High-temperature-resistant planting method for anoectochilus formosanus

Abstract

The invention belongs to the technical field of plant planting, and particularly relates to a high-temperature-resistant anoectochilus formosanus planting method which sequentially comprises the following steps of semi-closed planter arrangement, sterilization, matrix preparation, specific nutrient solution addition, tissue culture seedling field planting and greenhouse facility arrangement. By means of the method, the anoectochilus formosanus can be safely summered for four months at the external extreme high temperature of 38 DEG C, the extreme high temperature in a greenhouse of 43 DEG C and the external monthly average high temperature of 31 DEG C, and the anoectochilus formosanus grows well. The planted anoectochilus formosanus is not damaged by plant diseases and insect pests, the anoectochilus formosanus planting process is easy to manage, the method has the advantages of being easy and convenient to operate, low in management cost, high in survival rate and the like, the problem that the precious medicinal plant anoectochilus formosanus can safely live in summer in low-altitude and high-temperature areas is solved, and technical support is provided for enlarging anoectochilus formosanus planting industrialization.

Description

Technical Field

[0001] This invention belongs to the field of plant cultivation technology, specifically relating to a method for cultivating Anoectochilus roxburghii to withstand high temperatures. Background Technology

[0002] *Anoectochilus roxburghii*, a variegated orchid belonging to the genus *Anoectochilus* in the family Orchidaceae, is a terrestrial herb. Rich in polysaccharides, steroidal compounds, organic acids, terpenoids, alkaloids, volatile oils, flavonoids, and glycosides, *Anoectochilus roxburghii* possesses anti-inflammatory, anti-tumor, hepatoprotective, antioxidant, and anti-HBV biological activities. It is primarily used to treat type II diabetes, hyperuricemia in the elderly, acute and chronic hepatitis, suppurative osteomyelitis, and oral ulcers. Its application is safe and has broad prospects for development and utilization.

[0003] Golden Thread Orchid is a shade-loving plant with a low light saturation point. It prefers a cool, low-light or diffused light environment, with an optimal growth temperature of 18-25℃. The cultivation of Golden Thread Orchid is easily limited by climatic conditions, especially in hot and humid environments where diseases such as stem rot become severe, making industrial-scale cultivation difficult. While greenhouse cooling methods can be implemented, they are costly and may also lead to disease outbreaks.

[0004] Golden Thread Orchid (Anoectochilus roxburghii) is intolerant of high temperatures and easily suffers from heat stress. In Fujian, it is generally planted around March. By June, when the temperature reaches around 28℃, the survival rate of Golden Thread Orchid gradually decreases. According to the cultivation substrate disclosed in patent publication number CN110915610A, the survival rate of Golden Thread Orchid is about 99% after two months of cultivation. After four months of cultivation (June, when the temperature reaches around 28℃), the survival rate drops to 80%. After five months of cultivation, the survival rate drops to 70%, and after six months of cultivation, the survival rate drops to below 50%. Therefore, how to ensure the healthy growth of Golden Thread Orchid and maintain a high survival rate during the high-temperature season after June, thereby increasing the fresh / dry weight and the content of medicinal active substances, is a technical problem that needs to be solved.

[0005] In view of this, researching and optimizing planting methods in order to obtain a planting method that can safely survive the high temperatures of summer and grow well is an urgent need for the industrialized cultivation of Anoectochilus roxburghii. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a method for cultivating Anoectochilus roxburghii that is resistant to high temperatures, which is not only easy to operate, but also has a more ideal planting effect.

[0007] The technical solution adopted by this invention to solve its technical problem is:

[0008] A method for cultivating Anoectochilus roxburghii (Golden Thread Orchid) to withstand high temperatures, wherein the method, by constructing a semi-enclosed microenvironment and combining it with specific cultivation management measures, enables Anoectochilus roxburghii to grow safely in summer conditions with an average monthly temperature of 30°C to 35°C for 3 to 5 months, with a survival rate of not less than 95%; the method includes the following steps in sequence:

[0009] Step 1: Planter Preparation and Sterilization: A semi-enclosed planter is provided, which consists of an openable and closable ventilated cover, a seedling tray with a permeable mesh at the bottom, and a water storage tray supporting the seedling tray. The ventilated cover has a height of 140-160 mm, a length of 530-570 mm, and a width of 290-310 mm. It has at least two sets of ventilation holes, each set containing 3-5 ventilation holes with a diameter of 5-8 mm, and is equipped with an openable and closable cover. The seedling tray has a depth of 55-65 mm. Before use, all components of the planter are cleaned and disinfected.

