Seaweed cyclic utilization process, seaweed cyclic utilization product and application of seaweed cyclic utilization product

Abstract

The invention provides a seaweed recycling process and a product and application thereof. The seaweed recycling process comprises the steps of preparation of seaweed nano-vesicles, preparation of seaweed polysaccharides, preparation of a seaweed / lactic acid bacteria fermentation product and preparation of seaweed fibers which are sequentially performed. Compared with single utilization of the seaweed, the method disclosed by the invention has the advantages that the economic value, ecological value and social value of the seaweed can be excavated to the maximum extent, and the seaweed nano-vesicles and the seaweed polysaccharides with high additional values can be extracted from the seaweed to be used as food additives and cosmetic raw materials; according to the method, seaweed is recycled, the purchase amount of new raw materials and the treatment cost of waste in the production process of an enterprise can be reduced, and meanwhile, the industrial chain is prolonged.

Description

Technical Field

[0001] This invention belongs to the field of algae extraction technology, specifically relating to a process for recycling seaweed, its products, and applications. Background Technology

[0002] Seaweed is a spore-bearing plant that lives in the ocean and is a major component of marine plants. It is rich in polysaccharides (such as fucoidan and fucoidan), amino acids, vitamins (B vitamins, C, and E), minerals (potassium, calcium, and magnesium), carotenoids, and other active ingredients. It can moisturize, fight oxidation, soothe and repair, and improve skin texture in multiple ways. Its natural origin makes it less irritating and suitable for people who are sensitive to chemical additives. Therefore, it has been increasingly favored by manufacturers and consumers.

[0003] CN104069059A discloses a seaweed extract, its preparation method, and its application. The preparation method includes: (1) crushing seaweed into coarse powder; (2) adding water to the coarse powder and then crushing and extracting it using a colloid mill; (3) adding diatomaceous earth and activated carbon to the extract, stirring and mixing; sedimentation; (4) centrifuging the supernatant; (5) adding seed crystals and freezing treatment; (6) thawing and filtering; and (7) reconstitution with water. The extract prepared by this invention is particularly suitable for use in cosmetics. Conventional seaweed preparation processes often use reagents such as ethanol and refining methods such as resins and membranes. However, organic reagent residues in resins and membranes, as well as ethanol and its entrained aldehyde impurities, can easily be introduced into the seaweed extract, becoming potential allergens. This invention avoids the use of these allergens and has high safety.

[0004] CN112979838BA discloses a seaweed polysaccharide extract, its application, and its preparation method. The seaweed polysaccharide extract is obtained from a combination of green and brown algae. Furthermore, the invention discloses a method for preparing the aforementioned seaweed polysaccharide extract, comprising the following steps: Step a: Weigh green and brown algae according to their dry weight ratio, mix and pulverize the mixture, sieve the pulverized material, and then perform ultrasonic extraction using a calcium chloride aqueous solution. Filter the extracted solution and collect the filtrate; Step b: Add polyamide and resin to the filtrate collected in Step a, stir for a period of time, filter, and collect the filtrate; Step c: Concentrate the filtrate collected in Step b to 1 / 5 to 1 / 8 of its original volume, filter, add ethanol to the filtrate, let it stand at 4-8°C for 4-6 hours, collect the solid, and dry it to obtain the final product. This seaweed polysaccharide extract exhibits significant synergistic moisturizing effects and can effectively promote skin barrier repair, making it suitable for the development of moisturizing and repairing products.

[0005] However, existing technologies mostly involve single extraction of seaweed, which cannot make multiple uses of seaweed and result in serious waste of resources. Therefore, there is an urgent need to provide a method that can maximize the utilization value of seaweed to meet application needs. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a seaweed recycling process, its products, and applications.

[0007] To achieve this objective, the present invention employs the following technical solution:

[0008] In a first aspect, the present invention provides a seaweed recycling process, which includes the sequential preparation of seaweed nanovesicles, preparation of seaweed polysaccharides, preparation of seaweed / lactic acid bacteria fermentation products, and preparation of seaweed fibers.

[0009] Preferably, the method for preparing the seaweed nanovesicles includes:

[0010] After pulverizing the seaweed, it was added to water, extracted, and then the solid and liquid were separated. The separated liquid was ultrafiltered, and the retentate was freeze-dried to obtain the seaweed nanovesicles. At the same time, the solids after solid-liquid separation were collected for the preparation of seaweed polysaccharides.

