Preparation method of probiotic down feather

Abstract

The invention belongs to the technical field of down feather processing, and discloses a preparation method of probiotic down feather, which comprises the following steps: carrying out impurity removal, degreasing and cleaning operation on down feather; adding the clean down feather into the pretreatment liquid, and sealing, stirring and soaking; adding acid to carry out gradual acid reduction treatment, then adding an acidic enzyme preparation, stirring and soaking; then adding acid to carry out acid reduction treatment to obtain softened and hydrophilic down feather; adding high-concentration probiotic treatment liquid for soaking, removing the liquid, continuously drying the down feather subjected to probiotic treatment by adopting circulating cold air, introducing the down feather into low-concentration probiotic treatment liquid by the circulating cold air every 30 minutes for wetting treatment, washing the down feather with deionized water, completely drying the down feather by the circulating cold air, and bagging the down feather for later use; the down feather is subjected to acid treatment softening and affinity through pretreatment, so that the down feather better and more uniformly adsorbs and adheres to a probiotic layer, and active ingredients in probiotics are used for preventing mites and bacteria and maintaining bacterial colony balance; and meanwhile, the probiotic down feather keeps good softness and comfort.

Description

Technical Field

[0001] This invention relates to the field of down processing technology, and specifically to a method for preparing probiotic down. Background Technology

[0002] Down is a lightweight, warm material widely used in down jackets and down comforters. A down cluster is a cluster-like structure composed of a core and numerous radiating filaments. Each filament is made up of thousands of tiny, hollow scales, and each filament has many interlocking segments that trap still air. Simultaneously, a large amount of air is also trapped between the filaments and the cluster, giving down its strong insulating properties. Before processing, down typically undergoes washing and treatment, followed by drying.

[0003] Existing apparel products primarily utilize probiotics in nonwoven fabrics. For example, application number 202410106483.7 describes a probiotic nonwoven fabric and its preparation method and application. This method reduces the requirements for pH, high temperature, and other factors during the production of probiotic nonwoven fabrics by using probiotic microcapsules. Simultaneously, it allows the probiotic microcapsules to be stably adhered to the nonwoven fabric without the use of adhesives, resulting in better application quality. The applicant's research has discovered that probiotics can be applied to down to enhance its antibacterial and other properties. Summary of the Invention

[0004] In view of the above-mentioned defects or improvement needs of the existing technology, the present invention provides a method for preparing probiotic down.

[0005] To achieve the above objectives, according to one aspect of the present invention, a method for preparing probiotic down is provided, comprising the following steps: S1: Perform impurity removal, degreasing, and washing operations on the down feathers; S2: Add clean down to the pretreatment solution, seal and stir for 2-5 hours; add acid to gradually reduce the pH value, each time the pH value decreases by 0.5, stir and soak for 20-30 minutes, then reduce the pH value again until the pH value is controlled at 4.0-4.5; then add acidic enzyme preparation, stir and soak for 30-60 minutes; add acid again to reduce the pH value until the pH value is controlled at 3.0-4.0, stir for 20-30 minutes, and soak for 5-10 minutes. Repeat the intermittent treatment at least 5-10 times to obtain softened and compatible down. S3: After drying the treated down with circulating air to a humidity of 30-40%, soak it in a high-concentration probiotic treatment solution for 60-300 minutes, remove the liquid, and continue to dry the probiotic-treated down with circulating cold air. Every 30 minutes, a low-concentration probiotic treatment solution is introduced by circulating cold air for wetting treatment. Repeat at least 3-6 times. S4: After the probiotic treatment, rinse the down feathers 2-3 times with deionized water, then dry them completely with circulating cold air, pack them in bags, and set aside for later use.

[0006] Preferably, the pretreatment solution is 1-allyl-3-methylimidazolium chloride, and the acid is one or a combination of formic acid and acetic acid.

[0007] Preferably, during the pretreatment process, the volume ratio of down to pretreatment liquid is 1:1.2~2.

[0008] Preferably, the high-concentration probiotic treatment solution comprises, by weight, 10-30 parts of inactivated probiotic powder, 3-6 parts of acidic enzyme preparation, 1-5 parts of wood pulp fiber, 0.5-2 parts of sodium alginate, 0.5-2 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.1-0.5 parts of butylated hydroxytoluene, 0.1-0.5 parts of polylysine, and 100 parts of deionized water.

[0009] Preferably, the volume ratio of the down to the high-concentration probiotic treatment solution is 1:1~2.

