Liquid functional fragrance capable of resisting fatigue and improving immunity and preparation method of liquid functional fragrance
By using composite essential oil nano-microcapsule technology, the problems of volatility and stability of essential oil fragrances have been solved, achieving sustained release and long-lasting effects of essential oils, and enhancing anti-fatigue and immunity effects.
Patent Information
- Application Number
- CN202511510871.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-22
- Publication Date
- 2025-12-16
AI Technical Summary
Existing essential oil fragrances are highly volatile and have poor stability during storage and use, resulting in reduced durability and efficacy. They are also prone to oxidation and decomposition, increasing usage costs.
Using composite essential oil nano-microcapsule technology, modified soy protein isolate and modified chitosan are used as wall materials to protect the active ingredients of essential oils. Combined with propylene glycol aqueous solution and preservatives, a liquid functional fragrance that combats fatigue and enhances immunity is prepared.
It extends the shelf life of essential oils, achieves a slow-release effect, reduces the need for frequent replenishment, lowers usage costs, and maintains the longevity and therapeutic effects of the fragrance.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of functional fragrance, in particular to an anti-fatigue and immunity-enhancing liquid functional fragrance and a preparation method thereof. BACKGROUND
[0002] Normal sleep plays a crucial role in the formation of learning and memory. Long-term reduction in sleep time and decline in sleep quality, i.e. sleep deprivation, can trigger a series of functional changes, including mood, learning and memory, immunity, etc., especially cognitive function is significantly affected. Specifically, learning and memory ability decreases, reaction is slow, attention is scattered, and directional and spatial disorders occur, etc. During long-term work, the decline in alertness caused by sleep deprivation is particularly prominent, which not only affects work efficiency, but also may increase the risk of accidents. Therefore, it is of great significance to find effective intervention means to prevent and treat cognitive impairment caused by sleep deprivation.
[0003] Aromatherapy, as a non-drug intervention means, combines the dual functions of art and treatment, and is a holistic treatment-like method that returns to nature. It comprehensively considers the needs of human physiology, reason and spirit, and achieves the purpose of relieving mental stress, treating diseases and promoting human health by using essential oils extracted from plants as a medium. The aromatic substances or essential oils used in aromatherapy have been reported to have a wake-promoting effect. Essential oils have good curative effect and small side effects, and are widely used to refresh the mind, prolong daily wakefulness time.
[0004] Although essential oils have many advantages in aromatherapy, they also have some defects: essential oils usually have high volatility, and in the absence of packaging, they are easy to volatilize quickly during storage and use. This leads to poor persistence of the fragrance, which requires frequent replenishment, increasing the cost of use; the active ingredients in essential oils are easy to oxidize, decompose and react under the influence of environmental factors such as light and oxygen, reducing their stability and efficacy. This not only affects the shelf life of the product, but also may greatly reduce the efficacy of essential oils. SUMMARY
[0005] In order to improve the therapeutic effect of functional essential oils in anti-fatigue and immunity enhancement, the present application provides an anti-fatigue and immunity-enhancing liquid functional fragrance and a preparation method thereof.
[0006] The anti-fatigue and immunity-enhancing liquid functional fragrance provided by the present application adopts the following technical solution: An anti-fatigue and immunity-enhancing liquid functional fragrance, the raw materials include 10-30 parts of composite essential oil nano-microcapsules, 30-50 parts of base oil, 0.1-0.5 parts of preservative, and 20-40 parts of solvent by weight; The complex essential oil nanocapsule takes the complex essential oil as the core material and takes the modified soybean protein isolate and the modified chitosan as the wall material. The complex essential oil includes 10-14% angelica essential oil, 8-12% mint essential oil, 12-16% rosemary essential oil, 8-12% lemon essential oil, 4-8% eucalyptus oil, 7-11% camellia essential oil, 10-14% nutmeg essential oil, 10-14% rhodiola essential oil, 8-12% ginseng essential oil, 2.5-3.5% vitamin E oil, and 1.5-6.5% orange flower essential oil in terms of mass percentage.
[0007] Preferably, the complex essential oil includes 14% angelica essential oil, 10% mint essential oil, 12% rosemary essential oil, 6% lemon essential oil, 8% eucalyptus oil, 9% camellia essential oil, 14% nutmeg essential oil, 10% rhodiola essential oil, 10% ginseng essential oil, 3% vitamin E oil, and 4% orange flower essential oil in terms of mass percentage.