[0011] Preferably, the mass ratio of seaweed to water is 1:(20-30), for example, it can be 1:21, 1:23, 1:25, 1:27 or 1:29, etc.

[0012] Preferably, the extraction temperature is 20-40℃ (e.g., 22℃, 25℃, 28℃, 30℃, 32℃, 35℃ or 38℃, etc.), and the time is 30-60min (e.g., 35min, 40min, 45min, 50min or 55min, etc.).

[0013] Preferably, the ultrafiltration membrane used for ultrafiltration has a molecular weight cutoff of 5-20 kDa (e.g., 6 kDa, 8 kDa, 10 kDa, 12 kDa, 14 kDa, 16 kDa, or 18 kDa, etc.).

[0014] Preferably, the freeze-drying conditions are as follows:

[0015] Pre-freeze at -60℃ to -50℃ for 20-30 minutes; raise the temperature once to -35℃ to -15℃ and hold for 1-2 hours; raise the temperature a second time to -5℃ to -10℃ and hold for 1-2 hours.

[0016] Preferably, the method for preparing the seaweed polysaccharide includes:

[0017] The solids collected during the preparation of seaweed nanovesicles were added to water, heated and extracted, then separated into solid and liquid, the separated liquid was collected and concentrated to obtain seaweed polysaccharide, and the solids after solid-liquid separation were collected for the preparation of seaweed / lactic acid bacteria extract.

[0018] Preferably, the mass ratio of the fixative to water is 1:(30-50), for example, it can be 1:32, 1:35, 1:38, 1:40, 1:42, 1:45 or 1:48, etc.

[0019] Preferably, the temperature rise to 95-110℃ can be, for example, 96℃, 98℃, 100℃, 102℃, 104℃, 106℃ or 108℃.

[0020] Preferably, the extraction time is 1-2 hours, for example, 1.1 hours, 1.3 hours, 1.5 hours, 1.7 hours or 1.9 hours.

[0021] Preferably, the concentration to 10-25% of the volume of the separated liquid can be, for example, 11%, 13%, 15%, 17%, 19%, 21%, 23%, or 24%.

[0022] Preferably, the method for preparing the seaweed / lactic acid bacteria fermentation product includes:

[0023] The solid obtained during the preparation of seaweed polysaccharide is sterilized and added to a liquid culture medium. Then, lactic acid bacteria are inoculated into the liquid culture medium for fermentation. After solid-liquid separation, the separated liquid is sterilized to obtain the seaweed / lactic acid bacteria fermentation product. At the same time, the solid after solid-liquid separation is collected for the preparation of seaweed fiber.

[0024] Preferably, the liquid culture medium is MRS liquid culture medium. Preferably, the sterilization method includes pasteurization.

[0025] Preferably, the pasteurization temperature is 70-80℃ (e.g., 71℃, 73℃, 75℃, 77℃ or 79℃, etc.), and the time is 20-30min (e.g., 21min, 23min, 25min, 27min or 29min, etc.).

[0026] Preferably, the lactic acid bacteria include Lactobacillus plantarum or Lactobacillus rhamnosus.

[0027] Preferably, the inoculation amount is 10 7 -10 8 CFU / mL.

[0028] Preferably, the fermentation temperature is 35-40℃ (e.g., 36℃, 37℃, 38℃ or 39℃, etc.), and the time is 46-52h (e.g., 47h, 48h, 49h, 50h or 51h, etc.).

[0029] Preferably, the sterilization process includes filtering the separation liquid and collecting the filtrate.

[0030] Preferably, the pore size of the filter membrane during filtration is 0.2-0.25 μm, for example, it can be 0.21 μm, 0.22 μm, 0.23 μm or 0.24 μm, etc.

[0031] Preferably, the method for preparing seaweed fiber includes bleaching and drying the solid obtained during the preparation of seaweed / lactic acid bacteria fermentation products to obtain the seaweed fiber.

[0032] In a second aspect, the present invention provides an algae nanovesicle, an algae polysaccharide, an algae / lactic acid bacteria fermentation product, or an algae fiber, wherein the algae nanovesicle, algae polysaccharide, algae / lactic acid bacteria fermentation product, or algae fiber is prepared by the algae recycling process described in the first aspect.

