Multi-channel mixed flow injection type perfusion microscope

Abstract

The invention relates to a multi-channel mixed flow injection type perfusion microscope, and relates to the technical field of biological equipment, the multi-channel mixed flow injection type perfusion microscope comprises a flow mixer, the flow mixer comprises a first flow channel module and N second flow channel modules, the number of the second flow channel modules is N, and N is an integer greater than or equal to 1; the first flow channel module and the second flow channel modules are detachably connected, the adjacent second flow channel modules are detachably connected, the injection device further comprises an input assembly and a flow mixing assembly, and the input assembly is provided with a plurality of injection channels capable of being independently opened and closed. Due to the length difference between a first connecting rod and a second connecting rod, a first shifting plate generates asymmetric rotation, so that a solution outside the first flow guide pipe can be slightly stirred, the first flow guide pipe can be promoted to deflect in angle through an irregular vibration rubber plate, dynamic disturbance is formed, local solution retention is avoided in a follow-up flow mixing mode, and different stock solutions are continuously mixed in flowing.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological equipment, and in particular to a multi-channel mixed flow injection type perfusion microscope. BACKGROUND

[0002] In the field of biomedical research, as a core device for observing the physiological state changes of cells in a dynamic solution environment, the perfusion microscope mainly includes a micro-perfusion cavity on the object table, a low-temperature circulating bath for controlling the solution in the micro-perfusion cavity to be in a low-temperature state, an injection syringe for injecting solution into the micro-perfusion cavity, and a suction syringe for extracting solution from the micro-perfusion cavity. Through the cooperation of the injection syringe and the suction syringe, the dynamic balance of the solution in the micro-perfusion cavity can be maintained, thereby ensuring the balance of the solution pressure in the micro-perfusion cavity. In addition, the perfusion microscope also includes a set of devices for observing the changes of cells in the micro-perfusion cavity. The changes of cells in the micro-perfusion cavity are observed after being magnified by an objective lens, and data recording can be performed.

[0003] Most of the existing perfusion microscopes only have one injection syringe and one suction syringe, and it is difficult to measure the volume response of cells under multiple different concentrations of solution. When it is necessary to study the volume response or physiological changes of cells under multiple different concentrations and different components of solution, the solution in the injection syringe needs to be frequently replaced. This not only is cumbersome and time-consuming, but also causes the solution environment in the perfusion cavity to be interrupted or fluctuated, which destroys the continuity of the experiment, easily causes the cells to have a stress response, and affects the accuracy and repeatability of experimental data. Although the improved perfusion microscope (such as the multi-channel mixed flow injection type perfusion microscope disclosed in the patent with the application number 202010036138.2) can achieve the mixing of the original solution by changing the flow direction of the fluid, it still uses an integrated mixed flow device design. The chamber volume of the perfusion cavity and the flow path are fixed structures, and it is difficult to adjust the length and path of the perfusion cavity according to the experimental needs. In addition, the mixing of the mixed flow device mainly depends on the natural convection of the fluid or the fixed direction of the flow structure, and the mixing efficiency is low. Therefore, a multi-channel mixed flow injection type perfusion microscope is proposed. SUMMARY

[0004] In order to overcome the problems in the related art, the present application provides a multi-channel mixed flow injection type perfusion microscope. The mixed flow device is composed of a plurality of flow channel modules which are detachably connected. By increasing or decreasing the number of second flow channel modules, the length of the perfusion cavity can be flexibly adjusted. In addition, by means of the second flow channel modules of different connection surfaces, a perfusion cavity with multiple paths can be assembled.

[0005] In order to achieve the above object, the application provides a multi-channel mixed flow injection type perfusion microscope, comprising a mixed flow device, the mixed flow device comprises a first flow channel module and a second flow channel module, the second flow channel module is provided with N, N is an integer greater than or equal to 1, the first flow channel module and the second flow channel module are combined to form a perfusion cavity, and the first flow channel module and the second flow channel module and the adjacent second flow channel modules are detachably connected, and the perfusion microscope further comprises: an input component, which is detachably arranged in the first flow channel module, and the input component has a plurality of injection channels which can be independently opened and closed; a mixed flow component, which is arranged at least at the connecting part of the adjacent two flow channel modules along the fluid flow direction of the perfusion cavity, and the mixed flow component comprises a flow guide and a driving piece, the flow guide is connected with the adjacent two flow channel modules, and the driving piece is connected with the flow guide to drive the driving piece to rotate when the fluid passes through the flow guide, so that the flow guide is deflected at an angle.