[0010] Step 2: Preparation of cultivation substrate: The cultivation substrate is prepared by mixing peat moss and perlite with a specific nutrient solution in a volume ratio of (5-7):(2-4):(0.8-1.2) and stirring evenly; wherein the pH value of the peat moss is 5.0-7.0 and the particle size is 1-10 mm.

[0011] Step 3: Substrate disinfection and tray filling: Fill the seedling trays with the cultivation substrate prepared in Step 2 to a depth of 5.0-6.5cm, and disinfect the substrate surface by spraying a carbendazim solution with a concentration of 4.0-12.0 mg / L.

[0012] Step 4: Pretreatment and transplanting of tissue culture seedlings: Select robust rooted tissue culture seedlings of Anoectochilus roxburghii with a plant height of 4.5-7.0 cm, a root length of 1.5-5.0 cm, and 2-4 roots. After hardening and acclimatization, remove them from the culture container, wash off the culture medium attached to the roots, and soak them in 50% carbendazim solution diluted 400-600 times for 2-4 minutes for disinfection, or in 30% hymexazol solution diluted 800-1200 times for 1-2 minutes for disinfection. After drying, plant them in the seedling trays that have been filled with disinfected substrate in Step 3.

[0013] Step 5: Planter sealing and water management: Place the seedling tray with golden thread lotus seedlings in the water storage tray, pour water into the water storage tray to a depth of 3-5 cm, and add 2-4 g of slow-release fertilizer with an effective period of 150-210 days. Then cover it with the breathable and sealed cover and close the vent holes to form an initial sealed and moisturizing environment.

[0014] Step Six: Greenhouse Facilities and Environmental Control: Place the semi-enclosed planters prepared in Step Five inside the greenhouse facilities, with a spacing of 8-12 cm between planters; the greenhouse is equipped with a shade net with a shading rate of 65% to 85%, and the light intensity is controlled at 2500-5500 Lux. An automatic sprinkler system is set up to spray for 8-12 minutes during the high-temperature period from 11:00 to 14:00 every day to cool down; throughout the high-temperature season, the growth of Anoectochilus roxburghii mainly relies on the semi-enclosed microenvironment and water storage tray for water and fertilizer supply, without the need for additional watering and soil fertilization.

[0015] Furthermore, the seedling tray mentioned in step one is a rectangular plug tray with a length of 530-550 mm and a width of 270-290 mm, and has 28-36 planting holes.

[0016] Furthermore, the water storage tray mentioned in step one has a height of 60-70 mm, a length of 530-550 mm, and a width of 280-290 mm.

[0017] Furthermore, in step one, there is a 1-3mm gap between the bottom of the closed cover and the upper surface of the ventilated cover for water and air permeability. Furthermore, the closed cover is not opened during the planting period.

[0018] Furthermore, the specific nutrient solution described in step two contains the following components per liter: 8-12 ml of amino oligosaccharide, 18-22 g of Flower Treasure No. 2, 8-12 g of Bacillus subtilis powder, and 4-10 g of trace element fertilizer. Furthermore, each liter of the nutrient solution also contains 0.045-0.065 g of arginine. Furthermore, in each liter of nutrient solution, the micronutrient fertilizer comprises 1.5-2.5 g of nitrogen (N), 0.8-1.2 g of phosphorus pentoxide (P2O5), 1.5-2.5 g of potassium oxide (K2O), 0.02-0.04 g of magnesium oxide (MgO), 0.015-0.025 g of boron (B), 0.002-0.004 g of zinc (Zn), 0.045-0.065 g of arginine, 0.005-0.030 g of molybdenum (Mo), 0.050-0.090 g of manganese (Mn), 0.3-0.5 g of iron (Fe), 0.8-1.2 g of sodium hydroxide (SO3), and 0.01-0.03 g of copper (Cu).

[0019] Furthermore, in step four, the hardening conditions are: ambient temperature 20-30℃, shading rate 65-85%, and light intensity controlled at 2500-5500 lux; the hardening process includes closed culture for 8-18 days, semi-open culture for 2-4 days, and fully open culture for 2-6 days.

[0020] Furthermore, the slow-release fertilizer brand mentioned in step five is the Canadian "Flower Color" slow-release fertilizer.

