CeO2 NPs-loaded hydrogel blood bag coating for blood storage and preparation method of CeO2 NPs-loaded hydrogel blood bag coating

Abstract

The invention discloses a CeO2NPs-loaded hydrogel blood bag coating for blood storage and a preparation method of the CeO2NPs-loaded hydrogel blood bag coating. The preparation method comprises the following steps: 1, preparing CeO2NPs; 2, preparing a hydrogel prepolymerization solution loaded with CeO2NPs; 3, performing air plasma treatment on the blood bag sheet; 4, preparing a silanization modified blood bag sheet; and 5, preparing the CeO2NPs-loaded hydrogel blood bag coating. According to the constructed hydrogel functional blood bag coating, the inner surface of a traditional blood bag is endowed with excellent biocompatibility, oxidation resistance, antibacterial performance and structural stability, the surface stress reaction caused by direct contact of red blood cells with the blood bag is effectively reduced, the storage microenvironment is improved, and the overall stability and safety of the red blood cells in the storage period are improved.

Description

Technical Field

[0001] This invention belongs to the field of biomaterials technology, specifically relating to a CeO2 NPs-loaded hydrogel blood bag coating for blood storage and its preparation method. Background Technology

[0002] The collected blood is stored in blood bags made of polyvinyl chloride (PVC). PVC is a soft, transparent, and durable plastic material with good biocompatibility and chemical resistance. A major storage injury to blood during storage in blood bags is oxidative stress. Oxidative stress occurs when the physiological balance between antioxidants and oxidants is disrupted. Under normal physiological conditions, a balance exists between the erythrocyte antioxidant system and free radicals. Under blood storage conditions, the blood antioxidant system may be unable to protect blood cells from oxidative damage by free radicals. During blood storage, decreased adenosine triphosphate (ATP) levels, decreased mitochondrial function, pH changes, and increased free radical oxidative stress lead to oxidative damage to erythrocyte membrane lipids and proteins, causing the erythrocyte cell membrane to harden or even deform, thus affecting the quality of the blood during storage.

[0003] Current improvements in blood storage primarily focus on optimizing and developing preservation solutions, intelligent management of storage conditions, optimization of blood bag materials and structures, and improvement of storage equipment. While progress has been made in optimizing preservation solution formulations, cryopreservation, and upgrading blood bag materials, preventing storage damage to red blood cells from the "source"—the materials in contact with the blood bag—remains a key challenge in blood storage research. As the direct carrier for red blood cell preservation, the material and surface properties of blood bags significantly influence the biocompatibility and stability of red blood cells during storage.

[0004] Therefore, there is a need to develop a blood bag that can improve the quality of blood storage and extend the storage time of blood as much as possible, and endow the inner surface of traditional blood bags with excellent biocompatibility, antioxidant properties and structural stability, thereby slowing down the damage and aging of red blood cells during storage. Summary of the Invention

[0005] The technical problem to be solved by this invention is to address the shortcomings of the prior art by providing a CeO2-loaded NPs hydrogel coating for blood storage and its preparation method. This coating can improve the microenvironment of the inner surface of the blood bag, thereby delaying red blood cell storage damage, improving blood storage quality, and extending blood storage time.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is: a method for preparing a CeO2NPs-loaded hydrogel blood bag coating for blood storage, characterized by comprising the following steps:

[0007] Step 1: Add cerium chloride solution to sodium hydroxide solution, sonicate and centrifuge to discard the supernatant, wash with deionized water, and pre-freeze the resulting purplish-gray cerium dioxide nanomaterial suspension. Then vacuum dry to obtain purplish-gray CeO2 NPs.

[0008] Step 2: Add polyvinylpyrrolidone and the CeO2 NPs described in Step 1 to PBS buffer, stir, and sonicate to uniformly disperse and dissolve the CeO2 NPs to obtain solution A; add acrylamide, quaternary ammonium salt chitosan, and sulfobetaine methacrylate to PBS buffer and mix well to obtain solution B; mix solutions A and B, then add crosslinking agent and stir, then add initiator and stir, and sonicate to obtain CeO2 NPs-loaded hydrogel prepolymer;

[0009] Step 3: Clean the blood bag sheets with water and ethanol using ultrasonic cleaning, air dry them naturally, and then treat them with air plasma.

[0010] Step 4: Prepare MAPTMS ethanol solution, adjust pH with acetic acid, and perform catalytic hydrolysis at room temperature to obtain MAPTMS solution; immerse the blood bag sheet treated with air plasma in Step 3 in MAPTMS solution for 30 min to 60 min, and dry to obtain silanized modified blood bag sheet.

