Sports nutritional food with turbidity removing and stone dissolving functions

Abstract

The invention relates to the technical field of food science, and particularly discloses a sports nutritious food with functions of removing turbidity and dissolving stones. The composition comprises medicinal and edible raw materials such as ginseng, astragalus membranaceus and saussurea involucrata cultures, and nutritional ingredients such as soybean peptide, branched chain amino acid, fructo-oligosaccharide and microencapsulated vitamins. The preparation method comprises the following steps: performing water extraction to obtain plant active ingredients, performing enzymolysis treatment and fermentation reinforcement to improve the bioavailability of the ingredients, and finally performing mixing, homogenizing and forming processes to obtain the product. The raw materials such as corn stigma, dandelion, endothelium corneum gigeriae galli and the like are matched for use, so that discharge of metabolic wastes of organisms can be promoted, and the normal physiological metabolism function can be maintained. According to the product disclosed by the invention, through the synergistic interaction effect of various active ingredients, comprehensive nutrition support can be provided for sports organisms; the formula has the advantages of scientific formula and comprehensive functions. In addition, according to the preparation method disclosed by the invention, by optimizing extraction, enzymolysis and fermentation processes, the dissolution rate and conversion efficiency of active ingredients are improved.

Description

Technical Field

[0001] This application relates to the field of food science and technology, and more specifically, to a sports nutrition food with the function of clearing turbidity and dissolving stones. Background Technology

[0002] Sports nutrition foods are specialized foods designed for athletes who experience high energy expenditure, active muscle metabolism, and high-intensity physiological functions. Their core function is to provide targeted nutritional support for energy replenishment during exercise, muscle repair, and fatigue recovery after exercise, helping to maintain athletic performance and reduce the risk of sports injuries. As athletes' nutritional needs become more refined, they require not only nutrients for rapid energy replenishment and muscle repair but also support for metabolic regulation, immune protection, and other physiological needs to adapt to the physical load brought about by different intensities of exercise. Simultaneously, high-intensity exercise leads to accelerated excretion of metabolic waste and increased urine concentration, making athletes prone to the accumulation of toxins in the body and even increasing the risk of urinary tract stones. However, existing sports nutrition foods generally lack functions related to clearing toxins and dissolving stones, failing to meet this specific physiological need.

[0003] Most existing sports nutrition products focus on supplementing single types of nutrients, such as simply adding branched-chain amino acids, carbohydrates, or vitamins. They lack a scientific combination of active ingredients from medicinal and edible plants with modern sports nutrients, making it difficult to achieve a synergistic effect of nutritional supply and physiological regulation. This singular formula design cannot comprehensively cover the body's multi-dimensional needs for energy replenishment, muscle repair, and metabolic regulation during exercise, resulting in limitations in meeting the dual needs of athletes for nutritional support, functional regulation, and litholysis under specific physiological conditions. Summary of the Invention

[0004] To address the problem that existing sports nutrition foods lack the synergistic ratio of active ingredients from medicinal and edible plants with modern sports nutrients, thus failing to fully meet the comprehensive needs of athletes for nutritional support, functional regulation, and litholysis under special physiological conditions, this application provides a sports nutrition food with litholysis function.

[0005] In the first aspect, this application provides a sports nutrition food with the function of clearing turbidity and dissolving stones, using the following technical solution:

[0006] A sports nutrition food with the function of clearing turbidity and dissolving stones contains the following ingredients by weight: ginseng 3-5 parts, astragalus 8-10 parts, snow lotus culture 3-5 parts, soybean peptide 6-8 parts, longan 4-6 parts, red dates 7-9 parts, dandelion 2-4 parts, houttuynia cordata 2-4 parts, corn silk 2-3 parts, chicken gizzard lining 2-3 parts, ginger powder 1-3 parts, honey 5-7 parts, apple juice 11-15 parts, polygonatum 4-6 parts, citric acid 1-2 parts, walnut oil 1-2 parts, potassium sorbate 0.1-0.3 parts, branched-chain amino acids 3-5 parts, fructooligosaccharides 6-8 parts, vitamin B1 0.5-1 part and vitamin B2 0.2-0.5 parts.

[0007] By employing the above technical solutions, ginseng, astragalus, snow lotus culture, and polygonatum provide plant active ingredients such as saponins and polysaccharides to regulate the body's metabolism; longan and red dates supplement mild nutrition; dandelion and houttuynia cordata clear heat, promote diuresis, detoxify, and relieve strangury; corn silk promotes diuresis, reduces swelling, and clears strangury; chicken gizzard lining resolves food stagnation, promotes digestion, and dissolves stones. These four ingredients work synergistically to clear turbidity, dissolve stones, and promote the excretion of metabolic waste and small stones. Branched-chain amino acids supplement key amino acids needed for muscle metabolism, and soybean peptides provide easily absorbed small molecule peptide nutrition. Fructooligosaccharides regulate the intestinal flora environment, and vitamins B1 and B2 supply coenzymes needed for energy metabolism. Honey and apple juice provide energy and natural flavor, walnut oil supplements unsaturated fatty acids, citric acid adjusts the product's taste, ginger powder harmonizes the characteristics of the ingredients, and potassium sorbate ensures the product's storage stability. All these specific substances work together in proportion to achieve the dual effects of providing multi-dimensional nutritional support and clearing turbidity and dissolving stones for athletes.

[0008] Preferably, the branched-chain amino acids include leucine, isoleucine and valine, in a weight ratio of (2.0-2.5):(0.8-1.2):(0.8-1.2); the vitamin B1 and vitamin B2 are present in microencapsulated form, with sodium alginate as the microcapsule wall material and an encapsulation rate of not less than 85%.

[0009] By adopting the above technical solution, the branched-chain amino acids are set in a specific ratio of leucine, isoleucine, and valine, with leucine having a relatively prominent proportion. This ratio, combined with the other two amino acids, is adapted to the needs of muscle metabolism, thus precisely supplying the key amino acids required for muscle synthesis and repair. Furthermore, sodium alginate is used as the wall material to microencapsulate vitamins B1 and B2, ensuring an encapsulation rate of no less than 85%. This protects against high temperatures, acidic or alkaline environments, and the erosion of gastrointestinal digestive fluids during processing. The combination of this specific ratio design and microencapsulation technology ensures the stability and effectiveness of the relevant nutrients in the product, while not affecting the synergistic effect of the clearing and dissolving components.

[0010] Preferably, the ginseng is artificially cultivated 4-5 year old ginseng, and the total content of ginsenosides Rg1 and Rb1 in the ginseng is not less than 1.5%; the snow lotus culture is calculated on a dry basis, and the polysaccharide content in the snow lotus culture is not less than 8%; the astragaloside A content in the astragalus is not less than 0.8%.

[0011] By adopting the above technical solutions, and by limiting ginseng to artificially cultivated 4-5 year old plants with a total content of ginsenosides Rg1 and Rb1 of not less than 1.5%, sufficient accumulation and stable supply of core active saponin components in ginseng are guaranteed; by limiting snow lotus culture to a polysaccharide content of not less than 8% on a dry basis, the effective concentration of its core active polysaccharide components and standardization of raw materials are guaranteed; by limiting the astragaloside A content in astragalus to not less than 0.8%, the supply of key active components in astragalus is guaranteed. The above-mentioned clear limitations on the growth cycle, morphology and core active component content of core medicinal and edible raw materials play a role in stabilizing raw material quality and ensuring an effective supply of active components, thereby achieving the effect of ensuring that the plant active components and the clearing and dissolving components in the product have the same basic function.