[0006] Preferably, the flow guide comprises a first flow guide pipe, the first flow channel module and the second flow channel module are both provided with a through port, a rubber plate is arranged in the through port, and the first flow guide pipe penetrates through the rubber plate.

[0007] Preferably, the driving piece comprises: a first rotating blade, which is rotatably arranged in the first flow guide pipe; a first connecting rod and a second connecting rod, which are respectively connected with the two ends of the rotating shaft of the first rotating blade, and the length of the first connecting rod is less than that of the second connecting rod, and the end portions of the first connecting rod and the second connecting rod are both provided with a first driving plate.

[0008] Preferably, the mixed flow component further comprises a butt joint component, the butt joint component comprises a first magnetic ring and a second magnetic ring which are magnetically attracted to each other, the first magnetic ring and the second magnetic ring are respectively arranged on the two flow channel modules to be connected, and a sealing ring is further arranged on the connecting end face of the flow channel module.

[0009] Preferably, the butt joint component further comprises a protruding block and a plug rod, the protruding block and the plug rod are respectively arranged on the two flow channel modules to be connected, and a plug groove which is matched with the plug rod is arranged on the protruding block.

[0010] Preferably, the input component comprises: a ring-shaped seat, a plurality of injection channels are arranged on the ring-shaped seat at intervals; a resilient pad, which is arranged in the injection channel, the resilient pad in each injection channel is distributed into two layers in the axial direction, each layer of the resilient pad is composed of two pieces of resilient blocks which can be sealed by abutting, and the abutting and sealing directions of the upper and lower layers of the resilient pad are perpendicular to each other.

[0011] Preferably, the input component further comprises: An input tube is pluggably disposed within the injection channel; A support ring, fixed to the bottom inside the injection channel, is used to support the infusion tube.

[0012] Preferably, the flow guide includes a second flow guide tube, which passes through two adjacent flow channel modules; The actuating element includes: The second rotating blade is rotatably connected inside the second guide tube, and the shaft end of the second rotating blade is connected to a third connecting rod, one end of which extends to the outside of the flow channel module; The first gear and the movable connector are connected to the outer end of the third connecting rod through the movable connector. The outer end of the first gear is connected to a push-pull rod, and the first gear and the third connecting rod can be disconnected or connected through the movable connector. The fourth connecting rod is arranged parallel to the third connecting rod. A second actuating plate is installed on the outside of the fourth connecting rod, and a second gear is installed on the outer end of the fourth connecting rod. The second gear is meshed with the first gear, and the transmission ratio of the first gear and the second gear is 1:3-10.

[0013] Preferably, the movable connector includes: A protruding strip is fixed to the outer wall of the third connecting rod at one end near the first gear. A first connecting cylinder and a second connecting cylinder are rotatably connected, and the end of the second connecting cylinder away from the first connecting cylinder is fixed to a first gear. The interior of both the first and second connecting cylinders is provided with guide grooves for mating protrusions.

[0014] Preferably, it also includes an injection assembly, which includes a barrel and a small pump body. The barrel and the small pump body are connected by an outlet pipe. The outlet end of the small pump body is connected to a connecting pipe that can be connected to an input pipe. A control component is installed on the connecting pipe.

[0015] The technical solution provided by this invention may include the following beneficial effects: 1. In this invention, when the stock solution passes through the first guide tube, it drives the first rotating blade to rotate. Due to the difference in length between the first connecting rod and the second connecting rod, the first actuating plate generates asymmetrical rotation, which not only slightly stirs the solution outside the first guide tube, but also causes the angle of the first guide tube to deflect through the irregular vibration of the rubber plate, forming dynamic disturbance. The follow-up mixing method can avoid local solution stagnation, allowing different stock solutions to be continuously mixed in the flow. Moreover, the solution is stirred twice when it flows through each module connection, realizing segmented mixing and gradual homogenization, providing a stable and uniform solution environment for subsequent cell observation.

[0016] 2. Flexible control of stirring operation: The first gear and the third connecting rod can be disconnected or connected through the movable connector. When multiple types of biological raw materials are added and stirring is required, the rotation of the second rotating blade can drive the second agitator plate to stir the solution, achieving mixing of multiple raw materials. When only one type of biological raw material is added and stirring is not required, the push rod can be pulled outward to disengage the protrusion from the guide groove in the second connecting cylinder, stopping the stirring and mixing operation. This avoids excessive disturbance of the single solution and prevents unnecessary mixing operations from affecting the purity of the raw material. The operation is flexible and convenient.