[0011] Step 5: Uniformly coat the CeO2 NPs-loaded hydrogel prepolymer solution described in Step 2 onto the modified blood bag sheet described in Step 4, cure with UV for 3 to 8 minutes, and heat to cure the coating to obtain the CeO2 NPs-loaded hydrogel blood bag coating.

[0012] The method for preparing a CeO2 NPs-loaded hydrogel blood bag coating for blood storage is characterized in that the molar ratio of cerium chloride and sodium hydroxide in step one is 1:1.

[0013] The method for preparing a CeO2 NPs-loaded hydrogel blood bag coating for blood storage is characterized in that the concentration of the cerium chloride solution in step one is 0.1 mol / L to 0.5 mol / L, and the concentration of the sodium hydroxide solution is 0.1 mol / L to 0.5 mol / L.

[0014] The method for preparing a CeO2 NPs-loaded hydrogel blood bag coating for blood storage is characterized in that, in step two, the concentration of CeO2 NPs in solution A is 0.0025 g / mL to 0.025 g / mL, and the concentration of polyvinylpyrrolidone is 0.05 g / mL to 0.2 g / mL; the mass concentration of acrylamide in solution B is 15% to 25%, the mass concentration of quaternary ammonium chitosan is 2% to 4%, and the mass concentration of sulfobetaine methacrylate is 2% to 4%.

[0015] The above-mentioned method for preparing a CeO2 NPs-loaded hydrogel blood bag coating for blood storage is characterized in that the crosslinking agent in step two is N,N'-methylenebisacrylamide, the initiator is ammonium persulfate, the concentration of the crosslinking agent in the CeO2 NPs-loaded hydrogel prepolymer solution is 3 mg / mL to 5 mg / mL, and the concentration of the initiator is 2 mg / mL to 4 mg / mL.

[0016] The method for preparing a CeO2 NPs-loaded hydrogel blood bag coating for blood storage is characterized in that the volume ratio of solution A and solution B in step two is 1:(1-3).

[0017] The method for preparing a CeO2 NPs-loaded hydrogel blood bag coating for blood storage is characterized in that, in step four, the concentration of MAPTMS in the MAPTMS ethanol solution is 0.21M to 0.84M, and the pH is adjusted to 4.5 to 5 with acetic acid.

[0018] The method for preparing a CeO2 NPs-loaded hydrogel blood bag coating for blood storage is characterized in that the drying temperature in step four is 60℃~80℃ and the drying time is 1h~5h.

[0019] The above-mentioned method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage is characterized in that, in step five, each cm 2 Modified blood bag sheets are prepared using 5 μL to 10 μL of CeO2 NPs hydrogel prepolymer solution, with a heating temperature of 40℃ to 60℃ and a curing time of 1 h to 3 h.

[0020] Furthermore, the present invention provides a CeO2 NPs hydrogel blood bag coating for blood storage prepared by the above method.

[0021] Compared with the prior art, the present invention has the following advantages:

[0022] 1. This invention applies a multifunctional synergistic hydrogel system to regulate the microenvironment of erythrocyte storage, while loading CeO2NPs, which can effectively remove ROS during the blood storage process, alleviate oxidative damage to erythrocytes, and form a hydrogel system with "antioxidant + anti-pollution + flexible interface buffer".

[0023] 2. The CeO2 NPs hydrogel blood bag coating of the present invention for blood storage has good biocompatibility and antibacterial properties.

[0024] 3. The CeO2-loaded NPs hydrogel blood bag coating prepared by the method of the present invention has abundant and readily available raw materials and low cost; the synthesis process is simple and easy to implement.

[0025] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0026] Figure 1 This is a TEM image of CeO2 NPs in Embodiment 1-1 of the present invention.

[0027] Figure 2 This is a particle size diagram of CeO2 NPs in Example 1-1 of the present invention.

[0028] Figure 3 The image shows the XRD pattern of CeO2 NPs in Embodiment 1-1 of the present invention.

[0029] Figure 4 The infrared spectrum of the CeO2NPs-loaded hydrogel of Example 2-1 of this invention is shown.

[0030] Figure 5 This is a graph showing the cytotoxicity results of L929 cells using CeO2NPs hydrogel in Example 2-1 of this invention.

[0031] Figure 6 This is an AFM image of the CeO2NPs-loaded hydrogel blood bag coating from Example 3-1 of the present invention.

