Intestinal nutrition regulator containing laminarin and preparation method thereof

Abstract

The invention relates to the technical field of biological medicines, in particular to an intestinal nutrition regulator containing laminarin and a preparation method of the intestinal nutrition regulator. The intestinal nutrition regulator containing the laminarin is prepared from the laminarin, probiotics and auxiliary materials through scientific proportioning and a unique process. The preparation method comprises the following steps: firstly, efficiently extracting polysaccharide from kelp through a process of combining ultrasonic-assisted enzyme extraction (UEE) and acid-assisted extraction (AAC); meanwhile, by exerting the synergistic effect of the laminarin, the silymarin extract and the resveratrol, the composition can effectively improve the intestinal flora structure, enhance the integrity of the intestinal barrier, inhibit the growth of pathogenic bacteria and regulate immune response. The intestinal nutrition regulator can be used for preparing food or health care products for regulating and / or improving intestinal flora balance of mammals, has good effects on preventing and improving intestinal related diseases such as intestinal dysfunction, irritable bowel syndrome and inflammatory bowel disease, and has wide market prospect and application value.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological medicine, and particularly relates to an intestinal nutrition regulator containing laminarin and a preparation method thereof. BACKGROUND

[0002] With the acceleration of modern life pace and the change of dietary structure, intestinal health problems are increasingly prominent, such as intestinal flora imbalance, constipation, diarrhea, low immunity, etc. As an important tool for maintaining and improving intestinal health, the market demand for intestinal nutrition regulators continues to grow. Existing intestinal nutrition regulators mainly include prebiotics, probiotics, dietary fiber supplements, etc. The commonly used prebiotics such as fructooligosaccharides (FOS), galactooligosaccharides (GOS) and inulin, although they can promote the growth of beneficial bacteria, their sources are relatively single, the functional targeting is limited, and some products may have poor tolerance, excessive use leading to abdominal distension and other problems. In addition, many traditional dietary fiber regulators mainly focus on the physical function of defecation, and are insufficient in the aspects of regulating immunity, anti-inflammatory, repairing intestinal mucosa and other multifunctional synergies.

[0003] Laminaria japonica, also known as kelp, is a perennial edible alga with medicinal and edible properties, rich in nutrients such as amino acids, vitamins, trace elements and polysaccharides, and has the effects of reducing swelling, eliminating phlegm and treating iodine deficiency. Laminarin widely exists in kelp cells and is one of the main active ingredients, with various biological activities such as free radical scavenging, blood glucose lowering, bacteriostasis, anti-inflammatory and anticancer, and has good application prospects in the development of functional foods and auxiliary drugs. Among them, laminarin has been confirmed by modern research to have various biological activities, for example, short-chain fatty acids (SCFAs) produced by polysaccharide metabolism can inhibit the generation of harmful substances in the intestine and inhibit the growth of harmful bacteria, playing an important role in maintaining host health and preventing diseases (Lin Xiaojuan, Su Zhichen, Chen Jicheng. Structural characteristics, biological activity and application of laminarin [J]. Modern Food, 2021, (24): 49-52. DOI:10.16736 / j.cnki.cn41-1434 / ts.2021.24.014.). At the same time, the intervention of laminarin can change the intestinal microbiota by increasing the polysaccharide bacteria in the digestive diet and reducing potential pathogenic bacteria, effectively improving the intestinal microbiota imbalance caused by high-fat diet, suggesting that laminarin can be used as a food supplement to improve energy homeostasis balance (Lin Huiting, Wang Peixin, Lai Bin, et al. Functional activity and application research progress of laminarin [J]. Food Research and Development, 2020, 41 (14): 188-195.). Therefore, laminarin has become an ideal candidate for the development of new multifunctional intestinal nutrition regulators.

[0004] However, there are still many challenges in directly applying seaweed polysaccharides to intestinal nutrients, for example, in the preparation process of seaweed polysaccharides, the traditional water extraction method has low extraction rate and low purity, and the active structure of polysaccharides is easily damaged at high temperature. At the same time, the broad-spectrum and effectiveness of intestinal regulation of single seaweed polysaccharides may not be as good as complex formulations. Therefore, developing an efficient and safe extraction method that can maximize the intestinal health benefits of seaweed polysaccharides through scientific compounding has positive significance for filling the gaps in existing technologies and providing a new type of efficient intestinal nutrient regulator. SUMMARY

[0005] To solve the above technical problems, the purpose of the present application is to provide a kind of intestinal nutrition regulator containing seaweed polysaccharide and its preparation method, to solve the problems of extraction of effective components of existing seaweed polysaccharides and single function of traditional intestinal nutrition regulator.

