A method for preparing macrophage membrane-coated artificial cells

By constructing PDDA/SBE-β-CD condensate droplets through liquid-liquid phase separation and coating macrophage membranes, the problem of structural instability of membrane materials under high shear stress was solved, and the stability and biocompatibility of artificial cells were improved.

CN121182744BActive Publication Date: 2026-03-20NANCHANG UNIV
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Patent Information

Application Number
CN202511735887.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-11-25
Publication Date
2026-03-20
Estimated Expiration
2045-11-25

AI Technical Summary

Technical Problem

Existing technologies make it difficult to apply membrane modification techniques to the preparation of membrane-coated membranes, especially the preparation of artificial cells coated with macrophage membranes. There are problems such as the instability of membrane materials under high shear stress, which can lead to leakage of contents or uncontrollable fusion with the membrane material.

Method used

PDDA/SBE-β-CD condensate droplets were constructed using liquid-liquid phase separation technology, and macrophage membranes were coated onto their exteriors using a 4℃ low-temperature, intermittent ultrasonic encapsulation method to prepare macrophage membrane-coated artificial cells.

Benefits of technology

The constructed PDDA/SBE-β-CD condensate droplets are stable under physiological conditions and can rapidly and efficiently enrich dyes with diverse physicochemical properties. Furthermore, the macrophage membrane serves as an outer shell, which enhances structural stability and biocompatibility.

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Abstract

The application discloses a preparation method of macrophage membrane coated artificial cells and belongs to the technical field of artificial cell preparation. Polydiallyldimethylammonium chloride and sulfobutyl-beta-cyclodextrin are dissolved in ultrapure water, and the aqueous solutions of the two are mixed to obtain a PDDA / SBE-beta-CD condensate droplet solution; equal-mass-ratio macrophage membrane suspension liquid and the PDDA / SBE-beta-CD condensate droplet solution are mixed in equal ratio, in-situ coating of the macrophage membrane is realized by using ultrasonic coating technology, and macrophage membrane coated artificial cells are obtained. The PDDA / SBE-beta-CD condensate droplet constructed by the application has good stability under physiological salt concentration, pH conditions and 37 DEG C; the macrophage membrane coated artificial cells are prepared by mixing the macrophage membrane suspension liquid, and the macrophage membrane as the shell can not only prevent the condensate from gathering, but also increase the structural stability and biocompatibility.
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