[0032] Figure 7 This is a graph showing the blood compatibility results of the CeO2NPs-loaded hydrogel blood bag coating in Example 3-1 of the present invention. Detailed Implementation

[0033] The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0034] Unless otherwise defined, the technical terms used in the following embodiments have the same meanings as commonly understood by those skilled in the art to which this invention pertains. Unless otherwise specified, the experimental reagents used in the following embodiments are conventional biochemical reagents; and the experimental methods described are conventional methods.

[0035] Example 1-1: Preparation of CeO2 NPs

[0036] Weigh out 0.042 g of sodium hydroxide and 0.374 g of cerium chloride and dissolve them separately in 10 mL of deionized water. Add the cerium chloride solution to the sodium hydroxide solution to obtain a suspension. Wash the suspension with deionized water and centrifuge. Pre-freeze the resulting purplish-gray cerium dioxide nanomaterial suspension and place it in a vacuum freeze dryer to dry it under vacuum to obtain the desired purplish-gray CeO2NPs.

[0037] like Figure 1 As shown in the transmission electron microscopy (TEM) image of the CeO2 NPs prepared in this embodiment, the product is observed to be a uniformly shaped sphere. Figure 2 It can be seen that the particle size of CeO2 NPs is about 100 nm. Figure 3 The XRD pattern of this substance shows that the peak positions match those on the standard card, further confirming that the generated sample is CeO2.

[0038] Examples 1-2: Preparation of CeO2 NPs

[0039] Weigh out 0.08 g of sodium hydroxide and 0.746 g of cerium chloride and dissolve them separately in 10 mL of deionized water. Add the cerium chloride solution to the sodium hydroxide solution to obtain a suspension. Sonicate the suspension, wash it with deionized water, and centrifuge. Pre-freeze the resulting purplish-gray cerium dioxide nanomaterial suspension and place it in a vacuum freeze dryer to dry it under vacuum to obtain the desired purplish-gray CeO2NPs.

[0040] The morphology, particle size, and other physicochemical properties of CeO2 NPs in this embodiment are basically the same as those in Example 1-1.

[0041] Examples 1-3: Preparation of CeO2 NPs

[0042] Weigh out 0.4 g of sodium hydroxide and 3.73 g of cerium chloride and dissolve them separately in 20 mL of deionized water. Add the cerium chloride solution to the sodium hydroxide solution to obtain a suspension. Sonicate the suspension, wash it with deionized water, and centrifuge it. Pre-freeze the resulting purplish-gray cerium dioxide nanomaterial suspension and place it in a vacuum freeze dryer to dry it under vacuum to obtain the desired purplish-gray CeO2NPs.

[0043] The morphology, particle size, and other physicochemical properties of CeO2 NPs in this embodiment are basically the same as those in Example 1-1.

[0044] Example 2-1: Preparation of CeO2-loaded NPs hydrogel prepolymer solution

[0045] Step 1: Add 0.02g of CeO2 NPs prepared in Example 1-1 and 0.2g of polyvinylpyrrolidone to 2mL of PBS buffer, stir and sonicate to uniformly disperse CeO2 NPs, and obtain solution A;

[0046] Step 2: Add acrylamide (AAM), quaternary ammonium chitosan (QCS), and sulfobetaine methacrylate (SBMA) to 4 mL of PBS buffer and stir to obtain solution B. The mass concentration of acrylamide in the solution is 20%, the mass concentration of quaternary ammonium chitosan is 3%, and the mass concentration of sulfobetaine methacrylate is 3%.

[0047] Step 3: Mix solution A and solution B at a volume ratio of 1:2, then add crosslinking agent N,N'-methylenebisacrylamide and stir for 15 min. Then add initiator ammonium persulfate and stir. Sonicate to obtain CeO2 NPs hydrogel prepolymer solution. The crosslinking agent concentration in the hydrogel prepolymer solution is 4 mg / mL and the initiator concentration is 3 mg / mL.

[0048] Infrared detection was performed on the CeO2-loaded NPs hydrogel prepared in this embodiment, and the results are as follows: Figure 4 As shown, the infrared spectrum of the product indicates that at 550 cm⁻¹... -1 The characteristic peak at 1239 cm⁻¹ is a feature of -Ce-O, indicating successful loading of CeO₂ NPs onto the surface. -1 The peak at 1673 cm⁻¹ represents the stretching vibration of the -S=O group, while the peak at 1673 cm⁻¹ represents the stretching vibration of the -S=O group. -1 The peak value originated from the -C=O gene, thus confirming the successful preparation of the hydrogel.

