Gene sequencing library preparation equipment

Abstract

The invention provides gene sequencing library preparation equipment, which comprises an equipment main body frame, a pipetting device and a card box driving device are arranged on the equipment main body frame, the pipetting device is located at the top area of the card box driving device, and the card box driving device is used for bearing a layered card box and driving a target layer of the layered card box to rotate. The pipetting device comprises a first pipetting module and a second pipetting module for pipetting between the liquid storage tubes in the layered cartridge through the first pipetting module and the second pipetting module. The card box driving device can drive the target layer of the layered card box to rotate, so that the layered card box can be matched with the first pipetting module and the second pipetting module arranged on the pipetting device above the layered card box to realize pipetting operation among liquid storage pipes in the layered card box, and full-process operation of gene sequencing library preparation can be completed; and the structural compactness of the equipment is improved, and adverse phenomena such as cross contamination in the operation process can be avoided.

Description

Technical Field

[0001] This invention belongs to the field of biological detection technology, specifically relating to a gene sequencing library preparation device. Background Technology

[0002] Library preparation for gene sequencing is a well-established experimental procedure in NGS (noninvasive genetic sampling) sample pretreatment. Its purpose is to ligate specific linkers to both sides of the gene fragment to be sequenced, enabling subsequent fragment identification during sequencing to pinpoint the start and end bases of the target fragment, thus achieving the sequencing objective. Library preparation technology has significant applications in clinical disease diagnosis, animal disease detection, drug development, food safety, and scientific research.

[0003] IVD medical consumables refer to medical and sanitary materials used in clinical diagnosis, nursing, testing, and repair processes. They come in a wide variety of types and models and are widely used, serving as essential materials for routine medical and nursing work in medical institutions. Consumables, used in conjunction with machines, constitute part of the testing process. The driving and use of consumables are diverse, generally employing different driving methods when used with machines. In existing technologies, insertion and push-in methods are predominantly used for driving consumables, and consumables matching these methods are often multi-well rectangular matrix structures (such as 96-well plates). Due to their flat matrix structure, these consumables are large in size, occupying significant space, which is detrimental to the miniaturization of corresponding equipment. To overcome these shortcomings, a cartridge with a layered design along its height has been proposed in related technologies. At least one layer of the cartridge can be driven to rotate around the central axis of the cartridge, thus allowing the corresponding liquid reservoirs in different layers to be adjusted circumferentially to match the corresponding pipettes. Therefore, a gene sequencing library preparation device capable of driving the rotation of the corresponding layers within the cartridge is required. Summary of the Invention

[0004] Therefore, the technical problem to be solved by the present invention is to provide a gene sequencing library preparation device that can overcome the technical deficiency of the prior art in that there is no corresponding gene sequencing library preparation device for stratified cartridges.

[0005] To address the aforementioned problems, this invention provides a gene sequencing library preparation device, comprising a main frame, on which a pipetting device and a cartridge driving device are provided. The pipetting device is located at the top of the cartridge driving device, wherein the cartridge driving device is used to carry layered cartridges and drive the target layer of the layered cartridges to rotate. The pipetting device includes a first pipetting module and a second pipetting module for pipetting liquid between the storage tubes within the layered cartridges via the first and second pipetting modules.

[0006] In some embodiments, the pipetting device further includes a fixed frame, a lifting frame, and a rotating frame. The first pipetting module and the second pipetting module are assembled on the rotating frame. The pipetting device also includes a frame lifting drive assembly assembled on the fixed frame and a rotating drive assembly assembled on the lifting frame. The frame lifting drive assembly is used to drive the lifting frame to move up and down relative to the fixed frame in a vertical direction. The rotating frame is assembled on the lifting frame and can rotate around a first vertical axis under the drive of the rotating drive assembly to change the circumferential position of the first pipetting module and the second pipetting module.

[0007] In some embodiments, the first pipetting module includes a first pipette with its aspiration axis on a first cylindrical surface centered on the first vertical axis. The second pipetting module includes a second pipette with its aspiration axis on a second cylindrical surface centered on the first vertical axis, and the first cylindrical surface is radially outside the second cylindrical surface. The rotating frame includes a top turntable and a bottom turntable, both of which are rotatably supported on the lifting frame. The first pipetting module further includes a first lead screw motor and a first lifting block that can be driven to rise and fall by its lead screw, with the first pipette mounted on the first lifting block. The second pipetting module further includes a second lead screw motor and a second lifting block that can be driven to rise and fall by its lead screw, with the second pipette mounted on the second lifting block.