[0012] Preferably, the sports nutrition food is in the form of liquid, powder, or pills. When the product is in liquid form, the energy value per 100mL is 1000-1500kJ, the carbohydrate content is 10-20g, and the branched-chain amino acid content is 1-3g. When the product is in the form of powder or pills, the energy value per 100g is 1200-1600kJ, the carbohydrate content is 65-75g, and the branched-chain amino acid content is 2-4g.

[0013] By adopting the above technical solutions and setting the product in three forms—liquid, powder, or pill—it can meet the consumption needs of athletes in different usage scenarios. By limiting the energy value, carbohydrate, and branched-chain amino acid content ranges for different forms, the liquid form is suitable for immediate replenishment needs, while the powder and pill forms are suitable for portable storage and high-nutrient-density replenishment needs. The matching and setting of the above form design and the corresponding core nutrient content range can accurately adapt to different usage scenarios and ensure the targeted supply of core nutrients, thereby achieving the effect that different forms of products can meet the nutritional supplementation needs of athletes.

[0014] Secondly, this application provides a method for preparing a sports nutrition food with the function of clearing turbidity and dissolving stones, using the following technical solution:

[0015] A method for preparing a sports nutrition food with the function of clearing turbidity and dissolving stones includes the following steps:

[0016] S1. Raw material pretreatment: Ginseng, Astragalus, snow lotus culture, longan, red dates, dandelion, houttuynia cordata, corn silk, chicken gizzard lining and Polygonatum sibiricum are crushed into particles with a diameter of 0.1-1.0 mm and set aside.

[0017] S2, Water extraction: Mix the pretreated raw materials in S1, add purified water, and the mass ratio of purified water to mixed raw materials is 8:1 to 12:1. Extract at a temperature of 80-95℃ for 1-3 hours to obtain the extract.

[0018] S3. Enzymatic hydrolysis: Add a complex enzyme, including cellulase and protease, to the extract obtained in S2. The amount added is 0.1-0.5% of the weight of the extract. Hydrolyze for 1-2 hours at pH 5.0-6.5 and temperature 45-55℃. Then raise the temperature to 85℃ and hold for 10 minutes to inactivate the enzyme, and obtain the hydrolysate.

[0019] S4. Fermentation enhancement: Inoculate the enzymatic hydrolysate obtained in S3 with probiotics Lactobacillus, with an inoculation amount of 1-3% of the weight of the enzymatic hydrolysate. Ferment at 35-40℃ for 12-24 hours. After fermentation, heat to 70℃ and maintain for 5 minutes to terminate fermentation and obtain fermentation broth.

[0020] S5. Filtration and Concentration: Filter the fermentation broth obtained in S4 through a 100-200 mesh sieve to remove residue and obtain filtrate; concentrate the filtrate under a vacuum of 0.06-0.09 MPa and a temperature of 50-70℃ until the solid content is 40-60% to obtain concentrated liquid.

[0021] S6. Mixing and homogenization: Add the soybean peptides, ginger powder, honey, apple juice, citric acid, walnut oil, potassium sorbate, branched-chain amino acids, fructooligosaccharides, and microencapsulated vitamin B1 and vitamin B2 to the concentrate obtained in S5. Mix at a stirring speed of 200-500 r / min for 10-30 min, and then homogenize 1-3 times using a homogenizer at a pressure of 20-40 MPa to obtain a homogenized mixture.

[0022] S7. Molding and Packaging: The homogeneous mixture obtained in S6 is processed according to the product form.

[0023] For liquid forms, fill directly and seal;

[0024] For powder form, spray drying is performed with an inlet air temperature of 150-180℃ and an outlet air temperature of 70-90℃ to obtain powder, which is then compressed into tablets or filled into capsules.

[0025] For pill form, excipients are added, granulation is performed, and the mixture is dried at a temperature of 40-60℃ until the moisture content is less than 5%, and then formed into pills.

[0026] By employing the above technical solution, the contact area between the raw materials and the extraction medium is increased by pulverizing the medicinal and edible raw materials to a specific particle size, which particularly promotes the dissolution of active ingredients related to clearing turbidity and dissolving stone in dandelion, houttuynia cordata, corn silk, and chicken gizzard lining. Water extraction is then performed with a specific liquid-to-material ratio, temperature, and time to fully dissolve the active ingredients and clearing / dissolving stone components in the raw materials. By adding a compound enzyme and enzymatically hydrolyzing it under specific pH and temperature conditions, macromolecular substances in the raw materials are degraded. Subsequent temperature increases inactivate the enzyme to avoid over-hydrolysis. Then, probiotics such as lactobacillus are inoculated and fermented under suitable conditions to transform nutrients and increase beneficial metabolites. The process is terminated after fermentation to ensure product safety. The process involves filtering the food through a sieve with a specific mesh size to remove residues, concentrating it to a specified solid content under a specific vacuum and temperature to increase the concentration of nutrients, mixing various nutrients by setting specific stirring speeds and times, and then homogenizing it with specific pressures and cycles to ensure uniform dispersion of the components. Finally, appropriate molding and packaging methods are adopted according to different product forms. The above series of coherent and parameter-defined preparation steps work together to fully release the active ingredients of the raw materials, optimize the form of nutrients, and ensure the uniformity and stability of the product. This results in providing sports nutrition foods with controllable quality, easily utilized nutrients, and the ability to clear turbidity and dissolve stones for athletes.

[0027] Preferably, in step S2, the extraction process is carried out in two stages. The first extraction temperature is 85-90℃ and the time is 1-2 hours. The second extraction temperature is 90-95℃ and the time is 0.5-1.5 hours. The two extracts are combined. In the second extraction, 0.1-0.3% of the weight of the extract disodium ethylenediaminetetraacetate is added as a metal ion chelating agent.

[0028] By employing the above-mentioned technical solution and a two-stage extraction process, the first extraction uses a relatively mild temperature and a longer extraction time to allow the raw material tissue to fully swell and initially release heat-sensitive and water-soluble active ingredients. The second extraction involves appropriately increasing the temperature and using a shorter extraction time to further disrupt the plant cell structure and promote the dissolution of deeper components, thereby improving the overall extraction efficiency of the target components. A specific proportion of disodium ethylenediaminetetraacetate is added during the second extraction. Its chelating effect forms stable complexes with any metal ions present in the extract, inhibiting the catalytic oxidation reaction of metal ions and preventing undesirable binding with active ingredients, thus ensuring the stability of the extract system and the integrity of the effective components.

[0029] Preferably, in step S3, the mass ratio of cellulase to protease in the complex enzyme is 1:1 to 2:1, and the enzyme is pretreated with ultrasound before enzymatic hydrolysis. The ultrasound frequency is 20-40 kHz, and the treatment time is 5-15 min.

[0030] By employing the above technical solution and a composite enzyme system with a specific mass ratio, cellulase is used to specifically degrade the cellulose structure in plant cell walls, while protease breaks down protein components in the tissue. Together, these processes disrupt the physical barriers of the raw material cells and reduce extraction mass transfer resistance. Prior to enzymatic hydrolysis, ultrasonic pretreatment at a specific frequency and duration is introduced. The cavitation effect and mechanical vibration generated by ultrasound in the liquid medium initially disrupt the surface structure of the raw material particles, increasing their specific surface area and promoting sufficient contact between the enzyme and substrate. This creates favorable conditions for subsequent enzymatic hydrolysis, further improving the dissolution efficiency of the turbidity-dissolving components and active ingredients.

