Sanguinarine sterilization composition containing macleaya cordata extract and application of sanguinarine sterilization composition

Abstract

The invention discloses a sanguinarine sterilization composition containing a macleaya cordata extract and application of the sanguinarine sterilization composition. The sanguinarine sterilization composition mainly comprises sanguinarine and pydiflumetofen. The two components do not have cross resistance, the two medicaments have obvious synergistic effect after being combined according to a certain ratio, and a common agricultural bactericide dosage form is prepared by adding a proper amount of agricultural bactericide auxiliaries and has higher control effect on fungal diseases of crops. Through combined use of the formula, the dosage can be reduced, the drug resistance of germs to a single agent can be delayed, ecological damage and environmental pollution are effectively reduced, and the yield and quality of crops can be improved; the composition can efficiently prevent and control crop sickle root rot, increases a disease prevention spectrum, and can prevent common crop diseases such as anthracnose, leaf spot, early blight, brown blotch and gray mold at the same time.

Description

Technical Field

[0001] This invention belongs to the field of agricultural fungicides, specifically relating to a fungicidal composition of sanguisorbin extracted from *Gnaphalium affine* and fluopyram. By adding appropriate adjuvants, it is made into an agricultural fungicide that can effectively control diseases caused by Fusarium wilt in crops. This composition increases the control efficacy and broadens the disease resistance spectrum, and can also prevent common fungal diseases such as anthracnose, leaf spot, brown spot, and gray mold in crops. Background Technology

[0002] Crop diseases seriously threaten crop yield and quality. There are many types of crop diseases, among which those caused by the genus *Fusarium* (*Fusarium*) are prevalent. Fusarium Fusarium graminearum (FJP) infection causing root and stem base diseases is one of the most persistent problems in plant growth. It is a class of pathogens that are widespread in crops, causing significant damage and severely impacting crop yield and quality; it is considered "cancer" in plants and is difficult to control. Fusarium graminearum is a widely distributed and diverse fungus, with approximately 500 species currently identified. The main pathogens include Fusarium graminearum (FJP). Fusarium graminearum Fusarium pseudograss () Fusarium pseudograminearum Fusarium solani () Fusarium solani Fusarium ( ) F . falciforme ), Fusarium neosporum ( F . neocosmosporiellum ), F . ipomoeae Fusarium moniliforme ( ) F . incarnatum ), Fusarium moniliforme ( F . proliferatum ), Fusarium pseudoverticum ( F . verticillioides ), Fusarium oxysporum ( F . fujikuroi Fusarium oxysporum ( ), F . oxysporum ) and yellow Fusarium ( F . culmorum Fusarium wilt can cause diseases in different parts of plants, such as peanut falcon rot, soybean falcon rot and sudden death syndrome, wheat scab, and corn ear rot. Fusarium can survive for a long time in the soil and on surviving plant remains, infecting plants after sowing under suitable conditions. Fusarium infects through the roots and has a long incubation period; once symptoms appear, it is difficult to save the plant, leading to wilting and death. Furthermore, when infecting plants, Fusarium produces large amounts of toxins, causing even more severe damage and indirectly toxicity to humans and livestock.

[0003] At present, the prevention and control of crop fusarium diseases mainly relies on chemical agents. However, long-term use of single agent and unreasonable application mode make the pathogenic bacteria easily develop resistance, leading to decreased efficacy, disease outbreak, and vicious cycle of increased use of chemicals, increased cost of chemicals, and aggravated environmental pollution, which is a major problem of chemical control. In addition, resistant strains of carbendazim may occur. Therefore, finding new control agents and reasonably compounding pesticides with different chemical structures and different mechanisms of action are effective measures to overcome the occurrence and development of drug resistance, which can enhance the control effect, reduce the amount of chemicals used per unit area, achieve the purpose of reducing chemicals and increasing efficiency, and provide solutions to the contradiction between agricultural efficiency and environmental pollution.