[0017] 3. The transmission configuration of the first gear and the second gear allows the second gear to rotate more times when the first gear rotates once, thereby improving the stirring efficiency of the second agitator and making the various raw liquids mix more evenly.

[0018] 4. In this invention, the mixer is composed of multiple flow channel modules that are detachably connected. On the one hand, by increasing or decreasing the number of second flow channel modules, the length of the irrigation chamber can be flexibly adjusted, eliminating the need to purchase multiple sets of equipment for different needs. On the other hand, with the help of second flow channel modules with different connection surfaces, irrigation chambers with various paths such as straight, L-shaped, and Z-shaped can be assembled to adapt to different experimental space layouts and process cycles, greatly expanding the applicable scenarios.

[0019] 5. In this invention, staff can input multiple stock solutions through different channels, adjust the solution delivery volume of each channel with the help of control components, and flexibly adjust the stock solution ratio without frequently changing syringes. This effectively solves the problem that traditional single-channel perfusion microscopes cannot continuously measure the volume response of cells under different concentration solutions.

[0020] 6. In this invention, the diversion function can be expanded through the third flow channel module. Its internal diversion components adopt a structure of main pipe-diversion branch pipe-main pipe-more diversion branch pipes, which further improves uniformity. At the same time, multiple sets of second diversion branch pipes can output multiple identical mixed solutions, supporting multiple sets of parallel comparison experiments, reducing experimental errors caused by solution differences, and improving reliability.

[0021] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and are not intended to limit the invention. Attached Figure Description

[0022] The above and other objects, features and advantages of the present invention will become more apparent from the more detailed description of exemplary embodiments of the invention in conjunction with the accompanying drawings, wherein the same reference numerals generally represent the same parts in the exemplary embodiments of the invention.

[0023] Figure 1 This is a schematic diagram of the mixer structure of the present invention; Figure 2This is a structural schematic diagram of the connection state of the two flow channel modules under the first embodiment of the flow guide and actuating component of the present invention; Figure 3 This is a structural schematic diagram of the two flow channel modules in the separated state under the first embodiment of the flow guide and actuating component of the present invention; Figure 4 This is a structural schematic diagram of the first embodiment of the toggle mechanism of the present invention; Figure 5 This is a structural schematic diagram of the connection state of the two flow channel modules under the second embodiment of the flow guide and actuating component of the present invention; Figure 6 This is a structural schematic diagram of the second embodiment of the toggle mechanism of the present invention; Figure 7 This is a schematic diagram of the structure of the present invention in its disassembled state; Figure 8 This is a schematic diagram of the structure of the input component of the present invention; Figure 9 This is a cross-sectional structural schematic diagram of the input component of the present invention; Figure 10 This is a schematic diagram of the structure of the flow divider and the third flow channel module of the present invention; Figure 11 This is a schematic diagram of the structure of the injection component of the present invention.

[0024] The correspondence between the labels and component names in the attached figures is as follows: 1. Mixer; 101. First flow channel module; 102. Second flow channel module; 103. Output port; 2. Input component; 21. Annular seat; 22. Injection channel; 23. Input tube; 24. Elastic pad; 25. Support ring; 3. Mixing assembly; 31. Flow guide; 311. First flow guide pipe; 312. Through port; 313. Rubber plate; 314. Second flow guide pipe; 32. Actuating component; 321. First rotating blade; 322. First connecting rod; 323. Second connecting rod; 324. First actuating plate; 325. Second rotating blade; 326. Third connecting rod; 327. First gear; 328. Push-pull rod; 329. Second gear; 3291. Fourth connecting rod; 3292. Second actuating plate; 33. Connecting assembly; 331. First magnetic ring; 332. Second magnetic ring; 333. Protrusion; 334. Insert rod; 335. Sealing ring; 4. Diversion component; 41. First main pipe; 42. First branch pipe; 43. Second main pipe; 44. Second branch pipe; 5. Third flow channel module; 6. Injection assembly; 61. Bottle body; 62. Miniature pump body; 63. Connecting pipe; 64. Control components; 7. Movable connector; 71. Protruding strip; 72. First connecting cylinder; 73. Second connecting cylinder; 74. Guide groove. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this invention. Preferred embodiments of the invention will now be described in more detail with reference to the accompanying drawings. Although preferred embodiments of the invention are shown in the drawings, it should be understood that the invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to make the invention more thorough and complete, and to fully convey the scope of the invention to those skilled in the art.

