Aeromonas rapid detection kit

Abstract

The application relates to an Aeromonas rapid detection kit in the technical field of Aeromonas, which comprises a top base and a bottom base, an inner shell is fixed between the top base and the bottom base, an annular cover is rotationally connected to the outer wall of the top base through a bearing, the bottom of the annular cover is fixed with an outer shell which is attached to the outer wall of the inner shell, a multi-station assembly is arranged in the inner shell, a driving mechanism for driving the multi-station assembly to step-rotate is arranged on the top base, and a pressure applying assembly is partially arranged on the top base and partially extends into the inner shell. The application can process multiple samples at a time, the detection efficiency is greatly improved compared with a single-channel detection mode, the to-be-detected sample is rapidly diffused and penetrates the Aeromonas test paper under the action of airflow blowing and pressure, the problem of slow sample diffusion in traditional test paper detection is solved, and the requirement of rapid detection is met.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of aeromonas, in particular to a rapid detection kit for aeromonas. BACKGROUND

[0002] Aeromonas is a kind of gram-negative bacteria widely distributed in aquatic environment, and its sub-strains such as Aeromonas hydrophila and Aeromonas veronii have strong pathogenicity, which can cause fish hemorrhagic septicemia, human acute gastroenteritis and other diseases, and pose a serious threat to aquaculture, food safety and public health. At present, the existing detection methods of aeromonas mainly include traditional culture method, PCR technology and the like, but all have obvious defects: the traditional culture method takes 2-5 days for detection, which cannot meet the requirement of rapid prevention and control; the PCR technology relies on expensive instruments and professional personnel, and has poor applicability at the grassroots level. In order to facilitate rapid detection, the existing technology also generally uses a kit for direct detection, that is, the sample to be detected is added dropwise on the aeromonas test paper in the kit, and after a period of time, the sample to be detected is naturally penetrated on the test paper, then the test paper is aligned with the result comparison card, and the color development is observed. However, although the aeromonas test paper detection method realizes the convenient operation of "sample dropwise addition", it generally adopts a multi-layer laminated structure (the base layer is PVC hard sheet) of "sample pad-bonding pad-reaction membrane-water absorption pad", and the chromatography channel formed by the lamination of each layer has the problems of large penetration resistance and slow sample diffusion speed. It takes a long time for a single sample to penetrate to complete the reaction, the detection efficiency is low, and the core bottleneck of "slow speed" cannot be broken through, so the rapid detection requirement cannot be met. Therefore, a rapid detection kit for aeromonas is developed to solve the pain point of "slow speed" of the traditional detection method, and to realize the on-site rapid screening of aeromonas. SUMMARY

[0003] In view of the above prior art, the technical problem to be solved by the present application is that when the aeromonas test paper is used for detection, the penetration resistance is large and the sample diffusion speed is slow, which results in low detection efficiency and cannot meet the requirement of rapid detection.

[0004] To solve the above problems, the present application provides a rapid detection kit for aeromonas, which comprises:

[0005] A top seat and a bottom seat are fixed with an inner shell between them, and an annular cover is rotatably connected to the outer wall of the top seat through a bearing, and the bottom of the annular cover is fixed with an outer shell which is attached to the outer wall of the inner shell;

[0006] A multi-station assembly is arranged inside the inner shell, and a driving mechanism for driving the multi-station assembly to step rotate is arranged on the top seat;

[0007] A drying assembly is arranged in the middle region of the bottom seat;

[0008] A pressure applying assembly is partially arranged on the top base and partially extends into the inner shell;

[0009] An ejection assembly is arranged in the inner shell;

[0010] The top of the top base is respectively provided with a second reagent dropping opening and a second observation opening, and the top of the annular cover is respectively provided with a first reagent dropping opening and a first observation opening, the axis of the first reagent dropping opening is collinear with the axis of the second reagent dropping opening, and the axis of the first observation opening is collinear with the axis of the second observation opening;

[0011] The cross section of the second observation opening is designed as a T shape, and a colorimetric card and a magnifying glass are fixedly installed on the inner wall of the second observation opening in sequence from top to bottom;

[0012] A second taking and placing opening is arranged on one side of the inner shell, and a first taking and placing opening is correspondingly arranged on one side of the outer shell, the position of the first taking and placing opening is matched with the size of the second taking and placing opening and corresponds to the position of the second taking and placing opening.

[0013] The application further provides that the multi-station assembly comprises a rotating disc movably arranged in the inner shell, and the outer wall of the rotating disc is provided with equidistantly distributed telescopic grooves, the inner wall of each telescopic groove is slidably connected with a telescopic rod, one end of the telescopic rod is fixedly connected with a first spring at one end of the telescopic groove, and the other end of the telescopic rod is fixedly connected with a supporting cylinder, the projection of the second reagent dropping opening and the second observation opening respectively covers the central region of two adjacent supporting cylinders, a transparent carrier dish is arranged in each supporting cylinder, an air sac bacterium test paper is arranged on the transparent carrier dish, and a clamping assembly for centering and clamping the transparent carrier dish is arranged in the supporting cylinder.

