Composite microecological preparation for improving intestinal immunity of broiler chickens

Abstract

The invention relates to the technical field of broiler chicken feeding, in particular to a compound microecological preparation for improving the intestinal immunity of broiler chickens, which comprises the following components in parts by weight: 10-30% of bacillus subtilis; 10% to 30% of lactobacillus acidophilus; 10% to 30% of bifidobacterium; 10%-30% of bacillus licheniformis; 10%-20% of saccharomyces cerevisiae; 5%-20% of lactobacillus reuteri; the method solves the problems that in the traditional broiler feeding process, antibiotics are used for improving the broiler intestinal immunity, consequently, broiler intestinal flora imbalance is caused, and public health is endangered.

Description

Technical Field

[0001] This invention relates to the field of broiler breeding technology, and more specifically to a compound microecological preparation for improving the intestinal immunity of broilers. Background Technology

[0002] In modern intensive broiler farming, due to factors such as high density, rapid growth and environmental stress, broilers are prone to intestinal flora imbalance, which can lead to intestinal diseases such as diarrhea, coccidiosis, and Escherichia coli infection, seriously affecting growth performance and survival rate.

[0003] To prevent and treat the above problems, antibiotic growth promoters (such as chlortetracycline and enrofloxacin) are commonly added to traditional feed. This method has a certain antibacterial effect, but it also has the following drawbacks: antibiotics are not completely metabolized in animals and are prone to remain in tissues such as muscles and liver. After entering the human body, they may cause allergies, dysbiosis, or the spread of drug resistance, endangering public health; although antibiotics can inhibit bacteria, they do not act selectively and often destroy beneficial intestinal flora at the same time, leading to flora imbalance and weakening the intestinal barrier and local immune system. Summary of the Invention

[0004] The purpose of this invention is to provide a compound microecological preparation to improve the intestinal immunity of broilers, which solves the problem of intestinal flora imbalance and endangering public health caused by the use of antibiotics in the traditional broiler breeding process to improve intestinal immunity.

[0005] The technical solution adopted by this invention to solve its technical problem is: This invention provides a compound microecological preparation for improving intestinal immunity in broilers, comprising the following formula in parts by weight: Bacillus subtilis: 10%-30%; Lactobacillus acidophilus: 10%-30%; Bifidobacteria: 10%-30%; Bacillus licheniformis: 10%-30%; Brewing yeast: 10%-20%; Lactobacillus reuteri: 5%-20%.

[0006] Furthermore, the viable counts of the Bacillus subtilis, Lactobacillus acidophilus, Bifidobacterium, Bacillus licheniformis, Saccharomyces cerevisiae, and Lactobacillus reuteri strains are all 5×10⁹ to 1×10¹⁰ CFU / g.

[0007] Furthermore, the Bacillus subtilis was cultured at 35°C in MRS medium for 36 hours under aerobic conditions.

[0008] Furthermore, the Lactobacillus acidophilus was cultured at 37°C in MRS medium for 48 hours under anaerobic conditions.

[0009] Furthermore, the Bifidobacterium was cultured at 37°C in TPY medium for 48 hours under anaerobic conditions.

[0010] Furthermore, the Bacillus licheniformis was cultured at 35°C in MRS medium for 40 hours under aerobic conditions.

[0011] Furthermore, the brewing temperature of the brewing yeast is 28°C, the culture medium is YPD medium, and it is cultured in an aerobic environment for 24 hours.

[0012] Furthermore, the Lactobacillus reuteri was cultured at 37°C in MRS medium for 48 hours under anaerobic conditions.

[0013] Technical effects of the present invention: Compared with existing technologies, the compound microecological preparation for improving the intestinal immunity of broilers involved in this invention can significantly improve the intestinal microecological environment of broilers, enhance immunity, inhibit harmful bacteria, and promote nutrient absorption, thereby improving the production performance of broilers. It has the potential to replace antibiotics, avoids the problem of incomplete metabolism of antibiotics in animals, and avoids harming public health. In addition, this preparation can maintain beneficial intestinal flora and maintain the intestinal flora imbalance of broilers. Detailed Implementation

[0014] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0015] Example 1: The compound microecological preparation for improving intestinal immunity in broilers described in this embodiment includes the following formula in parts by weight: Bacillus subtilis: 10%, the culture temperature of Bacillus subtilis is 35℃, the culture medium is MRS medium, and it is cultured in an aerobic environment for 36h; Lactobacillus acidophilus: 15%, the culture temperature of Lactobacillus acidophilus is 37℃, the culture medium is MRS medium, and it is cultured in an anaerobic environment for 48h; Bifidobacterium: 20%, the culture temperature of the Bifidobacterium was 37℃, the culture medium was TPY medium, and it was cultured in an anaerobic environment for 48h; Bacillus licheniformis: 20%, the culture temperature of Bacillus licheniformis is 35℃, the culture medium is MRS medium, and it is cultured in an aerobic environment for 40h; Saccharomyces cerevisiae: 20%, the culture temperature of the Saccharomyces cerevisiae is 28℃, the culture medium is YPD medium, and it is cultured in an aerobic environment for 24h; Lactobacillus reuteri: 15%, the culture temperature of Lactobacillus reuteri was 37℃, the culture medium was MRS medium, and it was cultured in an anaerobic environment for 48h.