[0033] Thirdly, this invention provides the application of seaweed nanovesicles, seaweed polysaccharides, and seaweed / lactic acid bacteria fermentation products as described in the second aspect in the preparation of cosmetics.

[0034] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) Compared with the single utilization of seaweed, the method of the present invention can maximize the economic, ecological and social value of seaweed. High-value-added seaweed nanovesicles and seaweed polysaccharides can be extracted from seaweed for use as food additives and cosmetic raw materials. Then, seaweed / lactic acid bacteria fermentation products are prepared through fermentation. Finally, the algae residue can be further processed into seaweed fiber. The method of the present invention recycles seaweed, which can reduce the "new raw material purchase volume" and "waste treatment cost" in the production process of enterprises. At the same time, it extends the industrial chain and allows enterprises to shift from "profit from a single product" to "profit from multiple categories".

[0037] (2) The product prepared by this invention reduces production costs while still having excellent moisturizing effect. The seaweed polysaccharide still has excellent antioxidant, antibacterial, soothing, antioxidant, oil-controlling and scalp redness-relieving effects. The seaweed / lactic acid bacteria fermentation product still has excellent effects in improving combability, strengthening hair quality and improving shine. When used in cosmetics, it can greatly improve the above-mentioned properties of the product. The seaweed fiber prepared has moisture-absorbing and breathable, antibacterial and antimicrobial effects, and can be better used in home textiles, clothing and other fields. Detailed Implementation

[0038] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention.

[0039] The raw materials used in the embodiments and comparative examples of this invention can be used as long as they are purchased from authorized distributors.

[0040] Example 1

[0041] This embodiment provides a seaweed recycling process, which includes the preparation of seaweed nanovesicles, the preparation of seaweed polysaccharides, the preparation of seaweed / lactic acid bacteria fermentation products, and the preparation of seaweed fibers. Specific steps include:

[0042] (1) Preparation of seaweed nanovesicles: After crushing seaweed, add it to water (the mass ratio of seaweed to water is 1:25), extract at 30℃ for 50 min, and then separate the solid and liquid. Filter the separated liquid using an ultrafiltration membrane with a molecular weight cutoff of 10 kDa. Freeze-dry the retentate (pre-freezing temperature -55℃, pre-freezing time 25 min; first heating to -25℃, holding time 1.5 h; second heating to 0℃, holding time 2 h) to obtain the seaweed nanovesicles. At the same time, collect the solids after solid-liquid separation for the preparation of seaweed polysaccharides.

[0043] (2) Preparation of seaweed polysaccharide: Add the solid collected in step (1) to water (the mass ratio of solid to water is 1:40), heat to 105℃ and extract for 1.5h, then separate the solid and liquid, collect the separated liquid, concentrate to 25% of the original volume to obtain seaweed polysaccharide, and collect the solid after solid-liquid separation for the preparation of seaweed / lactic acid bacteria extract;

[0044] (3) Preparation of seaweed / lactic acid bacteria fermentation products:

[0045] The solid obtained during the preparation of seaweed polysaccharide was pasteurized at 75°C for 25 min and then added to liquid culture medium. Lactobacillus rhamnosus was then inoculated (inoculation amount: 5 x 10⁻⁶). 7 The seaweed / lactic acid bacteria fermentation product was obtained by adding CFU / mL to liquid culture medium and fermenting at 38°C for 48 hours. Then, solid-liquid separation was performed, and the separated liquid was sterilized by passing it through a 0.22μm filter membrane. The filtrate was collected as the seaweed / lactic acid bacteria fermentation product.

[0046] (4) Preparation of seaweed fiber: The solid obtained during the preparation of seaweed / lactic acid bacteria fermentation products is bleached and dried to obtain the seaweed fiber.

[0047] In this embodiment, 500g of seaweed was used to prepare 0.82mg of seaweed nanovesicles (containing 3.12*10^6 vesicles). 11 The method of this invention yields 184.8g of seaweed polysaccharide, 128.3g of seaweed / lactic acid bacteria fermentation product, and 136.2g of seaweed fiber. Compared with single preparation methods, the method of this invention has a higher utilization rate of raw materials.

