Pesticide fertilizer for preventing and treating corn stem rot
By combining isofentrifluconazole and fluopyram with fertilizer to form a granular pesticide-fertilizer mixture, the risks of drug resistance and environmental pollution associated with using single fungicides to control corn stalk rot are solved, achieving the dual effects of efficient prevention and control and nutritional supplementation.
Patent Information
- Application Number
- CN202511373545.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-25
- Publication Date
- 2025-12-26
AI Technical Summary
Long-term and extensive use of single-component fungicides to control corn stalk rot poses risks of high resistance, environmental pollution, and pesticide residues. Furthermore, there are no reports of using isofentrifluconazole and fluopyram to prepare granular fertilizer formulations.
Ifentrifluconazole and fluopyram at a mass ratio of 1-9:9-1 are combined as the active ingredients of the pesticide and combined with fertilizer to form a pesticide-fertilizer granule. The specific components include 3% ifentrifluconazole, 1% fluopyram, 45% fertilizer, 3% binder, 1.5% dispersant, and inert filler to make up to 100%. The inert filler is a mixture of attapulgite, diatomaceous earth and kaolin in a ratio of 1:3:1.
It achieves highly efficient control of corn stalk rot, reduces the risk of drug resistance, combines the effects of medicine and fertilizer, promotes plant growth and improves stress resistance, and has dual effects with a single application.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of pesticides, and particularly relates to a medicine and fertilizer for preventing and treating corn stalk rot. BACKGROUND
[0002] Corn stalk rot is a typical soil-borne fungal disease caused by a variety of pathogenic fungi alone or in combination. The main pathogenic fungi of fungal stalk rot are Fusarium graminearum and Fusarium pseudograminearum. These pathogenic fungi begin to invade the plant roots and then enter the plant vascular system. As the vascular system spreads to various tissues of the plant, the plant aboveground tissues begin to rot when the mycelium gathers into a mycelial network, leading to premature wilting of the corn plant, stem breakage and ear drop, and significantly reducing the yield of corn.
[0003] Chemical fungicides are the main measure for preventing and treating corn stalk rot at present, which can reduce or alleviate the incidence of corn stalk rot. Common fungicides include triazolone, carbendazim, hymexazol, myclosin, tebuconazole and metalaxyl-M. Although chemical fungicides have the characteristics of convenient application and rapid effect, if a single component fungicide is used for a long time, the risk of drug resistance is high, which can easily cause the generation and accumulation of drug resistance, and also causes environmental pollution and pesticide residues. Therefore, it is of great significance to find new and efficient compound fungicides for preventing and treating corn stalk rot for corn disease control.
[0004] Ipfentrifluconazole is a new triazole fungicide developed by BASF, with the molecular formula: C 20 H 19 ClF3N3O2, CAS: 1417782-08-1, containing one asymmetric chiral carbon (C) atom, composed of 1 pair of enantiomers. Ipfentrifluconazole inhibits the biosynthesis of ergosterol in pathogenic fungi, which damages the function of fungal cell membrane, and then inhibits or interferes with the development of appressorium and haustoria, the formation of mycelium and spores, and can effectively prevent and control various fungal diseases such as scab, leaf spot and powdery mildew.
[0005] Medicine and fertilizer granules are a kind of functional preparation combining pesticides and fertilizers, which can reduce the amount of pesticides, improve the prevention effect, promote plant growth and improve plant stress resistance, save time and labor, and are currently a hot spot in the field of disease, pest and weed control research. However, not all combinations of any pesticide and fertilizer can produce the effect of medicine and fertilizer. The design of medicine and fertilizer granules needs to consider the synergistic effect of pesticide and fertilizer, and avoid the antagonistic effect between the components of medicine and fertilizer.
[0006] There is no related report on the preparation of medicine and fertilizer granules from ipfentrifluconazole and fluazinam.
[0007] The information disclosed in this Background section is only for the purpose of increasing an understanding of the general context of the present application and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art that is already known in any country in the world. SUMMARY
[0008] The present application aims to provide a medicine and fertilizer for preventing and treating corn stalk rot, so as to solve the problems existing in the long-term use of single-component fungicides.