[0016] The viable counts of the Bacillus subtilis, Lactobacillus acidophilus, Bifidobacterium, Bacillus licheniformis, Saccharomyces cerevisiae, and Lactobacillus reuteri strains were all 5 × 10⁻⁶. 9 ~1×10 10 CFU / g; After cultivation, the above-mentioned bacterial strains are centrifuged, the bacterial sludge is collected, and it is suspended and dried at low temperature. The bacterial powder is then mixed in a certain proportion and used evenly at a ratio of 500g of the bacterial powder per ton of feed.

[0017] Example 2: The compound microecological preparation for improving intestinal immunity in broilers described in this embodiment includes the following formula in parts by weight: Bacillus subtilis: 20%, the culture temperature of Bacillus subtilis is 35℃, the culture medium is MRS medium, and it is cultured in an aerobic environment for 36h; Lactobacillus acidophilus: 20%, the culture temperature of Lactobacillus acidophilus is 37℃, the culture medium is MRS medium, and it is cultured in an anaerobic environment for 48h; Bifidobacterium: 20%, the culture temperature of the Bifidobacterium was 37℃, the culture medium was TPY medium, and it was cultured in an anaerobic environment for 48h; Bacillus licheniformis: 10%, the culture temperature of Bacillus licheniformis is 35℃, the culture medium is MRS medium, and it is cultured in an aerobic environment for 40h; Saccharomyces cerevisiae: 20%, the culture temperature of the Saccharomyces cerevisiae is 28℃, the culture medium is YPD medium, and it is cultured in an aerobic environment for 24h; Lactobacillus reuteri: 10%, the culture temperature of Lactobacillus reuteri was 37℃, the culture medium was MRS medium, and it was cultured in an anaerobic environment for 48h.

[0018] The viable counts of the Bacillus subtilis, Lactobacillus acidophilus, Bifidobacterium, Bacillus licheniformis, Saccharomyces cerevisiae, and Lactobacillus reuteri strains were all 5 × 10⁻⁶. 9 ~1×10 10 CFU / g; After cultivation, the above-mentioned bacterial strains are centrifuged, the bacterial sludge is collected, and it is suspended and dried at low temperature. The bacterial powder is then mixed in a certain proportion and used evenly at a ratio of 500g of the bacterial powder per ton of feed.

[0019] Example 3: The compound microecological preparation for improving intestinal immunity in broilers described in this embodiment includes the following formula in parts by weight: Bacillus subtilis: 30%, the culture temperature of Bacillus subtilis is 35℃, the culture medium is MRS medium, and the culture is carried out in an aerobic environment for 36 hours; Lactobacillus acidophilus: 20%, the culture temperature of Lactobacillus acidophilus is 37℃, the culture medium is MRS medium, and it is cultured in an anaerobic environment for 48h; Bifidobacterium: 10%, the culture temperature of the Bifidobacterium was 37℃, the culture medium was TPY medium, and it was cultured in an anaerobic environment for 48h; Bacillus licheniformis: 10%, the culture temperature of Bacillus licheniformis is 35℃, the culture medium is MRS medium, and it is cultured in an aerobic environment for 40h; Saccharomyces cerevisiae: 15%, the culture temperature of the Saccharomyces cerevisiae is 28℃, the culture medium is YPD medium, and it is cultured in an aerobic environment for 24h; Lactobacillus reuteri: 15%, the culture temperature of Lactobacillus reuteri was 37℃, the culture medium was MRS medium, and it was cultured in an anaerobic environment for 48h.

[0020] The viable counts of the Bacillus subtilis, Lactobacillus acidophilus, Bifidobacterium, Bacillus licheniformis, Saccharomyces cerevisiae, and Lactobacillus reuteri strains were all 5 × 10⁻⁶. 9 ~1×10 10 CFU / g; After cultivation, the above-mentioned bacterial strains are centrifuged, the bacterial sludge is collected, and it is suspended and dried at low temperature. The bacterial powder is then mixed in a certain proportion and used evenly at a ratio of 500g of the bacterial powder per ton of feed.

[0021] Comparative Example 1: Add 200g of chlortetracycline per ton of feed and mix evenly.

[0022] Experimental procedure: Six hundred 1-day-old Avian white-feathered broiler chickens in good health and with similar weight were selected and randomly divided into five groups of 60 birds each (A, B, C, D, and E) without any drug prophylaxis. Each group had four replicates of 15 chickens each. Group A was the control group 1, which was fed a basal diet of corn and soybean meal. Group B was the control group 2, which was fed the diet obtained in Comparative Example 1. Groups C, D, and E were the experimental groups, which were fed the diets obtained in Examples 1, 2, and 3, respectively. The rearing period was 42 days. The chickens were raised in a fully enclosed floor-house environment with clean drinking water and free access to feed.