[0008] In some embodiments, a first guide connecting post and a second guide connecting post are provided between the top turntable and the bottom turntable, the first lifting block is slidably connected to the first guide connecting post, and the second lifting block is slidably connected to the second guide connecting post; and / or, the lifting frame includes a vertical plate, an upper horizontal plate at the upper end of the vertical plate, and a lower horizontal plate at the lower end of the vertical plate, the upper horizontal plate and the lower horizontal plate respectively have an upper rotating hole and a lower rotating hole coaxially, the top turntable is rotatably connected to the upper rotating hole, and the bottom turntable is rotatably connected to the lower rotating hole; and / or, a plurality of positioning plugs are provided on the bottom surface of the bottom turntable, each positioning plug being evenly spaced along the circumferential direction of the first vertical axis, and the distribution circle is the first cylindrical surface.

[0009] In some embodiments, a pipetting pump is provided on the top surface of the bottom turntable, and the pipetting pump is controllably connected to the first pipetting tube to enable the first pipetting tube to perform pipetting using a vacuum suction method; and / or, the second pipetting tube performs pipetting using a piston suction method, and the second pipetting tube also has a magnetic sleeve that can be controlled to extend and retract.

[0010] In some embodiments, the cartridge drive device includes a device frame, the device frame includes a top plate, the top plate has an assembly through hole, the assembly through hole has a cartridge connecting ring, the cartridge connecting ring is assembled in the assembly through hole by a bearing, the inner ring wall of the cartridge connecting ring has a locking groove, the device frame also has a rotation drive assembly, the rotation drive assembly is used to drive the cartridge connecting ring to rotate relative to the top plate.

[0011] In some embodiments, the device frame is further provided with a first temperature control module, which includes a heating unit and a refrigeration unit, and the heating unit and the refrigeration unit are arranged circumferentially around the rotation axis of the card box connecting ring.

[0012] In some embodiments, the device frame is further provided with a second temperature control module located in the area between the heating unit and the refrigeration unit. The second temperature control module includes a heat-conducting plate that extends along the diameter direction of the bearing. An electric heating film is provided on the bottom surface of the heat-conducting plate, and a plurality of second receiving grooves are provided on the top surface of the heat-conducting plate.

[0013] In some embodiments, the device frame is further provided with a magnetic attraction module, which includes a magnetic attraction component and a translation drive component for driving the magnetic attraction component to move linearly. The magnetic attraction component includes a horizontal arm and a first longitudinal arm and a second longitudinal arm located at both ends of the length of the horizontal arm. Magnets are provided on the first longitudinal arm and the second longitudinal arm. The translation drive component includes a lead screw motor, the lead screw of the lead screw motor is threadedly connected to the horizontal arm, and the magnetic attraction component is slidably connected to the device frame via a slide rail assembly.

[0014] In some embodiments, the main frame of the device is provided with a cartridge in / out chamber driving component, which is used to drive the cartridge driving device to switch between the cartridge in / out chamber position and the sequencing library preparation position.

[0015] This invention provides a gene sequencing library preparation device, which includes a pipetting device and a cartridge driving device. The cartridge driving device can drive the target layer of the layered cartridge to rotate, thereby enabling the layered cartridge and the first and second pipetting modules configured on the pipetting device above to perform pipetting operations between the reservoir tubes within the layered cartridge, thus completing the entire process of gene sequencing library preparation. This improves the compactness of the device structure and helps to prevent cross-contamination and other adverse phenomena during operation. Attached Figure Description

[0016] Figure 1This is a three-dimensional structural schematic diagram of the gene sequencing library preparation device in an embodiment of the present invention (the outer shell of the device is omitted in the figure). Figure 2 This is a schematic diagram showing the state of the cartridge driving device in the sequencing library preparation device in the sequencing library preparation position in an embodiment of the present invention; Figure 3 This is a schematic diagram showing the state of the cartridge driving device in the cartridge in / out position in the gene sequencing library preparation equipment according to an embodiment of the present invention. Figure 4 yes Figure 1 A three-dimensional structural diagram of the pipetting device in the image from one perspective; Figure 5 yes Figure 4 A front view of the pipetting apparatus in the image; Figure 6 yes Figure 5 A bottom view of the pipetting apparatus in the image; Figure 7 yes Figure 1 A three-dimensional structural diagram of the pipetting device from another perspective; Figure 8 yes Figure 1 A three-dimensional structural diagram of the card box driving device in the middle; Figure 9 yes Figure 8 Top view of the card box drive unit with the top plate hidden; Figure 10 yes Figure 8 A three-dimensional structural diagram of the magnetic suction module.