[0014] The application further provides that the clamping assembly comprises an insertion groove equidistantly arranged on the inner wall of the supporting cylinder, the inner wall of the insertion groove is slidably connected with a clamping pad, the inner side surface of the clamping pad is tightly combined with the surface of the transparent carrier dish, the outer end of the clamping pad is fixedly connected with a second spring at one end of the insertion groove, and the clamping pad is made of elastic silica gel.

[0015] The application further provides that the driving mechanism comprises a mounting hole arranged at the center of the top base, the inner wall of the mounting hole is rotatably connected with a driving shaft through a sealing bearing, the rotating disc is coaxially fixedly arranged at the bottom of the driving shaft, the top of the top base is fixedly connected with a stepping motor, and the output shaft of the stepping motor is connected with the top end of the driving shaft through a shaft coupling.

[0016] The application is further provided with the installation groove on the side of the bottom of the top base, the inner wall of the installation groove is fixedly installed with the air guide cylinder, the middle of the air guide cylinder is rotatably connected with the transmission shaft, the outer wall of the transmission shaft is fixedly installed with the spiral blade which is attached to the inner wall of the air guide cylinder, the bottom of the transmission shaft is fixedly installed with the pinion, the outer wall of the driving shaft is fixedly installed with the gear, the gear is engaged with the pinion, the inner wall top of the inner shell and the bottom of the top base are fixedly installed with the annular sealing seat and the arc sealing seat which are attached to the top surface of the transparent carrier dish, the two ends of the annular sealing seat and the two ends of the arc sealing seat form two work gap, the positions of the two work gap correspond to the positions of the second reagent dropping port and the second observation port respectively, the inside of the arc sealing seat is provided with the gas collection cavity, the bottom of the gas collection cavity is provided with the exhaust hole which is distributed at equal distance, the side top of the air guide cylinder is fixedly installed with the air inlet pipe, the side bottom of the air guide cylinder and the gas collection cavity are fixedly installed with the air outlet pipe.

[0017] The application is further provided with the support rod which is fixedly installed at equal distance on the inner wall of the inner shell, the top of the support rod is fixedly installed with the same ejection disc which is composed of the disc part and the integral raised part, the position of the raised part and the position of the second taking and placing port correspond to the middle area of the second observation port and the next support cylinder respectively, the bottom of the telescopic rod is rotatably connected with the ejection wheel through the pin shaft, the ejection wheel slides along the surface of the ejection disc.

[0018] The application is further provided with the screw hole which is provided in the middle of the base, the inner wall of the screw hole is screwed with the drying box, the inside of the drying box is filled with the drying agent, the inner wall top of the drying box is fixedly installed with the leakproof mesh plate.

[0019] The application is further provided with the sealing groove which is annularly provided between the top of the top base and the inner wall of the top of the annular cover, between the inner wall of the outer shell and the top and bottom of the outer wall of the inner shell, the inner wall of the adjacent two groups of sealing grooves is attached with the sealing strip, the top of the top base is rotatably connected with the U-shaped handle frame through the pin shaft, the bottom of the base is fixedly installed with the suction disc which is distributed at equal distance.

[0020] The application is further provided with the elastic buckle which is distributed at equal distance between the outer wall of the top base and the inner wall of the annular cover, the elastic buckle is composed of the connecting groove which is provided on the outer wall of the top base and the clamping groove which is provided on the inner wall of the annular cover, the inner wall of the connecting groove is inserted with the elastic pin rod, one end of the elastic pin rod and one end of the connecting groove are fixedly installed with the third spring, the other end of the elastic pin rod is inserted in the clamping groove, the inner wall of the clamping groove and the other end of the elastic pin rod are designed as spherical surface structure, the central angle distance of the adjacent two elastic buckles, the first taking and placing port and the second taking and placing port, the first observation port and the second observation port are equal.

[0021] The top of the top seat is provided with an equipment slot on one side, and a controller and a battery are fixedly installed in the equipment slot respectively, the controller, the battery and the stepping motor are electrically connected, and a cover plate is arranged at the opening of the equipment slot.