[0008] Preferably, the base oil includes sweet almond oil, jojoba oil, and grape seed oil, and the mass ratio of the sweet almond oil, the jojoba oil, and the grape seed oil is (1-2):(2-3):(4-6).
[0009] Preferably, the solvent is a 30-70% propylene glycol aqueous solution in terms of mass fraction.
[0010] Preferably, the preparation method of the modified soybean protein isolate includes the following steps. The soybean protein isolate is added into water and uniformly mixed to obtain a 4-5 mg / mL soybean protein isolate solution. After the pH of the solution is adjusted to 11-12, the solution is immediately subjected to ultrasonic treatment. Subsequently, the solution is subjected to constant temperature stirring at 10-20 ℃ for 30-40 min. Then, the pH is adjusted to neutral, and the solution is subjected to magnetic stirring in an ice water bath for 15-20 min to obtain the modified soybean protein isolate.
[0011] Preferably, the ultrasonic treatment is performed at a frequency of 20-22 kHz for 5-7 min.
[0012] Preferably, the modified chitosan is prepared from the following raw materials: 1-2 parts of chitosan, 40-80 parts of acetic acid solution, 0.05-0.1 parts of ascorbic acid, 2-4 parts of hydrogen peroxide solution, 0.1-0.3 parts of protocatechuic acid, and 20-40 parts of sodium acetate buffer.
[0013] Preferably, the preparation method of the modified chitosan includes the following steps. The modified chitosan is obtained by dissolving 1-2 parts of chitosan in 40-80 parts of acetic acid solution overnight, adjusting the pH to 5-6, then adding 0.05-0.1 parts of ascorbic acid and 2-4 parts of hydrogen peroxide solution mixture to the solution under a nitrogen atmosphere, incubating at 40-50 DEG C for 1-2 h, then adding 0.1-0.3 parts of protocatechuic acid, incubating at 40-50 DEG C under a nitrogen atmosphere for 24-30 h, using a dialysis bag to dialyze the obtained mixture with 20-40 parts of sodium acetate buffer at 4-6 DEG C for 12-15 h, replacing the dialysate every 4-6 h, and freeze-drying after dialysis is completed.
[0014] The application provides a preparation method of an anti-fatigue and immunity-enhancing liquid functional fragrance. The application provides a preparation method of an anti-fatigue and immunity-enhancing liquid functional fragrance. S1. Preparing composite essential oil nanocapsules; S2. Mixing the prepared composite essential oil nanocapsules with base oil, magnetically stirring at 300-500 rpm for 30-50 min to fully disperse the nanocapsules in the base oil, mixing the obtained mixture with a propylene glycol aqueous solution, and continuing to stir for 20-30 min, and finally adding a preservative, and stirring for 10-20 min to obtain the anti-fatigue and immunity-enhancing liquid functional fragrance.
[0015] Preferably, the preparation method of the composite essential oil nanocapsules comprises the following steps. Preferably, the preparation method of the composite essential oil nanocapsules comprises the following steps. Preferably, the preparation method of the composite essential oil nanocapsules comprises the following steps.
[0016] Preferably, the preparation method of the composite essential oil nanocapsules comprises the following steps. 1. The application provides an anti-fatigue and immunity-enhancing liquid functional fragrance, and the composite essential oil is compounded from angelica essential oil, peppermint essential oil, rosemary essential oil, lemon essential oil, eucalyptus oil, camellia essential oil, nutmeg essential oil, rhodiola essential oil, ginseng essential oil, vitamin E oil and orange flower essential oil. Angelica essential oil has the effect of dispelling wind and cold, unblocking channels and relieving pain, and can relieve headaches and nasal congestion caused by fatigue, thereby reducing fatigue and improving mental state.
[0017] Peppermint essential oil contains menthol and other components, which have the effects of refreshing and relieving fatigue. It can stimulate the nervous system, making people feel refreshed, improve concentration and alertness, and effectively relieve fatigue.
[0018] Rosemary essential oil has anti-inflammatory and anti-fatigue effects. It can promote blood circulation and improve brain oxygen supply, thereby reducing fatigue, improving memory and attention, and enhancing cognitive function.
[0019] Lemon essential oil is rich in limonene and other components, which have the effects of refreshing and enhancing immunity. It can improve mood, reduce stress, and enhance the body's antioxidant capacity to help resist fatigue.
