A composition containing peony for whitening and anti-aging and a preparation method thereof

Abstract

The present application relates to a kind of white anti-aging composition containing peony and its preparation method, specifically to utilize the fermentation product of mixed fermentation of peony flower petal extract, ginseng flower petal extract and safflower petal extract by composite microbial inoculant, the composition of the present application can be safe, can efficiently remove free radical, effectively anti-aging, improve skin microcirculation, and have good storage stability.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically, to a whitening and anti-aging composition containing peony and its preparation method. Background Technology

[0002] In recent years, with the continuous development of the cosmetics industry, consumers have become more stringent in their selection of cosmetic ingredients, especially favoring cosmetics made from natural herbal raw materials. Furthermore, research on traditional Chinese medicine cosmetics has become a research hotspot in my country's cosmetics market. Consumer demand for natural cosmetics made from traditional Chinese herbs and extracts is driving the development and utilization of traditional Chinese medicine beauty formulas in cosmetics. Raw material innovation is a core focus in the skincare field. Skincare products on the market can be broadly divided into two categories: traditional skincare products containing chemical ingredients and skincare products containing plant-based ingredients that have gradually emerged in recent years. Peony (Paeonia suffruticosa) belongs to the Ranunculaceae family and the Paeonia genus. It is a perennial deciduous shrub with a wide distribution, mainly growing in the Yangtze and Yellow River basins, such as Henan, Shandong, and Anhui provinces. Peony flowers have a long history of use in my country for food and medicine. Peony flowers contain protein, fat, fatty acids, flavonoids, and trace elements. Besides its ornamental, medicinal, and edible uses, peonies have also been researched and applied in skincare in recent years, mainly focusing on peony root bark, seeds, and flowers. Peony root bark (also known as peony bark or danpi) is a traditional Chinese medicine. Numerous studies have found it to be rich in various active substances such as paeonol, paeonol glycoside, and paeoniflorin, exhibiting excellent antioxidant and anti-aging effects. Peony seed oil, produced from peony seeds, possesses strong antioxidant capabilities and can be used as a base oil or active ingredient in skincare products. Research indicates that gas chromatography-mass spectrometry (GC-MS) analysis of peony flower components detected 78 compounds. Analysis of the antioxidant activity of petal extracts revealed that the DPPH free radical scavenging activity of peony petal extracts was higher than 200 μg / mL BHT, demonstrating significant antioxidant effects. Furthermore, peony bud extract and peony flower water extract both exhibit antioxidant and whitening activities.

[0003] In addition, ginseng is a renowned and precious medicinal herb, with a history of over 4,000 years of use for health preservation and disease treatment. It is also a highly valued beauty product, historically known as the "elixir for wrinkles." Ginseng contains ginsenosides, essential oils, ginseng acid, vitamins, etc., which promote skin metabolism and delay aging. Its mechanism of action is to inhibit melanin production by melanocytes and reduce melanin granules. Related studies have demonstrated that ginsenosides have whitening effects. Researchers used rats as experimental animals, with vitamin C as a control, to observe the inhibition rate of tyrosinase at the application sites. The study showed that the application sites of ginsenosides inhibited tyrosinase, further verifying the whitening effect of ginsenosides. Ginseng flower, also known as the divine herb flower, is the budding bud of ginseng, containing various saponin active substances, amino acids, and trace elements. Saffron (also known as Tibetan saffron) is a perennial stemless herb belonging to the Iridaceae family. The commercially valuable part of saffron is its stigmas. Each flower has only three stigmas, and it takes three years from seed to flowering, further enhancing its rarity. The chemical composition of saffron is 63% sugar, 12% protein, 10% water, 5% crude fiber, 5% fat, and 5% minerals. Its medicinal value depends on the presence of three main secondary metabolites: crocin, picrocrocin, and safranal. Saffron also has remarkable effects in skincare products. Natural pigments extracted from saffron, such as safflower yellow and safflower red, are safe for consumption and can be used as colorants in high-end cosmetics. Furthermore, saffron extract has blood-activating, anti-inflammatory, and antioxidant effects, improving skin blood circulation, promoting skin metabolism, scavenging free radicals, inhibiting melanin deposition, accelerating the fading of blemishes, and absorbing ultraviolet rays. Therefore, saffron plays a role in whitening, sun protection, and anti-aging in skincare products.