[0022] To sum up, compared with the prior art, the application has the following advantages after adopting the above structure:

[0023] 1、In the application, the rotating disc in the multi-station assembly carries multiple transparent carrier dishes through the telescopic rods and the supporting cylinders, the aeromonas test paper is built-in, the stepping motor drives the rotating disc to step rotate through the driving shaft, and the reagent dropping port, the observation port on the top seat and the annular cover, and the taking and placing port on the outer shell and the inner shell are matched, so that the continuous performance of the processes of "dropping-reaction-observation-taking and placing" is realized, each station is accurately corresponding, manual frequent replacement of the carrier dishes and the aeromonas test paper is not needed, multiple samples can be processed at a time, compared with the single-channel detection mode, the detection efficiency is sufficiently improved.

[0024] 2、In the application, when the driving shaft rotates, the transmission shaft is driven to rotate through the meshing of the large gear and the small gear, so that the spiral blade in the air guide cylinder generates airflow, the airflow is introduced into the gas collection cavity of the arc-shaped sealing seat through the exhaust pipe, and then the airflow acts on the test paper in the transparent carrier dish through the exhaust hole, through the above design, the "station circulation" and "accelerated penetration" are performed synchronously, on the one hand, the airflow acts to blow the dropped sample to be detected, so that the sample to be detected is diffused on the aeromonas test paper, on the other hand, the airflow acts to increase the pressure in the carrier dish, so that the sample to be detected can penetrate into the aeromonas test paper more quickly, the problem of slow sample diffusion in the traditional test paper detection is solved, the sample detection time is sufficiently shortened, and the demand for rapid detection is met.

[0025] 3、In the application, the second observation port adopts a T-shaped cross-section design, and a colorimetric card and a magnifying glass are built-in, the magnifying glass can magnify the color development area of the aeromonas test paper by multiple times, so that the operator can clearly observe the color change, and the colorimetric card can be used to quickly complete result comparison, so that the problems of inconspicuous color development and large interpretation error in the traditional detection are solved.

[0026] 4、In the application, the convex part of the ejection disc corresponds to the middle area of the second observation port and the next supporting cylinder, when the rotating disc drives the supporting cylinder to move to the taking and placing port position, the ejection wheel at the bottom of the telescopic rod slides along the surface of the convex part, so that the telescopic rod drives the supporting cylinder to move to one side, when the maximum displacement is reached, the transparent carrier dish is completely ejected from the taking and placing port, so that the operator can easily take and place without stretching into the inside of the reagent box, so that the carrier dish and the aeromonas test paper can be easily cleaned and replaced, and the operation convenience is improved.

[0027] 5. In this invention, the drying box in the middle of the base is filled with desiccant, and the leak-proof mesh plate at the top can prevent the desiccant particles from falling out. At the same time, the desiccant can effectively absorb the moisture inside the reagent kit, keep the internal environment dry, avoid the test strips from becoming damp and ineffective, and extend the shelf life of the test strips.

[0028] 6. In this invention, the entire reagent kit integrates functions such as driving, pressurizing, detection, and interpretation into one unit. It does not require expensive external detection instruments. The stepper motor can be automatically controlled by the controller alone. The operation process is simplified to "sample addition - start the equipment - observe the results - pick up and put down the carrier dish". No professional operating skills are required, which solves the problem of traditional technology relying on professional personnel and equipment. It can be widely used in aquaculture sites, food processing enterprises, grassroots disease control centers and other scenarios to achieve rapid on-site screening of Aeromonas. Attached Figure Description

[0029] Figure 1 This is a three-dimensional view of the Aeromonas rapid detection kit of the present invention;

[0030] Figure 2 This is a front cross-sectional view of the Aeromonas rapid detection kit of the present invention;

[0031] Figure 3 This is a three-dimensional cross-sectional view of the Aeromonas rapid detection kit of the present invention;

[0032] Figure 4 This is a schematic diagram of the battery and second dispensing port structure of the Aeromonas hydrophila rapid detection kit of the present invention.

[0033] Figure 5 This is a schematic diagram of the sealing groove and screw hole structure of the Aeromonas rapid detection kit of the present invention;

[0034] Figure 6 This is a cross-sectional view of the outer shell of the Aeromonas rapid detection kit of the present invention;

[0035] Figure 7 This is a schematic diagram of the annular sealing seat and the arc-shaped sealing seat structure of the Aeromonas rapid detection kit of the present invention;

[0036] Figure 8 This is a top view of the rotating disk and the ejector disk of the Aeromonas rapid detection kit of the present invention;

[0037] Figure 9 This is a schematic diagram of the telescopic groove and clamping pad structure of the Aeromonas rapid detection kit of the present invention;

[0038] Figure 10 This is a bottom view of the ejector plate and arc-shaped sealing seat of the Aeromonas rapid detection kit of the present invention;

[0039] Figure 11The schematic view of the transmission shaft and helical blade structure of the aeromonas rapid detection kit of the present application is shown in the figure.

[0040] Figure 12 The exploded view of the aeromonas rapid detection kit of the present application is shown in the figure.