[0020] Eucalyptus oil contains eucalyptol and other components, which have a refreshing eucalyptus aroma and can refresh the mind and relieve fatigue. It also has antibacterial effects and can enhance the resistance of the respiratory tract and improve immunity.
[0021] Camellia essential oil has antibacterial, anti-inflammatory and soothing skin effects, and has certain refreshing effects, which can help improve mental state, reduce fatigue, and enhance the body's overall resistance.
[0022] Nutmeg essential oil has certain sedative effects, which can help relieve stress and improve sleep quality, thereby indirectly improving the body's immunity and anti-fatigue ability.
[0023] Red ginseng essential oil has anti-fatigue and anti-hypoxia effects. It can enhance the body's endurance and adaptability, help relieve fatigue, and improve the body's immunity and resistance to disease.
[0024] Ginseng essential oil has the effects of tonifying qi and enhancing immunity. It can regulate the body's physiological functions, improve the body's anti-fatigue ability and immunity, and make people energetic.
[0025] Vitamin E oil has antioxidant effects and can improve immunity. It can protect cells from damage by free radicals and enhance the body's antioxidant defense system, thereby reducing fatigue and improving immunity.
[0026] Orange flower essential oil has the effects of soothing emotions, reducing stress and improving sleep. Good mood and sleep quality can help reduce fatigue, improve immunity and enhance the body's overall health.
[0027] The proportions of various essential oils are carefully designed to ensure that the effective components of each essential oil can play their full role, while avoiding the irritation or other adverse reactions that may be caused by excessive concentration of a single essential oil, making the entire fragrance formula more balanced and gentle.
[0028] 2. This application uses compound essential oil as the core material and modified soy protein isolate and modified chitosan as wall materials to prepare compound essential oil nanocapsules. The wall material of the nanocapsules can effectively protect the active ingredients in the essential oil and prevent them from being oxidized and decomposed under the influence of environmental factors such as light and oxygen, thereby extending the shelf life of the essential oil and maintaining its efficacy. In addition, the nano-microencapsulation of essential oil can achieve a sustained-release effect, allowing the essential oil components to be released gradually, prolonging the fragrance's longevity, reducing the need for frequent replenishment, and lowering the cost of use. Detailed Implementation
[0029] The present application will be further described in detail below with reference to the embodiments.
[0030] The chemical reagents used in the preparation examples, embodiments, and comparative examples provided in this invention are all commercially available products.
[0031] Preparation Example 1: Preparation of Modified Soy Protein Isolate Preparation Example 1.1 Add 40 mg of soy protein isolate to 10 mL of water and mix well to obtain a soy protein isolate solution. Adjust the pH to 11 with 2 mol / L sodium hydroxide solution and then sonicate at 20 kHz for 5 min. Then stir in a 10℃ constant temperature magnetic stirring water bath for 30 min. Finally, adjust the pH to neutral and stir magnetically in an ice water bath for 15 min to obtain modified soy protein isolate.
[0032] Preparation Example 1.2 Add 45 mg of soy protein isolate to 10 mL of water and mix well to obtain a soy protein isolate solution. Adjust the pH to 11.5 with 2 mol / L sodium hydroxide solution and then sonicate at 21 kHz for 6 min. Then stir in a 15℃ constant temperature magnetic stirring water bath for 35 min. Finally, adjust the pH to neutral and stir magnetically in an ice water bath for 17 min to obtain modified soy protein isolate.
[0033] Preparation Example 1.3 Add 50 mg of soy protein isolate to 10 mL of water and mix well to obtain a soy protein isolate solution. Adjust the pH to 12 with 2 mol / L sodium hydroxide solution and then sonicate at 22 kHz for 7 min. Then stir in a 20℃ constant temperature magnetic stirring water bath for 40 min. Finally, adjust the pH to neutral and stir magnetically in an ice water bath for 20 min to obtain modified soy protein isolate.
[0034] Preparation of modified chitosan Preparation Example 2.1 After 1 g of chitosan was dissolved in 40 g of 1% acetic acid solution overnight, the pH was adjusted to 5 with 1 mol / L sodium hydroxide solution; then, under a nitrogen atmosphere, a mixture of 0.05 g of ascorbic acid and 2 g of 0.5 mol / L hydrogen peroxide solution was added to the solution, which was incubated in a constant temperature oscillation shaker water bath at 40°C for 1 h; then, 0.1 g of protocatechuic acid was added, and the mixture was incubated at 40°C under a nitrogen atmosphere for 24 h; the resulting mixture was dialyzed against 20 g of 0.1 mol / L sodium acetate buffer (pH 5.0) at 4°C for 12 h using a dialysis bag (molecular weight cutoff: 8-14 kDa), and the dialysis solution was changed every 4 h; after dialysis was completed, the modified chitosan was obtained by freeze-drying.