[0004] Pydiflumetofen is a phenyl-ethyl-pyrazole-aromatic amide succinate dehydrogenase inhibitor fungicide, which is a new type of fungicide developed by Syngenta in recent years. Its structural formula is (3-(difluoromethyl)-N-methoxy-1-methyl-N-[(RS)-1-methyl-2-(2,4,6-trichlorophenyl)ethyl]pyrazole-4-formamide), and its molecular formula is C 16 H 16 Cl3F2N3O2, boiling point 557.9℃ ± 50.0℃, density 1.44 ± 0.1 g / cm 3 Pydiflumetofen belongs to one of the succinate dehydrogenase inhibitors (SDHI) compounds, which interferes with the respiration of plant pathogens by inhibiting the activity of complex enzyme II. It has a different action spectrum from other succinate dehydrogenase inhibitor fungicides such as boscalid, fluopyram and benzovindiflucy, and there is no significant cross-resistance between these fungicides, which is presumably because it acts on different subunits of succinate dehydrogenase. Pydiflumetofen is highly effective and broad-spectrum, and is suitable for use in cereals, corn, soybeans, rapeseed, vegetables, fruit trees, special crops, lawns, ornamental plants, etc. It can control many diseases caused by pathogens such as fusarium ( Fusarium spp.), cerospora ( Cercospora spp.), botrytis ( Botrytis spp.), alternaria ( Alternaria spp.) and others, such as powdery mildew, leaf spot, brown spot, gray mold, scab, sclerotinia and others. However, there is no report on the control effect of pydiflumetofen on wilt disease.

[0005] Macleaya cordata is a perennial herb of the family Papaveraceae, and there are two species in the genus, i.e. M. cordata and M. microcarpa. M. cordata, also known as "Hao Tong Gang", is a traditional Chinese medicinal material rich in alkaloids and widely distributed in China. The main bioactive substances in M. cordata are isoquinoline alkaloids, such as sanguinarine, chelerythrine, protopine and allocryptopine, among which sanguinarine has the highest content and exhibits multiple biological activities, such as antimicrobial, insecticidal, antiviral, anti-inflammatory, antitumor, liver function improvement and immune enhancement. In agricultural production, sanguinarine, a natural alkaloid pesticide, has excellent insecticidal, fungicidal and miticidal activities and plays an important role in pollution-free prevention and control. SUMMARY

[0006] The present application provides a chemical synthetic compound and a plant-derived extract compound with remarkable control effect on fusarium diseases. Both compounds are efficient, green and environmentally friendly, and have different mechanisms of action without cross-resistance. When combined in a certain proportion, the combination has a remarkable synergistic effect, can efficiently prevent and control diseases caused by fusarium in crops, and can increase the disease resistance spectrum while increasing the control effect, and can also prevent common fungal diseases such as anthracnose, leaf spot, brown spot and gray mold.

[0007] The technical solution of the present application is as follows: A sanguinarine fungicide composition containing M. cordata extract and its application, wherein the fungicide is composed of sanguinarine extract from M. cordata and fluazinam.

[0008] The fungicide is characterized in that the mass ratio of sanguinarine to fluazinam is 20:1 to 1:20. When used for preventing and controlling fusarium root rot in crops, the mass ratio of sanguinarine to fluazinam is 5:1 to 1:1.

[0009] The effective components of sanguinarine and fluazinam account for 1% to 80% of the total mass of the fungicide composition. By adding an appropriate amount of agricultural fungicide adjuvant, a water suspension agent, a wettable powder, a microcapsule agent, a water dispersible granule or a suspended seed coating agent can be prepared to efficiently prevent and control fusarium root rot in major crops such as peanuts, soybeans and wheat, and simultaneously prevent common diseases such as anthracnose, leaf spot, early blight, brown spot and gray mold.

[0010] The significant effects and advantages of the present application are as follows: This invention combines sanguinarine, a plant-derived component extracted from a natural Chinese medicinal herb, with fluopyram, a highly efficient and green chemically synthesized pesticide. These two compounds possess different structural types and mechanisms of action. Sanguinarine primarily disrupts the cell walls and membranes of pathogens, while fluopyram targets and inhibits the activity of complex enzyme II to interfere with the respiration of plant pathogens. When combined in a specific ratio, the two compounds exhibit a significant synergistic effect. Sanguinarine accelerates the targeted action of fluopyram by disrupting the cell walls and membranes of pathogens. This composition increases both the efficacy and the spectrum of disease resistance. By adding highly effective adjuvants, it is processed into suspension concentrates, wettable powders, microcapsules, water-dispersible granules, or suspension seed coating agents that are easy to use in production. It has high biological activity, increases the disease prevention coverage, and has a good control effect on root rot and other common crop diseases that often occur in production. Moreover, after compounding and enhancing the effect, the total amount of medicine used per unit area is reduced, the number of times of application is reduced, the application cost is reduced, the development and growth of pathogen resistance is delayed, the service life of each component in the fungicide composition is extended, and it has good safety for crops, thus achieving the invention's purpose of being economical, efficient, and environmentally friendly.

[0011] The application of the bactericidal composition in the prevention and control of fungal diseases in crops.