[0041] Explanation of the figure:

[0042] 1, top seat; 2, annular cover; 3, outer shell; 4, base; 5, first pick-and-place port; 6, first observation port; 7, first reagent dropping port; 8, controller; 9, stepper motor; 10, handle frame; 11, inner shell; 12, multi-station assembly; 121, rotating disc; 122, telescopic rod; 123, transparent carrier dish; 124, aeromonas test paper; 125, support cylinder; 126, telescopic groove; 127, first spring; 128, clamping pad; 129, second spring; 13, annular sealing seat; 14, sealing strip; 15, equipment slot; 16, drive shaft; 17, pressure applying assembly; 171, mounting groove; 172, air guide cylinder; 173, large gear; 174, air inlet pipe; 175, air outlet pipe; 176, arc-shaped sealing seat; 177, air outlet hole; 178, transmission shaft; 179, small gear; 1710, gas collection cavity; 1711, helical blade; 18, second observation port; 19, magnifying glass; 20, elastic buckle; 21, ejection assembly; 211, support rod; 212, ejection disc; 2121, disc part; 2122, protruding part; 213, ejection wheel; 22, suction disc; 23, drying box; 24, drying agent; 25, leak-proof mesh plate; 26, colorimetric card; 27, mounting hole; 28, battery; 29, second reagent dropping port; 30, second pick-and-place port; 31, sealing slot; 32, threaded hole. DETAILED DESCRIPTION

[0043] The embodiments of the present application are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as a limitation of the present application.

[0044] In the description of the present application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0045] In the description of the present application, it is necessary to point out that, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connecting", "setting" should be understood in a broad sense, for example, can be fixedly connected, set, or can be detachably connected, set, or integrally connected, set. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0046] Please refer to Figures 1-12 The present application provides a rapid detection kit for Aeromonas, comprising:

[0047] The top base 1 and the bottom base 4 are fixed with an inner shell 11 between them, and the outer wall of the top base 1 is rotatably connected with a ring-shaped cover 2 through a bearing, and the bottom of the ring-shaped cover 2 is fixed with an outer shell 3 which is attached to the outer wall of the inner shell 11;

[0048] The multi-station assembly 12 is arranged in the inner shell 11, the multi-station assembly 12 comprises a rotating disc 121 movably arranged in the inner shell 11, and the outer wall of the rotating disc 121 is provided with equidistantly distributed telescopic grooves 126, the inner wall of each telescopic groove 126 is slidably connected with a telescopic rod 122, one end of the telescopic rod 122 is fixedly connected with a first spring 127 at one end of the telescopic groove 126, and the other end of the telescopic rod 122 is fixedly connected with a supporting cylinder 125, and the projection of the second reagent dropping port 29 and the second observation port 18 covers the center area of two adjacent supporting cylinders 125, respectively, the transparent carrier dish 123 is arranged in each supporting cylinder 125, and the air sac bacterium test paper 124 is arranged on the transparent carrier dish 123, and the clamping assembly for centering and clamping the transparent carrier dish 123 is arranged in the supporting cylinder 125, the clamping assembly comprises an insertion slot arranged on the inner wall of the supporting cylinder 125, and the inner wall of the insertion slot is slidably connected with a clamping pad 128, the inner side surface of the clamping pad 128 is tightly combined with the surface of the transparent carrier dish 123, and the outer end of the clamping pad 128 is fixedly connected with a second spring 129 at one end of the insertion slot, the clamping pad 128 is made of elastic silica gel, and the top seat 1 is provided with a driving mechanism for driving the multi-station assembly 12 to step rotate, the driving mechanism comprises a mounting hole 27 arranged at the center of the top seat 1, and the inner wall of the mounting hole 27 is rotatably connected with a driving shaft 16 through a sealing bearing, the rotating disc 121 is coaxially fixedly connected with the bottom of the driving shaft 16, the top of the top seat 1 is fixedly connected with a stepping motor 9, the output shaft of the stepping motor 9 is connected with the top end of the driving shaft 16 through a shaft coupling, the top of the top seat 1 is provided with an equipment groove 15 on one side, and a controller 8 and a storage battery 28 are fixedly arranged in the equipment groove 15, respectively, the controller 8, the storage battery 28 and the stepping motor 9 are electrically connected, and the opening of the equipment groove 15 is provided with a cover plate, the clamping pad 128 in the clamping assembly cooperates with the elastic force of the second spring 129 to center and clamp the transparent carrier dish 123 from multiple directions, which not only avoids the deviation of the transparent carrier dish 123 in the rotating process to cause inaccurate dropping position, but also prevents the damage of the hard clamping to the transparent carrier dish 123, ensures the consistency of the contact between the sample and the test paper, and utilizes the stepping motor 9 to drive the rotating disc 121 to step rotate through the driving shaft 16, cooperates with the reagent dropping port, the observation port on the top seat 1 and the ring-shaped cover 2, and the taking and placing port on the outer shell 3 and the inner shell 11 to realize the continuous performance of the processes of "dropping-reaction-observation-taking and placing", and a plurality of samples can be processed at a time, compared with the single-channel detection mode, the detection efficiency is improved;

[0049] The drying assembly is arranged in the middle area of the base 4.