[0035] Preparation Example 2.2 After 1.5 g of chitosan was dissolved in 60 g of 1% acetic acid solution overnight, the pH was adjusted to 5.5 with 1 mol / L sodium hydroxide solution; then, under a nitrogen atmosphere, a mixture of 0.075 g of ascorbic acid and 3 g of 0.5 mol / L hydrogen peroxide solution was added to the solution, which was incubated in a constant temperature oscillation shaker water bath at 45°C for 1.5 h; then, 0.2 g of protocatechuic acid was added, and the mixture was incubated at 45°C under a nitrogen atmosphere for 27 h; the resulting mixture was dialyzed against 30 g of 0.1 mol / L sodium acetate buffer (pH 5.0) at 5°C for 13.5 h using a dialysis bag (molecular weight cutoff: 8-14 kDa), and the dialysis solution was changed every 5 h; after dialysis was completed, the modified chitosan was obtained by freeze-drying.
[0036] Preparation Example 2.3 After 2 g of chitosan was dissolved in 80 g of 1% acetic acid solution overnight, the pH was adjusted to 6 with 1 mol / L sodium hydroxide solution; then, under a nitrogen atmosphere, a mixture of 0.1 g of ascorbic acid and 4 g of 0.5 mol / L hydrogen peroxide solution was added to the solution, which was incubated in a constant temperature oscillation shaker water bath at 50°C for 2 h; then, 0.3 g of protocatechuic acid was added, and the mixture was incubated at 50°C under a nitrogen atmosphere for 30 h; the resulting mixture was dialyzed against 40 g of 0.1 mol / L sodium acetate buffer (pH 5.0) at 6°C for 15 h using a dialysis bag (molecular weight cutoff: 8-14 kDa), and the dialysis solution was changed every 6 h; after dialysis was completed, the modified chitosan was obtained by freeze-drying.
[0037] Example 1 S1. To 20 g of modified soybean protein isolate solution prepared by Preparation Example 1.1, add 13 g of compound essential oil, then drop 32 g of modified chitosan prepared by Preparation Example 2.1 to obtain a mixed solution; homogenize the mixed solution at 10000 rpm for 3 min, adjust the pH of the mixed solution to 6 with 2 mol / L sodium hydroxide solution, then stir at 50℃, 300 rpm for 30 min for complex coacervation; then take out and quickly cool to 10℃ with ice water for 30 min to end the reaction; adjust the pH of the solution to 5 with 10% acetic acid, add 20 g of glutamine transaminase, and magnetically stir at 10℃ for 3 h to solidify, then centrifuge to obtain wet capsules; precool the wet capsules for 24 h, then freeze-dry for 48 h to obtain compound essential oil nano-microcapsules; S2. Mix 10 g of prepared compound essential oil nano-microcapsules with 30 g of base oil, magnetically stir at 300 rpm for 30 min to fully disperse the microcapsules in the base oil; mix the obtained mixture with 20 g of propylene glycol aqueous solution, continue to stir for 20 min; finally add 0.1 g of preservative, stir for 10 min to obtain an anti-fatigue and immunity-enhancing liquid functional fragrance; The compound essential oil comprises 12% Angelica dahurica essential oil, 8% mint essential oil, 16% rosemary essential oil, 8% lemon essential oil, 6% eucalyptus oil, 7% camellia essential oil, 12% nutmeg essential oil, 14% Rhodiola rosea essential oil, 8% ginseng essential oil, 2.5% vitamin E oil, and 6.5% orange flower essential oil, by mass percentage; The base oil comprises sweet almond oil, jojoba oil, and grape seed oil, in a mass ratio of 1:2:4; The solvent is a 30% propylene glycol aqueous solution by mass fraction; The preservative is phenoxyethanol.
