Fermented feed rich in vitamin C and preparation method thereof

Abstract

The invention belongs to the field of animal feed and bioengineering, and particularly relates to a fermented feed rich in vitamin C and a preparation method thereof. The fermented feed rich in vitamin C is prepared from the following preparation raw materials in parts by weight: 30 to 50 parts of corn flour, 15 to 25 parts of bran, 1 to 3 parts of calcium ascorbate, 0.1 to 0.3 part of vitamin D3, 0.1 to 0.3 part of vitamin E, 0.3 to 0.5 part of lactic acid bacteria, 0.2 to 0.4 part of saccharomycetes, 0.05 to 0.15 part of xylooligosaccharide, 1 to 2 parts of calcium hydrophosphate, 0.2 to 0.4 part of trace element premix, 0.4 to 0.5 part of lysine, 0.1 to 0.3 part of methionine, 0.3 to 0.4 part of cellulase and 0.1 to 0.3 part of citric acid. And 0.05 part of plant essential oil. Through the addition of calcium ascorbate and the regulation and control addition of other functional components, the nutrition balance and biological activity of the feed are comprehensively ensured, the immunity of animals is remarkably improved, and the quality of the fermented feed is comprehensively ensured.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of animal feed and bioengineering, and specifically relates to a vitamin C-rich fermented feed and a preparation method thereof. BACKGROUND

[0002] As a high-efficiency and environmentally friendly technology, fermented feed has been widely used in the livestock industry in recent years. Through fermentation process, the nutritional utilization rate of feed can be improved and the immunity of animals can be enhanced, which is an important direction for the green and sustainable development of modern livestock industry. Calcium ascorbate, as an important vitamin C supplement, has higher stability and better water solubility compared to ordinary vitamin C, and is considered a more ideal vitamin C supplement. Calcium ascorbate has significant antioxidant and immune regulation functions, can promote collagen synthesis in the animal body, enhance immunity, and has an important role in the growth and development and health of animals. In particular, calcium ascorbate is more stable than ordinary vitamin C in humid and high-temperature environments and can better withstand the extreme conditions during the fermentation process, thereby retaining its activity and ensuring the sustained release and effectiveness of vitamin C. Adding calcium ascorbate to feed can significantly improve the nutritional value of feed and provide more essential nutrients for animals.

[0003] The prior art mainly adds vitamin C, L-ascorbic acid, etc. The present application adds calcium ascorbate to the fermented feed, optimizes the fermentation process and addition method, and effectively improves the nutritional value and quality of the feed. The present application not only meets the animal's demand for vitamin C, but also has the advantages of simple process, low cost, and wide applicability, providing a new technical approach for the industrial production of fermented feed. SUMMARY

[0004] In view of the deficiencies of the prior art, the present application provides a vitamin C-rich fermented feed. By adding calcium ascorbate and other functional ingredients, the nutritional balance and biological activity of the feed are comprehensively ensured, the immunity of animals is significantly improved, and the quality of the fermented feed is comprehensively ensured.

[0005] In order to achieve the above-mentioned purpose, the present application is implemented by the following technical scheme: a vitamin C-rich fermented feed, comprising the following preparation raw materials:

[0006] corn flour, bran, calcium ascorbate, vitamin D3, vitamin E, lactic acid bacteria, yeast, xylooligosaccharide, calcium hydrogen phosphate, trace element premix, lysine, methionine, cellulase, citric acid, and plant essential oil;

[0007] The specific ratio by weight is as follows:

[0008] Corn flour 30-50 parts, bran 15-25 parts, calcium ascorbate 1-3 parts, vitamin D3 0.1-0.3 parts, vitamin E 0.1-0.3 parts, lactic acid bacteria 0.3-0.5 parts, yeast 0.2-0.4 parts, xylo-oligosaccharide 0.05-0.15 parts, calcium hydrogen phosphate 1-2 parts, trace element premix 0.2-0.4 parts, lysine 0.4-0.5 parts, methionine 0.1-0.3 parts, cellulase 0.3-0.4 parts, citric acid 0.1-0.3 parts, and plant essential oil 0.05 parts.

