Preparation device and process of wheat germ fermentation liquor
By designing a motor-driven placement plate and sampling plate structure, the problems of poor oxygen transmission and temperature and humidity changes in the culture medium in wheat germ fermentation equipment were solved, thereby improving the fermentation effect and stabilizing the temperature and humidity.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- XIAMEN YANGZHEN HEALTH FOOD CO LTD
- Filing Date
- 2025-12-09
- Publication Date
- 2026-04-17
AI Technical Summary
In existing fermentation equipment, the culture medium is in a static state during wheat germ fermentation, oxygen transmission is poor, the growth of the strain is limited, and the temperature and humidity changes caused by opening the door to take samples affect the fermentation effect.
A wheat germ fermentation broth preparation device was designed. The placement plate and sampling plate structure driven by a motor make the culture medium reciprocate and vibrate in the vertical direction. Combined with the design of electromagnet and magnetic spring, uniform contact of the culture medium and oxygen transfer are achieved, and the temperature and humidity inside the constant temperature chamber are kept stable during sampling.
It improves fermentation efficiency, promotes microbial growth and metabolism, ensures stable temperature and humidity during fermentation, and avoids the impact of temperature and humidity changes on subsequent fermentation.
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Figure CN121271693B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of fermentation equipment technology, specifically to a device and process for preparing wheat germ fermentation broth. Background Technology
[0002] Wheat germ is a byproduct of wheat milling. A wheat grain consists of three parts: bran, endosperm, and germ, with the germ accounting for approximately 2% to 3% of the grain's weight. Wheat germ is extremely rich in high-quality protein, fat, various vitamins, minerals, and trace bioactive components. It possesses anti-aging, antioxidant, anti-tumor, cholesterol-lowering, and triglyceride-lowering functions, earning it the reputation of a "natural nutritional treasure trove for humankind." In recent years, domestic and international research on wheat germ has shifted from the extraction of its main components to biotransformation. This involves utilizing one or more special enzymes produced by microorganisms to convert certain inherent substances in wheat germ into active substances or reduce its anti-nutritional factors, thereby achieving structural modification of its functional components and improving its nutritional and functional properties. Currently, the production of deep-processed wheat germ products in China is still in its infancy and has not yet reached a large scale. Furthermore, there is limited research on using microbial fermentation methods to improve the antioxidant activity of wheat germ.
[0003] Fermentation equipment is often used in the fermentation process of wheat germ. However, when fermenting wheat germ and culture medium made from microorganisms, the culture medium is in a static state, which is not conducive to oxygen transfer and microbial growth. Furthermore, due to different amounts of microorganisms added or differences in temperature and humidity within the fermentation equipment, the culture medium needs to be removed sequentially for observation and testing. However, when the fermentation equipment is opened to remove the medium, the temperature and humidity inside the fermentation device will change too much, affecting the subsequent fermentation effect. Summary of the Invention
[0004] The purpose of this invention is to solve the problems existing in the prior art by proposing a preparation device and process for wheat germ fermentation broth.
[0005] To achieve the above objectives, the present invention adopts the following technical solution:
[0006] A device for preparing wheat germ fermentation broth includes a constant temperature chamber and a placement plate. An L-shaped plate is fixedly connected to the upper side wall of the constant temperature chamber. A through hole is opened in the bottom wall of the L-shaped plate. A second motor is fixedly connected to the side wall of the L-shaped plate. A rotating shaft is fixedly connected to the output end of the second motor. An extension rod is symmetrically fixedly connected to the side wall of the rotating shaft. A connecting rod is rotatably connected to the other end of the extension rod. Two cylinders are rotatably connected to the side wall of the L-shaped plate. A first connecting rod is fixedly connected to the side wall of the cylinders. The other end of the first connecting rod is rotatably connected to the connecting rod. A round pin is symmetrically rotatably connected to the side wall of the L-shaped plate. A second connecting rod is rotatably connected to the side wall of the round pin. An L-shaped rod is fixedly connected to the side wall of the cylinder. A sampling plate is rotatably connected to the L-shaped rod and the adjacent second connecting rod.
