Bio-organic fertilizer with edible fungus residues as main material as well as preparation method and application of bio-organic fertilizer
By combining a compound microbial agent of Bacillus saccharophilus, Sphingomyelinus hydrophila, and Acinetobacter guangzhou with functionalized biochar, the problem of incomplete composting of edible mushroom residue was solved, soil fertility and tomato yield and quality were improved, and the resource utilization of edible mushroom residue was realized.
Patent Information
- Application Number
- CN202511508156.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-22
- Publication Date
- 2026-01-09
AI Technical Summary
Traditional edible mushroom residue composting is not fully decomposed, has a single type of functional microorganism, and has a slow fertilizer effect, leading to soil compaction and nutrient imbalance, which affects tomato yield and quality.
Bio-organic fertilizer is prepared by combining a compound microbial agent of Bacillus saccharophilus, Sphingomyelinus hydrophila, and Acinetobacter guangzhou with functionalized biochar and mineral activators. The synergistic effect of the three agents accelerates the decomposition of edible fungi residue, improves soil fertility, and promotes tomato growth.
It significantly improves soil physical and chemical properties, increases tomato yield and quality, realizes the resource utilization of edible mushroom residue, and reduces environmental pollution.
Smart Images
Figure CN121293043A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of bio-organic fertilizer technology, specifically relating to a bio-organic fertilizer with edible mushroom residue as the main material, its preparation method, and its application. Background Technology
[0002] The yield and value of edible fungi are gradually increasing, continuously developing into an important economic crop. However, mushroom residue is mostly disposed of through incineration, discarding, or direct return to the field, leading to environmental pollution and resource waste. Mushroom residue contains a large amount of organic components, rich in protein, amino acids, and various mineral elements. After composting and fermentation, it possesses unique physicochemical properties and abundant nutrients, making it an ideal raw material for preparing organic fertilizer. Bio-organic fertilizer is increasingly widely used in agricultural production due to its environmental friendliness, ease of use, and abundant raw materials. The main component of mushroom residue is the culture medium left after harvesting edible fungi, typically containing complex organic matter that is difficult to decompose, such as lignin, cellulose, and hemicellulose, as well as unused nitrogen sources and other nutrients. Using mushroom residue as the main raw material to prepare bio-organic fertilizer can achieve "turning waste into treasure." However, traditional mushroom residue composting generally suffers from incomplete composting, limited functional microorganisms, and slow fertilizer effects, restricting its application. The combination of suitable strains can greatly accelerate the composting process of mushroom residue and transform it into high-quality organic fertilizer.
[0003] Tomatoes are mostly grown in soil and heavily fertilized with chemical fertilizers. Continuous cropping year after year can easily lead to severe soil compaction, nutrient imbalance, weakened soil fertility, and increased soil-borne diseases, ultimately affecting quality and reducing yield. Therefore, developing a new type of fertilizer that can realize the resource utilization of agricultural waste and simultaneously improve soil and enhance crop quality is of significant practical importance. Summary of the Invention
[0004] The purpose of this invention is to provide a bio-organic fertilizer with edible mushroom residue as the main material, which can effectively improve the physical and chemical properties of the soil and significantly increase the yield and quality of tomatoes.
[0005] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows: A bio-organic fertilizer with edible mushroom residue as the main ingredient is characterized by comprising the following raw materials in parts by weight: 60-75 parts edible mushroom residue, 15-25 parts decomposed poultry and livestock manure, 5-8 parts humic acid, 3-5 parts compound microbial agent, 0.5-1.5 parts polyglutamic acid, 3-5 parts mineral activator, 2-4 parts seaweed extract, and 3-6 parts functionalized biochar.
[0006] Furthermore, the edible mushroom residue is oyster mushroom residue.
[0007] Furthermore, the compound microbial agent includes *Bacillus saccharophilus*, *Sphingomonas hydrate*, and *Acinetobacter guangzhouensis*. The *Bacillus saccharophilus* was purchased from the China General Microbiological Culture Collection Center (CGMCC), strain number CGMCC1.7424, with an original deposit date of April 1, 2008. The *Sphingomonas hydrate* was purchased from the CGMCC, strain number CGMCC1.12070, with an original deposit date of January 10, 2012. The *Acinetobacter guangzhouensis* was purchased from the CGMCC, strain number CGMCC1.12967, with an original deposit date of July 14, 2014. All strains used in this invention can be purchased through the collection centers and do not require duplicate collection.