[0009] Further optimization of the technical solution, by weight parts, includes the following raw materials: corn flour 40 parts, bran 20 parts, calcium ascorbate 1 part, vitamin D3 0.2 parts, vitamin E 0.2 parts, lactic acid bacteria 0.4 parts, yeast 0.3 parts, xylo-oligosaccharide 0.1 parts, calcium hydrogen phosphate 1.5 parts, trace element premix 0.3 parts, lysine 0.45 parts, methionine 0.2 parts, cellulase 0.35 parts, citric acid 0.2 parts, and plant essential oil 0.05 parts.

[0010] Further optimization of the technical solution, by weight parts, includes the following raw materials: corn flour 40 parts, bran 20 parts, calcium ascorbate 1 part, vitamin D3 0.2 parts, vitamin E 0.2 parts, lactic acid bacteria 0.4 parts, yeast 0.3 parts, xylo-oligosaccharide 0.1 parts, calcium hydrogen phosphate 1.5 parts, trace element premix 0.3 parts, lysine 0.45 parts, methionine 0.2 parts, cellulase 0.35 parts, citric acid 0.2 parts, and plant essential oil 0.05 parts.

[0011] Further optimization of the technical solution, by weight parts, includes the following raw materials: corn flour 40 parts, bran 20 parts, calcium ascorbate 1 part, vitamin D3 0.2 parts, vitamin E 0.2 parts, lactic acid bacteria 0.4 parts, yeast 0.3 parts, xylo-oligosaccharide 0.1 parts, calcium hydrogen phosphate 1.5 parts, trace element premix 0.3 parts, lysine 0.45 parts, methionine 0.2 parts, cellulase 0.35 parts, citric acid 0.2 parts, and plant essential oil 0.05 parts.

[0012] A method for preparing a fermented feed rich in vitamin C, comprising the following steps:

[0013] Step one: pretreat corn flour and bran, uniformly mix corn flour, bran, calcium hydrogen phosphate, trace element premix, lysine, methionine and other ingredients according to the proportion, and ensure that the mixture is not caked, then add calcium ascorbate, vitamin D3, vitamin E, xylo-oligosaccharide and citric acid in batches, continue to stir until uniformly mixed, and then slowly add cellulase and plant essential oil after the mixture is cooled and stirred uniformly;

[0014] Step two: activate the lactic acid bacteria and yeast at 30-35 DEG C for 1-2h, mix the activated lactic acid bacteria and yeast according to the proportion to prepare a compound inoculum, and uniformly spray the compound inoculum into the raw materials to ensure that the moisture content of the feed reaches 40%-50%, then put the mixed raw materials into a fermentation container, seal after exhausting air, and ferment at 30-35 DEG C for 48-72 hours, and regularly monitor the pH value and temperature during the fermentation process to ensure the fermentation effect.

[0015] Step three: after the fermentation is completed, filter the fermented feed, and dry the feed using a drying device. After the fermented feed is cooled to room temperature, antioxidant treatment is performed, followed by packaging and storage.

[0016] Compared with the prior art, the above technical scheme has the following beneficial effects:

[0017] The present application not only maintains the original nutritional value, but also optimizes the addition of vitamin C by adding calcium ascorbate, the strain ratio, the timing of enzyme addition and the use of plant essential oils, significantly improving the fermentation effect, stability and animal digestion and absorption capacity of the feed. Finally, a fermented feed rich in vitamin C, easy to digest and high in nutritional value is obtained.

[0018] The present application can better meet the nutritional needs of animals in practical application, improve growth performance and health status, and has a wide market application prospect. DETAILED DESCRIPTION

[0019] The present application will be further described in detail below in conjunction with examples, but the embodiments of the present application are not limited thereto. Unless otherwise specified, the reagents, methods and equipment used in the present application are conventional reagents, methods and equipment in the technical field. Unless otherwise specified, the test methods in the following examples are generally carried out according to conventional experimental conditions or according to the experimental conditions recommended by the manufacturer. Unless otherwise specified, the reagents and raw materials used in the present application are commercially available.

[0020] A method for preparing a fermented feed rich in vitamin C, comprising the following steps:

[0021] Step one: pretreat the corn flour and bran, uniformly mix the corn flour, bran, calcium hydrogen phosphate, trace element premix, lysine and methionine according to the proportion, and ensure that the mixture is free of lumps, then add calcium ascorbate, vitamin D3, vitamin E, xylo-oligosaccharides and citric acid in batches, continue to stir until uniformly mixed, and slowly add cellulase and plant essential oil after the mixture is cooled and stirred uniformly.

