Filling scheme for multi-reagent filling liquid of micro-fluidic chip

Abstract

The invention discloses a filling system and a filling method for multi-reagent filling liquid of a micro-fluidic chip, and belongs to the technical field of micro-fluidic chip filling. The problems that an existing micro-fluidic chip filling system cannot meet the requirement for automation and a chip pre-embedded reagent is not suitable for a universal scene are solved. The filling system provided by the invention comprises a reagent storage unit, a multi-channel injection unit and a liquid separation unit, the reagent storage unit is connected with the multi-channel injection unit through a liquid inlet pipeline, and the multi-channel injection unit is connected with the liquid separation unit through a liquid outlet pipeline; the micro-fluidic chip is provided with a filling port, and the liquid separation unit is connected with the filling port of the micro-fluidic chip through a liquid conveying pipeline. According to the invention, the reagent can be automatically injected into the micro-fluidic chip according to the reaction dosage and the set time sequence, and the device can be suitable for universal scenes.

Description

Technical Field

[0001] This invention relates to the field of microfluidic chip technology, and in particular to a dispensing system and method for multi-reagent dispensing solutions in microfluidic chips. Background Technology

[0002] Microfluidic chips are microstructures with microchannels that can manipulate microfluidics from nanoliters to picoliters within a chip-scale environment. This feature allows for the transfer of biological reaction operations—previously performed in specialized laboratories by professionals using specialized equipment—to within the chip itself. This effectively reduces equipment size, reagent consumption, and improves equipment portability and the environmental adaptability of biochemical reactions.

[0003] Generally, microfluidic chips need to be used in conjunction with reagents. Before or during operation, reagents need to be injected into the chip in sequence and according to volume requirements so that the reagents can be manipulated and reacted within the chip.

[0004] Currently, the process and functional design of microfluidic chips have become relatively mature. However, the injection of reagents into the chip, especially the injection of a wide variety of reagents with inconsistent volumes, is a major problem in the current application of microfluidic chips.

[0005] There are three main types of reagent dispensing for microfluidic chips:

[0006] The first method is manual dispensing using a pipette. This method is universal and easy to implement, but its disadvantage is that it cannot meet the requirements of full automation.

[0007] The second method involves pre-embedding reagents into the microfluidic chip during its fabrication. During use, the reagent capsules are squeezed pneumatically or electrically, allowing the reagents to enter the chip sequentially and in the correct quantity to participate in the reaction process. The disadvantages are that this type of chip is too expensive to manufacture and its application is highly specific to particular scenarios, making it unsuitable for general-purpose use.

[0008] The third method involves establishing a universal droplet workstation using a pipette arm system. This system combines the movement of a multi-functional pipette arm along the XYZ axes with a high-precision pipette tip, utilizing the principle of an air injection pump to achieve automated dispensing. This method can simultaneously dispense multiple reagents without cross-contamination; however, its disadvantage is that the workbench occupies a large area, which is not conducive to the miniaturization of the equipment. Summary of the Invention

[0009] In view of the above analysis, the present invention aims to provide a dispensing system and method for multi-reagent dispensing solutions for microfluidic chips, in order to solve at least one of the following problems:

[0010] (1) Solve the problem that manual reagent dispensing cannot meet the needs of automation;

[0011] (2) Solve the problem that chip-embedded reagents are not applicable to general scenarios;

[0012] (3) Solve the problem that the size of the pipette droplet workstation is too large and cannot be utilized by miniaturization design.

[0013] The objective of this invention is mainly achieved through the following technical solutions:

[0014] On the one hand, the present invention provides a dispensing system for multi-reagent dispensing solutions for microfluidic chips, including a reagent storage unit, a multi-channel injection unit, and a dispensing unit;

[0015] The reagent storage unit is connected to the multi-channel injection unit via an inlet pipe, and the multi-channel injection unit is connected to the dispensing unit via an outlet pipe. The microfluidic chip is equipped with a filling port, and the dispensing unit is connected to the filling port of the microfluidic chip via an infusion pipe.

