Full-process automatic all-in-one machine for DNA extraction, amplification and sequencing
By employing a negative pressure environment, a cooling base, and an air suction plate in the fully automated DNA extraction, amplification, and sequencing integrated machine, the problem of contaminant leakage in PCR experiments has been solved, thereby improving the safety and accuracy of biological experiments.
Patent Information
- Application Number
- CN202410873166.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2026-01-06
AI Technical Summary
Biological experiments, especially PCR experiments, are prone to generating pollutants such as aerosols, resulting in low pollution and safety during the experimental process. Furthermore, these pollutants can easily escape into the environment, affecting the safety of the environment and personnel.
A fully automated integrated machine for DNA extraction, amplification, and sequencing was designed. It adopts a negative pressure environment, a cooling base for the amplification area, and an air suction plate. It is equipped with three layers of protection against contaminant leakage, including a negative pressure system inside the chamber, a connection between the air suction plate and the air outlet, and a filter in the air inlet, to ensure that contaminants are discharged in a timely manner.
It improves the safety and accuracy of biological experiments, effectively reduces the leakage of pollutants through three protective measures, and ensures the stability and safety of the experimental environment.
Smart Images
Figure CN121271684A_ABST
Abstract
Description
Technical Field
[0001] This application belongs to the field of biosafety technology, and in particular relates to an integrated machine for the entire process of DNA extraction, amplification and sequencing. Background Technology
[0002] In biological experiments, especially PCR (gene amplification) experiments, aerosols and other pollutants are easily generated, which can contaminate samples during the experiment and affect the smooth progress of the biological experiment. Furthermore, during the loading process in the operating chamber, these pollutants can easily escape, causing pollution to personnel and the environment, resulting in lower safety levels in the testing operation. Summary of the Invention
[0003] This application provides an integrated automated machine for the entire process of DNA extraction, amplification, and sequencing, which addresses the technical problem of how to provide a cabin that improves the accuracy and safety of biological experiments.
[0004] In a first aspect, this application provides an automated integrated machine for the entire DNA extraction, amplification, and sequencing process, comprising a chamber; the chamber contains a first space, within which a biological sample extraction workbench is provided for realizing the entire DNA extraction, amplification, and sequencing process; the top of the chamber is provided with an air inlet duct, and the sides and / or bottom of the chamber are provided with air outlet ducts, the air inlet duct and the air outlet duct forming a negative pressure within the chamber; the biological sample extraction workbench is provided with an amplification zone, the amplification zone is provided with a cooling base for reducing contaminants generated during the amplification process, and the sides of the amplification zone are provided with an air suction plate; the air suction plate is connected to the air outlet duct, discharging contaminants generated in the amplification zone through the air outlet duct from the chamber.
[0005] In one implementation of the first aspect, a PCR plate is provided in the amplification area, and the suction plates are disposed on both sides of the PCR plate.
[0006] In one implementation of the first aspect, the suction plate has suction holes on its inner surface facing the PCR plate, and the suction holes are connected to suction pipes located inside the suction plate. Pollutants generated in the amplification area enter the suction pipes through the suction holes.
[0007] In one implementation of the first aspect, the air inlet duct includes an air inlet filter, through which external air is filtered before entering the cabin.
[0008] In one implementation of the first aspect, a sample storage unit is provided in the second space, and the sample storage unit is fixed to the boundary between the first space and the second space by screws.
[0009] In one implementation of the first aspect, the sample storage unit includes a stage and a guide rail structure, wherein the stage moves within the first space and the second space via the guide rail structure located underneath.
[0010] In one implementation of the first aspect, the fully automated integrated machine for DNA extraction, amplification, and sequencing further includes a support frame, the cabin is mounted on the support frame, and casters are installed at the bottom of the support frame.
[0011] In one implementation of the first aspect, a centrifuge is provided on the biological sample extraction workbench, and the centrifuge is fixedly installed on the biological sample extraction workbench.
[0012] In one implementation of the first aspect, the biological sample extraction workbench is provided with a clamp, which moves the clamped sample along a first direction and / or a second direction via a moving device located above the chamber.
[0013] In one implementation of the first aspect, the moving device includes a motor, a first moving unit, and a second moving unit, wherein the clamp is assembled with the first moving unit to move the clamp along a first direction, and the first moving unit and the second moving unit are assembled perpendicularly to move the first moving unit along a second direction.