[0004] Existing technology CN116035975A discloses the application of peony flower water in skincare products. Specifically, it discloses peony petal water prepared using a compound microbial fermentation process, combined with a composition of apple juice, chamomile extract, and iris extract. This composition exhibits excellent antioxidant, whitening, and moisturizing effects, and also promotes the repair of skin barrier damaged by ultraviolet radiation. Existing technology CN111920040A discloses a peony fermentation product and its preparation method. It utilizes a mixture of at least one yeast and at least one lactic acid bacteria to ferment peony raw materials, obtaining the peony fermentation product. The yeast and lactic acid bacteria have a competitive yet interdependent relationship under symbiotic conditions; while competing for nutrients, they also produce substances needed by each other, thus enhancing the efficacy of the peony fermentation liquid.

[0005] However, with the continuous advancement of technology, emerging herbal exovesicle skincare products possess significant advantages in terms of bioactivity, safety, and transdermal absorption. Furthermore, compared to exosomes derived from animal cells, herbal exovesicles have a wider range of applications in the skincare field, making them more suitable for large-scale production and industrialization. Based on existing technologies, this invention focuses on researching the preparation of cosmetics from fermented plant extracts delivered via vesicles, and their efficacy. Summary of the Invention

[0006] This invention is based on the aforementioned known prior art and aims to provide a skin care composition for treating or preventing changes in skin pigmentation, fine lines, etc. caused by aging, and to provide a basic cosmetic and manufacturing method based on the composition.

[0007] Specifically, the present invention provides a three-flower color vesicle, characterized in that the vesicle contains a fermentation product obtained by mixing and fermenting peony petal extract, ginseng flower extract and saffron petal extract using a compound microbial agent.

[0008] Furthermore, the compound microbial agent is selected from one or more of the group consisting of Leuconostoc, Lactobacillus plantarum, and Rhodotorula rubrum.

[0009] Furthermore, the method for preparing the vesicles includes the following steps:

[0010] Step 1) Mix peony petal extract, ginseng flower extract and saffron petal extract, add distilled water, stir to homogenize, add activated compound microbial agent, ferment at 28-32℃ for 24-72h to obtain three-flower fermentation broth, centrifuge at 5000-10000g for 20-40min, collect the supernatant of fermentation broth, filter through filter membrane, collect the filtrate to obtain three-flower compound fermentation filtrate, collect the bacterial precipitate for later use;

[0011] Step 2) The bacterial cell precipitate from Step 1) is lysed using ultrasonic disruption to obtain a bacterial cell fragment solution. The fragment solution is then filtered through a filter membrane, and the filtrate is collected to obtain the three-flower color bacterial cell vesicles.

[0012] Step 3) Mix the Sanhua compound fermentation filtrate with Sanhua aerobic bacterial vesicles, sonicate, homogenize by high-speed microfluidic jet at 6000-10000psi pressure, cycle 4-6 times, and freeze dry to obtain Sanhua aerobic vesicles.

[0013] Furthermore, the mixing ratio of the peony petal extract, ginseng flower extract, and saffron petal extract is 5-15 parts by weight: 2-8 parts by weight: 2-8 parts by weight.

[0014] Furthermore, the compound microbial agent is composed of Leuconostoc mesenteroides, Lactobacillus plantarum, and Rhodotorula rubrum; wherein the inoculation concentration of Leuconostoc mesenteroides is 5.0 × 10⁻⁶. 6 -6.0×10 6 CFU / mL; the inoculum concentration of *Lactobacillus plantarum* was 5.0 × 10⁻⁶. 6 -6.5×10 6 CFU / mL; the inoculum concentration of red yeast was 4.0 × 10⁻⁶. 6 -6.0×10 6 cfu / mL.