[0007] Preferably, the top wall of the placement plate is symmetrically provided with conveying grooves, the inner side wall of the conveying groove is fixedly connected with a vertical plate, the side wall of the vertical plate is elastically connected with a pusher plate by a magnetic spring, the pusher plate is slidably connected inside the conveying groove, and the inner side wall of the conveying groove is symmetrically provided with multiple placement grooves.
[0008] Preferably, the sidewall of the placement plate has symmetrical rectangular cavities, and the inner wall of the rectangular cavity is elastically connected to a sliding plug by a return spring. The sliding plug is slidably and sealingly connected to the inner wall of the rectangular cavity, and a wedge-shaped rod is fixedly connected to the sidewall of the sliding plug.
[0009] Preferably, the bottom wall of the push plate has a slot, and the inner wall of the slot is elastically connected to a locking block by a tension spring. The top wall of the placement plate has symmetrical insertion holes, and the locking block is inserted into the insertion hole. The insertion hole is sealed and slidably connected to a sliding plate. The rectangular cavity is fixedly connected to the inner wall of the insertion hole by a connecting pipe.
[0010] Preferably, a partition is slidably connected to the inner side wall of the constant temperature chamber, a back plate is installed on the top of the partition, a slide rail is fixedly connected to the side wall of the back plate, a connecting column is slidably connected to the inner wall of the slide rail, a base plate is fixedly connected to the top of the connecting column, and the top of the base plate is slidably connected to the placement plate.
[0011] Preferably, a first motor is fixedly connected to the side wall of the back plate, a drive shaft is fixedly connected to the output end of the first motor, a circular plate is fixedly connected to the side wall of the drive shaft, and a rectangular frame is fixedly connected to the bottom end of the connecting column, the rectangular frame being fitted and sliding on the side of the circular plate.
[0012] Preferably, a crossbar is fixedly connected to one end of the drive shaft, a rotating rod is rotatably connected to the side wall of the back plate, a swing rod is fixedly connected to the side wall of the rotating rod, the top end of the swing rod is rotatably connected to the side wall of the placement plate through a first rod, and the bottom end of the swing rod is rotatably connected to the crossbar through a second rod.
[0013] Preferably, a T-shaped rod is slidably connected through the inner wall of the placement groove, a plurality of electromagnets are symmetrically fixedly connected to the side wall of the placement plate, and a permanent magnet is fixedly connected to the side wall of the T-shaped rod.
[0014] Preferably, the L-shaped plate has two limiting posts fixedly connected to its side wall, and one of the extension rods abuts against one of the limiting posts after moving a certain distance. The sampling plate covers the inside of the through hole, and a flow groove is provided on the top wall of the sampling plate. An electric push rod is fixedly connected to the bottom inner wall of the constant temperature chamber, and the movable end of the electric push rod is fixedly connected to the partition. A sampling chamber door is installed on the top wall of the constant temperature chamber.
[0015] A process for preparing wheat germ fermentation broth includes the following steps:
[0016] S1. Weighing: Weigh a certain amount of wheat germ powder;
[0017] S2. Preparation of wheat germ culture medium: Wheat germ powder and water are mixed in a weight-to-volume ratio of 1:6 to 1:12 to prepare wheat germ fermentation medium. A pH adjuster is added to adjust the pH to 6.0-7.0. The medium is then sterilized in an autoclave at 121°C for 20 minutes.
[0018] S3. Fermentation: Add the inoculum at 0.8%-1.2% of the weight of the wheat germ fermentation medium. The inoculum is composed of Lactobacillus acidophilus and Bacillus subtilis in a 1:2 ratio. Shake on a constant temperature shaker for 20 minutes to ensure that the wheat germ liquid is dispersed as much as possible and does not clump. Then, carry out fermentation culture in a constant temperature incubator at 35℃-40℃. After fermentation for 24h-64h, take it out and centrifuge at 3000r / min for 40 minutes in a low speed centrifuge. Take the supernatant to obtain the wheat germ fermentation liquid.
[0019] Compared with existing technologies, the advantages of this invention are:
[0020] 1. Turn on the first motor. By setting up a rectangular frame, crossbar, and placement plate, the multiple culture media placed on the placement plate reciprocate vertically. The culture media on the placement plate can also vibrate continuously, so that the culture media and biological strains can fully contact each other, improve the fermentation effect, and also improve the uniformity of the inoculated strains in the culture environment and oxygen transfer, which helps the growth and metabolism of the inoculated strains and can accelerate the growth and metabolic process of the biological strains.