[0006] To achieve the above technical effects, the present application adopts the following technical solutions: In a first aspect, an intestinal nutrition regulator containing seaweed polysaccharide includes the following active ingredients: 20-50 parts of seaweed polysaccharide extract, 5-10 parts of silymarin extract, and 2-5 parts of resveratrol.

[0007] Further, the intestinal nutrition regulator further includes: 5-100 parts of freeze-dried probiotic powder, and the probiotics in the freeze-dried probiotic powder are Bifidobacterium and / or Lactobacillus.

[0008] Further, the probiotics are one or more of animal Bifidobacterium, Bifidobacterium lactis and Lactobacillus rhamnosus.

[0009] Preferably, the probiotics are a combination of animal Bifidobacterium and Bifidobacterium lactis, and the ratio of viable bacteria of the two is 1:0.5-1:2. Preferably, the intestinal nutrition regulator further includes an excipient, and the excipient includes one or more of a filler and an anti-caking agent; the filler is one or more of resistant dextrin, microcrystalline cellulose and malt dextrin; and the anti-caking agent is silicon dioxide.

[0010] Preferably, the dosage form of the intestinal nutrition regulator is any one of powder, granules, tablets or capsules.

[0011] Further, the extraction of seaweed polysaccharide extract used in the intestinal nutrition regulator containing seaweed polysaccharide includes the following steps: (1) ultrasonic-assisted enzyme extraction: dry and crush fresh kelp, pass through an 80-120 mesh sieve to obtain dried kelp powder, mix the kelp powder with an acetic acid-sodium acetate buffer solution at pH 4.0-5.0 at a solid-liquid ratio of 1:15-1:25 (w / v), add a compound enzyme, and perform enzymatic extraction at an ultrasonic power of 600-1000 W, a frequency of 40 kHz, and a temperature of 45-55℃ for 1-3 hours, then inactivate the enzyme in a water bath at 90-95℃ for 10-15 minutes to obtain an extraction system I; (2) acid-assisted extraction: add an acid solution to the extraction system I, adjust the pH of the system to 3.0-5.0, preferably 3.0-4.0, and stir at 50-70℃ for 2-4 hours to obtain an extraction system II; (3) post-treatment: neutralize the extraction system II to a pH of 6.8-7.2, concentrate under reduced pressure to 1 / 4-1 / 3 of the original volume to obtain a concentrated solution, then add 70% ethanol (volume fraction) at 3-4 times the volume of the concentrated solution, stand at 0-4℃ for 8-12 hours, collect the precipitate, wash the precipitate 2-3 times, then dry and crush to obtain the kelp polysaccharide extract.

[0012] Further, the compound enzyme in (1) is a mixture of cellulase and pectinase at a mass ratio of 1:2-1:4, and the addition amount is 1%-3% of the mass of the kelp powder.

[0013] Further preferably, the cellulase has an enzyme activity of not less than 3000 U / g, and the pectinase has an enzyme activity of not less than 10000 U / g.

[0014] Further, the acid solution in step (2) is a 0.05-0.2 mol / L hydrochloric acid solution; the extraction temperature of the heating extraction is 50-70℃, and the extraction time of the heating extraction is 2-4 hours.

[0015] In a second aspect, the present application also provides a preparation method of the intestinal nutritional regulator containing kelp polysaccharide as described above, which specifically comprises the following steps: a. prepare or obtain kelp polysaccharide extract, silymarin extract, resveratrol, and freeze-dried probiotic powder, respectively; b. pre-mixing: mix the freeze-dried probiotic powder with at least part of the fillers in the excipients to obtain a probiotic pre-mixture; c. main mixing: uniformly mix the kelp polysaccharide extract, silymarin extract, and resveratrol with the remaining excipients except the anti-caking agent to obtain a main mixture; d. final mixing: gently mix the probiotic pre-mixture with the main mixture for 10-15 minutes, finally add the anti-caking agent and mix uniformly, then perform dispensing or packaging to obtain the product.