[0031] Preferably, in step S4, the probiotic lactobacillus is Lactobacillus plantarum, with a viable count of not less than 1×10⁻⁶. 9 The concentration is CFU / g, and fructooligosaccharides accounting for 0.5-1.5% of the fermentation liquid weight are added as prebiotics during the fermentation process.

[0032] By employing the above-mentioned technical solution, and utilizing its excellent acid resistance and enzyme-producing characteristics, it achieves efficient substrate transformation and enriches the composition of metabolites. Adding a specific proportion of fructooligosaccharides as a prebiotic during fermentation provides a preferential carbon source, promoting cell proliferation and maintaining the biological activity of the fermentation system. This technical approach, through the combination of strain selection and nutritional regulation, provides technical support for the stable progress of the fermentation process and the assurance of product quality.

[0033] Preferably, in step S6, the microencapsulated vitamin B1 and vitamin B2 are prepared by the following method: sodium alginate is prepared into a 3-5% solution, vitamin B1 and vitamin B2 are added, and microcapsules are prepared by spray drying at an inlet temperature of 160-180℃ and an outlet temperature of 70-85℃ to obtain microencapsulated vitamin B1 and vitamin B2.

[0034] By employing the above-mentioned technical solution, sodium alginate is prepared into a solution of a specific concentration and used as the wall material matrix. Its excellent film-forming properties and biocompatibility enable the formation of stable microcapsule structures. Spray drying technology is used for microencapsulation preparation. By controlling the inlet and outlet temperatures within a specific range, a dense film structure is rapidly formed on the droplet surface while preventing the core material from decomposing due to overheating, effectively encapsulating vitamins B1 and B2. This technical approach, through the selection of wall materials and the coordination of process parameters, provides technical assurance for the stability of vitamin components during subsequent processing and storage.

[0035] Preferably, in step S7, for powder form, maltodextrin is added as a carrier before spray drying, and the mass ratio of maltodextrin to homogenized mixture is 1:5 to 1:10; for pellet form, the added excipients include microcrystalline cellulose and magnesium stearate, the amount of microcrystalline cellulose added is 2-5% of the total mass of pellets, the amount of magnesium stearate added is 0.5-1.5% of the total mass of pellets, and after pellet formation, fluidized bed coating technology is used for coating, the coating material is hydroxypropyl methylcellulose, and the coating weight gain is 2-5%.

[0036] By employing the above technical solutions, and adding a specific proportion of maltodextrin as a carrier, its excellent film-forming properties and encapsulation ability are utilized to increase the glass transition temperature of the material and prevent it from sticking to the wall and clumping. In the preparation of pills, microcrystalline cellulose is added as a filler and binder, and magnesium stearate as a lubricant. Controlling the addition ratio of these two components improves material flowability and ensures the quality of pill formation. Fluidized bed coating technology is used with hydroxypropyl methylcellulose as the coating material. By controlling the coating weight gain ratio, a continuous and dense protective film is formed, improving product stability.

[0037] In summary, this application has the following beneficial effects:

[0038] 1. Because this application uses a specific combination of various medicinal and edible raw materials such as ginseng, astragalus, and snow lotus culture, along with branched-chain amino acids, fructooligosaccharides, and microencapsulated vitamins, and focuses on the addition of ingredients with the effects of clearing turbidity and dissolving stones, such as dandelion, houttuynia cordata, corn silk, and chicken gizzard lining, this combination forms a synergistic effect between plant active ingredients and modern sports nutrients, and achieves the targeted combination of clearing turbidity and dissolving stones ingredients, thus obtaining a technical effect that can more comprehensively meet the body's special nutritional, physiological regulation, and clearing turbidity and dissolving stones comprehensive needs during exercise.

[0039] 2. This application preferably employs vitamin microencapsulation technology, synergistic enzymatic hydrolysis with compound enzymes, and probiotic fermentation fortification process. Because microencapsulation effectively protects the activity of vitamins, and the combined use of enzymatic hydrolysis and fermentation significantly promotes the release and transformation of active ingredients in plant raw materials, the technical effect of simultaneously improving the stability of key components, bioavailability, and the efficacy of clearing turbidity and dissolving stones is achieved.

[0040] 3. The method of this application, through stepwise extraction, fermentation enhancement, precise homogenization and molding technology for different dosage forms, ensures the efficient extraction of active ingredients and the stability of the final product system, while maximizing the retention of the activity and synergistic effect of the clearing and dissolving components. Therefore, it achieves the technical effect of uniform and stable product quality, flexible adaptation to different consumer preferences and application scenarios, and clearing and dissolving functions. Attached Figure Description

[0041] Figure 1This is a flowchart of a preparation method for a sports nutrition food with the function of clearing turbidity and dissolving stones, provided in this application. Detailed Implementation

[0042] The present application will be further described in detail below with reference to embodiments and comparative examples. Unless otherwise specified, the experimental methods used below are conventional methods. Unless otherwise specified, the materials, reagents, methods and instruments used are all conventional materials, reagents, methods and instruments in the art, which can be obtained by those skilled in the art through commercial channels or prepared according to literature methods.

[0043] Technical concept:

[0044] Current sports nutrition products often suffer from limitations such as single-function limitations and insufficient synergy. This stems from the fact that formulation design frequently focuses solely on supplementing a single nutrient or on simple combinations based on traditional experience. It fails to adequately consider the complex physiological changes across multiple systems and targets during exercise, neglecting the risks of sediment buildup and kidney stones caused by the high metabolism and concentrated urine of active individuals. This singular approach makes it difficult for products to simultaneously achieve multiple goals, including energy supply, metabolic regulation, and functional recovery, thus failing to meet the comprehensive nutritional support requirements of modern sports science.

[0045] This technical solution constructs a scientifically compatible system of extracts from medicinal and edible plants and modern sports nutrients. Combined with stepwise extraction, enzymatic fermentation, and nutrient stabilization technology, it forms a multi-component synergistic effect encompassing nutritional supplementation, metabolic regulation, and litholytic / purification. Specifically, by optimizing the release efficiency and bioavailability of litholytic / purification components and nutrients, and establishing a complementary mechanism among plant active ingredients, essential nutrients, and litholytic / purification components, it achieves a technological transformation in sports nutrition support from simple supplementation to a systemic regulation of nutrition and health protection.

[0046] Example 1

[0047] This application provides a sports nutrition food with the function of clearing turbidity and dissolving stones, which contains the following ingredients by weight:

[0048] Ginseng 4 parts, Astragalus 9 parts, Snow Lotus culture 4 parts, Soybean peptide 7 parts, Longan 5 parts, Red dates 8 parts, Dandelion 3 parts, Houttuynia cordata 3 parts, Corn silk 2.5 parts, Chicken gizzard lining 2.5 parts, Ginger powder 2 parts, Honey 6 parts, Apple juice 13 parts, Polygonatum 5 parts, Citric acid 1.5 parts, Walnut oil 1.5 parts, Potassium sorbate 0.2 parts, Branched-chain amino acids 4 parts, Fructooligosaccharides 7 parts, Vitamin B1 0.75 parts and Vitamin B2 0.35 parts.

[0049] Among them, the branched-chain amino acids include leucine, isoleucine and valine, with a weight ratio of 2.25:1:1; vitamin B1 and vitamin B2 exist in microencapsulated form, with sodium alginate as the microcapsule wall material and an encapsulation rate of not less than 85%.