[0026] The technical solutions of the embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0027] Example 1: See Figures 1-7As shown, this invention proposes a multi-channel mixed-flow injection perfusion microscope, including a mixer 1. The mixer 1 includes a first flow channel module 101 and a second flow channel module 102. There are N second flow channel modules 102, where N is an integer ≥ 1. The first flow channel module 101 and several second flow channel modules 102 are combined to form an perfusion chamber. Both the first flow channel module 101 and several second flow channel modules 102 have flow chambers. The first flow channel module 101 and the second flow channel modules 102, as well as adjacent second flow channel modules 102, are detachably connected to allow for the assembly of the required number of second flow channel modules 102 to form the desired length of the perfusion chamber. Furthermore, it also includes an input component 2 and a mixing component 3. The input component 2 is detachably disposed in the first flow channel module 101 and has multiple independently openable and closable injection channels 22 through which injection is directed into the first flow channel. The original solution is input into module 101. Along the fluid flow direction of the irrigation chamber, at least one mixing component 3 is set at the connection between two adjacent flow channel modules. That is, a mixing component 3 is set between the first flow channel module 101 and the second flow channel module 102, and between two adjacent second flow channel modules 102. The mixing component 3 includes a guide 31 and a deflector 32. The guide 31 can be deflected to connect two adjacent flow channel modules. The deflector 32 is connected to the guide 31 so that when the fluid passes through the guide 31, the deflector 32 is driven to rotate, thereby driving the guide 31 to achieve angular deflection, so as to achieve dynamic mixing of different fluid original solutions. One end of the irrigation chamber is the fluid input end, specifically the input component 2 connected to the first flow channel module 101, which can be connected to various different types of fluid reagents. The other end is the output port 103, which can also be detachably connected to the second flow channel module 102.

[0028] In addition, multiple sets of mixers 1 can be set up for experiments to facilitate biological experiments.

[0029] By setting up second flow channel modules 102 that can be connected to different surfaces, the second flow channel modules 102 can be used to assemble irrigation cavities of various shapes, including but not limited to straight, L-shaped and Z-shaped, according to the path requirements of the irrigation cavity, so as to meet the needs of different experimental spaces and different cycles.

[0030] Among them, see Figure 3As shown, the mixing assembly 3 also includes a docking assembly 33, which includes a first magnetic ring 331 and a second magnetic ring 332. The first magnetic ring 331 and the second magnetic ring 332 are magnetically attracted to each other and can be stably attracted together. The first magnetic ring 331 and the second magnetic ring 332 are respectively installed on the two flow channel modules to be connected, and a sealing ring 335 is also provided on the connection end face of the flow channel module. Furthermore, the docking assembly 33 also includes a protrusion 333 and a plug 334. The protrusion 333 and the plug 334 are respectively installed on the two flow channel modules to be connected, and a sealing ring 335 is provided on the protrusion 333. A slot is provided to fit the insertion rod 334. The outer end of the insertion rod 334 protrudes outward from the second magnetic ring 332. When assembling the two flow channel modules (taking two second flow channel modules 102 as an example), the latter second flow channel module 102 moves closer to the former second flow channel module 102. First, the insertion rod 334 is aligned with the slot on the protrusion 333 and inserted. After the first magnetic ring 331 and the second magnetic ring 332 are attracted together, it means that the two are connected in place. After the first magnetic ring 331 and the second magnetic ring 332 are magnetically attracted, the two sealing rings 335 are pressed together to achieve a leak-free seal.

[0031] With the docking component 33 in place, no complicated tools are needed. Staff can assemble and disassemble the module manually, which greatly reduces the operating threshold and saves preparation time for biological experiments. Secondly, the detachable design allows staff to quickly assemble or replace the second flow channel module 102 according to the needs of different experimental schemes.

[0032] Secondly, by increasing or decreasing the number of the second flow channel module 102, the overall length of the perfusion chamber can be flexibly changed. For example, a short chamber can be used for rapid reaction experiments (such as instantaneous drug stimulation of cells), while a long chamber can be used for long-term culture experiments (such as continuous cell perfusion observation), without the need to purchase multiple sets of equipment separately for different needs.