[0021] 2. When the fermentation status in the culture medium needs to be tested after a period of fermentation, an electromagnet installed on the side wall of the placement plate is turned on, so that the T-shaped rod pushes the corresponding culture medium from the placement tank into the conveying tank during the movement. Then, the second motor is turned on, so that each sampling plate moves horizontally downward at a certain angle, ready to take out the selected culture medium.
[0022] 3. Activate the electric push rod, and then rotate the output end of the second motor in the opposite direction by a certain angle. By setting up structures such as wedge rods, connecting pipes, and sliding plates, the locking block is pushed upward and disengaged from the insertion hole. At this time, under the elastic force of the magnetic spring, the magnetic spring will extend from the compressed state. The magnetic spring will then drive the push plate to slide along the conveying groove. The push plate will then synchronously transport the culture medium that has entered the conveying groove to the flow groove of the corresponding sampling plate, thus completing the sampling of the culture medium.
[0023] 4. The second motor continues to rotate in the opposite direction, causing the two sampling plates to be reinserted into the through holes. At this point, the two sampling plates seal the through holes, preventing excessive temperature and humidity changes inside the incubator when the culture medium is removed through the sampling box door, which would affect the subsequent culture and fermentation effect of the culture medium. Attached Figure Description
[0024] Figure 1 This is a schematic diagram of the structure of a wheat germ fermentation broth preparation device proposed in this invention;
[0025] Figure 2 This is a schematic diagram showing the positional relationship between the conveying tank and the placement tank in a wheat germ fermentation broth preparation device proposed in this invention;
[0026] Figure 3 for Figure 2 Enlarged schematic diagram of section B of the structure;
[0027] Figure 4 This is a schematic diagram of the internal structure of the rectangular cavity and the insertion hole in the wheat germ fermentation broth preparation device proposed in this invention;
[0028] Figure 5 for Figure 4 Enlarged schematic diagram of section A of the structure;
[0029] Figure 6 This is a schematic diagram showing the connection relationship of the extension rod, L-shaped rod, and second connecting rod in the wheat germ fermentation broth preparation device proposed in this invention.
[0030] Figure 7 This is a schematic diagram showing the installation position of the first motor in the wheat germ fermentation broth preparation device proposed in this invention.
[0031] Figure 8 This is a schematic diagram showing the connection relationship between the slide rail and the back plate in a wheat germ fermentation broth preparation device proposed in this invention.
[0032] In the diagram: 1. Constant temperature chamber; 2. Partition; 3. Electric push rod; 4. Back plate; 5. First motor; 6. Drive shaft; 7. Crossbar; 8. Second rod; 9. Rotating rod; 10. Swinging rod; 11. First rod; 12. Rectangular frame; 13. Slide rail; 14. Connecting column; 15. Base plate; 16. Placement plate; 17. Placement slot; 18. T-shaped rod; 19. Conveying slot; 20. Push plate; 21. Magnetic spring; 22. Vertical plate; 23. L-shaped plate; 24. Rotating shaft; 25. Extension rod; 26. 27. Cylinder; 28. First connecting rod; 29. Connecting rod; 30. L-shaped rod; 31. Round pin; 32. Second connecting rod; 33. Through hole; 34. Sampling plate; 35. Flow groove; 36. Second motor; 37. Permanent magnet; 38. Electromagnet; 39. Rectangular cavity; 40. Return spring; 41. Sliding plug; 42. Wedge rod; 43. Empty groove; 44. Tension spring; 45. Locking block; 46. Insertion hole; 47. Slide plate; 48. Connecting pipe; 49. Round plate; 50. Sampling box door; 51. Limiting post. Detailed Implementation
[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0034] Reference Figure 1 - Figure 8A device for preparing wheat germ fermentation broth includes a constant temperature chamber 1 and a placement plate 16. The constant temperature chamber 1 is prior art and will not be described in detail here. An L-shaped plate 23 is fixedly connected to the upper side wall of the constant temperature chamber 1. A through hole 32 is opened on the bottom wall of the L-shaped plate 23. A second motor 35 is fixedly connected to the side wall of the L-shaped plate 23. The second motor 35 is a forward and reverse reversible motor. A rotating shaft 24 is fixedly connected to the output end of the second motor 35. An extension rod 25 is symmetrically fixedly connected to the side wall of the rotating shaft 24. A connecting rod 28 is rotatably connected to the other end of the extension rod 25. Two cylinders 26 are rotatably connected to the side wall of the L-shaped plate 23. A first connecting rod 27 is fixedly connected to the side wall of the cylinders 26. The other end of the first connecting rod 27 is connected to an adjacent cylinder. The connecting rod 28 is rotatably connected, and the side wall of the L-shaped plate 23 is symmetrically rotatably connected to the pin 30. The side wall of the pin 30 is rotatably connected to the second connecting rod 31. The side wall of the cylinder 26 is fixedly connected to the L-shaped rod 29. The L-shaped rod 29 and the adjacent second connecting rod 31 are rotatably connected to the sampling plate 33. The rotating connection parts of the L-shaped rod 29 and the adjacent second connecting rod 31 and the sampling plate 33 are at different positions, so that the sampling plate 33 is always in a horizontal state when making arc-shaped movements. On the one hand, it can facilitate subsequent contact with the wedge rod 41 (the specific principle will be explained later) to generate compression and trigger subsequent operations when moving downward in an arc. On the other hand, it can stably sample the culture medium when moving upward in an arc.