[0016] In one possible design, the reagent storage unit includes a reagent temperature-controlled chamber;

[0017] The reagent temperature control chamber includes a main chamber body, a refrigeration execution module, and a drive and control module; the refrigeration execution module is located inside the main chamber body; the refrigeration execution module is connected to the drive and control module via signals.

[0018] In one possible design, the refrigeration actuation module includes a reagent placement tank and a temperature sensor;

[0019] The temperature sensor is located on the reagent placement tank and is connected to the drive and control module.

[0020] In one possible design, the cooling execution module also includes insulation components;

[0021] The heat insulation component is located around the reagent placement tank and is used to insulate and keep the reagent placement tank warm.

[0022] In one possible design, the cooling execution module also includes a thermoelectric cooler and a heat sink; both the thermoelectric cooler and the heat sink are electrically connected to the drive and control module.

[0023] The thermoelectric cooler is located at the bottom of the reagent placement tank; the heat dissipation component is located at the bottom of the thermoelectric cooler.

[0024] In one possible design, the cooling execution module also includes components that accelerate heat dissipation;

[0025] The heat dissipation acceleration component is located below the heat dissipation component and is electrically connected to the drive and control module.

[0026] In one possible design, the reagent storage unit also includes a room-temperature reagent storage compartment;

[0027] The room temperature reagent storage compartment contains multiple room temperature reagent storage bottles.

[0028] In one possible design, the multichannel injection unit includes a first multichannel injection pump and a second multichannel injection pump;

[0029] The first multi-channel syringe pump is connected to the corresponding reagent temperature control chamber, and the second multi-channel syringe pump is connected to the room temperature reagent storage chamber.

[0030] In one possible design, the dispensing unit includes a rotary dispensing valve;

[0031] Both the first and second multichannel syringe pumps are connected to a rotary dispensing valve; the rotary dispensing valve is connected to the infusion port of the microfluidic chip via an infusion pipeline.

[0032] On the other hand, the present invention also provides a method for dispensing multi-reagent solution for microfluidic chips, using the above-mentioned dispensing system for multi-reagent solution of microfluidic chips for dispensing reagents; the dispensing method includes the following steps:

[0033] Step 1: Based on the actual amount of reagents used in the reaction process and the temperature requirements for storing low-temperature reagents, set up a multi-channel injection unit and a dispensing unit.

[0034] Step 2: Connect the reagent storage unit, multi-channel injection unit, dispensing unit, and microfluidic chip in sequence;

[0035] Step 3: Automatically add reagents to the microfluidic chip according to the reaction amount and predetermined timing.

[0036] Compared with the prior art, the present invention can achieve at least one of the following beneficial effects:

[0037] (1) The present invention utilizes a first multi-channel injection pump and a second multi-channel injection pump with a self-contained power source and a rotary valve for switching valves to realize the automatic dispensing of various reagents in microfluidic chips, and has the characteristics of high precision, automation and miniaturization.

[0038] (2) By setting up a reagent temperature control chamber, this invention provides low-temperature storage conditions for low-temperature reagents; by setting up a room-temperature reagent storage chamber, it provides room-temperature storage conditions for room-temperature reagents; this invention provides solutions for reagent storage at different temperatures. In addition, this invention solves the problem of reagent sedimentation by setting up a peristaltic pump and using the peristaltic pump to blow air into the reagent.

[0039] (3) By setting a rotary dispensing valve, the present invention forms a plug-in structure between the rotary dispensing valve and the pipeline connected thereto, which makes it convenient for users to replace reagents and pipeline consumables during use.

[0040] In this invention, the above-described technical solutions can be combined with each other to achieve more preferred combinations. Other features and advantages of this invention will be set forth in the following description, and some advantages may become apparent from the description or be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained through the embodiments described and the accompanying drawings. Attached Figure Description

[0041] The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Throughout the drawings, the same reference numerals denote the same parts.

[0042] Figure 1 This is a schematic diagram of the composition of the dispensing system of the present invention;

[0043] Figure 2 This is a schematic diagram of the composition of the reagent temperature control chamber of the present invention;

[0044] Figure 3 This is a schematic diagram of a rotary separator valve.