[0021] This invention also provides the application of the above-mentioned high-temperature resistant cultivation method of Anoectochilus roxburghii in the industrial cultivation of Anoectochilus roxburghii in low-altitude, high-temperature areas.

[0022] Furthermore, the cultivation equipment used for seedling transplanting is a seedling box with a breathable, sealed cover. The seedling box with a breathable, sealed cover includes: 1. A breathable, sealed cover: transparent plastic, 150mm high × 550mm long × 300mm wide, with two sets of ventilation holes, each set having 4 holes with a diameter of 6mm, and each set having a closable cover; 2. A rectangular plastic seedling tray with a mesh bottom: 60mm high × 540mm long × 280mm wide, with 32 holes per seedling tray, planting 32 seedlings; 3. A water storage tray: 65mm high × 540mm long × 285mm wide.

[0023] Furthermore, the cultivation substrate used for transplanting the seedlings is peat moss: perlite: nutrient solution = 6:3:1 (volume ratio), mixed thoroughly. The peat moss is from the Finnish brand Kejira, with particles of 2-8mm and a pH of 5.5-6.5.

[0024] Further, fill the mixed cultivation substrate into rectangular plastic seedling trays, cover them tightly, and spray with fungicide carbendazim at 5.0-10.0 mg / L.

[0025] Next, gently remove the acclimatized and hardened *Anoectochilus roxburghii* tissue culture seedlings from the bottle, and wash the culture medium off the roots, avoiding any damage or breakage. Soak the seedlings in a 500-fold diluted 50% carbendazim solution for 3 minutes for disinfection, then air dry them in a cool, shaded place before planting them into rectangular plastic seedling trays. Place a water-filled tray in the seedling tray, adding 3g of Canadian "Flower Color" slow-release fertilizer (effective for 180 days) to each tray. Cover with a breathable, sealed lid, then close the lid completely.

[0026] The beneficial effects of this invention are as follows:

[0027] The method of this invention allows *Anoectochilus roxburghii* to safely survive the summer for four consecutive months under extreme temperatures of 43°C in greenhouses and an average monthly temperature of 31°C outside, while maintaining vigorous growth. Furthermore, the cultivated *Anoectochilus roxburghii* is free from pests and diseases, and the cultivation process is simple to manage, offering advantages such as ease of operation, low management costs, and high survival rates. This solves the problem of ensuring the safe summer survival of this valuable medicinal plant in low-altitude, high-temperature areas, providing technical support for the industrialization of *Anoectochilus roxburghii* cultivation. In other words, the method of this invention overcomes the technical difficulties of existing technologies, such as the inability of *Anoectochilus roxburghii* to grow robustly and low survival rates during the high-temperature season after June, thereby increasing the fresh / dry weight and the content of medicinal active substances in *Anoectochilus roxburghii*.

[0028] The method of this invention can reduce labor and facility costs, and facilitate management and harvesting. Detailed Implementation

[0029] This invention discloses a method for cultivating Anoectochilus roxburghii (a type of orchid) to withstand high temperatures, which includes the following specific steps:

[0030] (1) Seedling selection: Seedlings should be selected from tissue culture seedlings that have been cultured in the culture room for 3-4 months, with a plant height of 5.0-6.0cm, and have well-developed and robust root systems. When tissue culture seedlings are transferred from the tissue culture room to a ventilated, covered seedling box, the large change in environment can easily cause death during transplanting. Therefore, before planting, the tissue culture seedlings of *Anoectochilus roxburghii* need to be moved to a greenhouse, where the temperature is controlled below 30℃ and the light intensity is about 4000-5000 lx, to harden them off. This will enhance their adaptability to the greenhouse environment after being removed from the culture bottle and improve the survival rate. The hardening-off period is generally about 25 days. After hardening-off, the seedlings are ready for transplanting when the leaves are fully expanded, the stems are light red in color and have a high degree of fibrosis.