[0035] The top wall of the placement plate 16 is symmetrically provided with conveying grooves 19. A vertical plate 22 is fixedly connected to the inner side wall of the conveying groove 19. A pusher plate 20 is elastically connected to the side wall of the vertical plate 22 through a magnetic spring 21. The magnetic spring 21 is existing technology. After the magnetic spring 21 is energized, due to electromagnetic induction, the magnetic spring 21 interacts with the current in the external magnetic field to generate different magnitudes of tension to control the stretching deformation and movement state of the magnetic spring 21. That is, the magnetic spring 21 can contract after being energized. After the magnetic spring 21 drives the corresponding pusher plate 20 to slide a certain distance, the magnetic spring 21 can be energized to drive the pusher plate 20 to slide and reset in the opposite direction. The pusher plate 20 is slidably connected inside the conveying groove 19. Multiple placement grooves 17 are symmetrically provided on the inner side wall of the conveying groove 19.
[0036] The sidewalls of the placement plate 16 are symmetrically provided with rectangular cavities 38 (e.g. Figure 4 As shown), a return spring 39 is fixedly connected to the inner wall of the rectangular cavity 38. A slide plug 40 is fixedly connected to the other end of the return spring 39. The slide plug 40 is slidably connected to the inner side wall of the rectangular cavity 38. A wedge rod 41 is fixedly connected to the side wall of the slide plug 40.
[0037] The bottom wall of the push plate 20 has a hollow groove 42 (e.g. Figure 5 As shown), the inner wall of the slot 42 is elastically connected to a locking block 44 by a tension spring 43, and the top wall of the placement plate 16 is symmetrically provided with insertion holes 45 (as shown). Figure 4 and Figure 5As shown), the card block 44 is inserted into the socket 45. The socket 45 is sealed and slidably connected to the slide plate 46. The rectangular cavity 38 is fixedly connected to the inner wall of the socket 45 through the connecting pipe 47. The connecting pipe 47 is located on the inner wall of the rectangular cavity 38 near the reset spring 39.
[0038] The inner wall of the constant temperature chamber 1 is slidably connected to a partition 2, a back plate 4 is installed on the top of the partition 2, a slide rail 13 is fixedly connected to the side wall of the back plate 4, a connecting column 14 is slidably connected to the inner wall of the slide rail 13, a bottom plate 15 is fixedly connected to the top of the connecting column 14, and the top of the bottom plate 15 is slidably connected to the placement plate 16.
[0039] The first motor 5 is fixedly connected to the side wall of the back plate 4 (e.g. Figure 7 As shown), the output end of the first motor 5 is fixedly connected to the drive shaft 6, the side wall of the drive shaft 6 is eccentrically fixedly connected to the circular plate 48, and the bottom end of the connecting column 14 is fixedly connected to the rectangular frame 12, which is fitted and slidably on the side of the circular plate 48.
