Infant formula milk powder

Abstract

The invention belongs to the technical field of food, and particularly relates to infant formula milk powder. Compared with the prior art, the infant milk powder provided by the invention can promote the growth and development of infants, improve the comfort level of intestinal tracts, reduce the index score of feeding difficulty, promote the abundance of bifidobacterium and bifidobacterium breve in the intestinal tracts of the infants, remarkably increase the richness and species number of flora in the intestinal tracts of the infants, and improve the immunity of the infants. And a flora state closer to that of breast feeding is formed.

Description

Technical Field

[0001] This invention belongs to the field of food technology, and in particular relates to an infant formula milk powder. Background Technology

[0002] During an infant's growth and development, the trends in height and weight gain are important indicators of health and nutritional status. When a baby's height increases significantly over a period of time while their weight maintains a steady increase (rather than excessively rapid growth), this is actually a very healthy and ideal growth pattern with unique advantages. This is because it means the baby is prioritizing the building of lean body mass (such as muscle, bones, and organs) rather than excessively accumulating fat. This lays a healthier metabolic foundation for the child, helping to reduce the risk of future obesity and related metabolic diseases (such as childhood diabetes). Summary of the Invention

[0003] In view of this, the technical problem to be solved by the present invention is to provide an infant formula milk powder that improves the intestinal comfort and flora distribution of infants and provides better feeding results.

[0004] This invention provides an infant formula milk powder, which, per 100 g of infant formula milk powder, comprises: 500-2000 mg of minerals, 20-500 mg of polyunsaturated fatty acids, 1-24 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100 mg of nucleotides and 200-600 mg of lactoferrin.

[0005] The minerals include 2-6 mg of iron, 1-5 mg of zinc, and 300-500 mg of calcium.

[0006] Preferably, per 100 g of infant formula milk powder, it includes: 500-2000 mg of minerals, 20-500 mg of polyunsaturated fatty acids, 1-24 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100 mg of nucleotides and 360-600 mg of lactoferrin.

[0007] The minerals include 4-6 mg of iron, 3-4 mg of zinc, and 300-400 mg of calcium;

[0008] Preferably, per 100 g of infant formula milk powder, it includes: 500-2000 mg of minerals, 20-500 mg of polyunsaturated fatty acids, 1-10 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100 mg of nucleotides and 470-540 mg of lactoferrin.

[0009] The minerals include 4.75 mg of iron, 3.77 mg of zinc, and 350 mg of calcium.

[0010] Preferably, per 100 g of formula milk powder, the infant formula milk powder further includes 200-500 mg of vitamins;

[0011] And / or, per 100 g of infant formula, the infant formula also includes 10-200 mg of vitamin-like substances;

[0012] And / or, per 100 g of infant formula, the infant formula may further include 0.5 to 2 g of functional oligosaccharides.

[0013] Preferably, the polyunsaturated fatty acids include docosahexaenoic acid (DHA) and eicosatraenoic acid (EPA); the mass ratio of DHA to EPA is 1:(0.8~1.5); preferably, the mass ratio of DHA to EPA is 1:(0.9~1.1).

[0014] The vitamin-like substance includes choline and taurine; the mass ratio of choline to taurine is (1~5):1; preferably, the mass ratio of choline to taurine is (3~3.5):1.

[0015] The functional oligosaccharides include galactooligosaccharides and fructooligosaccharides; the mass ratio of galactooligosaccharides to fructooligosaccharides is (1~6):1, preferably, the mass ratio of galactooligosaccharides to fructooligosaccharides is (3~4.5):1.

[0016] Preferably, the vitamins, calculated per 100 g of formula milk powder, include:

[0017] Vitamin A 300~400 μg RE;

[0018] Vitamin D 10~15 μg;

[0019] Vitamin E 1~15 mg α-TE;

[0020] Vitamin K1 10~150 μg;

[0021] Vitamin B1 300~800 μg;

[0022] Vitamin B2 400~800 μg;

[0023] Vitamin B6 200~600 μg

[0024] Vitamin B 12 0.5~10 μg;

[0025] Nicotinic acid and / or nicotinamide 2000~6000 μg;

[0026] Folic acid 30~100 μg;

[0027] Pantothenic acid 1500~3500 μg;

[0028] Vitamin C 10~100 mg;

[0029] Biotin 10~25 μg;

[0030] And / or, the minerals include:

[0031] Sodium 100~300 mg;

[0032] Potassium 200-600 mg;

[0033] Copper 100~400 μg;

[0034] Magnesium 10~50 mg;

[0035] Iron 4~6 mg;

[0036] Zinc 3~4 mg;

[0037] Manganese 5~60 μg;

[0038] Calcium 300-400 mg;

[0039] Phosphorus 100~300 mg;

[0040] Iodine 1~100 μg;

[0041] Chlorine 100~400 mg;

[0042] Selenium 10~30 μg.

[0043] Preferably, per 100 g of infant formula milk powder, the infant formula milk powder includes 10-23 g of protein, 15-29 g of fat, 2-5 g of linoleic acid, 200-600 mg of α-linolenic acid, and 50-58 g of carbohydrates.

[0044] Preferably, per 100 g of infant formula milk powder, the infant formula milk powder includes 10-15 g of protein, 25-29 g of fat, 4-5 g of linoleic acid, 400-450 mg of α-linolenic acid, and 55-56 g of carbohydrates.

[0045] Preferably, the protein sources in the infant formula milk powder include one or more of cow's milk, whole milk powder, skim milk powder, demineralized whey powder and whey protein powder, α-lactalbumin powder and β-casein powder;

[0046] The fat sources in the infant formula include animal fats, vegetable oils, and 1,3-dioleoyl-2-palmitoylglycerol triglycerides; the animal fats include milk fat and / or anhydrous butter; the vegetable oils include one or more of sunflower oil, corn oil, soybean oil, coconut oil, low-erucic acid rapeseed oil, and flaxseed oil.

[0047] And / or, per 100 g of infant formula, the raw materials of the infant formula also include 1-35 g of lactose;

[0048] And / or, per 100 g of infant formula, the raw materials of the infant formula also include 0.01~0.25 g of probiotics; preferably, the probiotics include Bifidobacterium.