[0048] Example 2

[0049] This embodiment provides a seaweed recycling process, which includes the preparation of seaweed nanovesicles, the preparation of seaweed polysaccharides, the preparation of seaweed / lactic acid bacteria fermentation products, and the preparation of seaweed fibers. Specific steps include:

[0050] (1) Preparation of seaweed nanovesicles: After crushing seaweed, add it to water (the mass ratio of seaweed to water is 1:20), extract at 40℃ for 40 min, and then separate the solid and liquid. Filter the separated liquid using an ultrafiltration membrane with a molecular weight cutoff of 15 kDa. Freeze-dry the retentate (pre-freezing temperature -50℃, pre-freezing time 30 min; first heating to -15℃, holding time 2 h; second heating to 5℃, holding time 1 h) to obtain the seaweed nanovesicles. At the same time, collect the solids after solid-liquid separation for the preparation of seaweed polysaccharides.

[0051] (2) Preparation of seaweed polysaccharide: Add the solid collected in step (1) into water (the mass ratio of solid to water is 1:50), heat to 95℃ and extract for 2 hours, then separate the solid and liquid, collect the separated liquid, concentrate to 15% of the original volume to obtain seaweed polysaccharide, and collect the solid after solid-liquid separation for the preparation of seaweed / lactic acid bacteria extract.

[0052] (3) Preparation of seaweed / lactic acid bacteria fermentation products:

[0053] The solid obtained during the preparation of seaweed polysaccharide was pasteurized at 70°C for 30 min and then added to the liquid culture medium. Lactobacillus plantarum was then inoculated (inoculation amount: 1*10⁻⁶). 7 The seaweed / lactic acid bacteria fermentation product was obtained by adding CFU / mL to liquid culture medium and fermenting at 35°C for 52 hours. Then, solid-liquid separation was performed, and the separated liquid was sterilized by passing it through a 0.22μm filter membrane. The filtrate was collected as the seaweed / lactic acid bacteria fermentation product.

[0054] (4) Preparation of seaweed fiber: The solid obtained during the preparation of seaweed / lactic acid bacteria fermentation products is bleached and dried to obtain the seaweed fiber.

[0055] In this embodiment, 500g of seaweed was used to prepare 0.79mg of seaweed nanovesicles (containing 2.65*10^6 vesicles). 11 (g / particles); seaweed polysaccharide 186.7g; seaweed / lactic acid bacteria fermentation product 123.9g; seaweed fiber 140.7g.

[0056] Example 3

[0057] This embodiment provides a seaweed recycling process, which includes the preparation of seaweed nanovesicles, the preparation of seaweed polysaccharides, the preparation of seaweed / lactic acid bacteria fermentation products, and the preparation of seaweed fibers. Specific steps include:

[0058] (1) Preparation of seaweed nanovesicles: After crushing seaweed, add it to water (the mass ratio of seaweed to water is 1:30), extract at 20℃ for 60 min, and then separate the solid and liquid. Filter the separated liquid using an ultrafiltration membrane with a molecular weight cutoff of 5 kDa. Freeze-dry the retentate (pre-freezing temperature -60℃, pre-freezing time 20 min; first heating to -35℃, holding time 1 h; second heating to -5℃, holding time 1.5 h) to obtain the seaweed nanovesicles. At the same time, collect the solids after solid-liquid separation for the preparation of seaweed polysaccharides.

[0059] (2) Preparation of seaweed polysaccharide: Add the solid collected in step (1) to water (the mass ratio of solid to water is 1:30), heat to 110℃ and extract for 1 hour, then separate the solid and liquid, collect the separated liquid, concentrate to 20% of the original volume to obtain seaweed polysaccharide, and collect the solid after solid-liquid separation for the preparation of seaweed / lactic acid bacteria extract;

[0060] (3) Preparation of seaweed / lactic acid bacteria fermentation products:

[0061] The solid obtained during the preparation of seaweed polysaccharide was pasteurized at 80℃ for 20 min and then added to liquid culture medium. Lactobacillus rhamnosus was then inoculated (inoculation amount: 1*10⁻⁶). 8 The seaweed / lactic acid bacteria fermentation product was obtained by adding CFU / mL to liquid culture medium and fermenting at 40℃ for 46h. Then, solid-liquid separation was performed, and the separated liquid was sterilized by passing it through a 0.22μm filter membrane. The filtrate was collected as the seaweed / lactic acid bacteria fermentation product.