[0009] To achieve the above-mentioned purpose, the present application provides the following technical solutions. A pesticide active ingredient, which is compounded by triflumizole and pyraclostrobin in a mass ratio of 1-9:9-1.
[0010] More specifically, the mass ratio of triflumizole to pyraclostrobin is 3:1.
[0011] The present application also provides a medicine and fertilizer granule, which comprises the pesticide active ingredient.
[0012] More specifically, the medicine and fertilizer granule is composed of the following components in mass percentage: triflumizole 1-5%, pyraclostrobin 0.5-2%, fertilizer 15-45%, binder 2-5%, dispersant 1-3%, and inert filler to make up to 100%.
[0013] More specifically, the medicine and fertilizer granule is composed of the following components in mass percentage: triflumizole 3%, pyraclostrobin 1%, fertilizer 45%, binder 3%, dispersant 1.5%, and inert filler to make up to 100%.
[0014] More specifically, the fertilizer is mixed by monoammonium phosphate, ammonium sulfate and potassium sulfate in a mass ratio of 3:7:5.
[0015] More specifically, the binder is hydroxypropyl methyl cellulose.
[0016] More specifically, the dispersant is sodium dodecyl sulfate.
[0017] More specifically, the inert filler is mixed by attapulgite, diatomite and kaolin in a mass ratio of 1:3:1.
[0018] Compared with the prior art, the present application has the following beneficial effects: (1) The present application compounds triflumizole and pyraclostrobin into a pesticide active ingredient with fungicidal efficacy, which shows a synergistic effect on Fusarium graminearum causing corn stalk rot, can improve the prevention and treatment effect on corn stalk rot, reduce the risk of drug resistance of the pesticide, and can provide support for the development of new high-efficiency fungicide for preventing and treating corn stalk rot.
[0019] (2) The present application combines the pesticide active ingredient prepared by compounding ifentride with pyraclostrobin and the fertilizer to form a functional pesticide fertilizer granule, which has both pesticide effect and fertilizer effect, improves the disease control effect, can reduce the application amount of pesticide, at the same time can supplement the nutrient substances required for the growth of corn plants, promotes the growth of plants and improves the stress resistance, one-time application, gets the double effects of pesticide and fertilizer, and has good application prospect. DETAILED DESCRIPTION
[0020] The following clearly and completely describes the technical solutions of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0021] In the following examples, hydroxypropyl methylcellulose (HPMC, Aldrich, Catalog No. H108825) was used.
[0022] Example 1: Indoor activity test of the compounded pesticide 1. Test strain and spore suspension Fusarium graminearum (Schwein) was isolated from diseased corn plant samples. Fusarium graminearum
[0023] Fusarium graminearum was inoculated on PDA medium and cultured at 28°C in the dark for 6 days. A 5mm diameter fungus cake was punched along the edge of the colony, and 5 fungus cakes were inoculated into 250mL of PDA liquid medium, and cultured at 200r / min and 28°C for 7 days. The supernatant was removed by filtering with double-layer gauze and centrifuging at 1000r / min for 5min, and deionized water was added for centrifugation again. A spore suspension of 1.0×10 7
[0024] PDA medium: 200g of peeled potatoes, 20g of glucose, 17g of agar, and distilled water to 1L, natural pH value; PDA liquid medium: 200g of peeled potatoes, 20g of glucose, and distilled water to 1L, natural pH value.
[0025] 2. Test agent 98% ifentride (Zhengzhou Huiju Chemical Co., Ltd.), 98% pyraclostrobin (Liaoning Youchuang Plant Protection Co., Ltd.) The test agent was dissolved in solvent and then diluted with 0.1% Tween-80 aqueous solution to prepare a high-concentration single-agent stock solution of 5000 mg / L. According to the results of the preliminary test, multiple groups of formulations were prepared by diluting the single-agent stock solutions and each group of mixed agent with 0.1% Tween-80 aqueous solution to 5 concentration gradients. The above agents were prepared immediately before use.
[0026] 3. Test method The effects of ifenprodil triflumizole and pyraclostrobin single agent and their mixtures on spore germination of the test strains were determined by the concave slide method.
