Herbicidal composition for machine-transplanted rice seedling field and application thereof

Abstract

The invention belongs to the field of pesticide chemistry application, and particularly relates to application of a weeding composition for machine-transplanted rice seedling fields to prevention and removal of resistant moleplant seeds, the weeding composition is composed of active components pyraclonil, clomazone and butachlor, the weight ratio of pyraclonil to clomazone to butachlor is 1: 1-3: 20-40, and the preferable weight ratio is 1: 1: 20. Emulsion in water and granules are prepared through dosage form processing, the emulsion in water is uniformly diluted with water and then applied, and the granules are mixed with fertilizer or directly applied mechanically or manually. The pesticide is applied before rice soil preparation and rice transplanting or after rice transplanting and living, and is used for solving the problem of resistance moleplant weeds which are difficult to prevent and control in a machine-transplanted rice seedling field.

Description

Technical Field

[0001] This invention belongs to the field of agricultural chemical application technology, specifically relating to a weed control composition for rice machine-transplanted fields for controlling resistant barnyardgrass. It is composed of active ingredients such as cypermethrin, isoxaflutole, and butachlor, and is applied to control resistant barnyardgrass and annual weeds in machine-transplanted fields before land preparation or after transplanting. Background Technology

[0004] Pyraclonil is a rice herbicide with a novel pyrazolidine ring structure, developed by Bayer AG of Germany and Kyoyyu Agriculture of Japan. It is a protoporphyrinogen oxidase (PPO) inhibitor that inhibits the activity of PPO, preventing the utilization of protoporphyrin IX in the cytoplasm and thus affecting plant photosynthesis. Pyraclonil is absorbed through the roots and leaf bases of weeds, causing the accumulation of PPO in the plant's nerves to exert its herbicidal effect. Weeds treated with the herbicide exhibit symptoms such as leaf curling, yellowing flowers, and wilting, eventually drying out and dying. Pyraclonil shows good activity against resistant / rugged weeds in rice paddies, including *Impatiens balsamina*, *Eriocaulon buergerianum*, *Gnaphalium affine*, *Gnaphalium affine*, and *Sagittaria sagittifolia*, but its effectiveness against barnyardgrass is less than ideal.

[0005] Isoxaflutole is an isoxazoline herbicide developed by FMC. Isoxaflutole is a carotenoid biosynthesis inhibitor, belonging to the selective pre-emergence herbicide class. It inhibits the synthesis of pentadiene compounds, thus hindering the biosynthesis of carotene and chlorophyll. It is absorbed through the plant's roots and young shoots, transported upwards, and diffuses through the xylem to the leaves. Although susceptible weeds may germinate and emerge, they lack pigment and become white seedlings, eventually dying within a short period.

[0006] Butachlor is a selective systemic amide herbicide that is mainly absorbed through the young shoots and small secondary roots of weeds. It inhibits cell growth by blocking protein synthesis and can control annual weeds such as barnyard grass, Echinochloa crus-galli, Cyperus difformis, and Echinochloa chinensis. However, due to the frequent use of amides and cross-resistance issues over the past 20 years, its control effect on barnyard grass, Echinochloa crus-galli, and Cyperus difformis has become less than ideal.

[0007] A search on the China Patent Network yielded no results related to the use of a ternary compound herbicidal composition of bispyribac-sodium, isoxaflutole, and butachlor for weed control in machine-transplanted rice fields. Summary of the Invention

[0008] The purpose of this invention is to provide a weed control composition for machine-transplanted rice fields, which can solve the problem of controlling resistant Echinochloa crus-galli in machine-transplanted rice fields.

[0009] To fully illustrate the advantages of the present invention, the following technical solution is provided.

[0010] A weed control composition for rice transplanting fields is used to control resistant Echinochloa crus-galli, characterized in that: the active ingredients are a mixture of bispyribac-sodium, isoxaflutole, and butachlor, wherein the weight ratio of bispyribac-sodium, isoxaflutole, and butachlor is 1:1-3:20-40.

[0011] The application described in this invention is characterized in that the preferred weight ratio of the composition of bispyribac-sodium, isoxaflutole, and butachlor is 1:1:20.

[0012] The application described in this invention is characterized in that the composition of bispyribac-sodium, isoxaflutole, and butachlor is processed into granules or emulsions according to pesticide formulation processes.

[0013] The application described in this invention is characterized in that the total weight percentage of the composition of the compound containing bispyribac-sodium, isoxaflutole, and butachlor is 0.5-80%.