[0049] Preferably, per 100g of infant formula, the ingredients include:

[0050] Raw milk 10~200 g;

[0051] Lactose 1~35 g;

[0052] Whole milk powder 0~30 g;

[0053] Skim milk powder 0~32.5 g;

[0054] 10-45 g of demineralized whey powder;

[0055] Whey protein powder 0.1~17 g;

[0056] Sunflower seed oil 1~10 g;

[0057] Corn oil 0.1~4 g;

[0058] Soybean oil 0.5~10 g;

[0059] Coconut oil 0~3 g;

[0060] Low erucic acid rapeseed oil 0~8.5 g;

[0061] Flaxseed oil 0~2 g;

[0062] 1,3-Dioleoyl-2-palmitoylglycerol triglyceride 1~24 g;

[0063] α-Whey protein powder 0.5~5.5 g;

[0064] β-casein powder 0.5~4 g;

[0065] Soybean lecithin 0.1~0.5 g;

[0066] Anhydrous butter 0~0.2 g;

[0067] 0.1~2.5 g of fructooligosaccharide powder;

[0068] 0.1-10 g of galactooligosaccharide syrup;

[0069] 0.1~2 g of docosahexaenoic acid;

[0070] Eicosatraenoic acid 0.1~2.5 g;

[0071] Bifidobacteria 0~0.25 g;

[0072] Lactoferrin 0.2~0.6 g;

[0073] Nucleotides 0.01~0.1 g;

[0074] 0.7~2.5 g of a complex of nutrients containing vitamins, vitamin-like substances and minerals;

[0075] Preferably, the compound nutrients include:

[0076] Vitamin A: 385 μg RE;

[0077] Vitamin D: 12.9 μg;

[0078] Vitamin E: 7 mg α-TE;

[0079] Vitamin K1: 70 μg;

[0080] Vitamin B1: 550 μg;

[0081] Vitamin B2: 600 μg;

[0082] Vitamin B6: 420 μg;

[0083] Vitamin B 12 2.6 μg;

[0084] Nicotinic acid and / or nicotinamide: 4000 μg;

[0085] Folic acid: 79 μg;

[0086] Pantothenic acid: 2850 μg;

[0087] Vitamin C: 65 mg;

[0088] Biotin: 16 μg;

[0089] Choline: 130 mg;

[0090] Taurine: 40 mg;

[0091] Sodium: 190 mg;

[0092] Potassium: 420 mg;

[0093] Copper: 386 μg;

[0094] Magnesium: 32 mg;

[0095] Iron: 4.75 mg;

[0096] Zinc: 3.77 mg;

[0097] Manganese: 50 μg;

[0098] Calcium: 350 mg;

[0099] Phosphorus: 220 mg

[0100] Iodine: 98 μg;

[0101] Chlorine: 325 mg

[0102] Selenium: 19.5 μg.

[0103] Preferably, the infant formula milk powder improves intestinal comfort by promoting the growth of healthy intestinal flora.

[0104] This invention provides an infant formula milk powder, comprising, per 100 g of infant formula milk powder: 500-2000 mg of minerals, 20-500 mg of polyunsaturated fatty acids, 1-24 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100 mg of nucleotides, and 200-600 mg of lactoferrin; the minerals include 2-6 mg of iron, 1-5 mg of zinc, and 300-500 mg of calcium. Compared with the prior art, the infant formula milk powder provided by this invention can promote infant growth and development, improve intestinal comfort, reduce feeding difficulty index scores, promote the abundance of Bifidobacterium and Bifidobacterium breve in the infant gut, and significantly increase the richness and species number of the infant gut microbiota, creating a microbiota state closer to that of breastfed infants. Attached Figure Description

[0105] Figure 1 This is a distribution chart of infant growth and development indicators before propensity score matching in an embodiment of the present invention;

[0106] Figure 2 This is a distribution chart of infant growth and development indicators after propensity score matching in an embodiment of the present invention;

[0107] Figure 3 This is a graph showing the detection results of phylum-level microbiota in infant fecal samples in an embodiment of the present invention;

[0108] Figure 4 This is a heatmap of species abundance at the phylum level in infant fecal samples in an embodiment of the present invention.

[0109] Figure 5 This is a graph showing the detection results of horizontal bacterial communities in infant fecal samples in an embodiment of the present invention;

[0110] Figure 6 This is a heatmap of species abundance of horizontal bacterial communities in infant fecal samples in an embodiment of the present invention;

[0111] Figure 7 This is a graph showing the detection results of microbial communities at different levels in infant fecal samples in an embodiment of the present invention;

[0112] Figure 8 This is a heatmap of species abundance of horizontal microbiota in infant fecal samples in an embodiment of the present invention;

[0113] Figure 9 This is a box plot of feeding difficulty scores for infants in different feeding method groups before and after matching in an embodiment of the present invention;

[0114] Figure 10 This is a bar chart showing the distribution of infant bowel movements before matching in an embodiment of the present invention;

[0115] Figure 11 This is a bar chart showing the distribution of infant bowel movements after matching in an embodiment of the present invention. Detailed Implementation

[0116] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0117] Height growth primarily depends on the effective absorption of sufficient nutrients for bone building, such as protein, calcium, phosphorus, and vitamin D. Stable weight indicates that total energy intake is adequate and appropriate, without excess. This suggests that the baby's diet is of high quality, with balanced nutrient distribution, and that energy is efficiently used for body structure building, rather than simply being converted into fat for storage.

[0118] Furthermore, an infant's growth is not a uniform, linear process, but rather occurs in a step-like or leaping manner. Sometimes, the body will "grow taller" first, followed by weight gain. This pattern of height growth preceding weight gain precisely matches the natural and healthy growth rhythm of many infants.

[0119] Based on this, the present invention provides an infant formula milk powder, which, per 100 g of infant formula milk powder, comprises: 500-2000 mg of minerals, 20-500 mg of polyunsaturated fatty acids, 1-24 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100 mg of nucleotides and 200-600 mg of lactoferrin; wherein the minerals include 2-6 mg of iron, 1-5 mg of zinc and 300-500 mg of calcium.

[0120] The infant formula provided by this invention contains lactoferrin, an important immunologically active protein in breast milk. It can directly deprive bacteria of iron ions required for growth, thereby effectively inhibiting the proliferation of harmful pathogens (such as Escherichia coli and Staphylococcus aureus). Simultaneously, it promotes the development of the infant's own immune system, establishing a strong intestinal and systemic immune barrier, creating a safe and stable internal environment for the baby's growth and development, and reducing the interference of diseases on the growth process. Furthermore, lactoferrin can highly bind to iron, forming an easily absorbed complex, significantly improving iron bioavailability and effectively preventing iron-deficiency anemia. Sufficient iron is crucial for the development of the infant's nervous system and systemic oxygen supply. In addition, it can also promote the absorption of other minerals in the intestines, providing a solid material basis for bone growth (increased height); furthermore, lactoferrin can bind to cell receptors, positively affecting cell growth and differentiation, and may directly participate in and promote intestinal development, bone growth, and brain development.

[0121] This invention provides a method for compounding lactoferrin with other components in formula milk powder in a specific ratio, so that the formula milk powder can promote the growth and development of infants and stabilize their weight gain.

[0122] In one specific embodiment of the present invention, the mineral content in the infant formula milk powder may optionally be 500 mg, 600 mg, 700 mg, 800 mg, 900 mg, 1000 mg, 1100 mg, 1200 mg, 1300 mg, 1400 mg, 1500 mg, 1600 mg, 1700 mg, 1800 mg, 1900 mg, 2000 mg, or any two of the above values, calculated per 100 g of infant formula milk powder.

[0123] In one specific embodiment of the present invention, the minerals may optionally include iron in the form of 2 mg, 2.5 mg, 3 mg, 3.5 mg, 4 mg, 4.5 mg, 4.75 mg, 5 mg, 5.5 mg, 6 mg or any two of the above values, per 100 g of infant formula.

[0124] In one specific embodiment of the present invention, the mineral may optionally include zinc at a concentration of 1 mg, 1.5 mg, 2 mg, 2.5 mg, 3 mg, 3.5 mg, 3.77 mg, 4 mg, 4.5 mg, 5 mg or any two of the above values, per 100 g of infant formula.