[0012] A detailed analysis was conducted on the bactericidal properties of sanguinarine and fluopyram. First, the EC50 values ​​of the two single compounds (hereinafter referred to as the agents) were determined. 50 (Drug safety indicators), based on the EC50 of two single-drug formulations. 50 The combination ratio of the two agents is set according to the Wadley method, and the Wadley synergistic coefficient (SR) value is used as a reference.

[0013] The target organism in the experiment was Fusarium graminearum, which causes a common root rot disease in crops. Fusarium graminearum (But not limited to this). Sanguisorbin and fluopyram were prepared into plates using PDA solid medium, approximately 18 ml per plate. Mycelial blocks with a diameter of 5 mm were made from the edge of the pre-cultured colonies using a sterile punch, and transferred to different medium plates, incubated at 25°C. The experiment used a mycelial growth assay. Different treatment-containing mediums were prepared, and mycelial blocks with a diameter of 5 mm were made from the edge of the pre-cultured colonies using a sterile punch. These were transferred to medium plates with different drug concentrations, and the colony diameter was measured using the cross-sectional method. The growth inhibition rate was calculated using the following formula: Mycelial growth inhibition rate % = (1 - (diameter of treated colonies - 5) / (diameter of control colonies - 5)) × 100% The mycelium growth inhibition rate is converted into inhibition probability value (y), the concentration of the agent is converted into concentration logarithm (x), a toxicity regression straight line is drawn according to the concentration logarithm as the horizontal coordinate and the probability value as the vertical coordinate, the toxicity regression equation of sanguinarine and fluazinam single agent and the mixture thereof on the pathogen is obtained, and the EC 50 value and the correlation coefficient r value are calculated.

[0014] According to the Wadley method for evaluating the interaction of the mixture, the calculation formula is as follows: EC 50 (theoretical value) = (a+b) / (a / EC 50 a+ b / EC 50 b), SR = EC 50 (theoretical value) / EC 50 (actual value).

[0015] Wherein a and b are the content proportion of each component in the mixture, and the effect of the mixture is analyzed by the SR value. If SR≤0.5, the two agents mixed have antagonistic effect; if SR=0.5-1.5, the two agents mixed have additive effect; and if SR≥1.5, the two agents mixed have synergistic effect.

[0016] Table 1 Toxicity determination results of sanguinarine and fluazinam single agent and the mixture thereof on Fusarium graminearum

[0017] It can be seen from the above table that the bacteriostatic effects of the sanguinarine and fluazinam compositions with different proportions on Fusarium graminearum are different. The experimental results show that the sanguinarine and fluazinam have additive effect when the proportion is in the range of 15:1 to 1:10, and have synergistic effect when the proportion is in the range of 5:1 to 1:1.

[0018] The indoor combined action determination results show that the sanguinarine and fluazinam composition has good growth inhibition effect on Fusarium graminearum, and there is obvious synergistic effect between the two components; the two components can be mixed according to a certain proportion to reduce the amount of the agent, effectively reduce the ecological damage and environmental pollution, and improve the yield and quality of crops; the sanguinarine and fluazinam in the application both belong to low toxicity, and are safe to human, livestock, beneficial organisms and environment; there is no cross resistance between the sanguinarine and fluazinam, and the fungicides are also different, the mixed use of the two components can delay the drug resistance of the pathogen to the single agent, increase the disease prevention spectrum of crops, and achieve the purposes of combined prevention and drug reduction. DETAILED DESCRIPTION

[0019] The application will be further described below in combination with examples, but the content of the application is not limited to the following examples. Example 1

[0020] 18% sanguinarine • fluazinam water dispersible granule preparation method: according to the mass percentage.

[0021] Sanguinarine: 15%; Fluazinam: 3%; Sodium lignosulfonate: 6%; Polyvinyl alcohol: 4%; Ammonium sulfate: 8%; White carbon black: 10%; Kaolin: the balance; Preparation method: the above components are mixed in a mixer according to the formula proportion, and then air comminuted, and then a small amount of purified water is added for full stirring, extrusion granulation, and drying to obtain the water dispersible granule product. Example 2

[0022] 20% sanguinarine • fluazinam suspension concentrate preparation method: according to the mass percentage.

[0023] Sanguinarine: 10%; Fluazinam: 10%; Calcium lignosulfonate: 6%; Alkylphenol polyoxyethylene phosphate: 4%; Xanthan gum: 0.1%; Silicone defoaming agent: 0.3%; Water balance.

[0024] Preparation method: according to the formula, the above components are weighed according to the proportion, ball milled in a ball mill for 30 minutes, filtered, pumped into a dispersion tank for high-speed shearing for 3 minutes, and then fully ground in a sand mill, with the particle diameter of the solid component controlled within 2 microns. After grinding, stir evenly to obtain the suspension concentrate product. Example 3

[0025] 10% sanguinarine • fluazinam suspension seed coating preparation, according to the mass percentage.