[0040] A crossbar 7 is fixedly connected to one end of the drive shaft 6, a rotating rod 9 is rotatably connected to the side wall of the back plate 4, a swing rod 10 is fixedly connected to the side wall of the rotating rod 9, the top end of the swing rod 10 is rotatably connected to the side wall of the placement plate 16 through the first rod 11, and the bottom end of the swing rod 10 is rotatably connected to the crossbar 7 through the second rod 8.
[0041] A T-shaped rod 18 is slidably connected through the inner wall of the placement slot 17, and multiple electromagnets 37 are symmetrically fixedly connected to the side wall of the placement plate 16. A permanent magnet 36 is fixedly connected to the side wall of the T-shaped rod 18.
[0042] Two limiting posts 50 are fixedly connected to the side wall of the L-shaped plate 23. After moving a certain distance, the two extension rods 25 each come into contact with a limiting post 50. The sampling plate 33 covers the inside of the through hole 32. A flow groove 34 is opened on the top wall of the sampling plate 33. An electric push rod 3 is fixedly connected to the bottom inner wall of the constant temperature chamber 1. The movable end of the electric push rod 3 is fixedly connected to the partition plate 2. A sampling chamber door 49 is installed on the top wall of the constant temperature chamber 1.
[0043] A process for preparing wheat germ fermentation broth includes the following steps:
[0044] S1. Weighing: Weigh a certain amount of wheat germ powder;
[0045] S2. Preparation of wheat germ culture medium: Wheat germ powder and water are mixed in a weight-to-volume ratio of 1:6 to 1:12 to prepare wheat germ fermentation medium. A pH adjuster is added to adjust the pH to 6.0-7.0. The medium is then sterilized in an autoclave at 121°C for 20 minutes.
[0046] S3. Fermentation: Add the inoculum at 0.8%-1.2% of the weight of the wheat germ fermentation medium. The inoculum is composed of Lactobacillus acidophilus and Bacillus subtilis in a 1:2 ratio. Shake on a constant temperature shaker for 20 minutes to ensure that the wheat germ liquid is dispersed as much as possible and does not clump. Then, carry out fermentation culture in a constant temperature incubator at 35℃-40℃. After fermentation for 24h-64h, take it out and centrifuge at 3000r / min for 40 minutes in a low speed centrifuge. Take the supernatant to obtain the wheat germ fermentation liquid.
[0047] In this invention, multiple culture media composed of wheat germ fermentation broth inoculated with bacterial strains are placed in multiple placement tanks 17 on a placement plate 16 for constant temperature fermentation.
[0048] When the first motor 5 is turned on, its output drives the drive shaft 6 to rotate. The drive shaft 6 then drives the circular plate 48, which is fixedly connected to its side wall, to rotate eccentrically. During the rotation, the circular plate 48 will cause the rectangular frame 12 and the connecting column 14, which are fitted and slide together with it, to reciprocate vertically. The connecting column 14, in turn, causes the base plate 15 and the placement plate 16 to reciprocate vertically in sync, resulting in the multiple culture media placed on the placement plate 16 reciprocating vertically. Furthermore, the drive shaft 6 will also drive the second rod 8, which is rotatably connected to it, to move via the crossbar 7. The second rod 8 will then... The swing rod 10, which is rotatably connected to it, rotates a certain angle clockwise and counterclockwise around the rotating rod 9. This causes the swing rod 10 to drive the placement plate 16 to slide horizontally back and forth a short distance on the base plate 15 via the first rod 11. This continuous vibration of the culture medium on the placement plate 16 ensures thorough contact between the culture medium and the microbial inoculum, improving the fermentation effect. Furthermore, it enhances the uniformity of the inoculum in the culture environment and improves oxygen transport, thus promoting the growth and metabolism of the inoculum and accelerating the growth and metabolic processes of the microbial inoculum.