[0014] The application described in this invention is characterized in that the total weight percentage of the composition of the compound containing bispyribac-sodium, isoxaflutole, and butachlor is 1-40%.

[0015] The application described in this invention is characterized in that the composition is applied before machine transplanting or after the rice seedlings have established themselves.

[0016] The application described in this invention is characterized in that the composition is used to solve the problem of annual weeds in machine-transplanted rice seedlings.

[0017] The application described in this invention is characterized in that the composition is used to solve the problem of difficulty in controlling resistant Echinochloa crus-galli weeds in machine-transplanted rice fields.

[0018] Compared with the prior art, the present invention has the following superior effects.

[0019] The herbicidal composition of the present invention, consisting of bispyribac-sodium, isoxaflutole, and butachlor, exhibits a synergistic effect when used in combination to antagonize Echinochloa crus-galli.

[0020] The herbicidal composition of the present invention, a mixture of bispyribac-sodium, isoxaflutole, and butachlor, is significantly more effective than the control agents butachlor, the mixture of bispyribac-sodium and isoxaflutole, the mixture of bispyribac-sodium and butachlor, and the mixture of butachlor and isoxaflutole in controlling the herbicide *Echinochloa crus-galli*.

[0021] The herbicide composition of this invention can be applied to rice paddies before or after transplanting, which can solve the current problem of resistant Echinochloa crus-galli weed infestation and eliminate the problems of large dosage and high cost of foliar herbicides.

[0022] The herbicidal composition of this invention, when applied to machine-transplanted rice fields, can broaden the spectrum of weed control and solve the problem of annual weed infestation. Detailed Implementation

[0023] The superior effects of the present invention will be described in detail with reference to the following embodiments, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0024] Example 1: Synergistic effect assay of resistant *Echinochloa crus-galli*: The reagents were provided or prepared by Zhejiang Tianfeng Biotechnology Research Institute. The reagents included 16% bispyribac-sodium suspension (without synergistic adjuvants), 60% butachlor microemulsion (without safener), and 40% isoxaflutole emulsifiable concentrate (without synergistic adjuvants).

[0025] Target: Resistant Echinochloa crus-galli was collected from a rice-transplanting field in Gaoyou, Jiangsu Province in 2022. Before transplanting, 150g / mu of 60% butachlor microemulsion was applied to the field, and after transplanting, 400g / mu of 6% pyrimisulfuron-methyl·pretilachlor·quinclorac acid GR + 400g / mu of 0.3% penoxsulam granules were applied. At the time of rice harvest, large areas of Echinochloa crus-galli remained in the field.

[0026] Experimental method: Select impermeable pots and weigh out an equal amount of untreated silt, spreading it evenly inside. Then, evenly sprinkle resistant *Echinochloa crus-galli* into the pots. After two days, when the *Echinochloa crus-galli* is in the germination stage, apply the herbicide according to the following plan. The day after application, select pre-cultivated 4-leaf stage rice seedlings for transplanting. Observe the weed control effect and the safety to rice.

[0027] To make the experiment more closely resemble field data, the entire process, from transplanting and applying pesticides to observation, was carried out outdoors.

[0028] Observe the weed control efficacy 15 days after application. The evaluation method is based on the Colby formula. A, B, and C represent the actual weed survival rate (%) of a single agent; E represents the theoretical survival rate (%); E-E0≤-10% indicates synergistic effect; -10%>E-E0<10% indicates additive effect; E-E0≥10% indicates antagonistic effect. Table 1. Combined toxicity determination of bispyribac-sodium, isoxaflutole, and butachlor against antagonistic *Echinochloa crus-galli* 15 days after application. The interaction between the pot and bowl experiments shows that: Bismuth subtilis 1 ga.i / 667m 2 The antagonistic efficacy against Echinochloa crus-galli is 20%, and the isoxaflutole 1-2-3 ga.i / 667m 2 The occlusive efficacy is 0-5-15%, butachlor 20-30-40 g.i / 667m 2 The effectiveness of the closed system is 0-15-24%.

[0029] The combination of bispyribac-sodium, isoxaflutole, and butachlor has a significant synergistic effect on the pre-emergence of resistant Echinochloa crus-galli. The combination of the three can effectively reduce the amount of pesticides used and slow down the development of herbicide resistance in weeds.