[0008] Furthermore, the preparation method of the compound microbial agent is as follows: Bacillus saccharophilus, Sphingospora hydrophila, and Acinetobacter guangzhouensis were activated separately, and single colonies were picked and inoculated into LB liquid medium and cultured at 30°C and 200 r / min until OD500. 600 =0.6 to obtain seed culture, and inoculate the seed culture evenly at a rate of 1% until the bacterial concentration reaches OD100. 600 When the concentration of the bacterial solution is 3.0, the three bacterial solutions are mixed evenly in a volume ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.
[0009] Furthermore, the preparation method of the functionalized biochar is as follows: Rice husks were used as raw material and biochar was produced by oxygen-limited pyrolysis at 500℃. The biochar was then mixed with a 0.5 mol / L diammonium hydrogen phosphate solution at a mass ratio of 1:10 and impregnated. Magnesium sulfate equivalent to 5% of the biochar mass was added, and the mixture was activated at 400℃ for 2 hours. After cooling, washing, and drying, functionalized biochar was obtained.
[0010] Furthermore, the mineral activator is potassium feldspar powder and phosphate rock powder in a mass ratio of 1:1, and the decomposed poultry and livestock manure is chicken manure.
[0011] A method for preparing bio-organic fertilizer with edible mushroom residue as the main material includes the following steps: (1) Dry the edible mushroom residue to reduce the moisture content to 45-50%, crush it to a particle size of less than 2cm, and evenly sprinkle one-third of the compound microbial agent into the edible mushroom residue. Pile it into strips for fermentation for 10-15 days until it is fully decomposed. (2) Spread out the fermented edible fungus residue, and add the decomposed poultry and livestock manure, humic acid, mineral activator, seaweed extract, functional biochar, polyglutamic acid and the remaining compound microbial agent in sequence, and mix evenly; (3) The mixed materials are aged for 5-7 days under ventilated and light-proof conditions, and then sent to a disc granulator for granulation. Particles with a diameter of 3-5 mm are screened out to obtain the final product, a biological organic fertilizer with edible mushroom residue as the main material.
[0012] This invention also provides the application of a bio-organic fertilizer with edible mushroom residue as the main material, which is used to improve the physical and chemical properties of soil and increase tomato yield and quality.
[0013] This invention screened three functional bacterial strains: *Bacillus saccharophilus*, *Sphingomonas hydrate*, and *Acinetobacter guangzhouensis*. *Bacillus saccharophilus* produces the plant hormone indoleacetic acid, which directly stimulates root development and plant growth, forming a larger and stronger root network. A well-developed root system means a stronger ability to absorb water and nutrients, resulting in more vigorous plant growth and improved stress resistance. *Sphingomonas hydrate* converts fixed phosphorus and potassium in the soil into available forms, improving soil fertility; it also improves soil physical structure, increases aeration, and helps other bacterial strains and roots better access nutrients. *Acinetobacter guangzhouensis* secretes organic acids to dissolve insoluble phosphates in the soil, releasing phosphorus that can be absorbed by plants; it also secretes siderophores to efficiently chelate iron ions in the soil for plant use. In addition, Bacillus saccharophilus, Sphingomyces hydrophila, and Acinetobacter guangzhouensis can all secrete cellulase, which can effectively degrade cellulose. When the three strains are combined, they have a synergistic effect, systematically and rapidly decompose large organic molecules into usable small molecules, resulting in good composting effect.
[0014] This invention incorporates functionalized biochar. The activation by phosphate significantly increases the specific surface area and pore structure of the biochar. This porous structure provides an ideal habitat for the composite microbial inoculant, promoting the colonization and reproduction of functional microorganisms. Furthermore, magnesium is loaded into the biochar pores in a stable form, allowing for slow release into the soil and effectively supplementing the magnesium required by crops.
[0015] Beneficial effects This invention produces a bio-organic fertilizer by screening functional microbial strains and combining them with other substances. On the one hand, the active substances produced by the metabolism of each strain effectively promote the decomposition of edible mushroom residue and dissolve elements such as phosphorus and potassium in the soil that are difficult for plants to absorb. At the same time, it forms a stable soil aggregate structure, making the soil looser, more breathable, and better able to retain water and fertilizer, reducing fertilizer loss and thus comprehensively improving soil fertility. On the other hand, it produces plant hormones to promote plant growth.