[0017] The reference numerals in the attached figures are as follows: 1. Pipetting device; 11. Fixed frame; 111. Vertical sliding assembly; 12. Lifting frame; 121. Slotted photoelectric sensor; 13. Rotating frame; 131. Top turntable; 1311. Driven wheel; 1312. Baffle; 1313. Slip ring; 132. Bottom turntable; 1321. Positioning plug; 1331. First guide connecting post; 1332. Second guide connecting post; 141. First pipetting module; 1411. First pipetting tube; 1412. First lead screw motor; 1413. First lifting block; 142. Second pipetting module; 1421. Second pipetting tube; 1422. Second lead screw motor; 1423. Second lifting block; 143. Pipetting pump; 151. Frame lifting drive assembly; 1511. Third lead screw motor; 152. Rotation drive assembly; 1521. Rotary motor; 1522. Output 1. Wheel; 1523. Synchronous belt; 1524. Tensioner seat; 2. Cartridge drive device; 21. Device frame; 211. Top plate; 22. Cartridge connecting ring; 221. Locking groove; 222. Axial limiting component; 223. Synchronous wheel; 231. Bearing; 232. Rotary drive assembly; 2321. Drive motor assembly; 2322. Drive wheel; 2323. Synchronous belt; 241. Heating plate; 242. Cooling plate; 243. First receiving groove; 245. Cooling fan; 25. Heat conduction plate; 251. Second receiving groove; 261. Screw motor; 2621. Cross arm; 2622. First longitudinal arm; 2623. Second longitudinal arm; 2624. Magnet; 263. Slide rail assembly; 2641. Induction baffle; 2642. Slotted photoelectric sensor; 3. Main frame of equipment; 31. Inlet / outlet screw motor; 100. Layered card box. Detailed Implementation

[0018] See also Figures 1 to 10 As shown, according to an embodiment of the present invention, a gene sequencing library preparation device is provided, including a main frame 3. The main frame 3 is provided with a pipetting device 1 and a cartridge driving device 2. The pipetting device 1 is located in the top region of the cartridge driving device 2. The cartridge driving device 2 is used to carry the layered cartridge 100 and drive the target layer of the layered cartridge 100 to rotate. The pipetting device 1 includes a first pipetting module 141 and a second pipetting module 142 to pipette liquid between the storage tubes in the layered cartridge 100 via the first pipetting module 141 and the second pipetting module 142.

[0019] In this technical solution, the gene sequencing library preparation equipment includes a pipetting device 1 and a cartridge driving device 2. The cartridge driving device 2 can drive the target layer of the layered cartridge 100 to rotate, thereby enabling the layered cartridge 100 to cooperate with the first pipetting module 141 and the second pipetting module 142 configured on the pipetting device 1 above to perform pipetting operations between the liquid storage tubes in the layered cartridge 100, thus completing the entire process of gene sequencing library preparation. This improves the structural compactness of the equipment and also helps to prevent adverse phenomena such as cross-contamination during the operation.

[0020] See also Figures 4 to 7 As shown, the pipetting device 1 includes a fixed frame 11, a lifting frame 12, and a rotating frame 13. The first pipetting module 141 and the second pipetting module 142 are mounted on the rotating frame 13. In one specific embodiment, the first pipetting module 141 is used for pipetting in the library construction process of biological samples, and the second pipetting module 142 is used for pipetting in the nucleic acid extraction process of biological samples. It also includes a frame lifting drive assembly 151 assembled on the fixed frame 11 and a rotating drive assembly 152 assembled on the lifting frame 12. 151 is used to drive the lifting frame 12 to move up and down relative to the fixed frame 11 in the vertical direction. Specifically, the fixed frame 11 and the lifting frame 12 are relatively slidably connected by a vertical sliding component 111. The aforementioned vertical sliding component 111 can be, for example, a commercially available linear guide assembly. The rotating frame 13 is assembled on the lifting frame 12 and can rotate around the first vertical axis (not indicated in the figure) under the drive of the rotating drive component 152 to change the position of the first pipetting module 141 and the second pipetting module 142 in the circumferential direction.

[0021] In this technical solution, on the one hand, at least two pipetting modules, a first pipetting module 141 and a second pipetting module 142, are simultaneously installed on the rotating frame 13 for different operational processes of biological samples. This integrates different pipetting modules on the same frame, allowing the use of a single device to meet the configuration requirements of multiple pipettes for complex biological experimental processes such as gene library construction, without the need for multiple modules to work together. This simplifies the structural design, reduces the size of the equipment, and improves its compactness. On the other hand, the rotating frame 13 is rotatably connected to the lifting frame 12, while the lifting frame 12 is slidably connected to the fixed frame 11. This allows the rotating frame 13 to be rotated while the lifting frame 12 drives the rotating frame 13 to rise and fall as a whole, thereby enabling flexible and efficient adjustment of the height and circumferential position of the first pipetting module 141 and the second pipetting module 142.