[0033] See Figure 1 as well as Figure 8 and Figure 9As shown, the input component 2 includes an annular seat 21, an elastic pad 24, and an input tube 23. The annular seat 21 is detachably installed on the first flow channel module 101, specifically by means of a threaded connection. Multiple injection channels 22 are spaced apart on the annular seat 21. The elastic pad 24 is disposed in the injection channel 22, and the elastic pad 24 in each injection channel 22 is distributed in two layers along the axial direction. Each layer of elastic pad 24 is composed of two elastic blocks that can fit together and seal. The fitting and sealing directions of the upper and lower layers of elastic pad 24 are perpendicular to each other. The input tube 23 is detachably disposed in the injection channel 22. After the input tube 23 is pulled out, the upper and lower layers can close the injection channel 22. When the input tube 23 is inserted, the input tube 23 is squeezed to squeeze the elastic blocks, so that the input tube 23 passes through the two opposing elastic blocks. The elastic blocks are attached to the outer wall of the input tube 23 to ensure sealing. A support ring 25 for supporting the input tube 23 is fixed at the bottom inside the injection channel 22.

[0034] See Figure 11 As shown, the mixed-flow injection perfusion microscope also includes an injection assembly 6. Each input tube 23 corresponds to one injection assembly 6, which can realize the quantitative input of fluid raw materials into each input tube 23. Each injection assembly 6 includes a cylinder 61 and a small pump 62. A detachable cap is installed on the upper end of the cylinder 61. The cylinder 61 is used to hold the stock solution. The cylinder 61 and the small pump 62 are connected by an outlet pipe. The outlet end of the small pump 62 is connected to a connecting pipe 63 that can be connected to the input tube 23. A control component 64 is installed on the connecting pipe 63. The control component 64 includes an electric valve and a flow meter. The flow meter monitors the input flow rate, and the electric valve controls the input amount to realize the input of different stock solutions into different injection channels 22 as needed, so as to achieve different ratio adjustments. By starting the small pump 62, the small pump 62 draws the stock solution and sends it into the input tube 23 through the connecting pipe 63 to realize the input of the stock solution. Alternatively, an existing integrated injection pump can be used to replace the above-mentioned injection assembly 6.

[0035] Among them, see Figure 2 and Figure 3As shown, the flow guide 31 includes a first flow guide tube 311, which is used to guide the raw liquid to the next second flow channel module 102, ensuring the continuity of the raw liquid flow between different modules. The first flow guide tube 311 is provided with a port 312 on both the first flow channel module 101 and the second flow channel module 102. A rubber plate 313 is provided in the port 312. The first flow guide tube 311 passes through the rubber plate 313. The rubber plate 313 has good elastic deformation ability and can tightly wrap the first flow guide tube 311 that passes through it, thereby effectively preventing leakage of the raw liquid during transportation. At the same time, in order to ensure that the rubber plate 313 can maintain reliable recovery force after long-term use and repeated deformation, a spring can be provided inside the rubber plate 313. The elastic potential energy of the spring assists the rubber plate 313 to reset, extend its service life and ensure the sealing effect.

[0036] See Figure 2 and Figure 4 As shown, the actuating component 32 includes a first rotating blade 321 rotatably disposed within the first guide tube 311. One end of the shaft of the first rotating blade 321 is connected to a first connecting rod 322, and the other end of the shaft of the first rotating blade 321 is connected to a second connecting rod 323. The length of the first connecting rod 322 is less than that of the second connecting rod 323. A first actuating plate 324 is installed at the ends of both the first connecting rod 322 and the second connecting rod 323. The first actuating plate 324 is inclined at 10-30°. When two guide components 31 are disposed between two flow channel modules, two actuating components 32 are disposed accordingly. In order to further improve the stirring and mixing effect, the first actuating plates 324 on the two actuating components 32 are preferably disposed in opposite directions. This allows the two first actuating plates 324 to form water flow disturbances in opposite directions when rotating, so that the original liquid forms a more complex flow field in the flow channel module, enhances the fusion between the original liquids, and thus improves the overall mixing efficiency and uniformity.

[0037] Mixing components 3 are set at the connection points of adjacent flow channel modules, so that the solution is agitated twice when it flows through each module connection point, achieving segmented mixing and gradual uniformity. Compared with the existing technology, this dynamic disturbance mixing mechanism significantly improves the mixing uniformity, and the follow-up mixing method can avoid local solution stagnation, allowing different original liquids to be continuously mixed in the flow.

[0038] As described above, after assembling the required mixer 1, the stock solution is input into the first flow channel module 101 from different input pipes 23 through different injection components 6 or injection pumps (the solution concentration is controlled by controlling the delivery volume of each injection component 6). Various stock solutions flow through the perfusion chamber, so that the stock solutions are gradually mixed during the flow process.