[0045] Figure label:

[0046] 1-Refrigeration execution module; 2-Drive and control module; 3-Temperature sensor; 4-Insulation plate; 5-Reagent placement tank; 6-Semiconductor cooling chip; 7-Heat sink; 8-Powered cooling fan; 9-Peristaltic pump; 10-First multi-channel syringe pump; 11-Second multi-channel syringe pump; 12-Rotary dispensing valve; 13-Microfluidic chip. Detailed Implementation

[0047] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which constitute a part of the present invention and are used together with the embodiments of the present invention to illustrate the principles of the present invention, but are not intended to limit the scope of the present invention.

[0048] On the one hand, the present invention provides a dispensing system for multi-reagent dispensing solutions of a microfluidic chip 13, such as... Figure 1 and Figure 2 As shown, the dispensing system includes a reagent storage unit, a multi-channel injection unit, and a dispensing unit; the reagent storage unit is connected to the multi-channel injection unit through an inlet pipe, and the multi-channel injection unit is connected to the dispensing unit through an outlet pipe; the microfluidic chip 13 is provided with a dispensing port, and the dispensing unit is connected to the dispensing port of the microfluidic chip 13 through an infusion pipe.

[0049] Specifically, the multi-channel injection unit of the present invention can inject the reagents provided by the reagent storage unit into the microfluidic chip 13 through the dispensing unit according to the dosage and predetermined timing, so as to realize the automatic dispensing of multiple reagents through multiple channels, expand the types and quantities of reagents, and meet the requirements of high precision, multiple types and high stability of fluid sample introduction and transmission.

[0050] To provide a constant low-temperature environment for reagents requiring low-temperature storage, the reagent storage unit of this invention includes a reagent temperature-controlled chamber, such as... Figure 2 As shown, the reagent temperature control chamber includes a chamber body, a refrigeration execution module 1, and a drive and control module 2; the refrigeration execution module 1 is located inside the chamber body; the refrigeration execution module 1 is signal-connected to the drive and control module 2.

[0051] Specifically, the drive and control module 2 includes a controller, a power supply module, and a drive circuit. The controller is used to receive the temperature signal sent by the refrigeration execution module 1 and control the working state of each component of the refrigeration execution module 1 according to the preset temperature range. The power supply module is used to provide a stable power supply for the entire filling system. The drive circuit can drive the components in the refrigeration execution module 1 to perform refrigeration work according to the instructions of the controller.

[0052] Compared with existing technologies, the present invention can provide a constant low-temperature environment for reagents that require low-temperature storage by setting up a reagent temperature control chamber.

[0053] To better provide a constant low-temperature environment for reagents requiring low-temperature storage, the refrigeration execution module 1 of this invention includes a reagent placement tank 5 and a temperature sensor 3, such as... Figure 2 As shown, temperature sensor 3 is installed on reagent placement tank 5, and temperature sensor 3 is connected to drive and control module 2.

[0054] Specifically, the reagent placement tank 5 is a uniformly heated aluminum block tank, and the temperature sensor 3 is located at the bottom of the uniformly heated aluminum block tank. The temperature sensor 3 is connected to the controller. The temperature sensor 3 can monitor the ambient temperature inside the reagent placement tank 5 in real time and convert the temperature signal into an electrical signal and transmit it to the controller.

[0055] Compared with existing technologies, this invention, by installing a temperature sensor 3 at the bottom of the reagent placement tank 5, can sense the temperature inside the reagent placement tank 5 in real time, convert it into a temperature signal, and finally transmit the temperature signal to the controller as a reference for temperature adjustment. The temperature sensor 3 has an accuracy of 0.03℃ and a millisecond-level response speed, enabling precise monitoring of the ambient temperature inside the reagent placement tank 5.

[0056] Since the reagent placement tank 5 needs to maintain a certain temperature, the cooling execution module 1 also includes a heat preservation component; such as... Figure 2As shown, the heat insulation component is located around the reagent placement tank 5, and is used to insulate and keep the reagent placement tank 5 warm.