[0022] In some embodiments, the first pipetting module 141 includes a first pipette 1411, the aspiration axis of which is located on a first cylindrical surface with the first vertical axis as its central axis. The second pipetting module 142 includes a second pipette 1421, the aspiration axis of which is located on a second cylindrical surface with the first vertical axis as its central axis. The first cylindrical surface is located radially outside the second cylindrical surface. That is, in a specific embodiment, the first pipette 1411 is located radially outside the second pipette 1421. This arrangement ensures that the positions of the first pipette 1411 and the second pipette 1421 correspond to the sample and reagent storage areas of the circular cartridges used with them, achieving a reasonable layout of the pipettes and further improving the compactness of the structural design. It is understandable that the aforementioned first pipette 1411 and second pipette 1421 can insert the tip directly below the lifting frame 12 when the lifting frame 12 descends to the target height, that is, to realize the tip being fitted onto the corresponding pipette, thereby facilitating subsequent pipetting operations on the target position of reagents and / or samples.

[0023] In some embodiments, the rotating frame 13 includes a top turntable 131 and a bottom turntable 132, both of which are rotatably supported on the lifting frame 12. The first pipetting module 141 further includes a first lead screw motor 1412 and a first lifting block 1413 that can be driven to rise and fall by its lead screw. The first pipetting nozzle 1411 is assembled on the first lifting block 1413. The second pipetting module 142 further includes a second lead screw motor 1422 and a second lifting block 1423 that can be driven to rise and fall by its lead screw. The second pipetting nozzle 1421 is assembled on the second lifting block 1423. Specifically, corresponding motor mounting plates (not indicated in the figure) are respectively provided on the bottom surface of the top turntable 131 or the top surface of the bottom turntable 132. The aforementioned first lead screw motor 1412 and second lead screw motor 1422 are assembled on the corresponding motor mounting plates.

[0024] In this technical solution, by setting a first lead screw motor 1412 and a second lead screw motor 1422 to drive the first lifting block 1413 and the second lifting block 1423 respectively, the lifting displacement control is precise and flexible.

[0025] To further ensure the smooth and reliable lifting of the first lifting block 1413 and the second lifting block 1423, in some embodiments, a first guide connecting post 1331 and a second guide connecting post 1332 are provided between the top turntable 131 and the bottom turntable 132. The first lifting block 1413 is slidably connected to the first guide connecting post 1331, and the second lifting block 1423 is slidably connected to the second guide connecting post 1332. In a specific embodiment, two parallel and spaced wire connecting posts are provided for the same lifting block.

[0026] In one specific embodiment, the lifting frame 12 includes a vertical plate (not labeled in the figure), an upper horizontal plate (not labeled in the figure) at the upper end of the vertical plate, and a lower horizontal plate (not labeled in the figure) at the lower end of the vertical plate. The upper and lower horizontal plates each have an upper rotating hole (not labeled in the figure) and a lower rotating hole (not labeled in the figure) that are coaxially aligned. The top turntable 131 is rotatably connected to the upper rotating hole, and the bottom turntable 132 is rotatably connected to the lower rotating hole. It is understood that corresponding bearings should be provided between the top turntable 131 and the bottom turntable 132 and their respective rotating holes to achieve the rotatable connection. The bearings should be selected based on their axial load-bearing capacity to ensure the axial stability and reliability of the rotating frame 13 and its assembled components. In some feasible implementations, corresponding axial limiting structures (e.g., limiting discs) should also be provided for the top turntable 131 and the bottom turntable 132 and their corresponding bearing positions.

[0027] In some embodiments, the bottom surface of the bottom turntable 132 is provided with a plurality of positioning plugs 1321. Each positioning plug 1321 is evenly spaced along the circumferential direction of the first vertical axis, and its distribution circle is the first cylindrical surface. By providing a plurality of positioning plugs 1321, they can be inserted one by one into the positioning holes on the disc chip corresponding to the position below, thereby ensuring the stability of the relative positional relationship between the chip and the rotating frame 13.