[0039] In this process, when the stock solution enters from one flow channel module to another, the solution passes through the first guide tube 311, which drives the first rotating blade 321 to rotate. This, in turn, drives the first connecting rod 322 and the second connecting rod 323 to rotate, thereby causing the first agitator plate 324 to rotate. The first agitator plate 324 can gently stir the solution. The tilted first agitator plate 324 can effectively stir the stock solution outside the first guide tube 311, which helps to promote uniform mixing of the stock solution, avoid local stock solution stagnation, and improve the accuracy of subsequent observation. Secondly, because the first connecting rod 322 and the second connecting rod 323 have different lengths, the rotation of the first rotating blade 321 drives the first agitator plate 324 to move, which will cause the rubber plate 313 to move irregularly. The vibration or deformation of the fluid, due to the difference in length of the connecting rods on both sides, causes the rhythm and amplitude of the action of the first actuating plate 324 on the rubber plate 313 to be inconsistent. This results in the rubber plate 313 not making uniform and regular movements, but exhibiting dynamic changes caused by uneven force. Consequently, the first guide tube 311 can also be agitated within a certain range. Specifically, the asymmetric design changes the stability of the existing symmetrical agitation through structural differentiation. By using connecting rods of different lengths, the fluid generates a velocity difference due to uneven force during movement. This velocity difference will tear apart the originally stable circulation, causing turbulent fluid movement, providing a power basis for the generation of vortices, which can generate more vortices and achieve multi-stage reinforcement of vortices, thereby improving the overall mixing effect.

[0040] Example 2: See Figures 5-7 As shown, the difference between this embodiment and embodiment one is that the flow guide 31 includes a second flow guide pipe 314, which penetrates two adjacent flow channel modules; The actuating element 32 includes a second rotating blade 325, a first gear 327, a movable connector 7, and a fourth connecting rod 3291. The second rotating blade 325 is rotatably connected inside the second guide tube 314, and a third connecting rod 326 is connected to the shaft end of the second rotating blade 325. One end of the third connecting rod 326 extends to the outside of the flow channel module. The first gear 327 is connected to the outer end of the third connecting rod 326 through the movable connector 7. A push-pull rod 328 is connected to the outer end of the first gear 327, and the first gear 327 and the third connecting rod 326 can be disconnected or connected through the movable connector 7. Next, the fourth connecting rod 3291 is set parallel to the third connecting rod 326. A second actuating plate 3292 is installed on the outside of the fourth connecting rod 3291, and a second gear 329 is installed on the outer end of the fourth connecting rod 3291. The second gear 329 is meshed with the first gear 327, and the transmission ratio of the first gear 327 and the second gear 329 is 1:3-10. A protective cover is provided on the outside of the first gear 327 and the second gear 329. A push-pull rod 328 passes through the protective cover. A bearing is provided at the position where the push-pull rod 328 passes through the protective cover, and the push-pull rod 328 is slidably connected to the inner ring of the bearing.

[0041] Among them, see Figure 6 and Figure 7 As shown, the movable connector 7 includes a protrusion 71, a first connecting cylinder 72, and a second connecting cylinder 73. The protrusion 71 is fixed to the outer wall of the third connecting rod 326 near the end of the first gear 327, and multiple protrusions 71 can be arranged in a ring array. The first connecting cylinder 72 and the second connecting cylinder 73 are rotatably connected. Specifically, the first connecting cylinder 72 and the second connecting cylinder 73 can be connected by bearings. When it is necessary to ensure that the third connecting rod 326 does not obstruct the entry of the second connecting cylinder 73, or when an annular groove with a smaller outer diameter and a larger inner diameter is provided at the end of the first connecting cylinder 72, an annular sliding strip is correspondingly provided at the end of the first connecting cylinder 72, and the end of the second connecting cylinder 73 away from the first connecting cylinder 72 is fixed to the first gear 327. The interior of both the first connecting cylinder 72 and the second connecting cylinder 73 is provided with guide grooves 74 that cooperate with the protrusion 71.

[0042] Based on the above, there are two ways to use it in practice: 1. When multiple types of biological stock solutions are added and stirring is required, the protrusion 71 is embedded in the guide groove 74 inside the second connecting cylinder 73. When the biological stock solution enters from one flow channel module to another, the solution can drive the second rotating blade 325 to rotate after passing through the second guide pipe 314, which in turn drives the third connecting rod 326 to rotate. This, in turn, drives the second gear 329 to rotate through the first gear 327, so that the second actuating plate 3292 outside the fourth connecting rod 3291 stirs the solution. Due to the setting of the transmission ratio of the first gear 327 driving the second gear 329, the second gear 329 can rotate more times when the first gear 327 rotates once, thereby improving the uniformity of mixing multiple stock solutions.