[0027] 0.5 mL of the drug solution and 0.5 mL of the spore suspension were added dropwise to the concave slide, and 3 replicates were set for each concentration of the drug solution, with 3 concave slides as one replicate. The blank control was set with 0.1% Tween-80 aqueous solution. The concave slide holder was placed in a culture dish with a shallow layer of water and incubated at 28°C in the dark. When the blank control spore germination rate reached more than 80%, the germination of the treated spores was checked. Four fields of view were randomly observed for each concentration of the drug solution, and 200 spores were investigated to record the number of germinated spores and the total number of spores, and the relative inhibition rate of spore germination was calculated. The spore germination was considered to be greater than the short radius of the spore when the length of the spore germ tube was greater than the short radius of the spore.
[0028]
[0029]
[0030]
[0031] 4. Data analysis Linear regression was performed with the logarithmic value of the fungicide concentration as x and the relative inhibition rate of spore germination as y to calculate the toxicity regression equation and the toxicity EC 50 value of the fungicide to the target pathogen. The co-toxicity coefficient (CTC) was calculated using the Sun Yunpei method.
[0032]
[0033] In the above formula: ATI --the actual toxicity index of the mixed agent; S --the EC 50 of the standard fungicide, with units of mg / L; M --the EC 50 of the mixed agent, with units of mg / L.
[0034]
[0035] In the above formula: TTI --the theoretical toxicity index of the mixed agent; TI A --the toxicity index of agent A;P A --A agent percentage content in the mixture, unit: percentage (%); TI B --B agent toxicity index; P B --B agent percentage content in the mixture, unit: percentage (%).
[0036]
[0037] In the above formula: CTC --co-toxicity coefficient; ATI --measured toxicity index of the mixture; TTI --theoretical toxicity index of the mixture.
[0038] 5. Determination results According to the calculated co-toxicity coefficient (CTC), the type of action of the agent is evaluated: CTC≤80 is antagonistic action, 80<CTC<120 is additive action, and CTC≥120 is synergistic action, and the results are shown in Table 1.
[0039] Table 1 Toxicity determination results of ifenpropatrifluazole and pyrisoxazole compound on spore germination of Fusarium graminearum From Table 1, it can be seen that the toxicity EC values of ifenpropatrifluazole and pyrisoxazole single agents for inhibiting spore germination of Fusarium graminearum are 0.8255 mg / L and 0.6539 mg / L, respectively. When the two are compounded in a mass ratio of 1-9:9-1, the toxicity EC values of the compound combination for inhibiting spore germination of Fusarium graminearum are 0.2186-0.6499 mg / L, wherein when the two are compounded in a mass ratio of 3:1, the toxicity EC value is the smallest, and the inhibitory effect is the strongest. In addition, the co-toxicity coefficient of the compound combination is 123.7715-354.3807, which shows synergistic effect, and can improve the control effect on Fusarium graminearum. Especially when the mass ratio is 3:1, the co-toxicity coefficient is the largest, and the synergistic effect is the most significant. 50 50 50
[0040] In summary, the present application compounds ifenpropatrifluazole and pyrisoxazole into a pesticide active ingredient with fungicidal efficacy, which shows synergistic effect on Fusarium graminearum causing corn stem rot, can improve the control effect on corn stem rot, reduce the risk of drug resistance, and can provide support for the development of new high-efficiency compound fungicides for preventing and treating corn stem rot.
[0041] Example 2: Effect of different inert fillers on the thermal storage stability of pesticide fertilizer granules 2.1 Formulation of pesticide fertilizer granules 3 kg of triflumizole, 1 kg of pyraclostrobin, 3 kg of hydroxypropyl methyl cellulose (HPMC, Aldrin, item H108825), 1.5 kg of sodium dodecyl sulfate, inert fillers (see Tables 2 and 3) to 100 kg.