[0014] The fully automated integrated machine for DNA extraction, amplification, and sequencing described in this application has the following advantages: This application employs a three-tiered protection system against contaminant leakage through a negative pressure environment, a cooling base in the amplification zone, and a suction plate, further enhancing the safety and accuracy of biological experiments. Simultaneously, this application provides movable and extendable clamps to meet the customized layout requirements of the workbench. Attached Figure Description
[0015] Figure 1 The image shown is a schematic diagram of the appearance of an integrated machine for fully automated DNA extraction, amplification, and sequencing, as described in one embodiment of this application.
[0016] Figure 2 The diagram shown is a structural schematic of an integrated machine for fully automated DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0017] Figure 3 The diagram shown is a structural schematic of an integrated machine for fully automated DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0018] Figure 4 The diagram shown is a schematic diagram of the internal structure of an integrated automated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0019] Figure 5The diagram shown is a schematic diagram of the internal structure of an integrated automated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0020] Figure 6 The diagram shown is a schematic diagram of the internal structure of an integrated automated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0021] Figure 7 The diagram shown is a schematic diagram of the second spatial structure of an integrated machine for fully automated DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0022] Figure 8 The diagram shown is a schematic diagram of the second spatial structure of an integrated machine for fully automated DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0023] Figure 9 The diagram shown is a schematic diagram of the second spatial structure of an integrated machine for fully automated DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0024] Figure 10 The diagram shown is a schematic representation of the sample storage unit of an automated integrated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0025] Figure 11 The diagram shown is a schematic representation of the sample storage unit of an automated integrated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0026] Figure 12 The diagram shown is an installation schematic of a centrifuge for a fully automated integrated machine for DNA extraction, amplification, and sequencing, as described in one embodiment of this application.
[0027] Figure 13 The diagram shown is an installation schematic of a centrifuge for a fully automated integrated machine for DNA extraction, amplification, and sequencing, as described in one embodiment of this application.
[0028] Figure 14 The diagram shown is a schematic of a sample separation device for a fully automated integrated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0029] Figure 15 The diagram shown is an assembly schematic of the fixture and moving device of an automated integrated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0030] Figure 16 The diagram shown is an assembly schematic of the fixture and moving device of an automated integrated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0031] Figure 17 The diagram shown is an assembly schematic of the fixture and moving device of an automated integrated machine for DNA extraction, amplification, and sequencing, as described in an embodiment of this application.
[0032] Component designation explanation
[0033] A cabin
[0034] 1. Biological sample extraction workbench
[0035] 11 Centrifuge
[0036] 2 Cooling base
[0037] 3. Suction plate
[0038] 31 air intake holes
[0039] 4 PCR plates
[0040] 5. Biological sample testing equipment
[0041] 51 Sample storage unit
[0042] 511 Platform
[0043] 512 guide rail structure
[0044] 6. Fixtures
[0045] 61 Gripper
[0046] 62 and telescopic pole
[0047] 7. Mobile devices
[0048] 71 Motor
[0049] 72 First Moving Unit
[0050] 73 Second Moving Unit
[0051] 8. Sample separation device
[0052] B support frame
[0053] B1 Universal Wheel Detailed Implementation
[0054] The following specific examples illustrate the implementation of this application. Those skilled in the art can easily understand other advantages and effects of this application from the content disclosed in this specification. This application can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this application. It should be noted that, unless otherwise specified, the following embodiments and features in the embodiments can be combined with each other.
[0055] It should be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of this application. Therefore, the drawings only show the components related to this application and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the form, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.
[0056] Furthermore, the use of terms such as "first" and "second" in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. If the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed in this application.
[0057] In biological experiments, especially PCR (polymerase chain reaction) experiments, aerosols and other contaminants are easily generated. Current technologies cannot effectively prevent the leakage of contaminants, thus adversely affecting the accuracy and safety of the experiment.
[0058] To at least address the aforementioned issues, this application provides an automated integrated machine for the entire DNA extraction, amplification, and sequencing process. By implementing three layers of protection against contaminant leakage, it further enhances the safety and accuracy of biological experiments.
[0059] Please see Figures 1 to 3 As shown in the figure, an embodiment of this application provides an automated integrated machine for the entire process of DNA extraction, amplification, and sequencing, including a chamber A; the chamber A has a first space, and the first space has a biological sample extraction workbench 1 for realizing the entire process of DNA extraction, amplification, and sequencing; the top of the chamber A has an air inlet duct (not shown), and the side and / or bottom of the chamber A have air outlet ducts (not shown), and the air inlet duct and the air outlet duct form a negative pressure in the chamber; the biological sample extraction workbench 1 has an amplification zone, and the amplification zone has a cooling base 2 for reducing contaminants generated in the amplification process, and the side of the amplification zone has an air suction plate 3; the air suction plate 3 is connected to the air outlet duct, and discharges the contaminants generated in the amplification zone into the chamber A through the air outlet duct.