[0038] Example 2 S1. To 20 g of modified soybean protein isolate solution prepared by Preparation Example 1.1, add 13 g of compound essential oil, then drop 32 g of modified chitosan prepared by Preparation Example 2.1 to obtain a mixed solution; homogenize the mixed solution at 10000 rpm for 3 min, adjust the pH of the mixed solution to 6 with 2 mol / L sodium hydroxide solution, then stir at 50℃, 300 rpm for 30 min for complex coacervation; then take out and quickly cool to 10℃ with ice water for 30 min to end the reaction; adjust the pH of the solution to 5 with 10% acetic acid, add 20 g of glutamine transaminase, and magnetically stir at 10℃ for 3 h to solidify, then centrifuge to obtain wet capsules; precool the wet capsules for 24 h, then freeze-dry for 48 h to obtain compound essential oil nano-microcapsules; S2. 20 g of the prepared composite essential oil nano-microcapsules were mixed with 40 g of base oil, and magnetic stirring was performed at 400 rpm for 40 min to fully disperse the microcapsules in the base oil; the obtained mixture was mixed with 30 g of propylene glycol aqueous solution, and stirring was continued for 25 min; finally, 0.3 g of preservative was added, and stirring was performed for 15 min to obtain an anti-fatigue and immunity-enhancing liquid functional fragrance; The composite essential oil comprises 12% angelica essential oil, 8% mint essential oil, 16% rosemary essential oil, 8% lemon essential oil, 6% eucalyptus oil, 7% camellia essential oil, 12% nutmeg essential oil, 14% rhodiola essential oil, 8% ginseng essential oil, 2.5% vitamin E oil, and 6.5% orange flower essential oil by mass percentage; The base oil comprises sweet almond oil, jojoba oil, and grape seed oil at a mass ratio of 1.5:2.5:5; The solvent is a propylene glycol aqueous solution with a mass fraction of 50%; The preservative is phenoxyethanol.
[0039] Example 3 S1. After 13 g of composite essential oil was added to 20 g of the modified soybean protein isolate solution prepared in Preparation Example 1.1, 32 g of the modified chitosan prepared in Preparation Example 2.1 was added dropwise to obtain a mixed solution; the mixed solution was homogenized at 10,000 rpm for 3 min, and the pH of the mixed solution was adjusted to 6 using a 2 mol / L sodium hydroxide solution, followed by stirring at 50°C and 300 rpm for 30 min for complex coacervation; then, the solution was taken out and rapidly cooled to 10°C using ice water for 30 min to end the reaction; the system pH was then adjusted to 5 using 10% acetic acid, and 20 g of glutamine transaminase was added, followed by magnetic stirring solidification at 10°C for 3 h, and then centrifugation to obtain wet capsules; the wet capsules were pre-cooled for 24 h, and then freeze-dried for 48 h to obtain composite essential oil nano-microcapsules; S2. 30 g of the prepared composite essential oil nano-microcapsules were mixed with 50 g of base oil, and magnetic stirring was performed at 500 rpm for 50 min to fully disperse the microcapsules in the base oil; the obtained mixture was mixed with 40 g of propylene glycol aqueous solution, and stirring was continued for 30 min; finally, 0.5 g of preservative was added, and stirring was performed for 20 min to obtain an anti-fatigue and immunity-enhancing liquid functional fragrance; The composite essential oil comprises 12% angelica essential oil, 8% mint essential oil, 16% rosemary essential oil, 8% lemon essential oil, 6% eucalyptus oil, 7% camellia essential oil, 12% nutmeg essential oil, 14% rhodiola essential oil, 8% ginseng essential oil, 2.5% vitamin E oil, and 6.5% orange flower essential oil by mass percentage; The base oil comprises sweet almond oil, jojoba oil, and grape seed oil at a mass ratio of 2:3:6; The solvent is a propylene glycol aqueous solution with a mass fraction of 70%; The preservative is phenoxyethanol.