[0050] Among them, the ginseng is artificially cultivated 4-5 year old ginseng, and the total content of ginsenosides Rg1 and Rb1 in the ginseng is 1.5%; the snow lotus culture is calculated on a dry basis, and the polysaccharide content in the snow lotus culture is 8%; the astragaloside A content in the astragalus is 0.8%.

[0051] This embodiment prepares a sports nutrition food in powder form, with an energy value of 1400kJ, a carbohydrate content of 70g, and a branched-chain amino acid content of 3g per 100g of product.

[0052] The preparation method of the above-mentioned sports nutrition food with the function of clearing turbidity and dissolving stones includes the following steps:

[0053] S1. Raw material pretreatment: Ginseng, Astragalus, snow lotus culture, longan, red dates, dandelion, houttuynia cordata, corn silk, chicken gizzard lining and Polygonatum sibiricum are crushed to a particle size of 0.5mm and set aside.

[0054] S2, Water extraction: Mix the pretreated raw materials from S1, add purified water, the mass ratio of purified water to mixed raw materials is 10:1, extract at 87℃ for 2 hours to obtain the extract;

[0055] The extraction process was carried out in two stages. The first extraction was carried out at 87℃ for 1.5 hours, and the second extraction was carried out at 92℃ for 1 hour. The two extracts were combined, and 0.2% by weight of disodium ethylenediaminetetraacetate was added as a metal ion chelating agent during the second extraction.

[0056] S3. Enzymatic hydrolysis: Add a complex enzyme, which includes cellulase and protease, to the extract obtained in S2. The amount added is 0.3% of the weight of the extract. Hydrolyze for 1.5 h at pH 5.75 and temperature 50℃, and then heat to 85℃ and hold for 10 min to inactivate the enzyme to obtain the hydrolysate.

[0057] The mass ratio of cellulase to protease in the complex enzyme is 1.5:1, and it is pretreated with ultrasound before enzymatic hydrolysis. The ultrasound frequency is 30kHz and the treatment time is 10min.

[0058] S4. Fermentation enhancement: The enzymatic hydrolysate obtained in S3 was inoculated with probiotics Lactobacillus at an inoculation amount of 2% of the weight of the enzymatic hydrolysate. Fermentation was carried out at 37℃ for 18 hours. After fermentation, the temperature was heated to 70℃ and held for 5 minutes to terminate fermentation, and the fermentation broth was obtained.

[0059] Among them, the probiotic lactobacillus is Lactobacillus plantarum, with a live count of not less than 1×10⁻⁶. 9 CFU / g, and 1% of the fermentation liquid weight of fructooligosaccharides is added as a prebiotic during the fermentation process;

[0060] S5. Filtration and Concentration: The fermentation broth obtained in S4 is filtered through a 150-mesh sieve to remove residue and obtain filtrate; the filtrate is concentrated at a vacuum of 0.075 MPa and a temperature of 60°C until the solid content is 50% to obtain concentrated liquid.

[0061] S6. Mixing and homogenization: Add the above-mentioned parts by weight of soybean peptides, ginger powder, honey, apple juice, citric acid, walnut oil, potassium sorbate, branched-chain amino acids, fructooligosaccharides, and microencapsulated vitamin B1 and vitamin B2 to the concentrate obtained in S5. Mix at a stirring speed of 350 r / min for 20 min, and then homogenize twice using a homogenizer at a pressure of 30 MPa to obtain a homogenized mixture.

[0062] Among them, the microencapsulated vitamin B1 and vitamin B2 were prepared by the following method: sodium alginate was prepared into a 4% solution, vitamin B1 and vitamin B2 were added, and microcapsules were prepared by spray drying at an inlet temperature of 170℃ and an outlet temperature of 80℃ to obtain microencapsulated vitamin B1 and vitamin B2.

[0063] S7. Molding and Packaging: The homogeneous mixture obtained in S6 is spray-dried at an inlet air temperature of 165°C and an outlet air temperature of 80°C to obtain powder, which is then compressed into tablets.

[0064] In this process, maltodextrin is added as a carrier before spray drying, and the mass ratio of maltodextrin to the homogenized mixture is 1:7.5.

[0065] Example 2

[0066] This application provides a sports nutrition food with the function of clearing turbidity and dissolving stones, which contains the following ingredients by weight:

[0067] Ginseng 3 parts, Astragalus 8 parts, Snow Lotus culture 3 parts, Soybean peptide 6 parts, Longan 4 parts, Red dates 7 parts, Dandelion 2 parts, Houttuynia cordata 2 parts, Corn silk 2 parts, Chicken gizzard lining 2 parts, Ginger powder 1 part, Honey 5 parts, Apple juice 11 parts, Polygonatum sibiricum 4 parts, Citric acid 1 part, Walnut oil 1 part, Potassium sorbate 0.1 parts, Branched-chain amino acids 3 parts, Fructooligosaccharides 6 parts, Vitamin B1 0.5 parts and Vitamin B2 0.2 parts.

[0068] Among them, the branched-chain amino acids include leucine, isoleucine and valine, with a weight ratio of 2.0:0.8:0.8; vitamin B1 and vitamin B2 exist in microencapsulated form, with sodium alginate as the microcapsule wall material and an encapsulation rate of not less than 85%.

[0069] Among them, the ginseng is artificially cultivated 4-year-old ginseng, and the total content of ginsenosides Rg1 and Rb1 in the ginseng is 1.5%; the snow lotus culture is calculated on a dry basis, and the polysaccharide content in the snow lotus culture is 8%; the astragaloside A content in the astragalus is 0.8%.

[0070] This embodiment prepares a liquid sports nutrition food with an energy value of 1000kJ, a carbohydrate content of 10g, and a branched-chain amino acid content of 1g per 100mL.

[0071] The preparation method of the above-mentioned sports nutrition food with the function of clearing turbidity and dissolving stones includes the following steps:

[0072] S1. Raw material pretreatment: Ginseng, Astragalus, snow lotus culture, longan, red dates, dandelion, houttuynia cordata, corn silk, chicken gizzard lining and Polygonatum sibiricum are crushed to a particle size of 0.1mm and set aside.

[0073] S2, Water extraction: Mix the pretreated raw materials from S1, add purified water, the mass ratio of purified water to mixed raw materials is 8:1, extract at 80℃ for 1 hour to obtain the extract;

[0074] S3. Enzymatic hydrolysis: Add a complex enzyme, which includes cellulase and protease, to the extract obtained in S2. The amount added is 0.1% of the weight of the extract. Hydrolyze at pH 5.0 and 45℃ for 1 hour, then raise the temperature to 85℃ and hold for 10 minutes to inactivate the enzyme, and obtain the hydrolysate.

[0075] S4. Fermentation enhancement: The enzymatic hydrolysate obtained in S3 was inoculated with probiotics Lactobacillus at a rate of 1% of the weight of the enzymatic hydrolysate. Fermentation was carried out at 35°C for 12 hours. After fermentation, the temperature was heated to 70°C and maintained for 5 minutes to terminate the fermentation, and the fermentation broth was obtained.

[0076] S5. Filtration and Concentration: The fermentation broth obtained in S4 is filtered through a 100-mesh sieve to remove residue and obtain filtrate; the filtrate is concentrated at a vacuum of 0.06 MPa and a temperature of 50°C until the solid content is 40% to obtain concentrated liquid.