[0043] 2. When the added biological stock solution is of a single type and does not require stirring, the operator can pull the push rod 328 to move the first gear 327 outward, thereby pulling the first connecting cylinder 72 and the second connecting cylinder 73 outward a certain distance, so that the protrusion 71 disengages from its embedded state in the guide groove 74 inside the second connecting cylinder 73. At this time, the rotation of the second rotating blade 325 can drive the third connecting rod 326 and the first connecting cylinder 72 to rotate, but will not drive the first gear 327 to rotate through the second connecting cylinder 73. The stirring and mixing operation is stopped without blocking the second guide tube 314, so as to avoid excessive disturbance of the single solution and prevent unnecessary mixing operations from affecting the purity of the stock solution.

[0044] To facilitate determining the outward extension length of the push-pull rod 328, it can be set such that after the push-pull rod 328 is extended to the desired position, the first gear 327 just fits against the outer wall of the protective cover; or a scale line can be set on the push-pull rod 328 for judgment.

[0045] Secondly, to ensure that the convex strip 71 changes from the state of being disengaged from the guide groove 74 inside the second connecting cylinder 73 to the state of being embedded in the guide groove 74 inside the second connecting cylinder 73, a number of guide grooves 74 and convex strips 71 can be arranged in a ring array to facilitate the faster insertion of the convex strip 71 into the guide groove 74 inside the second connecting cylinder 73.

[0046] In addition, the second gear 329 can be installed at the end of the fourth connecting rod 3291 in a detachable chain connection manner, which is convenient for replacement according to the stirring rate.

[0047] Example 3: See Figure 10 As shown, the difference between this embodiment and Embodiment 1 is that the multi-channel mixed-flow injection perfusion microscope also includes a third flow channel module 5. The third flow channel module 5 can be detachably connected to the first flow channel module 101 or the second flow channel module 102. The third flow channel module 5 replaces the output port 103 and is installed at the output end of the perfusion chamber.

[0048] The third flow channel module 5 is equipped with a flow divider 4, which includes a first main pipe 41. The liquid outlet of the first main pipe 41 is connected to M first flow divider branches 42, where M is an integer ≥ 2. The liquid outlets of the M first flow divider branches 42 are connected to a second main pipe 43, and the liquid outlet of the second main pipe 43 is connected to 2M second flow divider branches 44. By mixing-dividing-mixing-dividing the solution, the mixing uniformity can be improved.

[0049] Taking M=2 as an example: the solution flowing out of the perfusion chamber is sent to two first branch pipes 42 via the first main pipe 41, and then mixed through the second main pipe 43. After mixing, it can flow out from four second branch pipes 44. Parallel comparison experiments can be conducted by observing the solutions in different second branch pipes 44, reducing experimental errors caused by solution differences and improving experimental reliability.

[0050] The present invention has been described in detail above with reference to the accompanying drawings. In the above embodiments, the descriptions of each embodiment have their own emphasis; for parts not described in detail in a certain embodiment, please refer to the relevant descriptions of other embodiments. Those skilled in the art should also understand that the actions and modules involved in the specification are not necessarily essential to the present invention. Furthermore, it is understood that the steps in the method of the embodiments of the present invention can be adjusted, combined, and deleted according to actual needs, and the structure in the device of the embodiments of the present invention can be combined, divided, and deleted according to actual needs.

[0051] The various embodiments of the present invention have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A multi-channel mixed-flow injection-type perfusion microscope, comprising a mixer (1), characterized in that, The mixer (1) includes a first flow channel module (101) and a second flow channel module (102). There are N second flow channel modules (102), where N is an integer ≥ 1. The first flow channel module (101) and several second flow channel modules (102) are combined to form an irrigation chamber. The first flow channel module (101) and the second flow channel module (102), as well as adjacent second flow channel modules (102), are detachably connected. The mixer also includes: The input component (2) is detachably disposed in the first flow channel module (101), and the input component (2) has multiple injection channels (22) that can be opened and closed independently. The mixing component (3) is provided at least one of the mixing components (3) at the connection of two adjacent flow channel modules along the fluid flow direction of the irrigation cavity. The mixing component (3) includes a guide (31) and a deflector (32). The guide (31) connects two adjacent flow channel modules, and the deflector (32) is connected to the guide (31) so that when the fluid passes through the guide (31), the deflector (32) is driven to rotate, thereby driving the guide (31) to achieve angular deflection.