[0016] In a third aspect, the present application further provides a use of the intestinal nutrition regulator containing laminarin provided by the first aspect in the preparation of a food or health product for regulating and / or improving the intestinal flora balance of a mammal.

[0017] Compared with the prior art, the present application has the following beneficial effects: Firstly, the present application scientifically matches laminarin, silymarin extract, resveratrol and probiotics to form a synergistic effect. Laminarin, as a prebiotic, forms a "synbiotic" effect with probiotics. Meanwhile, the anti-inflammatory and antioxidant properties of silymarin extract and resveratrol and the intestinal regulation function of probiotics promote each other, significantly enhancing the intestinal nutrition regulation effect.

[0018] In addition, the present application uses a method combining ultrasonic-assisted enzyme extraction and acid-assisted extraction to extract laminarin. Ultrasonic treatment improves the cell wall crushing efficiency, and compound enzymolysis can specifically degrade the cell wall structure of laminaria, significantly improving the extraction rate and purity of laminarin, while retaining more active ingredients, and the extraction efficiency is obviously improved compared with traditional methods. In summary, the present application uses natural plant extracts and probiotics as the main active ingredients, has no chemical additives, is safe and non-toxic without side effects for long-term use, and is suitable for people of all ages for long-term use. The present application can be used to prepare a food or health product for regulating and / or improving the intestinal flora balance of a mammal, and has good effects on preventing and improving intestinal function disorders, irritable bowel syndrome, inflammatory bowel disease and other intestinal related diseases, and has a broad market prospect and application value. DETAILED DESCRIPTION

[0019] The following examples are only used to more clearly illustrate the technical solutions of the present application, and therefore only serve as examples, but cannot limit the protection scope of the present application.

[0020] Those skilled in the art should understand that the present application can also be implemented without certain specific details. In some other embodiments, methods, means, apparatus and steps familiar to those skilled in the art are not described in detail in order to highlight the main idea of the present application. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as generally understood by those skilled in the art. Unless otherwise specified, the units used in the specification are international standard units, and the numerical values and numerical ranges appearing in the present application should be understood to include the systematic errors inevitable in industrial production.

[0021] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials, reagents or instruments used, if not specified by the manufacturer, are reagents and materials available from commercial channels; if the specific conditions are not specified in the examples, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer; meanwhile, the source of the raw materials used in the present application is not limited, and if not specifically stated, the raw materials used in the present application are ordinary commercially available products in the technical field. If not specifically stated, the "ratio" referred to in the following examples is the ratio of mass parts.

[0022] Example 1 The purpose of this example is to compare the effects of traditional hot water extraction, ultrasonic-assisted enzyme extraction, conventional enzymatic hydrolysis + acid extraction, and ultrasonic-assisted enzyme extraction-directed acid-controlled degradation (UEE-AAC) process with different acid hydrolysis times on the yield of kelp polysaccharide, molecular weight characteristics, and in vitro prebiotic activity. The experiments are as follows: 1.1 Partial experimental materials Fresh kelp: collected from the sea area of Rongcheng, Shandong, washed, and removed of mud and impurities, then dried at 60°C with a blast dryer until constant weight, crushed and passed through an 80-mesh sieve to make kelp powder, which was sealed and stored away from light; Reagents: cellulase (purchased from Shanghai Yuan Ye Bio) and pectinase (purchased from Beijing Solaybao Technology); Fecal samples: fresh feces of healthy adults (no history of intestinal diseases, and no antibiotics taken in the past 3 months) were processed within 1h of collection.

[0023] 1.2 Preparation of kelp polysaccharide The group settings of this example are shown in Table 1:

[0024] Table 1 Methods for extracting kelp polysaccharide in each group

[0025] Kelp polysaccharides were prepared by extracting kelp polysaccharides from the same batch of raw materials according to the different methods in Table 1.