[0057] Specifically, the insulation component of this invention is an insulation board 4, which is disposed around the reagent placement tank 5. The insulation board 4 is made of rigid foam plastic board made of polystyrene resin, which has moisture-proof and waterproof properties. It can effectively reduce the thickness of the outer protective structure of the reagent placement tank 5, thereby increasing the indoor usable area. As a heat insulation layer, the insulation board 4 can reduce the exchange of heat between the inside and outside and maintain the temperature stability inside the reagent placement tank 5.

[0058] Compared with the prior art, the present invention reduces heat transfer and loss of the reagent placement tank 5 by setting heat insulation components around the reagent placement tank 5, thereby achieving the functions of heat insulation and heat preservation.

[0059] To cool the reagent placement tank 5, the cooling execution module 1 of the present invention further includes a semiconductor cooling chip 6 and a heat dissipation component; such as Figure 2 As shown, both the thermoelectric cooler 6 and the heat dissipation component are electrically connected to the drive and control module 2; the thermoelectric cooler 6 is located at the bottom of the reagent placement tank 5; the heat dissipation component is located at the bottom of the thermoelectric cooler 6; the thermoelectric cooler 6 includes a hot end and a cold end, the cold end of the thermoelectric cooler 6 is adjacent to and in contact with the reagent placement tank 5, and the heat dissipation component is located on the hot end of the thermoelectric cooler 6, and the heat dissipation component is used to dissipate heat from the hot end of the thermoelectric cooler 6.

[0060] Specifically, both the thermoelectric cooler 6 and the heat dissipation component are connected to the controller. The top of the thermoelectric cooler 6 contacts the reagent placement tank 5, meaning the thermoelectric cooler 6 is located at the bottom of the reagent placement tank 5, and the bottom surface of the thermoelectric cooler 6 is connected to the heat dissipation component. The thermoelectric cooler 6 is connected to the controller and the power module, and operates according to the controller's instructions. The thermoelectric cooler 6 utilizes the Peltier effect of semiconductor materials to achieve cooling. When direct current passes through a thermocouple formed by two different semiconductor materials connected in series, heat is absorbed and released at the two ends of the thermocouple, thereby achieving the purpose of cooling. This method using the thermoelectric cooler 6 eliminates the need for a refrigerant and has no sliding parts, resulting in high reliability.

[0061] It should be noted that the heat dissipation component of this invention is a heat sink 7, which is installed on the hot end of the thermoelectric cooler 6 and is in close contact with the hot end of the thermoelectric cooler 6. The bottom of the heat sink 7 has a rectangular serrated structure. Designing the structure of the heat sink 7 as a rectangular serrated structure can increase its surface area to transfer heat from the heat source (the hot end of the thermoelectric cooler 6) to the air, thereby achieving the purpose of heat dissipation. The heat sink 7 can effectively help the reagent placement tank 5 maintain a suitable working temperature and prevent damage due to overheating.

[0062] Alternatively, the heat sink 7 can consist of multiple thin, connected fins arranged side-by-side to form a dense and tortuous channel structure. This design increases the surface area of ​​the heat sink 7 in contact with the air, thereby improving heat exchange efficiency. Each heat sink 7 has many tiny fins, which further increase the surface area for heat exchange, facilitating heat transfer between the air and the semiconductor cooling chip 6.

[0063] It should be noted that the heat sink 7 of the present invention is made of aluminum alloy, which has good thermal conductivity and processing properties, and can quickly absorb or dissipate heat. In summary, the heat sink 7 of the present invention can effectively absorb, conduct, and dissipate heat, ensuring that the reagent placement tank 5 for heat-generating electronic components can be maintained within a suitable operating temperature range.

[0064] Compared with existing technologies, this invention, by incorporating a semiconductor cooling chip 6, can cool the reagent placement tank 5, ensuring a constant low-temperature environment for reagents requiring low-temperature storage. The addition of a heat sink 7 increases the heat dissipation area, accelerating the dissipation of heat generated by the semiconductor cooling chip 6.

