Application of streptomyces albidoflavus strain SaTC2601 in promoting conversion of protaetia brevitarsis into straw

Abstract

The invention relates to application of a streptomyces albidoflavus strain SaTC2601 to promotion of straw conversion of protaetia brevitarsis. The streptomyces albidoflavus is found to be capable of promoting the straw conversion rate of the protaetia brevitarsis larvae and promoting the increase of the protaetia brevitarsis larvae after being used for treating the straws, so that not only is the absorption of straw non-point source pollution facilitated, but also the protein yield of the protaetia brevitarsis larvae can be increased by increasing the weight of the protaetia brevitarsis larvae; therefore, a channel which is more beneficial to guaranteeing the national grain safety is provided.

Description

Technical Field

[0001] This invention relates to the field of microorganisms, and in particular to a Streptomyces that promotes weight gain and / or promotes straw conversion in white-spotted beetles. Background Technology

[0002] With the continuous advancement of agricultural modernization, the issue of resource utilization of crop straw has become increasingly prominent. According to incomplete statistics, China produces more than 700 million tons of crop straw annually. How to scientifically and efficiently treat these agricultural wastes and realize their resource utilization has become an important issue for the sustainable development of agriculture.

[0003] White-spotted Flower Beetle ( Protaetia brevitarsis The larvae of the White Spotted Flower Chafer (WSFC) are saprophytic and do not harm plants. They are a resource insect that can convert agricultural waste into high-quality insect protein. They have been domesticated as "micro-livestock," and their dung has a good growth-promoting effect on plants, thus making them highly valuable. Summary of the Invention

[0004] This invention provides *Streptomyces microcyticum*. Streptomyces albidoflavus Application in promoting the conversion of straw by the white-spotted flower beetle and / or promoting the weight gain of the white-spotted flower beetle.

[0005] In one specific embodiment, the strain of *Streptomyces microcyticus* is strain SaTC2601.

[0006] In one specific embodiment, the white-spotted flower beetle is the larva of the white-spotted flower beetle.

[0007] In one specific embodiment, the straw is corn straw.

[0008] In one specific embodiment, the straw is treated with Streptomyces microcyticum strain SaTC2601 for 3 to 10 days before being fed to the white-spotted beetle, for example, for 5 to 7 days.

[0009] In one specific embodiment, the straw is treated with Streptomyces microcyticum SaTC2601 strain at a temperature of 25 to 37 degrees Celsius.

[0010] In one specific embodiment, the straw is treated with Streptomyces microcyticum SaTC2601 strain at a temperature of 28 to 30 degrees Celsius.

[0011] The beneficial effects of the present application: the present application finds that the use of micro-white yellow Streptomyces can promote the conversion rate of straw by star flower beetle larvae and promote the increase of star flower beetle larvae, which not only helps to consume straw non-point source pollution, but also increases the protein yield of star flower beetle larvae, thereby providing a more favorable channel to ensure national food security. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 The photo shows the corn straw treated with SaTC2601 for 3 days. DETAILED DESCRIPTION

[0013] The above description of the present application is further detailed in the form of preferred embodiments, but it does not constitute a limitation on the present application.

[0014] Unless otherwise specified, the reagents and the like in the embodiments of the present application can be purchased through commercial channels.

[0015] Micro-white yellow Streptomyces used in the present application Streptomyces albidoflavus The SaTC2601 strain has been first disclosed in CN202510827215.9, which is preserved in the China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 33741, the preservation date of March 7, 2025, and the preservation address of No. 3, Institute of Microbiology, Chinese Academy of Sciences, Beijing City, Chaoyang District, Beichen West Road 1st Courtyard 3rd. 1. Preparation of micro-white yellow Streptomyces spores

[0016] Worm manure soluble extract: take 100 g of dried star flower beetle (WSFC) larvae worm manure (water content below 3%) and add 1 L of conical flask, add water to 1 L, extract at 80 degrees Celsius for 2 hours, centrifuge to remove insoluble matter, obtain water extract, spray dry the water extract to obtain water extract dry powder.

[0017] WSFC worm manure water extract solid medium: take 10 g of WSFC larvae worm manure water extract dry powder, add 15 g of agar powder, add water to 1 L, sterilize at 121 degrees Celsius for 20 min, to prepare 1% of WSFC worm manure water extract solid medium. Pour the above 1% of WSFC worm manure water extract solid medium into a 90 mm diameter culture dish, 20 ml per culture dish, to prepare WSFC worm manure water extract solid medium.