[0016] This invention's bio-organic fertilizer not only enables the resource utilization of edible mushroom residue and reduces environmental pollution, but also significantly improves soil physical and chemical properties, enhances soil fertility, and effectively increases tomato yield and quality. Attached Figure Description
[0017] Figure 1 The images show Congo red staining of the three strains of this invention; Note: a is Bacillus saccharophilus, b is Sphingospora hydrophila, and c is Acinetobacter guangzhouensis. Detailed Implementation
[0018] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.
[0019] Example 1 A bio-organic fertilizer with edible mushroom residue as the main material is characterized by comprising the following raw materials in parts by weight: 60 parts edible mushroom residue, 15 parts decomposed poultry and livestock manure, 5 parts humic acid, 3 parts compound microbial agent, 0.5 parts polyglutamic acid, 3 parts mineral activator, 2 parts seaweed extract, and 3 parts functionalized biochar.
[0020] The edible mushroom residue is oyster mushroom residue.
[0021] The compound microbial agent includes *Bacillus saccharophilus*, *Sphingospora hydrophila*, and *Acinetobacter guangzhouensis*; the strain number of *Bacillus saccharophilus* is CGMCC 1.7424; the strain number of *Sphingospora hydrophila* is CGMCC 1.12070; and the strain number of *Acinetobacter guangzhouensis* is CGMCC 1.12967.
[0022] The preparation method of the compound microbial agent is as follows: Bacillus saccharophilus, Sphingospora hydrophila, and Acinetobacter guangzhouensis were activated separately, and single colonies were picked and inoculated into LB liquid medium and cultured at 30°C and 200 r / min until OD500. 600 =0.6 to obtain seed culture, and inoculate the seed culture evenly at a rate of 1% until the bacterial concentration reaches OD100. 600 When the concentration of the bacterial solution is 3.0, the three bacterial solutions are mixed evenly in a volume ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.
[0023] The preparation method of the functionalized biochar is as follows: Rice husks were used as raw material and biochar was produced by oxygen-limited pyrolysis at 500℃. The biochar was then mixed with a 0.5 mol / L diammonium hydrogen phosphate solution at a mass ratio of 1:10 and impregnated. Magnesium sulfate equivalent to 5% of the biochar mass was added, and the mixture was activated at 400℃ for 2 hours. After cooling, washing, and drying, functionalized biochar was obtained.
[0024] The mineral activator is potassium feldspar powder and phosphate rock powder in a mass ratio of 1:1, and the decomposed poultry and livestock manure is chicken manure.
[0025] A method for preparing bio-organic fertilizer with edible mushroom residue as the main material includes the following steps: (1) Dry the edible mushroom residue to reduce the moisture content to 45%, crush it to a particle size of less than 2cm, and evenly sprinkle one-third of the compound microbial agent into the edible mushroom residue. Pile it into strips for fermentation for 10-15 days until it is fully decomposed. (2) Spread out the fermented edible fungus residue, and add the decomposed poultry and livestock manure, humic acid, mineral activator, seaweed extract, functional biochar, polyglutamic acid and the remaining compound microbial agent in sequence, and mix evenly; (3) The mixed materials are aged for 5 days under ventilated and light-proof conditions, and then sent to a disc granulator for granulation. Particles with a diameter of 3-5mm are screened out to obtain the final product, a biological organic fertilizer with edible mushroom residue as the main material.
[0026] Example 2 A bio-organic fertilizer with edible mushroom residue as the main ingredient is characterized by comprising the following raw materials in parts by weight: 68 parts edible mushroom residue, 20 parts decomposed poultry and livestock manure, 7 parts humic acid, 4 parts compound microbial agent, 1 part polyglutamic acid, 4 parts mineral activator, 3 parts seaweed extract, and 5 parts functionalized biochar.
[0027] The edible mushroom residue is oyster mushroom residue.
[0028] The compound microbial agent includes *Bacillus saccharophilus*, *Sphingospora hydrophila*, and *Acinetobacter guangzhouensis*; the strain number of *Bacillus saccharophilus* is CGMCC 1.7424; the strain number of *Sphingospora hydrophila* is CGMCC 1.12070; and the strain number of *Acinetobacter guangzhouensis* is CGMCC 1.12967.
[0029] The preparation method of the compound microbial agent is as follows: Bacillus saccharophilus, Sphingospora hydrophila, and Acinetobacter guangzhouensis were activated separately, and single colonies were picked and inoculated into LB liquid medium and cultured at 30°C and 200 r / min until OD500. 600 =0.6 to obtain seed culture, and inoculate the seed culture evenly at a rate of 1% until the bacterial concentration reaches OD100. 600 When the concentration of the bacterial solution is 3.0, the three bacterial solutions are mixed evenly in a volume ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.