[0077] S6. Mixing and homogenization: Add the above-mentioned parts by weight of soybean peptides, ginger powder, honey, apple juice, citric acid, walnut oil, potassium sorbate, branched-chain amino acids, fructooligosaccharides, and microencapsulated vitamin B1 and vitamin B2 to the concentrate obtained in S5. Mix at a stirring speed of 200 r / min for 10 min, and then homogenize once using a homogenizer at a pressure of 20 MPa to obtain a homogenized mixture.

[0078] Among them, the microencapsulated vitamin B1 and vitamin B2 were prepared by the following method: sodium alginate was prepared into a 3% solution, vitamin B1 and vitamin B2 were added, and microcapsules were prepared by spray drying at an inlet temperature of 160℃ and an outlet temperature of 70℃ to obtain microencapsulated vitamin B1 and vitamin B2.

[0079] S7. Molding and Packaging: The homogeneous mixture obtained in S6 is directly filled and sealed.

[0080] Example 3

[0081] This application provides a sports nutrition food with the function of clearing turbidity and dissolving stones, which contains the following ingredients by weight:

[0082] 5 parts ginseng, 10 parts astragalus, 5 parts snow lotus culture, 8 parts soybean peptide, 6 parts longan, 9 parts red dates, 4 parts dandelion, 4 parts houttuynia cordata, 3 parts corn silk, 3 parts chicken gizzard lining, 3 parts ginger powder, 7 parts honey, 15 parts apple juice, 6 parts polygonatum, 2 parts citric acid, 2 parts walnut oil, 0.3 parts potassium sorbate, 5 parts branched-chain amino acids, 8 parts fructooligosaccharides, 11 parts vitamin B1 and 0.5 parts vitamin B2.

[0083] Among them, the branched-chain amino acids include leucine, isoleucine and valine, with a weight ratio of 2.5:1.2:1.2; vitamin B1 and vitamin B2 exist in microencapsulated form, with sodium alginate as the microcapsule wall material and an encapsulation rate of not less than 85%.

[0084] The ginseng is artificially cultivated 5-year-old ginseng, and the total content of ginsenosides Rg1 and Rb1 in the ginseng is 1.5%; the snow lotus culture is calculated on a dry basis, and the polysaccharide content in the snow lotus culture is 8%; the astragaloside A content in the astragalus is 0.8%.

[0085] This embodiment prepares a sports nutrition food in pill form, with an energy value of 1600kJ, a carbohydrate content of 75g, and a branched-chain amino acid content of 4g per 100g.

[0086] The preparation method of the above-mentioned sports nutrition food with the function of clearing turbidity and dissolving stones includes the following steps:

[0087] S1. Raw material pretreatment: Ginseng, Astragalus, snow lotus culture, longan, red dates, dandelion, houttuynia cordata, corn silk, chicken gizzard lining and Polygonatum sibiricum are crushed to a particle size of 1.0 mm and set aside.

[0088] S2, Water extraction: Mix the pretreated raw materials from S1, add purified water, the mass ratio of purified water to mixed raw materials is 12:1, extract at 95℃ for 3 hours to obtain the extract;

[0089] The extraction process is carried out in two stages. The first extraction is carried out at 90℃ for 2 hours, and the second extraction is carried out at 95℃ for 1.5 hours. The two extracts are combined, and 0.3% by weight of disodium ethylenediaminetetraacetate is added as a metal ion chelating agent during the second extraction.

[0090] S3. Enzymatic hydrolysis: Add a complex enzyme, which includes cellulase and protease, to the extract obtained in S2. The amount added is 0.5% of the weight of the extract. Hydrolyze the extract at pH 6.5 and 55℃ for 2 hours. Then, heat the extract to 85℃ and hold for 10 minutes to inactivate the enzyme, and obtain the hydrolysate.

[0091] The mass ratio of cellulase to protease in the complex enzyme is 2:1, and it is pretreated with ultrasound before enzymatic hydrolysis. The ultrasound frequency is 40kHz and the treatment time is 15min.

[0092] S4. Fermentation enhancement: The enzymatic hydrolysate obtained in S3 was inoculated with probiotics Lactobacillus at a rate of 3% of the weight of the enzymatic hydrolysate. Fermentation was carried out at 40℃ for 24 hours. After fermentation, the temperature was heated to 70℃ and held for 5 minutes to terminate fermentation, and the fermentation broth was obtained.

[0093] S5. Filtration and Concentration: The fermentation broth obtained in S4 is filtered through a 200-mesh sieve to remove residue and obtain filtrate; the filtrate is concentrated at a vacuum of 0.09 MPa and a temperature of 70°C until the solid content is 60% to obtain concentrated liquid.

[0094] S6. Mixing and homogenization: Add the above-mentioned parts by weight of soybean peptides, ginger powder, honey, apple juice, citric acid, walnut oil, potassium sorbate, branched-chain amino acids, fructooligosaccharides, and microencapsulated vitamin B1 and vitamin B2 to the concentrate obtained in S5. Mix at a stirring speed of 500 r / min for 30 min, and then homogenize three times under a pressure of 40 MPa to obtain a homogenized mixture.

[0095] Among them, the microencapsulated vitamin B1 and vitamin B2 were prepared by the following method: sodium alginate was prepared into a 5% solution, vitamin B1 and vitamin B2 were added, and microcapsules were prepared by spray drying at an inlet temperature of 180℃ and an outlet temperature of 85℃ to obtain microencapsulated vitamin B1 and vitamin B2.

[0096] S7. Molding and Packaging: The homogeneous mixture obtained in S6 is granulated after adding excipients and dried at 60°C until the moisture content is less than 5%, and then shaped into pills.

[0097] The added excipients include microcrystalline cellulose and magnesium stearate. The amount of microcrystalline cellulose added is 5% of the total mass of the pills, and the amount of magnesium stearate added is 1.5% of the total mass of the pills. After the pills are formed, fluidized bed coating technology is used for coating. The coating material is hydroxypropyl methylcellulose, and the weight gain of the coating is 5%.

[0098] Experiment 1: Bioavailability Test of Branched-Chain Amino Acids

[0099] The testing standards were based on GB28050-2011 "National Food Safety Standard - General Rules for Nutrition Labeling of Prepackaged Foods" and the bioavailability determination method in "Technical Specifications for Inspection and Evaluation of Health Foods". Eighty healthy male subjects aged 18-30 years were randomly divided into four groups of 20 each, corresponding to Examples 1-3 and the comparative example, respectively. The comparative example was a mainstream sports nutrition drink on the market, whose core ingredients are branched-chain amino acids, glucose, vitamin B1, and vitamin B2. It does not contain traditional Chinese medicine extracts, probiotics, or fructooligosaccharides. Each 100mL contains 950kJ of energy, 12g of carbohydrates, 1.2g of branched-chain amino acids, 0.4g of vitamin B1, and 0.2g of vitamin B2. The recommended daily dose is 100mL per serving.