[0023] The detection indicators and methods are shown in Table 1: Indicator Classification detection indicators Method Description Growth performance Daily gain (ADG) and feed conversion ratio (FCR) Weigh and record feed intake weekly. Immune indicators IgA, IgG, IL-6, TNF-α Serum was collected and tested using ELISA. Intestinal structure Fiber height, crypt depth, VH / CD ratio Dissecting the ileum segment, measuring under a microscope after HE staining. Microbial community analysis Escherichia coli and lactic acid bacteria count Intestinal contents dilution plating culture counting method Table 1 Experimental results: 1. Comparison of growth performance: Group Daily weight gain (g) FCR (Feed Conversion Rate) Group A 48.7 1.83 Group B 52.4 1.69 Group C 55.6 1.60 Group D 57.4 1.55 Group E 56.7 1.56 Table 2 2. Serum immune markers (day 42): index Group A Group B Group C Group D Group E IgA (mg / mL) 1.23 1.56 1.62 1.73 1.68 IgG (mg / mL) 7.41 8.93 9.35 9.49 9.38 IL-6 (pg / mL) 15.6 10.3 8.2 7.6 7.9 TNF-α (pg / mL) 17.4 12.2 10.3 9.6 9.4 Table 3 Intestinal morphology analysis: Group Pile height (μm) crypt depth (μm) VH / CD ratio Group A 826 162 5.10 Group B 954 138 6.91 Group C 986 126 7.83 Group D 1013 111 9.13 Group E 975 130 7.50 Table 4 4. Gut microbiota count (cfu / g): fungus Group A Group B Group C Group D Group E E. coli <![CDATA[3.2 × 10 6 ]]> <![CDATA[1.8 × 10 6 ]]> <![CDATA[1.3 × 10 6 ]]> <![CDATA[1.0× 10 6 ]]> <![CDATA[1.2× 10 6 ]]> Lactic acid bacteria <![CDATA[4.5 × 10 7 ]]> <![CDATA[8.9 × 10 7 ]]> <![CDATA[9.3× 10 7 ]]> <![CDATA[9.8× 10 7 ]]> <![CDATA[9.2 × 10 7 ]]> Table 5 Conclusion: As shown in Tables 2-5, this compound microecological preparation can significantly improve the intestinal microecological environment of broilers, enhance immunity, inhibit harmful bacteria, and promote nutrient absorption, thereby improving the production performance of broilers. It has the potential to replace antibiotics, avoiding the problem of incomplete metabolism of antibiotics in animals and harming public health. In addition, this preparation can maintain beneficial intestinal flora and maintain the intestinal flora imbalance of broilers.

[0024] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or basic characteristics. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

[0025] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A compound microecological preparation for enhancing intestinal immunity in broilers, characterized in that, Formulas comprising the following parts by weight: Bacillus subtilis: 10%-30%; Lactobacillus acidophilus: 10%-30%; Bifidobacteria: 10%-30%; Bacillus licheniformis: 10%-30%; Brewing yeast: 10%-20%; Lactobacillus reuteri: 5%-20%.

2. The compound microecological preparation for improving intestinal immunity in broilers according to claim 1, characterized in that: The viable counts of the Bacillus subtilis, Lactobacillus acidophilus, Bifidobacterium, Bacillus licheniformis, Saccharomyces cerevisiae, and Lactobacillus reuteri strains were all 5 × 10⁻⁶. 9 ~1×10 10 CFU / g.

3. The compound microecological preparation for improving intestinal immunity in broilers according to claim 1, characterized in that: The Bacillus subtilis was cultured at 35°C in MRS medium for 36 hours under aerobic conditions.

4. The compound microecological preparation for improving intestinal immunity in broilers according to claim 1, characterized in that: The Lactobacillus acidophilus was cultured at 37°C in MRS medium for 48 hours under anaerobic conditions.

5. The compound microecological preparation for improving intestinal immunity in broilers according to claim 1, characterized in that: The Bifidobacterium was cultured at 37°C in TPY medium for 48 hours under anaerobic conditions.

6. The compound microecological preparation for improving intestinal immunity in broilers according to claim 1, characterized in that: The Bacillus licheniformis was cultured at 35°C in MRS medium for 40 hours under aerobic conditions.

7. The compound microecological preparation for improving intestinal immunity in broilers according to claim 1, characterized in that: The brewing yeast was cultured at 28°C in YPD medium for 24 hours under aerobic conditions.

8. The compound microecological preparation for improving intestinal immunity in broilers according to claim 1, characterized in that: The Lactobacillus reuteri was cultured at 37°C in MRS medium for 48 hours under anaerobic conditions.