[0026] 1.3 Activity detection methods and results of kelp polysaccharide The detection indicators include: (1) The yield of kelp polysaccharide was calculated according to the following method: the mass of kelp polysaccharide after freeze-drying / the initial mass of kelp powder × 100%; (2) Molecular weight characteristics: determined by GPC-MALLS, with 0.1 mol / L NaNO3 solution as the mobile phase, a flow rate of 0.5 mL / min, a column temperature of 35°C, and the weight average molecular weight (Mw) and main distribution range (calculated based on the molecular weight range of 85% of the components) were recorded; (3) In vitro prebiotic activity: 5 mg of Laminaria polysaccharide samples prepared by different preparation methods were mixed with 10 mL of healthy human fecal flora suspension (feces: sterile normal saline = 1:10, w / v), and placed in a 37°C anaerobic incubator for 24h culture; After culture, the pH, total SCFAs and butyric acid content (GC method), and the growth of bifidobacterium (qPCR method, with the blank control group as the benchmark) of the fermentation broth were determined.

[0027] The yield and molecular weight of Laminaria polysaccharides obtained by different extraction methods were repeated three times, and the results are shown in Table 2, and the in vitro prebiotic activity of Laminaria polysaccharides obtained by different extraction methods is shown in Table 3:

[0028] Table 2 Yield and molecular weight of Laminaria polysaccharides obtained by different extraction methods

[0029] The above experimental results show that: in terms of the yield of Laminaria polysaccharides, Group 5 has the highest yield (16.5%) but is not significantly different from Group 6-1 and Group 4-2, which is 101.2% higher than traditional hot water extraction (Group 1). In this group, the cell wall of Laminaria is destroyed by ultrasonic cavitation effect, and the cell wall components are degraded by complex enzymes (cellulase + pectinase), and then further dissolved by acid extraction; Compared with Group 2 (UEE) and Group 3 (conventional enzymatic hydrolysis + acid extraction), the yield is increased by 14.4% by ultrasonic treatment, which proves the promoting effect of ultrasonic on the dissolution of polysaccharides. In terms of molecular weight regulation, the acid hydrolysis time is negatively correlated with the molecular weight, that is, the longer the acid hydrolysis time, the more the polysaccharide chain is broken, and the Mw is reduced, and in terms of intestinal flora regulation, the optimal polysaccharide molecular weight for regulation needs to be further verified.

[0030] Table 3 In vitro prebiotic activity of Laminaria polysaccharides obtained by different extraction methods

[0031] The above experimental results show that: The pH of the fermentation broth was the lowest in Group 5 (5.72), because the SCFAs were produced in the highest amount, which reduced the pH of the intestinal environment, and the lower pH value could inhibit the growth of harmful bacteria such as Escherichia coli; and the pH of Group 1 (macromolecular polysaccharide) and Group 6-2 (polysaccharide with too small molecular weight) was relatively high, which proved that the prebiotic activity of the two groups was weak. At the same time, the total SCFAs (82.5 μmol / mL) of Group 5 was 116.0% higher than that of Group 1, and the butyric acid (18.3 μmol / mL) was 251.9% higher; butyric acid is the main energy source of intestinal epithelial cells and can repair intestinal mucosa, and the high yield proves the excellent prebiotic function of Group 5 polysaccharide. In terms of promoting the proliferation of Bifidobacterium, the Bifidobacterium in Group 5 increased by 8.2 times, which was significantly higher than that in other groups, and therefore, the experimental results proved that the degree of acidolysis and the molecular weight of kelp polysaccharide had a great influence on the activity, and the kelp polysaccharide with a molecular weight of 10-50 kDa could be used as a carbon source for Bifidobacterium to promote its colonization and proliferation.

[0032] Example 2 The purpose of this example is to observe the synergistic effect of kelp polysaccharide (optimal process extraction, i.e. Group 5), silymarin extract, and resveratrol after being compounded with specific probiotics, and the experiment is as follows: 2.1 Experimental materials and equipment Active ingredients: kelp polysaccharide extract (prepared by Group 5 process, purity ≥ 90%), silymarin extract (purity ≥ 90%, Shaanxi Huikexian Biological), resveratrol (purity ≥ 98%, Shanghai Maikelin Biochemical); Probiotics: animal Bifidobacterium BB-12® (Kohjin) freeze-dried powder, milk Bifidobacterium Bi-07® (Dupont) freeze-dried powder, the viable bacterial count of the two freeze-dried powders was 2×10 11 CFU / g, and the composition of probiotics in this example was that the two probiotic freeze-dried powders were mixed according to a mass ratio of 1:1 to obtain a probiotic mixture; Reagents: TNF-α ELISA detection kit (Shanghai Zhiyan Biotech), sterile normal saline, anaerobic culture medium (TSB culture medium, adding 0.5% L-cysteine); Fecal samples were the same as in Example 1.