[0050] The pressing assembly 17 is partially arranged on the top base 1 and partially extends into the inner shell 11. The pressing assembly 17 comprises a mounting groove 171 arranged on one side of the bottom of the top base 1. The inner wall of the mounting groove 171 is fixedly installed with a gas guide cylinder 172. The middle part of the gas guide cylinder 172 is rotatably connected with a transmission shaft 178. The outer wall of the transmission shaft 178 is fixedly installed with a spiral blade 1711 which is attached to the inner wall of the gas guide cylinder 172. The bottom of the transmission shaft 178 is fixedly installed with a pinion 179. The outer wall of the drive shaft 16 is fixedly installed with a gear wheel 173 which is engaged with the pinion 179. The inner wall of the inner shell 11 and the bottom of the top base 1 are fixedly installed with an annular sealing seat 13 and an arc-shaped sealing seat 176 which are attached to the top surface of the transparent carrier dish 123. The two ends of the annular sealing seat 13 and the two ends of the arc-shaped sealing seat 176 form two work position gaps. The positions of the two work position gaps correspond to the positions of the second reagent dropping port 29 and the second observation port 18 respectively. The inside of the arc-shaped sealing seat 176 is provided with a gas collecting cavity 1710. The bottom of the gas collecting cavity 1710 is provided with a plurality of exhaust holes 177 which are distributed at equal distances. The top of one side of the gas guide cylinder 172 is fixedly installed with an air inlet pipe 174. The bottom of one side of the gas guide cylinder 172 and the gas collecting cavity 1710 are fixedly installed with an exhaust pipe 175. When the drive shaft 16 rotates, the transmission shaft 178 is driven to rotate through the engagement of the gear wheel 173 and the pinion 179. The spiral blade 1711 in the gas guide cylinder 172 generates an air flow. The air flow is introduced into the gas collecting cavity 1710 of the arc-shaped sealing seat 176 through the exhaust pipe 175 and then acts on the test paper in the transparent carrier dish 123 through the exhaust holes 177. Thus, the air flow is used to blow the sample to be detected which is dropped by dripping, so that the sample to be detected is diffused on the aeromonas test paper 124. At the same time, the pressure in the transparent carrier dish 123 is increased, so that the sample to be detected can penetrate into the aeromonas test paper 124 more quickly. The problem of slow sample diffusion in traditional test paper detection is solved, and the requirement of rapid detection is met.

[0051] The ejection assembly 21 is arranged in the inner shell 11.

[0052] The top of the top base 1 is provided with the second reagent dropping port 29 and the second observation port 18 respectively. The top of the annular cover 2 is provided with the first reagent dropping port 7 and the first observation port 6 respectively. The axis of the first reagent dropping port 7 is collinear with the axis of the second reagent dropping port 29. The axis of the first observation port 6 is collinear with the axis of the second observation port 18.

[0053] The cross section of the second observation port 18 is designed in a T shape. The inner wall of the second observation port 18 is fixedly installed with the colorimetric card 26 and the magnifying glass 19 from top to bottom in sequence. The color development area of the aeromonas test paper 124 can be magnified by multiple times through the magnifying glass 19, so that the operator can clearly observe the color change. The colorimetric card 26 can be used to quickly complete the result comparison. The problem of unclear color development and large interpretation error in traditional detection is solved.

[0054] The second taking and placing opening 30 is arranged on one side of the inner shell 11, and the first taking and placing opening 5 is correspondingly arranged on one side of the outer shell 3.

[0055] In the application, the ejection assembly 21 comprises support rods 211 fixed at equal distances on the inner wall of the inner shell 11, and the top of each support rod 211 is fixedly installed with the same ejection disc 212, the ejection disc 212 is composed of a disc part 2121 and an integrally formed protruding part 2122, the position of the protruding part 2122 and the position of the second taking and placing opening 30 are both corresponding to the middle area of the second observation opening 18 and the next support cylinder 125, the bottom of each telescopic rod 122 is rotatably connected with an ejection wheel 213 through a pin shaft, and the ejection wheel 213 slides along the surface of the ejection disc 212, when the rotating disc 121 drives the support cylinder 125 to move to the taking and placing opening position, the ejection wheel 213 at the bottom of the telescopic rod 122 slides along the surface of the protruding part 2122, so that the telescopic rod 122 drives the support cylinder 125 to move to one side, when the maximum displacement is reached, the transparent carrier dish 123 is completely ejected from the taking and placing opening, and the operator can easily take and place without needing to stretch into the inside of the reagent box, so as to facilitate the cleaning of the carrier dish and the test paper, and the operation convenience is improved.