[0100] Subjects fasted for 12 hours prior to the trial. On the day of the trial, the sample from Example 1 was taken after being dissolved in 500 mL of warm water at a dose of 100 g; the sample from Example 2 was taken directly at a dose of 100 mL per person; the sample from Example 3 was taken after being dissolved in 500 mL of warm water at a dose of 100 g per person; and the comparative sample was taken at the recommended dose of 100 mL per person. 5 mL of venous blood was collected from subjects at 0 hours before administration and at 0.5, 1, 2, 3, 4, and 5 hours after administration. After centrifugation to separate the serum, the concentrations of leucine, isoleucine, and valine in the serum were determined using high-performance liquid chromatography (HPLC). Blood drug concentration-time curves were plotted, and the area under the curve was calculated. Using the AUC of the comparative sample as a reference, the relative bioavailability of the branched-chain amino acids in each example was calculated as: Relative bioavailability = (average AUC of each example / average AUC of the comparative sample) × 100%. The difference in bioavailability between each example and the comparative sample was compared to reflect the absorption and utilization efficiency of the branched-chain amino acids in the product.

[0101] Experiment 2: Lactic acid clearance rate and energy replenishment efficiency test

[0102] The testing standards followed the energy replenishment and fatigue recovery testing methods in GB24154-2015 "General Rules for Sports Nutrition Foods". Eighty healthy male athletes aged 18-30 were randomly divided into four groups of 20 each, corresponding to Examples 1-3 and the comparative example. The comparative example used the same mainstream sports nutrition drinks as described in Experiment 1, with consistent core parameters.

[0103] Subjects underwent a uniform load exercise on a stationary bicycle at a constant temperature of 25°C for 60 minutes, with an intensity of 70% of their maximum oxygen uptake. Immediately after exercise, 5 mL of venous blood was collected to determine the initial blood lactate and blood glucose concentrations. Subsequently, each group ingested the corresponding sample at the same dosage as in Experiment 1. Venous blood was collected again at 30, 60, 90, and 120 minutes after ingestion. Blood lactate levels were measured using the lactate oxidase method, and blood glucose levels were measured using the glucose oxidase method. The lactate clearance rate at each time point was calculated as: Lactate clearance rate = (Initial lactate concentration - Lactate concentration at each time point) / Initial lactate concentration × 100%; the blood glucose recovery rate was calculated as: Blood glucose recovery rate = (Blood glucose concentration at each time point - Blood glucose concentration immediately after exercise) / Time × 100%. The lactate clearance rate and blood glucose recovery rate at different time points were compared between the examples and the comparative examples to reflect the product's effectiveness in post-exercise fatigue recovery and energy replenishment.

[0104] Experiment 3: Intestinal Tolerance and Probiotic Survival Stability Test

[0105] The testing standards were based on the "Technical Specifications for Inspection and Evaluation of Probiotic Health Foods" and the intestinal tolerance test method in GB17399-2016 "National Food Safety Standard for Candy". First, the number of viable probiotics in the samples was detected. The comparative example was the mainstream sports nutrition drink described in Experiment 1, which does not contain probiotics; therefore, the initial number of viable probiotics was calculated as 0 CFU / g. 10g of each sample from Examples 1-3 and 10mL of the comparative example sample were taken, and the number of viable lactic acid bacteria was determined using the plate count method according to GB4789.35-2016 "National Food Safety Standard for Microbiological Examination of Food - Lactic Acid Bacteria Examination". Subsequently, a human intestinal tolerance test was conducted. Eighty healthy subjects aged 18-30 years were randomly divided into four groups of 20 each, corresponding to the examples and comparative examples, respectively. The subjects took the samples continuously for 7 days, with the daily dosage consistent with Experiment 1. Daily gastrointestinal reactions, including bloating, diarrhea, abdominal pain, nausea, and other discomfort, were recorded. The incidence of discomfort in each group was calculated as follows: Incidence of discomfort = (Number of people experiencing discomfort / Total number of people in the group) × 100%. Finally, a simulated gastrointestinal environment survival test was conducted. Appropriate amounts of each sample were added to simulated gastric fluid for 2 hours, followed by treatment with simulated intestinal fluid for 4 hours. After treatment, the number of surviving lactic acid bacteria was determined using the plate count method, and the survival rate was calculated as follows: Survival rate = (Number of surviving bacteria after treatment / Initial number of surviving bacteria) × 100%. Comparison of the initial number of surviving probiotics, the incidence of gastrointestinal discomfort, and the survival rate after simulating the gastrointestinal environment in each example and comparative example reflects the product's intestinal tolerance and the stability of probiotic survival.

[0106] Experiment 4: Test of the efficacy of clearing turbidity and dissolving stones

[0107] The testing standards refer to the relevant efficacy evaluation methods in the "Technical Specifications for Inspection and Evaluation of Health Foods", and adopt a combination of simulated urine environment stone dissolution test and animal experiment.

[0108] Simulated urine environment stone dissolution test: Artificial urine with pH 6.0-6.5 was prepared, and an equal amount of synthetic calcium oxalate stone particles with a particle size of 0.1-0.3 mm were added. Extracts from the samples of Examples 1-3 and the comparative sample were added, while the control group consisted of artificial urine without samples. The mixture was incubated at 37℃ and 100 rpm for 24 hours in a constant temperature shaking incubator. The calcium ion concentration in the solution was determined by inductively coupled plasma mass spectrometry (ICP-MS), and the stone dissolution rate was calculated as: Stone dissolution rate = (Calcium ion concentration in the experimental group - Calcium ion concentration in the control group) / (Calcium ion concentration in the completely dissolved stone - Calcium ion concentration in the control group) × 100%. Animal experiment: Sixty male SD rats were randomly divided into four groups of 15 each: the Example 1-3 group, the comparative group, and the model control group. Except for the normal control group, urinary system stone models were established in all other groups using the ethylene glycol-ammonium chloride method. After successful modeling, groups 1-3 were administered the corresponding samples by gavage at the recommended dose, the control group was administered the corresponding control sample by gavage, and the model control group was administered an equal volume of physiological saline by gavage. This gavage was continued for 21 consecutive days. After the last gavage, 24-hour urine samples were collected from the rats, and the concentrations of oxalate, phosphate, and calcium ions in the urine were measured. The rats were dissected to observe the formation of kidney and ureteral stones, calculate the stone incidence rate, and weigh the wet stones. Pathological changes in kidney tissue were also examined. The stone dissolution rate, urinary metabolic indicators, stone incidence rate, and wet stone weight of each example and control group were compared to reflect the product's efficacy in clearing turbidity and dissolving stones.

[0109] The bioavailability test data of branched-chain amino acids are shown in Table 1.

[0110] Table 1:

[0111] Group AUC (leucine) (μg·h / mL) AUC (Isoleucine) (μg·h / mL) AUC (valine) (μg·h / mL) Total AUC (μg·h / mL) Relative bioavailability (%) Example 1 45.2±2.1 38.7±1.8 36.9±1.5 120.8±4.2 158.3±5.2 Example 2 42.8±1.9 36.5±1.6 35.2±1.4 114.5±3.8 150.1±4.7 Example 3 43.9±2.0 37.8±1.7 36.1±1.5 117.8±4.0 154.5±5.0 Comparative Example 28.5±1.2 24.3±1.0 23.8±0.9 76.3±2.5 100.0±3.1

[0112] The lactic acid clearance rate at different time points is shown in Table 2 (%).

[0113] Table 2:

[0114] Group 30 minutes 60 minutes 90 minutes 120 minutes Example 1 25.3±2.1 48.7±3.2 72.5±4.1 88.9±4.8 Example 2 23.8±1.9 45.2±2.9 68.7±3.8 85.3±4.5 Example 3 24.6±2.0 47.1±3.0 70.8±4.0 87.2±4.6 Comparative Example 18.5±1.5 35.8±2.4 56.3±3.2 72.6±3.9

[0115] The blood glucose recovery rate (mg / dL·min) is shown in Table 3.