[0030] The preparation method of the functionalized biochar is as follows: Rice husks were used as raw material and biochar was produced by oxygen-limited pyrolysis at 500℃. The biochar was then mixed with a 0.5 mol / L diammonium hydrogen phosphate solution at a mass ratio of 1:10 and impregnated. Magnesium sulfate equivalent to 5% of the biochar mass was added, and the mixture was activated at 400℃ for 2 hours. After cooling, washing, and drying, functionalized biochar was obtained.
[0031] The mineral activator is potassium feldspar powder and phosphate rock powder in a mass ratio of 1:1, and the decomposed poultry and livestock manure is chicken manure.
[0032] A method for preparing bio-organic fertilizer with edible mushroom residue as the main material includes the following steps: (1) Dry the edible mushroom residue until the moisture content drops to 50%, crush it to a particle size of less than 2cm, evenly sprinkle one-third of the compound microbial agent into the edible mushroom residue, pile it into strips for fermentation, continue for 10-15 days until it is fully decomposed; (2) Spread out the fermented edible fungus residue, and add the decomposed poultry and livestock manure, humic acid, mineral activator, seaweed extract, functional biochar, polyglutamic acid and the remaining compound microbial agent in sequence, and mix evenly; (3) The mixed materials are aged for 6 days under ventilated and light-proof conditions, and then sent to a disc granulator for granulation. Particles with a diameter of 3-5mm are screened out to obtain the final product, a biological organic fertilizer with edible mushroom residue as the main material.
[0033] Example 3 A bio-organic fertilizer with edible mushroom residue as the main ingredient is characterized by comprising the following raw materials in parts by weight: 75 parts edible mushroom residue, 25 parts decomposed poultry and livestock manure, 8 parts humic acid, 5 parts compound microbial agent, 1.5 parts polyglutamic acid, 5 parts mineral activator, 4 parts seaweed extract, and 6 parts functionalized biochar.
[0034] The edible mushroom residue is oyster mushroom residue.
[0035] The compound microbial agent includes *Bacillus saccharophilus*, *Sphingospora hydrophila*, and *Acinetobacter guangzhouensis*; the strain number of *Bacillus saccharophilus* is CGMCC1.7424; the strain number of *Sphingospora hydrophila* is CGMCC1.12070; and the strain number of *Acinetobacter guangzhouensis* is CGMCC1.12967.
[0036] The preparation method of the compound microbial agent is as follows: Bacillus saccharophilus, Sphingospora hydrophila, and Acinetobacter guangzhouensis were activated separately, and single colonies were picked and inoculated into LB liquid medium and cultured at 30°C and 200 r / min until OD500. 600 =0.6 to obtain seed culture, and inoculate the seed culture evenly at a rate of 1% until the bacterial concentration reaches OD100. 600 When the concentration of the bacterial solution is 3.0, the three bacterial solutions are mixed evenly in a volume ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.
[0037] The preparation method of the functionalized biochar is as follows: Rice husks were used as raw material and biochar was produced by oxygen-limited pyrolysis at 500℃. The biochar was then mixed with a 0.5 mol / L diammonium hydrogen phosphate solution at a mass ratio of 1:10 and impregnated. Magnesium sulfate equivalent to 5% of the biochar mass was added, and the mixture was activated at 400℃ for 2 hours. After cooling, washing, and drying, functionalized biochar was obtained.
[0038] The mineral activator is potassium feldspar powder and phosphate rock powder in a mass ratio of 1:1, and the decomposed poultry and livestock manure is chicken manure.
[0039] A method for preparing bio-organic fertilizer with edible mushroom residue as the main material includes the following steps: (1) Dry the edible mushroom residue until the moisture content drops to 50%, crush it to a particle size of less than 2cm, evenly sprinkle one-third of the compound microbial agent into the edible mushroom residue, pile it into strips for fermentation, continue for 10-15 days until it is fully decomposed; (2) Spread out the fermented edible fungus residue, and add the decomposed poultry and livestock manure, humic acid, mineral activator, seaweed extract, functional biochar, polyglutamic acid and the remaining compound microbial agent in sequence, and mix evenly; (3) The mixed materials are aged for 7 days under ventilated and light-proof conditions, and then sent to a disc granulator for granulation. Particles with a diameter of 3-5mm are screened out to obtain the final product, a biological organic fertilizer with edible mushroom residue as the main material.