[0049] Example 2-2: Preparation of CeO2-loaded NPs hydrogel prepolymer solution

[0050] Step 1: Add 0.005g of CeO2 NPs prepared in Examples 1-2 and 0.1g of polyvinylpyrrolidone to 2mL of PBS buffer, stir and sonicate to uniformly disperse the CeO2 NPs, and obtain solution A;

[0051] Step 2: Add acrylamide (AAM), quaternary ammonium chitosan (QCS), and sulfobetaine methacrylate (SBMA) to 4 mL of PBS buffer and stir to obtain solution B. The mass concentration of acrylamide in the solution is 15%, the mass concentration of quaternary ammonium chitosan is 2%, and the mass concentration of sulfobetaine methacrylate is 2%.

[0052] Step 3: Mix solution A and solution B in a 1:1 volume ratio, then add crosslinking agent N,N'-methylenebisacrylamide and stir for 15 min. Then add initiator ammonium persulfate and stir. Sonicate to obtain CeO2 NPs hydrogel prepolymer solution. The crosslinking agent concentration in the hydrogel prepolymer solution is 3 mg / mL and the initiator concentration is 2 mg / mL.

[0053] The performance of the CeO2-loaded NPs hydrogel in this embodiment is basically the same as that in Example 2-1.

[0054] Examples 2-3: Preparation of CeO2-loaded NPs hydrogel prepolymer solution

[0055] Step 1: Add 0.05g of CeO2 NPs prepared in Examples 1-3 and 0.4g of polyvinylpyrrolidone to 2mL of PBS buffer, stir and sonicate to uniformly disperse the CeO2 NPs, and obtain solution A;

[0056] Step 2: Add acrylamide (AAM), quaternary ammonium chitosan (QCS), and sulfobetaine methacrylate (SBMA) to 4 mL of PBS buffer and stir to obtain solution B. The mass concentration of acrylamide in the solution is 25%, the mass concentration of quaternary ammonium chitosan is 4%, and the mass concentration of sulfobetaine methacrylate is 4%.

[0057] Step 3: Mix solution A and solution B at a volume ratio of 1:3, then add crosslinking agent N,N'-methylenebisacrylamide and stir for 15 min. Then add initiator ammonium persulfate and stir. Sonicate to obtain CeO2 NPs hydrogel prepolymer solution. The crosslinking agent concentration in the hydrogel prepolymer solution is 5 mg / mL and the initiator concentration is 4 mg / mL.

[0058] The performance of the CeO2-loaded NPs hydrogel in this embodiment is basically the same as that in Example 2-1.

[0059] Cytotoxicity assay of CeO2 NPs hydrogel

[0060] After sterilizing an appropriate amount of the CeO2-loaded NPs hydrogel prepared in Example 2-1, disperse it at a concentration of 0.1 g / mL in 1640 complete culture medium (containing 10% serum and 1% penicillin antibiotics). Incubate at 37°C for 72 h, filter through a 0.22 μm filter membrane, and dilute to different concentrations to obtain the hydrogel sample extract. Seal the extract and store at 4°C for later use. In 96-well plates, culture mouse fibroblasts (L929 cells) in complete culture medium until cell adhesion occurs. Replace the original culture medium with the above sample extract. For the control group, replace the original culture medium with 1640 complete culture medium. Continue culturing for 24 h and 48 h, then add 10 μL of LCK-8 solution to each well and culture for another 1–2 h. After culture, measure the absorbance (OD value) of each group at 450 nm using a microplate reader. Cell viability is calculated using the following formula: Cell viability (%) = [(sample A - empty A) / (pair A - empty A)] × 100%, where sample A is the absorbance of the test sample, empty A is the absorbance of the culture medium, and pair A is the absorbance of the control group.

[0061] Figure 5The results show the cytotoxicity of the CeO2 NPs-loaded hydrogel described in Example 2-1 against mouse fibroblasts (L929 cells). After culturing the extract of the CeO2 NPs-loaded hydrogel prepared in Example 2-1 for 24 h or 48 h, the cell viability of L929 cells was higher than 85%, indicating that the CeO2 NPs-loaded hydrogel extract had no significant toxic side effects on L929 cells.

[0062] Example 3-1: Preparation of CeO2-loaded NPs hydrogel blood bag coating

[0063] Step 1: Clean the blood bag sheet (PVC) twice each with water and ethanol using ultrasonic cleaning, air dry naturally, and then treat it with air plasma.