[0116] Table 3:

[0117] Group 30 minutes 60 minutes 90 minutes 120 minutes Example 1 1.85±0.15 1.52±0.12 1.18±0.09 0.85±0.07 Example 2 1.72±0.13 1.43±0.11 1.09±0.08 0.78±0.06 Example 3 1.78±0.14 1.48±0.11 1.14±0.09 0.82±0.07 Comparative Example 1.35±0.10 1.12±0.09 0.86±0.07 0.62±0.05

[0118] The survival status of probiotics is shown in Table 4.

[0119] Group Initial viable count (CFU / g) Survival count after artificial gastric fluid treatment (CFU / g) Survival count after artificial intestinal fluid treatment (CFU / g) Overall survival rate (%) Example 1 (3.2 ± 0.3) x 10 8 ]] <![CDATA[(2.8±0.2)×10 8 ]]> <![CDATA[(2.5±0.2)×10 8 ]]> 78.1±5.2 Example 2 <![CDATA[(2.8±0.2)×10 8 ]]> <![CDATA[(2.4±0.2)×10 8 ]]> <![CDATA[(2.1±0.2)×10 8 ]]> 75.0±4.8 Example 3 <![CDATA[(3.0±0.3)×10 8 ]]> <![CDATA[(2.6±0.2)×10 8 ]]> <![CDATA[(2.3±0.2)×10 8 ]]> 76.7±5.0 Comparative Example 0 0 0 0

[0120] Intestinal tolerance is shown in Table 5.

[0121] Table 5:

[0122] Group Sample size Incidence of abdominal distension (%) Diarrhea incidence (%) Incidence of abdominal pain (%) Incidence of nausea (%) Overall incidence of discomfort (%) Example 1 20 5 0 5 0 10 Example 2 20 5 0 0 5 10 Example 3 20 0 5 5 0 10 Comparative Example 20 15 10 15 10 30

[0123] The test data on the efficacy of clearing turbidity and dissolving stones are shown in Table 6.

[0124] Table 6:

[0125] Group Simulated urine stone dissolution rate (%) Incidence of stones in rats (%) Average wet weight of gallstones (mg) Urinary oxalate concentration (mmol / L) Urinary calcium ion concentration (mmol / L) Example 1 32.5±3.1 20.0 4.2±0.8 1.2±0.2 2.8±0.3 Example 2 28.3±2.8 33.3 5.7±1.0 1.5±0.3 2.5±0.2 Example 3 30.7±3.0 26.7 4.9±0.9 1.3±0.2 2.6±0.3 Comparative Example 8.5±1.5 73.3 12.5±1.5 2.8±0.4 1.6±0.2 Model control group 5.2±1.2 86.7 15.3±1.8 3.2±0.5 1.4±0.2

[0126] The experimental analysis is as follows:

[0127] Combining Examples 1-3 and the comparative examples with Table 1, it can be seen that the design of the branched-chain amino acid (BCAA) ratio, the addition of traditional Chinese medicine extracts, and the combination of enzymatic hydrolysis and fermentation processes significantly affect the bioavailability of BCAAs. There is a synergistic effect between the active ingredients in the traditional Chinese medicine extracts and soybean peptides, which can help promote the intestinal absorption of amino acids. Furthermore, the specific ratio of leucine, isoleucine, and valine better meets the metabolic needs of the human body. In addition, the degradation effect of raw material components by enzymatic hydrolysis and the optimization of nutrient conversion during fermentation collectively promote the improved absorption efficiency of BCAAs in vivo. The comparative examples, containing only basic BCAAs and simple carbohydrates and lacking traditional Chinese medicine extracts, probiotics, and related optimized processes, also demonstrate the positive role of these additional ingredients and processes in promoting nutrient absorption.

[0128] Combining Examples 1-3 and the comparative example with Table 2, it can be seen that the change in lactic acid clearance rate is closely related to the synergistic effect of the traditional Chinese medicine extracts, branched-chain amino acids, and probiotics in the formula. The active ingredients in the extracts of traditional Chinese medicines such as ginseng and astragalus can regulate the activity of metabolic enzymes in the body; branched-chain amino acids can participate in muscle energy metabolism and reduce lactic acid accumulation; and probiotics indirectly assist in the excretion of metabolic waste by regulating the balance of intestinal flora. The combined effect of these components accelerates the clearance of lactic acid after exercise. The comparative example only contains basic energy substances and branched-chain amino acids, lacking the synergistic support of traditional Chinese medicine extracts and probiotics. Its trend in lactic acid clearance further illustrates that the rational combination of multiple functional components can more efficiently promote the metabolism of fatigue-related metabolites after exercise.

[0129] Combining Examples 1-3 and the comparative examples with Table 3, it can be seen that the difference in blood glucose recovery rate mainly stems from the rational combination of nutritional components and the absorption-promoting mechanism. The carbohydrates, honey, and apple juice in the formula provide rapidly absorbable energy substances, which work synergistically with the active ingredients in the herbal extracts that aid energy conversion. Furthermore, the B vitamins' auxiliary effect on energy metabolism further promotes a rapid recovery of blood glucose after exercise. The comparative examples, with their relatively singular energy source and lacking the synergistic effect of the herbal extracts, demonstrate that the combination of diverse energy components and metabolic regulatory components is more conducive to meeting the body's immediate and sustained energy replenishment needs after exercise.

[0130] Combining Examples 1-3 and the comparative examples with Table 4, it can be seen that the survival stability of probiotics depends on the combined effects of inoculation process, prebiotic addition, and microencapsulation protection technology. Targeted inoculation of *Lactobacillus plantarum* provides the product with sufficient probiotics, fructooligosaccharides, as prebiotics, provide nutritional support for probiotic growth, and sodium alginate microencapsulation effectively protects probiotics from the damage caused by the acidic and alkaline environment of the gastrointestinal tract. Furthermore, processes such as fermentation and homogenization enhance the stability of the product system, collectively ensuring the survival ability of probiotics in vivo. The comparative examples, which did not contain added probiotics and related protective components, show a clear difference in survival compared to the examples, demonstrating that effective retention of probiotics requires the synergistic effect of multiple technologies and components, including inoculation, protection, and nutrient supply.

[0131] Combining Examples 1-3 and the comparative examples with Table 5, it can be seen that the quality of intestinal tolerance is related to the mildness of the formula ingredients, the balance of probiotics and prebiotics, and the refinement of the processing. The mild conditioning effects of ingredients such as longan and jujube in the formula, combined with the regulation of intestinal flora by probiotics and the intestinal protective effect of fructooligosaccharides, can reduce the occurrence of gastrointestinal discomfort. Homogenization and other processing methods further improve the mildness and compatibility of the ingredients. The difference in the occurrence of intestinal discomfort in the comparative examples, which lack these conditioning ingredients and process optimization, indicates that the intestinal compatibility of sports nutrition foods not only requires avoiding irritating ingredients but also necessitates the synergistic conditioning of multiple ingredients and process optimization to reduce the burden on the gastrointestinal tract from long-term consumption.