[0062] (4) Preparation of seaweed fiber: The solid obtained during the preparation of seaweed / lactic acid bacteria fermentation products is bleached and dried to obtain the seaweed fiber.

[0063] In this embodiment, 500g of seaweed was used to prepare 0.88mg of seaweed nanovesicles (containing 1.98*10^6 vesicles). 11 (particles / g); seaweed polysaccharide 188.2g; seaweed / lactic acid bacteria fermentation product 122.7g; seaweed fiber 133.7g.

[0064] Test Example 1: Moisturizing Performance Test of Algae Nanovesicles

[0065] The seaweed nanovesicles obtained in Examples 1-3 were added to the serum (the serum composition was 2% seaweed nanovesicles, 10% butylene glycol, 0.5% carbomer, 0.1% disodium EDTA, 1.5% polyglycerol-6 distearate, 2% jojoba esters, 0.5% arginine, 1% p-hydroxyacetophenone, and 3% squalane, with the remainder made up to 100% with water) at a dosage of 2%. The serum without added seaweed nanovesicles served as a control. Forty healthy female volunteers, aged 20-40 years, without serious systemic diseases, immunodeficiency, or autoimmune diseases, and without cosmetic allergies, acute inflammation, use of hormones or immunosuppressants, participation in other clinical trials, or application of topical preparations, were randomly divided into four groups of 10 participants each. The prepared sample was applied to the right arm of each participant. Skin moisture was measured using a Corneometer at 0 min, 30 min, and 60 min. The CM825 test was used to measure skin hydration and assess the skin hydration before and after using the product. The specific test results are shown in Table 1 below.

[0066] Table 1

[0067]

[0068] As shown in Table 1, compared with the control example, the essence, by adding seaweed nanovesicles, can have a good moisturizing effect. The skin moisture content can reach more than 38% after 30 minutes and is still more than 41% after 60 minutes, indicating that the seaweed nanovesicles prepared by the present invention have excellent moisturizing effect.

[0069] Test Example 2: Antibacterial Performance Test of Seaweed Polysaccharides

[0070] (1) Test sample: seaweed polysaccharides prepared in Examples 1-3;

[0071] (2) Test basis: Refer to the American Clinical Laboratory Standards Committee's drug susceptibility testing procedure (action time is 5 min);

[0072] Main instruments: constant temperature incubator, ultra-clean workbench, hole punch, pipette, small forceps, sterile plates;

[0073] Test strain: Malassezia furfur.

[0074] The results are shown in Table 2.

[0075] Table 2

[0076]

[0077] As shown in Table 2, the seaweed polysaccharide involved in this invention has an inhibition zone of over 40 mm against Malassezia furfur, indicating that it has a good inhibitory effect on Malassezia furfur.

[0078] Test Example 3: Determination of the minimum inhibitory concentration of seaweed polysaccharides against Propionibacterium acnes:

[0079] Test subjects: The seaweed polysaccharides provided in Examples 1-3 were added to shampoo (the composition of the shampoo is 15% sodium laureth sulfate, 2% lauryl glucoside, 1% cocamidopropyl betaine, 3% sodium lauroyl hydroxyethyl sulfonate, 0.5% carbomer, 0.5% EDTA-2Na, 0.4% fragrance, 2% glycerin, 5% seaweed polysaccharides, with the remainder made up to 100% by water) as test samples. Shampoos without added seaweed polysaccharides were used as control examples.

[0080] Prepare a 100 mg / mL sample of the shampoo from Example 1. Prepare ten test tubes (the first tube is empty, and the other nine contain 5 mL of distilled water). Add 5 mL of sample to the first test tube; then add 5 mL of sample to the second test tube and mix well; then add 5 mL of diluent to the third test tube, and so on, until the tenth test tube, at which point 5 mL is removed and discarded. Add 0.1 mL of Propionibacterium acnes bacterial suspension (100,000 CFU / mL) to each of the ten test tubes, mix well, and incubate at 37°C for 24 h. Observe the clear test tubes and determine the minimum inhibitory concentration (MIC).