[0010] Preferably, the low-concentration probiotic treatment solution comprises, by weight, 1-5 parts of inactivated probiotic powder, 3-6 parts of acidic enzyme preparation, 1-5 parts of wood pulp fiber, 0.5-2 parts of sodium alginate, 0.5-2 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.1-0.5 parts of butylated hydroxytoluene, 0.1-0.5 parts of polylysine, and 100 parts of deionized water.

[0011] Preferably, the inactivated probiotic powder is one or a combination of Bacillus thermophilus, Bacillus subtilis, Bacillus licheniformis, Lactobacillus bulgaricus, Lactobacillus acidophilus, and Bifidobacterium lactis.

[0012] Preferably, the acidic enzyme preparation is AB protease.

[0013] Preferably, the temperature of the circulating air is 30-50℃, and the temperature of the circulating cold air is not higher than 35℃.

[0014] In summary, the technical solutions conceived by this invention have the following beneficial effects compared with the prior art: The probiotic down preparation method of the present invention softens and acclimates the down by acid treatment in the pretreatment, so that the down can better and more evenly adsorb and adhere the probiotic layer. The active ingredients in the probiotics can prevent mites, kill bacteria and maintain the balance of the colony. At the same time, the probiotic down maintains good softness and comfort. Detailed Implementation

[0015] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. Furthermore, the technical features involved in the various embodiments of this invention described below can be combined with each other as long as they do not conflict with each other.

[0016] Example 1 A method for preparing probiotic down includes the following steps: S1: Perform impurity removal, degreasing, and washing operations on the down feathers; S2: Add clean down to the pretreatment solution, seal and stir for 2 hours; add acid for gradual pH reduction treatment, each time the pH value decreases by 0.5, stir and soak for 20-30 minutes, then repeat the pH reduction treatment until the pH value is controlled at 4.0-4.5; then add acidic enzyme preparation, stir and soak for 30-60 minutes; add acid again for pH reduction treatment until the pH value is controlled at 3.0-4.0, stir for 20-30 minutes, and soak for 5-10 minutes. Repeat the intermittent treatment at least 5 times to obtain softened and compatible down; the pretreatment solution is 1-allyl-3-methylimidazolium chloride, the volume ratio of down to pretreatment solution is 1:1.2, and the acid is formic acid; S3: When the treated down is dried to a humidity of 30-40% using circulating air and the circulating air temperature is 30-50℃, add high-concentration probiotic treatment solution and soak for 60-300 minutes. Remove the liquid and continue to dry the probiotic-treated down using circulating cold air. Every 30 minutes, low-concentration probiotic treatment solution is introduced by circulating cold air for wetting treatment. Repeat at least 3 times. S4: After the probiotic treatment, rinse the down feathers twice with deionized water and then dry them completely with circulating cold air at a temperature not exceeding 35°C. Pack them in bags for later use.

[0017] The high-concentration probiotic treatment solution comprises, by weight, 10 parts of inactivated probiotic powder, 3 parts of acidic enzyme preparation, 2 parts of wood pulp fiber, 0.8 parts of sodium alginate, 0.8 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.2 parts of butylated hydroxytoluene, 0.2 parts of polylysine, and 100 parts of deionized water. The volume ratio of down to the high-concentration probiotic treatment solution is 1:1.

[0018] The low-concentration probiotic treatment solution comprises, by weight, 2 parts inactivated probiotic powder, 3 parts acidic enzyme preparation, 2 parts wood pulp fiber, 0.8 parts sodium alginate, 0.8 parts polymethyl methacrylate-β-hydroxyethyl ester, 0.2 parts butylated hydroxytoluene, 0.2 parts polylysine, and 100 parts deionized water.

[0019] The inactivated probiotic powder is Bacillus thermophilus; the acidic enzyme preparation is AB protease.

[0020] Example 2 A method for preparing probiotic down includes the following steps: S1: Perform impurity removal, degreasing, and washing operations on the down feathers; S2: Add clean down to the pretreatment solution, seal and stir for 3 hours; add acid for gradual pH reduction treatment, each time the pH value decreases by 0.5, stir and soak for 20-30 minutes, then repeat the pH reduction treatment until the pH value is controlled at 4.0-4.5; then add acidic enzyme preparation, stir and soak for 30-60 minutes; add acid again for pH reduction treatment until the pH value is controlled at 3.0-4.0, stir for 20-30 minutes, then soak for 5-10 minutes, repeat the intermittent treatment at least 6 times to obtain softened and compatible down; the pretreatment solution is 1-allyl-3-methylimidazolium chloride, the volume ratio of down to pretreatment solution is 1:1.5, and the acid is acetic acid; S3: When the treated down is dried to a humidity of 30-40% using circulating air and the circulating air temperature is 30-50℃, add high-concentration probiotic treatment solution and soak for 60-300 minutes. Remove the liquid and continue to dry the probiotic-treated down using circulating cold air. Every 30 minutes, low-concentration probiotic treatment solution is introduced by circulating cold air for wetting treatment. Repeat at least 5 times. S4: After the probiotic treatment, rinse the down feathers three times with deionized water and then dry them completely with circulating cold air at a temperature not exceeding 35°C. Pack them in bags for later use.