[0015] Another aspect of the present invention provides a whitening and anti-aging composition containing peony, characterized in that the composition contains three-flower complexion vesicles.

[0016] Furthermore, the composition also contains butanediol.

[0017] Another aspect of the present invention provides the use of the said composition in the preparation of cosmetics.

[0018] Another aspect of the present invention provides a whitening and anti-aging cosmetic containing peony, characterized in that the cosmetic contains the aforementioned three-flower complexion vesicles.

[0019] Furthermore, the cosmetic product is selected from one or more dosage forms in the group consisting of lotion, toner, cream, facial mask, facial cleanser, body lotion, foundation, serum, massage cream, and hand cream.

[0020] In this invention, the activation method of Leuconostoc mesenteroides is a conventional method in the art, including but not limited to, picking a loopful of Leuconostoc mesenteroides colony and placing it in TYC medium, shaking and culturing at 30°C for 48 hours at a shaking speed of 150 r / min, with the preservation number of the strain being: CCTCDB20081674 or CCTCDB20082528.

[0021] The activation method of Lactobacillus plantarum is a conventional method in the field, including but not limited to picking a loopful of Lactobacillus plantarum colonies and placing them in MRS medium, incubating at 37°C with shaking for 48 hours at a shaking speed of 150 r / min, and the preservation number of the strain is: CCTCLB2008011 or CCTCLB2008009.

[0022] The activation method of red yeast is the conventional method in this field, including but not limited to, picking a loopful of Lactobacillus plantarum colony and placing it in MRS medium, shaking and culturing at 30°C for 48 hours at a shaking speed of 150 r / min, and the preservation number of the strain is: CCTCCAY2022008 or CCTCCAY2015004.

[0023] The peony petal extract, ginseng flower extract, and saffron petal extract in this invention are all extracted using conventional methods or are commercial products, such as those purchased from Xi'an Jincuifang Plant Technology Development Co., Ltd. and Guangdong Mingtong Biotechnology Co., Ltd., while arbutin, kojic acid, etc. are purchased from Xi'an Lvtian Biotechnology Co., Ltd., etc.

[0024] The beneficial effects of this invention include:

[0025] (1) The three-flower color vesicles prepared by the present invention have high safety, can effectively scavenge free radicals and effectively resist aging; in addition, at appropriate concentrations, they can achieve the same or better effect as arbutin or kojic acid in inhibiting melanin production in cells.

[0026] (2) The composition of the present invention has the effect of improving skin microcirculation, and also provides good support for maintaining the microecology of the skin surface and improving the skin barrier.

[0027] (3) The composition of the present invention has high stability and does not require the addition of antibacterial agents, providing an ideal raw material for the development of alternative additive-free cosmetics. Attached Figure Description

[0028] Figure 1 The results show the effect of the composition of the present invention on improving microcirculation. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention are within the scope of protection of the present invention.

[0030] Example 1

[0031] The steps for preparing the three-flower color vesicles include the following:

[0032] Step 1): Mix peony petal extract, ginseng flower extract, and saffron petal extract in a weight ratio of 10 parts: 5 parts: 5 parts. Add 5 times the volume of distilled water to the mixture, stir to homogenize, add the activated compound microbial agent, and ferment at 30°C for 48 hours to obtain the three-flower compound fermentation broth. Centrifuge at 8000g for 20 minutes at 4°C, and collect the supernatant and bacterial precipitate separately. Collect the supernatant, filter it through a membrane (Millipore 0.22-micron membrane), and collect the filtrate to obtain the three-flower compound fermentation filtrate. Collect the bacterial precipitate for later use. The inoculation density of the microorganisms in the compound microbial agent is as follows: Leuconostoc mesenteroides inoculation density is 5.5 × 10⁻⁶. 6The concentration of cfu / mL and the inoculation density of Lactobacillus plantarum were 6.0 × 10⁻⁶. 6 The inoculation density of Rhodotorula glutinis was 5.0 × 10⁻⁶ CFU / mL. 6 cfu / mL.