[0125] In one specific embodiment of the present invention, the minerals may optionally include calcium at a concentration of 300 mg, 320 mg, 350 mg, 380 mg, 400 mg, 420 mg, 450 mg, 480 mg, 500 mg or any two of the above values, per 100 g of infant formula.

[0126] In one specific embodiment of the present invention, the minerals per 100 g of infant formula milk powder include 4-6 mg of iron, 3-4 mg of zinc and 300-400 mg of calcium; preferably, the minerals include 4.75 mg of iron, 3.77 mg of zinc and 350 mg of calcium.

[0127] In one specific embodiment of the present invention, the minerals, per 100 g of infant formula milk powder, include: 100-300 mg of sodium; 200-600 mg of potassium; 100-400 μg of copper; 10-50 mg of magnesium; 4-6 mg of iron; 3-4 mg of zinc; 5-60 μg of manganese; 300-400 mg of calcium; 100-300 mg of phosphorus; 1-100 μg of iodine; 100-400 mg of chlorine; and 10-30 μg of selenium.

[0128] In one specific embodiment of the present invention, the minerals, optionally, include sodium in the form of 100 mg, 120 mg, 140 mg, 150 mg, 160 mg, 180 mg, 190 mg, 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, or any two of the above values.

[0129] In one specific embodiment of the present invention, the minerals, optionally, per 100 g of infant formula milk powder, include potassium in the range of 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, 320 mg, 340 mg, 350 mg, 360 mg, 380 mg, 390 mg, 400 mg, 420 mg, 440 mg, 450 mg, 460 mg, 480 mg, 490 mg, 500 mg, 520 mg, 540 mg, 550 mg, 560 mg, 580 mg, 590 mg, 600 mg, or any two of the above values.

[0130] In one specific embodiment of the present invention, the minerals, optionally, include copper element in the range of 100 μg, 120 μg, 140 μg, 150 μg, 160 μg, 180 μg, 190 μg, 200 μg, 220 μg, 240 μg, 250 μg, 260 μg, 280 μg, 290 μg, 300 μg, 320 μg, 340 μg, 350 μg, 360 μg, 380 μg, 386 μg, 390 μg, 400 μg, or any two of the above values, per 100 g of infant formula milk powder.

[0131] In one specific embodiment of the present invention, the minerals, optionally, per 100 g of infant formula milk powder, include magnesium in the range of 10 mg, 12 mg, 14 mg, 15 mg, 16 mg, 18 mg, 19 mg, 20 mg, 22 mg, 24 mg, 25 mg, 26 mg, 28 mg, 29 mg, 30 mg, 32 mg, 34 mg, 35 mg, 36 mg, 38 mg, 39 mg, 40 mg, 42 mg, 44 mg, 45 mg, 46 mg, 48 mg, 49 mg, 50 mg, or any two of the above values.

[0132] In one specific embodiment of the present invention, the mineral may optionally include manganese element in the range of 5 μg, 10 μg, 15 μg, 20 μg, 25 μg, 30 μg, 35 μg, 40 μg, 45 μg, 50 μg, 55 μg, 60 μg or any two of the above values, per 100 g of infant formula milk powder.

[0133] In one specific embodiment of the present invention, the minerals, optionally, per 100 g of infant formula milk powder, include phosphorus in the range of 100 mg, 120 mg, 140 mg, 150 mg, 160 mg, 180 mg, 190 mg, 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, or any two of the above values.

[0134] In one specific embodiment of the present invention, the mineral, calculated per 100 g of infant formula milk powder, optionally includes iodine element in the range of 5 μg, 10 μg, 15 μg, 20 μg, 25 μg, 30 μg, 35 μg, 40 μg, 45 μg, 50 μg, 55 μg, 60 μg, 65 μg, 70 μg, 75 μg, 80 μg, 85 μg, 90 μg, 95 μg, 98 μg, 100 μg, or any two of the above values.

[0135] In one specific embodiment of the present invention, the minerals, optionally, per 100 g of infant formula milk powder, include chlorine in the range of 100 mg, 120 mg, 140 mg, 150 mg, 160 mg, 180 mg, 190 mg, 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, 320 mg, 325 mg, 340 mg, 350 mg, 360 mg, 380 mg, 390 mg, 400 mg, or any two of the above values.

[0136] In one specific embodiment of the present invention, the mineral may optionally include 10 μg, 12 μg, 14 μg, 15 μg, 16 μg, 18 μg, 19.5 μg, 20 μg, 22 μg, 24 μg, 25 μg, 26 μg, 28 μg, 30 μg of selenium per 100 g of infant formula, or any range between two of the above values.

[0137] In one specific embodiment of the present invention, the infant formula milk powder, calculated per 100 g, includes polyunsaturated fatty acids in the range of 20 mg, 40 mg, 50 mg, 60 mg, 80 mg, 100 mg, 120 mg, 140 mg, 150 mg, 160 mg, 180 mg, 190 mg, 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, 320 mg, 340 mg, 350 mg, 360 mg, 380 mg, 390 mg, 400 mg, 420 mg, 440 mg, 450 mg, 460 mg, 480 mg, 490 mg, 500 mg, or any two of the above values.

[0138] In one specific embodiment of the present invention, the polyunsaturated fatty acid includes docosahexaenoic acid (DHA) and eicosapentaenoic acid (ARA); the mass ratio of DHA to ARA is preferably 1:(0.8~1.5); optionally, the mass ratio of DHA to ARA is 1:0.8, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5 or any two of the above ratios.

[0139] In a specific embodiment of the present invention, the mass ratio of docosahexaenoic acid to eicosatraenoic acid is preferably 1:(0.9~1.2), more preferably 1:(0.9~1.1), and even more preferably 1:(0.95~1).

[0140] In one specific embodiment of the present invention, the infant formula milk powder may optionally include 1 g, 2 g, 3 g, 4 g, 4.2 g, 5 g, 6 g, 7 g, 8 g, 9 g, 10 g, 11 g, 12 g, 13 g, 14 g, 14.2 g, 15 g, 16 g, 17 g, 18 g, 19 g, 20 g, 21 g, 22 g, 23 g, 24 g of 1,3-dioleoyl-2-palmitoylglycerol, or any range between any two of the above values, per 100 g of infant formula milk powder.

[0141] In one specific embodiment of the present invention, per 100 g of infant formula milk powder, the infant formula milk powder includes 5-24 g of 1,3-dioleoyl-2-palmitoylglycerol, preferably 8-20 g of 1,3-dioleoyl-2-palmitoylglycerol, more preferably 10-20 g of 1,3-dioleoyl-2-palmitoylglycerol, even more preferably 10-15 g of 1,3-dioleoyl-2-palmitoylglycerol, even more preferably 13-14 g of 1,3-dioleoyl-2-palmitoylglycerol, and most preferably 13.2 g of 1,3-dioleoyl-2-palmitoylglycerol.