[0022] The pretreatment steps of corn flour and bran are as follows:

[0023] 1. Pulverization and screening: Corn meal and bran are separately pulverized to a fineness of 80-100 mesh (about 0.15-0.18 mm) to ensure uniform particles for subsequent mixing and fermentation. Use a vibrating screen or air flow screen to remove stones, metal scraps and other impurities.

[0024] 2. Moisture adjustment and sterilization:

[0025] Control the moisture content of the raw materials at 10-12% (by drying or airing) to avoid mold growth during storage due to excessive moisture.

[0026] 3. Perform mild heat sterilization treatment (such as steam treatment at 100-105°C for 10-15 minutes or microwave treatment) to kill potential pathogenic bacteria and mold spores, but not to destroy nutritional ingredients (avoid loss of vitamin C due to high temperature).

[0027] 4. Preparation before mixing: After pretreatment, corn meal and bran need to be temporarily stored in a dry and clean environment to prevent moisture absorption or secondary contamination.

[0028] Step two: Activate the lactic acid bacteria and yeast at 30-35°C for 1-2 hours, mix the activated lactic acid bacteria and yeast according to the proportion to make a composite inoculum, and evenly spray the composite inoculum onto the raw materials to ensure the moisture content of the feed reaches 40-50%. After spraying, the mixed raw materials are loaded into the fermentation container, and after exhausting the air, it is sealed. The fermentation temperature is 30-35°C, and the fermentation time is 48-72 hours. Regularly monitor the pH value and temperature during the fermentation process to ensure the fermentation effect.

[0029] The change of pH value is as follows:

[0030] 1. Initial stage of fermentation (0-24 hours): The pH may start to decrease from 6.0-7.0, and it needs to be controlled above 5.0 to avoid the outbreak of miscellaneous bacteria.

[0031] 2. Mid-stage of fermentation (24-48 hours): Lactic acid accumulates, and the pH should be reduced to 4.2-4.5, which is the key point of inhibiting bacteria (lower than 4.5 can effectively inhibit harmful bacteria such as Escherichia coli).

[0032] 3. Late stage of fermentation (48-72 hours): The pH is stable at 4.0-4.5, and if it is lower than 4.0, it may lead to over-fermentation (high acidity affects palatability).

[0033] Step three: After the fermentation is completed, the fermented feed is filtered, dried using drying equipment, and then antioxidant treatment is performed when the fermented feed cools to room temperature. Subsequently, it is packaged and stored.

[0034] The standards for completed fermentation are as follows:

[0035] 1. pH stability: pH is stable at 4.0-4.5, indicating that lactic acid fermentation is fully completed.

[0036] 2. Temperature stability: temperature is maintained at 30-35℃ with a fluctuation of <0.5℃, and fermentation heat production stops (no warm feeling on touching the container wall).

[0037] 3. Sensory indicators:

[0038] Odor: has a strong sour smell (similar to yogurt or pickles), no odor (such as corruption, moldy smell).

[0039] Texture: feed is loose and granular, no clumping or slime (indicating contamination by miscellaneous bacteria).

[0040] 4. Microbial activity: lactic acid bacteria and yeast bacteria reach peak levels (>1×10 9 CFU / g), then decrease to a stable level (rapid detection by test paper).

[0041] 5. Time reference: minimum fermentation time is 48 hours, but not more than 72 hours.

[0042] Example 1

[0043] A vitamin C-rich fermented feed (E1) includes the following raw materials for preparation:

[0044] Corn meal, bran, calcium ascorbate, vitamin D3, vitamin E, lactic acid bacteria, yeast, xylo-oligosaccharides, calcium hydrogen phosphate, trace element premix, lysine, methionine, cellulase, citric acid, plant essential oil;

[0045] The specific ratio by weight is as follows:

[0046] Corn meal 40 parts, bran 20 parts, calcium ascorbate 1 part, vitamin D3 0.2 parts, vitamin E 0.2 parts, lactic acid bacteria 0.4 parts, yeast 0.3 parts, xylo-oligosaccharides 0.1 parts, calcium hydrogen phosphate 1.5 parts, trace element premix 0.3 parts, lysine 0.45 parts, methionine 0.2 parts, cellulase 0.35 parts, citric acid 0.2 parts, plant essential oil 0.05 parts.