[0060] It should be noted that the biological sample extraction workbench can automatically complete the entire process of DNA extraction, amplification, and sequencing under the control of control commands, without the need for human intervention or assistance in any part of the process.
[0061] It should be noted that the negative pressure formed inside chamber A means that the air pressure inside chamber A is greater than the air pressure outside. This prevents pollutants generated inside chamber A from easily escaping through gaps or when the doors are opened, thus forming the first line of defense for biological experiments. In some embodiments, the air inlet duct may include an intake fan to bring outside air into chamber A, and the air outlet duct may include an exhaust fan to exhaust air from chamber A to the outside.
[0062] In some embodiments, a pressure sensor may also be installed inside chamber A. The pressure sensor is communicatively connected to a PLC controller, which in turn is communicatively connected to an intake fan and an exhaust fan. When the pressure sensor detects that the pressure inside chamber A does not meet the negative pressure condition, the intake fan or exhaust fan can be controlled to operate until the pressure inside chamber A reaches the set requirement. The PLC controller then controls the intake fan or exhaust fan to stop operating or operate at a set power, thereby meeting the set negative pressure condition and ensuring a stable experimental environment inside chamber A.
[0063] It should be noted that the cooling base 2 can cool biological samples. In PCR, biological samples are often amplified by heating, which generates a large amount of contaminants. The cooling base allows for rapid cooling of biological samples, reducing experimental time and minimizing non-specific amplification that may occur at intermediate temperatures. It also effectively reduces contaminant generation, thus forming a second line of defense in biological experiments.
[0064] For details, please continue reading. Figures 4 to 6 The amplification area is equipped with a PCR plate 4, and the suction plate 3 is disposed on both sides of the PCR plate 4.
[0065] Specifically, the suction plate 3 has suction holes 31 on its inner surface facing the PCR plate 4. These suction holes 31 are connected to a suction duct (not shown) located inside the suction plate. Pollutants generated in the amplification area enter the suction duct through the suction holes 31. In this way, by placing the suction plate 3 on both sides of the PCR plate 4, aerosols and other pollutants generated during PCR amplification can be promptly drawn in, effectively preventing contamination of the chamber environment and the risk of potential leakage, thus forming the third line of defense for biological experiments.
[0066] In some embodiments, the outlet air of the air outlet duct is equipped with an outlet one-way valve, which is used to restrict the flow of outside gas into the cabin through the air outlet duct, thereby contaminating the biological samples inside the cabin.
[0067] Specifically, the air intake duct includes an air intake filter. Outside air enters the chamber A after being filtered by the air intake filter, in order to prevent pollutants in the outside air from contaminating the environment inside the chamber A, and to further improve the accuracy and safety of biological experiments.
[0068] For details, please refer to Figures 7 to 9 The cabin contains a second space, which contains a biological sample testing device 5 and a sample storage unit 51. Figure 10 and Figure 11 The specific structural design of sample storage unit 51 is shown below. Figure 10 and Figure 11 The sample storage unit 51 includes a stage 511 and a guide rail structure 512. The stage 511 is used to hold biological samples. The stage 511 can move within the first space and the second space via the guide rail 512, so as to move the stage 511 to the testing area for testing the biological samples. Further, as... Figure 10 As shown, the sample storage unit 51 also includes a mounting frame, which is fixed at the junction of the first space and the second space by screws.
[0069] For details, please continue reading. Figure 1 and Figure 2 The fully automated integrated machine for DNA extraction, amplification, and sequencing provided in this application embodiment also includes a support frame B. The chamber A is mounted on the support frame B, and casters B1 are installed at the bottom of the support frame B. In some embodiments, the support frame B is a frame structure made of multiple aluminum profiles. The air outlet at the bottom of the chamber A can be set in the gaps of the frame. This allows for maintenance of the air outlet through the gaps when needed, while also making full use of the frame gaps without occupying other extra space in the testing room, thus improving space utilization. In this case, contaminants generated inside the chamber A can be directly discharged downwards from the chamber A through the air outlet at the bottom of the air outlet. In other embodiments, the support frame B can also be a cabinet with an internal hollow layer, and the air outlet at the bottom of the chamber A can be set in the hollow layer of the support frame B to discharge contaminants from the chamber A.