[0056] In the application, the drying assembly comprises a screw hole 32 arranged at the middle of the base 4, and a drying box 23 is screwed on the inner wall of the screw hole 32, the inside of the drying box 23 is filled with a drying agent 24, and a leak-proof mesh plate 25 is fixedly installed on the inner wall top of the drying box 23, so as to prevent the drying agent 24 from scattering and enable the drying agent 24 to effectively absorb the water vapor in the inside of the reagent box, so as to keep the inside dry, avoid the test paper from being invalid due to damp, and prolong the shelf life of the test paper.

[0057] In the application, the top of the top base 1 and the inner wall of the top of the annular cover 2, and the inner wall of the outer shell 3 and the outer wall top and bottom of the inner shell 11 are all arranged with annular sealing grooves 31, and the inner walls of the adjacent two groups of sealing grooves 31 are attached with sealing strips 14, the top of the top base 1 is rotatably connected with a U-shaped handle frame 10 through a pin shaft, and the bottom of the base 4 is fixedly installed with equidistantly distributed suction cups 22, the annular sealing of the top base 1 and the annular cover 2, and the outer shell 3 and the inner shell 11 is realized through the sealing grooves 31 and the sealing strips 14, so as to effectively prevent the test paper from being polluted by bacteria and dust in the external environment, and the handle frame 10 of the top base 1 facilitates the carrying and transportation of the whole reagent box, is suitable for the scene of on-site rapid screening, and meanwhile, the suction cups 22 at the bottom of the base 4 can stably fix the reagent box on a plane such as a table top, so as to avoid the shaking of the reagent box during operation.

[0058] The elastic buckle 20 is arranged between the outer wall of the top seat 1 and the inner wall of the annular cover 2 at equal distances, and the elastic buckle 20 comprises a connecting groove arranged on the outer wall of the top seat 1 and a clamping groove arranged on the inner wall of the annular cover 2, the inner wall of the connecting groove is inserted with an elastic pin rod, one end of the elastic pin rod is fixedly installed with a third spring, the other end of the elastic pin rod is inserted into the clamping groove, and the inner wall of the clamping groove and the other end of the elastic pin rod are both designed as spherical structures, the central angles of the adjacent two elastic buckles 20, the first taking and placing opening 5 and the second taking and placing opening 30, the first observation opening 6 and the second observation opening 18 are all equal, through the design of the elastic buckle 20, the annular cover 2 can be accurately positioned and fixed when rotating, the accurate alignment of various interfaces such as the dropping opening, the observation opening and the taking and placing opening is ensured, and the stability of operation is improved.

[0059] In summary, the working principle of the present application is as follows:

[0060] In use, the operator first rotates the annular cover 2, so that the first reagent dropping opening 7 at the top of the annular cover 2 coincides with the second reagent dropping opening 29 at the top of the top seat 1, the first observation opening 6 at the top of the annular cover 2 coincides with the second observation opening 18 at the top of the top seat 1, and the first taking and placing opening 5 on one side of the outer shell 3 coincides with the second taking and placing opening 30 on one side of the inner shell 11, and the elastic buckle 20 between the outer wall of the top seat 1 and the inner wall of the annular cover 2 is automatically positioned and locked during the rotation process, the elastic pin rod is inserted into the clamping groove under the action of the third spring, and the accurate butt joint and stable fixation of various interfaces are ensured.

[0061] When the detection starts, the sample to be detected is dropped into the corresponding transparent carrier dish 123 below through the aligned first reagent dropping opening 7 and the second reagent dropping opening 29, then the controller 8 in the equipment groove 15 starts the stepping motor 9, the output shaft of the stepping motor 9 drives the driving shaft 16 to rotate through the shaft coupling, the rotating disc 121 at the bottom of the driving shaft 16 is stepped to rotate, the rotating disc 121 drives the support cylinder 125 carrying the transparent carrier dish 123 to rotate synchronously, and the work position switching of sample detection is realized.

[0062] When the driving shaft 16 rotates, the large gear 173 on the outer wall of the driving shaft 16 meshes with the small gear 179 at the bottom of the transmission shaft 178, drives the transmission shaft 178 and the spiral blade 1711 in the air guide cylinder 172 to rotate to generate airflow, the airflow enters the air guide cylinder 172 through the air inlet pipe 174, is guided into the air collecting cavity 1710 of the arc-shaped sealing seat 176 through the air outlet pipe 175, and is finally blown to the transparent carrier dish 123 moving below from the air outlet hole 177 at the bottom of the air collecting cavity 1710, under the blowing and pressure of the airflow, the sample to be detected rapidly diffuses and penetrates the aeromonas test paper 124, and the problem of slow diffusion in traditional detection is solved.