[0033] 2.2 Experimental group setting and method Different active ingredient combinations were prepared according to the mass ratio in Table 4:

[0034] Table 4 Composition of active ingredients in different groups

[0035] The active ingredient compositions of different compositions were obtained by accurately weighing each active ingredient according to the mass ratio shown in Table 4 and mixing thoroughly. In order to strictly follow the principle of controlling variables and ensure that the observed differences in the final effects are only due to the types and ratios of active ingredients, the total mass of the "active ingredient composition" in all groups (Group 1 to Group 5) was kept consistent during each experiment. Subsequently, each equal mass of the active ingredient composition was mixed with the probiotic mixture (BB-12® and Bi-07® mixed at a mass ratio of 1:1) at a fixed mass ratio of 10:1, and after mixing evenly, it was used for in vitro fermentation experiments, as follows: Fecal suspension preparation: Take 10 g of fresh fecal sample from a healthy person, add 90 mL of sterile normal saline, vortex for 30 minutes to mix thoroughly, filter through four layers of sterile gauze, and prepare a 10% (w / v) fecal suspension.

[0036] Sample solution preparation and inoculation: The above prepared active ingredient-probiotic compound sample was prepared into a suspension with a concentration of 100 mg / mL using sterile normal saline. Then, 1 mL of each sample suspension (the blank control group was added with the same volume of sterile normal saline) was taken, mixed with 9 mL of fecal suspension and 10 mL of anaerobic culture medium (TSB medium containing 0.5% L-cysteine), as the fermentation system.

[0037] Anaerobic culture: The mixed fermentation system was placed in an anaerobic incubator (conditions: 37°C, gas environment N2:CO2:H2=85:10:5) for 24 hours.

[0038] Index determination: After the culture was completed, the samples were collected and determined by the following methods: gas chromatography-mass spectrometry (GC-MS) method was used to analyze the total short-chain fatty acids (SCFAs) and butyric acid content; high-throughput sequencing technology was used to analyze the relative abundance of Bifidobacterium and Lactobacillus; enzyme-linked immunosorbent assay (ELISA) was used to detect the content of inflammatory factor TNF-α. All experiments were repeated three times independently.

[0039] 2.3 Experimental results and analysis The results of fermentation metabolites and flora analysis experiments are shown in Table 5:

[0040] Table 5 Effect of different active ingredient compositions on fermentation metabolites and flora

[0041] The above experimental results show that: Group 1 (only kelp polysaccharide) was significantly better than the blank control group, which verified its high-quality prebiotics, providing a basis for SCFAs production and beneficial bacteria proliferation. Compared with group 1 and group 3, all indicators were further improved after 14 silymarin extracts replaced kelp polysaccharide in group 3, indicating that silymarin extract itself has the ability to enhance the effect of prebiotics and anti-inflammatory activity. The whole ingredient group (group 5) and group 3 without resveratrol had a significant increase in all indicators.

[0042] The above groups all contain a fixed amount of kelp polysaccharide as the basis of prebiotics, and the differences in indicators between groups can be clearly attributed to the addition of silymarin extract and resveratrol, as well as their specific ratio relationship among the three. Specifically: The whole ingredient combination (group 5, ratio 26:10:4) showed the best effect in all evaluation indicators, fully proving the clear synergistic effect between kelp polysaccharide, silymarin extract and resveratrol. In terms of promoting metabolic products: the total SCFAs production (96.8 μmol / mL) and butyric acid proportion (28.5%) of group 5 were the highest, significantly better than other groups. This indicates that this specific ratio not only can most effectively utilize the carbon source provided by kelp polysaccharide, but also may promote the production of butyric acid by adjusting microbial metabolic pathways. In terms of optimizing the structure of the bacterial community, the abundance of bifidobacterium (35.2%) and lactobacillus (24.6%) in group 5 also reached the peak. This shows that this combination creates the most suitable environment for the proliferation of these two core beneficial bacteria, and its effect is not simply additive, but synergistically promoted. In terms of anti-inflammatory activity, the TNF-α level (45.2 pg / mL) of group 5 was the lowest, highlighting its strongest anti-inflammatory potential. This is due to the antioxidant protection of silymarin extract and the direct anti-inflammatory activity of resveratrol, which is synergistically amplified on the basis of the improvement of the bacterial community environment by kelp polysaccharide.