[0070] It should be noted that the air inlet of the air inlet duct and the air outlet of the air outlet duct of the chamber A both extend to the outside of the chamber A, thereby preventing the air inside the chamber A from communicating with the outside air and improving the safety and accuracy of biological experiments.
[0071] In some embodiments, the fixing part of the universal wheel B1 can be welded to the support frame B. The universal wheel B1 enables free movement of the fully automated DNA extraction, amplification, and sequencing integrated machine. Thus, the fully automated DNA extraction, amplification, and sequencing integrated machine provided in this application is not only small in size but also capable of automatic movement, thereby meeting various experimental environments and needs.
[0072] For details, please refer to Figure 12 and Figure 13 As shown, a centrifuge 11 is installed on the biological sample extraction workbench 1. The centrifuge 11 is fixedly installed on the biological sample extraction workbench 1 to receive biological samples to be separated. This application optimizes the layout of the biological sample extraction workbench by installing large equipment such as centrifuges on the operating table, thereby further reducing the required area of the chamber. The centrifuge can be used to separate and extract biological samples. After the biological samples are separated by the centrifuge 11, the sample plate containing the biological samples is separated by the sample separation device 8.
[0073] For further details, please refer to Figure 14 The sample separation device 8 is used to separate the sample plate (microplate). In biological experiments, biological samples are often extracted using tools such as cotton swabs and stored in the sample plate. The sample plate provided in this embodiment is divided into upper and lower tube racks. The test tubes on the upper rack are shorter and used to store extraction tools such as cotton swabs, while the test tubes on the lower rack are longer and used to store biological samples. The test tube openings on the two racks correspond to each other; when the two racks are connected, the test tubes on the upper rack are inserted into the corresponding test tubes on the lower rack. After the sample plate is separated by the centrifuge 11, it is further separated by the sample separation device 8, thus retaining only the biological samples in the test tubes of the lower rack for subsequent experimental work. In some embodiments, the sample separation device 8 can separate the sample plate by pressing down and pushing up.
[0074] For details, please refer to Figures 15 to 17 As shown, the biological sample extraction workbench 1 is equipped with a clamp 6. The clamp 6 moves the clamped sample along a first direction and / or a second direction via a moving device 7 located above the chamber A. Depending on the different needs of biological experiments, different experimental equipment is often installed on the biological sample extraction workbench 1 for extracting biological samples. In this case, it is necessary to provide the clamp 6 to allow the biological sample to move freely, so that it can be placed on different experimental equipment.
[0075] For details, please continue reading. Figures 15 to 17The moving device 7 includes a first moving unit 72 and a second moving unit 73. The clamp 6 is assembled with the first moving unit 72 to move the clamp 6 along a first direction. The first moving unit 72 and the second moving unit 73 are assembled perpendicularly to move the first moving unit 72 along a second direction.
[0076] In some embodiments, a reference coordinate system is set in the fully automated integrated machine for DNA extraction, amplification, and sequencing. The first direction is the X direction and the second direction is the Y direction. That is, the clamp 6 can move along the X direction through the first moving unit 72, and at the same time, the first moving unit 72 can move along the Y direction through the second moving unit 73. This enables the clamp 6 to move simultaneously or separately along the X and Y directions, thereby enabling the delivery of biological samples into different spatial regions.
[0077] For details, please continue reading. Figure 16 The clamp 6 includes a gripper 61 and a telescopic rod 62. One end of the telescopic rod 62 is fixedly connected to the gripper 61, and the other end is connected to the motor. In some embodiments, a reference coordinate system is set within the fully automated DNA extraction, amplification, and sequencing integrated machine, and the extension direction of the telescopic rod 62 is the Y direction. Since the heights of the installed experimental equipment vary, the height of the gripper 61 can be automatically adjusted via the telescopic rod 62 to achieve precise sample gripping and movement.
[0078] In some embodiments, the integrated automated DNA extraction, amplification, and sequencing system provided in this application can be equipped with a biological sample extraction workbench, centrifuge, cooling base, moving device, clamps, sample separation device, pipette, purification device, and biological sample testing equipment to realize the biological sample detection process. All of the above experimental devices can be installed within the chamber; this application does not impose any restrictions on the specific equipment layout and selection. For an example of the above experimental equipment, please refer to [further details]. Figures 1 to 17 The biological experimental process of the fully automated integrated machine for DNA extraction, amplification, and sequencing provided in this application includes:
[0079] First, reagent preparation is performed. In the first space, the sample is dispensed using a pipette. Then, the biological sample is extracted using a cotton swab and placed in a sample plate, which consists of two layers of tube racks. The tubes on the upper rack are shorter, and the tubes on the lower rack are longer. The tube openings of the two racks correspond; when the two racks are connected, the tubes on the upper rack are inserted into the corresponding tubes on the lower rack, thus completing sample preparation. The sample plate is then held by clamps and can be moved in the X or Y direction using a moving device. The moving device sends the sample plate into a centrifuge for cell lysis and separation, splashing the liquid from the tube walls and caps to the bottom of the tubes on the lower rack. Afterwards, any cotton swabs or other tools remaining in the upper rack are discarded. The clamps continue to hold the biological sample in the tubes on the lower rack for purification, cooling, and PCR processing. Finally, the PCR amplification products are sent to the sample storage unit of the biological sample testing device in the second space, and then moved to the testing area via the sample storage unit to achieve amplification and analysis.