[0028] In one specific implementation, a pipetting pump 143 is provided on the top surface of the bottom turntable 132. The pipetting pump 143 is controllably connected to the first pipetting nozzle 1411 to enable the first pipetting nozzle 1411 to perform pipetting using a vacuum suction method. The vacuum suction method ensures a large suction volume of the first pipetting nozzle 1411, thereby improving work efficiency. And / or, the second pipetting nozzle 1421 performs pipetting using a piston suction method, which ensures the accuracy of quantitative pipetting. The second pipetting nozzle 1421 also has a controllable retractable magnetic sleeve. Thus, the second pipetting nozzle 1421 not only performs pipetting but also has the ability to adsorb / desorb magnetic beads in the nucleic acid extraction process, further simplifying the device structure design.

[0029] In some embodiments, a driven wheel 1311 is fixedly connected to the top side of the top turntable 131. The rotary drive assembly 152 includes a rotary motor 1521, which has an output wheel 1522. The rotary drive assembly 152 also includes a synchronous belt 1523 tensioned between the output wheel 1522 and the driven wheel 1311. The aforementioned synchronous belt 1523 is preferably a toothed belt. In this technical solution, the rotary motor 1521 drives the rotation of the rotating frame 13 in sequence via the output wheel 1522, the synchronous belt 1523, the driven wheel 131, and the top turntable 131. The structure is simple and compact.

[0030] In some embodiments, the fixed frame 11 has perforated areas (not shown in the figure) extending through its opposite sides, and the lifting frame 12 has a protrusion extending from the first side of the fixed frame 11 through the perforated areas into the second side of the fixed frame 11. The rotary drive assembly 152 is assembled on the protrusion, and the frame lifting drive assembly 151 is located on the second side. Specifically, the frame lifting drive assembly 151 includes a third lead screw motor 1511, and the output lead screw of the third lead screw motor 1511 is threadedly connected to the protrusion.

[0031] In this technical solution, the frame lifting drive assembly 151 is set in the second side area of ​​the fixed frame 11, making the structural layout of the device more reasonable.

[0032] In some embodiments, the rotary motor 1521 is assembled onto the protrusion via a tensioning seat 1524, on which a displacement adjustment groove (not shown in the figure) is formed. The tensioning seat 1524 is assembled into the displacement adjustment groove, and the distance between the tensioning seat 1524 and the driven wheel 1311 can be adjusted, thereby ensuring the accuracy of power transmission of the synchronous belt 1523.

[0033] In some embodiments, the driven wheel 1311 is provided with a baffle 1312 extending radially outward therefrom, and the lifting frame 12 is provided with a slotted photoelectric sensor 121 at a position that matches the height of the baffle 1312, so that the baffle 1312 and the slotted photoelectric sensor 121 can pass through each other.

[0034] In some embodiments, the driven wheel 1311 is provided with an electric slip ring 1313 in the central region to enable electrical connection between the electrical cables inside the rotating body and the electrical cables on the external fixed structure.

[0035] See also Figures 8 to 9As shown, the card holder driving device 2 includes a device frame 21, which includes a top plate 211. An assembly through hole (not shown in the figure) is formed on the top plate 211. A card holder connecting ring 22 is provided in the assembly through hole. The card holder connecting ring 22 is assembled in the assembly through hole by a bearing 231. A positioning groove 221 is provided on the inner ring wall of the card holder connecting ring 221. The position, size and number of the aforementioned positioning grooves 221 match the corresponding card protrusions on the card holder. In a specific embodiment, there are two positioning grooves 221. The two positioning grooves 221 are symmetrical about the rotation axis of the bearing 231. A rotation drive assembly 232 is also provided in the device frame 21. The rotation drive assembly 232 is used to drive the card holder connecting ring 22 to rotate relative to the top plate 211.

[0036] In this technical solution, the card holder connecting ring 22 is connected to the layered reagent card holder through the card holder groove 221. At the same time, the card holder connecting ring 22 is driven to rotate by a preset angle through the rotation drive component 232, and cooperates with the pipette of the corresponding liquid preparation device. This enables the movement and reaction of liquids (samples or reagents) in the storage tubes at different circumferential positions on the layered card holder, thereby improving the structural compactness of the device.

[0037] In some embodiments, the top surface of the cartridge connecting ring 22 has an axial limiting member 222, which is located on the top side end face of the bearing 231. Specifically, the axial limiting member 222 extends radially outward along the cartridge connecting ring 22 to form an ear structure. The cartridge connecting ring 22 is seated on the top side end face of the inner ring of the bearing 231 by means of the axial limiting member 222. In a specific embodiment, there are two symmetrically arranged axial limiting members 222 to ensure reliable support for the cartridge connecting ring 22. The axial limiting member 222 can be detachably connected to the cartridge connecting ring 22 by screws.