[0026] Sanguinarine 5%, fluazinam 5%, xanthan gum: 3%, dispersant FS3000: 8%, didodecyl sodium sulfate: 4%, sodium lignosulfonate 3%, glycerol: 3%, bentonite: 5%, erie anhydrous fast T: 2%, film former: 3%, dye: 2%, water balance.

[0027] Preparation method: according to the formula, the above components (except film former and dye) are weighed according to the proportion, ball milled in a ball mill for 60 minutes, filtered, pumped into a dispersion tank for high-speed shearing for 3 minutes, and then fully ground in a sand mill, with the particle diameter of the solid component controlled within 1 μm. After grinding, stir evenly, and add the film former and the dye to obtain the suspension seed coating product.

[0028] Field control experiment of peanut root rot.

[0029] Peanut root rot control trials were conducted in 2023 and 2024. The 2024 trial data are summarized below. The experiments used Implementation 2, namely 20% sanguisorbin•fluoxadiazon suspension, 20% sanguisorbin suspension, 20% fluoxadiazon suspension, and 25% azoxystrobin suspension. The Trichosanthes kirilowii variety used in the trials was “Wanhua 4”. The trials were conducted at the Gangji Experimental Production Base of the Anhui Academy of Agricultural Sciences. The water usage was 45 kg per mu. Control measures were applied during the peanut seedling and early flowering stages, and a disease survey was conducted 45 days later (Table 2).

[0030] Table 2. Control effects of different compound formulations on peanut root rot

[0031] Field trials showed that the combination of sanguisorbin and fluopyram had a significant control effect on peanut root rot. The pesticide formulation formed by the synergistic combination exhibited better overall control of peanut root rot than any single formulation. Field surveys indicated that within the tested dosage range, crop growth was normal, and no phytotoxicity or abnormal phenomena were observed, demonstrating safety for peanuts. Field control experiment of soybean root rot.

[0032] The experiment used a suspension seed coating agent to coat soybean seeds. The experimental soybean variety was "Wan Dou 37". The experiment was conducted at the experimental base in Yongqiao District, Suzhou. The dosage of the agent was 1:500-1:1000 by weight for seed coating. The agent did not need to be diluted with water and was directly applied to the seeds. After the coated soybean seeds were naturally air-dried, they were sown. The germination rate, seedling height, root length, fresh weight, and control efficacy were investigated 20 days after sowing, and the control efficacy was investigated 40 days after sowing (Table 3).

[0033] Table 3. Effects of different seed dressing formulations on soybean growth and control of soybean sickle root rot

[0034] Field trials showed that a 10% sanguisorbin-fluoxazolamide suspension seed dressing significantly improved soybean emergence rate, but had no significant effect on soybean plant height, root length, or fresh weight. This result also indicates that the 10% sanguisorbin-fluoxazolamide suspension seed dressing can reduce the incidence of seed-borne diseases that prevent emergence, effectively reducing seed-transmitted diseases, and demonstrating significant field control efficacy. Field surveys showed that within the tested dosage range, crop growth was normal, and no phytotoxicity or abnormal phenomena were observed, indicating safety for soybeans.

[0035] Finally, it should be noted that the above enumeration is only several specific embodiments of the present application. Obviously, the present application is not limited to the above embodiments, and there are many variations. All variations that can be directly derived or inferred from the present application by those of ordinary skill in the art are considered to be within the scope of the present application.

Claims

1. A bactericidal composition containing sanguisorbin, an extract of *Euphorbia lathyris*, characterized in that: The bactericide comprises sanguisorbin extract and fluopyram, with a mass ratio of sanguisorbin to fluopyram at 20:1 to 1:

20.

2. The bactericidal composition according to claim 1, characterized in that: The mass ratio of sanguinarine to fluoxetine is 5:1 to 1:

1.

3. The bactericidal composition according to claim 1 or 2, characterized in that: It also contains agricultural auxiliary ingredients, with the active ingredient content ranging from 1% to 80% by weight.

4. The bactericidal composition according to claim 3, characterized in that: The bactericidal composition is formulated as a suspension, microemulsion, emulsifiable concentrate, wettable powder, microcapsule, water-dispersible granule, or suspension seed coating agent.

5. Use of the bactericidal composition according to any one of claims 1-4 for the prevention and control of crop diseases.

6. The use according to claim 5, characterized in that: The diseases mentioned are root rot, anthracnose, leaf spot, brown spot, and gray mold.

Images