[0080] Furthermore, the fully automated integrated machine for DNA extraction, amplification, and sequencing provided in this application also includes a coating device within its chamber. In some embodiments, the coating device coats the sample plates. The coating device includes a film-applying block and suction holes located on the lower surface of the film-applying block, the suction holes being arranged in a rectangular array on the film-applying block. Simultaneously, a pipe connector is provided on the upper surface of the film-applying block, which connects to a corresponding air extraction unit. When needed, a negative pressure can be created at the suction holes to adsorb the sealing film. Specifically, the coating device draws in the sealing film through the suction holes, and after the film-applying block presses the sealing film onto the corresponding sample plate, the negative pressure at the suction holes is eliminated to release the sealing film, thus sealing the sealing film onto the corresponding sample plate and completing the coating operation.
[0081] In some embodiments, the laminating device is connected to a lifting mechanism, which can be fixedly connected to the moving device. This allows the moving device to be moved to a designated area while reducing the volume, and then the laminating module is lowered to a set height by the lifting mechanism to complete the corresponding film suction or pressing action.
[0082] For details, please continue reading. Figure 1 The chamber A is also equipped with a display device 8. The display device 8 includes a screen, which can display the detection results of biological samples inside chamber A. The experimental results can be automatically read and retained by an automated program, eliminating the need for manual recording and further reducing experimental costs. Furthermore, the experimental environment inside chamber A can be directly controlled via the display device 8 without requiring manual adjustment inside the chamber, achieving comprehensive automated control. This application is not limited to the specific type of display device 8.
[0083] In some embodiments, the top of chamber A is further equipped with ultraviolet disinfection equipment, monitoring equipment, and lighting equipment to further improve the disinfection and monitoring of the experimental environment inside the chamber and enhance the safety of biological experiments. This application is not limited to the specific types and models of the ultraviolet disinfection equipment, monitoring equipment, and lighting equipment.
[0084] As described above, the fully automated DNA extraction, amplification, and sequencing integrated machine provided in this application employs a three-tiered protection system against contaminant leakage through a negative pressure environment, a pressure-reducing base in the amplification zone, and a suction plate, further enhancing the safety and accuracy of biological experiments. Simultaneously, this application provides movable and extendable clamps to meet the customized layout requirements of the workbench.
[0085] The descriptions of the processes or structures corresponding to the above figures each have their own emphasis. For parts of a process or structure that are not described in detail, please refer to the relevant descriptions of other processes or structures.
[0086] The above embodiments are merely illustrative of the principles and effects of this application and are not intended to limit this application. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this application. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this application should still be covered by the claims of this application.
Claims
1. A DNA extraction amplification sequencing full-process automation all-in-one machine, characterized in that, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 2.The DNA extraction, amplification and sequencing all-process automation integrated machine according to claim 1, characterized in that, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 3.The DNA extraction, amplification and sequencing all-process automation integrated machine according to claim 2, characterized in that, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 4.The DNA extraction, amplification and sequencing all-process automation integrated machine of claim 1, wherein, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 5.The DNA extraction, amplification and sequencing all-process automation integrated machine of claim 1, wherein, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 6.The DNA extraction, amplification and sequencing all-process automation integrated machine of claim 5, characterized in that, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 7.The DNA extraction, amplification and sequencing all-process automation integrated machine according to claim 1, characterized in that, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 8.The DNA extraction, amplification and sequencing all-process automation integrated machine of claim 1, wherein, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 9.The DNA extraction, amplification and sequencing all-in-one machine of claim 1, wherein, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 10.The DNA extraction, amplification and sequencing all-in-one machine of claim 8, wherein, The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. 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The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing a whole process of DNA extraction, amplification and sequencing. The cabin body is internally provided with a first space, and the first space is internally provided with a