[0038] In some embodiments, the bottom end of the card box connecting ring 22 is provided with a synchronous pulley 223. The rotary drive assembly 232 includes a drive motor assembly 2321, a drive pulley 2322, and a synchronous belt 2323. In a specific embodiment, the synchronous belt 2323 is a belt with teeth on the inner side. The drive pulley 2322 is fitted on the output shaft of the drive motor assembly 2321. The synchronous belt 2323 is tensioned on the drive pulley 2322 and the synchronous pulley 223. The drive motor assembly 2321 may specifically include a rotary motor and a reducer. In this case, the output shaft is also the output shaft of the reducer. In a preferred embodiment, the motor shaft of the rotary motor is orthogonal to the output shaft, that is, perpendicular. This makes the entire drive motor assembly 2321 arranged horizontally (that is, the rotation axis of the motor shaft is parallel to the horizontal plane). The height of the device frame 21 can be designed to be smaller, and the device structure is more compact.

[0039] In this technical solution, the belt drive mechanism is formed by the synchronous belt 2323, the drive wheel 2322 and the synchronous pulley 223, which makes the structural layout of the rotary drive component 232 more flexible.

[0040] In some embodiments, the device frame 21 is further provided with a first temperature control module (not labeled in the figure), the first temperature control module includes a heating unit (not labeled in the figure) and a refrigeration unit (not labeled in the figure), the heating unit and the refrigeration unit are arranged circumferentially around the rotation axis of the card box connecting ring 22.

[0041] In this technical solution, the first temperature control module has both a heating unit and a refrigeration unit. The liquid storage tubes that need to be heated and those that need to be refrigerated on the layered reagent cartridges can be respectively connected to the aforementioned heating unit and refrigeration unit, thereby achieving heating or refrigeration (cooling) of the liquid and meeting the corresponding sample processing requirements.

[0042] In some embodiments, the heating unit includes a heating plate 241 and a Peltier heating element (not shown) located below the heating plate 241, and the refrigeration unit includes a cooling plate 242 and a Peltier cooling element (not shown) located below the cooling plate 242. A plurality of first receiving grooves 243 are formed on the top surfaces of the heating plate 241 and the cooling plate 242, and the corresponding liquid storage tubes are accommodated in the corresponding first receiving grooves 243.

[0043] In this technical solution, Peltier heating elements and Peltier cooling elements are used to adjust the heating and refrigeration temperatures respectively, which has the advantages of high temperature control accuracy and high temperature adjustment response rate. At this time, because the temperature control of the Peltier heating element is more precise, each liquid storage tube in the PCR area on the layered cartridge can be placed in the corresponding first receiving groove 243.

[0044] In some embodiments, the first temperature control module further includes a heat sink (not shown in the figure), wherein the cold end of the Peltier heating element and the hot end of the Peltier cooling element are thermally coupled to the heat sink. That is, the cold end of the Peltier heating element and the hot end of the Peltier cooling element are both connected to the top side surface of the heat sink. This can further simplify the structural design and improve the compactness of the device while meeting the heat dissipation requirements of the Peltier semiconductor element. The aforementioned heat sink is, for example, a finned heat sink.

[0045] In some embodiments, the first temperature control module further includes a cooling fan 245, and the cooling fan 245 and the radiator are both assembled within the device frame 21. The cooling fan 245 is used to drive airflow through the radiator to achieve efficient air cooling of the radiator.

[0046] In some embodiments, a second temperature control module (not indicated in the figure) is also provided within the device frame 21, located in the area between the heating unit and the refrigeration unit. The second temperature control module includes a heat-conducting plate 25, which extends along the diameter direction of the bearing 231. An electric heating film (which can be a conventional resistance wire film) is provided on the bottom surface of the heat-conducting plate 25. A plurality of second receiving grooves 251 are provided on the top surface of the heat-conducting plate 25, and the liquid storage tubes at corresponding positions in the layered card box are accommodated in each of the second receiving grooves 251.

[0047] In this technical solution, a second temperature control module using an electric heating film is installed inside the device frame 21, which can heat and insulate the corresponding liquid storage tube, thereby reducing the device design cost.

[0048] In some embodiments, the device frame 21 is further provided with a magnetic attraction module (not labeled in the figure). The magnetic attraction module includes a magnetic attraction component (not labeled in the figure) and a translation drive component (not labeled in the figure) for driving the magnetic attraction component to move linearly. The aforementioned magnetic attraction component is used to attract magnetic beads in the liquid storage tube that it contacts, so as to facilitate the cleaning, purification and other operations of the corresponding samples.