[0033] Step 2) The bacterial cell precipitate from Step 1) was lysed using ultrasonic disruption. The mixture was ultrasonicated at 4°C for 40 min on an ice bath and centrifuged at 5000g at 4°C for 30 min. The supernatant was collected to obtain a bacterial cell fragment solution. The fragment solution was filtered through a filter membrane (Millipore 0.22 μm filter membrane), and the filtrate was collected to obtain the three-flower color bacterial cell vesicles.

[0034] Step 3) The filtrate of the Sanhua compound fermentation was mixed with the Sanhua color-changing bacterial vesicles at a mass ratio of 2:1. After ultrasonic oscillation in a 37°C water bath for 1 hour, the mixture was homogenized by high-speed microfluidic jet at 8000 psi for 5 cycles at a temperature of 30°C. Then, the mixture was freeze-dried to obtain the Sanhua color-changing vesicles.

[0035] Safety assessment

[0036] The three-flower color-changing vesicles obtained in Example 1 and the composition prepared from them (containing 50% butylene glycol) were entrusted to an AAALAC-accredited evaluation company for safety assessment. The results showed that the total bacterial count of this product was <100 CFU / g, the total mold and yeast count was <10 CFU / g, thermotolerant coliforms were not detected, Staphylococcus aureus was not detected, and Pseudomonas aeruginosa was not detected; the lead content of this product was ≤10 mg / kg, mercury ≤1 mg / kg, arsenic ≤2 mg / kg, cadmium ≤5 mg / kg, and pesticide residues were not detected. The results indicate that no safety hazards will be generated in the raw materials, and under normal and reasonable use conditions, it will not cause harm to human health.

[0037] Comparative Example 1

[0038] See Example 1, but in step 1), only the peony petal extract and the saffron petal extract are mixed, and the specific mixing ratio is 10 parts by weight: 5 parts.

[0039] Comparative Example 2

[0040] See Example 1, but in step 1), only the peony petal extract and ginseng flower extract are mixed, and the specific mixing ratio is 10 parts by weight: 5 parts.

[0041] Comparative Example 3

[0042] See Example 1, but in step 1), peony petal extract, ginseng flower extract and saffron petal extract are mixed in a specific mixing ratio of 10 parts by weight: 2.5 parts by weight: 7.5 parts by weight.

[0043] Comparative Example 4

[0044] See Example 1, but in step 1), peony petal extract, ginseng flower extract and saffron petal extract are mixed in a specific mixing ratio of 10 parts by weight: 7.5 parts by weight: 2.5 parts by weight.

[0045] Free radical scavenging performance determination

[0046] The method is derived from "T / SHRH 006-2018 Cosmetics - Free Radical Scavenging Test Method". The principle is that DPPH is a stable, long-lived free radical. Its ethanol solution is deep purple and exhibits strong absorption near 517 nm. When a free radical scavenger is present, the light absorption of the DPPH ethanol solution weakens due to the pairing of single electrons with the DPPH free radical scavenger. The degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it accepts, thus allowing evaluation of the sample's ability to scavenge free radicals, i.e., the strength of its antioxidant activity. The measurement procedure is common knowledge in the field, including but not limited to: using 1.5 mL centrifuge tubes to set up the test sample tube (T), the test sample background (T0), the DPPH tube (C), and the solvent background (C0). Three parallel tubes are set up for each sample, and three parallel tubes are also set up for the DPPH tube. 0.1 mL of the same concentration (1% concentration) of sample solution is added to each of the test sample tubes and the test sample background. Water is added to all tubes to bring the total to 0.3 mL, and the mixture is thoroughly mixed. Add 0.1 mL of DPPH ethanol solution to the sample tubes and DPPH tubes. Replace the sample background and solvent background with 95% ethanol. Gently shake and let stand at room temperature for 5 min. Transfer 200 μL of each reaction solution into a 96-well plate and measure the absorbance at 517 nm using a microplate reader. Calculate the DPPH free radical scavenging rate using the following formula:

[0047] DPPH free radical scavenging rate (%) = (1- )×100%;

[0048] Wherein, T represents the absorbance of the sample tube, i.e., the absorbance of the solution after the sample reacts with DPPH; T0 represents the background absorbance of the sample; C represents the average of three absorbance readings of the DPPH tube, i.e., the absorbance of the DPPH solution without sample; and CO represents the background absorbance of the solvent. Statistical analysis is expressed as mean ± SD, and statistical analysis was performed using SPSS software (compared with the blank control group). The detection results are shown in Table 1.

[0049] Table 1. Test results of free radical scavenging performance

[0050] Test group DPPH free radical scavenging rate (%) p-value Test results Blank control group 0±0.187 - - Example 1 20.3±0.177 <0.01 Significant Comparative Example 1 15.2±0.248 <0.01 Significant Comparative Example 2 13.5±0.145 <0.01 Significant Comparative Example 3 19.1±0.033 <0.01 Significant Comparative Example 4 18.4±0.159 <0.01 Significant

[0051] As shown in Table 1, compared with the blank control group, the DPPH free radical scavenging rate of each group of the test samples increased significantly at a concentration of 1%, indicating that the samples have good antioxidant effects and the Sanhua Qise vesicle composition has a good synergistic effect. Further analysis showed that the scavenging rate of Example 1 was also significantly different from that of Comparative Example 1 and Comparative Example 2 (p<0.05), and the scavenging rate was the best under the formulation conditions of Example 1.

[0052] Example 2

[0053] The causes of melanin production on the skin surface are complex and diverse, mainly including genetic and environmental factors. A key challenge in developing skin-whitening skincare products is inhibiting the activity of tyrosinase in cells to reduce melanin formation and accumulation. Tyrosinase is a key enzyme in melanin biosynthesis, and its activity is correlated with melanin content. B-16 melanoma cells were prepared at a concentration of 5 × 10⁻⁶ cells / cells. 4 pcs / cm 2 Cells were seeded at a density of [missing information] in 6-well plates and cultured at 37°C with 5% CO2. After 1 day of static incubation, [missing information] were injected with 30 mJ / cm [missing information]. 2 The cells were irradiated with ultraviolet light (wavelength 280 nm) for 20 min, and then irradiated again for 20 min after 24 h. The supernatant was discarded, and the cells were grouped and treated. After 7 days, the same number of cells were scraped off and collected. After lysis with RIPA lysis buffer, the cells were centrifuged at 10000g for 20 min at 4℃. The cell pellet was collected and 500 μL of 1mol / L NaOH solution was added. After shaking and mixing, the cells were allowed to stand for 1 h and then centrifuged at 10000g for 15 min. The protein concentration of the supernatant was measured (Brandford method), and the absorbance was measured at a wavelength of 450 nm. The melanin inhibition rate (%) was calculated as [(w blank - w treatment) / w blank] × 100%, where w blank represents the mass fraction of melanin in the protein of the blank group and w treatment represents the mass fraction of melanin in the protein of the treated group. The results are shown in Table 2.

[0054] The method for grouping and processing cells is as follows:

[0055] Blank group: Add 1 mL of RPMI 1640 culture medium;

[0056] Stock solution group: Add 1 mL of RPMI1640 culture medium containing a mixture of peony petal extract, ginseng flower extract and saffron petal extract (in a weight ratio of 10:5:5) (concentration of 1 μg / mL).

[0057] Experimental group 1: Add 1 mL of RPMI 1640 culture medium containing the three-flower aerozoites of Example 1 (concentration 1 μg / mL).