[0030] Example 2: Safety test of bispyribac-sodium, isoxaflutole, and butachlor on rice The test reagents, targets, and test methods are the same as in Example 1. Table 2. Evaluation of the safety and herbicidal efficacy of bispyribac-sodium, isoxaflutole, and butachlor on rice 20 days after application. The experiment shows that: 1) The combination of bispyribac, isoxaflutole, and butachlor under the given dosage conditions achieved 100% control efficacy against resistant Echinochloa crus-galli, which was significantly higher than the control efficacy of bispyribac + isoxaflutole, bispyribac + butachlor, and isoxaflutole + butachlor in combination.

[0031] 2) The weight ratio of bispyribac-isoxamethonium:isoxamethonium:butachlor is 1:1:20-40, which is safe for rice. Increasing the dosage of isoxamethonium can easily cause whitening of rice, and may further cause dwarfing in mixed varieties.

[0032] Based on Examples 1 and 2 and in accordance with the Ministry of Agriculture and Rural Affairs' policy on reducing pesticide use and residues, the preferred weight ratio of the mixture of bispyribac-sodium, isoxaflutole, and butachlor is 1:1:20.

[0033] Example 3: Formulation preparation of 66% bispyribac-isoxazone-butachlor emulsion (1:1:20) Bisoxam 3%, Isoxaflutole 3%, Butachlor 60%, Dispersogen LFS (phosphate dispersant, Clariant, Switzerland) 1%, Tensiofix 96DB10 (EO / PO block polyether emulsifier, Shanghai Tesk Chemical Co., Ltd.) 1%, Propylene glycol 3%, SAG1522 (silicone defoamer, Momentive, USA) 0.02%, Water balance.

[0034] Processing method: Mix bispyribac, isoxaflutole, and butachlor in a certain proportion until bispyribac is completely dissolved to obtain the oil phase; mix Dispersogen LFS, Tensiofix 96DB10, propylene glycol, Sag1522, and water in a certain proportion to obtain the aqueous phase; add the oil phase to the aqueous phase at a rate of 50 kg / min while shearing, and continue shearing for 30 minutes after the addition is complete to obtain 66% bispyribac·isoxaflutole·butachlor water emulsion.

[0035] Example 4: 44% Bisoxam·Isoxam·Butachlor emulsion (1:1:20). Bisoxam 2%, Isoxaflutole 2%, Butachlor 40%, Dispersogen LFS 1%, Tensiofix 96DB101%, Propylene Glycol 3%, SAG1522 0.02%, Water balance.

[0036] Processing method: Same as in Example 4.

[0037] Example 5: 11% Bisoxam·Isoxam·Butachlor Granules (1:1:20) Bisoxam 0.5%, isoxaflutole 0.5%, butachlor 10%, silica 3%, agricultural emulsion 500# (calcium dodecylbenzene sulfonate, Nantong Deyi Chemical Co., Ltd.) 2%, Tensiofix EW70 (EO / PO block polyether emulsifier, Shanghai Tesk Chemical Co., Ltd.) 2%, and silica sand balance.

[0038] Processing method: Mix bispyribac, isoxaflutole, butachlor, agricultural emulsion 500#, and Tensiofix EW70 in proportion until bispyribac is completely dissolved to obtain an oil phase; add quartz sand to a stirred tank, then add the oil phase while stirring. After adding the oil phase, add silica and kaolin, and stir until no visible oil phase remains.

[0039] Note: The dosage form processing techniques in Examples 3-5 of this invention are well known to those skilled in the art. The differences between the formulations mainly come from the percentage of active ingredients in the total mass and the ratio between active ingredients. The types and amounts of excipients are kept as consistent as possible, with adjustments only made to the ratio of lipophilic to hydrophilic properties to reduce unnecessary variables in the experiments.

[0040] Example 6 Field Plot Experiment The experiment was conducted in Gaoyou, Jiangsu Province, using the Huajing No. 5 rice variety, with seedlings 21 days old and at 3.5 leaves. The field was divided into small plots, with ridges erected to prevent waterlogging between plots. After leveling each plot, 150g / 667m² of 60% butachlor microemulsion was manually and evenly applied while the water was still turbid. 2 On the third day after pesticide application, rice seedlings were transplanted using a riding-type rice transplanter. After the seedlings had established themselves (on the 7th day after transplanting), a manual application test was conducted according to the following plan.

[0041] Note: Before applying the pesticide, manually remove any remaining weeds after the first application.