[0049] In some embodiments, the magnetic suction assembly includes a horizontal arm 2621 and a first vertical arm 2622 and a second vertical arm 2623 located at both ends of the horizontal arm 2621, thus forming an I-shape in appearance. The first vertical arm 2622 and the second vertical arm 2623 are respectively located on opposite sides of the layered card box. Magnets 2624 (permanent magnets) are provided on both the first vertical arm 2622 and the second vertical arm 2623. The translation drive assembly includes a lead screw motor 261, the lead screw of which is threadedly connected to the horizontal arm 2621. The magnetic suction assembly is slidably connected to the device frame 21 via a slide rail assembly 263. This allows the alternating use of two sets of magnets 2624 using the same lead screw motor 261, further simplifying the structural design. In specific applications, the lead screw motor 261 can be controlled to rotate forward to drive the magnetic suction assembly to extend. At this time, the magnets 2624 on the first vertical arm 2622... The magnetic beads inside the storage tube are attracted by the magnetic wall of the layered card box corresponding to its position. At the same time, the magnet 2624 of the second vertical arm 2623 does not contact the outer wall of the storage tube and does not attract the magnetic beads. Conversely, when the lead screw motor 261 reverses and drives the magnetic attraction assembly to retract, the magnet 2624 of the second vertical arm 2623 contacts the outer wall of the storage tube corresponding to the layered card box, attracting the magnetic beads inside the storage tube. Meanwhile, the magnet 2624 of the first vertical arm 2622 does not contact the outer wall of the storage tube and does not attract the magnetic beads. This achieves alternating contact between the magnet 2624 of the first vertical arm 2622 and the outer wall of the storage tube. Of course, according to the actual testing requirements, the magnets 2624 of the first vertical arm 2622 and the second vertical arm 2623 can also be in a completely detached, non-attractive state where they do not contact the outer wall of the storage tube at the same time.

[0050] In one specific embodiment, each of the magnets 2624 has an arc-shaped groove on its side facing the liquid storage tube that matches the outer wall surface of the liquid storage tube, which can ensure close contact between the magnet 2624 and the outer wall of each liquid storage tube and ensure the reliability of magnetic bead adsorption.

[0051] To ensure the positional accuracy of the magnet 2624, a sensing baffle 2641 is provided on the second longitudinal arm 2623, and a slotted photoelectric sensor 2642 is provided on the device frame 21. This allows for real-time detection of the position of the second longitudinal arm 2623. In a specific embodiment, when the aforementioned slotted photoelectric sensor 2642 senses the sensing baffle 2641, it indicates that the magnet 2624 of the first longitudinal arm 2622 is in contact with the outer wall of the corresponding liquid storage tube. This state can be defined as the initial position of the lead screw motor 261.

[0052] It will be readily understood by those skilled in the art that the aforementioned advantageous methods can be freely combined and superimposed without conflict.

[0053] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention. The above are merely preferred embodiments of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications should also be considered within the protection scope of the present invention.

Claims

1. A genetic sequencing library preparation apparatus, comprising: The device body frame (3) is provided with a pipetting device (1) and a cartridge driving device (2), the pipetting device (1) is located at the top area of the cartridge driving device (2), wherein the cartridge driving device (2) is used for carrying a layered cartridge (100) and driving the target layer of the layered cartridge (100) to rotate, and the pipetting device (1) comprises a first pipetting module (141) and a second pipetting module (142) to pipette between the liquid storage tubes in the layered cartridge (100) through the first pipetting module (141) and the second pipetting module (142).

2. The genetic sequencing library preparation device of claim 1, wherein, The pipetting device (1) further comprises a fixed frame (11), a lifting frame (12) and a rotating frame (13), the first pipetting module (141) and the second pipetting module (142) are assembled on the rotating frame (13), the pipetting device (1) further comprises a frame lifting driving assembly (151) assembled on the fixed frame (11) and a rotating driving assembly (152) assembled on the lifting frame (12), the frame lifting driving assembly (151) is used for driving the lifting frame (12) to lift along the vertical direction relative to the fixed frame (11), and the rotating frame (13) is assembled on the lifting frame (12) and can rotate around a first vertical axis under the driving of the rotating driving assembly (152) to change the position of the first pipetting module (141) and the second pipetting module (142) in the circumferential direction.