[0040] Example 4 S1. After adding 19.5g of compound essential oil to 30g of modified soybean protein isolate solution prepared in Preparation Example 1.1, 48g of modified chitosan prepared in Preparation Example 2.1 was added dropwise to obtain a mixed solution; the mixed solution was homogenized at 11000rpm for 3.5min, the pH of the mixed solution was adjusted to 6.5 with 2mol / L sodium hydroxide solution, and then composite coacervation was carried out at 55℃ and 350rpm for 40min; then it was taken out and rapidly cooled to 13℃ with ice water for 35min to end the reaction; then the solution was adjusted to pH 5.5 with 10% acetic acid, and 30g of glutamine transaminase was added, and after magnetic stirring solidification at 13℃ for 4h, wet capsules were obtained by centrifugation; the wet capsules were pre-cooled for 27h and then freeze-dried for 54h to obtain compound essential oil nanocapsules; S2. 10g of prepared compound essential oil nanocapsules were mixed with 30g of base oil, and the mixture was stirred at 300rpm for 30min to fully disperse the nanocapsules in the base oil; the obtained mixture was mixed with 20g of propylene glycol aqueous solution and continued to stir for 20min; finally, 0.1g of preservative was added and stirred for 10min to obtain an anti-fatigue and immunity-enhancing liquid functional fragrance; The compound essential oil includes 12% angelica essential oil, 8% mint essential oil, 16% rosemary essential oil, 8% lemon essential oil, 6% eucalyptus oil, 7% camellia oil, 12% nutmeg essential oil, 14% rhodiola essential oil, 8% ginseng essential oil, 2.5% vitamin E oil, and 6.5% orange flower essential oil; The base oil includes sweet almond oil, jojoba oil, and grape seed oil, and the mass ratio is 1:2:4; The solvent is 30% propylene glycol aqueous solution by mass fraction; The preservative is phenoxyethanol.
[0041] Example 5 S1. After adding 26g of compound essential oil to 40g of modified soybean protein isolate solution prepared in Preparation Example 1.1, 64g of modified chitosan prepared in Preparation Example 2.1 was added dropwise to obtain a mixed solution; the mixed solution was homogenized at 12000rpm for 4min, the pH of the mixed solution was adjusted to 7 with 2mol / L sodium hydroxide solution, and then composite coacervation was carried out at 60℃ and 400rpm for 40min; then it was taken out and rapidly cooled to 15℃ with ice water for 40min to end the reaction; then the solution was adjusted to pH 6 with 10% acetic acid, and 40g of glutamine transaminase was added, and after magnetic stirring solidification at 15℃ for 5h, wet capsules were obtained by centrifugation; the wet capsules were pre-cooled for 30h and then freeze-dried for 60h to obtain compound essential oil nanocapsules; S2. 10 g of the prepared composite essential oil nanocapsules were mixed with 30 g of base oil, and magnetic stirring was performed at 300 rpm for 30 min to fully disperse the nanocapsules in the base oil; the obtained mixture was mixed with 20 g of a propylene glycol aqueous solution, and stirring was continued for 20 min; finally, 0.1 g of a preservative was added, and stirring was performed for 10 min to obtain an anti-fatigue and immunity-enhancing liquid functional fragrance; The composite essential oil comprises, by mass percentage, 12% angelica essential oil, 8% mint essential oil, 16% rosemary essential oil, 8% lemon essential oil, 6% eucalyptus oil, 7% camellia essential oil, 12% nutmeg essential oil, 14% rhodiola essential oil, 8% ginseng essential oil, 2.5% vitamin E oil, and 6.5% orange flower essential oil. The base oil comprises sweet almond oil, jojoba oil, and grape seed oil at a mass ratio of 1:2:4. The solvent is a propylene glycol aqueous solution with a mass fraction of 30%. The preservative is phenoxyethanol.
[0042] Example 6 Example 6 differs from Example 1 in that the composite essential oil in Example 6 comprises, by mass percentage, 14% angelica essential oil, 10% mint essential oil, 12% rosemary essential oil, 6% lemon essential oil, 8% eucalyptus oil, 9% camellia essential oil, 14% nutmeg essential oil, 10% rhodiola essential oil, 10% ginseng essential oil, 3% vitamin E oil, and 4% orange flower essential oil.
[0043] Example 7 Example 7 differs from Example 1 in that the composite essential oil in Example 7 comprises, by mass percentage, 10% angelica essential oil, 12% mint essential oil, 14% rosemary essential oil, 4% lemon essential oil, 10% eucalyptus oil, 11% camellia essential oil, 10% nutmeg essential oil, 12% rhodiola essential oil, 12% ginseng essential oil, 3.5% vitamin E oil, and 1.5% orange flower essential oil.
[0044] Example 8 Example 8 differs from Example 1 in that the modified soybean protein isolate used in Example 8 is prepared according to Preparation Example 1.2, and the modified chitosan used in Example 8 is prepared according to Preparation Example 2.2.
[0045] Example 9 Example 9 differs from Example 1 in that the modified soybean protein isolate used in Example 9 is prepared according to Preparation Example 1.3, and the modified chitosan used in Example 9 is prepared according to Preparation Example 2.3.