[0132] As can be seen from Examples 1-3 and the comparative examples, and in conjunction with Table 6, the differences in the efficacy of clearing turbidity and dissolving stones stem from the synergistic effect of the clearing turbidity and dissolving stone components such as dandelion, houttuynia cordata, corn silk, and chicken gizzard lining, as well as the preparation process ensuring their activity. The active substances in these components can regulate urine metabolism, reduce the concentration of substances related to stone formation, and promote the dissolution and excretion of small stones. Stepwise extraction and enzymatic hydrolysis processes ensure the full release and preservation of the activity of these components. The comparative examples, lacking the clearing turbidity and dissolving stone components, show significant differences in stone dissolution rate and stone incidence compared to the examples. This trend indicates that the realization of the clearing turbidity and dissolving stone function requires the scientific formulation of specific active ingredients and the synergistic effect of appropriate processes, which can effectively help athletes reduce the deposition of toxins in their bodies and lower the risk of stone formation.

[0133] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A sports nutrition food with the function of clearing turbidity and dissolving stones, characterized in that: The product contains the following ingredients by weight: ginseng 3-5 parts, astragalus 8-10 parts, snow lotus culture 3-5 parts, soybean peptide 6-8 parts, longan 4-6 parts, red dates 7-9 parts, dandelion 2-4 parts, houttuynia cordata 2-4 parts, corn silk 2-3 parts, chicken gizzard lining 2-3 parts, ginger powder 1-3 parts, honey 5-7 parts, apple juice 11-15 parts, polygonatum 4-6 parts, citric acid 1-2 parts, walnut oil 1-2 parts, potassium sorbate 0.1-0.3 parts, branched-chain amino acids 3-5 parts, fructooligosaccharides 6-8 parts, vitamin B1 0.5-1 part, and vitamin B2 0.2-0.5 parts.

2. A sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 1, characterized in that: The branched-chain amino acids include leucine, isoleucine and valine, in a weight ratio of (2.0-2.5):(0.8-1.2):(0.8-1.2); the vitamin B1 and vitamin B2 are present in microencapsulated form, with sodium alginate as the microcapsule wall material and an encapsulation rate of not less than 85%.

3. A sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 1, characterized in that: The ginseng is artificially cultivated 4-5 year old ginseng, and the total content of ginsenosides Rg1 and Rb1 in the ginseng is not less than 1.5%; the snow lotus culture is calculated on a dry basis, and the polysaccharide content in the snow lotus culture is not less than 8%; the astragaloside A content in the astragalus is not less than 0.8%.

4. A sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 1, characterized in that: The sports nutrition food is in the form of liquid, powder, or pills. When the product is in liquid form, the energy value per 100mL is 1000-1500kJ, the carbohydrate content is 10-20g, and the branched-chain amino acid content is 1-3g. When the product is in the form of powder or pills, the energy value per 100g is 1200-1600kJ, the carbohydrate content is 65-75g, and the branched-chain amino acid content is 2-4g.

5. A method for preparing a sports nutrition food with the function of clearing turbidity and dissolving stones, characterized in that, A sports nutrition food with the function of clearing turbidity and dissolving stones as described in any one of claims 1-4 includes the following steps: S1. Raw material pretreatment: Ginseng, Astragalus, snow lotus culture, longan, red dates, dandelion, houttuynia cordata, corn silk, chicken gizzard lining and Polygonatum sibiricum are crushed into particles with a diameter of 0.1-1.0 mm and set aside. S2, Water extraction: Mix the pretreated raw materials in S1, add purified water, and the mass ratio of purified water to mixed raw materials is 8:1 to 12:

1. Extract at a temperature of 80-95℃ for 1-3 hours to obtain the extract. S3. Enzymatic hydrolysis: Add a complex enzyme, including cellulase and protease, to the extract obtained in S2. The amount added is 0.1-0.5% of the weight of the extract. Hydrolyze for 1-2 hours at pH 5.0-6.5 and temperature 45-55℃. Then raise the temperature to 85℃ and hold for 10 minutes to inactivate the enzyme, and obtain the hydrolysate. S4. Fermentation enhancement: Inoculate the enzymatic hydrolysate obtained in S3 with probiotics Lactobacillus, with an inoculation amount of 1-3% of the weight of the enzymatic hydrolysate. Ferment at 35-40℃ for 12-24 hours. After fermentation, heat to 70℃ and maintain for 5 minutes to terminate fermentation and obtain fermentation broth. S5. Filtration and Concentration: Filter the fermentation broth obtained in S4 through a 100-200 mesh sieve to remove residue and obtain filtrate; concentrate the filtrate under a vacuum of 0.06-0.09 MPa and a temperature of 50-70℃ until the solid content is 40-60% to obtain concentrated liquid. S6. Mixing and homogenization: Add the soybean peptides, ginger powder, honey, apple juice, citric acid, walnut oil, potassium sorbate, branched-chain amino acids, fructooligosaccharides, and microencapsulated vitamin B1 and vitamin B2 to the concentrate obtained in S5. Mix at a stirring speed of 200-500 r / min for 10-30 min, and then homogenize 1-3 times using a homogenizer at a pressure of 20-40 MPa to obtain a homogenized mixture. S7. Molding and Packaging: The homogeneous mixture obtained in S6 is processed according to the product form. For liquid forms, fill directly and seal; For powder form, spray drying is performed with an inlet air temperature of 150-180℃ and an outlet air temperature of 70-90℃ to obtain powder, which is then compressed into tablets or filled into capsules. For pill form, excipients are added, granulation is performed, and the mixture is dried at a temperature of 40-60℃ until the moisture content is less than 5%, and then formed into pills.

6. The method for preparing a sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 5, characterized in that: In step S2, the extraction process is carried out in two stages. The first extraction temperature is 85-90℃ and the time is 1-2 hours. The second extraction temperature is 90-95℃ and the time is 0.5-1.5 hours. The two extracts are combined. During the second extraction, 0.1-0.3% of the weight of the extract disodium ethylenediaminetetraacetate is added as a metal ion chelating agent.

7. The preparation method of a sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 5, characterized in that: In step S3, the mass ratio of cellulase to protease in the complex enzyme is 1:1 to 2:1, and it is pretreated with ultrasound before enzymatic hydrolysis. The ultrasound frequency is 20-40 kHz and the treatment time is 5-15 min.

8. The method for preparing a sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 5, characterized in that: In step S4, the probiotic lactobacillus is Lactobacillus plantarum, with a live count of not less than 1×10⁻⁶. 9 The concentration is CFU / g, and fructooligosaccharides accounting for 0.5-1.5% of the fermentation liquid weight are added as prebiotics during the fermentation process.

9. A method for preparing a sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 5, characterized in that: In step S6, the microencapsulated vitamin B1 and vitamin B2 are prepared by the following method: sodium alginate is prepared into a 3-5% solution, vitamin B1 and vitamin B2 are added, and microcapsules are prepared by spray drying at an inlet temperature of 160-180℃ and an outlet temperature of 70-85℃ to obtain microencapsulated vitamin B1 and vitamin B2.

10. A method for preparing a sports nutrition food with the function of clearing turbidity and dissolving stones according to claim 5, characterized in that: In step S7, for powder form, maltodextrin is added as a carrier before spray drying, and the mass ratio of maltodextrin to homogenized mixture is 1:5 to 1:10; for pellet form, the added excipients include microcrystalline cellulose and magnesium stearate, the amount of microcrystalline cellulose added is 2-5% of the total mass of pellets, the amount of magnesium stearate added is 0.5-1.5% of the total mass of pellets, and after pellet formation, fluidized bed coating technology is used for coating, the coating material is hydroxypropyl methylcellulose, and the coating weight gain is 2-5%.

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