[0063] When the transparent carrier tray 123 carrying the reaction test paper is moved to the position under the second observation port 18, the operator can read the results through the first observation port 6 and the second observation port 18, and the magnifying glass 19 in the second observation port 18 can magnify the color development area of the test paper, and the colorimetric card 26 below can realize accurate comparison;

[0064] After the observation is completed, the rotating disc 121 continues to drive the support cylinder 125 to move to the position of the second taking and placing port 30, at this time, the ejector wheel 213 at the bottom of the telescopic rod 122 slides along the convex part 2122 of the ejector disc 212, pushes the telescopic rod 122 to extend outward along the telescopic groove 126 of the rotating disc 121, and then drives the support cylinder 125 and the transparent carrier tray 123 to be ejected from the first taking and placing port 5 and the second taking and placing port 30, so as to facilitate the operator to take and place the carrier tray and replace the test paper;

[0065] During the whole detection process, the drying agent 24 filled in the drying box 23 can absorb the water vapor inside the inner shell 11 through the leak-proof mesh plate 25, so as to keep the environment dry, the sealing groove 31 and the sealing strip 14 between the top seat 1 and the annular cover 2 and between the outer shell 3 and the inner shell 11 form annular sealing, so as to prevent external pollution, the suction cup 22 at the bottom of the bottom seat 4 can ensure that the reagent box is stably placed, and the handle frame 10 is convenient for equipment transfer, so as to realize continuous, rapid and accurate detection of multiple samples.

[0066] In combination with the actual needs at present, the protection scope of the above-mentioned embodiments of the present application is not limited to this, various changes made within the knowledge range of the persons skilled in the art without departing from the concept of the present application still fall within the protection scope of the present application.

Claims

1. A rapid detection kit for Aeromonas hydrophila, characterized in that, include: Top seat (1) and base (4), with an inner shell (11) fixed between the top seat (1) and the base (4), and an annular cover (2) rotatably connected to the outer wall of the top seat (1) via a bearing, with an outer shell (3) fixed to the bottom of the annular cover (2) and attached to the outer wall of the inner shell (11). Multi-station assembly (12) is disposed inside the inner shell (11), and a drive mechanism for driving the multi-station assembly (12) to rotate in a stepping manner is provided on the top seat (1). A drying assembly is disposed in the middle region of the base (4); A pressure application assembly (17) is partially disposed on the top seat (1) and partially extends into the inner shell (11); Ejection assembly (21), which is disposed within the inner shell (11); The top of the top seat (1) is provided with a second reagent dispensing port (29) and a second observation port (18), and the top of the annular cover (2) is provided with a first reagent dispensing port (7) and a first observation port (6), respectively. The axes of the first reagent dispensing port (7) and the second reagent dispensing port (29) are collinear, and the axes of the first observation port (6) and the second observation port (18) are collinear. The second observation port (18) has a T-shaped cross-section, and its inner wall is fixedly installed with a colorimeter card (26) and a magnifying glass (19) from top to bottom. The inner shell (11) has a second opening (30) on one side, and the outer shell (3) has a first opening (5) on one side. The position of the first opening (5) is adapted to the size of the second opening (30) and the positions are corresponding. The pressure application assembly (17) includes a mounting groove (171) formed on one side of the bottom of the top seat (1), and an air guide cylinder (172) is fixedly installed on the inner wall of the mounting groove (171). A drive shaft (178) is rotatably connected to the middle of the air guide cylinder (172). A spiral blade (1711) is fixedly installed on the outer wall of the drive shaft (178) and fits against the inner wall of the air guide cylinder (172). A small gear (179) is fixedly installed at the bottom of the drive shaft (178), and a large gear (173) is fixedly installed on the outer wall of the drive shaft (16). The large gear (173) meshes with the small gear (179). A fitting is fixedly installed between the top of the inner wall of the inner shell (11) and the bottom of the top seat (1). The top surface of the carrier dish (123) has an annular sealing seat (13) and an arc-shaped sealing seat (176), and the two ends of the annular sealing seat (13) and the two ends of the arc-shaped sealing seat (176) form two work station gaps. The positions of the two work station gaps correspond to the positions of the second reagent drop port (29) and the second observation port (18), respectively. The arc-shaped sealing seat (176) has a gas collecting chamber (1710) inside, and the bottom of the gas collecting chamber (1710) has equidistantly distributed exhaust holes (177). The top of one side of the gas guide tube (172) is fixed with an air inlet pipe (174), and the bottom of one side of the gas guide tube (172) is fixed with an exhaust pipe (175) between the bottom of one side of the gas guide tube (172) and the gas collecting chamber (1710).