[0018] The WSFC worm manure water extract solid medium was used to expand the strains of Microdochium albocinereum SaTC2601 and X14, i.e. the spores were produced by culturing the strains on the WSFC worm manure water extract solid medium at 28 degrees Celsius for 7 days. The spores were collected by suspending with sterile water to prepare a spore suspension of 1 x 108 CFU / ml for each strain. 2. Treatment of corn stalks

[0019] After the corn was harvested, the corn stalks were air-dried to have a water content of less than 3%, and were extruded and pelletized into stalk feed pellets (the mass met the requirements of the national standard "GB / T 35835-2018 Corn Stalk Pellets"). 150 ml of the spore suspension of SaTC2601 or X14 described above was added to 100 g of the stalk feed pellets, respectively, as the Microdochium albocinereum SaTC2601 treatment group and the Microdochium albocinereum X14 treatment group; a commercially available stalk decomposing agent was configured into a 150 ml live bacteria suspension of 1 x 108 CFU / ml, and was added to 100 g of the stalk feed pellets as the commercially available stalk decomposing agent treatment group; 150 ml of sterile water was added to 100 g of the stalk feed pellets as the control group. The stalks of each treatment group and the control group were placed in an incubator at 30 degrees Celsius and were allowed to ferment for 3 days, and the results are shown in Table 1. Figure 1 .

[0020] As can be seen from Table 1, actinomycete spores and actinomycete mycelia were observed on the stalks of the treatment groups. Figure 1 3. Effect of SaTC2601 on the body weight of Polyphylla laticornis larvae and promotion of stalk conversion by the larvae 60 g of the stalk feed pellets of each treatment group and the control group that were fermented for 3 days were taken and were placed in insect rearing boxes. The SaTC2601 treatment group, the X14 treatment group, and the commercially available stalk decomposing agent treatment group all had white actinomycete mycelia and spores. The body weight of the Polyphylla laticornis third instar larvae was measured and recorded, and larvae with equivalent body weight were selected and were placed in the insect rearing boxes, with one insect rearing box as one replicate, 10 larvae per replicate, and 3 replicates for each treatment group. The larvae were reared in an incubator at 30 degrees Celsius for 2 days. The larvae, feces, and remaining stalk feed pellets of each group were collected and were recorded, and the weight gain of the larvae, the intake (the mass of the initial stalk feed pellets minus the mass of the remaining stalk feed pellets after 2 days of feeding) of each insect rearing box, the stalk conversion rate, and the stalk conversion efficiency were calculated. The stalk conversion rate was characterized by the intake per kilogram of Polyphylla laticornis larvae per day, as shown in formula (1); the stalk conversion efficiency was the efficiency of converting the consumed stalks into the weight of the insects, which was calculated by dividing the weight gain of the larvae by the weight of the consumed stalks, as shown in formula (2). The difference between the SaTC2601 treatment group and the control group was analyzed by t-test. The results are shown in Table 1.

[0021]

[0022] ​Conversion rate of straw = (intake amount / intake time) / initial weight of insect × 1000g Formula (1).

[0023] Conversion efficiency of straw = (weight gain of insect / intake amount) × 100% Formula (2).

[0024] Table 1

[0025] The results of Table 1 show that the conversion rate of straw and the conversion efficiency of straw of the SaTC2601 treatment group, the X14 treatment group and the commercially available straw decomposing agent treatment group are significantly higher than those of the control group (p<0.01), indicating that the addition of microbial inoculants can promote the conversion of straw by Anomala daimiana; compared with the X14 treatment group, the conversion rate of straw and the conversion efficiency of straw of the commercially available straw decomposing agent treatment group are significantly higher than those of the X14 treatment group (p<0.01), indicating that although the conventional X14 can promote the conversion, the effect is not as good as the synergistic effect of multiple strains of the market sold compound microbial inoculants. The conversion rate of straw of the SaTC2601 treatment group is 417.93 g, which is significantly higher than that of the control group 342.39 g (p<0.01), which is 1.22 times that of the control group, indicating that the larvae prefer to feed on the straw treated by SaTC2601, which may be related to the special smell produced by the fermentation of the strain; the weight gain of the insect in the SaTC2601 treatment group is 2.66 g, which is significantly higher than that in other treatment groups (p<0.01); the conversion efficiency of straw in the SaTC2601 treatment group is 20.90%, which is significantly higher than that in other treatment groups (p<0.01), indicating that the fermentation of the strain can produce more absorbable nutrients.

Claims

1. Streptomyces whitworthii Streptomyces albidoflavus Application in promoting the conversion of straw by the white-spotted flower beetle and / or promoting the weight gain of the white-spotted flower beetle.

2. The application according to claim 1, characterized in that, The strain of Streptomyces microcytogenes is SaTC2601.

3. The application according to claim 1, characterized in that, The white-spotted flower beetle is the larva of the white-spotted flower beetle.

4. The application according to claim 1, characterized in that, The straw in question is corn stalks.

5. The application according to claim 2, characterized in that, The straw was treated with Streptomyces microcyticum strain SaTC2601 for 3 to 10 days before being fed to the white-spotted flower beetle.

6. The application according to claim 5, characterized in that, The straw was treated with Streptomyces microcyticum strain SaTC2601 at a temperature of 25 to 37 degrees Celsius.

7. The application according to claim 5, characterized in that, The straw was treated with Streptomyces microcyticum strain SaTC2601 at a temperature of 28 to 30 degrees Celsius.

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