[0031] (2) Planting equipment: The breathable seedling box includes: 1. Breathable seedling cover: transparent plastic, 150mm high × 550mm long × 300mm wide, with two sets of ventilation holes, located on the left and right halves respectively. Each group has four 6mm diameter holes, each located in one-quarter of a circular area (i.e., a 90-degree sector). Each group has a closed cover, meaning all four holes share a large circular closed cover. The cover can be closed or opened simultaneously. After closing, there is a ventilation gap (e.g., a 1-3mm gap, achieved by methods such as adding protrusions at intervals along the lower edge of the closed cover for support, or using a snap-on flip cover with the snap points protruding slightly from the surface) to allow for water and air permeability. 2. Seedling tray: A rectangular plastic seedling tray with a mesh bottom, 60mm high × 540mm long × 280mm wide, with 32 holes per tray, holding 32 seedlings. 3. Water storage tray: 65mm high × 540mm long × 285mm wide. In use, the bottom of the seedling tray is placed inside the water storage tray (because the holes in the seedling tray gradually decrease in size from top to bottom, it is easy to insert). Then, cover the top of the seedling tray with the ventilated, closed cover. During the planting process, the round, closed cover should not be opened. When spraying, water can flow into the tray through the gap between the closed cover and the tray surface to replenish a small amount of water. It also allows for slight ventilation, primarily to prevent bacterial or fungal contamination from outside the tray.

[0032] (3) Selection of cultivation substrate: The cultivation substrate is peat moss: perlite: nutrient solution = 6:3:1, mixed evenly; each 1L of nutrient solution contains Flower Treasure No. 2, amino oligosaccharide, Bacillus subtilis powder, and trace element fertilizer. The peat moss is from the Finnish brand Kejira, with particles of 2-8mm and a pH of 5.5-6.5.

[0033] (4) Planting method using a planter: Fill the seedling tray with the mixed substrate from step (3), with a substrate depth of 5.5–6 cm. Gently remove the *Anoectochilus roxburghii* tissue culture seedlings from step (1) from the bottle, and wash the culture medium from the roots, avoiding root damage or breakage during washing. Soak the tissue culture seedlings in a 500-fold diluted 50% carbendazim solution for 3 minutes for disinfection, then air dry them in a cool place. Plant them in rectangular plastic seedling trays and compact them slightly. Place a water storage tray filled with purified water into the seedling trays, adding 3 g of slow-release fertilizer (effective for 180 days) to each water storage tray. Cover with a breathable, sealed lid and close the closed lid.

[0034] (5) Planting and maintenance: Place the planter with the golden thread lotus seedlings planted in step (4) above the greenhouse seedbed, with a spacing of 10×10cm between planters. No additional watering or fertilization is required during the planting and maintenance process. Only a shading rate of 70-80% is needed (light intensity controlled at 3000-5000 lx), and automatic spraying for 10 minutes at noon.

[0035] The nutrient components added to the cultivation substrate are as follows: 8-12 ml of amino oligosaccharide, 18-22 g of Flower Treasure No. 2, 8-12 g of Bacillus subtilis powder, 1.5-2.5 g of nitrogen (N), 0.8-1.2 g of phosphorus pentoxide (P2O5), 1.5-2.5 g of potassium oxide (K2O), 0.02-0.04 g of magnesium oxide (MgO), 0.015-0.025 g of boron (B), 0.002-0.004 g of zinc (Zn), 0.045-0.065 g / L of arginine, 0.005-0.030 g / L of Mo, 0.050-0.090 g / L of Mn, 0.3-0.5 g / L of Fe, 0.8-1.2 g / L of SO3, and 0.01-0.03 g / L of Cu. g / L; the nutrients are distributed in groups, and the reagents are dissolved separately in sterile water, mixed evenly, and then mixed with the cultivation substrate.

[0036] In a specific embodiment of the invention, the tissue-cultured seedlings are complete plants with a height of 5.0-6.0 cm, a root length of 2.0-4.0 cm, and 2-3 roots. Acclimatization and hardening conditions are as follows: greenhouse temperature 22-28℃, shading rate 70-80% (light intensity controlled at 3000-5000 lx), and hardening is carried out by closing the seedlings for 10-15 days, partially opening them for 2-3 days, and fully opening them for 3-5 days. Each planting device contains 32 seedlings. The planting devices containing the *Anoectochilus roxburghii* seedlings are placed above the greenhouse seedbed, with a spacing of 10×10 cm between planting devices.

[0037] To further illustrate the method of the present invention, the applicant provides the following examples of several varieties of Anoectochilus roxburghii, and these examples are merely exemplary and are not intended to limit the scope of protection of the present invention.