[0047] Example 2

[0048] A vitamin C-rich fermented feed (E2) includes the following raw materials for preparation:

[0049] Corn meal, bran, calcium ascorbate, vitamin D3, vitamin E, lactic acid bacteria, yeast, xylo-oligosaccharides, calcium hydrogen phosphate, trace element premix, lysine, methionine, cellulase, citric acid, plant essential oil;

[0050] The specific ratio by weight is as follows:

[0051] Corn flour 40 parts, bran 20 parts, calcium ascorbate 2 parts, vitamin D3 0.2 parts, vitamin E 0.2 parts, lactic acid bacteria 0.4 parts, yeast 0.3 parts, xylo-oligosaccharide 0.1 parts, calcium hydrogen phosphate 1.5 parts, trace element premix 0.3 parts, lysine 0.45 parts, methionine 0.2 parts, cellulase 0.35 parts, citric acid 0.2 parts, plant essential oil 0.05 parts.

[0052] Example 3

[0053] A vitamin C-rich fermented feed (E3) includes the following raw materials for preparation:

[0054] Corn flour, bran, calcium ascorbate, vitamin D3, vitamin E, lactic acid bacteria, yeast, xylo-oligosaccharide, calcium hydrogen phosphate, trace element premix, lysine, methionine, cellulase, citric acid, plant essential oil;

[0055] The specific ratio by weight parts is as follows:

[0056] Corn flour 40 parts, bran 20 parts, calcium ascorbate 3 parts, vitamin D3 0.2 parts, vitamin E 0.2 parts, lactic acid bacteria 0.4 parts, yeast 0.3 parts, xylo-oligosaccharide 0.1 parts, calcium hydrogen phosphate 1.5 parts, trace element premix 0.3 parts, lysine 0.45 parts, methionine 0.2 parts, cellulase 0.35 parts, citric acid 0.2 parts, plant essential oil 0.05 parts.

[0057] Trace element premix generally includes the following core ingredients: iron (Fe): supports blood formation and immunity; zinc (Zn): promotes enzyme activity and skin health; copper (Cu): involved in metabolism and bone development; manganese (Mn): critical to bone and reproductive system function; iodine (I): important for thyroid hormone synthesis; selenium (Se): as an antioxidant, protects cells; cobalt (Co): involved in the synthesis of vitamin B12.

[0058] Control group: a fermented feed (E) includes the following raw materials for preparation:

[0059] Corn flour, bran, vitamin D3, vitamin E, lactic acid bacteria, yeast, xylo-oligosaccharide, calcium hydrogen phosphate, trace element premix, lysine, methionine, cellulase, citric acid, plant essential oil;

[0060] The specific ratio by weight parts is as follows:

[0061] Corn meal 40 parts, bran 20 parts, vitamin D3 0.2 parts, vitamin E 0.2 parts, lactic acid bacteria 0.4 parts, yeast 0.3 parts, xylo-oligosaccharide 0.1 parts, calcium hydrogen phosphate 1.5 parts, trace element premix 0.3 parts, lysine 0.45 parts, methionine 0.2 parts, cellulase 0.35 parts, citric acid 0.2 parts, and plant essential oil 0.05 parts.

[0062] In order to verify the influence of the fermented feed prepared by the examples 1-3 and the control example of the present application on the growth performance of animals, the following method is used to explore: the fermented feed rich in vitamin C of the present application is applied in the breeding of broilers, and during the test period, 4 groups are randomly divided, each group has 3 repeats, and each repeat has 10 chickens. The compound feed prepared by the examples 1-3 and the control example is respectively fed to the above-mentioned 4 groups of broilers, and the feeding lasts for 42 days. During the test period, the chickens are free to eat and drink water. At the age of 42 days of the broilers, 3 chickens are randomly selected from each repeat, and the body weight and feed intake of all the broilers are detected and counted, and the average weight, average daily weight gain and average daily feed intake in the early stage (1-21 days) and the whole period (1-42 days) are calculated. At the same time, at the end of the experiment, 3 broilers are randomly selected from each repeat for blood sampling, and the content of vitamin C in the serum is determined by using a biochemical analyzer. The specific determination results are recorded in Table 1.

[0063] At the same time, in order to verify the influence of the fermented feed prepared by the examples 1-3 and the control example of the present application in the actual application of animals, the following method is used to explore: the fermented feed rich in vitamin C of the present application is applied in the breeding of broilers, and during the test period, the weight gain and feed intake of all the broilers are detected and counted, and the number of deaths of various broilers during the test period is recorded. The specific results are recorded in Table 1. The specific determination results are recorded in Table 1.