[0046] Comparative Example 1 Comparative Example 1 differs from Example 1 in that the soybean protein isolate used in Comparative Example 1 is not modified.
[0047] Comparative Example 2 Comparative Example 2 differs from Example 1 in that the chitosan employed in Comparative Example 2 is not modified.
[0048] Performance detection test Antioxidant activity test DPPH: A certain amount of DPPH solid powder was weighed and configured into a 0.1 mM DPPH-ethanol solution; the liquid functional fragrance obtained from Examples 1-9, Comparative Examples 1-2 and the control group was mixed with the DPPH reagent (0.1 mol / L) in a 96-well plate, after shaking, the absorbance value was measured at a wavelength of 517 nm after 60 min of dark standing. The results are shown in Table 1.
[0049] ABTS: 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid and sodium persulfate were weighed and added to anhydrous ethanol solution to fully dissolve, and then diluted to 100 mL, and then reacted for 16 h at room temperature in the dark to obtain an ABTS radical cation solution, and the solution was diluted with anhydrous ethanol to an absorbance of 0.700±0.01 at a wavelength of 734 nm as the ABTS reagent. 200 L of the ABTS reagent and the liquid functional fragrance obtained from Examples 1-9, Comparative Examples 1-2 and the control group were mixed and reacted at 25°C for 15 min, and the absorbance value was measured at a wavelength of 734 nm. The results are shown in Table 1.
[0050] Control group: The control group was set to only contain the wall material of the nanocapsule, and did not contain essential oil components.
[0051] The specific test results are as follows: Table 1 Antioxidant activity test results
[0052] As can be seen from the test results in Table 1, the anti-fatigue and immunity-enhancing liquid functional fragrance provided by the present application has excellent antioxidant performance, and when the compound essential oil includes 14% white privet essential oil, 10% mint essential oil, 12% rosemary essential oil, 6% lemon essential oil, 8% eucalyptus oil, 9% camellia oil, 14% nutmeg essential oil, 10% rhodiola essential oil, 10% ginseng essential oil, 3% vitamin E oil, and 4% orange flower essential oil by mass percentage, the DPPH and ABTS clearance rates are the largest.
[0053] II. Anti-fatigue activity test Twenty male ICR mice (6 weeks old, 22+2g) were housed in a laboratory with constant temperature (25±1℃), timed air exchange, and appropriate lighting (every 12 hours), and were provided with food and water. After a dry week of acclimatization, the mice were divided into two groups: a control group and an experimental group. The control group received ethanol solution instilled into their nostrils, while the experimental group received the anti-fatigue and immune-enhancing liquid fragrance obtained in Example 1 in their nostrils. Twenty-one days after administration, the mice underwent a weight-bearing swimming test, resulting in death from CO2 asphyxiation. Dissection was performed, and the kidneys and liver were weighed. The results are shown in Table 2.
[0054] The specific test results are as follows: Table 2 Results of Anti-fatigue Activity Test
[0055] As shown in Table 2, the mice's weight-bearing swimming time increased by approximately 24.3% after nasal instillation of the fragrance compared to the control group, indicating that the fragrance has a certain anti-fatigue effect on the mice. The liver and kidney weights of the mice were similar to those of the control group, suggesting that nasal instillation of the fragrance does not cause liver and kidney enlargement in mice.
[0056] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A liquid functional fragrance for combating fatigue and boosting immunity, characterized in that: The raw materials, by weight, include 10-30 parts of compound essential oil nanocapsules, 30-50 parts of base oil, 0.1-0.5 parts of preservative, and 20-40 parts of solvent; The composite essential oil nanocapsules use composite essential oil as the core material and modified soy protein isolate and modified chitosan as the wall material. The compound essential oil comprises, by weight percentage, 10-14% angelica essential oil, 8-12% peppermint essential oil, 12-16% rosemary essential oil, 8-12% lemon essential oil, 4-8% eucalyptus oil, 7-11% camellia essential oil, 10-14% nutmeg essential oil, 10-14% rhodiola rosea essential oil, 8-12% ginseng essential oil, 2.5-3.5% vitamin E oil, and 1.5-6.5% neroli essential oil.
2. The anti-fatigue and immunity-enhancing liquid functional fragrance according to claim 1, characterized in that: The compound essential oils comprise, by weight percentage, 14% angelica essential oil, 10% peppermint essential oil, 12% rosemary essential oil, 6% lemon essential oil, 8% eucalyptus oil, 9% camellia essential oil, 14% nutmeg essential oil, 10% rhodiola essential oil, 10% ginseng essential oil, 3% vitamin E oil, and 4% neroli essential oil.