[0038] Example 1

[0039] (1) Several tissue culture seedlings: Seven varieties of Anoectochilus roxburghii tissue culture seedlings of healthy growth and free from pests and diseases were selected from the Tissue Culture Laboratory of Traditional Chinese Medicine of Fujian Subtropical Botany Institute as seedlings. These seedlings were: 1. Guizhou, 2. Taiwan, 3. Guangxi, 4. Longyan (Hongxia), 5. Fujian (Nanjing) large-leaf green round leaf, 6. Fujian (Nanjing) large-leaf red round leaf, and 7. Ningde Zhouning small-leaf variety. They were planted in the medicinal plant greenhouse of Fujian Subtropical Botany Institute in April 2024. The greenhouse was not cooled.

[0040] (2) Planting equipment: The breathable and enclosed seedling box includes: 1. Breathable and enclosed cover: transparent plastic, 150mm high × 550mm long × 300mm wide, with two sets of ventilation holes, each set with 4 holes of 6mm diameter, and each set with a closed cover; 2. Seedling tray: rectangular plastic seedling tray with mesh bottom, 60mm high × 540mm long × 280mm wide, each seedling tray with 32 holes, planting 32 seedlings; 3. Water storage tray, 65mm high × 540mm long × 285mm wide.

[0041] (3) Cultivation substrate: The cultivation substrate is peat moss: perlite: nutrient solution = 6:3:1, mixed evenly; each 1L of nutrient solution contains 20g of Flower Treasure No. 2, 10g of Bacillus subtilis powder, and trace element fertilizer. The peat moss is from the Finnish brand Kejira, with particles of 2-8mm and a pH of 5.5-6.5. More specifically, the nutrient solution components added to the cultivation substrate are shown in Table 1: the nutrient solutions are dissolved separately in sterile water according to the component ratio, mixed evenly, and then mixed with the cultivation substrate.

[0042] (4) Planting method using a planter: Fill the seedling tray with the mixed substrate from step (3), with a substrate depth of 5.5-6cm. Gently remove the tissue culture seedlings of *Anoectochilus roxburghii* from the bottle in step (1), and wash the culture medium off the roots, avoiding root damage or breakage during washing. Soak the tissue culture seedlings in a 1000-fold diluted 30% oxadixyl solution for 1 minute for disinfection, then air dry them in a cool place. Plant them in rectangular plastic seedling trays and compact them slightly. Place a water storage tray filled with purified water into the seedling tray (pre-fill the water storage tray with 3-5cm of water, and the water storage tray will be full after the seedling tray is placed in). Add 3g of slow-release fertilizer (effective for 180 days) to each water storage tray. Cover with a breathable, sealed lid and close the closed lid.

[0043] (5) Planting and maintenance: Place the planter with the golden thread lotus seedlings planted in step (4) above the greenhouse seedbed, with a spacing of 10×10cm between planters. No additional watering or fertilization is required during the planting and maintenance process. Only a shading rate of 70-80% is needed (light intensity controlled at 3000-5000 lx), and automatic spraying for 10 minutes at noon.

[0044] Table 1. Composition of the nutrient solution and dosage of each component in Example 1

[0045]

[0046] The planting period was from April 1, 2024 to September 30, 2024. The extreme high temperature outside the greenhouse was 38℃, and the extreme high temperature inside the greenhouse was 43℃. The average monthly high temperature outside the greenhouse was 31℃ for about 4 months. The planting results are shown in Tables 2 to 5.

[0047] Table 2. Growth indicators of different varieties of Anoectochilus roxburghii after summering

[0048]

[0049] Table 3. Average fresh and dry weight and dry weight percentage of different varieties of Anoectochilus roxburghii after summering in Example 1.

[0050]

[0051] Table 4. Average active ingredient indexes of different varieties of Anoectochilus roxburghii after summering in Example 1

[0052]

[0053] Table 5. Survival rate of different varieties of Anoectochilus roxburghii after summer in Example 1

[0054]

[0055] Example 2

[0056] (1) Several tissue culture seedlings: Seven varieties of Anoectochilus roxburghii tissue culture seedlings of healthy growth and free from diseases and pests were selected from the Tissue Culture Laboratory of Traditional Chinese Medicine of Fujian Subtropical Botany Institute as seedlings. These seedlings were: 1. Guizhou, 2. Taiwan, 3. Guangxi, 4. Longyan (Hongxia), 5. Fujian (Nanjing) large-leaf green round leaf, 6. Fujian (Nanjing) large-leaf red round leaf, and 7. Ningde Zhouning small-leaf variety. They were planted in the medicinal plant greenhouse of Fujian Subtropical Botany Institute in April 2025. The greenhouse was not cooled.