[0081] The procedures for Examples 2-3 and the control examples were the same as above, and the results are shown in Table 3:

[0082] Table 3

[0083]

[0084] As shown in Table 3, compared with shampoo without added seaweed polysaccharides, the seaweed polysaccharides prepared in this invention have a significant inhibitory effect on Propionibacterium acnes.

[0085] Test Example 4: Seaweed Polysaccharide Test for Balancing Scalp Oil

[0086] Test subjects: The seaweed polysaccharides provided in Examples 1-3 were added to shampoo (the composition of the shampoo is 15% sodium lauryl ether sulfate, 2% lauryl glucoside, 1% cocamidopropyl betaine, 3% sodium lauroyl hydroxyethyl sulfonate, 0.5% carbomer, 0.5% EDTA-2Na, 0.4% fragrance, 2% glycerin, 5% seaweed polysaccharides, with the remainder made up to 100% by water) as test samples. Shampoos without added seaweed polysaccharides were used as control examples.

[0087] The effectiveness of shampoos in balancing sebum and reducing scalp oiliness is evaluated by measuring the amount of sebum on the scalp surface.

[0088] The testing method is as follows:

[0089] Twenty-eight volunteers with oily hair were recruited and randomly divided into four groups. Each group used the aforementioned shampoo for four consecutive weeks, washing their hair every three days. The laboratory temperature was controlled at 20℃ and the humidity at 40%. Participants refrained from strenuous exercise and significant sweating for two hours prior to the sebum measurement and sat quietly in the laboratory for 20 minutes. Scalp sebum levels were measured 48 hours after shampooing in the fourth week. Test sites were located approximately 3 cm to the sides of the scalp midline and 3 cm longitudinally from the hairline. During testing, the hair was parted to expose the scalp, and a SEBUMETER SM810 measuring instrument was used to press on the measurement site for 30 seconds before reading the value. The highest reading was recorded from both sides. The test results are shown in Table 4 below.

[0090] Table 4

[0091]

[0092] As shown in Table 4, compared with shampoo without added seaweed polysaccharides, the seaweed polysaccharides prepared in this invention can effectively balance scalp oil and reduce scalp oil production.

[0093] Test Example 5: Dry and Wet Combing Performance Test of Seaweed / Lactic Acid Bacteria Fermentation Products

[0094] Test samples: The seaweed / lactic acid bacteria fermentation products provided in Examples 1-3 were added to shampoo (the composition of the shampoo is 15% sodium laureth sulfate, 2% lauryl glucoside, 1% cocamidopropyl betaine, 3% sodium lauroyl hydroxyethyl sulfonate, 0.5% carbomer, 0.5% EDTA-2Na, 0.4% fragrance, 2% glycerin, 2% seaweed / lactic acid bacteria fermentation products, with the remainder made up to 100% by water) as test samples. Shampoos without added seaweed / lactic acid bacteria fermentation products were used as control examples.

[0095] (1) Pretreatment and simulated use: Take 4 groups of hair strands and clean them with a 10% SLES (sodium dodecyl ether sulfate) aqueous solution. The specific steps are as follows:

[0096] a) Soak the hair bundle in a 10% SLES (sodium dodecyl ether sulfate) aqueous solution for 5 minutes;

[0097] b) Rinse the hair strands with clean water for 2 minutes;

[0098] Repeat this process twice to complete the basic cleaning of the hair strands, and then use a hair dryer to dry the hair strands;

[0099] (2) The hair strands were randomly divided into test groups, and the samples were used in a simulated manner:

[0100] 1) Rinse the hair strands with clean water for 1 minute;

[0101] 2) Apply 2g of test sample to a damp section of hair and massage thoroughly for 1 minute;

[0102] 3) Rinse the hair strands with clean water for 2 minutes;

[0103] This process is repeated twice to complete the sample simulation.

[0104] (3) Wet combing performance test: The test group with the moisture controlled was tested for wet combing performance using Baosheng TA.XTC-20 texture analyzer. The maximum load was tested. The smaller the maximum load, the better the combing performance of the tested hair bundle.

[0105] (4) Dry combing performance test: The test group that has completed the wet combing performance test is dried with a hair dryer and the dry combing performance test is performed using the Baosheng TA.XTC-20 texture analyzer. The maximum load is tested. The smaller the maximum load, the better the combing performance of the tested hair strand.