[0021] The high-concentration probiotic treatment solution comprises, by weight, 20 parts of inactivated probiotic powder, 5 parts of acidic enzyme preparation, 3 parts of wood pulp fiber, 1 part of sodium alginate, 0.8 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.5 parts of butylated hydroxytoluene, 0.3 parts of polylysine, and 100 parts of deionized water. The volume ratio of down to the high-concentration probiotic treatment solution is 1:1.5.

[0022] The low-concentration probiotic treatment solution comprises, by weight, 1 part inactivated probiotic powder, 5 parts acidic enzyme preparation, 3 parts wood pulp fiber, 1 part sodium alginate, 0.8 parts polymethyl methacrylate-β-hydroxyethyl ester, 0.5 parts butylated hydroxytoluene, 0.3 parts polylysine, and 100 parts deionized water.

[0023] The inactivated probiotic powder is composed of Bacillus thermophilus and Bacillus subtilis; the acidic enzyme preparation is AB protease.

[0024] Example 3 A method for preparing probiotic down includes the following steps: S1: Perform impurity removal, degreasing, and washing operations on the down feathers; S2: Add clean down to the pretreatment solution, seal and stir for 5 hours; add acid for gradual pH reduction treatment, each time the pH value decreases by 0.5, stir and soak for 20-30 minutes, then repeat the pH reduction treatment until the pH value is controlled at 4.0-4.5; then add acidic enzyme preparation, stir and soak for 30-60 minutes; add acid again for pH reduction treatment until the pH value is controlled at 3.0-4.0, stir for 20-30 minutes, then soak for 5-10 minutes, repeat the intermittent treatment at least 10 times to obtain softened and compatible down; the pretreatment solution is 1-allyl-3-methylimidazolium chloride, the volume ratio of down to pretreatment solution is 1:2, and the acid is acetic acid; S3: When the treated down is dried to a humidity of 30-40% using circulating air and the circulating air temperature is 30-50℃, add high-concentration probiotic treatment solution and soak for 60-300 minutes. Remove the liquid and continue to dry the probiotic-treated down using circulating cold air. Every 30 minutes, low-concentration probiotic treatment solution is introduced by circulating cold air for wetting treatment. Repeat at least 6 times. S4: After the probiotic treatment, rinse the down feathers three times with deionized water and then dry them completely with circulating cold air at a temperature not exceeding 35°C. Pack them in bags for later use.

[0025] The high-concentration probiotic treatment solution comprises, by weight, 30 parts of inactivated probiotic powder, 6 parts of acidic enzyme preparation, 5 parts of wood pulp fiber, 2 parts of sodium alginate, 2 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.5 parts of butylated hydroxytoluene, 0.5 parts of polylysine, and 100 parts of deionized water. The volume ratio of down to the high-concentration probiotic treatment solution is 1:2.

[0026] The low-concentration probiotic treatment solution comprises, by weight, 5 parts of inactivated probiotic powder, 6 parts of acidic enzyme preparation, 5 parts of wood pulp fiber, 2 parts of sodium alginate, 2 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.5 parts of butylated hydroxytoluene, 0.5 parts of polylysine, and 100 parts of deionized water.

[0027] The inactivated probiotic powder is Bacillus licheniformis; the acidic enzyme preparation is AB protease.

[0028] The probiotic down prepared in Examples 1-3 were tested for its anti-mite performance and antibacterial effect.

[0029] (1) Anti-mite performance The inhibition method according to GB / T 24253-2009 9.2 was adopted. The dust mite was selected for testing. The culture period was 7 days. The sample pretreatment was drying at 65℃ for 10 min. The surviving adult mites and nymphs in the culture dish were observed and recorded using a dissecting microscope. The evaluation criteria were: inhibition rate ≥95%, the sample has a very strong mite-proof effect; inhibition rate ≥80%, the sample has a relatively strong mite-proof effect; inhibition rate ≥60%, the sample has a mite-proof effect. The down used for comparison was down that was only cleaned in step S1. The specific test results are shown in Table 1.