[0065] To further dissipate heat from the hot end of the semiconductor cooling chip 6, the cooling execution module 1 of the present invention also includes a heat dissipation acceleration component; the heat dissipation acceleration component is located below the heat dissipation component and is electrically connected to the drive and control module 2.

[0066] Specifically, the heat dissipation acceleration component of the present invention is a forced cooling fan 8, which is installed at the bottom of the heat sink 7. The forced cooling fan 8 is connected to the power supply and controller and operates according to the controller's instructions. The forced cooling fan 8 is used to provide forced convection and accelerate heat dissipation, that is, the forced cooling fan 8 is used to enhance the heat dissipation effect of the heat sink 7 or the thermoelectric cooler 6, ensuring that the cooling execution module 1 can effectively control the temperature even when operating under high load.

[0067] Compared with the prior art, the present invention further improves the heat dissipation efficiency of the heat sink 7 by setting a powerful cooling fan 8 to perform forced convection.

[0068] It should be noted that the reagent storage unit of the present invention also includes a room temperature reagent storage chamber; the room temperature reagent storage chamber is provided with a plurality of room temperature reagent storage bottles.

[0069] In summary, the temperature sensor 3 of this invention is connected to the controller, the controller is connected to the power module and the drive circuit, and both the thermoelectric cooler 6 and the heat sink 7 are connected to the controller through the drive circuit. The power module is used to supply power to the entire dispensing system. During operation, the temperature sensor 3 can monitor the ambient temperature in real time and transmit the signal to the controller. The controller makes a judgment based on the preset temperature range and the received temperature signal. If the temperature exceeds the preset range, the controller adjusts the working state of the thermoelectric cooler 6 and the forced cooling fan 8 through the drive circuit. The thermoelectric cooler 6 starts or stops cooling, and the forced cooling fan 8 starts or stops as needed to accelerate the heat dissipation effect of the heat sink 7. During this period, the insulation plate 4 continuously provides heat insulation to ensure the temperature of the controlled object or space is stable. Through this closed-loop control system, the temperature of the reagent placement tank 5 is precisely controlled.

[0070] It should be noted that the reagent storage bottles or room temperature reagent storage bottles in the reagent placement tank 5 of the present invention are made of PC (polycarbonate), PP (polypropylene) and PET (polyethylene terephthalate). These materials have no physical or chemical impact on the properties and quality of reagents and solutions, have good temperature tolerance, high pressure resistance, and good sealing and sterilization properties.

[0071] To achieve multi-channel injection of reagents, the multi-channel injection unit of the present invention includes a first multi-channel injection pump 10 and a second multi-channel injection pump 11; the first multi-channel injection pump 10 is connected to the reagent temperature control chamber, and the second multi-channel injection pump 11 is connected to the room temperature reagent storage chamber.

[0072] Specifically, the low-temperature reagent storage bottles in the reagent temperature control chamber are all connected to the first multi-channel injection pump 10 through different liquid inlet pipes, and the multiple room-temperature reagent storage bottles in the room-temperature reagent storage chamber are all connected to the second multi-channel injection pump 11 through different liquid inlet pipes. Each liquid inlet pipe is equipped with a shut-off valve, so that different reagents can be injected into the microfluidic chip 13.

[0073] Compared with the prior art, the present invention provides a power source for reagent dispensing by setting a first multi-channel injection pump 10 and a second multi-channel injection pump 11, and injects the reagents used into the microfluidic chip 13 in sequence according to the dosage and predetermined timing, and finally achieves selective dispensing of different low temperature reagents and room temperature reagents.

[0074] The liquid dispensing unit of the present invention includes a rotary dispensing valve 12; as shown in the figure. Figure 3 As shown, both the first multi-channel syringe pump 10 and the second multi-channel syringe pump 11 are connected to the rotary dispensing valve 12; the rotary dispensing valve 12 is connected to the infusion port of the microfluidic chip 13 through the infusion pipeline.