[0064] Table 1 Influence of fermented feed of each group on growth performance of broilers

[0065] From the experimental results of Table 1, the average daily weight gain, daily feed intake and vitamin C content of the broilers in the example 1-3 groups are higher than those in the control group, and the average daily weight gain, daily feed intake and vitamin C content of the experimental example 3 with higher ascorbic acid calcium addition are higher. Compared with the example 1-3 group, the control group does not add ascorbic acid calcium in the feed additive, and the daily weight gain and feed intake of the broilers in the control group are not as good as those in the example. It can be seen that ascorbic acid calcium plays an important role in improving the daily weight gain, daily feed intake and vitamin C content of animals. On the one hand, the ascorbic acid calcium added in the feed of the application can directly supplement the required vitamin C of animals, enhance immunity, and promote the growth and development of animals. At the same time, the provision of calcium elements in ascorbic acid calcium helps the health of animal bones and teeth, so that animals can maintain normal functions of nerves and muscles. On the other hand, the addition of ascorbic acid calcium in the feed can improve the quality of fermented feed, enhance the flavor of feed, and improve the feed intake and utilization rate of animals.

[0066] Table 2 Influence of fermented feed in each group on actual application of animals

[0067] From the experimental results of Table 2, the feed conversion ratio and dead rate of the broilers in the example 1-3 groups are lower than those in the control group, and the feed conversion ratio and dead rate of the experimental example 3 with higher ascorbic acid calcium addition are lower. It can be seen that the efficient use of vitamin C-rich fermented feed can reduce breeding costs and increase economic benefits for breeding enterprises. The reduction of dead rate also shows that the addition of ascorbic acid calcium effectively improves the immunity and health level of animals, so that the mortality of broilers is reduced. In summary, the application of vitamin C-rich fermented feed can improve the growth performance of animals and significantly improve the economic benefits of farms. Therefore, the vitamin C-rich fermented feed provided by the application has a broad application prospect in actual livestock production, and can help breeders achieve the goal of high efficiency and sustainable feeding.

[0068] It is to be noted that, in the present text, terms such as first and second, and the like, merely serve to identify a difference between one entity or action and another entity or action, and do not necessarily require or imply that there is any such actual relationship or order between these entities or actions. Moreover, the terms "comprising", "including", or any other variant thereof are intended to cover a non-exclusive inclusion, such that processes, methods, articles, or apparatuses that comprise a list of elements are not required to comprise only those elements, but can include other elements not expressly listed or inherent to such processes, methods, articles, or apparatuses. Without further limitation, an element preceded by "comprises a" or "comprises" does not, without more limitations, preclude the existence of further elements of the process, method, article, or apparatus that includes the element. Furthermore, in the present text, "greater than", "less than", "exceed", and the like are understood to exclude the number itself; "and above", "and below", "within", and the like are understood to include the number itself.

[0069] Although the above-mentioned embodiments have been described, those skilled in the art can make further changes and modifications to these embodiments once they know the basic inventive concept, so the above description is only for the embodiments of the present application, and does not limit the patent protection scope of the present application, and any equivalent structure or equivalent process transformation, or direct or indirect application in other related technical fields made by using the content of the present application specification, are also included in the patent protection scope of the present application.

Claims

1. A fermented feed rich in vitamin C, characterized in that, The ingredients include: corn flour, wheat bran, calcium ascorbate, vitamin D3, vitamin E, lactic acid bacteria, yeast, xylooligosaccharides, dicalcium phosphate, trace element premix, lysine, methionine, cellulase, citric acid, and plant essential oils. The specific proportions by weight are as follows: Corn flour 30-50 parts, wheat bran 15-25 parts, calcium ascorbate 1-3 parts, vitamin D3 0.1-0.3 parts, vitamin E 0.1-0.3 parts, lactic acid bacteria 0.3-0.5 parts, yeast 0.2-0.4 parts, xylooligosaccharides 0.05-0.15 parts, dicalcium phosphate 1-2 parts, trace element premix 0.2-0.4 parts, lysine 0.4-0.5 parts, methionine 0.1-0.3 parts, cellulase 0.3-0.4 parts, citric acid 0.1-0.3 parts, and plant essential oil 0.04-0.06 parts.