2. The Aeromonas rapid detection kit according to claim 1, characterized in that, The multi-station component (12) includes a rotating disk (121) movably disposed within the inner shell (11), and the outer wall of the rotating disk (121) is provided with telescopic grooves (126) distributed at equal intervals. Telescopic rods (122) are slidably inserted into the inner wall of each telescopic groove (126). A first spring (127) is fixedly installed at one end of the telescopic rod (122) and at one end of the telescopic groove (126). A support cylinder (125) is fixedly installed at the other end of each telescopic rod (122). The projections of the second reagent dispensing port (29) and the second observation port (18) respectively cover the central area of ​​two adjacent support cylinders (125). A transparent carrier dish (123) is placed inside each support cylinder (125), and Aeromonas test paper (124) is laid inside each transparent carrier dish (123). A clamping component for centering and clamping the transparent carrier dish (123) is provided inside the support cylinder (125).

3. The Aeromonas rapid detection kit according to claim 2, characterized in that, The clamping assembly includes slots evenly spaced on the inner wall of the support cylinder (125), and clamping pads (128) are slidably inserted into the inner wall of each slot. The inner side of the clamping pads (128) is tightly attached to the surface of the transparent carrier dish (123). A second spring (129) is fixedly installed at the outer end of the clamping pads (128) and one end of the slot. The clamping pads (128) are made of elastic silicone material.

4. The Aeromonas rapid detection kit according to claim 3, characterized in that, The drive mechanism includes a mounting hole (27) at the center of the top seat (1), and the inner wall of the mounting hole (27) is rotatably connected to the drive shaft (16) through a sealed bearing. The rotating disk (121) is coaxially fixedly installed at the bottom of the drive shaft (16), and a stepper motor (9) is fixedly installed at the top of the top seat (1). The output shaft of the stepper motor (9) is connected to the top of the drive shaft (16) through a coupling.

5. The Aeromonas rapid detection kit according to claim 4, characterized in that, The ejection assembly (21) includes support rods (211) fixed at equal intervals to the inner wall of the inner shell (11), and the same ejection plate (212) is fixedly installed on the top of the support rods (211). The ejection plate (212) consists of a disc part (2121) and an integrally formed protrusion part (2122). The position of the protrusion part (2122) and the position of the second pick-up and drop-out port (30) correspond to the middle area between the second observation port (18) and the next support cylinder (125). The bottom of the telescopic rod (122) is rotatably connected to the ejection wheel (213) through a pin, and the ejection wheel (213) slides along the surface of the ejection plate (212).

6. The Aeromonas rapid detection kit according to claim 5, characterized in that, The drying assembly includes a screw hole (32) in the middle of the base (4), and a drying box (23) is screwed into the inner wall of the screw hole (32). The drying box (23) is filled with desiccant (24), and a leak-proof mesh plate (25) is fixedly installed on the top of the inner wall of the drying box (23).

7. The Aeromonas rapid detection kit according to claim 6, characterized in that, A ring-shaped sealing groove (31) is provided between the top of the top seat (1) and the top inner wall of the annular cover (2), and between the inner wall of the outer shell (3) and the top and bottom of the outer wall of the inner shell (11). A sealing strip (14) is attached to the inner wall of two adjacent sets of sealing grooves (31). A U-shaped handle frame (10) is rotatably connected to the top of the top seat (1) through a pin. Suction cups (22) are fixedly installed at equal distances at the bottom of the base (4).

8. The Aeromonas rapid detection kit according to claim 7, characterized in that, The outer wall of the top seat (1) and the inner wall of the annular cover (2) are provided with elastic buckles (20) distributed at equal intervals. The elastic buckles (20) include a connecting groove on the outer wall of the top seat (1) and a slot on the inner wall of the annular cover (2). An elastic pin is inserted into the inner wall of the connecting groove. A third spring is fixedly installed at one end of the elastic pin and one end of the connecting groove. The other end of the elastic pin is inserted into the slot. The inner wall of the slot and the other end of the elastic pin are both designed as spherical structures. The central angle distance between two adjacent elastic buckles (20), the first take-out port (5) and the second take-out port (30), and the first observation port (6) and the second observation port (18) are all equal.

9. The Aeromonas rapid detection kit according to claim 8, characterized in that, The top of the top seat (1) has an equipment slot (15) on one side, and a controller (8) and a battery (28) are fixedly installed in the equipment slot (15). The controller (8), the battery (28) and the stepper motor (9) are electrically connected. The opening of the equipment slot (15) is equipped with a cover plate.

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