[0142] In one specific embodiment of the present invention, the infant formula milk powder may optionally include nucleotides of 1 mg, 5 mg, 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg, 50 mg, 55 mg, 60 mg, 65 mg, 80 mg, 90 mg, 100 mg or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0143] In one specific embodiment of the present invention, the infant formula milk powder may optionally include lactoferrin at a concentration of 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, 320 mg, 340 mg, 350 mg, 360 mg, 380 mg, 390 mg, 400 mg, 420 mg, 440 mg, 450 mg, 460 mg, 480 mg, 490 mg, 500 mg, 520 mg, 540 mg, 550 mg, 560 mg, 580 mg, 590 mg, 600 mg, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0144] In one specific embodiment of the present invention, the infant formula milk powder contains 470-540 mg of lactoferrin per 100 g of infant formula milk powder.

[0145] In one specific embodiment of the present invention, the infant formula milk powder further includes 200-500 mg of vitamins per 100 g of infant formula milk powder; optionally, the infant formula milk powder further includes vitamins of 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, 320 mg, 340 mg, 350 mg, 360 mg, 380 mg, 390 mg, 400 mg, 420 mg, 440 mg, 450 mg, 460 mg, 480 mg, 490 mg, 500 mg, or any range between two of the above values.

[0146] In a specific embodiment of the present invention, the vitamins, calculated per 100 g of infant formula, include: Vitamin A 300-400 μg RE; Vitamin D 10-15 μg; Vitamin E 1-15 mg α-TE; Vitamin K1 10-150 μg; Vitamin B1 300-800 μg; Vitamin B2 400-800 μg; Vitamin B6 200-600 μg; and Vitamin B2 200-600 μg. 12 0.5~10 μg; niacin and / or nicotinamide 2000~6000 μg; folic acid 30~100 μg; pantothenic acid 1500~3500 μg; vitamin C 10~100 mg; biotin 10~25 μg.

[0147] In one specific embodiment of the present invention, the vitamins may optionally include vitamin A at 300 μg RE, 310 μg RE, 320 μg RE, 330 μg RE, 340 μg RE, 350 μg RE, 360 μg RE, 370 μg RE, 380 μg RE, 385 μg RE, 390 μg RE, 400 μg RE, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0148] In one specific embodiment of the present invention, the vitamins may optionally include vitamin D at a concentration of 10 μg, 11 μg, 12 μg, 12.5 μg, 12.9 μg, 13 μg, 14 μg, 15 μg, or any two of the above values ​​per 100 g of infant formula.

[0149] In one specific embodiment of the present invention, the vitamins may optionally include vitamin E at a concentration of 1 mg α-TE, 2 mg α-TE, 3 mg α-TE, 4 mg α-TE, 5 mg α-TE, 6 mg α-TE, 7 mg α-TE, 8 mg α-TE, 9 mg α-TE, 10 mg α-TE, 11 mg α-TE, 12 mg α-TE, 13 mg α-TE, 14 mg α-TE, 15 mg α-TE, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0150] In one specific embodiment of the present invention, the vitamins may optionally include vitamin K1 at a concentration of 10 μg, 20 μg, 30 μg, 40 μg, 50 μg, 60 μg, 70 μg, 80 μg, 90 μg, 100 μg, 110 μg, 120 μg, 130 μg, 140 μg, 150 μg, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0151] In one specific embodiment of the present invention, the vitamins may optionally include vitamin B1 at a concentration of 300 μg, 350 μg, 400 μg, 450 μg, 500 μg, 550 μg, 600 μg, 650 μg, 700 μg, 750 μg, 800 μg, or any range between two of the above values, per 100 g of infant formula milk powder.

[0152] In one specific embodiment of the present invention, the vitamins may optionally include vitamin B2 at a concentration of 400 μg, 450 μg, 500 μg, 550 μg, 600 μg, 650 μg, 700 μg, 750 μg, 800 μg, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0153] In one specific embodiment of the present invention, the vitamins may optionally include vitamin B6 at a concentration of 200 μg, 250 μg, 300 μg, 350 μg, 400 μg, 420 μg, 450 μg, 500 μg, 550 μg, 600 μg, 650 μg, 700 μg, 750 μg, 800 μg, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0154] In one specific embodiment of the present invention, optionally, the vitamins include vitamin B, calculated per 100 g of infant formula milk powder. 120.5 μg, 1 μg, 1.5 μg, 2 μg, 2.5 μg, 2.6 μg, 3 μg, 3.5 μg, 4 μg, 4.5 μg, 5 μg, 5.5 μg, 6 μg, 6.5 μg, 7 μg, 7.5 μg, 8 μg, 8.5 μg, 9 μg, 9.5 μg, 10 μg, or any range between two of the above values.

[0155] In one specific embodiment of the present invention, the vitamins may optionally include 2000 μg, 2500 μg, 3000 μg, 3500 μg, 4000 μg, 4500 μg, 5000 μg, 5500 μg, 6000 μg of niacin and / or niacinamide, or any range between two of the above values, per 100 g of infant formula milk powder.

[0156] In one specific embodiment of the present invention, the vitamins may optionally include folic acid at a concentration of 30 μg, 35 μg, 40 μg, 45 μg, 50 μg, 55 μg, 60 μg, 65 μg, 70 μg, 75 μg, 79 μg, 80 μg, 85 μg, 90 μg, 95 μg, 100 μg, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0157] In one specific embodiment of the present invention, the vitamins may optionally include pantothenic acid at a concentration of 1500 μg, 1800 μg, 2000 μg, 2200 μg, 2500 μg, 2800 μg, 2850 μg, 3000 μg, 3200 μg, 3500 μg, or any two of the above values, per 100 g of infant formula milk powder.

[0158] In one specific embodiment of the present invention, the vitamins may optionally include vitamin C at a concentration of 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg, 50 mg, 55 mg, 60 mg, 65 mg, 70 mg, 75 mg, 80 mg, 85 mg, 90 mg, 95 mg, 100 mg, or any range between two of the above values, calculated per 100 g of infant formula milk powder.

[0159] In one specific embodiment of the present invention, the vitamins may optionally include biotin at a concentration of 10 μg, 11 μg, 12 μg, 13 μg, 14 μg, 15 μg, 16 μg, 17 μg, 18 μg, 19 μg, 20 μg, 21 μg, 22 μg, 23 μg, 24 μg, 25 μg, or any two of the above values, per 100 g of infant formula milk powder.

[0160] In one specific embodiment of the present invention, the infant formula milk powder further comprises 10-200 mg of vitamin-like substances per 100 g of infant formula milk powder; optionally, the infant formula milk powder further comprises 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg, 50 mg, 55 mg, 60 mg, 65 mg, 70 mg, 75 mg, 80 mg, 85 mg, 90 mg, 95 mg, 100 mg, 115 mg, 120 mg, 125 mg, 130 mg, 135 mg, 140 mg, 145 mg, 150 mg, 155 mg, 160 mg, 165 mg, 170 mg, 175 mg, 180 mg, 185 mg, 190 mg, 195 mg, 100 mg, 200 mg, or any two of the above values ​​per 100 g of infant formula milk powder.

[0161] In one specific embodiment of the present invention, the vitamin-like substance includes choline and taurine; the preferred mass ratio of choline to taurine is (1~5):1; optionally, the mass ratio of choline to taurine is 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, 4.5:1, 5:1 or any two of the above ratios.

[0162] In one specific embodiment of the present invention, preferably, the mass ratio of choline to taurine is (3~3.5):1.