[0064] Step 2: Prepare a MAPTMS ethanol solution with a concentration of 0.5M. Adjust the pH to 4.8 with acetic acid and perform catalytic hydrolysis at room temperature to obtain a MAPTMS solution. Immerse the blood bag sheet treated with air plasma in Step 1 in the MAPTMS solution for 50 minutes and dry it at 70°C for 3 hours to obtain silanized modified blood bag sheet.

[0065] Step 3: Uniformly coat 200 μL of the hydrogel prepolymer solution prepared in Example 2-1 onto a 25 cm² surface. 2 On the silanized modified blood bag sheet obtained in step two, UV curing was applied for 5 minutes, followed by heating at 50°C for 2 hours to cure the coating, resulting in a CeO2 NPs-loaded hydrogel blood bag coating.

[0066] Blood compatibility test of CeO2 NPs hydrogel blood bag coating

[0067] The CeO2-loaded NPs hydrogel blood bag coating prepared in Example 3-1 was cut and sterilized. The sterilized sample was then cut into 1.25 cm sections. 2 After immersion in physiological saline at an area-to-volume ratio of / mL for 24h, the sample was filtered through a 0.22μm filter membrane. Fresh rabbit whole blood was centrifuged at 2000 rpm for 5 minutes to obtain red blood cells, which were then diluted with physiological saline to 2% (v / v). An equal volume of red blood cell suspension and sample extract was added to centrifuge tubes, with a positive control (deionized water) and a negative control (physiological saline) set up. The tubes were incubated at 37℃ for 1 hour. After centrifugation, the absorbance of the supernatant at 540nm was measured and photographed.

[0068] Figure 6 The images show the AFM diagrams of the CeO2-loaded NPs hydrogel blood bag coating (PMSA@CeO2) prepared in this embodiment and the untreated blood bag PVC sheet. Figure 6It can be seen that the treated CeO2-loaded NPs hydrogel blood bag coating is smoother and has lower roughness than the untreated blood bag PVC sheet, which helps to reduce blood adhesion.

[0069] Figure 7 The CeO2-loaded NPs hydrogel blood bag coating prepared in this embodiment (Figure)

[0070] Results of blood compatibility analysis of PMSA@CeO2 and PVC sheet blood bags. The quantitative analysis results in the figure show that when the hemolysis rate of the saline group was 0%, the hemolysis rate of the blood bag materials was less than 5%; this result indicates that the CeO2 NPs-loaded hydrogel blood bag coating prepared in this embodiment has good blood compatibility.

[0071] Example 3-2: Preparation of CeO2-loaded NPs hydrogel blood bag coating

[0072] Step 1: Clean the blood bag sheet (PVC) twice each with water and ethanol using ultrasonic cleaning, air dry naturally, and then treat it with air plasma.

[0073] Step 2: Prepare a MAPTMS ethanol solution with a concentration of 0.21M. Adjust the pH to 5 with acetic acid and perform catalytic hydrolysis at room temperature to obtain a MAPTMS solution. Immerse the blood bag sheet treated with air plasma in Step 1 in the MAPTMS solution for 60 minutes and dry it at 60°C for 5 hours to obtain silanized modified blood bag sheet.

[0074] Step 3: Uniformly coat 125 μL of the hydrogel prepolymer solution prepared in Example 2-1 onto a 25 cm² surface. 2 On the silanized modified blood bag sheet obtained in step two, UV curing for 3 minutes and heating at 60°C for 1 hour are used to cure the coating, resulting in a CeO2 NPs-loaded hydrogel blood bag coating.

[0075] Example 3-3: Preparation of CeO2-loaded NPs hydrogel blood bag coating

[0076] Step 1: Clean the blood bag sheet (PVC) twice each with water and ethanol using ultrasonic cleaning, air dry naturally, and then treat it with air plasma.

[0077] Step 2: Prepare a MAPTMS ethanol solution with a concentration of 0.84 M. Adjust the pH to 4.5 with acetic acid and perform catalytic hydrolysis at room temperature to obtain a MAPTMS solution. Immerse the blood bag sheet treated with air plasma in Step 1 in the MAPTMS solution for 30 min and dry it at 80 °C for 1 h to obtain silanized modified blood bag sheet.