[0030] Table 1 Serial Number Inhibition rate Effect evaluation Comparison of down 50.81% The sample did not have an anti-mite effect. Example 1 70.83% The sample has anti-mite effect Example 2 70.62% The sample has anti-mite effect Example 3 70.50% The sample has anti-mite effect The above experiments show that this probiotic down has an anti-mite effect.

[0031] (2) Antibacterial effect [Klebsiella pneumoniae] The antibacterial efficacy of Klebsiella pneumoniae was tested using the shaking method described in GB / T 20944.3-2008. The samples were sterilized by autoclaving, and the contact time between the samples and the bacterial suspension was 18 hours. The control sample was prepared using 100% cotton fabric. The bacterial concentration (CFU / mL) for ATCC 4352 Klebsiella pneumoniae was 3.3 x 10⁻⁶. 5 The standard requirement for antibacterial rate is ≥70%, and the specific test results are shown in Table 2.

[0032] Table 2 Serial Number Total number of colonies within 0h contact time Total number of colonies after incubation Antibacterial rate Effect evaluation 100% cotton fabric 2.3*10 4 ]]> 8.7*10 6 ]]> / No antibacterial effect Example 1 2.3*10 4 ]]> 6.3*10 4 ]]> 99% It has antibacterial effects Example 2 2.3*10 4 ]]> 6.4*10 4 ]]> 99% It has antibacterial effects Example 3 2.3*10 4 ]]> 6.0*10 4 ]]> 99% It has antibacterial effects The above experiments show that this probiotic down has an antibacterial effect against Klebsiella pneumoniae.

[0033] (3) Antibacterial effect [Staphylococcus aureus] The antibacterial efficacy against Staphylococcus aureus was tested using the shaking method described in GB / T 20944.3-2008. The samples were sterilized by autoclaving, and the contact time between the samples and the bacterial suspension was 18 hours. The control sample was prepared using 100% cotton fabric. The bacterial concentration (CFU / mL) for ATCC 6538 Staphylococcus aureus was 3.2 × 10⁻⁶. 5 The standard requirement for antibacterial rate is ≥70%, and the specific test results are shown in Table 3.

[0034] Table 3 Serial Number Total number of colonies within 0h contact time Total number of colonies after incubation Antibacterial rate Effect evaluation 100% cotton fabric 2.1*10 4 ]]> 3.0*10 6 ]]> / No antibacterial effect Example 1 2.1*10 4 ]]> 2.0*10 2 ]]> ＞99% It has antibacterial effects Example 2 2.1*10 4 ]]> 2.6*10 2 ]]> ＞99% It has antibacterial effects Example 3 2.1*10 4 ]]> 2.2*10 2 ]]> ＞99% It has antibacterial effects The above experiments show that this probiotic down has an antibacterial effect against Staphylococcus aureus.

[0035] (4) Antibacterial effect [Escherichia coli] The antibacterial efficacy of *Escherichia coli* was tested using the shaking method according to GB / T 20944.3-2008. The samples were sterilized by autoclaving, and the contact time between the samples and the bacterial suspension was 18 hours. The control sample was made of 100% cotton fabric. The bacterial concentration (CFU / mL) was 8099 / 10⁹ / L and 3.4*10⁹ / L for *E. coli*. 5 The standard requirement for antibacterial rate is ≥70%, and the specific test results are shown in Table 4.

[0036] Table 4 Serial Number Total number of colonies within 0h contact time Total number of colonies after incubation Antibacterial rate Effect evaluation 100% cotton fabric 2.4*10 4 ]]> <![CDATA[8.2*10 6 ]]> / No antibacterial effect Example 1 <![CDATA[2.4*10 4 ]]> <![CDATA[5.4*10 5 ]]> 93.4% It has antibacterial effects Example 2 <![CDATA[2.4*10 4 ]]> <![CDATA[5.5*10 5 ]]> 93.3% It has antibacterial effects Example 3 <![CDATA[2.4*10 4 ]]> <![CDATA[5.1*10 5 ]]> 93.7% It has antibacterial effects The above experiments show that this probiotic down has an antibacterial effect against Escherichia coli.