[0040] Comparative Example 1 Compared with Example 3, this comparative example is identical to Example 3 except that the composite microorganisms are changed to Bacillus saccharophilus and Sphingosine monophosphate in a volume ratio of 1:1.
[0041] Comparative Example 2 Compared with Example 3, this comparative example is identical to Example 3 except that the composite microorganisms are changed to Bacillus saccharophilus and Acinetobacter guangzhou in a volume ratio of 1:1.
[0042] Comparative Example 3 Compared with Example 3, this comparative example is identical to Example 3 except that the composite microorganisms are changed to Sphingomyelin-Sphingosine monocytogenes and Acinetobacter guangzhou in a volume ratio of 1:1.
[0043] Comparative Example 4 Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent only uses Bacillus saccharophilus.
[0044] Comparative Example 5 Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent only uses Sphingosine hydrophila.
[0045] Comparative Example 6 Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent only uses Acinetobacter guangnanensis.
[0046] Comparative Example 7 Compared with Example 3, this comparative example is identical to Example 3 except that no modified biochar is added. All other raw materials and steps are the same as in Example 3.
[0047] Performance testing Cellulase assay: First, culture each bacterial strain on a plate and measure the colony diameter. Then, spread 1% Congo red solution onto the plate and let it stand for 15-30 minutes. After staining, add 1 mol / L sodium chloride solution to stop the reaction. After 15 minutes, discard the NaCl solution. During this process, at the colonies that produce cellulase, such as... Figure 1 (a is Bacillus saccharophilus, b is Sphingosporobacter hydrate, c is Acinetobacter guangzhouensis) As shown, cellulase production will produce a distinct clear zone.
[0048] Depend on Figure 1 The saccharophilic Bacillus, Sphingosine hydrophila, and Acinetobacter guangzhouensis shown can all secrete cellulase.
[0049] Planting Trial The tomato variety tested was "Xiangnai". The soil physicochemical properties of the planting site were as follows: total porosity 46.5%, organic matter content 26.67 g / kg, available nitrogen 110.57 mg / kg, available phosphorus content 24.92 mg / kg, and available potassium content 201.85 mg / kg.
[0050] The experiment consisted of 11 experimental groups: one using the organic fertilizers described in Examples 1-3 and Comparative Examples 1-7, and a blank control (no fertilizer). The fertilizer application rate for each treatment group was 1500 kg / mu (approximately 1000 kg / acre). Each treatment group was replicated three times. Each plot was 5 × 5 m in a randomized block design. Other management practices remained consistent with routine practices. After fruit maturity, plot yields were measured and converted to per-acre yields. Ten tomatoes were randomly selected from each treatment group to determine soluble sugars, soluble proteins, vitamin C, organic acids, and lycopene. Soluble sugars were measured using the anthrone colorimetric method; soluble proteins were measured using Coomassie Brilliant Blue G-250 staining; vitamin C was determined using the 2,6-dichlorophenolindophenol titration method; organic acids were determined using acid-base titration; and lycopene was determined using a UV spectrophotometer. The data are shown in Table 1.
[0051] Table 1. Tomato yield and quality of each treatment group The data above shows that using the bio-organic fertilizer of Examples 1-3 of this invention significantly increases tomato yield and further improves tomato quality, mainly manifested in a significant increase in microbial content, lycopene content, and soluble protein content, as well as an increase in soluble sugar content. In contrast, Comparative Examples 1-7, which altered the composition of the organic fertilizer of this invention, showed varying degrees of decrease in tomato yield and quality. Firstly, the significantly reduced effect of the organic fertilizer in Comparative Examples 1-6 indicates that the synergistic effect of the three strains used in this invention was disrupted. Secondly, the lack of modified biochar also reduced fertilizer efficiency. This demonstrates that the *Bacillus saccharophilus*, *Sphingomyelin-hydatid*, *Acinetobacter guangzhouensis*, and modified biochar selected in this invention are all synergistic components; the absence of any one of them weakens the effect.
[0052] Soil sample collection and testing After tomato harvest, soil samples from the 0-20cm soil layer in each treatment group were collected using a 5-point sampling method. Soil porosity was determined using the ring cutter method; organic matter content was determined using the potassium dichromate-external heating oxidation method; alkaline nitrogen content was determined using the alkaline diffusion method; available phosphorus content was determined using the 0.5 mol / L NaHCO3 extraction-molybdenum antimony spectrophotometric method; and available potassium content was determined using the 1 mol / L CH3COONH4 extraction-flame atomic absorption spectrometry. The above test data are shown in Table 2.