2. The multi-channel mixed-flow injection perfusion microscope according to claim 1, characterized in that, The flow guide (31) includes a first flow guide tube (311), and both the first flow channel module (101) and the second flow channel module (102) are provided with a port (312). A rubber plate (313) is provided inside the port (312), and the first flow guide tube (311) passes through the rubber plate (313).

3. The multi-channel mixed-flow injection perfusion microscope according to claim 2, characterized in that, The actuating element (32) includes: The first rotating blade (321) is rotatably disposed inside the first guide tube (311); The first connecting rod (322) and the second connecting rod (323) are respectively connected to the two ends of the shaft of the first rotating blade (321), and the length of the first connecting rod (322) is less than that of the second connecting rod (323). The ends of the first connecting rod (322) and the second connecting rod (323) are each equipped with a first actuating plate (324).

4. The multi-channel mixed-flow injection perfusion microscope according to claim 1, characterized in that, The mixing component (3) further includes a docking component (33), which includes a first magnetic ring (331) and a second magnetic ring (332) that are magnetically attracted to each other. The first magnetic ring (331) and the second magnetic ring (332) are respectively installed on the two flow channel modules to be connected, and a sealing ring (335) is also provided on the connection end face of the flow channel module.

5. The multi-channel mixed-flow injection perfusion microscope according to claim 4, characterized in that, The docking assembly (33) further includes a protrusion (333) and a plug (334), which are respectively installed on the two flow channel modules to be connected, and the protrusion (333) has a slot adapted to the plug (334).

6. The multi-channel mixed-flow injection perfusion microscope according to claim 1, characterized in that, The input component (2) includes: A ring seat (21), with multiple injection channels (22) spaced apart on the ring seat (21); An elastic pad (24) is disposed in the injection channel (22). The elastic pad (24) in each injection channel (22) is distributed in two layers along the axial direction. Each layer of the elastic pad (24) is composed of two elastic blocks that can fit together and seal. The fitting and sealing directions of the upper and lower elastic pads (24) are perpendicular to each other.

7. The multi-channel mixed-flow injection perfusion microscope according to claim 6, characterized in that, The input component (2) further includes: The input tube (23) is pluggably disposed within the injection channel (22); The support ring (25) is fixed to the bottom inside of the injection channel (22) and is used to support the inlet tube (23).

8. The multi-channel mixed-flow injection perfusion microscope according to claim 1, characterized in that, The flow guide (31) includes a second flow guide tube (314), which passes through two adjacent flow channel modules; The actuating element (32) includes: The second rotating blade (325) is rotatably connected inside the second guide tube (314), and the shaft end of the second rotating blade (325) is connected to a third connecting rod (326), one end of the third connecting rod (326) extending to the outside of the flow channel module; The first gear (327) and the movable connector (7) are connected to the outer end of the third connecting rod (326) through the movable connector (7). The outer end of the first gear (327) is connected to a push-pull rod (328), and the first gear (327) and the third connecting rod (326) can be disconnected or connected through the movable connector (7). The fourth connecting rod (3291) is arranged parallel to the third connecting rod (326). A second actuating plate (3292) is installed on the outside of the fourth connecting rod (3291), and a second gear (329) is installed on the outer end of the fourth connecting rod (3291). The second gear (329) is meshed with the first gear (327), and the transmission ratio of the first gear (327) and the second gear (329) is 1:3-10.

9. The multi-channel mixed-flow injection perfusion microscope according to claim 8, characterized in that, The movable connector (7) includes: A protruding strip (71) is fixed to one end of the outer wall of the third connecting rod (326) near the first gear (327); The first connecting cylinder (72) and the second connecting cylinder (73) are rotatably connected, and the end of the second connecting cylinder (73) away from the first connecting cylinder (72) is fixed to the first gear (327). The first connecting cylinder (72) and the second connecting cylinder (73) are both provided with guide grooves (74) that cooperate with the protrusion (71).

10. The multi-channel mixed-flow injection perfusion microscope according to claim 7, characterized in that, It also includes an injection assembly (6), which includes a barrel (61) and a small pump body (62). The barrel (61) and the small pump body (62) are connected by an outlet pipe. The outlet end of the small pump body (62) is connected to a connecting pipe (63) that can be connected to the input pipe (23). A control component (64) is installed on the connecting pipe (63).

Images