[0075] Specifically, the rotary dispensing valve 12 of the present invention adopts an equal-diameter double-channel pagoda head, which has good chemical corrosion resistance, can be sterilized with ethylene oxide or autoclave, and when used with a flexible hose, has good wear resistance, good pressure resistance, good airtightness, good temperature resistance, and is not easy to age, thus ensuring a stable liquid supply.

[0076] Compared with existing technologies, this invention utilizes a constant-diameter dual-channel pagoda head to link the first multi-channel syringe pump 10 and the second multi-channel syringe pump 11, expanding the types and quantities of reagents in the system. Furthermore, this invention solves the problem of chip-embedded reagents being unsuitable for general-purpose scenarios by switching reagents through the constant-diameter dual-channel pagoda head; the configuration and integration of the first multi-channel syringe pump 10, the second multi-channel syringe pump 11, and the constant-diameter dual-channel pagoda head solves the problem that manual reagent dispensing cannot meet automation requirements; and it achieves a compact reagent injection system, solving the problem of excessively large pipette arm droplet workstations that cannot be miniaturized.

[0077] It should be noted that the pipes and fittings involved in this invention are all made of PC (polycarbonate), PP (polypropylene) and PET (polyethylene terephthalate), and the pipe diameter is between φ0.5 and φ3 mm.

[0078] It should be noted that the dispensing system for the multi-reagent dispensing solution of the microfluidic chip 13 of the present invention also includes a peristaltic pump 9, which is connected to both the low-temperature reagent storage bottle and the room-temperature reagent storage bottle. Furthermore, the peristaltic pump 9 is also connected to the microfluidic chip 13. The functions of the peristaltic pump 9 include: firstly, as a pneumatic device, it regularly aerators the easily precipitated low-temperature or room-temperature reagent storage bottles according to a programmed time interval, ensuring the homogeneity of the solution during use; secondly, as a droplet extraction device, it provides the power source for the outflow of waste liquid from the microfluidic chip 13.

[0079] It should be emphasized that the dispensing system for multi-reagent dispensing solution of microfluidic chip 13 of the present invention also includes a host computer control unit; the rotary dispensing valve 12 and the first multi-channel injection pump 10 and the second multi-channel injection pump 11 are all connected to the host computer control unit for control.

[0080] On the other hand, the present invention also provides a method for dispensing a multi-reagent dispensing solution for a microfluidic chip 13, which uses the above-described dispensing system for the multi-reagent dispensing solution of the microfluidic chip 13 and includes the following process:

[0081] Step 1: Based on the actual amount of reagents used in the reaction process and the temperature requirements for storing low-temperature reagents, set up a multi-channel injection unit and a dispensing unit.

[0082] In step 1 above, based on the actual amount of reagents used in the reaction process and the temperature environment requirements for storing low-temperature reagents, the opening and closing sequence of the pipeline valves controlled by the first multi-channel injection pump 10 and the second multi-channel injection pump 11 and the corresponding injection volume are programmed using the host computer control unit.

[0083] Step 2: Connect the reagent storage unit, the multi-channel injection unit, the dispensing unit, and the microfluidic chip 13 in sequence;

[0084] In step 2 above, the reagent storage unit is connected to the multi-channel injection unit through the liquid inlet pipe, and the multi-channel injection unit is connected to the dispensing unit through the liquid outlet pipe; the microfluidic chip 13 is provided with a filling port, and the dispensing unit is connected to the filling port of the microfluidic chip 13 through the liquid inlet pipe; the dispensing unit (rotary dispensing valve 12) only changes the selection of the pipe, and does not change the amount and speed of reagent droplet dispensing;

[0085] Step 3: Automatically add reagents to the microfluidic chip 13 according to the reaction amount and predetermined timing.

[0086] In step 3 above, the host computer control unit controls the first multi-channel injection pump 10, the second multi-channel injection pump 11, and the rotary dispensing valve 12 to automatically add reagents to the microfluidic chip 13 according to the reaction amount and predetermined sequence.