[0037] (5) Antibacterial effect [Candida albicans] The antibacterial efficacy of Candida albicans was tested using the shaking method described in GB / T 20944.3-2008. The samples were sterilized by autoclaving, and the contact incubation time between the samples and the bacterial suspension was 18 hours. The control sample was prepared using 100% cotton fabric. The bacterial concentration (CFU / mL) for Candida albicans (ATCC 10231) was 3.0 × 10⁻⁶. 5 The standard requirement for antibacterial rate is ≥60%, and the specific test results are shown in Table 5.

[0038] Table 5 Serial Number Total number of colonies within 0h contact time Total number of colonies after incubation Antibacterial rate Effect evaluation 100% cotton fabric <![CDATA[2.0*10 4 ]]> <![CDATA[6.6*10 5 ]]> / No antibacterial effect Example 1 <![CDATA[2.0*10 4 ]]> <![CDATA[5.2*10 4 ]]> 92.1% It has antibacterial effects Example 2 <![CDATA[2.0*10 4 ]]> <![CDATA[5.3*10 4 ]]> 92.0% It has antibacterial effects Example 3 <![CDATA[2.0*10 4 ]]> <![CDATA[5.0*10 4 ]]> 92.4% It has antibacterial effects The above experiments show that this probiotic down has an antibacterial effect against Candida albicans.

[0039] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for preparing probiotic down, characterized in that, Includes the following steps: S1: Perform impurity removal, degreasing, and washing operations on the down feathers; S2: Add clean down to the pretreatment solution, seal and stir for 2-5 hours; add acid to gradually reduce the pH value, each time the pH value decreases by 0.5, stir and soak for 20-30 minutes, then reduce the pH value again until the pH value is controlled at 4.0-4.5; then add acidic enzyme preparation, stir and soak for 30-60 minutes; add acid again to reduce the pH value until the pH value is controlled at 3.0-4.0, stir for 20-30 minutes, and soak for 5-10 minutes. Repeat the intermittent treatment at least 5-10 times to obtain softened and compatible down. S3: After drying the treated down with circulating air to a humidity of 30-40%, soak it in a high-concentration probiotic treatment solution for 60-300 minutes, remove the liquid, and continue to dry the probiotic-treated down with circulating cold air. Every 30 minutes, a low-concentration probiotic treatment solution is introduced by circulating cold air for wetting treatment. Repeat at least 3-6 times. S4: After the probiotic treatment, rinse the down feathers 2-3 times with deionized water, then dry them completely with circulating cold air, pack them in bags, and set aside for later use.

2. The method for preparing probiotic down according to claim 1, characterized in that, The pretreatment solution is 1-allyl-3-methylimidazolium chloride, and the acid is one or a combination of formic acid and acetic acid.

3. The method for preparing probiotic down according to claim 2, characterized in that, During the pretreatment process, the volume ratio of down to pretreatment liquid is 1:1.2~2.

4. The method for preparing probiotic down according to claim 1, characterized in that, The high-concentration probiotic treatment solution comprises, by weight, 10-30 parts of inactivated probiotic powder, 3-6 parts of acidic enzyme preparation, 1-5 parts of wood pulp fiber, 0.5-2 parts of sodium alginate, 0.5-2 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.1-0.5 parts of butylated hydroxytoluene, 0.1-0.5 parts of polylysine, and 100 parts of deionized water.

5. The method for preparing probiotic down according to claim 4, characterized in that, The volume ratio of the down to the high-concentration probiotic treatment solution is 1:1~2.

6. The method for preparing probiotic down according to claim 1, characterized in that, The low-concentration probiotic treatment solution comprises, by weight, 1-5 parts of inactivated probiotic powder, 3-6 parts of acidic enzyme preparation, 1-5 parts of wood pulp fiber, 0.5-2 parts of sodium alginate, 0.5-2 parts of polymethyl methacrylate-β-hydroxyethyl ester, 0.1-0.5 parts of butylated hydroxytoluene, 0.1-0.5 parts of polylysine, and 100 parts of deionized water.

7. A method for preparing probiotic down according to claim 4, 5, or 6, characterized in that, The inactivated probiotic powder is one or a combination of several of the following: Bacillus thermophilus, Bacillus subtilis, Bacillus licheniformis, Lactobacillus bulgaricus, Lactobacillus acidophilus, and Bifidobacterium lactis.

8. A method for preparing probiotic down according to claim 4, 5, or 6, characterized in that, The acidic enzyme preparation is AB protease.

9. The method for preparing probiotic down according to claim 1, characterized in that, The temperature of the circulating air is 30-50℃, and the temperature of the circulating cold air is not higher than 35℃.