[0057] (2) Planting equipment: The breathable and enclosed seedling box includes: 1. Breathable and enclosed cover: transparent plastic, 150mm high × 550mm long × 300mm wide, with two sets of ventilation holes, each set with 4 holes of 6mm diameter, and each set with a closed cover; 2. Seedling tray: rectangular plastic seedling tray with mesh bottom, 60mm high × 540mm long × 280mm wide, each seedling tray with 32 holes, planting 32 seedlings; 3. Water storage tray, 65mm high × 540mm long × 285mm wide.

[0058] (3) Cultivation substrate: The cultivation substrate is peat moss: perlite: nutrient solution = 6:3:1, mixed evenly; each 1L of nutrient solution contains 20g of Flower Treasure No. 2, 10g of Bacillus subtilis powder, and trace element fertilizer. The peat moss is from the Finnish brand Kejira, with particles of 2-8mm and a pH of 5.5-6.5. More specifically, the nutrient solution components added to the cultivation substrate are shown in Table 2; the nutrients are dissolved separately in sterile water according to the group ratio, mixed evenly, and then mixed with the cultivation substrate.

[0059] (4) Planting method using a planter: Fill the seedling tray with the mixed substrate from step (3), with a substrate depth of 5.5–6 cm. Gently remove the *Anoectochilus roxburghii* tissue culture seedlings from step (1) from the bottle, and wash the culture medium from the roots, avoiding root damage or breakage during washing. Soak the tissue culture seedlings in a 1000-fold diluted 30% oxadixyl solution for 1 minute for disinfection, then air dry them in a cool place. Plant them in rectangular plastic seedling trays and compact them slightly. Place a water storage tray filled with purified water into the seedling tray, adding 3 g of slow-release fertilizer (effective for 180 days) to each water storage tray. Cover with a breathable, sealed lid and close the lid.

[0060] (5) Planting and maintenance: Place the planter with the golden thread lotus seedlings planted in step (4) above the greenhouse seedbed, with a spacing of 10×10cm between planters. No additional watering or fertilization is required during the planting and maintenance process. Only a shading rate of 70-80% is needed (light intensity controlled at 3000-5000 lx), and automatic spraying for 10 minutes at noon.

[0061] Table 6. Composition of the nutrient solution and dosage of each component in Example 2

[0062]

[0063] The planting period was from April 3, 2025 to September 30, 2025. The extreme outdoor temperature was 37℃, and the extreme indoor temperature was 42℃. The average monthly outdoor temperature of 30℃ lasted for approximately 4 months. The results are shown in Tables 7 to 10.

[0064] Table 7 Growth indicators of different varieties of Anoectochilus roxburghii after summer in Example 2

[0065]

[0066] Table 8. Average fresh and dry weight and dry weight percentage of different varieties of Anoectochilus roxburghii after summering in Example 2.

[0067]

[0068] Table 9. Average active ingredient indexes of different varieties of Anoectochilus roxburghii after summering in Example 2

[0069]

[0070] Table 10 Survival rate of different varieties of Anoectochilus roxburghii after summer in Example 2

[0071]

[0072] The results of Examples 1-2 show that: (1) the method of the present invention can safely survive the summer in low-altitude and high-temperature areas, and the survival rate of the plant after one year is over 95%; the height, number of leaves, and length of each node of Anoectochilus roxburghii are the same as those of other suitable temperature seasons; the average fresh weight, average dry weight, and dry weight of Anoectochilus roxburghii are the same as those of other suitable temperature seasons; the active ingredients of Anoectochilus roxburghii: total flavonoids, total alkaloids, and Anoectochilus roxburghii glycosides are slightly lower than those of other suitable temperature seasons; the total amino acids, total polysaccharides, and total phenols are slightly higher than those of other suitable temperature seasons.

[0073] This invention describes a high-temperature resistant cultivation method for *Anoectochilus roxburghii* (a type of orchid), enabling it to safely survive four months of high temperatures and maintain healthy growth. Through a comprehensive approach including a semi-enclosed planting device, sterilization, substrate preparation, nutrient solution addition, tissue culture seedling transplantation, and greenhouse facilities, the method achieves the goal of ensuring *Anoectochilus roxburghii* survives four months of continuous high temperatures and maintains healthy growth. Using a semi-enclosed planting device effectively controls diseases. Without it, stem rot typically develops after two months of sustained high temperatures, leading to the death of the *Anoectochilus roxburghii*. Once disease occurs, it cannot be controlled by medication. Cultivating the seedlings in a ventilated, enclosed seedling box with a closed lid effectively prevents disease invasion and provides the necessary light and CO2 conditions for *Anoectochilus roxburghii* growth. This invention also includes a 10-minute automatic spray system at midday daily, which cleans the ventilated, enclosed seedling box, effectively preventing disease invasion, provides cooling, and replenishes a small amount of moisture within the seedling box through the closed lid.