[0042] Table 3. Field survey results on rice safety and weed control effectiveness. The experiment shows that: 1) Treatment plan: Apply 11% cypermethrin·isoxane·butachlor granules (1:1:20) after land preparation and transplanting, at a dosage of 200-300-400g / 667m². 2 It is safe for rice, and its control efficacy against barnyard grass, duckweed, and wild vegetable is 100%, 100%, 100%, and 100%, respectively.

[0043] 2) The dosage of 11% bispyribac-isoxazone-butachlor granules (1:1:20) is 200-300-400g / 667m³. 2 The control efficacy against barnyard grass, duckweed, and wild clover was significantly better than that of the control agents 2% bispyribac-isoxamine granules, 21% bispyribac-butachlor granules, and 21% isoxachlor-butachlor granules.

[0044] 3) The dosage of 11% bispyribac-isoxazone-butachlor granules (1:1:20) is 200-300-400g / 667m³. 2 It has a significantly wider weed control spectrum than the control agent, which helps to solve the problem of weed infestation during machine transplanting in one go.

[0045] Example 7 Field Plot Experiment The experiment was conducted in Jurong City, Jiangsu Province, using the rice variety Suxiangjing 100, with seedlings 19 days old and at the 3.5-leaf stage. The field was divided into small plots, with ridges erected to prevent waterlogging between plots. After leveling each plot, the pesticide was applied manually and evenly while the water was still turbid. On the third day after application, rice seedlings were transplanted using a riding-type rice transplanter. The safety of the rice and the weed-control effect were observed after the pesticide application.

[0046] Table 4. Field survey results on rice safety and weed control effectiveness. The experiment showed that: 1) the treatment scheme of 44% bispyribac-isoxane-butachlor emulsifiable concentrate (1:1:20) was applied before land preparation and transplanting, with a dosage of 80g / 667m². 2 For rice, the control efficacy against barnyard grass, styrax, and sedge was 82%, 98%, 86%, and 95%, respectively; the application rate was 100-150g / 667m³. 2 For rice, the control efficacy against barnyard grass, styrax, and sedge was 100%, 100%, 100%, and 100%, respectively.

[0047] 2) Apply 44% bispyribac-isoxane-butachlor emulsifiable concentrate (1:1:20) before transplanting, at a dosage of 80-150g / 667m². 2 It is safe for rice and its weed control effect on barnyard grass, styrax, and sedge is significantly better than that of 60% butachlor microemulsion.

[0048] 3) Apply 44% bispyribac-isoxane-butachlor emulsifiable concentrate (1:1:20) before transplanting, at a dosage of 80-150g / 667m². 2 It is safe for rice and has a significantly wider weed control spectrum than butachlor-based pre-emergence weed control schemes. The ternary compound formulation helps to solve the weed problem in machine-transplanted rice in one go.

[0049] The above embodiments are all intended to demonstrate preferred embodiments of the present invention. Any modifications, equivalent substitutions, or alterations made within the spirit and principles of the present invention should be included within the scope of protection of the present invention. It is also worth noting that the dosage form processing techniques of the present invention are all prior art and are well known to those skilled in the art.

Claims

1. An application of a weed control composition for controlling resistant Echinochloa crus-galli in rice transplanting fields, characterized in that: The active ingredients consist of bispyribac-sodium, isoxaflutole, and butachlor, wherein the weight ratio of bispyribac-sodium, isoxaflutole, and butachlor is 1:1-3:20-40.

2. The application according to claim 1, characterized in that: The preferred weight ratio of bispyribac-sodium, isoxaflutole, and butachlor is 1:1:

20.

3. The application according to claim 2, characterized in that: The herbicidal composition of bispyribac-sodium, isoxaflutole, and butachlor is processed into granules and emulsions according to pesticide formulation technology.

4. The application according to claim 3, characterized in that: The weight ratio of bispyribac-sodium, isoxaflutole, and butachlor to the total weight of the composition is 0.5-80%.

5. The application according to claim 4, characterized in that: The weight ratio of bispyribac-sodium, isoxaflutole, and butachlor to the total weight of the composition is 1-40%.

6. The application according to claims 1-5, characterized in that: The herbicidal composition is applied before or after the rice seedlings have established themselves for machine transplanting.

7. The application according to claims 1-6, characterized in that: The herbicidal composition described herein is used to control annual weeds in machine-transplanted rice fields.

8. The application according to claims 1-7, characterized in that: The herbicidal composition described herein is used to address the problem of resistant Echinochloa crus-galli weed infestation that is difficult to control in machine-transplanted rice fields.

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