[0163] In one specific embodiment of the present invention, the infant formula milk powder further includes 0.5 g, 0.6 g, 0.7 g, 0.8 g, 0.9 g, 1 g, 1.1 g, 1.2 g, 1.3 g, 1.4 g, 1.5 g, 1.6 g, 1.7 g, 1.8 g, 1.9 g, 2 g of functional oligosaccharides per 100 g of infant formula milk powder, or a range between any two of the above values.

[0164] In one specific embodiment of the present invention, the functional oligosaccharide includes galactooligosaccharide and fructooligosaccharide; the preferred mass ratio of galactooligosaccharide to fructooligosaccharide is (1~6):1; optionally, the mass ratio of galactooligosaccharide to fructooligosaccharide is 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, 4.5:1, 5:1, 5.5:1, 6:1 or any two of the above ratios.

[0165] In a specific embodiment of the present invention, preferably, the mass ratio of galactooligosaccharide to fructooligosaccharide is (3~4.5):1.

[0166] In one specific embodiment of the present invention, the infant formula milk powder comprises 10-23 g of protein, 15-29 g of fat, 2-5 g of linoleic acid, 200-600 mg of α-linolenic acid, and 50-58 g of carbohydrates per 100 g of infant formula milk powder.

[0167] In one specific embodiment of the present invention, the infant formula milk powder may optionally contain 10 g, 10.5 g, 11 g, 12 g, 13 g, 14 g, 15 g, 16 g, 17 g, 18 g, 19 g, 20 g, 21 g, 22 g, 23 g of protein per 100 g of infant formula milk powder, or a range between any two of the above values.

[0168] In one specific embodiment of the present invention, the protein sources in the infant formula milk powder include one or more of cow's milk, whole milk powder, skim milk powder, demineralized whey powder and whey protein powder, as well as α-lactalbumin powder and β-casein powder.

[0169] In one specific embodiment of the present invention, the infant formula milk powder may optionally contain 15 g, 16 g, 17 g, 18 g, 19 g, 20 g, 21 g, 22 g, 23 g, 24 g, 25 g, 26 g, 27 g, 27.9 g, 28 g, 29 g of fat or any two of the above values ​​per 100 g of infant formula milk powder.

[0170] In one specific embodiment of the present invention, the source of fat in the infant formula includes animal fat, vegetable oil and 1,3-dioleoyl-2-palmitoylglycerol; the animal fat includes milk fat and / or anhydrous butter; the vegetable oil includes one or more of sunflower seed oil, corn oil, soybean oil, coconut oil, low erucic acid rapeseed oil and flaxseed oil.

[0171] In one specific embodiment of the present invention, the infant formula milk powder may optionally contain 4 g, 4.1 g, 4.2 g, 4.3 g, 4.4 g, 4.5 g, 4.6 g, 4.7 g, 4.8 g, 4.9 g, 5 g of linoleic acid per 100 g of infant formula milk powder, or a range between any two of the above values.

[0172] In one specific embodiment of the present invention, the infant formula milk powder may optionally include 200 mg, 220 mg, 240 mg, 250 mg, 260 mg, 280 mg, 290 mg, 300 mg, 320 mg, 340 mg, 350 mg, 360 mg, 380 mg, 390 mg, 400 mg, 420 mg, 440 mg, 450 mg, 460 mg, 480 mg, 490 mg, 500 mg, 520 mg, 540 mg, 550 mg, 560 mg, 580 mg, 590 mg, 600 mg of α-linolenic acid or a range between any two of the above values, calculated per 100 g of infant formula milk powder.

[0173] In one specific embodiment of the present invention, the infant formula milk powder may optionally include 50 g, 51 g, 52 g, 53 g, 54 g, 55 g, 55.4 g, 56 g, 57 g, 58 g of carbohydrates or any two of the above values ​​per 100 g of infant formula milk powder.

[0174] In one specific embodiment of the present invention, the infant formula milk powder comprises 10-15 g of protein, 25-29 g of fat, 4-5 g of linoleic acid, 400-450 mg of α-linolenic acid, and 55-56 g of carbohydrates per 100 g of infant formula milk powder.

[0175] In one specific embodiment of the present invention, the infant formula milk powder contains 10.5 g of protein, 27.9 g of fat, 4.2 g of linoleic acid, 420 mg of α-linolenic acid, and 55.4 g of carbohydrates per 100 g of infant formula milk powder.

[0176] In one specific embodiment of the present invention, the raw materials of the infant formula milk powder, calculated per 100 g of infant formula milk powder, further include 1-35 g of lactose; optionally, the raw materials of the infant formula milk powder, calculated per 100 g of infant formula milk powder, further include 1 g, 2 g, 3 g, 4 g, 5 g, 6 g, 7 g, 8 g, 9 g, 10 g, 11 g, 12 g, 13 g, 14 g, 15 g, 16 g, 17 g, 18 g, 19 g, 20 g, 21 g, 22 g, 23 g, 24 g, 25 g, 26 g, 27 g, 28 g, 29 g, 30 g, 31 g, 32 g, 33 g, 34 g, 35 g of lactose or any two of the above values.

[0177] In one specific embodiment of the present invention, the raw materials of the infant formula milk powder, calculated per 100 g of infant formula milk powder, further include 0.01~0.25 g of probiotics; optionally, the raw materials of the infant formula milk powder, calculated per 100 g of infant formula milk powder, further include 0.01 g, 0.02 g, 0.03 g, 0.04 g, 0.05 g, 0.06 g, 0.07 g, 0.08 g, 0.09 g, 0.1 g, 0.11 g, 0.12 g, 0.13 g, 0.14 g, 0.15 g, 0.16 g, 0.17 g, 0.18 g, 0.19 g, 0.2 g, 0.21 g, 0.22 g, 0.23 g, 0.24 g, 0.25 g of probiotics, or any range between two of the above values.

[0178] The probiotics can be any probiotics known to those skilled in the art, and there are no special restrictions. In a specific embodiment provided by the present invention, preferably, the probiotics include Bifidobacterium.

[0179] In one specific embodiment of the present invention, the raw materials of the infant formula milk powder, calculated per 100 g of infant formula milk powder, further include 0.01~0.5 g of soybean lecithin; optionally, the raw materials of the infant formula milk powder may further include 0.01 g, 0.05 g, 0.1 g, 0.15 g, 0.2 g, 0.25 g, 0.3 g, 0.35 g, 0.4 g, 0.45 g, 0.5 g of soybean lecithin or any range between two of the above values.

[0180] In a specific embodiment of the present invention, the raw materials per 100 g of infant formula milk powder include: 10-200 g of raw milk; 1-35 g of lactose; 0-30 g of whole milk powder; 0-32.5 g of skim milk powder; 10-45 g of demineralized whey powder; 0.1-17 g of whey protein powder; 1-10 g of sunflower seed oil; 0.1-4 g of corn oil; 0.5-10 g of soybean oil; 0-3 g of coconut oil; 0-8.5 g of low-erucic acid rapeseed oil; 0-2 g of flaxseed oil; 1-24 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride; 0.5-5.5 g of α-whey protein powder; 0.5-4 g of β-casein powder; 0.1-0.5 g of soybean lecithin; 0-0.2 g of anhydrous butter; 0.1-2.5 g of fructooligosaccharide powder; and 0.1-10 g of galactooligosaccharide syrup. g; docosahexaenoic acid 0.1~2 g; eicosatraenoic acid 0.1~2.5 g; bifidobacteria 0~0.25 g; lactoferrin 0.2~0.6 g; nucleotides 0.01~0.1 g; compound nutrients containing vitamins, vitamin-like substances and minerals 0.7~2.5 g.