3. The anti-fatigue and immunity-enhancing liquid functional fragrance according to claim 1, characterized in that: The base oils include sweet almond oil, jojoba oil, and grapeseed oil; the mass ratio of the sweet almond oil, jojoba oil, and grapeseed oil is (1-2):(2-3):(4-6).
4. The anti-fatigue and immunity-enhancing liquid functional fragrance according to claim 1, characterized in that: The solvent is a 30-70% (w / w) aqueous solution of propylene glycol.
5. The anti-fatigue and immunity-enhancing liquid functional fragrance according to claim 1, characterized in that: The method for preparing the modified soy protein isolate includes the following steps: Add soy protein isolate to water and mix evenly to obtain a 4-5 mg / mL soy protein isolate solution; adjust the pH of the solution to 11-12 and immediately sonicate; then stir at a constant temperature of 10-20℃ for 30-40 min; adjust the pH to neutral and magnetically stir in an ice-water bath for 15-20 min to obtain modified soy protein isolate.
6. The anti-fatigue and immunity-enhancing liquid functional fragrance according to claim 5, characterized in that: The ultrasonic treatment is performed at a frequency of 20-22 kHz for 5-7 minutes.
7. The anti-fatigue and immunity-enhancing liquid functional fragrance according to claim 1, characterized in that: The modified chitosan is prepared from the following raw materials in parts by weight: 1-2 parts chitosan, 40-80 parts acetic acid solution, 0.05-0.1 parts ascorbic acid, 2-4 parts hydrogen peroxide solution, 0.1-0.3 parts protocatechuic acid, and 20-40 parts sodium acetate buffer solution.
8. The anti-fatigue and immunity-enhancing liquid functional fragrance according to claim 7, characterized in that: The method for preparing the modified chitosan includes the following steps: Dissolve 1-2 parts by weight of chitosan in 40-80 parts by weight of acetic acid solution overnight, and adjust the pH to 5-6. Then, under a nitrogen atmosphere, add a mixture of 0.05-0.1 parts by weight of ascorbic acid and 2-4 parts by weight of hydrogen peroxide solution to the solution and incubate at 40-50°C for 1-2 hours. Subsequently, add 0.1-0.3 parts by weight of protocatechuic acid and incubate at 40-50°C under a nitrogen atmosphere for 24-30 hours. Dialyze the resulting mixture using a dialysis bag with 20-40 parts by weight of sodium acetate buffer at 4-6°C for 12-15 hours, changing the dialysis solution every 4-6 hours. After dialysis, freeze-dry to obtain modified chitosan.
9. A method for preparing an anti-fatigue and immune-enhancing liquid functional fragrance according to any one of claims 1-8, characterized in that: Includes the following steps: S1. Preparation of composite essential oil nanocapsules; S2. Mix the prepared composite essential oil nanocapsules with the base oil and magnetically stir at 300-500 rpm for 30-50 min to fully disperse the microcapsules in the base oil; mix the resulting mixture with propylene glycol aqueous solution and continue stirring for 20-30 min; finally, add the preservative and stir for 10-20 min to obtain the anti-fatigue and immune-enhancing liquid functional fragrance.
10. The method for preparing a liquid functional fragrance for anti-fatigue and immunity enhancement according to claim 9, characterized in that: The preparation method of the composite essential oil nanocapsules includes the following steps: By weight, 13-26 parts of compound essential oil were added to 20-40 parts of modified soy protein isolate solution, followed by 32-64 parts of modified chitosan to obtain a mixed solution. The mixed solution was homogenized at 10000-12000 rpm for 3-4 min, and the pH of the mixed solution was adjusted to 6-7. Then, it was stirred at 50-60℃ and 300-400 rpm for 30-50 min to induce compound coagulation. The solution was then removed and rapidly cooled to 10-15℃ with ice water for 30-40 min to end the reaction. The pH of the system was adjusted to 5-6, and 20-40 parts of transglutaminase were added. The mixture was magnetically stirred and solidified at 10-15℃ for 3-5 h, followed by centrifugation to obtain wet capsules. The wet capsules were pre-cooled for 24-30 h and then freeze-dried for 48-60 h to obtain compound essential oil nanocapsules.