[0042] 2.2 Preparation process S1. The inert filler is crushed through a 200-mesh sieve and dried at 80°C to a moisture content of ≤5%; S2. The triflumizole and pyraclostrobin are mixed uniformly, then an aqueous solution of sodium dodecyl sulfate (1.5 kg of sodium dodecyl sulfate dissolved in 5 L of deionized water) is added, and the mixture is stirred and mixed uniformly. The inert filler is then added, mixed again, and a pesticide carrier premix is obtained; S3. The hydroxypropyl methyl cellulose is added to 12 L of deionized water at 40°C, and the fertilizer (0 in this example 2) and the pesticide carrier premix are added in turn after stirring. The mixture is stirred and mixed uniformly, and then extruded to form granules with a diameter of 2.0 ± 0.2 mm. The granules are dried at 45°C to a moisture content of ≤3%, and a fertilizer granule is obtained.
[0043] 2.3 Thermal storage stability Referring to "2.3 Other formulations" in "GB / T 1936-2003 Determination of thermal storage stability of pesticides", 20 g of the sample is placed in a glass bottle and spread into a smooth and uniform layer. The glass bottle is then placed in a constant-temperature oven at 54 ± 2°C. After 14 days, it is removed and placed in a desiccator to cool to room temperature. Within 12 hours, the contents of triflumizole and pyraclostrobin in the sample are separated and determined by high-performance liquid chromatography, and the decomposition rate is calculated. The results are shown in Tables 2 and 3.
[0044] Table 2 Effect of single inert filler on thermal storage stability of fertilizer granules As can be seen from Table 2, different inert fillers have different effects on the thermal storage stability of triflumizole and pyraclostrobin. Among them, bentonite has a more significant effect on the thermal storage stability of the active ingredients, while attapulgite has the smallest effect.
[0045] Table 3 Effect of different inert filler combinations on thermal storage stability of fertilizer granules As can be seen from Table 3, different inert filler combinations have different effects on the thermal storage stability of triflumizole and pyraclostrobin. Among them, the inert filler combination of attapulgite, diatomite and kaolin at a mass ratio of 1:1-3:1 can simultaneously reduce the thermal storage decomposition rate of triflumizole and pyraclostrobin, indicating that there is a synergistic effect among the three, which can further improve the thermal storage stability of the active ingredients.
[0046] Example 3: Effect of different fertilizers combined with inert fillers on the thermal storage stability of the pesticide-fertilizer granules 3.1 Pesticide-fertilizer granule formulation 3 kg of efinaconazole, 1 kg of pydiflumetofen, 42 kg of fertilizer (see Table 4), 3 kg of hydroxypropyl methyl cellulose, 1.5 kg of sodium dodecyl sulfate, and inert fillers to make up to 100 kg; wherein the inert fillers are mixed at a mass ratio of 1:3:1 of attapulgite, diatomite and kaolin.
[0047] 3.2 Preparation process The same as Example 2 “2.2 Preparation process”.
[0048] 3.3 Thermal storage stability The determination method is the same as Example 2 “2.3 Thermal storage stability”, and the results are shown in Table 4.
[0049] Table 4 Effect of different fertilizers on the thermal storage stability of pesticide-fertilizer granules As can be seen from Table 4, monoammonium phosphate, ammonium sulfate, potassium sulfate single fertilizer and the mixed fertilizer of the three at a mass ratio of 3:7:5 have less effect on the thermal storage stability of the effective components efinaconazole and pydiflumetofen. Considering the composition of fertilizer nutrients, the mixture of monoammonium phosphate, ammonium sulfate and potassium sulfate at a mass ratio of 3:7:5 is selected as the fertilizer component of the pesticide-fertilizer granules.
[0050] Example 4: Control effect of pesticide-fertilizer granules on corn stem rot 4.1 Pesticide-fertilizer granule formulation 3 kg of efinaconazole, 1 kg of pydiflumetofen, 42 kg of fertilizer (9 kg of monoammonium phosphate, 21 kg of ammonium sulfate and 15 kg of potassium sulfate), 3 kg of hydroxypropyl methyl cellulose, 1.5 kg of sodium dodecyl sulfate, and inert fillers to make up to 100 kg; wherein the inert fillers are mixed at a mass ratio of 1:3:1 of attapulgite, diatomite and kaolin.