[0181] In a specific embodiment of the present invention, the raw materials per 100 g of infant formula milk powder include: 50-100 g of raw milk; 10-25 g of lactose; 0-30 g of whole milk powder; 0-32.5 g of skim milk powder; 30-45 g of demineralized whey powder; 0.1-1 g of whey protein powder; 5-10 g of sunflower seed oil; 1-2 g of corn oil; 5-10 g of soybean oil; 0-3 g of coconut oil; 0-8.5 g of low-erucic acid rapeseed oil; 0-2 g of flaxseed oil; 10-15 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride; 1.5-3.5 g of α-whey protein powder; 1-2 g of β-casein powder; 0.1-0.5 g of soybean lecithin; 0-0.2 g of anhydrous butter; 0.1-1 g of fructooligosaccharide powder; and 1-3 g of galactooligosaccharide syrup. g; docosahexaenoic acid 0.8~1.5 g; eicosatraenoic acid 1~1.5 g; bifidobacteria 0~0.25 g; lactoferrin 0.47~0.6 g; nucleotides 0.05~0.07 g; compound nutrients containing vitamins, vitamin-like substances and minerals 0.7~2.5 g.

[0182] In one specific embodiment of the present invention, the raw materials per 100 g of infant formula milk powder include: 80 g of raw milk; 23.9 g of lactose; 33 g of demineralized whey powder; 0.4 g of whey protein powder; 8.25 g of sunflower seed oil; 1.65 g of corn oil; 9.9 g of soybean oil; 13.2 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride; 2.8 g of α-whey protein powder; 1.2 g of β-casein powder; 0.3 g of soybean lecithin; 0.55 g of fructooligosaccharide powder; 2.3 g of galactooligosaccharide syrup; 1.06 g of docosahexaenoic acid; 1.27 g of eicosapentaenoic acid; 0.47-0.54 g of lactoferrin; 0.063 g of nucleotides; and 0.7-2.5 g of compound nutrients containing vitamins, vitamin-like substances, and minerals.

[0183] In a specific embodiment of the present invention, the compound nutrients, calculated per 100 g of infant formula milk powder, include: Vitamin A: 385 μg RE; Vitamin D: 12.9 μg; Vitamin E: 7 mg α-TE; Vitamin K1: 70 μg; Vitamin B1: 550 μg; Vitamin B2: 600 μg; Vitamin B6: 420 μg; Vitamin B... 12 2.6 μg; Niacin and / or Nicotinamide: 4000 μg; Folic Acid: 79 μg; Pantothenic Acid: 2850 μg; Vitamin C: 65 mg; Biotin: 16 μg; Choline: 130 mg; Taurine: 40 mg; Sodium: 190 mg; Potassium: 420 mg; Copper: 386 μg; Magnesium: 32 mg; Iron: 4.75 mg; Zinc: 3.77 mg; Manganese: 50 μg; Calcium: 350 mg; Phosphorus: 220 mg; Iodine: 98 μg; Chlorine: 325 mg; Selenium: 19.5 μg.

[0184] In this invention, the infant formula milk powder is suitable for infants and children aged 0-7 years.

[0185] In this invention, the infant formula milk powder can be prepared according to methods well known in the art, without any special limitations. Specifically, it can be prepared according to the following method: milk that has been coarsely filtered, homogenized and sterilized, powdered raw materials and melted oil raw materials are mixed, compound nutrients are added to obtain a mixed liquid; the mixed liquid is filtered, homogenized, cooled, concentrated and sterilized, spray dried and fluidized bed dried and cooled to obtain dried milk powder, which is then mixed with DHA, ARA, lactoferrin, nucleotides and bifidobacteria, and sieved to obtain the infant formula milk powder.

[0186] The increased richness and number of species in an infant's gut microbiota constitute a diverse and stable "microbial ecosystem," enhancing the intestinal barrier, promoting healthy development and balance of the immune system (reducing the risk of allergies and autoimmune diseases), and more efficiently digesting nutrients and synthesizing vitamins. Bifidobacterium breve, as a dominant core species in infancy, exhibits particularly prominent advantages in its high abundance. It excels at breaking down human milk oligosaccharides (HMOs), effectively inhibiting pathogens, preventing diarrhea and constipation, and significantly reducing the risk of allergies such as atopic dermatitis by regulating immune responses. It serves as the "cornerstone" for laying a healthy foundation for the entire beneficial microbiota. In a specific embodiment of this invention, the infant formula milk powder enhances intestinal comfort by promoting the growth of healthy gut microbiota; specifically, the healthy gut microbiota includes one or more of the following phyla: Bacteroidetes, Actinobacteria, Ruminococcus, Faecalibacterium, Bifidobacterium, and Lactobacillus; the Bifidobacterium genus includes Bifidobacterium breve.

[0187] In one specific embodiment of the present invention, the intestinal healthy flora includes Bifidobacterium; the Bifidobacterium includes Bifidobacterium breve.

[0188] To further illustrate the present invention, the following describes in detail, with reference to embodiments, an infant formula milk powder that improves intestinal comfort and flora distribution in infants and provides better feeding results.

[0189] All reagents used in the following examples are commercially available.

[0190] Example 1 and Comparative Example 1

[0191] Prepare formula milk powder using the following ingredients.

[0192] The ingredients of 100g of formula milk powder include:

[0193] Raw milk 80 g;

[0194] Lactose 23.9g;

[0195] 33 g of demineralized whey powder;

[0196] 0.4 g of whey protein powder;

[0197] Sunflower seed oil 8.25 g;

[0198] 1.65 g of corn oil;

[0199] 9.9 g of soybean oil;

[0200] 1,3-Dioleoyl-2-palmitoylglycerol triglyceride 13.2 g;

[0201] α-Whey protein powder 2.8 g;

[0202] 1.2 g of β-casein powder;

[0203] Soybean lecithin 0.3 g;

[0204] 0.55 g of fructooligosaccharide powder;

[0205] 2.3 g of galactooligosaccharide syrup;

[0206] Lactoferrin 0.54 g;

[0207] Nucleotides 0.063 g;

[0208] Docosahexaenoic acid (DHA) 1.06 g;

[0209] Eicosatraenoic acid (ARA) 1.27 g;

[0210] Vitamin A: 385 μg RE; Vitamin D: 12.9 μg; Vitamin E: 7 mg α-TE; Vitamin K1: 70 μg; Vitamin B1: 550 μg; Vitamin B2: 600 μg; Vitamin B6: 420 μg; Vitamin B1: 420 μg; 12 2.6 μg; Niacin and / or Nicotinamide: 4000 μg; Folic Acid: 79 μg; Pantothenic Acid: 2850 μg; Vitamin C: 65 mg; Biotin: 16 μg; Choline: 130 mg; Taurine: 40 mg; Sodium: 190 mg; Potassium: 420 mg; Copper: 386 μg; Magnesium: 32 mg; Iron: 4.75 mg; Zinc: 3.77 mg; Manganese: 50 μg; Calcium: 350 mg; Phosphorus: 220 mg; Iodine: 98 μg; Chlorine: 325 mg; Selenium: 19.5 μg.

