Chamber; 2. Fan; 3. Chromatographic column; 4. Upper baffle; 5. Lower baffle; 6. Temperature control zone; 7. Pad; 8. Chamber lid; 9. Insulation layer; 10. Divider; 11. Limiting groove; 12. Insert; 13. Insert; 14. Groove; T1, Temperature control zone corresponding to the first set of fans; T2, Temperature control zone corresponding to the second set of fans; T3, Temperature control zone corresponding to the third set of fans; T4, Temperature control zone corresponding to the fourth set of fans; Ta, The first temperature control zone divided by the divider. Tb, the second temperature control zone divided by the partition; Tc, the third temperature control zone divided by the partition; T1a, the temperature control zone in the first temperature control zone divided by the partition that is controlled by the first set of fans; T1b, the temperature control zone in the first temperature control zone divided by the partition that is controlled by the second set of fans; T2a, the temperature control zone in the first temperature control zone divided by the partition that is controlled by the first set of fans; T3a, the temperature control zone in the third temperature control zone divided by the partition that is controlled by the first set of fans. Detailed Implementation

[0025] The present invention will now be described in detail with reference to the accompanying drawings.

[0026] A segmented temperature-controlled column oven for liquid chromatography includes a chamber 1, a fan 2 and a chromatographic column 3 inside the chamber 1, and an upper baffle 4 and a lower baffle 5 inside the chamber 1. The upper baffle 4 and the lower baffle 5 are fixedly connected to form a partition 10, which divides the chamber 1 into two temperature-controlled zones 6 located on both sides of the partition 10. The temperature-controlled zones are specifically Peltier temperature-controlled zones, and the chromatographic column 3 is located within the temperature-controlled zone 6.

[0027] The lower baffle 5 is mounted on the padding layer 7. The top of the box body 1 is provided with a box cover 8. The box cover 8 is rotatably connected to the box body 1. When the box cover 8 is closed, the temperature control area 6 is formed between the box cover 8 and the padding layer 7 by the inner wall of the box body 1, the box cover 8, and the padding layer 7. An insulation layer 9 can also be provided on the box cover 8.

[0028] The number of fans 2 is multiple, and the multiple fans 2 are distributed in a rectangular array in the vertical cross section along the axial direction of the chromatographic column 3 inside the housing 1. In the vertical direction of the rectangular array distribution, the multiple fans 2 can increase the temperature regulation capability of the corresponding position in the same temperature control zone 6. In the horizontal direction of the rectangular array distribution, the multiple fans 2 can differentiate the temperature control of different positions in the same temperature control zone 6.

[0029] The partition 10 is inserted into the limiting groove 11 located on the inner wall of the housing 1. The limiting groove 11 is used to fix the partition 10. There are multiple sets of limiting grooves 11. The multiple sets of limiting grooves 11 are arranged in a linear array along the axial direction of the chromatographic column 3 in the housing 1. The multiple limiting grooves 11 can fix the partition 10 at different positions in the housing 1, thereby dividing the interior of the housing 1 into different temperature control zones 6.

[0030] The number of the partitions 10 is at least one. When the number of the partitions 10 is multiple, they can be fixed by multiple limiting grooves 11, thereby dividing multiple temperature control areas 6.

[0031] The chromatographic column 3 passes through the partition 10. The upper baffle 4 and the lower baffle 5 are inserted and connected. The upper baffle 4 and the lower baffle 5 are respectively provided with mutually fitting grooves 12 and inserts 13. The upper baffle 4 or the lower baffle 5 is provided with a groove 14 for fixing the chromatographic column 3 by interference fit.

[0032] Example 1: Temperature control of the same temperature control zone by multiple sets of fans

[0033] like Figure 7 As shown, Figure 7 Four sets of fans 2 are arranged horizontally in the middle. Each set of fans 2 independently controls the temperature control area 6 above it, corresponding to the four parts T1, T2, T3 and T4 respectively. Although they are in the same temperature control area 6, different temperature controls are achieved according to the different temperature control adjustments of the four sets of fans 2. However, there is heat exchange of air within the same temperature control area 6. This control is adapted to the temperature control required for a smooth temperature transition in existing liquid chromatography methods.

[0034] Example 2: Barriers and Control of Temperature Control Zones

[0035] like Figure 8 As shown, the partition 10 divides the temperature control area 6, blocking the heat exchange of air on both sides of the partition 10. At this time, there can be a large difference in temperature at Ta, Tb and Tc. This control is adapted to the temperature control requirements for rapid temperature changes in existing liquid chromatography methods.

[0036] Example 3: Integrated temperature control of the temperature control zone by the fan and baffles

[0037] like Figure 9 As shown, by flexibly utilizing the position and number of the baffles 10 and the number of fans 2 distributed vertically along the rectangular array, comprehensive control of the temperature control zone 6 can be achieved. At this time, the temperature difference between T1a and T1b is relatively smooth, while the temperature difference between T1b and T2a can be relatively large.

[0038] The above technical solutions only embody the preferred technical solutions of the present invention. Any modifications that may be made by those skilled in the art to certain parts thereof embody the principles of the present invention and fall within the protection scope of the present invention.

Claims

1. A segmented temperature-controlled column oven for liquid chromatography, comprising a chamber (1), wherein a fan (2) and a chromatographic column (3) are provided inside the chamber (1), characterized in that, The box (1) is provided with an upper baffle (4) and a lower baffle (5). The upper baffle (4) and the lower baffle (5) are fixedly connected to form a partition (10) which divides the box (1) into two temperature control zones (6) located on both sides of the partition (10). The chromatographic column (3) is located in the temperature control zone (6).

2. The segmented temperature-controlled column oven for liquid chromatography according to claim 1, characterized in that, The lower baffle (5) is mounted on the pad (7).

3. The segmented temperature-controlled column oven for liquid chromatography according to claim 1, characterized in that, The number of the fans (2) is multiple.

4. The segmented temperature-controlled column oven for liquid chromatography according to claim 1, characterized in that, The partition (10) is inserted into the limiting groove (11) located on the inner wall of the box (1).

5. The segmented temperature-controlled column oven for liquid chromatography according to claim 1, characterized in that... 。 6. The segmented temperature-controlled column oven for liquid chromatography according to claim 1, characterized in that, The chromatographic column (3) penetrates the partition (10).

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