2. The fermented feed rich in vitamin C as described in claim 1, characterized in that, By weight, the ingredients include: 40 parts corn flour, 20 parts wheat bran, 1 part calcium ascorbate, 0.2 parts vitamin D3, 0.2 parts vitamin E, 0.4 parts lactic acid bacteria, 0.3 parts yeast, 0.1 parts xylooligosaccharides, 1.5 parts dicalcium phosphate, 0.3 parts trace element premix, 0.45 parts lysine, 0.2 parts methionine, 0.35 parts cellulase, 0.2 parts citric acid, and 0.05 parts plant essential oil.

3. The fermented feed rich in vitamin C as described in claim 1, characterized in that, By weight, the ingredients include: 40 parts corn flour, 20 parts wheat bran, 2 parts calcium ascorbate, 0.2 parts vitamin D3, 0.2 parts vitamin E, 0.4 parts lactic acid bacteria, 0.3 parts yeast, 0.1 parts xylooligosaccharides, 1.5 parts dicalcium phosphate, 0.3 parts trace element premix, 0.45 parts lysine, 0.2 parts methionine, 0.35 parts cellulase, 0.2 parts citric acid, and 0.05 parts plant essential oil.

4. The fermented feed rich in vitamin C as described in claim 1, characterized in that, By weight, the ingredients include: 40 parts corn flour, 20 parts wheat bran, 3 parts calcium ascorbate, 0.2 parts vitamin D3, 0.2 parts vitamin E, 0.4 parts lactic acid bacteria, 0.3 parts yeast, 0.1 parts xylooligosaccharides, 1.5 parts dicalcium phosphate, 0.3 parts trace element premix, 0.45 parts lysine, 0.2 parts methionine, 0.35 parts cellulase, 0.2 parts citric acid, and 0.05 parts plant essential oil.

5. The fermented feed rich in vitamin C as described in claim 1, characterized in that, The trace element premix includes one or more of the following: iron, zinc, copper, manganese, iodine, selenium, and cobalt.

6. A method for preparing fermented feed rich in vitamin C, characterized in that, Includes the following steps: Step 1: Pre-treat the corn flour and bran. Mix the corn flour, bran, dicalcium phosphate, trace element premix, lysine and methionine in a uniform ratio, ensuring that the mixture is free of lumps. Then, add calcium ascorbate, vitamin D3, vitamin E, xylooligosaccharides and citric acid in batches, and continue stirring until uniformly mixed. After the mixture cools, slowly add cellulase and plant essential oils and stir until uniform. Step 2: Activate the lactic acid bacteria and yeast at 30-35℃ for 1-2 hours. Mix the activated lactic acid bacteria and yeast in a certain proportion to make a compound inoculum solution. Spray the compound inoculum solution evenly onto the raw materials, ensuring that the feed moisture reaches 40%-50%. After spraying, put the mixed raw materials into a fermentation container, remove the air, and seal it. The fermentation temperature is 30-35℃, and the fermentation time is 48-72 hours. Regularly monitor the pH value and temperature during the fermentation process to ensure the fermentation effect. Step 3: After fermentation is complete, filter the fermented feed, dry it using a drying device, and then perform an antioxidant treatment after the fermented feed has cooled to room temperature. Finally, package and store it.

7. The method for preparing fermented feed rich in vitamin C as described in claim 6, characterized in that, The preprocessing in step one includes: Step 1.1, Crushing and Screening: Crush the corn flour and bran to a fineness of 80-100 mesh to ensure uniform particle size, which facilitates subsequent mixing and fermentation. Use a vibrating screen or air classifier to remove stones, metal shavings and other impurities. Step 1.2, Moisture Adjustment and Sterilization: Control the moisture content of raw materials to 10%–12%; Step 1.3: Perform a light heat sterilization treatment to kill potential pathogens and mold spores; Step 1.4, Preparation before mixing: After pretreatment, corn flour and bran need to be temporarily stored in a dry and clean environment to prevent moisture absorption or secondary contamination.

8. The method for preparing fermented feed rich in vitamin C as described in claim 6, characterized in that, The mild heat sterilization treatment in step 1.3 is: steam treatment at 100-105°C for 10-15 minutes or microwave treatment.

9. The method for preparing fermented feed rich in vitamin C as described in claim 6, characterized in that, In step three, fermentation is complete when: the pH stabilizes at 4.0–4.5; the temperature is maintained at 30–35°C with fluctuations of <0.5°C; and fermentation heat production ceases.

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