[0211] From birth, infants were divided into an experimental group (Example 1) and a breast milk group based on the products they were fed. The specific trial included 177 infants aged 6 to 12 months (i.e., the time of data collection), with 60 infants in the breast milk group. The length and weight data of each group were collected at birth and at the time of questionnaire interview.

[0212] Z-scores, including WAZ, HAZ, WHZ, and zBMI, were calculated for each group of infants based on their height, weight growth, and age. Additionally, the difference between weight and birth weight, and the difference between height and birth length were calculated. Propensity score matching was then performed, and the results before and after matching are shown in Table 1. Figure 1 and Figure 2 As shown, where Figure 1 This is a distribution chart of infant growth and development indicators before propensity score matching. Figure 1 *: p<0.05; **: p<0.01; ***: p<0.001; NS: no significant difference; Figure 2 This is a distribution chart of infant growth and development indicators after propensity score matching. Figure 2 *: p<0.05; **: p<0.01; ***: p<0.001; NS: no significant difference).

[0213] Weight-for-Age Z-score (WAZ): By comparing an individual child's weight with the standard weight distribution of children of the same age and sex globally or regionally, the standard deviation (Z-score) from the mean is calculated, thereby quickly determining whether a child is underweight, normal, or overweight / obese.

[0214] Height-for-Age Z-score (HAZ): By comparing an individual child's height (or length) with the standard growth data of children of the same age and sex, the standard deviation (Z-score) of the deviation from the mean is calculated, thereby quickly identifying conditions such as growth retardation, normal growth, or excessive growth.

[0215] Weight-for-Height Z-score (WHZ): By comparing an individual child's weight with the standard weight distribution of children of the same height and sex, the standard deviation (Z-score) of the deviation from the mean is calculated. It can intuitively reflect whether the child's current nutritional status is balanced, and is especially suitable for quickly detecting short-term nutritional intake abnormalities.

[0216] Age-for-Age Z-score: This score determines whether a child's BMI is within the normal range by comparing the child's BMI to the standard BMI of children of the same age and sex, and calculating the standard deviation (Z-score) from the mean.

[0217] From Table 1 and Figure 1 Before matching, the analysis of differences in infant growth indicators between groups showed that the birth weight of infants in the experimental group was significantly lower than that in the breastfed group, while there was no significant difference in birth length between the two groups; there were no significant differences in age-specific weight Z-score (WAZ) and postnatal weight gain between the two groups; the age-specific length Z-score (HAZ) and postnatal length gain of infants in the experimental group were significantly higher than those in the breastfed group; the length-specific weight Z-score (WHZ) and age-specific BMI Z-score (zBMI) of the experimental group were significantly lower than those in the breastfed group; the analysis of the absolute values ​​of each Z-score after matching showed (Table 1, Figure 1 There was no significant difference in the absolute value of WAZ between the two groups; however, the absolute value of HAZ in the experimental group was significantly higher than that in the breast milk group. In Table 1, M represents the mean and S represents the standard deviation.

[0218] Table 1. Comparison of growth and development indicators of infants with different feeding methods before and after propensity score matching (PSM).

[0219]

[0220] A. Pairwise comparisons of data before matching showed statistically significant differences between the experimental group and the breast milk group (p<0.05).

[0221] a. After matching, pairwise comparisons of the data showed statistically significant differences between the experimental group and the breast milk group (p<0.05).

[0222] From Table 1, Figure 1 and Figure 2 It can be seen that the experimental group and the breastfed group had similar growth processes, and the weight Z-score and growth curve were more consistent with those of breastfed babies, which were lower than those of the control group, significantly reducing the risk of excessive weight gain in early life. The experimental group had an average increase of 1.49 Z-scores in height, which significantly promoted infant growth and was much higher than that of the control group.

[0223] Comparison of gut microbiota indicators among different groups of infants

[0224] Stool samples were collected from 30 infants in each group, ranging in age from 6 to 12 months, for intestinal flora analysis (using 16S rRNA). Results were obtained. Figures 3-8 As shown. Among them. Figure 3 A graph showing the results of phylum-level microbial community testing in infant fecal samples; Figure 4 Heatmap of species abundance at the phylum level in infant fecal samples. Figure 5 This is a graph showing the results of the detection of horizontal microbiota in an infant's stool sample. Figure 6 Heatmap of species abundance of genus-level microbial communities in infant fecal samples. Figure 7 A graph showing the results of the detection of microbial community at different levels in infant fecal samples; Figure 8 A heatmap showing the species abundance of horizontal microbial communities in infant fecal samples.

[0225] Depend on Figures 3-8The fecal samples from the experimental group showed higher abundances of Firmicutes (28.68%), Bifidobacterium (61.97%), and Bifidobacterium breve (22.28%). The species abundance heatmap revealed that the gut microbiota of the experimental group infants was mainly enriched in: Bacteroides, Actinobacteria, Ruminococcus, Faecalibacterium, Bifidobacterium, Bifidobacterium breve, and Lactobacillus mucosae.

[0226] Infant feeding difficulties

[0227] The infants' feeding status was assessed using a feeding difficulty scale, and propensity score matching was performed to obtain box plots of feeding difficulty scores for infants in different feeding method groups before and after matching, as shown in the figure. Figure 9 As shown, (a) is before matching, and (b) is after matching. Figure 9 *: p<0.05; **: p<0.01; ***: p<0.001; NS: no significant difference. Figure 9 It can be seen that the scores of infants in the experimental group were lower than those in the breastfed group before and after matching, indicating that the infants in the experimental group had less feeding difficulties and were easier to feed.

[0228] Infant bowel movements and gas

[0229] Analysis of differences in bowel movements among infants aged 6–12 months between groups showed (Table 2, ...). Figure 10 To match the previous infant bowel movement distribution bar chart. Figure 11 To create a bar chart showing the distribution of infant bowel movements after matching, Figure 10 and Figure 11 *: p<0.05; **: p<0.01; ***: p<0.001; NS.: no significant difference). Before and after matching, there was a significant difference in the frequency of bowel movements between the two groups, with the proportion of infants having one bowel movement per day in the experimental group (89%) being higher than that in the breastfed group (65%). Before and after matching, there was no significant difference in the proportion of infants experiencing gas in the past week between the experimental and breastfed groups. Regarding the incidence of green stools in the past week, there was no significant difference between the experimental and breastfed groups after matching.

[0230] Table 2 Comparison of bowel movements among infants of different feeding methods before and after propensity score matching (PSM).

[0231]

[0232] A. Pairwise comparisons of pre-matching data showed statistically significant differences between the experimental and breast milk groups (p<0.05).

[0233] a. After matching, pairwise comparisons of the data showed statistically significant differences between the experimental group and the breast milk group (p<0.05).

[0234] In summary, the infant formula provided by this invention can promote infant growth and development, improve intestinal comfort, reduce feeding difficulty index scores, promote the abundance of Bifidobacterium and Bifidobacterium breve in the infant gut, and significantly increase the richness and species number of the infant gut microbiota, creating a microbiota state that is closer to that of breastfed infants.