[0043] In the ratio of silymarin extract and resveratrol, from group 2 to group 5, it can be clearly seen that the change of component ratio will significantly affect the final effect. Among them, group 2 (26:7:7): although it contains all three components, its effect is generally worse than group 3, group 4 and group 5, and even worse than single kelp polysaccharide group (group 1) in some indicators. This shows that the ratio of silymarin extract and resveratrol 7:7 is not optimal, and may not effectively exert a synergistic effect, or even may be inhibited or negatively affected by each other due to improper ratio. While group 3 (26:14:0) and group 4 (26:12:2): the effects of these two groups are between group 1 and group 5. Group 3 (only adding silymarin extract) performs better than group 4 in promoting SCFAs, butyric acid and beneficial bacteria, indicating that in this system, silymarin extract has a more significant synergistic effect with kelp polysaccharide when resveratrol is lacking. The effect of group 4 (containing 2 parts of resveratrol) is slightly inferior, suggesting that low-dose resveratrol has limited gains in this ratio.

[0044] The above experimental results show that there is a synergistic effect among kelp polysaccharide, silymarin extract and resveratrol after compounding. Among them, the ratio of 26 parts of kelp polysaccharide, 10 parts of silymarin extract and 4 parts of resveratrol (group 5) is the optimal combination. This combination can most effectively promote the proliferation of beneficial bacteria, increase the production of beneficial metabolites (especially butyric acid), and exert the strongest anti-inflammatory effect. There is a synergistic effect among the three, and the strength of the synergistic effect is highly dependent on the specific ratio of each component, providing a precise formula scientific basis for the development of high-efficiency intestinal microecological regulators.

[0045] Example 3 The purpose of this example is to prepare a powder type intestinal nutritional regulator that meets the standards of industrial production, has stable probiotic activity and uniform components based on the optimized kelp polysaccharide extraction process (UEE-AAC-150min) of Example 1 and the "kelp polysaccharide-silymarin extract-resveratrol-probiotic" synergistic formula verified in Example 2, as follows: 3.1 Raw material composition Active ingredients: kelp polysaccharide extract (Example 1, Group 5 process): 4.0 g, silymarin extract: 1 g, resveratrol: 0.4 g; Probiotic freeze-dried bacteria powder: 0.5 g (of which, animal bifidobacterium (BB-12®) 0.25 g, lactobifidobacterium (Bi-07®) 0.25 g) Excipients: resistant dextrin: 94 g, microcrystalline cellulose: 0.5 g and food-grade silicon dioxide: 0.5 g.

[0046] 3.2 Preparation process flow Referring to the method of Group 5 in Example 1, laminarin is prepared, and then the preparation of the intestinal nutrition regulator is carried out as follows: S1: Pretreatment: The laminarin extract, silymarin extract, and resveratrol are respectively passed through a 100-mesh sieve and prepared for use. The resistant dextrin and microcrystalline cellulose are dried in an oven at 60°C for 4 hours, and the moisture content is controlled to be ≤5%. The operating environment temperature is controlled to be ≤25°C, and the relative humidity is controlled to be ≤30%.

[0047] S2: Premixing (preparation of probiotic premix): In a sterile operating table, the probiotic freeze-dried bacteria powder and 1.5 g of resistant dextrin are placed in a mixing device, and mixed at a low speed (10 rpm) for 15 minutes to form a probiotic premix.

[0048] S3: Main mixing: The laminarin extract, silymarin extract, resveratrol, and the remaining excipients except for silicon dioxide are put into the mixing device, and mixed at a speed of 20 rpm for 20 minutes to obtain a main mixture.

[0049] S4: Final mixing: The probiotic premix is added to the main mixture, and mixed at a speed of 8 rpm for 12 minutes. Silicon dioxide is added, and the mixing is continued for 10 minutes to obtain a final mixture. The final mixture is sealed and packaged, and each bag contains 2.0 g.

[0050] The above examples are only used to illustrate the technical solutions of the present application and are not limiting. Although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present application can be modified or replaced equivalently without departing from the purpose and scope of the present application, and they should all be covered in the scope of the claims of the present application. The technical, shape, and structure parts not described in detail in the present application are well-known technologies.