[0051] 4.2 Preparation process The same as Example 2 “2.2 Preparation process”.
[0052] 4.3 Pot experiment Take field soil, dry in the shade, crush and pass through a 20-mesh sieve to obtain test field soil. Fill the test field soil into plastic pots to 3 / 4 of the volume, and irrigate the bottom of the pots to make the soil completely wet, then sow corn seeds (Guishan 162) in the plastic pots, 1 seed per pot. When the corn seedlings grow to the 6-leaf stage, use a disposable syringe to make a hole at the base of the corn seedling stem and inject 2.5 mL of 1.0 x 10 7A spore suspension of 104 spores / mL of F. graminearum (prepared in the same manner as in Example 1) was placed in a 25℃ light for 12h / 20℃ dark for 12h condition and cultured. After 2d, 2.5g of the granular pesticide fertilizer was hole-applied around each corn seedling, the test was set in 4 replicates, 5 corn seedlings for one replicate, and a treatment without any pesticide and fertilizer was set as a blank control. The stem disease healthy boundary was taken as a sample 30d after the pesticide fertilizer was applied, the control rate of the granular pesticide fertilizer on corn stem rot was calculated, and the results are shown in Table 5.
[0053]
[0054] Table 5 Prevention and treatment effect of the pesticide fertilizer of the present application on corn stem rot treatment average value of lesion area control rate chemical fertilizer treatment 6.24 mm 2 ]]> 79.15% blank control 29.93 mm 2 ]] -- As can be seen from Table 5, the granular pesticide fertilizer of the present application can effectively prevent and treat corn stem rot.
[0055] In summary, the pesticide active ingredient prepared by compounding ifen-trifluconazole and pyraclostrobin and the fertilizer are combined to form a functional granular pesticide fertilizer, which has both pesticide effect and fertilizer effect, improves the disease control effect, can reduce the amount of pesticide application, at the same time can supplement the nutrients required for the growth of corn plants, promotes plant growth and improves stress resistance, one-time application, gets double effect of pesticide and fertilizer, and has good application prospect.
[0056] The foregoing description of specific exemplary embodiments of the present application is intended to be illustrative only and is not intended to limit the present application to the precise forms described. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the present application. The particular exemplary embodiments were chosen and described in order to explain the principles of the present application and its practical application to thereby enable others skilled in the art to best utilize the present application and various embodiments with various modifications as are suited to the particular use contemplated. It is intended that the scope of the present application be defined by the claims and their equivalents.
Claims
1. An active ingredient in a pesticide, characterized in that, The active ingredient of the pesticide is a compound of isofentrifluconazole and fluopyram at a mass ratio of 1-9:9-1.
2. The pesticide active ingredient according to claim 1, characterized in that, The mass ratio of isofentrifluconazole to fluoxetine is 3:
1.
3. A granular fertilizer formulation, characterized in that, The pesticide granules comprise the active pesticide ingredient as described in claim 1.
4. The granulated fertilizer-pesticide according to claim 3, characterized in that, The fertilizer granules, by weight percentage, consist of the following components: 1-5% isofenfluconazole, 0.5-2% fluopyram, 15-45% fertilizer, 2-5% binder, 1-3% dispersant, and inert filler to 100%.
5. The granulated fertilizer-pesticide according to claim 4, characterized in that, The fertilizer granules, by weight percentage, consist of the following components: 3% isofenfluconazole, 1% fluopyram, 45% fertilizer, 3% binder, 1.5% dispersant, and inert filler to 100%.
6. The granulated fertilizer-pesticide according to claim 4, characterized in that, The fertilizer is a mixture of monoammonium phosphate, ammonium sulfate and potassium sulfate in a mass ratio of 3:7:
5.
7. The granulated fertilizer-pesticide according to claim 4, characterized in that, The adhesive is hydroxypropyl methylcellulose.
8. The granulated fertilizer-pesticide according to claim 4, characterized in that, The dispersant is sodium dodecyl sulfate.
9. The granulated fertilizer-pesticide according to claim 4, characterized in that, The inert filler is composed of attapulgite, diatomaceous earth and kaolin in a mass ratio of 1:3:1.