[0074] This invention utilizes greenhouse shading with a shading rate of 70-80% (light intensity controlled at 3000-5000 lx), ensuring relatively uniform light exposure, which is more conducive to the growth of *Anoectochilus roxburghii*. By adding a specific nutrient solution, *Anoectochilus roxburghii* can withstand high temperatures without additional fertilization, simplifying management and meeting its nutritional needs for a longer period.

[0075] This invention employs a "semi-enclosed planter" (a sealed cultivation cover with openable and closable ventilation holes, a seedling tray, and a water storage tray) to form a microenvironmental control system. The initial sealed state, combined with periodic ventilation, creates a stable space with high humidity, low light, and minimal temperature fluctuations. This perfectly simulates the cool and humid environment under the original forest cover of *Anoectochilus roxburghii*, fundamentally isolating the plants from direct invasion by hot, dry air, effectively preventing wilting due to excessive transpiration from high temperatures. Simultaneously, this physical isolation constitutes the first line of defense, greatly reducing the chances of invasion by external pathogens and pests. Brief spraying at midday cools the exterior of the sealed cultivation cover while replenishing internal humidity through the gaps in the closed cover, achieving a dynamic balance in the microenvironment.

[0076] This invention not only boasts a high survival rate but also significantly reduces management costs and operational complexity. Its "foolproof" management greatly saves labor costs: throughout the high-temperature season, the growth of *Anoectochilus roxburghii* relies primarily on a semi-enclosed microenvironment and water-storage trays for water and fertilizer supply, eliminating the need for additional watering and soil fertilization. This completely frees management personnel from tedious daily watering and fertilization work, requiring only shade net management and simple automatic sprinkler systems. This makes large-scale, industrialized cultivation possible in low-altitude, high-temperature areas, greatly lowering the technical threshold and labor costs. Compared to traditional greenhouse cultivation that relies on expensive cooling equipment such as air conditioners, fans, and wet curtains, this invention only requires standard greenhouses and shade nets, achieving physical cooling through a semi-enclosed system, avoiding high equipment purchase costs and electricity bills.

[0077] In summary, the high-temperature-resistant cultivation method for *Anoectochilus roxburghii* provided by this invention successfully overcomes the industry-level challenge of *Anoectochilus roxburghii*'s inability to safely survive the summer in low-altitude, high-temperature regions through a systematic solution integrating specific equipment, specialized substrates, refined processes, and environmental control. As shown in the example data, *Anoectochilus roxburghii* cultivated using this method exhibits excellent growth indicators such as plant height, number of leaves, fresh weight, and dry weight, with guaranteed active ingredients: key medicinal components such as total polysaccharides and total amino acids are maintained at high levels, ensuring the effectiveness of *Anoectochilus roxburghii* as a valuable medicinal material.