[0058] Experimental group 2: Same as experimental group 1, but with a concentration of 5 μg / mL;

[0059] Experimental group 3: Same as experimental group 1, but with a concentration of 10 μg / mL;

[0060] Experimental group 4: Same as experimental group 1, but with a concentration of 20 μg / mL;

[0061] Experimental group 5: RPMI1640 culture medium containing triflora aerozoic bacterial vesicles, prepared at a concentration of 10 μg / mL;

[0062] Control group 1: Same as above, treated with 5 ppm β-arbutin;

[0063] Control group 2: Same as above, treated with 5 ppm kojic acid.

[0064] Table 2. Inhibition results (%) of melanin content in B-16 cells after each group of tests.

[0065] Group Melanin reduction Original solution group 9.2 Experimental group 1 13.8 Experimental group 2 18.7 Experimental group 3 51.8 Experimental group 4 53.0 Experimental group 5 2.1 Control group 1 49.3 Experimental group 2 45.9

[0066] Table 2 shows that when melanocytes were treated with *Trifolium repens* vesicles at concentrations of 10 μg / mL or higher, melanin production was significantly reduced in cells irradiated with ultraviolet light (p<0.05). Furthermore, at these concentrations and higher, *Trifolium repens* vesicles exhibited an inhibitory effect on melanin production comparable to or better than arbutin or kojic acid. This may be because the bioavailability and compatibility of the *Trifolium repens* plant extract improved after fermentation, resulting in better effects and indicating its promising application prospects for skin whitening.

[0067] Example 3

[0068] The blood flow velocity and volume in the skin's microcirculation are adapted to the metabolic levels of tissues and organs, ensuring the normal functioning of various organs within the body. Improving skin microcirculation can enhance skin cell activity, provide sufficient nutrients to skin cells, and simultaneously remove cellular metabolic waste and various harmful substances. In this embodiment, a blood flow analyzer was used to detect changes in blood flow velocity in zebrafish to evaluate the effect of the product of this invention on improving microcirculation.

[0069] The specific operation includes, but is not limited to, the following steps: After determining the maximum detectable concentration (1%) in zebrafish, the experimental system was further selected as wild-type AB strain zebrafish (age: 2 days post-fertilization, 2dpf; sample size per group: 30 fish, N=30). Zebrafish were randomly selected into 6-well plates, 30 fish per well, and water-soluble samples (three-flower color vesicles obtained in Example 1, prepared at a concentration of 1%) were administered. A normal control group was also set up. The volume of each well was 3 mL. The samples were incubated at 28°C in the dark for 24 hours. Ten zebrafish from each experimental group were randomly selected and placed in a blood flow and heart rate analyzer to record videos and collect data. The intersegmental blood flow velocity (V) of the zebrafish was analyzed. The calculation formula is as follows: Improved circulation (%) = (V of sample group - V of normal control group) / (V of normal control group) × 100%. The results are shown in Table 3. Figure 1 .

[0070] Table 3. Detection results of the effect on improving microcirculation (n=10)

[0071] Group Intersegmental vascular blood flow velocity (μm / s, mean±SE) Improvement of microcirculation (%) p-value Test results normal control group 362±114 - - - Three-flower complexion vesicle group 465±147 28 <0.001 Significant

[0072] As shown in Table 3, the three-flower color vesicles of the present invention significantly increased the change in intersegmental blood flow velocity compared with the normal control group, revealing that the composition of the present invention has a significant effect on improving skin microcirculation. Further research shows that the product of the present invention also has good support for maintaining the microecology of the skin surface, thereby improving the skin barrier.

[0073] Example 4

[0074] The three-flower color vesicles obtained in Example 1 of this invention were mixed with butylene glycol (50%) to prepare a three-flower color vesicle solution. The solution was stored under different storage conditions for 30 days, and the presence of discoloration, layering and off-odors was observed. The results are shown in Table 4.