[0235] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An infant formula milk powder, characterized in that, Based on 100g of infant formula, it includes: 500-2000mg of minerals, 20-500mg of polyunsaturated fatty acids, 1-24g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100mg of nucleotides and 200-600mg of lactoferrin. The minerals include 2-6 mg of iron, 1-5 mg of zinc, and 300-500 mg of calcium.

2. The infant formula milk powder according to claim 1, characterized in that, Based on 100g of infant formula, it includes: 500-2000 mg of minerals, 20-500 mg of polyunsaturated fatty acids, 1-24 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100 mg of nucleotides, and 360-600 mg of lactoferrin. The minerals include 4-6 mg of iron, 3-4 mg of zinc, and 300-400 mg of calcium; Preferably, per 100 g of infant formula milk powder, it includes: 500-2000 mg of minerals, 20-500 mg of polyunsaturated fatty acids, 1-10 g of 1,3-dioleoyl-2-palmitoylglycerol triglyceride, 1-100 mg of nucleotides and 470-540 mg of lactoferrin. The minerals include 4.75 mg of iron, 3.77 mg of zinc, and 350 mg of calcium.

3. The infant formula milk powder according to claim 1 or 2, characterized in that, The infant formula milk powder also includes 200-500 mg of vitamins per 100 g of formula milk powder; And / or, per 100 g of infant formula, the infant formula also includes 10-200 mg of vitamin-like substances; And / or, per 100 g of infant formula, the infant formula may further include 0.5-2 g of functional oligosaccharides.

4. The infant formula milk powder according to claim 3, characterized in that, The polyunsaturated fatty acids include docosahexaenoic acid (DHA) and eicosatraenoic acid (EAA); the mass ratio of DHA to EEA is 1:(0.8~1.5); preferably, the mass ratio of DHA to EEA is 1:(0.9~1.1). The vitamin-like substance includes choline and taurine; the mass ratio of choline to taurine is (1~5):1; preferably, the mass ratio of choline to taurine is (3~3.5):

1. The functional oligosaccharides include galactooligosaccharides and fructooligosaccharides; the mass ratio of galactooligosaccharides to fructooligosaccharides is (1~6):1, preferably, the mass ratio of galactooligosaccharides to fructooligosaccharides is (3~4.5):

1.

5. The infant formula milk powder according to claim 3, characterized in that, The vitamins, calculated per 100 g of formula milk powder, include: Vitamin A 300~400 μg RE; Vitamin D 10~15 μg; Vitamin E 1~15 mg α-TE; Vitamin K1 10~150 μg; Vitamin B1 300~800 μg; Vitamin B2 400~800 μg; Vitamin B6 200~600 μg Vitamin B 12 0.5~10 μg; Nicotinic acid and / or nicotinamide 2000~6000 μg; Folic acid 30~100 μg; Pantothenic acid 1500~3500 μg; Vitamin C 10~100 mg; Biotin 10~25 μg; And / or, the minerals include: Sodium 100~300 mg; Potassium 200-600 mg; Copper 100~400 μg; Magnesium 10~50 mg; Iron 4~6 mg; Zinc 3~4 mg; Manganese 5~60 μg; Calcium 300-400 mg; Phosphorus 100~300 mg; Iodine 1~100 μg; Chlorine 100~400 mg; Selenium 10~30 μg.

6. The infant formula milk powder according to claim 5, characterized in that, Based on 100 g of infant formula milk powder, the infant formula milk powder includes 10-23 g of protein, 15-29 g of fat, 2-5 g of linoleic acid, 200-600 mg of α-linolenic acid, and 50-58 g of carbohydrates.

7. The infant formula milk powder according to claim 6, characterized in that, Based on 100 g of infant formula milk powder, the infant formula milk powder includes 10-15 g of protein, 25-29 g of fat, 4-5 g of linoleic acid, 400-450 mg of α-linolenic acid, and 55-56 g of carbohydrates.

8. The infant formula milk powder according to claim 6, characterized in that, The protein sources in the infant formula milk powder include one or more of cow's milk, whole milk powder, skim milk powder, demineralized whey powder and whey protein powder, as well as α-lactalbumin powder and β-casein powder; The fat sources in the infant formula include animal fats, vegetable oils, and 1,3-dioleoyl-2-palmitoylglycerol triglycerides; the animal fats include milk fat and / or anhydrous butter; the vegetable oils include one or more of sunflower oil, corn oil, soybean oil, coconut oil, low-erucic acid rapeseed oil, and flaxseed oil. And / or, per 100 g of infant formula, the raw materials of the infant formula also include 1-35 g of lactose; And / or, per 100 g of infant formula, the raw materials of the infant formula also include 0.01~0.25 g of probiotics; preferably, the probiotics include Bifidobacterium.

9. The infant formula milk powder according to any one of claims 1 to 8, characterized in that, The ingredients of infant formula milk powder per 100g include: Raw milk 10~200 g; Lactose 1~35 g; Whole milk powder 0~30 g; Skim milk powder 0~32.5 g; 10-45 g of demineralized whey powder; Whey protein powder 0.1~17 g; Sunflower seed oil 1~10 g; Corn oil 0.1~4 g; Soybean oil 0.5~10 g; Coconut oil 0~3 g; Low erucic acid rapeseed oil 0~8.5 g; Flaxseed oil 0~2 g; 1,3-Dioleoyl-2-palmitoylglycerol triglyceride 1~24 g; α-Whey protein powder 0.5~5.5 g; β-casein powder 0.5~4 g; Soybean lecithin 0.1~0.5 g; Anhydrous butter 0~0.2 g; 0.1~2.5 g of fructooligosaccharide powder; 0.1-10 g of galactooligosaccharide syrup; 0.1~2 g of docosahexaenoic acid; Eicosatraenoic acid 0.1~2.5 g; Bifidobacteria 0~0.25 g; Lactoferrin 0.2~0.6 g; Nucleotides 0.01~0.1 g; 0.7~2.5 g of a complex of nutrients containing vitamins, vitamin-like substances and minerals; Preferably, the compound nutrients include: Vitamin A: 385 μg RE; Vitamin D: 12.9 μg; Vitamin E: 7 mg α-TE; Vitamin K1: 70 μg; Vitamin B1: 550 μg; Vitamin B2: 600 μg; Vitamin B6: 420 μg; Vitamin B 12 2.6 μg; Nicotinic acid and / or nicotinamide: 4000 μg; Folic acid: 79 μg; Pantothenic acid: 2850 μg; Vitamin C: 65 mg; Biotin: 16 μg; Choline: 130 mg; Taurine: 40 mg; Sodium: 190 mg; Potassium: 420 mg; Copper: 386 μg; Magnesium: 32 mg; Iron: 4.75 mg; Zinc: 3.77 mg; Manganese: 50 μg; Calcium: 350 mg; Phosphorus: 220 mg Iodine: 98 μg; Chlorine: 325 mg Selenium: 19.5 μg.

10. The infant formula milk powder according to claim 9, characterized in that, The infant formula milk powder improves intestinal comfort by promoting the growth of healthy gut flora.

Images