[0106] The specific test results are shown in Table 5 below:

[0107] Table 5

[0108]

[0109] As shown in Table 4, compared with the dry and wet combing performance of the shampoo without the addition of seaweed / lactic acid bacteria fermentation products, the shampoo with the addition of the seaweed / lactic acid bacteria fermentation products prepared in this invention has better dry and wet combing performance.

[0110] The applicant declares that this invention illustrates a seaweed recycling process, its products, and applications through the above embodiments. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, additions of auxiliary components, and selection of specific methods all fall within the protection and disclosure scope of this invention.

[0111] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0112] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A process for recycling seaweed, characterized in that, The seaweed recycling process includes the sequential preparation of seaweed nanovesicles, seaweed polysaccharides, seaweed / lactic acid bacteria fermentation products, and seaweed fibers.

2. The seaweed recycling process as described in claim 1, characterized in that, The method for preparing the seaweed nanovesicles includes: After pulverizing the seaweed, it was added to water, extracted, and then the solid and liquid were separated. The separated liquid was ultrafiltered, and the retentate was freeze-dried to obtain the seaweed nanovesicles. At the same time, the solids after solid-liquid separation were collected for the preparation of seaweed polysaccharides.

3. The seaweed recycling process as described in claim 2, characterized in that, The mass ratio of seaweed to water is 1:(20-30); Preferably, the extraction temperature is 20-40℃ and the extraction time is 30-60 min; Preferably, the ultrafiltration membrane used for ultrafiltration has a molecular weight cutoff of 5-20 kDa; Preferably, the freeze-drying conditions are as follows: Pre-freeze at -60℃ to -50℃ for 20-30 minutes; raise the temperature once to -35℃ to -15℃ and hold for 1-2 hours; raise the temperature a second time to -5℃ to -10℃ and hold for 1-2 hours.

4. The seaweed recycling process as described in any one of claims 2-3, characterized in that, The preparation method of the seaweed polysaccharide includes: The solids collected during the preparation of seaweed nanovesicles were added to water, heated and extracted, then separated into solid and liquid, the separated liquid was collected and concentrated to obtain seaweed polysaccharide, and the solids after solid-liquid separation were collected for the preparation of seaweed / lactic acid bacteria extract.

5. The seaweed recycling process as described in claim 4, characterized in that, The mass ratio of the fixed object to water is 1:(30-50); Preferably, the temperature is raised to 95-110℃; Preferably, the extraction time is 1-2 hours.

6. The seaweed recycling process as described in any one of claims 4-5, characterized in that, The method for preparing the seaweed / lactic acid bacteria fermentation product includes: The solid obtained during the preparation of seaweed polysaccharide is sterilized and added to a liquid culture medium. Then, lactic acid bacteria are inoculated into the liquid culture medium for fermentation. After solid-liquid separation, the separated liquid is sterilized to obtain the seaweed / lactic acid bacteria fermentation product. At the same time, the solid after solid-liquid separation is collected for the preparation of seaweed fiber.

7. The seaweed recycling process as described in claim 6, characterized in that, The sterilization method includes pasteurization; Preferably, the pasteurization temperature is 70-80℃ and the time is 20-30 minutes; Preferably, the lactic acid bacteria include Lactobacillus plantarum or Lactobacillus rhamnosus; Preferably, the inoculation amount is 10 7 -10 8 CFU / mL; Preferably, the fermentation temperature is 35-40℃ and the time is 46-52 hours; Preferably, the sterilization process includes filtering the separation liquid and collecting the filtrate. Preferably, the pore size of the filter membrane during filtration is 0.2-0.25 μm.

8. The seaweed recycling process as described in any one of claims 6-7, characterized in that, The method for preparing seaweed fiber includes bleaching and drying the solid obtained during the preparation of seaweed / lactic acid bacteria fermentation products to obtain the seaweed fiber.

9. A type of seaweed nanovesicle, seaweed polysaccharide, seaweed / lactic acid bacteria fermentation product, or seaweed fiber, characterized in that, The seaweed nanovesicles, seaweed polysaccharides, seaweed / lactic acid bacteria fermentation products, or seaweed fibers are prepared using the seaweed recycling process described in any one of claims 1-8.

10. The application of the seaweed nanovesicles, seaweed polysaccharides, or seaweed / lactic acid bacteria fermentation products as described in claim 9 in the preparation of cosmetics.

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