[0078] Step 3: Uniformly coat 250 μL of the hydrogel prepolymer solution prepared in Example 2-1 onto a 25 cm² surface. 2 On the silanized modified blood bag sheet obtained in step two, UV curing for 8 minutes and heating at 40°C for 3 hours were used to cure the coating, resulting in a CeO2 NPs-loaded hydrogel blood bag coating.

[0079] Blood compatibility tests were conducted on the CeO2 NPs-loaded hydrogel blood bag coatings prepared in Examples 3-2 and 3-3. The results were the same as those in Example 3-1. The CeO2 NPs-loaded hydrogel blood bag coatings were smoother and had lower roughness than the untreated PVC blood bag sheet, which helped to reduce blood adhesion. The hemolysis rate of the CeO2 NPs-loaded hydrogel blood bag coatings was less than 5%, indicating good blood compatibility.

[0080] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation on the present invention. Any simple modifications, alterations, or equivalent structural changes made to the above embodiments based on the technical essence of the invention shall still fall within the protection scope of the present invention.

Claims

1. A method for preparing a CeO2-loaded NPs hydrogel coating for blood storage, characterized in that, Includes the following steps: Step 1: Add cerium chloride solution to sodium hydroxide solution, sonicate and centrifuge to discard the supernatant, wash with deionized water, and pre-freeze the resulting purplish-gray cerium dioxide nanomaterial suspension. Then vacuum dry to obtain purplish-gray CeO2 NPs. Step 2: Add polyvinylpyrrolidone and the CeO2 NPs described in Step 1 to PBS buffer, stir, and sonicate to uniformly disperse and dissolve the CeO2 NPs to obtain solution A; add acrylamide, quaternary ammonium salt chitosan, and sulfobetaine methacrylate to PBS buffer and mix well to obtain solution B; mix solutions A and B, then add crosslinking agent and stir, then add initiator and stir, and sonicate to obtain CeO2 NPs-loaded hydrogel prepolymer; Step 3: Clean the blood bag sheets with water and ethanol using ultrasonic cleaning, air dry them naturally, and then treat them with air plasma. Step 4: Prepare a MAPTMS ethanol solution, adjust the pH with acetic acid, and perform catalytic hydrolysis at room temperature to obtain a MAPTMS solution. The blood bag sheet treated with air plasma in step three was immersed in MAPTMS solution for 30 min to 60 min and then dried to obtain silanized modified blood bag sheet. Step 5: Uniformly coat the CeO2 NPs-loaded hydrogel prepolymer solution described in Step 2 onto the modified blood bag sheet described in Step 4, cure with UV for 3 to 8 minutes, and heat to cure the coating to obtain the CeO2 NPs-loaded hydrogel blood bag coating.

2. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, In step one, the molar ratio of cerium chloride to sodium hydroxide is 1:

1.

3. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, The concentration of the cerium chloride solution in step one is 0.1 mol / L to 0.5 mol / L, and the concentration of the sodium hydroxide solution is 0.1 mol / L to 0.5 mol / L.

4. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, In step two, the concentration of CeO2 NPs in solution A is 0.0025 g / mL to 0.025 g / mL, and the concentration of polyvinylpyrrolidone is 0.05 g / mL to 0.2 g / mL; the mass concentration of acrylamide in solution B is 15% to 25%, the mass concentration of quaternary ammonium chitosan is 2% to 4%, and the mass concentration of sulfobetaine methacrylate is 2% to 4%.

5. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, The crosslinking agent mentioned in step two is N,N'-methylenebisacrylamide, the initiator is ammonium persulfate, and the concentration of the crosslinking agent in the CeO2NPs hydrogel prepolymer solution is 3 mg / mL to 5 mg / mL, and the concentration of the initiator is 2 mg / mL to 4 mg / mL.

6. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, The volume ratio of solution A to solution B in step two is 1:(1-3).

7. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, In step four, the concentration of MAPTMS in the MAPTMS ethanol solution is 0.21M to 0.84M, and the pH is adjusted to 4.5 to 5 with acetic acid.

8. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, The drying temperature in step four is 60℃~80℃, and the drying time is 1h~5h.

9. The method for preparing a CeO2-loaded NPs hydrogel blood bag coating for blood storage according to claim 1, characterized in that, In step five, each cm 2 The modified blood bag sheet is prepared using 5 μL to 10 μL of CeO2NPs hydrogel prepolymer solution, and the curing temperature is 40℃ to 60℃ for 1 to 3 hours.

10. A CeO2 NPs-loaded hydrogel blood bag coating for blood storage prepared by the method of any one of claims 1-9.

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