[0049] When fermentation needs to be monitored after a period of time, several selected culture media from the constant temperature chamber 1 are removed for testing. The first motor 5 is controlled to position the placement plate 16 in the center. Then, an electromagnet 37 installed on the side wall of the placement plate 16 is activated. The energized electromagnet 37 and the adjacent permanent magnet 36, with opposite polarities, attract each other. Under this magnetic attraction, the permanent magnet 36 drives the T-shaped rod 18 to move a distance, pushing the corresponding culture media from the placement tank 17 into the conveying tank 19. Next, the second motor 35 is activated. The second motor 35 is a forward and reverse rotating motor, and its output drives... When the rotating shaft 24 rotates, the extension rod 25, which is symmetrically fixed to the side wall of the rotating shaft 24, will drive the connecting rod 28, which is rotatably connected to it, to move a certain distance toward the second motor 35. Then, each connecting rod 28 will drive the first connecting rod 27, which is rotatably connected to it, to move. This causes the first connecting rod 27 to drive the cylinder 26, which is fixedly connected to it, to rotate a certain angle. Then, the cylinder 26 will drive the L-shaped rod 29, which is fixedly connected to it, to rotate a certain angle. With the cooperation of the second connecting rod 31, the sampling plate 33, which is rotatably connected to the L-shaped rod 29 and the second connecting rod 31, moves obliquely downward in a horizontal state at a certain angle. During this process, the two sampling plates 33 move away from each other, and the movement trajectory is an arc structure.
[0050] When the electric push rod 3 is activated, its movable end causes the partition 2 to move upward a certain distance, causing the placement plate 16 to move upward synchronously along with the two wedge-shaped rods 41 symmetrically arranged on its sidewall. Then, the output end of the second motor 35 is rotated in the opposite direction by a certain angle, causing the two sampling plates 33 to move in the opposite direction a certain distance until the sidewall of the sampling plate 33 and the adjacent wedge-shaped rod 41 come into contact and are compressed. Under this compressive force, the wedge-shaped rod 41 will cause the fixedly connected sliding plug 40 to slide a certain distance, thereby allowing the mineral inside the rectangular cavity 38 to be transported through the connecting pipe 47. When the culture medium is squeezed into the socket 45, the sliding plate 46, which is sealed and slidably connected inside the socket 45, will slide upwards a certain distance. During the upward sliding process, the sliding plate 46 will push the locking block 44 upwards until it is disengaged from the socket 45. At this time, under the elastic force of the magnetic spring 21, the magnetic spring 21 will extend from the compressed state. The magnetic spring 21 will then drive the push plate 20 to slide along the conveying groove 19. The push plate 20 will then synchronously convey the culture medium that has entered the conveying groove 19 to the flow groove 34 of the corresponding sampling plate 33, thus completing the sampling of the culture medium.
[0051] Next, the movable end of the electric push rod 3 is moved downward, causing the wedge rod 41 and the sampling plate 33 to disengage. Then, the second motor 35 is rotated in the opposite direction, causing the two sampling plates 33 to be reinserted into the through hole 32. At this time, the two sampling plates 33 seal the through hole 32, preventing excessive changes in temperature and humidity inside the constant temperature chamber 1 when the culture medium is taken out through the sampling box door 49, which would affect the subsequent culture and fermentation effect of the culture medium.
[0052] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.
Claims
1. A device for preparing wheat germ fermentation broth, comprising a constant temperature chamber (1) and a placement plate (16), characterized in that, An L-shaped plate (23) is fixedly connected to the upper side wall of the constant temperature chamber (1). A through hole (32) is opened on the bottom wall of the L-shaped plate (23). A second motor (35) is fixedly connected to the side wall of the L-shaped plate (23). A rotating shaft (24) is fixedly connected to the output end of the second motor (35). An extension rod (25) is symmetrically fixedly connected to the side wall of the rotating shaft (24). A connecting rod (28) is rotatably connected to the other end of the extension rod (25). Two connecting rods (28) are rotatably connected to the side wall of the L-shaped plate (23). A cylinder (26) is fixedly connected to a first connecting rod (27) on its side wall. The other end of the first connecting rod (27) is rotatably connected to a connecting rod (28). A round pin (30) is symmetrically rotatably connected to the side wall of the L-shaped plate (23). A second connecting rod (31) is rotatably connected to the side wall of the round pin (30). An L-shaped rod (29) is fixedly connected to the side wall of the cylinder (26). The L-shaped rod (29) and the adjacent second connecting rod (31) are rotatably connected to a sampling plate (33). The top wall of the placement plate (16) is symmetrically provided with a conveying groove (19). A vertical plate (22) is fixedly connected to the inner side wall of the conveying groove (19). A pusher plate (20) is elastically connected to the side wall of the vertical plate (22) through a magnetic spring (21). The pusher plate (20) is slidably connected inside the conveying groove (19). A plurality of placement grooves (17) are symmetrically provided on the inner side wall of the conveying groove (19). The placement plate (16) has rectangular cavities (38) symmetrically opened on the side wall. The inner wall of the rectangular cavity (38) is elastically connected to a sliding plug (40) by a return spring (39). The sliding plug (40) is slidably connected to the inner side wall of the rectangular cavity (38). A wedge rod (41) is fixedly connected to the side wall of the sliding plug (40). The bottom wall of the push plate (20) is provided with a slot (42), and the inner wall of the slot (42) is elastically connected with a locking block (44) by a tension spring (43). The top wall of the placement plate (16) is symmetrically provided with insertion holes (45), and the locking block (44) is inserted into the insertion hole (45). The insertion hole (45) is sealed and slidably connected with a sliding plate (46). The rectangular cavity (38) is fixedly connected to the inner wall of the insertion hole (45) through a connecting pipe (47). The inner wall of the constant temperature chamber (1) is slidably connected to a partition (2), a back plate (4) is installed on the top of the partition (2), a slide rail (13) is fixedly connected to the side wall of the back plate (4), a connecting column (14) is slidably connected to the inner wall of the slide rail (13), a bottom plate (15) is fixedly connected to the top of the connecting column (14), and the top of the bottom plate (15) is slidably connected to the placement plate (16). The inner wall of the placement groove (17) is slidably connected to a T-shaped rod (18), and multiple electromagnets (37) are symmetrically fixedly connected to the side wall of the placement plate (16). A permanent magnet (36) is fixedly connected to the side wall of the T-shaped rod (18). The L-shaped plate (23) has two limiting posts (50) fixedly connected to its side wall. One of the extension rods (25) comes into contact with one of the limiting posts (50) after moving a certain distance. The sampling plate (33) covers the inside of the through hole (32). The top wall of the sampling plate (33) is provided with a flow groove (34). The bottom inner wall of the constant temperature box (1) is fixedly connected with an electric push rod (3). The movable end of the electric push rod (3) is fixedly connected to the partition (2). The top wall of the constant temperature box (1) is equipped with a sampling box door (49).
2. The apparatus for preparing wheat germ fermentation broth according to claim 1, characterized in that, The back plate (4) is fixedly connected to the side wall of the first motor (5), the output end of the first motor (5) is fixedly connected to the drive shaft (6), the side wall of the drive shaft (6) is eccentrically fixedly connected to the circular plate (48), the bottom end of the connecting column (14) is fixedly connected to the rectangular frame (12), and the rectangular frame (12) is sleeved and slid on the side of the circular plate (48).
3. The apparatus for preparing wheat germ fermentation broth according to claim 2, characterized in that, A crossbar (7) is fixedly connected to one end of the drive shaft (6), a rotating rod (9) is rotatably connected to the side wall of the back plate (4), a swing rod (10) is fixedly connected to the side wall of the rotating rod (9), the top end of the swing rod (10) is rotatably connected to the side wall of the placement plate (16) through a first rod (11), and the bottom end of the swing rod (10) is rotatably connected to the crossbar (7) through a second rod (8).
4. A process for preparing wheat germ fermentation broth, comprising using the wheat germ fermentation broth preparation apparatus according to any one of claims 1-3, characterized in that, It also includes the following steps: S1. Weighing: Weigh the wheat germ powder; S2. Preparation of wheat germ culture medium: Wheat germ powder and water are mixed in a weight-to-volume ratio of 1:6 to 1:12 to prepare wheat germ fermentation medium. A pH adjuster is added to adjust the pH to 6.0-7.
0. The medium is then sterilized in an autoclave at 121°C for 20 minutes. S3. Fermentation: Add the inoculum at 0.8%-1.2% of the weight of the wheat germ fermentation medium. The inoculum is composed of Lactobacillus acidophilus and Bacillus subtilis in a 1:2 ratio. Shake on a constant temperature shaker for 20 minutes to ensure that the wheat germ liquid is dispersed and does not clump. Then, carry out fermentation culture in a constant temperature incubator at 35℃-40℃. After fermentation for 24-64 hours, take it out and centrifuge at 3000r / min for 40 minutes in a low speed centrifuge. Take the supernatant to obtain the wheat germ fermentation liquid.
Citation Information
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