[0087] Compared with the prior art, firstly, the present invention utilizes a first multi-channel syringe pump 10 and a second multi-channel syringe pump 11 with self-contained power source and a dispensing rotary valve to switch valves, which can realize the automatic dispensing of various reagents in the microfluidic chip 13, and has the characteristics of high precision, automation and miniaturization.

[0088] Secondly, this invention provides low-temperature storage conditions for low-temperature reagents by setting up a reagent temperature control chamber, and provides room-temperature storage conditions for room-temperature reagents by setting up a room-temperature reagent storage chamber; this invention provides solutions for reagent storage at different temperatures. Furthermore, this invention solves the problem of reagent sedimentation by setting up a peristaltic pump 9 and using the peristaltic pump 9 to blow air into the reagent.

[0089] Furthermore, the present invention provides a rotary dispensing valve 12, which forms a plug-in structure with the pipeline connected to it, allowing users to replace reagents and pipeline consumables during use.

[0090] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A dispensing system for multi-reagent dispensing solutions in microfluidic chips, characterized in that, Includes a reagent storage unit, a multi-channel injection unit, and a dispensing unit; The reagent storage unit is connected to the multi-channel injection unit via an inlet pipe, and the multi-channel injection unit is connected to the dispensing unit via an outlet pipe; the microfluidic chip is provided with a filling port, and the dispensing unit is connected to the filling port of the microfluidic chip via an infusion pipe.

2. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 1, characterized in that, The reagent storage unit includes a reagent temperature-controlled compartment; The reagent temperature control chamber includes a chamber body, a refrigeration execution module, and a drive and control module; the refrigeration execution module is located inside the chamber body; the refrigeration execution module is signal-connected to the drive and control module.

3. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 2, characterized in that, The refrigeration execution module includes a reagent placement tank and a temperature sensor; The temperature sensor is located on the reagent placement tank and is connected to the drive and control module via signal connection.

4. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 3, characterized in that, The refrigeration execution module also includes a heat insulation component; The heat-insulating component is located around the reagent placement tank, and the heat-insulating component is used to insulate and keep the reagent placement tank warm.

5. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 4, characterized in that, The cooling execution module further includes a semiconductor cooling chip and a heat dissipation component; both the semiconductor cooling chip and the heat dissipation component are electrically connected to the drive and control module. The semiconductor cooling chip is located at the bottom of the reagent placement tank; the heat dissipation component is located at the bottom of the semiconductor cooling chip.

6. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 5, characterized in that, The cooling execution module also includes a component that accelerates heat dissipation; The accelerated heat dissipation component is located below the heat dissipation component, and the accelerated heat dissipation component is electrically connected to the drive and control module.

7. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 6, characterized in that, The reagent storage unit also includes a room temperature reagent storage compartment; The room temperature reagent storage chamber is equipped with multiple room temperature reagent storage bottles.

8. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 7, characterized in that, The multi-channel injection unit includes a first multi-channel injection pump and a second multi-channel injection pump; The first multi-channel injection pump is connected to the corresponding reagent temperature control chamber, and the second multi-channel injection pump is connected to the room temperature reagent storage chamber.

9. The dispensing system for multi-reagent dispensing solutions of microfluidic chips according to claim 8, characterized in that, The liquid dispensing unit includes a rotary dispensing valve; Both the first multi-channel injection pump and the second multi-channel injection pump are connected to the rotary dispensing valve; the rotary dispensing valve is connected to the infusion port of the microfluidic chip through an infusion pipeline.

10. A method for adding multi-reagent dispensing solution to a microfluidic chip, characterized in that, The reagent is dispensed using the dispensing system for multi-reagent dispensing solutions of microfluidic chips as described in any one of claims 1 to 9; the dispensing method includes the following steps: Step 1: Based on the actual amount of reagents used in the reaction process and the temperature requirements for storing low-temperature reagents, set up a multi-channel injection unit and a dispensing unit. Step 2: Connect the reagent storage unit, multi-channel injection unit, dispensing unit, and microfluidic chip in sequence; Step 3: Automatically add reagents to the microfluidic chip according to the reaction amount and predetermined timing.

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