3. The genetic sequencing library preparation device of claim 2, wherein, The first pipetting module (141) comprises a first pipetting gun (1411), the suction axis of the first pipetting gun (1411) is located on a first cylindrical surface with the first vertical axis as the central axis, the second pipetting module (142) comprises a second pipetting gun (1421), the suction axis of the second pipetting gun (1421) is located on a second cylindrical surface with the first vertical axis as the central axis, and the first cylindrical surface is located radially outside the second cylindrical surface, the rotating frame (13) comprises a top turntable (131) and a bottom turntable (132), both the top turntable (131) and the bottom turntable (132) are rotationally supported on the lifting frame (12), the first pipetting module (141) further comprises a first lead screw motor (1412) and a first lifting block (1413) capable of being driven to lift by the lead screw of the first lead screw motor (1412), and the first pipetting gun (1411) is assembled on the first lifting block (1413), the second pipetting module (142) further comprises a second lead screw motor (1422) and a second lifting block (1423) capable of being driven to lift by the lead screw of the second lead screw motor (1422), and the second pipetting gun (1421) is assembled on the second lifting block (1423).

4. The genetic sequencing library preparation device of claim 3, wherein, The first guide connecting column (1331) and the second guide connecting column (1332) are arranged between the top rotary disc (131) and the bottom rotary disc (132), the first lifting block (1413) is in sliding connection with the first guide connecting column (1331), and the second lifting block (1423) is in sliding connection with the second guide connecting column (1332); and / or the lifting frame (12) comprises a vertical plate, an upper horizontal plate at the upper end of the vertical plate and a lower horizontal plate at the lower end of the vertical plate, the upper horizontal plate and the lower horizontal plate are respectively provided with upper rotary holes and lower rotary holes coaxial with each other, the top rotary disc (131) is in rotary connection with the upper rotary holes, and the bottom rotary disc (132) is in rotary connection with the lower rotary holes; and / or a plurality of positioning plugs (1321) are arranged on the top surface of the bottom rotary disc (132), the positioning plugs (1321) are uniformly and spacedly arranged along the circumferential direction of the first vertical axis, and the distribution circle of the positioning plugs (1321) is the first cylindrical surface.

5. The genetic sequencing library preparation device of claim 3, wherein, The top surface of the bottom rotary disc (132) is provided with a pipette pump (143), the pipette pump (143) is in controllable communication with the first pipette gun (1411) to realize pipetting of the first pipette gun (1411) in a vacuum suction pipetting mode; and / or the second pipette gun (1421) is used for pipetting in a piston suction pipetting mode, and the second pipette gun (1421) further has a magnetic sleeve capable of being controlled to stretch and retract.

6. The genetic sequencing library preparation device of claim 1, wherein, The card box driving device (2) comprises a device frame (21), the device frame (21) comprises a top plate (211), an assembly through hole is formed in the top plate (211), a card box connecting ring (22) is arranged in the assembly through hole, the card box connecting ring (22) is assembled in the assembly through hole through a bearing (231), a clamping groove (221) is arranged on the inner ring wall of the card box connecting ring (22), and a rotary driving assembly (232) is further arranged in the device frame (21), the rotary driving assembly (232) is used for driving the card box connecting ring (22) to rotate relative to the top plate (211).

7. The genetic sequencing library preparation device of claim 6, wherein, A first temperature adjusting module is further arranged in the device frame (21), the first temperature adjusting module comprises a heating unit and a refrigeration unit, and the heating unit and the refrigeration unit are arranged in a circumferential direction along the rotation axis of the card box connecting ring (22).

8. The genetic sequencing library preparation device of claim 7, wherein, A second temperature adjusting module is further arranged in the device frame (21), the second temperature adjusting module is arranged in a region between the heating unit and the refrigeration unit, the second temperature adjusting module comprises a heat conduction plate (25), the heat conduction plate (25) extends along the diameter direction of the bearing (231), an electric heating film is arranged on the bottom surface of the heat conduction plate (25), and a plurality of second containing grooves (251) are arranged in the top surface of the heat conduction plate (25).

9. The genetic sequencing library preparation device of claim 6, wherein, The device frame (21) is also provided with a magnetic attraction module, which comprises a magnetic attraction assembly and a translation driving assembly for driving the linear movement of the magnetic attraction assembly. The magnetic attraction assembly comprises a cross arm (2621) and first and second longitudinal arms (2622 and 2623) at both ends of the cross arm (2621). The first and second longitudinal arms (2622 and 2623) are both provided with magnets (2624). The translation driving assembly comprises a lead screw motor (261). The lead screw of the lead screw motor (261) is threadedly connected with the cross arm (2621). The magnetic attraction assembly and the device frame (21) are slidably connected via a slide rail assembly (263).

10. The genetic sequencing library preparation device of claim 1, wherein, The equipment body frame (3) is provided with a card box in-out bin driving assembly, which is used for driving the card box driving device (2) to switch between the card box in-out bin position and the sequencing library construction position.

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