Claims

1. A method for growing high-temperature-resistant Anoectochilus roxburghii, characterized by, The method can make the Anoectochilus roxburghii grow safely under the condition of the average monthly high temperature of 30-35 DEG C for 3-5 months in summer, and the survival rate is not less than 95%, by constructing a semi-closed microenvironment and combining specific cultivation management measures; the method comprises the following steps in sequence: Step one: preparation and sterilization of the planting device: a semi-closed planting device is provided, which is composed of an openable and closable breathable cover, a seedling tray with a water-permeable net at the bottom, and a water storage tray supporting the seedling tray; the breathable cover has a height of 140-160 mm, a length of 530-570 mm, and a width of 290-310 mm, and is provided with at least two groups of air-permeable holes, each group comprising 3-5 air-permeable holes with a diameter of 5-8 mm and being provided with an openable and closable cover; the seedling tray has a depth of 55-65 mm; before use, the components of the planting device are cleaned and sterilized; Step two: preparation of the cultivation substrate: a cultivation substrate is prepared by mixing and stirring the base cultivation medium peat soil, perlite and nutrient solution in a volume ratio of (5-7):(2-4):(0.8-1.2); the peat soil has a pH value of 5.0-7.0 and a particle size of 1-10 mm; Step three: sterilization and tray filling of the substrate: the prepared cultivation substrate in step two is filled into the seedling tray to a depth of 5.0-6.5 cm, and the surface of the substrate is sprayed with a carbendazim solution with a concentration of 4.0-12.0 mg / L for sterilization; Step four: pretreatment and planting of the tissue culture seedlings: healthy Anoectochilus roxburghii tissue culture seedlings with a height of 4.5-7.0 cm, a root length of 1.5-5.0 cm, and 2-4 roots are selected, taken out from the culture container after acclimatization, washed to remove the culture medium attached to the roots, and soaked in a 50% carbendazim aqueous solution diluted by 400-600 times for 2-4 minutes or in a 30% dimetachlone aqueous solution diluted by 800-1200 times for 1-2 minutes for sterilization, and then planted in the seedling tray filled with the sterilized substrate in step three after drying; Step five: closing of the planting device and water management: the seedling tray planted with the Anoectochilus roxburghii seedlings is placed in the water storage tray, water with a depth of 3-5 cm is injected into the water storage tray, 2-4 g of slow-release fertilizer with a valid period of 150-210 days is added, then the breathable cover is covered and the air-permeable holes are closed to form an initial closed and moist environment; Step six: greenhouse facilities and environmental control: the semi-closed planting device prepared in step five is placed in a greenhouse, and the distance between the planting devices is set to 8-12 cm; the greenhouse is provided with a shading net with a shading rate of 65%-85%, the light intensity is controlled to be 2500-5500 Lux, and an automatic spraying system is set to spray for 8-12 minutes during the high-temperature period from 11 o'clock to 14 o'clock every day for cooling; during the whole high-temperature season, the growth of the Anoectochilus roxburghii mainly relies on the semi-closed microenvironment and the water and fertilizer supplied by the water storage tray, and no additional watering and soil fertilization operations are required.

2. The high-temperature-resistant planting method of Anubias barteri according to claim 1, characterized in that, The seedling tray in step one is a rectangular hole tray, with a length of 530-550 mm and a width of 270-290 mm, and 28-36 planting holes are arranged on the tray.

3. The high-temperature-resistant planting method of Chrysopidium aureum according to claim 1, characterized in that, The water storage tray in step one has a height of 60-70 mm, a length of 530-550 mm, and a width of 280-290 mm.

4. The high-temperature-resistant planting method of Chrysopidium aureum according to claim 1, characterized in that, In step one, a gap of 1-3 mm for water and air permeation is arranged between the bottom of the closed cover and the upper surface of the breathable cover.

5. The high-temperature-resistant planting method of Chrysopidium aureum according to claim 4, characterized in that, During planting, the closed cover is not opened.

6. The high-temperature-resistant planting method of Chrysopidium aureum according to claim 1, characterized in that, The nutrient solution in step two contains the following components per liter: amino oligosaccharide 8-12 ml, Huabao No. 2 18-22 g, Bacillus subtilis powder 8-12 g, and trace element fertilizer 4-10 g.

7. The high-temperature-resistant planting method of Chrysopidium aureum according to claim 6, characterized in that, In the nutrient solution in step two, 0.045-0.065 g of arginine is also included per liter; and the trace element fertilizer in the nutrient solution includes nitrogen 1.5-2.5 g, phosphorus pentoxide 0.8-1.2 g, potassium oxide 1.5-2.5 g, magnesium oxide 0.02-0.04 g, boron 0.015-0.025 g, zinc 0.002-0.004 g, Mo 0.005-0.030 g, Mn 0.050-0.090 g, Fe 0.3-0.5 g, SO3 0.8-1.2 g, and Cu 0.01-0.03 g.

8. The method for cultivating *Anoectochilus roxburghii* to withstand high temperatures according to claim 1, characterized in that, In step four, the seedling conditions are: environmental temperature 20-30℃, shading rate 65-85%, and light intensity controlled at 2500-5500 lux. The seedling process includes closed culture for 8-18 days, semi-open culture for 2-4 days, and full open culture for 2-6 days.

9. The application of the high-temperature-resistant planting method of Anoectochilus roxburghii according to any one of claims 1 to 8 in the industrial planting of Anoectochilus roxburghii in low-altitude and high-temperature areas.

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