Claims

1. An intestinal nutrition regulator containing kelp polysaccharides, characterized in that, Based on parts by weight, it includes the following active ingredients: 20-50 parts of kelp polysaccharide extract, 5-10 parts of silymarin extract, and 2-5 parts of resveratrol.

2. The intestinal nutrition regulator containing kelp polysaccharides as described in claim 1, characterized in that: The intestinal nutrition regulator also includes 5-100 parts of freeze-dried probiotic powder, wherein the probiotics in the freeze-dried probiotic powder are Bifidobacterium and / or Lactobacillus.

3. The intestinal nutrition regulator containing kelp polysaccharides as described in claim 2, characterized in that: The probiotics are one or more of Bifidobacterium animalis, Bifidobacterium lactis, and Lactobacillus rhamnosus.

4. The intestinal nutrition regulator containing kelp polysaccharides as described in claim 1, characterized in that: The intestinal nutrition regulator also includes excipients, which include one or more of fillers and anti-caking agents; the filler is one or more of resistant dextrin, microcrystalline cellulose, and maltodextrin; and the anti-caking agent is silicon dioxide.

5. An intestinal nutrition regulator containing kelp polysaccharides as described in any one of claims 1-4, characterized in that: The dosage form of the intestinal nutrition regulator is any one of powder, granules, tablets or capsules.

6. An intestinal nutrition regulator containing kelp polysaccharides as described in any one of claims 1-5, characterized in that, The extraction of the kelp polysaccharide extract includes the following steps: (1) Ultrasonic-assisted enzyme extraction: Fresh kelp is dried and then pulverized and passed through an 80-120 mesh sieve to obtain dried kelp powder. The kelp powder is mixed with an acetate-sodium acetate buffer solution with pH 4.0-5.0 at a material-liquid ratio of 1:15-1:25 (w / v). A compound enzyme is added, and the mixture is enzymatically extracted for 1-3 hours under ultrasonic power of 600-1000W, frequency of 40kHz, and temperature of 45-55℃. Then, the enzyme is inactivated in a water bath at 90-95℃ for 10-15 minutes to obtain extraction system I. (2) Acid-assisted extraction: Add acid solution to extraction system I, adjust the pH value of the system to 3.0-5.0, and stir and extract for 2-4 hours at 50-70℃ to obtain extraction system II; (3) Post-processing: Neutralize extraction system II to pH 6.8 ~ 7.2, concentrate under reduced pressure to 1 / 4-1 / 3 of the original volume to obtain a concentrate, then add 3-4 times the volume of 70% ethanol to the concentrate, let stand at 0-4℃ for 8-12 hours, collect the precipitate, wash the precipitate 2-3 times, and then dry and pulverize to finally obtain the kelp polysaccharide extract.

7. The intestinal nutrition regulator containing kelp polysaccharides as described in claim 6, characterized in that: The compound enzyme in (1) is a mixture of cellulase and pectinase in a mass ratio of 1:2 to 1:4, and the amount added is 1% to 3% of the mass of kelp powder.

8. The intestinal nutrition regulator containing kelp polysaccharides as described in claim 6, characterized in that: The acid solution in step (2) is a 0.05-0.2 mol / L hydrochloric acid solution; the extraction temperature of the heating extraction is 50-70℃, and the extraction time of the heating extraction is 2-4 hours.

9. The use of the kelp polysaccharide-containing intestinal nutrition regulator according to any one of claims 1-8 in the preparation of food or health products for regulating and / or improving the balance of intestinal flora in mammals.

10. A method for preparing an intestinal nutrition regulator containing kelp polysaccharides as described in any one of claims 1-5, characterized in that, Includes the following steps: a. Prepare or obtain kelp polysaccharide extract, silymarin extract, resveratrol, and freeze-dried probiotic powder, respectively; b. Premixing: The freeze-dried probiotic powder is mixed with at least a portion of the filler in the excipients to obtain a probiotic premix; c. Main Mixing: The kelp polysaccharide extract, silymarin extract, resveratrol, and other excipients except anti-caking agents are mixed evenly to obtain the main mixture; d. Final mixing: Gently mix the probiotic premix with the main mixture for 10-15 minutes, then add the anti-caking agent and mix evenly. Finally, dispense or package the product.

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