[0075] Table 4 Stability Test Results

[0076] 4℃ 25℃ 37℃ 50℃ Odor none none A slightly pungent odor appeared after 15 days. An irritating odor appeared after 10 days. Layering none none none There will be stratification after 10 days. Color translucent, pale yellow translucent, pale yellow After 15 days, it becomes slightly cloudy and yellow. After 10 days, it became cloudy and dark yellow.

[0077] As shown in Table 4, the three-flower color-enhancing vesicle composition of the present invention exhibits high stability under low temperature or room temperature conditions, and its storage effect is better than that under high temperature conditions. When the same dosage of a mixture containing peony petal extract, ginseng flower extract, and saffron petal extract (in a weight ratio of 10:5:5) and 50% butylene glycol was prepared, observations under the above conditions revealed that the composition began to separate into layers on day 3 at all observed temperatures, and a slightly pungent odor appeared after day 10, with the color becoming a cloudy yellow. The possible reason is that the product of the present invention is a mixture of fermented organic matter and plant extracts, and through the loading of bacterial film vesicles, it can effectively maintain biological activity and product stability, while inhibiting microbial growth, providing a research foundation for the subsequent development of whitening and anti-aging products with reduced or no preservatives.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A whitening and anti-aging composition containing peony, characterized in that, The composition contains a tri-flower color vesicle, the vesicle contains a fermentation product obtained by mixed fermentation of peony petal extract, ginseng flower extract and saffron petal extract using a complex microbial agent including Leuconostoc, Lactobacillus plantarum and Rhodotorula, The preparation method of the vesicle includes the following steps: Step 1), mixing peony petal extract, ginseng flower extract and saffron petal extract, adding distilled water, stirring homogenate, adding activated complex microbial agent, 28-32℃ fermentation for 24-72h, obtaining tri-flower fermentation liquor, 5000-10000g centrifugation for 20-40min, collecting fermentation liquor supernatant, filtering through filter membrane, collecting filtrate to obtain tri-flower complex fermentation filtrate, collecting bacterial body precipitate for standby; Step 2), using ultrasonic crushing method to lyse the bacterial body precipitate in step 1), obtaining bacterial body fragment solution, filtering the fragment solution through filter membrane, collecting filtrate, obtaining tri-flower color bacterial body vesicle; Step 3), mixing tri-flower complex fermentation filtrate and tri-flower color bacterial body vesicle, ultrasonic oscillation, high-speed micro-jet homogenization under 6000-10000psi pressure, after 4-6 times of circulation, freeze-drying to obtain tri-flower color vesicle, The mixing ratio of the peony petal extract, ginseng flower extract and saffron petal extract is 5-15 parts by weight: 2-8 parts by weight: 2-8 parts by weight.

2. The use of the composition according to claim 1 in the preparation of a cosmetic for improving skin microcirculation.

3. The use of the composition according to claim 1 in the preparation of a cosmetic for inhibiting melanin production.

4. The composition of claim 1, wherein, The complex microbial agent is composed of Leuconostoc, Lactobacillus plantarum and Rhodotorula; wherein the inoculation concentration of Leuconostoc is 5.0×10 6 -6.0×10 6 cfu / mL; the inoculation concentration of Lactobacillus plantarum is 5.0×10 6 -6.5×10 6 cfu / mL; and the inoculation concentration of Rhodotorula is 4.0×10 6 -6.0×10 6 cfu / mL.

5. A whitening and anti-aging cosmetic containing Paeonia suffruticosa, characterized in that, The cosmetic contains the composition of claim 1.

6. The cosmetic product according to claim 5, characterized in that The cosmetic further contains butylene glycol.

7. The cosmetic product according to claim 6, characterized in that, The cosmetic is selected from one or more than one dosage form selected from the group consisting of skin toner, facial mask, facial cleansing cream, body milk, foundation, serum, hand cream.

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