[0053] Table 2 Soil physicochemical properties The data above show that the bio-organic fertilizer of this invention can effectively improve soil porosity and significantly enhance soil fertility, specifically manifested in a significant increase in the content of organic matter, available nitrogen, available phosphorus, and available potassium. Compared with the original soil, the contents of available nitrogen, available phosphorus, and available potassium were significantly increased in Examples 1-3, with Example 3 showing the largest increase, indicating that the bio-organic fertilizer of this invention helps to increase the content of nitrogen, phosphorus, and potassium nutrients in the soil. Compared with the original soil, the soil organic matter content also increased after using the bio-organic fertilizer of this invention, with Example 3 showing the best effect, increasing the organic matter content by 26.4% compared with the original soil.
[0054] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.
Claims
1. A bio-organic fertilizer mainly composed of edible mushroom residue, characterized in that, The raw materials include the following parts by weight: 60-75 parts edible mushroom residue, 15-25 parts decomposed poultry and livestock manure, 5-8 parts humic acid, 3-5 parts compound microbial agent, 0.5-1.5 parts polyglutamic acid, 3-5 parts mineral activator, 2-4 parts seaweed extract, and 3-6 parts functionalized biochar.
2. The bio-organic fertilizer based on edible mushroom residue as the main material according to claim 1, characterized in that, The edible mushroom residue is oyster mushroom residue.
3. The bio-organic fertilizer based on edible mushroom residue as the main material according to claim 1, characterized in that, The compound microbial agent includes *Bacillus saccharophilus*, *Sphingospora hydrophila*, and *Acinetobacter guangzhouensis*; the strain number of *Bacillus saccharophilus* is CGMCC 1.7424, the strain number of *Sphingospora hydrophila* is CGMCC 1.12070, and the strain number of *Acinetobacter guangzhouensis* is CGMCC 1.12967.
4. The bio-organic fertilizer based on edible mushroom residue as the main material according to claim 1, characterized in that, The preparation method of the compound microbial agent is as follows: Bacillus saccharophilus, Sphingospora hydrophila, and Acinetobacter guangzhouensis were activated separately, and single colonies were picked and inoculated into LB liquid medium and cultured at 30°C and 200 r / min until OD500. 600 =0.6 to obtain seed culture, and inoculate the seed culture evenly at a rate of 1% until the bacterial concentration reaches OD100. 600 When the concentration of the bacterial solution is 3.0, the three bacterial solutions are mixed evenly in a volume ratio of 1:1:1 and freeze-dried into freeze-dried powder to obtain a compound microbial agent.
5. The bio-organic fertilizer based on edible mushroom residue as the main material according to claim 1, characterized in that, The preparation method of the functionalized biochar is as follows: Rice husks were used as raw material and biochar was produced by oxygen-limited pyrolysis at 500℃. The biochar was then mixed with a 0.5 mol / L diammonium hydrogen phosphate solution at a mass ratio of 1:10 and impregnated. Magnesium sulfate equivalent to 5% of the biochar mass was added, and the mixture was activated at 400℃ for 2 hours. After cooling, washing, and drying, functionalized biochar was obtained.
6. The bio-organic fertilizer based on edible mushroom residue as the main material according to claim 1, characterized in that, The mineral activator is potassium feldspar powder and phosphate rock powder in a mass ratio of 1:1, and the decomposed poultry and livestock manure is chicken manure.
7. A method for preparing a bio-organic fertilizer based primarily on edible mushroom residue as described in any one of claims 1-6, characterized in that, Includes the following steps: (1) Dry the edible mushroom residue to reduce the moisture content to 45-50%, crush it to a particle size of less than 2cm, and evenly sprinkle one-third of the compound microbial agent into the edible mushroom residue. Pile it into strips for fermentation for 10-15 days until it is fully decomposed. (2) Spread out the fermented edible fungus residue, and add the decomposed poultry and livestock manure, humic acid, mineral activator, seaweed extract, functional biochar, polyglutamic acid and the remaining compound microbial agent in sequence, and mix evenly; (3) The mixed materials are aged for 5-7 days under ventilated and light-proof conditions, and then sent to a disc granulator for granulation. Particles with a diameter of 3-5 mm are screened out to obtain the final product, a biological organic fertilizer with edible mushroom residue as the main material.
8. The application of the bio-organic fertilizer based on edible mushroom residue as described in claim 1, characterized in that, The bio-organic fertilizer is used to improve the physical and chemical properties of the soil and increase tomato yield and quality.